MS022200 (gene) Bitter gourd (TR) v1

BLAST of MS022200 vs. NCBI nr
Match: XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 698.7 bits (1802), Expect = 2.5e-197
Identity = 344/355 (96.90%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHEHARVQMWNYIFGFTE+AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASNSNNSMAP LLLE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWHNLSA IKAKDGTPFEATHGKDLWSYTAADP+HNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
           EGNMFDCIPKA AAFIMWVLHDWNDEDCIKILKNCRDAIPKK GKVIIVEAVID NEENN
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
           L DVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITP+KAVQSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365

BLAST of MS022200 vs. NCBI nr
Match: XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])

HSP 1 Score: 667.2 bits (1720), Expect = 8.1e-188
Identity = 325/355 (91.55%), Postives = 342/355 (96.34%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHE A VQMWNYIFGFT+MAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASN NNSMAPLLLLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           S+P+MLA WHNLSARIKA DGTPFEATHGKDLWSYTAADP+HN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
           EGNMFDCIPKA AAF+M VLHDWNDEDCIKILKNC+DA+PKKTGKVIIVE VID NEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
           LSDVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITPI+A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355

BLAST of MS022200 vs. NCBI nr
Match: XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 580.5 bits (1495), Expect = 1.0e-161
Identity = 281/355 (79.15%), Postives = 313/355 (88.17%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD A KES  KKEE+ E ARVQ+WNYIFGF E+AI+KCAIELKIADTIE+HGS MTLSQL
Sbjct: 1   MDFAFKESIPKKEEEDEQARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           S ALNCS  LLYRILRFL +RGIFK+E T +NLTSY+QTPLSRLLAS++NNSM PL+LLE
Sbjct: 61  SLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           S+PVMLAPWH LS RIKA +GTPFEA HGKDLWS+ AADP HN I N+AM+C ARV+TVP
Sbjct: 121 SSPVMLAPWHRLSDRIKANEGTPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLTVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILE C EIFEG+GSLVDVGGGNGT LSMIVKACPWI+GIN DLPHV+S +QE  GVQHV
Sbjct: 181 AILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
            G+M D IPKA AAF MWVLHDW+DE CIKILKNCRDAIP+KTGKVIIVEAVI+  EENN
Sbjct: 241 SGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
           LSDVGL+LDMVMMAHTN GKERT++EW YVLH+AGFTRYTITPI+AVQSVIQ F+
Sbjct: 301 LSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQGFL 355

BLAST of MS022200 vs. NCBI nr
Match: XP_022952490.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 577.8 bits (1488), Expect = 6.5e-161
Identity = 281/354 (79.38%), Postives = 315/354 (88.98%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAPLLLLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWH LSARIK  +  PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILE CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ +   +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
            GNMFDCIPKA AAF+MWVLH W+DE CIKILK CR+AIPKKTGKVIIVEAV++  EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           LSDVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022200 vs. NCBI nr
Match: XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.1 bits (1481), Expect = 4.2e-160
Identity = 277/354 (78.25%), Postives = 312/354 (88.14%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SS+LNCS  LL+RI+RFL HRGIFK+E T +NLT Y+ TPLSRLL S S NSMAP +LLE
Sbjct: 61  SSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWH+LSARIK  +  PFEA HGKD+WSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMSVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILE CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHV++     +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
            GNMFDCIPKA AAF+MWVLHDW+DE CIKILKNCRDAIPKKTGKVIIVEAV++  +ENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022200 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 180/367 (49.05%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 5   LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++++  K  ++   A + +W Y+FGF ++A  KCAI+LKI + IE+H S  P+TL++LSS
Sbjct: 15  MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSR--LLASNSNNSMAPLLLL 124
           A++ SP  L RI+RFL H+GIFKE  T   L T Y  TPLSR  ++      S+AP +L 
Sbjct: 75  AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134

Query: 125 ESNPVMLAPWHNL----SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAAR 184
           E+ P MLAPW  L    S+ +      PF+A HGKD+WS+   +P  + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194

Query: 185 VITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECV 244
            + VP +   C  +F+GV ++VDVGGG G ++ M+VK  PWI+G NFDLPHV+ VA+   
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254

Query: 245 GVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDV 304
           GV++VEG+MFD IP   A FI WVLHDW D+DCIKILKNC++A+P   GKV+IVE+VI  
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314

Query: 305 NE--------ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAV 355
           N+        +  L  V L+LDMVMMAHT+ GKERT +EW +VL +AGF RY +  I  V
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374

BLAST of MS022200 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.7e-76
Identity = 154/351 (43.87%), Postives = 213/351 (60.68%), Query Frame = 0

Query: 14  EDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSPP---- 73
           E+    + ++W ++F F +   +KCA+EL I D I SHG P+T+S++  +L  + P    
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 74  ---LLYRILRFLTHRGIFKEETTAQ-NLTSYAQTPLSRLLASNSNNSMAPLLLLESNPVM 133
               L RI+R L H+ +F  E   + N   Y  T  S+ L  +S  +++PL+L E+NP++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 134 LAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEG 193
           L PW  L    + K  +PFE  HG ++W    ADP  N   N AM C+   I    +LE 
Sbjct: 125 LKPWQYLGKCAQEK-SSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184

Query: 194 CGEIFEGV-GSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNM 253
             + F G+ GSLVDVGGG G+ ++ IVKA P IQGINFDLPHVV+ A E  GV+HV G+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 254 FDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAI-PKKTGKVIIVEAVIDVNEENNLSD 313
           F  IP+A A  + W+LHDW+DEDC  ILKNC  AI  KK GKVIIV+ V+  +  +    
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDK 304

Query: 314 VGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           +GL+ D++MMAHT  GKERT  EW  +L+ AGF RY +    A   +I+AF
Sbjct: 305 MGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353

BLAST of MS022200 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 6.2e-74
Identity = 161/365 (44.11%), Postives = 222/365 (60.82%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           M+LA  + T    E        +W  I G  +  ++KCA+EL+I D + SH +P+TL+Q+
Sbjct: 1   MELARNDQT----EAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQI 60

Query: 61  SSALNCSPPL----LYRILRFLTHRGIFKEETTAQ-NLTSYAQTPLSRLLASNS----NN 120
           +S++  SP L    L RI+R L  R IF +  +       Y  T  SRLL S +      
Sbjct: 61  ASSVPDSPSLNLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQV 120

Query: 121 SMAPLLLLESNPVMLAPWHNLSARIKAKDGTPFEATH-GKDLWSYTAADPSHNKIFNDAM 180
           ++AP +   ++P + APW  L AR   + G  FE  H G+ LW  +  +P  NK+FND M
Sbjct: 121 TLAPFVAFMTHPYLSAPWSCL-ARCVKEGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGM 180

Query: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
           A  AR+ T+ AIL    ++F G+ SLVDVGG  G S+S IVK+ P I+GIN+DLPHVV+ 
Sbjct: 181 ASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVAT 240

Query: 241 AQECVG-VQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIV 300
           A    G V HV GNMF+ IP A A F+ W+LHDW DEDC+KILKNCR A+P+K GK+IIV
Sbjct: 241 APTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIV 300

Query: 301 EAVIDVNEENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 355
           + V++        D  ++LD+ +MA T +GKERT +EW  VL + GF RY I  I A+ S
Sbjct: 301 DIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPALTS 358

BLAST of MS022200 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.4e-72
Identity = 142/345 (41.16%), Postives = 219/345 (63.48%), Query Frame = 0

Query: 13  EEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNCSP---P 72
           ++D+  ++ ++WN+I+GF E  ++KCA++L +A+ I + G+ MTLS+LSS L   P    
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63

Query: 73  LLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLESNPVMLAPW 132
            LYR++R+L H  +F + +    L  Y   P ++ L    +  M   +L  ++   +APW
Sbjct: 64  ALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPW 123

Query: 133 HNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEI 192
           H L   +  + GT FE   G ++W Y A  P  N++FN+AMA  +R+I + A+++ CG I
Sbjct: 124 HYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MSALVKECGNI 183

Query: 193 FEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIP 252
           F G+ +LVDVGGG GT++  I  A P I+   +DLPHV++ +     V  V G+MF  IP
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243

Query: 253 KAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLLLD 312
           KA A  +  +LHDW+D++CI+ILK C++A+P K GKVIIV+ V++V  E+  + + L LD
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303

Query: 313 MVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           + MM +T  GKERT EEW  ++H AG+  + IT I AVQSVI+A+
Sbjct: 304 LDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345

BLAST of MS022200 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 131/339 (38.64%), Postives = 215/339 (63.42%), Query Frame = 0

Query: 20  RVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSAL---NCSPPLLYRILR 79
           + Q+WN I+GF +  +++CA+E+ IAD I+++   +TL+QL++ L   N S   LYR++R
Sbjct: 20  QAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYLYRMVR 79

Query: 80  FLTHRGIFKEETTAQNLTS-YAQTPLSRLLASNSNNSMAPLLLLESNPVMLAPWHNLSAR 139
           +L H  I ++ET    +   Y+  P+  LL  ++  SM P++L  +    +  WH +   
Sbjct: 80  YLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWHFMKEG 139

Query: 140 IKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEIFEGVGS 199
           +     T FE   G D+W Y   +P  +++FN+ MA   R++T   ++E C + F+G+ S
Sbjct: 140 LGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT-KTLIEDCRDTFQGLDS 199

Query: 200 LVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKAHAAF 259
           LVD+GGGNGT++  I +A P I+   +DLPHVV+ + +   ++ V G+MF  +P A A  
Sbjct: 200 LVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSAQAIL 259

Query: 260 IMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLLLDMVMMAH 319
           +  +LHDW DE+C+ ILK C++AIPK+TGKVIIV+  ++    + L+   L+LD+ M+ +
Sbjct: 260 LKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDIDMLVN 319

Query: 320 TNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           T  G+ERT+++W  +L +AGF  + I PI+A+QSVI+AF
Sbjct: 320 TG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAF 356

BLAST of MS022200 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.2e-197
Identity = 344/355 (96.90%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHEHARVQMWNYIFGFTE+AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASNSNNSMAP LLLE
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWHNLSA IKAKDGTPFEATHGKDLWSYTAADP+HNKIFNDAMACAARVITVP
Sbjct: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV
Sbjct: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
           EGNMFDCIPKA AAFIMWVLHDWNDEDCIKILKNCRDAIPKK GKVIIVEAVID NEENN
Sbjct: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
           L DVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITP+KAVQSVIQAFI
Sbjct: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365

BLAST of MS022200 vs. ExPASy TrEMBL
Match: A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 325/355 (91.55%), Postives = 342/355 (96.34%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MDLALKESTSKKEEDHE A VQMWNYIFGFT+MAIIKCAIELKIADTIESHGSPMTLSQL
Sbjct: 1   MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLT YAQTPLSRLLASN NNSMAPLLLLE
Sbjct: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           S+P+MLA WHNLSARIKA DGTPFEATHGKDLWSYTAADP+HN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
           EGNMFDCIPKA AAF+M VLHDWNDEDCIKILKNC+DA+PKKTGKVIIVE VID NEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAFI 356
           LSDVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITPI+A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355

BLAST of MS022200 vs. ExPASy TrEMBL
Match: A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.1e-161
Identity = 281/354 (79.38%), Postives = 315/354 (88.98%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAPLLLLE
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWH LSARIK  +  PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILE CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ +   +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
            GNMFDCIPKA AAF+MWVLH W+DE CIKILK CR+AIPKKTGKVIIVEAV++  EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           LSDVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353

BLAST of MS022200 vs. ExPASy TrEMBL
Match: A0A6J1GLX9 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455166 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 274/354 (77.40%), Postives = 308/354 (87.01%), Query Frame = 0

Query: 1   MDLALKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 60
           MD ALK+S SKKEE HEHAR+Q+W YIFGF EMAIIKCAIELKI DTI+SHG  MTL +L
Sbjct: 1   MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60

Query: 61  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 120
           SS+LNCS  LLYRI+RFL HRGIFKEE T      YAQTP+SRLLAS+S +SMAPLLL E
Sbjct: 61  SSSLNCSSSLLYRIMRFLVHRGIFKEEPT----KGYAQTPMSRLLASSSGSSMAPLLLFE 120

Query: 121 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 180
           SNPVMLAPWH LSARIK  +  PFEA HGKD+WSY AA+P+HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMSVP 180

Query: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 240
           AILE CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+ INFDLPHV++     + VQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIDVQHV 240

Query: 241 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 300
            GNMFD IPKA AAF+MWVLHDW+DE CIKILKNCR+AIPKKTGKVIIVEAV++  EENN
Sbjct: 241 GGNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEENN 300

Query: 301 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 349

BLAST of MS022200 vs. ExPASy TrEMBL
Match: A0A6J1GLV5 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455165 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.4e-153
Identity = 270/354 (76.27%), Postives = 307/354 (86.72%), Query Frame = 0

Query: 2   DLALKESTSK-KEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQL 61
           D+  K+S  K +EE++   +VQ+W YIFGF EMA+IKCAIELKI DTIE+HG  MTL+ L
Sbjct: 3   DIGSKDSKLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTDL 62

Query: 62  SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTSYAQTPLSRLLASNSNNSMAPLLLLE 121
           SS+LNCSP LL+RILRFL HRGIFKEE T     SYAQTP+SRLLAS+S +SMAPLLL E
Sbjct: 63  SSSLNCSPLLLHRILRFLVHRGIFKEEPT----KSYAQTPMSRLLASSSGSSMAPLLLFE 122

Query: 122 SNPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVP 181
           SNPVMLAPWH+LSARIK  +  PFEA HGKDLWSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 123 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDLWSYAAADPIHNTMFNDAMACSARVMSVP 182

Query: 182 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 241
            +LE CGEIFEGV  LVDVGGGNGTSLSMIVKACPWI+ INFDLPHVV+ +   +GVQHV
Sbjct: 183 TLLEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVVASSPPPIGVQHV 242

Query: 242 EGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENN 301
            GNMFDCIPKA AAF+MWVLHDW+DE CIKILKNCRDAIPKKTGKVIIVEAV++  +ENN
Sbjct: 243 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 302

Query: 302 LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           LSDVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITPI+AVQSVIQAF
Sbjct: 303 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRAVQSVIQAF 352

BLAST of MS022200 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 351.3 bits (900), Expect = 9.2e-97
Identity = 180/367 (49.05%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 5   LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++++  K  ++   A + +W Y+FGF ++A  KCAI+LKI + IE+H S  P+TL++LSS
Sbjct: 15  MEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSS 74

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSR--LLASNSNNSMAPLLLL 124
           A++ SP  L RI+RFL H+GIFKE  T   L T Y  TPLSR  ++      S+AP +L 
Sbjct: 75  AVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLF 134

Query: 125 ESNPVMLAPWHNL----SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAAR 184
           E+ P MLAPW  L    S+ +      PF+A HGKD+WS+   +P  + + N+AMAC AR
Sbjct: 135 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 194

Query: 185 VITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECV 244
            + VP +   C  +F+GV ++VDVGGG G ++ M+VK  PWI+G NFDLPHV+ VA+   
Sbjct: 195 RV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLD 254

Query: 245 GVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDV 304
           GV++VEG+MFD IP   A FI WVLHDW D+DCIKILKNC++A+P   GKV+IVE+VI  
Sbjct: 255 GVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGE 314

Query: 305 NE--------ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAV 355
           N+        +  L  V L+LDMVMMAHT+ GKERT +EW +VL +AGF RY +  I  V
Sbjct: 315 NKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDV 374

BLAST of MS022200 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 299.3 bits (765), Expect = 4.1e-81
Identity = 160/361 (44.32%), Postives = 218/361 (60.39%), Query Frame = 0

Query: 5   LKESTSKKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGS--PMTLSQLSS 64
           ++ES     ++   A + +W Y+FGF ++A  KCAI+LKI + IE+H S  P+TLS+LSS
Sbjct: 1   MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60

Query: 65  ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TSYAQTPLSRLLASNSNNSMAPLLLLES 124
           A++ SP  L RI+RFL H+G+FKE  T   L T Y  TPLSR +                
Sbjct: 61  AVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM--------------- 120

Query: 125 NPVMLAPWHNLSARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMACAARVITVPA 184
                                     HGKDLW++   +  H+++ N+AMAC AR + VP 
Sbjct: 121 ----------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPR 180

Query: 185 ILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVE 244
           +   C  +F+GV ++VDVGGG G ++ ++VK  PWI+G NFDLPHV+ VAQ   GV++VE
Sbjct: 181 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 240

Query: 245 GNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEAVIDVNE---- 304
           G+MFD IP + A  I WVLHDW D+DCIKILKNC++A+    GKV+IVE VI   +    
Sbjct: 241 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMI 300

Query: 305 ----ENNLSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQSVIQA 355
               ++ L  V L LDMVMM HT+ GKERT +EW +VL +AGF RY +     VQS+I A
Sbjct: 301 AEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIA 323

BLAST of MS022200 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 199.9 bits (507), Expect = 3.4e-51
Identity = 120/329 (36.47%), Postives = 189/329 (57.45%), Query Frame = 0

Query: 36  IKCAIELKIADTIESHGSPMTLSQLSSALNC----SPPLLYRILRFLTHRGIF---KEET 95
           +K A+EL + + +  +GSPM+ ++++S L      +P +L RILR LT   +      + 
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 96  TAQNLTS-YAQTPLSRLLASNSNN-SMAPLLLLESNPVMLAPWHNLSARIKAKDGTPFEA 155
           +   +   Y   P+ + L  N +  S+A L L+  + V++  W++L   I    G PF  
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPFNK 155

Query: 156 THGKDLWSYTAADPSHNKIFNDAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTS 215
            +G   + Y   DP  NK+FN+ M+  +  IT+  ILE   + FEG+ SLVDVGGG G +
Sbjct: 156 AYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKILE-TYKGFEGLTSLVDVGGGIGAT 215

Query: 216 LSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDE 275
           L MIV   P ++GINFDLPHV+  A    G++HV G+MF  +PK  A F+ W+ HDW+DE
Sbjct: 216 LKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDE 275

Query: 276 DCIKILKNCRDAIPKKTGKVIIVEAVIDVNEENNLSDVGLL-LDMVMMAHTNKGKERTSE 335
            C+K LKNC +++P + GKVI+ E ++    +++LS   ++ +D +M+AH   GKERT +
Sbjct: 276 HCVKFLKNCYESLP-EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEK 335

Query: 336 EWAYVLHKAGFTRYTITPIKAVQSVIQAF 355
           E+  +   +GF        K ++ V  AF
Sbjct: 336 EFEALAKASGF--------KGIKVVCDAF 352

BLAST of MS022200 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.8e-45
Identity = 125/363 (34.44%), Postives = 199/363 (54.82%), Query Frame = 0

Query: 11  KKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTI--ESHGSPMTLSQLSSALNC-- 70
           ++E D +   +Q  + +       ++K A+EL + DTI   S+G+ ++ S+++ +L    
Sbjct: 21  EQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKP 80

Query: 71  ----SPPLLYRILRFLTHRGIFK--------EETTAQNLTSYAQTPLSR--LLASNSNNS 130
               +P LL R+LR L    I K           T +    YA  P+ +  L  S+ + S
Sbjct: 81  TNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGS 140

Query: 131 MAPLLLLESNPVMLAPWHNL-SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMA 190
           ++ LLLL  + V+L  W NL    ++ KD   F + H   L+ Y ++D   +K+F+ AM+
Sbjct: 141 LSSLLLLLHSQVILKTWTNLKDVILEGKDA--FSSAHDMRLFEYISSDDQFSKLFHRAMS 200

Query: 191 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 250
            ++ ++    + E  G  FE V +LVDVGGG GT L +I    P I+G+NFDL  V++ A
Sbjct: 201 ESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQA 260

Query: 251 QECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEA 310
               GV+HV G+MF  +PK  A F+ W+LHDW DEDCIKILKNC  ++P+K GKVIIVE 
Sbjct: 261 PFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEM 320

Query: 311 VIDVNEENN--LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 353
           +  +  + N    +  L +D++M+   + GKER+  ++  +   +GF    I  +    S
Sbjct: 321 ITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYS 378

BLAST of MS022200 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 176.8 bits (447), Expect = 3.1e-44
Identity = 123/363 (33.88%), Postives = 185/363 (50.96%), Query Frame = 0

Query: 11  KKEEDHEHARVQMWNYIFGFTEMAIIKCAIELKIADTIESHGSPMTLSQLSSALNC---- 70
           ++E D +   +Q  + +       ++K A EL + DTI + G+   LS    A +     
Sbjct: 21  EQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKP 80

Query: 71  ----SPPLLYRILRFLTHRGIFK---EETTAQNLTS-----YAQTPLSR--LLASNSNNS 130
               +P LL R+L  L    I K    ET     T      YA  P+ +  L  S+   S
Sbjct: 81  TNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGS 140

Query: 131 MAPLLLLESNPVMLAPWHNL-SARIKAKDGTPFEATHGKDLWSYTAADPSHNKIFNDAMA 190
           + PL +L    V    W NL    ++ +D   F + HG  ++ Y  +D    ++FN AM+
Sbjct: 141 LVPLFMLLHTQVFFKTWTNLKDVILEGRDA--FNSAHGMKIFEYINSDQPFAELFNRAMS 200

Query: 191 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 250
             + +I    +    G  FE V +LVDVGGGNGT L ++    P I+G+NFDL  V++ A
Sbjct: 201 EPSTMIMKKVLDVYRG--FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQA 260

Query: 251 QECVGVQHVEGNMFDCIPKAHAAFIMWVLHDWNDEDCIKILKNCRDAIPKKTGKVIIVEA 310
               GV+HV G+MF  +PK  A F+ W+LHDW DEDCIKILKNC  ++P+K GK+IIVE 
Sbjct: 261 PFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEF 320

Query: 311 VIDVNEENN--LSDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPIKAVQS 353
           V     +     S+    +D++M+   + GKER+  ++  +   +GF R  I  +    S
Sbjct: 321 VTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYS 378