MS021818 (gene) Bitter gourd (TR) v1

BLAST of MS021818 vs. NCBI nr
Match: XP_038899089.1 (F-box protein SKIP23-like [Benincasa hispida])

HSP 1 Score: 510.0 bits (1312), Expect = 1.9e-140
Identity = 263/407 (64.62%), Postives = 310/407 (76.17%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L  DLLQ IS ++TVY DY+RFR VC +WRS VP+ P  LPPQLPCL+IP YH 
Sbjct: 1   MADWTKLPSDLLQYISDHLTVYSDYLRFRVVCRSWRSSVPKIPHRLPPQLPCLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF F+DN+IHFLHLPEAS  KRRCGSSHGWLAILDETPTILLLNP+T+A LRLPPL
Sbjct: 61  SQCGLFNFTDNKIHFLHLPEASLRKRRCGSSHGWLAILDETPTILLLNPLTRAKLRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+P NQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYTRNLRQMRDSFVKKIVLSSSPWNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSG  GSW+FVDD PS CED ++S  DGVFYAVD YGVVSVI+LR SR +
Sbjct: 181 LNHSGDLAFCRSG--GSWTFVDDAPSDCEDIVFS--DGVFYAVDKYGVVSVINLRGSRSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMR--SRTLRFEV 300
           VS +AT  R +    +Y+V+ G ELLLV RYLD     ++N+G++   +    RT+RFEV
Sbjct: 241 VSLVAT-ERQLAGDIQYLVKLGQELLLVSRYLD-----IVNDGMDDDLVSVIYRTVRFEV 300

Query: 301 FRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQ 360
           FRMEWEGP WEK+E+LN MALF+G NSS+AFSAADF  IS NCIYYTDDY    Y G+ +
Sbjct: 301 FRMEWEGPRWEKVENLNEMALFIGGNSSMAFSAADFAGISGNCIYYTDDYSDNDYQGQGE 360

Query: 361 RLDVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
             D+GIFRL DE+FEP+      + S SS  R  L LPPIW++PNPC
Sbjct: 361 EPDMGIFRLCDESFEPL-----PYYSGSSHSRRRL-LPPIWVTPNPC 391

BLAST of MS021818 vs. NCBI nr
Match: XP_008453081.1 (PREDICTED: F-box protein SKIP23-like [Cucumis melo] >ADN34130.1 ubiquitin-protein ligase [Cucumis melo subsp. melo] >KAA0064619.1 F-box protein SKIP23-like [Cucumis melo var. makuwa] >TYK19973.1 F-box protein SKIP23-like [Cucumis melo var. makuwa])

HSP 1 Score: 508.8 bits (1309), Expect = 4.2e-140
Identity = 263/407 (64.62%), Postives = 307/407 (75.43%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWTEL PDLLQ IS  +TVY DY+RFR VC +WRS VP+ P  LPPQLPCL+IP YH 
Sbjct: 1   MADWTELPPDLLQQISDYLTVYSDYLRFRVVCQSWRSSVPKIPHRLPPQLPCLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
             CGLF FSDN+IHFL+LPEAS  KR CGSSHGWL ILDETP+ILLLNP+T+A  RLPPL
Sbjct: 61  CRCGLFNFSDNKIHFLYLPEASLRKRPCGSSHGWLTILDETPSILLLNPLTRAKFRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYTRNLRQMRDSFVKKIVLSSSPSNQNDFLAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG GSW+FVDD PS CED IY+  DGVFYAVD YGVVS++DLR SR +
Sbjct: 181 LNHSGDLAFCRSGG-GSWTFVDDAPSDCEDVIYT--DGVFYAVDKYGVVSLMDLRGSRSQ 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPF--MRSRTLRFEV 300
           VS +AT  R +    +Y+VR G ELLLV RYLD     ++N+G++     +  RT+RFEV
Sbjct: 241 VSLVAT-ERQLAGDIQYLVRLGQELLLVSRYLD-----IVNDGMDDELISVMYRTVRFEV 300

Query: 301 FRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQ 360
           FRMEWEGP WEK+E+LN MALF+G NSS+AFSAADF EIS NCIYYTDDY    Y G+ +
Sbjct: 301 FRMEWEGPRWEKVENLNEMALFVGGNSSMAFSAADFGEISGNCIYYTDDYSDSDYQGQGE 360

Query: 361 RLDVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
             D+GIFRL DE+FEP+      + S  S  R  L LPPIW++PNPC
Sbjct: 361 EPDMGIFRLCDESFEPL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. NCBI nr
Match: XP_023536028.1 (F-box protein SKIP23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 502.7 bits (1293), Expect = 3.0e-138
Identity = 257/405 (63.46%), Postives = 310/405 (76.54%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L PDLLQ+I+ N+TVY DY+RFR VC +WRS VP+ P  LPPQLP L+IP YH 
Sbjct: 1   MADWTQLPPDLLQVIADNLTVYSDYLRFRVVCQSWRSSVPKIPRRLPPQLPWLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF FSDN+IHFLHLPEAS GKRRCGSSHGWLAILDETPTILLLNP+T+A LRLPPL
Sbjct: 61  SRCGLFNFSDNKIHFLHLPEASLGKRRCGSSHGWLAILDETPTILLLNPLTRAKLRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYSRNLRQMRDSFVKKIVLSSSPSNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG+ SW+FVDD PS CED +YS  DG+FYAVD +GV+SV+DLR SR +
Sbjct: 181 LNHSGDLAFCRSGGE-SWTFVDDAPSDCEDIVYS--DGLFYAVDKHGVISVMDLRGSRSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFR 300
           VS + TG +      +Y+V+ G ELLLV RYLD +   +++E ++  +   RT+RFEVFR
Sbjct: 241 VSLVTTGSQ-SAGDIQYLVKLGRELLLVSRYLDIINDAMVDELISVIY---RTVRFEVFR 300

Query: 301 MEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQRL 360
           MEWEGP WEK+E+L+ MALF+G NSS+AFSA+DF  IS NCIYYTDDY    Y G+ +  
Sbjct: 301 MEWEGPRWEKVENLDEMALFVGGNSSMAFSASDFGGISGNCIYYTDDYSDTDYQGQGEEP 360

Query: 361 DVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
           D+GIFRL D +FE +      + S  S  R  L LPPIW++PNPC
Sbjct: 361 DMGIFRLCDGSFEQL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. NCBI nr
Match: XP_022975457.1 (F-box protein SKIP23-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 9.7e-137
Identity = 254/405 (62.72%), Postives = 307/405 (75.80%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L PDLLQ+IS N+ VY DY+RFR VC +WRS VP+ P  LPPQLP L+IP YH 
Sbjct: 1   MADWTQLPPDLLQVISDNLNVYSDYLRFRVVCQSWRSSVPKIPRRLPPQLPWLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF FSDN+IHFLHLPEAS G+RRCGSSHGWL ILDETPTILLLNP+T+A  RLPPL
Sbjct: 61  SRCGLFNFSDNKIHFLHLPEASLGRRRCGSSHGWLVILDETPTILLLNPLTRAKCRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRIGREYLIRTPTGHIYSRNLRQMRDTFVKKIVLSSSPSNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG+ SW+FVDD PS CED +YS  DG+FYAVD +GV+SV+DLR SR +
Sbjct: 181 LNHSGDLAFCRSGGE-SWTFVDDAPSDCEDIVYS--DGLFYAVDKHGVISVMDLRGSRSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFR 300
           VS + TG +      +Y+V+ G ELLLV RYLD +   +++E ++  +   RT+RFEVFR
Sbjct: 241 VSLVTTGSQ-SAGDIQYLVKLGRELLLVSRYLDIINDAMVDELISVIY---RTVRFEVFR 300

Query: 301 MEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQRL 360
           MEWEGP WEK+E+L+ MALF+G NSS+AFSA+DF  IS NCIYYTDDY    Y G+ +  
Sbjct: 301 MEWEGPRWEKVENLDEMALFVGGNSSMAFSASDFGGISGNCIYYTDDYSDNDYQGQGEEP 360

Query: 361 DVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
           D+GIFRL D +FE +      + S  S  R  L LPPIW++PNPC
Sbjct: 361 DMGIFRLCDGSFEQL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. NCBI nr
Match: XP_022936291.1 (F-box protein SKIP23-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 495.0 bits (1273), Expect = 6.3e-136
Identity = 252/405 (62.22%), Postives = 308/405 (76.05%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L PDLLQ+I+ N+TVY DY+RFR VC +WRS VP+ P  LPPQLP L+IP YH 
Sbjct: 1   MADWTQLPPDLLQVIADNLTVYSDYLRFRVVCQSWRSSVPKIPRRLPPQLPWLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF FSDN+IHFLHLPEAS GKRRCGSSHGWLAI+DE PTILLLNP+T+A LRLPPL
Sbjct: 61  SRCGLFNFSDNKIHFLHLPEASLGKRRCGSSHGWLAIVDENPTILLLNPLTRAKLRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD F+ KIVLSS+PSNQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYSRNLRQMRDSFLKKIVLSSSPSNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG+ SW+FV+D PS CED +YS  DG+FYAVD +GV+SV+DLR S  +
Sbjct: 181 LNHSGDLAFCRSGGE-SWTFVNDAPSDCEDIVYS--DGLFYAVDKHGVISVMDLRGSHSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFR 300
           VS + TG +      +Y+V+ G ELLLV RYLD +   +++E ++  +   RT+RFEVFR
Sbjct: 241 VSLVTTGSQ-SAGDIQYLVKLGRELLLVSRYLDIINDAMVDELISVIY---RTVRFEVFR 300

Query: 301 MEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQRL 360
           MEWEGP WEK+E+L+ MALF+G NSS+AFSA+DF  IS NCIYYTDDY    Y G+ +  
Sbjct: 301 MEWEGPRWEKVENLDEMALFVGGNSSMAFSASDFGGISGNCIYYTDDYSDNDYQGQGEEP 360

Query: 361 DVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
           D+GIFRL D +FE +      + S  S  R  L LPPIW++PNPC
Sbjct: 361 DMGIFRLCDGSFEQL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. ExPASy Swiss-Prot
Match: Q3EBZ2 (F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.3e-24
Identity = 110/400 (27.50%), Postives = 179/400 (44.75%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRS---PVPENPSHLPPQLPCLLIPR 60
           M DW+ L  DLL LIS+++    D I+FR+VC +WRS   P    P+H  P LP      
Sbjct: 1   MVDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILP------ 60

Query: 61  YHCSSCGLFIFSDNRIHFLHLPEAS------HGKRRCGSSHGWLAILDE----TPTILLL 120
                 G  +F D+ + F  L + S      H       S GWL  ++E       + LL
Sbjct: 61  ----DNGGSLFPDSAVGF-RLSQRSILLIKPHEPCIESDSFGWLIKVEEDLNVPRKVTLL 120

Query: 121 NPMTKATLRLPPLSTFPNVV---SFDSSRSGREYEVREF--AGQIRRYSLRKMRDYFVNK 180
           +P+      +P    FP V+    F     GRE+++  F   G I       +   ++ K
Sbjct: 121 DPLCDTRNSIP--ENFPRVLDMSKFKVRELGREFKLHYFNTVGDI-------VESLYLEK 180

Query: 181 IVLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYA 240
            V+     + +    +  +   G LA +RS  D +W+ ++D PS  +D +    DG F+A
Sbjct: 181 AVVKYLDCDGDYKFVLLTIHVSGKLAVFRS-WDRAWTVINDMPSPYDDVML--FDGRFFA 240

Query: 241 VDCYGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVRSGDELLLVIRYLD----DMFSR 300
           VD  G   V+D   S  K++ +A+        K++++ S  E+LLV  YL     +    
Sbjct: 241 VDNNGRTVVVDY--SSLKLTLVAS--PVFGGDKKFLIESCGEMLLVDMYLSLEAVEGDPG 300

Query: 301 VMNEGLNCP--FMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAAD-FP 360
            + E    P  +M  RT++F+V+R       W  +  L    LFLG +S+ + SA+D  P
Sbjct: 301 FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILP 360

Query: 361 EISENCIYYTDDYCSETYIGECQRLDVGIFRLSDENFEPV 376
               + +++  +  +   +G  Q  D+G+F       E V
Sbjct: 361 LCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELV 373

BLAST of MS021818 vs. ExPASy Swiss-Prot
Match: Q9SIX0 (Putative F-box protein At2g16290 OS=Arabidopsis thaliana OX=3702 GN=At2g16290 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.0e-13
Identity = 106/381 (27.82%), Postives = 162/381 (42.52%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADW+ L  DLL+LI  ++    + + FR+VC +WRS VP           CL I  +  
Sbjct: 1   MADWSLLPNDLLELIVGHLETSFEIVLFRSVCRSWRSVVPP-----LDHSRCLGIKTHDI 60

Query: 61  SSCGLFIFSD---NRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMT-----K 120
           S    F FSD   ++  F    + +           W    D+     L+  M      +
Sbjct: 61  SFNAGFTFSDQPTDQPPFKSSVDCTLKNIPIYLVKFWTPYGDD----YLIAEMREREGGE 120

Query: 121 ATLRLPPLSTFP-------NVVSFDSSRS-----GREYEVREFAGQIRRYSLRKMRDYFV 180
             L L PLS+         N V F+S  S     G+ YE+  F  Q  RY    +  +  
Sbjct: 121 PKLLLSPLSSNRIIYGMGINKVLFNSLTSPIIPFGQYYEITYFKKQPIRYRY-GLPYHLW 180

Query: 181 NKI----------VLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCED 240
           N++           L  +  +   F  +   R C NL  YRS  + SW+ V + P   E 
Sbjct: 181 NEVEWVETSERVEFLRLHSEDSRDFAVLFAGRMC-NLVMYRS-RNMSWTQVVEHP---ET 240

Query: 241 AIYSDC---DGVFYAVDC--YGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVRSGDEL 300
             Y D     G FYA+D    G V V++L     ++  +  G +Y   +K  +V+ G+EL
Sbjct: 241 YAYQDLVAFKGKFYALDSSGRGRVFVVELSLRVTEIPSV-KGSQYC--SKESLVQLGEEL 300

Query: 301 LLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFRMEWEG--PEWEKIESLNGMALFLGK 345
           LLV R++     R  +E +           F+VFR++ EG   +W ++  LN   +FLG 
Sbjct: 301 LLVQRFIP--AGRRYDEYI--------YTWFKVFRLDEEGGKRKWVQVNYLNDRVIFLGA 353

BLAST of MS021818 vs. ExPASy Swiss-Prot
Match: Q9FLV8 (Putative F-box/kelch-repeat protein At5g24040 OS=Arabidopsis thaliana OX=3702 GN=At5g24040 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.8e-11
Identity = 84/352 (23.86%), Postives = 146/352 (41.48%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPP-QLPCLLIPRYH 60
           M  W+EL P++L LIS  I    D I FR+VC  WRS       H+   + P  L P   
Sbjct: 1   MVKWSELPPEILHLISLKIDNPFDLIHFRSVCSFWRSSSLLKFRHMTSLRCPLPLDPGGC 60

Query: 61  CSSCGLFIFSDNRIHFLHLPEASHGK----RRCGSSHGWLAILDETPTILLLNPMTKATL 120
              C +     +R++ L  P     +    +     +G + +     ++ L    +    
Sbjct: 61  GDDCHIL---SSRVYLLKCPNRDRPQYWMFKLQAKENGEVVL----HSLFLRRNSSAYGC 120

Query: 121 RLPPLS-TFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNG 180
             P LS    N   F+ ++          A     + L +       +I        +N 
Sbjct: 121 LYPSLSIDLLNCQIFELAQ-------EHVACYSEWFELFECISKCEERIGFMGLNREKNE 180

Query: 181 FTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDL 240
           +  +  L   G LA YRS  D  W+ ++  P S  + I     G FYA+D  G  +V+  
Sbjct: 181 YMILGKLSFNG-LAMYRS-VDNRWTELEITPDSFFEGIV-PFKGKFYAIDRTGKTTVV-- 240

Query: 241 RDSRPKVSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTL 300
            D   +V+     R      KR+++ +GD+L+LV     +M ++   +  + P +R + +
Sbjct: 241 -DPTLEVNTFQRSRPCDKTRKRWLLMTGDKLILV-----EMCTKSRYD-FHIPNIREKKI 300

Query: 301 RFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTD 347
            FE+  +  E  +W+++E ++G  LFL    + +  A + P    N I + D
Sbjct: 301 WFEISELNEERNDWDQVEDMDGRVLFLEHYCTFSCLATEIPGFRANSIIFMD 326

BLAST of MS021818 vs. ExPASy Swiss-Prot
Match: Q9LVG8 (Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.7e-09
Identity = 85/368 (23.10%), Postives = 153/368 (41.58%), Query Frame = 0

Query: 2   ADWTELSPDLLQLISQNI------TVYQDYIRFRAVCHNWRSPVP-----ENPSHLPPQL 61
           + W++L  D+L+LIS  +      T++   +  R+VC  WR  +P        S  P  L
Sbjct: 9   SQWSDLPLDILELISDRLDHDSSDTIH--LLCLRSVCATWRLSLPLSNKNNRLSKFPKYL 68

Query: 62  PCLLIPRYHCSSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTIL-LLNP 121
           P         SS G F    + ++ L   EA    R C      + + + TP I+ +L+ 
Sbjct: 69  P---FWSSSSSSSGFFTLKQSNVYKL---EAPLNPRTC-----LVKLQETTPGIMRVLDL 128

Query: 122 MTKATLRLPPLSTFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRD----------YF 181
            +   +   P   FP+ +        +E+ VR      R Y +    +            
Sbjct: 129 FSNDRICFLP-ENFPSKIDL------QEFHVRLVR---RTYRMEYANNGGGAVPCFWSLH 188

Query: 182 VNKIVLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGV 241
            +K+V+ S+  +    +AI  +   G L F +SG D  W  +D+  +   + I    D  
Sbjct: 189 SDKVVILSSGED----SAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIMLYKDNR 248

Query: 242 FYAVDCYGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVR-SGDELLLVIRYLDDMFSR 301
              VD  G   + D+     KVS +A G       K+++V  SG E+ LV +Y+  ++ +
Sbjct: 249 CIVVDDKGKTVIYDVDF---KVSDLAEGLVGGGGHKKHLVECSGGEVFLVDKYVKTVWCK 308

Query: 302 VMNEGLNCPFMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEIS 347
                     +    + F V+ ++ E   WE++  L  +ALF+G + S +       +++
Sbjct: 309 --------SDISKSVVEFRVYNLKREEKRWEEVRDLGDVALFIGDDCSFSVQ-NPAGDLA 337

BLAST of MS021818 vs. ExPASy Swiss-Prot
Match: Q9ZUF1 (F-box protein At2g05970 OS=Arabidopsis thaliana OX=3702 GN=At2g05970 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.2e-08
Identity = 100/422 (23.70%), Postives = 157/422 (37.20%), Query Frame = 0

Query: 2   ADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPP---QLPCL-LIPR 61
           A W+EL PD+L+ + + ++ + D  R R+VC +W S       H  P   Q+P L L PR
Sbjct: 8   ASWSELCPDVLRCVFELLS-FSDLNRTRSVCSSWHSA----SRHCVPTQNQIPWLILFPR 67

Query: 62  YHC-----SSCGLFIFSDNRIHFLHLPEASHG--KRRCGSSHG-WLAILDETPTILLLNP 121
            +      +SC LF+  D+R       +   G     C +++G W+ ++D    + +LNP
Sbjct: 68  NNVNNNNNNSCVLFV-PDDRDSLYKTKDLGVGFMLSNCLATYGSWILMMDRLCNLNILNP 127

Query: 122 MTKATLRLPPLSTFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNP 181
           +T   + LP          FD  R                +   K +DY V         
Sbjct: 128 LTGEKIDLPR-------TKFDLPRLESSVAC--------LWIDEKTKDYIV--------- 187

Query: 182 SNQNGFTAIAILRRCGNLAFYRSGGDGSWS--FVDDGPSSCEDAIYSDCDGVFYAVDCYG 241
                      + +  N   Y   G+ +W   F  +   S E  +Y       Y      
Sbjct: 188 -----------VWKIKNSLVYAKKGNHTWQQVFSMNEELSVEQIVYEHKTQKLYVHFNDS 247

Query: 242 VVSVIDLRDSRPKVSRIATGRRYI----CNTKRYVVRSGDELLLVIRYLDDMFSRVMNEG 301
            +S+  L    P          YI            R  DE L V  Y+D   +  +   
Sbjct: 248 TLSIWRLSREDPH----GVFENYIPFDFVFQDFLPDRRTDEELYVKEYIDTRLNIALTTS 307

Query: 302 -----LNCPFMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEIS 361
                +     +S+   F +++M +    WE+IESL   AL L  +  I   A D P + 
Sbjct: 308 GELLKVASVVQKSKRWLFRIYKMNYIKRRWERIESLGDEALIL--DMGITIVAKDIPGLK 367

Query: 362 ENCIYYTD-DYCSETYIGECQRLDVGIFRLSDENFEPVATAWNSHS--SSSSIMRPSLSL 398
            N IY +  DY      G      + IF L+ +  EP+    +S +  S +    PS S 
Sbjct: 368 RNSIYISGFDY------GRKHLDHIFIFDLTTQESEPLPYCVSSSNDFSDARWFFPSFSQ 376

BLAST of MS021818 vs. ExPASy TrEMBL
Match: A0A5A7VGI0 (F-box protein SKIP23-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00870 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 2.0e-140
Identity = 263/407 (64.62%), Postives = 307/407 (75.43%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWTEL PDLLQ IS  +TVY DY+RFR VC +WRS VP+ P  LPPQLPCL+IP YH 
Sbjct: 1   MADWTELPPDLLQQISDYLTVYSDYLRFRVVCQSWRSSVPKIPHRLPPQLPCLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
             CGLF FSDN+IHFL+LPEAS  KR CGSSHGWL ILDETP+ILLLNP+T+A  RLPPL
Sbjct: 61  CRCGLFNFSDNKIHFLYLPEASLRKRPCGSSHGWLTILDETPSILLLNPLTRAKFRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYTRNLRQMRDSFVKKIVLSSSPSNQNDFLAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG GSW+FVDD PS CED IY+  DGVFYAVD YGVVS++DLR SR +
Sbjct: 181 LNHSGDLAFCRSGG-GSWTFVDDAPSDCEDVIYT--DGVFYAVDKYGVVSLMDLRGSRSQ 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPF--MRSRTLRFEV 300
           VS +AT  R +    +Y+VR G ELLLV RYLD     ++N+G++     +  RT+RFEV
Sbjct: 241 VSLVAT-ERQLAGDIQYLVRLGQELLLVSRYLD-----IVNDGMDDELISVMYRTVRFEV 300

Query: 301 FRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQ 360
           FRMEWEGP WEK+E+LN MALF+G NSS+AFSAADF EIS NCIYYTDDY    Y G+ +
Sbjct: 301 FRMEWEGPRWEKVENLNEMALFVGGNSSMAFSAADFGEISGNCIYYTDDYSDSDYQGQGE 360

Query: 361 RLDVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
             D+GIFRL DE+FEP+      + S  S  R  L LPPIW++PNPC
Sbjct: 361 EPDMGIFRLCDESFEPL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. ExPASy TrEMBL
Match: E5GCD1 (Ubiquitin-protein ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 2.0e-140
Identity = 263/407 (64.62%), Postives = 307/407 (75.43%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWTEL PDLLQ IS  +TVY DY+RFR VC +WRS VP+ P  LPPQLPCL+IP YH 
Sbjct: 1   MADWTELPPDLLQQISDYLTVYSDYLRFRVVCQSWRSSVPKIPHRLPPQLPCLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
             CGLF FSDN+IHFL+LPEAS  KR CGSSHGWL ILDETP+ILLLNP+T+A  RLPPL
Sbjct: 61  CRCGLFNFSDNKIHFLYLPEASLRKRPCGSSHGWLTILDETPSILLLNPLTRAKFRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYTRNLRQMRDSFVKKIVLSSSPSNQNDFLAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG GSW+FVDD PS CED IY+  DGVFYAVD YGVVS++DLR SR +
Sbjct: 181 LNHSGDLAFCRSGG-GSWTFVDDAPSDCEDVIYT--DGVFYAVDKYGVVSLMDLRGSRSQ 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPF--MRSRTLRFEV 300
           VS +AT  R +    +Y+VR G ELLLV RYLD     ++N+G++     +  RT+RFEV
Sbjct: 241 VSLVAT-ERQLAGDIQYLVRLGQELLLVSRYLD-----IVNDGMDDELISVMYRTVRFEV 300

Query: 301 FRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQ 360
           FRMEWEGP WEK+E+LN MALF+G NSS+AFSAADF EIS NCIYYTDDY    Y G+ +
Sbjct: 301 FRMEWEGPRWEKVENLNEMALFVGGNSSMAFSAADFGEISGNCIYYTDDYSDSDYQGQGE 360

Query: 361 RLDVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
             D+GIFRL DE+FEP+      + S  S  R  L LPPIW++PNPC
Sbjct: 361 EPDMGIFRLCDESFEPL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. ExPASy TrEMBL
Match: A0A1S3BVB7 (F-box protein SKIP23-like OS=Cucumis melo OX=3656 GN=LOC103493905 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 2.0e-140
Identity = 263/407 (64.62%), Postives = 307/407 (75.43%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWTEL PDLLQ IS  +TVY DY+RFR VC +WRS VP+ P  LPPQLPCL+IP YH 
Sbjct: 1   MADWTELPPDLLQQISDYLTVYSDYLRFRVVCQSWRSSVPKIPHRLPPQLPCLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
             CGLF FSDN+IHFL+LPEAS  KR CGSSHGWL ILDETP+ILLLNP+T+A  RLPPL
Sbjct: 61  CRCGLFNFSDNKIHFLYLPEASLRKRPCGSSHGWLTILDETPSILLLNPLTRAKFRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYTRNLRQMRDSFVKKIVLSSSPSNQNDFLAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG GSW+FVDD PS CED IY+  DGVFYAVD YGVVS++DLR SR +
Sbjct: 181 LNHSGDLAFCRSGG-GSWTFVDDAPSDCEDVIYT--DGVFYAVDKYGVVSLMDLRGSRSQ 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPF--MRSRTLRFEV 300
           VS +AT  R +    +Y+VR G ELLLV RYLD     ++N+G++     +  RT+RFEV
Sbjct: 241 VSLVAT-ERQLAGDIQYLVRLGQELLLVSRYLD-----IVNDGMDDELISVMYRTVRFEV 300

Query: 301 FRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQ 360
           FRMEWEGP WEK+E+LN MALF+G NSS+AFSAADF EIS NCIYYTDDY    Y G+ +
Sbjct: 301 FRMEWEGPRWEKVENLNEMALFVGGNSSMAFSAADFGEISGNCIYYTDDYSDSDYQGQGE 360

Query: 361 RLDVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
             D+GIFRL DE+FEP+      + S  S  R  L LPPIW++PNPC
Sbjct: 361 EPDMGIFRLCDESFEPL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. ExPASy TrEMBL
Match: A0A6J1ID46 (F-box protein SKIP23-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111474793 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 4.7e-137
Identity = 254/405 (62.72%), Postives = 307/405 (75.80%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L PDLLQ+IS N+ VY DY+RFR VC +WRS VP+ P  LPPQLP L+IP YH 
Sbjct: 1   MADWTQLPPDLLQVISDNLNVYSDYLRFRVVCQSWRSSVPKIPRRLPPQLPWLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF FSDN+IHFLHLPEAS G+RRCGSSHGWL ILDETPTILLLNP+T+A  RLPPL
Sbjct: 61  SRCGLFNFSDNKIHFLHLPEASLGRRRCGSSHGWLVILDETPTILLLNPLTRAKCRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD FV KIVLSS+PSNQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRIGREYLIRTPTGHIYSRNLRQMRDTFVKKIVLSSSPSNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG+ SW+FVDD PS CED +YS  DG+FYAVD +GV+SV+DLR SR +
Sbjct: 181 LNHSGDLAFCRSGGE-SWTFVDDAPSDCEDIVYS--DGLFYAVDKHGVISVMDLRGSRSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFR 300
           VS + TG +      +Y+V+ G ELLLV RYLD +   +++E ++  +   RT+RFEVFR
Sbjct: 241 VSLVTTGSQ-SAGDIQYLVKLGRELLLVSRYLDIINDAMVDELISVIY---RTVRFEVFR 300

Query: 301 MEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQRL 360
           MEWEGP WEK+E+L+ MALF+G NSS+AFSA+DF  IS NCIYYTDDY    Y G+ +  
Sbjct: 301 MEWEGPRWEKVENLDEMALFVGGNSSMAFSASDFGGISGNCIYYTDDYSDNDYQGQGEEP 360

Query: 361 DVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
           D+GIFRL D +FE +      + S  S  R  L LPPIW++PNPC
Sbjct: 361 DMGIFRLCDGSFEQL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. ExPASy TrEMBL
Match: A0A6J1F731 (F-box protein SKIP23-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442958 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 3.0e-136
Identity = 252/405 (62.22%), Postives = 308/405 (76.05%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADWT+L PDLLQ+I+ N+TVY DY+RFR VC +WRS VP+ P  LPPQLP L+IP YH 
Sbjct: 1   MADWTQLPPDLLQVIADNLTVYSDYLRFRVVCQSWRSSVPKIPRRLPPQLPWLIIPLYHD 60

Query: 61  SSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMTKATLRLPPL 120
           S CGLF FSDN+IHFLHLPEAS GKRRCGSSHGWLAI+DE PTILLLNP+T+A LRLPPL
Sbjct: 61  SRCGLFNFSDNKIHFLHLPEASLGKRRCGSSHGWLAIVDENPTILLLNPLTRAKLRLPPL 120

Query: 121 STFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNGFTAIAI 180
           STFPNVVSFD SR GREY +R   G I   +LR+MRD F+ KIVLSS+PSNQN F+A+AI
Sbjct: 121 STFPNVVSFDYSRVGREYLIRTPTGHIYSRNLRQMRDSFLKKIVLSSSPSNQNDFSAVAI 180

Query: 181 LRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDLRDSRPK 240
           L   G+LAF RSGG+ SW+FV+D PS CED +YS  DG+FYAVD +GV+SV+DLR S  +
Sbjct: 181 LNHSGDLAFCRSGGE-SWTFVNDAPSDCEDIVYS--DGLFYAVDKHGVISVMDLRGSHSE 240

Query: 241 VSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFR 300
           VS + TG +      +Y+V+ G ELLLV RYLD +   +++E ++  +   RT+RFEVFR
Sbjct: 241 VSLVTTGSQ-SAGDIQYLVKLGRELLLVSRYLDIINDAMVDELISVIY---RTVRFEVFR 300

Query: 301 MEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTDDYCSETYIGECQRL 360
           MEWEGP WEK+E+L+ MALF+G NSS+AFSA+DF  IS NCIYYTDDY    Y G+ +  
Sbjct: 301 MEWEGPRWEKVENLDEMALFVGGNSSMAFSASDFGGISGNCIYYTDDYSDNDYQGQGEEP 360

Query: 361 DVGIFRLSDENFEPVATAWNSHSSSSSIMRPSLSLPPIWISPNPC 406
           D+GIFRL D +FE +      + S  S  R  L LPPIW++PNPC
Sbjct: 361 DMGIFRLCDGSFEQL-----PYYSGGSHSRRRL-LPPIWVTPNPC 392

BLAST of MS021818 vs. TAIR 10
Match: AT2G17030.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-25
Identity = 110/400 (27.50%), Postives = 179/400 (44.75%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRS---PVPENPSHLPPQLPCLLIPR 60
           M DW+ L  DLL LIS+++    D I+FR+VC +WRS   P    P+H  P LP      
Sbjct: 1   MVDWSTLPKDLLDLISKSLESSFDLIQFRSVCSSWRSAAEPKSPLPTHHLPILP------ 60

Query: 61  YHCSSCGLFIFSDNRIHFLHLPEAS------HGKRRCGSSHGWLAILDE----TPTILLL 120
                 G  +F D+ + F  L + S      H       S GWL  ++E       + LL
Sbjct: 61  ----DNGGSLFPDSAVGF-RLSQRSILLIKPHEPCIESDSFGWLIKVEEDLNVPRKVTLL 120

Query: 121 NPMTKATLRLPPLSTFPNVV---SFDSSRSGREYEVREF--AGQIRRYSLRKMRDYFVNK 180
           +P+      +P    FP V+    F     GRE+++  F   G I       +   ++ K
Sbjct: 121 DPLCDTRNSIP--ENFPRVLDMSKFKVRELGREFKLHYFNTVGDI-------VESLYLEK 180

Query: 181 IVLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYA 240
            V+     + +    +  +   G LA +RS  D +W+ ++D PS  +D +    DG F+A
Sbjct: 181 AVVKYLDCDGDYKFVLLTIHVSGKLAVFRS-WDRAWTVINDMPSPYDDVML--FDGRFFA 240

Query: 241 VDCYGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVRSGDELLLVIRYLD----DMFSR 300
           VD  G   V+D   S  K++ +A+        K++++ S  E+LLV  YL     +    
Sbjct: 241 VDNNGRTVVVDY--SSLKLTLVAS--PVFGGDKKFLIESCGEMLLVDMYLSLEAVEGDPG 300

Query: 301 VMNEGLNCP--FMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAAD-FP 360
            + E    P  +M  RT++F+V+R       W  +  L    LFLG +S+ + SA+D  P
Sbjct: 301 FVEEIFEHPAFYMNERTVKFKVYRFVEREESWVDVYDLEDKMLFLGDDSTFSASASDILP 360

Query: 361 EISENCIYYTDDYCSETYIGECQRLDVGIFRLSDENFEPV 376
               + +++  +  +   +G  Q  D+G+F       E V
Sbjct: 361 LCDGSSVFFNGNVFNGEDLGAMQDRDLGVFDFRSGKIELV 373

BLAST of MS021818 vs. TAIR 10
Match: AT2G16290.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 76.6 bits (187), Expect = 5.0e-14
Identity = 106/381 (27.82%), Postives = 162/381 (42.52%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPPQLPCLLIPRYHC 60
           MADW+ L  DLL+LI  ++    + + FR+VC +WRS VP           CL I  +  
Sbjct: 1   MADWSLLPNDLLELIVGHLETSFEIVLFRSVCRSWRSVVPP-----LDHSRCLGIKTHDI 60

Query: 61  SSCGLFIFSD---NRIHFLHLPEASHGKRRCGSSHGWLAILDETPTILLLNPMT-----K 120
           S    F FSD   ++  F    + +           W    D+     L+  M      +
Sbjct: 61  SFNAGFTFSDQPTDQPPFKSSVDCTLKNIPIYLVKFWTPYGDD----YLIAEMREREGGE 120

Query: 121 ATLRLPPLSTFP-------NVVSFDSSRS-----GREYEVREFAGQIRRYSLRKMRDYFV 180
             L L PLS+         N V F+S  S     G+ YE+  F  Q  RY    +  +  
Sbjct: 121 PKLLLSPLSSNRIIYGMGINKVLFNSLTSPIIPFGQYYEITYFKKQPIRYRY-GLPYHLW 180

Query: 181 NKI----------VLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCED 240
           N++           L  +  +   F  +   R C NL  YRS  + SW+ V + P   E 
Sbjct: 181 NEVEWVETSERVEFLRLHSEDSRDFAVLFAGRMC-NLVMYRS-RNMSWTQVVEHP---ET 240

Query: 241 AIYSDC---DGVFYAVDC--YGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVRSGDEL 300
             Y D     G FYA+D    G V V++L     ++  +  G +Y   +K  +V+ G+EL
Sbjct: 241 YAYQDLVAFKGKFYALDSSGRGRVFVVELSLRVTEIPSV-KGSQYC--SKESLVQLGEEL 300

Query: 301 LLVIRYLDDMFSRVMNEGLNCPFMRSRTLRFEVFRMEWEG--PEWEKIESLNGMALFLGK 345
           LLV R++     R  +E +           F+VFR++ EG   +W ++  LN   +FLG 
Sbjct: 301 LLVQRFIP--AGRRYDEYI--------YTWFKVFRLDEEGGKRKWVQVNYLNDRVIFLGA 353

BLAST of MS021818 vs. TAIR 10
Match: AT5G24040.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 70.9 bits (172), Expect = 2.7e-12
Identity = 84/352 (23.86%), Postives = 146/352 (41.48%), Query Frame = 0

Query: 1   MADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPP-QLPCLLIPRYH 60
           M  W+EL P++L LIS  I    D I FR+VC  WRS       H+   + P  L P   
Sbjct: 1   MVKWSELPPEILHLISLKIDNPFDLIHFRSVCSFWRSSSLLKFRHMTSLRCPLPLDPGGC 60

Query: 61  CSSCGLFIFSDNRIHFLHLPEASHGK----RRCGSSHGWLAILDETPTILLLNPMTKATL 120
              C +     +R++ L  P     +    +     +G + +     ++ L    +    
Sbjct: 61  GDDCHIL---SSRVYLLKCPNRDRPQYWMFKLQAKENGEVVL----HSLFLRRNSSAYGC 120

Query: 121 RLPPLS-TFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNPSNQNG 180
             P LS    N   F+ ++          A     + L +       +I        +N 
Sbjct: 121 LYPSLSIDLLNCQIFELAQ-------EHVACYSEWFELFECISKCEERIGFMGLNREKNE 180

Query: 181 FTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGVFYAVDCYGVVSVIDL 240
           +  +  L   G LA YRS  D  W+ ++  P S  + I     G FYA+D  G  +V+  
Sbjct: 181 YMILGKLSFNG-LAMYRS-VDNRWTELEITPDSFFEGIV-PFKGKFYAIDRTGKTTVV-- 240

Query: 241 RDSRPKVSRIATGRRYICNTKRYVVRSGDELLLVIRYLDDMFSRVMNEGLNCPFMRSRTL 300
            D   +V+     R      KR+++ +GD+L+LV     +M ++   +  + P +R + +
Sbjct: 241 -DPTLEVNTFQRSRPCDKTRKRWLLMTGDKLILV-----EMCTKSRYD-FHIPNIREKKI 300

Query: 301 RFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEISENCIYYTD 347
            FE+  +  E  +W+++E ++G  LFL    + +  A + P    N I + D
Sbjct: 301 WFEISELNEERNDWDQVEDMDGRVLFLEHYCTFSCLATEIPGFRANSIIFMD 326

BLAST of MS021818 vs. TAIR 10
Match: AT5G60060.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 63.9 bits (154), Expect = 3.3e-10
Identity = 85/368 (23.10%), Postives = 153/368 (41.58%), Query Frame = 0

Query: 2   ADWTELSPDLLQLISQNI------TVYQDYIRFRAVCHNWRSPVP-----ENPSHLPPQL 61
           + W++L  D+L+LIS  +      T++   +  R+VC  WR  +P        S  P  L
Sbjct: 9   SQWSDLPLDILELISDRLDHDSSDTIH--LLCLRSVCATWRLSLPLSNKNNRLSKFPKYL 68

Query: 62  PCLLIPRYHCSSCGLFIFSDNRIHFLHLPEASHGKRRCGSSHGWLAILDETPTIL-LLNP 121
           P         SS G F    + ++ L   EA    R C      + + + TP I+ +L+ 
Sbjct: 69  P---FWSSSSSSSGFFTLKQSNVYKL---EAPLNPRTC-----LVKLQETTPGIMRVLDL 128

Query: 122 MTKATLRLPPLSTFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRD----------YF 181
            +   +   P   FP+ +        +E+ VR      R Y +    +            
Sbjct: 129 FSNDRICFLP-ENFPSKIDL------QEFHVRLVR---RTYRMEYANNGGGAVPCFWSLH 188

Query: 182 VNKIVLSSNPSNQNGFTAIAILRRCGNLAFYRSGGDGSWSFVDDGPSSCEDAIYSDCDGV 241
            +K+V+ S+  +    +AI  +   G L F +SG D  W  +D+  +   + I    D  
Sbjct: 189 SDKVVILSSGED----SAIIAIHSGGKLGFLKSGNDEKWKILDNSWNVIYEDIMLYKDNR 248

Query: 242 FYAVDCYGVVSVIDLRDSRPKVSRIATGRRYICNTKRYVVR-SGDELLLVIRYLDDMFSR 301
              VD  G   + D+     KVS +A G       K+++V  SG E+ LV +Y+  ++ +
Sbjct: 249 CIVVDDKGKTVIYDVDF---KVSDLAEGLVGGGGHKKHLVECSGGEVFLVDKYVKTVWCK 308

Query: 302 VMNEGLNCPFMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEIS 347
                     +    + F V+ ++ E   WE++  L  +ALF+G + S +       +++
Sbjct: 309 --------SDISKSVVEFRVYNLKREEKRWEEVRDLGDVALFIGDDCSFSVQ-NPAGDLA 337

BLAST of MS021818 vs. TAIR 10
Match: AT2G05970.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 60.5 bits (145), Expect = 3.7e-09
Identity = 100/422 (23.70%), Postives = 157/422 (37.20%), Query Frame = 0

Query: 2   ADWTELSPDLLQLISQNITVYQDYIRFRAVCHNWRSPVPENPSHLPP---QLPCL-LIPR 61
           A W+EL PD+L+ + + ++ + D  R R+VC +W S       H  P   Q+P L L PR
Sbjct: 8   ASWSELCPDVLRCVFELLS-FSDLNRTRSVCSSWHSA----SRHCVPTQNQIPWLILFPR 67

Query: 62  YHC-----SSCGLFIFSDNRIHFLHLPEASHG--KRRCGSSHG-WLAILDETPTILLLNP 121
            +      +SC LF+  D+R       +   G     C +++G W+ ++D    + +LNP
Sbjct: 68  NNVNNNNNNSCVLFV-PDDRDSLYKTKDLGVGFMLSNCLATYGSWILMMDRLCNLNILNP 127

Query: 122 MTKATLRLPPLSTFPNVVSFDSSRSGREYEVREFAGQIRRYSLRKMRDYFVNKIVLSSNP 181
           +T   + LP          FD  R                +   K +DY V         
Sbjct: 128 LTGEKIDLPR-------TKFDLPRLESSVAC--------LWIDEKTKDYIV--------- 187

Query: 182 SNQNGFTAIAILRRCGNLAFYRSGGDGSWS--FVDDGPSSCEDAIYSDCDGVFYAVDCYG 241
                      + +  N   Y   G+ +W   F  +   S E  +Y       Y      
Sbjct: 188 -----------VWKIKNSLVYAKKGNHTWQQVFSMNEELSVEQIVYEHKTQKLYVHFNDS 247

Query: 242 VVSVIDLRDSRPKVSRIATGRRYI----CNTKRYVVRSGDELLLVIRYLDDMFSRVMNEG 301
            +S+  L    P          YI            R  DE L V  Y+D   +  +   
Sbjct: 248 TLSIWRLSREDPH----GVFENYIPFDFVFQDFLPDRRTDEELYVKEYIDTRLNIALTTS 307

Query: 302 -----LNCPFMRSRTLRFEVFRMEWEGPEWEKIESLNGMALFLGKNSSIAFSAADFPEIS 361
                +     +S+   F +++M +    WE+IESL   AL L  +  I   A D P + 
Sbjct: 308 GELLKVASVVQKSKRWLFRIYKMNYIKRRWERIESLGDEALIL--DMGITIVAKDIPGLK 367

Query: 362 ENCIYYTD-DYCSETYIGECQRLDVGIFRLSDENFEPVATAWNSHS--SSSSIMRPSLSL 398
            N IY +  DY      G      + IF L+ +  EP+    +S +  S +    PS S 
Sbjct: 368 RNSIYISGFDY------GRKHLDHIFIFDLTTQESEPLPYCVSSSNDFSDARWFFPSFSQ 376