MS022174 (gene) Bitter gourd (TR) v1

Overview


Name	MS022174
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	FAD_binding_3 domain-containing protein
Location	scaffold47: 1417032 .. 1417217 (-)
RNA-Seq Expression	MS022174
Synteny	MS022174

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGATATAGTGATCGTCGGAGCGGGAATCGCCGGCCTCACCACTTCCTTGGCGCTTCACAGATTAGGTTTCAAAAGCTTAGTCTTAGAAACTTCCGATTCTCTGAGGGCCGCCGGATATGCCTTAACGACGTGGAACAACACTTGGAAGGCTTTGGACGCCTTGGGAATCGCCGATTCCCTCCGC

mRNA sequence

GAAGATATAGTGATCGTCGGAGCGGGAATCGCCGGCCTCACCACTTCCTTGGCGCTTCACAGATTAGGTTTCAAAAGCTTAGTCTTAGAAACTTCCGATTCTCTGAGGGCCGCCGGATATGCCTTAACGACGTGGAACAACACTTGGAAGGCTTTGGACGCCTTGGGAATCGCCGATTCCCTCCGC

Coding sequence (CDS)

GAAGATATAGTGATCGTCGGAGCGGGAATCGCCGGCCTCACCACTTCCTTGGCGCTTCACAGATTAGGTTTCAAAAGCTTAGTCTTAGAAACTTCCGATTCTCTGAGGGCCGCCGGATATGCCTTAACGACGTGGAACAACACTTGGAAGGCTTTGGACGCCTTGGGAATCGCCGATTCCCTCCGC

Protein sequence

EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSLR

Homology

[Close]

BLAST of MS022174 vs. NCBI nr
Match: XP_038887729.1 (monooxygenase 2-like [Benincasa hispida])

HSP 1 Score: 112.8 bits (281), Expect = 1.0e-21
Identity = 56/62 (90.32%), Postives = 59/62 (95.16%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGIAGL+T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALGIADS
Sbjct: 8  EDIVIVGAGIAGLSTALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGIADS 67

Query: 61 LR 63
          LR
Sbjct: 68 LR 69

[Close]

BLAST of MS022174 vs. NCBI nr
Match: XP_023554695.1 (monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGIAGL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 7  EDIVIVGAGIAGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 66

Query: 61 LR 63
          LR
Sbjct: 67 LR 68

[Close]

BLAST of MS022174 vs. NCBI nr
Match: XP_022953131.1 (monooxygenase 2-like isoform X1 [Cucurbita moschata] >XP_022953132.1 monooxygenase 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGIAGL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 7  EDIVIVGAGIAGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 66

Query: 61 LR 63
          LR
Sbjct: 67 LR 68

[Close]

BLAST of MS022174 vs. NCBI nr
Match: KAG6572121.1 (Monooxygenase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGIAGL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 7  EDIVIVGAGIAGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 66

Query: 61 LR 63
          LR
Sbjct: 67 LR 68

[Close]

BLAST of MS022174 vs. NCBI nr
Match: KAG7011774.1 (hpxO, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1   EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
           EDIVIVGAGIAGL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 401 EDIVIVGAGIAGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 460

Query: 61  LR 63
           LR
Sbjct: 461 LR 462

[Close]

BLAST of MS022174 vs. ExPASy Swiss-Prot
Match: O81816 (Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.8e-16
Identity = 37/61 (60.66%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 2  DIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSL 61
          DI+IVGAGI+GL+T++ LHRLG +S+VLE+S++LRA G+A TTW N WKA++ALG++  +
Sbjct: 8  DIIIVGAGISGLSTAVGLHRLGIRSMVLESSETLRATGFAFTTWFNAWKAMEALGVSQHI 67

Query: 62 R 63
          R
Sbjct: 68 R 68

[Close]

BLAST of MS022174 vs. ExPASy Swiss-Prot
Match: Q9FLC2 (Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-15
Identity = 37/62 (59.68%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          +DI+IVGAGI+GL T+L LHRLG +S+VLE+S+ LRA G+AL+ + N WKA++ALGI+  
Sbjct: 7  QDIIIVGAGISGLATALGLHRLGIRSIVLESSEQLRATGFALSLYFNAWKAMEALGISQH 66

Query: 61 LR 63
          +R
Sbjct: 67 IR 68

[Close]

BLAST of MS022174 vs. ExPASy Swiss-Prot
Match: O81815 (Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-12
Identity = 35/60 (58.33%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 3  IVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSLR 62
          IVIVG GIAGL TS+ALHR G KS+VLE ++ +R+ G  + T +N W+ALD LG+ D LR
Sbjct: 6  IVIVGGGIAGLATSIALHRKGIKSVVLERAEKVRSEGAGIGTLSNGWRALDQLGVGDRLR 65

[Close]

BLAST of MS022174 vs. ExPASy Swiss-Prot
Match: Q6F6Y2 (FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=hpxO PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-05
Identity = 21/60 (35.00%), Postives = 39/60 (65.00%), Query Frame = 0


Query: 2  DIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSL 61
          ++VI+GAG+ GLTT +AL + G +  + E ++ +   G A++ W+N  K L+ LG+ + +
Sbjct: 2  NVVIIGAGMGGLTTGIALKKFGHQVTIFEQAEQILPVGAAISLWSNGVKCLNYLGLNEQI 61

[Close]

BLAST of MS022174 vs. ExPASy Swiss-Prot
Match: Q9F131 (3-hydroxybenzoate 6-hydroxylase 1 OS=Pseudomonas alcaligenes OX=43263 GN=xlnD PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.8e-05
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 2  DIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSL 61
          +I+I GAGI GL+ +L L R G +S+VLE +  L   G  +    N + ALDALGI +  
Sbjct: 4  NILIAGAGIGGLSAALGLARKGMRSIVLEKAPELGEIGAGIQLAPNAYHALDALGIGEVA 63

Query: 62 R 63
          R
Sbjct: 64 R 64

[Close]

BLAST of MS022174 vs. ExPASy TrEMBL
Match: A0A6J1GMD2 (monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455625 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 8.5e-22
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGIAGL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 7  EDIVIVGAGIAGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 66

Query: 61 LR 63
          LR
Sbjct: 67 LR 68

[Close]

BLAST of MS022174 vs. ExPASy TrEMBL
Match: A0A0A0K2K7 (FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G014500 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.9e-21
Identity = 54/62 (87.10%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          EDIVIVGAGI+GL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 9  EDIVIVGAGISGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 68

Query: 61 LR 63
          LR
Sbjct: 69 LR 70

[Close]

BLAST of MS022174 vs. ExPASy TrEMBL
Match: A0A5A7U5X4 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G001200 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.5e-21
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          E+IVIVGAGI+GL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 8  EEIVIVGAGISGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 67

Query: 61 LR 63
          LR
Sbjct: 68 LR 69

[Close]

BLAST of MS022174 vs. ExPASy TrEMBL
Match: A0A5D3CL17 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00440 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.5e-21
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          E+IVIVGAGI+GL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 8  EEIVIVGAGISGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 67

Query: 61 LR 63
          LR
Sbjct: 68 LR 69

[Close]

BLAST of MS022174 vs. ExPASy TrEMBL
Match: A0A1S3BIK9 (FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.5e-21
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          E+IVIVGAGI+GL T+L LHRLG +SLVLETSDSLRAAGYALTTWNN WKALDALG+ADS
Sbjct: 8  EEIVIVGAGISGLATALGLHRLGIRSLVLETSDSLRAAGYALTTWNNAWKALDALGVADS 67

Query: 61 LR 63
          LR
Sbjct: 68 LR 69

[Close]

BLAST of MS022174 vs. TAIR 10
Match: AT4G38540.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 85.9 bits (211), Expect = 1.3e-17
Identity = 37/61 (60.66%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 2  DIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSL 61
          DI+IVGAGI+GL+T++ LHRLG +S+VLE+S++LRA G+A TTW N WKA++ALG++  +
Sbjct: 8  DIIIVGAGISGLSTAVGLHRLGIRSMVLESSETLRATGFAFTTWFNAWKAMEALGVSQHI 67

Query: 62 R 63
          R
Sbjct: 68 R 68

[Close]

BLAST of MS022174 vs. TAIR 10
Match: AT5G05320.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-16
Identity = 37/62 (59.68%), Postives = 52/62 (83.87%), Query Frame = 0


Query: 1  EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
          +DI+IVGAGI+GL T+L LHRLG +S+VLE+S+ LRA G+AL+ + N WKA++ALGI+  
Sbjct: 7  QDIIIVGAGISGLATALGLHRLGIRSIVLESSEQLRATGFALSLYFNAWKAMEALGISQH 66

Query: 61 LR 63
          +R
Sbjct: 67 IR 68

[Close]

BLAST of MS022174 vs. TAIR 10
Match: AT2G35660.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.9e-13
Identity = 33/62 (53.23%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 1   EDIVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADS 60
           E +VIVGAGI GL T+++LHRLG +S+VLE ++SLR  G +LT + N W+ LDA+ +   
Sbjct: 45  EKVVIVGAGIGGLATAVSLHRLGIRSVVLEQAESLRTGGTSLTLFKNGWRVLDAISVGPQ 104

Query: 61  LR 63
           LR
Sbjct: 105 LR 106

[Close]

BLAST of MS022174 vs. TAIR 10
Match: AT4G15760.1 (monooxygenase 1 )

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-13
Identity = 35/60 (58.33%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 3  IVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSLR 62
          IVIVG GIAGL TS+ALHR G KS+VLE ++ +R+ G  + T +N W+ALD LG+ D LR
Sbjct: 31 IVIVGGGIAGLATSIALHRKGIKSVVLERAEKVRSEGAGIGTLSNGWRALDQLGVGDRLR 90

[Close]

BLAST of MS022174 vs. TAIR 10
Match: AT4G15760.2 (monooxygenase 1 )

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-13
Identity = 35/60 (58.33%), Postives = 45/60 (75.00%), Query Frame = 0


Query: 3  IVIVGAGIAGLTTSLALHRLGFKSLVLETSDSLRAAGYALTTWNNTWKALDALGIADSLR 62
          IVIVG GIAGL TS+ALHR G KS+VLE ++ +R+ G  + T +N W+ALD LG+ D LR
Sbjct: 6  IVIVGGGIAGLATSIALHRKGIKSVVLERAEKVRSEGAGIGTLSNGWRALDQLGVGDRLR 65

The following BLAST results are available for this feature:

BLAST of MS022174 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887729.1	1.0e-21	90.32	monooxygenase 2-like [Benincasa hispida]	[more]
XP_023554695.1	1.8e-21	88.71	monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022953131.1	1.8e-21	88.71	monooxygenase 2-like isoform X1 [Cucurbita moschata] >XP_022953132.1 monooxygena...	[more]
KAG6572121.1	1.8e-21	88.71	Monooxygenase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7011774.1	1.8e-21	88.71	hpxO, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS022174 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81816	1.8e-16	60.66	Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1	[more]
Q9FLC2	2.6e-15	59.68	Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1	[more]
O81815	3.5e-12	58.33	Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1	[more]
Q6F6Y2	1.4e-05	35.00	FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD4...	[more]
Q9F131	1.8e-05	44.26	3-hydroxybenzoate 6-hydroxylase 1 OS=Pseudomonas alcaligenes OX=43263 GN=xlnD PE...	[more]

BLAST of MS022174 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMD2	8.5e-22	88.71	monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455625 PE...	[more]
A0A0A0K2K7	1.9e-21	87.10	FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G0145...	[more]
A0A5A7U5X4	5.5e-21	85.48	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5D3CL17	5.5e-21	85.48	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BIK9	5.5e-21	85.48	FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=...	[more]

BLAST of MS022174 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38540.1	1.3e-17	60.66	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G05320.1	1.8e-16	59.68	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G35660.1	1.9e-13	53.23	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT4G15760.1	2.5e-13	58.33	monooxygenase 1	[more]
AT4G15760.2	2.5e-13	58.33	monooxygenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 2..62 e-value: 8.1E-17 score: 63.4
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 2..61
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 2..61 e-value: 3.9E-8 score: 32.9
IPR044560	Monooxygenase/2-heptyl-3-hydroxy-4(1H)-quinolone synthase	PANTHER	PTHR45934	FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN	coord: 1..62
None	No IPR available	PANTHER	PTHR45934:SF20	MONOOXYGENASE 2-RELATED	coord: 1..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS022174.1	MS022174.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding