MS021816 (gene) Bitter gourd (TR) v1

Overview


Name	MS021816
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	tRNA pseudouridine synthase family protein
Location	scaffold1: 531200 .. 532836 (+)
RNA-Seq Expression	MS021816
Synteny	MS021816

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGAAGAAAAACCGCCAACGGAAACCAAAAGATCACCGCCACAACCGTCGATTGAAGAACCAGATCCGAAGAAGCTCAAAATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAAACGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAAAGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGGCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGTGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGTAAGTAAATCACCACTAAATTTTGACTTGATTCTGCCTTTGTTAATAGAATTGGATTTAATCAACTCTTAGTAATCAAACTTCAAACACAGGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGATATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATT

mRNA sequence

GAAGAAGAAAAACCGCCAACGGAAACCAAAAGATCACCGCCACAACCGTCGATTGAAGAACCAGATCCGAAGAAGCTCAAAATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAAACGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAAAGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGGCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGTGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGATATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATT

Coding sequence (CDS)

GAAGAAGAAAAACCGCCAACGGAAACCAAAAGATCACCGCCACAACCGTCGATTGAAGAACCAGATCCGAAGAAGCTCAAAATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAAACGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAAAGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGGCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGTGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGATATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATT

Protein sequence

EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI

Homology

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BLAST of MS021816 vs. NCBI nr
Match: XP_022132914.1 (tRNA pseudouridine synthase A, mitochondrial-like [Momordica charantia])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.1e-289
Identity = 511/515 (99.22%), Postives = 512/515 (99.42%), Query Frame = 0


Query: 1   EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF 60
           EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF
Sbjct: 2   EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF 61

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV
Sbjct: 62  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV 121

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN
Sbjct: 122 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN 181

Query: 181 PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNA 240
           PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTG A
Sbjct: 182 PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGIA 241

Query: 241 VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSK 300
           VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVK ECNETSGLGNDSKTVVSK
Sbjct: 242 VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSK 301

Query: 301 ESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA 360
           ES DGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA
Sbjct: 302 ESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA 361

Query: 361 TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 420
           TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA
Sbjct: 362 TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 421

Query: 421 PEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW 480
           PEVGLYLEECLFTSYNQKWKESHEE+SMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW
Sbjct: 422 PEVGLYLEECLFTSYNQKWKESHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW 481

Query: 481 LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI 516
           LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI
Sbjct: 482 LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI 516

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BLAST of MS021816 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo] >KAA0064620.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa] >TYK19972.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 901.0 bits (2327), Expect = 4.8e-258
Identity = 462/517 (89.36%), Postives = 482/517 (93.23%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+RSPPQP IEEPDPKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFE KT ITES++SSN  NA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    G L D+   SDSNKQTSVLVEN  TEL + SEG  KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +G EKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK++HEE+SMKAYEEVAEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRV DEVA  A +T V MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

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BLAST of MS021816 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 894.0 bits (2309), Expect = 5.9e-256
Identity = 457/517 (88.39%), Postives = 481/517 (93.04%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPT+T+RSPPQPSIEEP+PKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRKIAIFF
Sbjct: 2   EEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFE KT I+ES++S N GNA+
Sbjct: 182 NCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    GAL DN  ASDS K+TSVLVEN  TELN+GSE   KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSENV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSK+S + NEKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARRFI+SF
Sbjct: 302 VSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIISF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DAA TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNINV
Sbjct: 362 DAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK+SHEE+SMKAYEEVAEDFKMK IYSHI STEHKEGAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRVADE AD A +T V +ET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAE 518

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BLAST of MS021816 vs. NCBI nr
Match: XP_004145528.1 (tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_012098 [Cucumis sativus])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 457/517 (88.39%), Postives = 480/517 (92.84%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+RSPPQP IEEP PKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFE KT ITES++SSNT NA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    G L D+   SDSNKQTSVLVEN  TELN+GSEGT KSE +E SGLG+DS+ V
Sbjct: 242 EVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +G EK SGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK++HEE+SMKAYEE AEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRV DEVA  A +  + MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAE 518

[Close]

BLAST of MS021816 vs. NCBI nr
Match: XP_038899088.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 891.0 bits (2301), Expect = 5.0e-255
Identity = 459/517 (88.78%), Postives = 480/517 (92.84%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+ SPPQP IEEPDPKKLKMSSTTTTSDDEDVN +SAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRISPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSSSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFE KT I ES +SSNTGNA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAIIESHISSNTGNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+      L DN   SDSNKQ+SVLVEN  TELN+ SE T KS  +E SGLGNDS+ V
Sbjct: 242 EVSDVQENRVLEDNATISDSNKQSSVLVENSCTELNVESESTAKSVGSEMSGLGNDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +GNEKPSGSFKY EEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGNEKPSGSFKYCEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK+SHEE+SMKAYEEVAEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLR  DEVA+ A +T V MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRAVDEVAEIAMNTSVAMETQAE 518

[Close]

BLAST of MS021816 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 4.2e-55
Identity = 158/480 (32.92%), Postives = 237/480 (49.38%), Query Frame = 0


Query: 7   TETKRSPPQPSIEEPDPKKLK------MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF 66
           T  ++S P PS  E D K+LK           T   DE+  P   AP+K    +RK+A+ 
Sbjct: 98  TRVRKSSPSPSANENDKKRLKKEFQPRQKIEYTPLVDENGQPIPKAPRK---PKRKVAVM 157

Query: 67  FAYCGVGYQGM--QKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVS 126
             YCG GY GM  Q +P  KTIE D+ +A+  AGA+   +    K+  + R+ARTDKGV 
Sbjct: 158 LGYCGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTDKGVH 217

Query: 127 AVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFA 186
           A G V+S +  I+ P  ++++N  L  QIRI+G +R T  F+ +K C  R Y YL+P F+
Sbjct: 218 AAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFS 277

Query: 187 LNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTV---ITESEVSS 246
           L P   +   SV + L  + +L +  +  E  +  EG+    N ++K +   IT+ ++ S
Sbjct: 278 LLPPKPK---SVLSELVKEKKL-ENPDLFEDDQ--EGIDWWENVKSKILASGITQEQIDS 337

Query: 247 NTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSK 306
            T +  E                +Q     +  D E    SE +      +   + N S+
Sbjct: 338 ITSSYDE-------------QQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQSR 397

Query: 307 TVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIV 366
                           S++        F  ++K Y GTHNFHNFT     +D +A RF++
Sbjct: 398 R---------------SYRISSSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSANRFMI 457

Query: 367 SFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNI 426
               +   V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R   P  +I    Q    I
Sbjct: 458 KTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIINNFFQ-STKI 517

Query: 427 NVPTAPEVGLYLEECLFTSYNQK-WKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKE 475
           N+P AP +GL LE  +F  YN K  K  +E I    +++   +FKMK IY  I + E KE
Sbjct: 518 NIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNEFKMKYIYDKIYAEESKE 539

[Close]

BLAST of MS021816 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 2.4e-50
Identity = 160/519 (30.83%), Postives = 223/519 (42.97%), Query Frame = 0


Query: 5   PPTETKRSPPQPSIEEPDPK-------KLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKI 64
           PP    + PP     +PD K       +            ED  P    PK      RKI
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPK------RKI 88

Query: 65  AIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTD 124
            +  AY G GY GMQ+N G+   +TIE DL  AL  AG +PE+     ++  + R ARTD
Sbjct: 89  VLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTD 148

Query: 125 KGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLI 184
           KGVSA GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++
Sbjct: 149 KGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYML 208

Query: 185 PVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVS 244
           P FA    +H+DR+                                              
Sbjct: 209 PTFAF---AHKDRD---------------------------------------------- 268

Query: 245 SNTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDS 304
                                                        V+ E           
Sbjct: 269 ---------------------------------------------VQDE----------- 328

Query: 305 KTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 364
                            S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I
Sbjct: 329 -----------------SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYI 388

Query: 365 VSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 424
           +        V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  
Sbjct: 389 LEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-K 417

Query: 425 INVPTAPEVGLYLEECLFTSYNQKWKES--HEEISMKAYEEVAEDFKMKQIYSHIASTEH 484
           ++VP AP +GL LE   F  YNQ++     HE +     E     FK + IY  I STE 
Sbjct: 449 VDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTER 417

Query: 485 KEGAVALWLHSLNHRNYPDLRV-ADEVADKANDTCVGME 511
            E ++A WL++L   N+    + AD+   K   +  G E
Sbjct: 509 DERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSE 417

[Close]

BLAST of MS021816 vs. ExPASy Swiss-Prot
Match: Q9Y606 (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 200.3 bits (508), Expect = 5.3e-50
Identity = 141/447 (31.54%), Postives = 201/447 (44.97%), Query Frame = 0


Query: 48  KKQRYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHDRGLSKR 107
           ++++  +RKI +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE+     ++
Sbjct: 77  RREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRK 136

Query: 108 YDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKF 167
             + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT  FN+K  
Sbjct: 137 MSFQRCARTDKGVSAAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVTGGFNSKNR 196

Query: 168 CDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEA 227
           CD R Y YL+P FA    +H+DR+                                    
Sbjct: 197 CDARTYCYLLPTFAF---AHKDRD------------------------------------ 256

Query: 228 KTVITESEVSSNTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSEC 287
                                                                  V+ E 
Sbjct: 257 -------------------------------------------------------VQDE- 316

Query: 288 NETSGLGNDSKTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTK 347
                                      +++   E   + NR+L  Y GTHNFHNFT++  
Sbjct: 317 ---------------------------TYRLSAETLQQVNRLLACYKGTHNFHNFTSQKG 376

Query: 348 AEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 407
            +DP+A R+I+        V EG+EF    V GQSFM+HQIRKM+GL VAI++  APES+
Sbjct: 377 PQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESV 399

Query: 408 IEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKES--HEEISMKAYEEVAEDFKMKQ 467
           +E++   +  ++VP AP +GL LE   F  YNQ++     HE +     E     FK + 
Sbjct: 437 LERSWGTE-KVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEH 399

Query: 468 IYSHIASTEHKEGAVALWLHSLNHRNY 490
           IY  I  TE  E ++A WL +L   N+
Sbjct: 497 IYPTIIGTERDERSMAQWLSTLPIHNF 399

[Close]

BLAST of MS021816 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-49
Identity = 155/497 (31.19%), Postives = 216/497 (43.46%), Query Frame = 0


Query: 5   PPTETKRSPPQPSIEEPDPK-------KLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKI 64
           PP    + PP  +  +PD K       +            ED  P    PK      RKI
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPK------RKI 88

Query: 65  AIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTD 124
            +  AY G GY GMQ+N G+   +TIE DL  AL  AG +PE+     ++  + R ARTD
Sbjct: 89  VLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTD 148

Query: 125 KGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLI 184
           KGVSA GQVVS + ++     +D++NS+L   IRI G KRVT  FN+K  CD R Y Y++
Sbjct: 149 KGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYML 208

Query: 185 PVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVS 244
           P FA    +H+DR+                                              
Sbjct: 209 PTFAF---AHKDRD---------------------------------------------- 268

Query: 245 SNTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDS 304
                                                        V+ E           
Sbjct: 269 ---------------------------------------------VQDE----------- 328

Query: 305 KTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFI 364
                            S++   E   + NR+L  Y GTHNFHNFT++    +P+ARR+I
Sbjct: 329 -----------------SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYI 388

Query: 365 VSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVN 424
           +        V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  
Sbjct: 389 LEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-K 395

Query: 425 INVPTAPEVGLYLEECLFTSYNQKWKES--HEEISMKAYEEVAEDFKMKQIYSHIASTEH 484
           ++VP AP +GL LE   F  YNQ++     HE +     E     FK + IY  I STE 
Sbjct: 449 VDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTER 395

Query: 485 KEGAVALWLHSLNHRNY 490
            E ++A WL++L   N+
Sbjct: 509 DERSMAQWLNTLPIHNF 395

[Close]

BLAST of MS021816 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.7e-49
Identity = 135/427 (31.62%), Postives = 207/427 (48.48%), Query Frame = 0


Query: 51  RYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARS 110
           R  +RK+A    YCG GY GMQ NP  KTIE D+ +A   AGA+ +++    K+  + R+
Sbjct: 81  RQPKRKVACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 140

Query: 111 ARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRY 170
           ARTDKGV A G V+S +  I+    V+++NS+L  Q+R++G  R   +F  +K C  R Y
Sbjct: 141 ARTDKGVHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVY 200

Query: 171 VYLIPVFAL---NPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTV 230
            YL+P ++     P +    + +K     D E                  GK+ +E+   
Sbjct: 201 EYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEE------------------GKKYWESVAA 260

Query: 231 ITESEVSSNTGNAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNET 290
             ES+  S                    D   + + + E    E    +E  VK++    
Sbjct: 261 DLESQGVS-------------------YDEWMKRACIDEIKGEETKEVAESEVKTDSKTD 320

Query: 291 SGLGNDSKTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAED 350
           +      K V  +   +        F+   E  A+   ILK Y GTHNFHNFT     +D
Sbjct: 321 AATLEKIKAVERRHREE--------FRISGERLAKIREILKIYEGTHNFHNFTLGKAFKD 380

Query: 351 PAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPESLIE 410
           P+A R + S   +   +++G E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+ LI 
Sbjct: 381 PSAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIP 440

Query: 411 KALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKE--SHEEISMKAYEEVAEDFKMKQIY 470
           +  +K   IN+P AP +GL LE  ++ SYN+K +     E +    + +  E FK K IY
Sbjct: 441 QTFEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIY 461

Query: 471 SHIASTE 472
             I + E
Sbjct: 501 DKIYAEE 461

[Close]

BLAST of MS021816 vs. ExPASy TrEMBL
Match: A0A6J1BUF3 (tRNA pseudouridine synthase A, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111005637 PE=3 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 2.0e-289
Identity = 511/515 (99.22%), Postives = 512/515 (99.42%), Query Frame = 0


Query: 1   EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF 60
           EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF
Sbjct: 2   EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIF 61

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV
Sbjct: 62  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAV 121

Query: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN 180
           GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN
Sbjct: 122 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALN 181

Query: 181 PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNA 240
           PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTG A
Sbjct: 182 PNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGIA 241

Query: 241 VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSK 300
           VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVK ECNETSGLGNDSKTVVSK
Sbjct: 242 VEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSK 301

Query: 301 ESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA 360
           ES DGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA
Sbjct: 302 ESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAA 361

Query: 361 TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 420
           TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA
Sbjct: 362 TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTA 421

Query: 421 PEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW 480
           PEVGLYLEECLFTSYNQKWKESHEE+SMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW
Sbjct: 422 PEVGLYLEECLFTSYNQKWKESHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALW 481

Query: 481 LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI 516
           LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI
Sbjct: 482 LHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI 516

[Close]

BLAST of MS021816 vs. ExPASy TrEMBL
Match: A0A1S3BV07 (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 462/517 (89.36%), Postives = 482/517 (93.23%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+RSPPQP IEEPDPKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFE KT ITES++SSN  NA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    G L D+   SDSNKQTSVLVEN  TEL + SEG  KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +G EKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK++HEE+SMKAYEEVAEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRV DEVA  A +T V MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of MS021816 vs. ExPASy TrEMBL
Match: A0A5A7VBT0 (tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00860 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 462/517 (89.36%), Postives = 482/517 (93.23%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+RSPPQP IEEPDPKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFE KT ITES++SSN  NA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    G L D+   SDSNKQTSVLVEN  TEL + SEG  KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +G EKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK++HEE+SMKAYEEVAEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRV DEVA  A +T V MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of MS021816 vs. ExPASy TrEMBL
Match: E5GCD2 (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 462/517 (89.36%), Postives = 482/517 (93.23%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPTET+RSPPQP IEEPDPKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFF
Sbjct: 2   EEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFE KT ITES++SSN  NA+
Sbjct: 182 NCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    G L D+   SDSNKQTSVLVEN  TEL + SEG  KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEIV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSKES +G EKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF
Sbjct: 302 VSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DA+TTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV
Sbjct: 362 DASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK++HEE+SMKAYEEVAEDFKMKQIYSHIASTEHK+GAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRV DEVA  A +T V MET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAE 518

[Close]

BLAST of MS021816 vs. ExPASy TrEMBL
Match: A0A6J1IJ76 (tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.9e-256
Identity = 457/517 (88.39%), Postives = 481/517 (93.04%), Query Frame = 0


Query: 2   EEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFF 61
           EE+PPT+T+RSPPQPSIEEP+PKKLKMSSTTTTSDDEDVN NSAAPKKQRYKRRKIAIFF
Sbjct: 2   EEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIFF 61

Query: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVG 121
           AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPE DRGLSKRYDWARSARTDKGVSAVG
Sbjct: 62  AYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVG 121

Query: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNP 181
           QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+P
Sbjct: 122 QVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDP 181

Query: 182 NSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGNAV 241
           N HRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFE KT I+ES++S N GNA+
Sbjct: 182 NCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNAI 241

Query: 242 EVSE----GALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTV 301
           EVS+    GAL DN  ASDS K+TSVLVEN  TELN+GSE   KSE +E SGLGNDS+ V
Sbjct: 242 EVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSENV 301

Query: 302 VSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSF 361
           VSK+S + NEKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAARRFI+SF
Sbjct: 302 VSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIISF 361

Query: 362 DAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINV 421
           DAA TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNINV
Sbjct: 362 DAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNINV 421

Query: 422 PTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAV 481
           PTAPEVGLYL+ECLFTSYNQKWK+SHEE+SMKAYEEVAEDFKMK IYSHI STEHKEGAV
Sbjct: 422 PTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGAV 481

Query: 482 ALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAE 515
           ALWLHSLNHRNYPDLRVADE AD A +T V +ET AE
Sbjct: 482 ALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAE 518

[Close]

BLAST of MS021816 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 535.4 bits (1378), Expect = 5.0e-152
Identity = 292/501 (58.28%), Postives = 367/501 (73.25%), Query Frame = 0


Query: 1   EEEKPPTETKRSPPQPSIEEPDPKKLKMSSTTTTSDDEDV---NPNSAAPKKQRYKRRKI 60
           E ++P  E       P   EPD KKLK+S+T   +D   V     N A  +  +Y+RRK+
Sbjct: 2   ENQEPDIEKLPRDDVPD-REPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKV 61

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGV 120
           AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGV
Sbjct: 62  AIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGV 121

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFY+DPPGFV+RLNS L  QIR+FGYKRV  SF++KKFCDRRRYVYLIPVF
Sbjct: 122 SAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVF 181

Query: 181 ALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVE-GLMGKRN--FEAKTVITESEVS 240
           AL+P  H + E V+  LG   E VKC+ECSE+G K+  G+MGK     + K++  +S++S
Sbjct: 182 ALDPCVHGEAEMVRTDLG--YEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDIS 241

Query: 241 SNTGNAV--EVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGN 300
           S+T +A+  +V    L  + P++++N  + VL     + L+  +EG  +S          
Sbjct: 242 SSTCDAIGADVKCETLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEES--------NT 301

Query: 301 DSKTVVSKESGDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARR 360
            +K  ++   G+   K    F YGE+EK RFNRIL +YVG+HNFHNFTTRTKA DPAA R
Sbjct: 302 LAKVEMNNGEGEDMTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANR 361

Query: 361 FIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKD 420
           +I+SF+A T + ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLIE + +KD
Sbjct: 362 YILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKD 421

Query: 421 VNINVPTAPEVGLYLEECLFTSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEH 480
           V INVP APEVGLYL+EC FTSYN+++K SHEE+SM+ Y+EVAE+FK K +YSHI S E 
Sbjct: 422 VRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGSAEE 481

Query: 481 KEGAVALWLHSLNHRNYPDLR 494
           K+GAVA+WLHSLN RNYPDLR
Sbjct: 482 KDGAVAIWLHSLNQRNYPDLR 491

[Close]

BLAST of MS021816 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.7e-147
Identity = 279/468 (59.62%), Postives = 337/468 (72.01%), Query Frame = 0


Query: 31  TTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFAL+P SHRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEAKTVITESEVSSNTGNAV--EVSEGALGDNTPASDSNKQTSVL 270
           RK+  GL+GK    NF  K++  +S++SSN  +A+  ++   AL  N     S       
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGR 242

Query: 271 VENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSKESGDGNEKPSGSFKYGEEEKARFN 330
           ++    +LN  S  T                            K    F YGE+EK RF+
Sbjct: 243 IQEDSCKLNTNSSET----------------------------KVKSKFCYGEKEKERFS 302

Query: 331 RILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQ 390
           RIL  YVG++NFHNFTTRTKA+DP A R I+SF A T + ++GI+F+KCEV+G+SFMLHQ
Sbjct: 303 RILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQ 362

Query: 391 IRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHE 450
           IRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYL+EC FTSYN+ +++SHE
Sbjct: 363 IRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDSHE 422

Query: 451 EISMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDL 493
           E+SM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 EVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

[Close]

BLAST of MS021816 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.7e-110
Identity = 221/387 (57.11%), Postives = 267/387 (68.99%), Query Frame = 0


Query: 31  TTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++ D++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFAL+P SHRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEAKTVITESEVSSNTGNAV--EVSEGALGDNTPASDSNKQTSVL 270
           RK+  GL+GK    NF  K++  +S++SSN  +A+  ++   AL  N     S       
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGR 242

Query: 271 VENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSKESGDGNEKPSGSFKYGEEEKARFN 330
           ++    +LN  S  T                            K    F YGE+EK RF+
Sbjct: 243 IQEDSCKLNTNSSET----------------------------KVKSKFCYGEKEKERFS 302

Query: 331 RILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQ 390
           RIL  YVG++NFHNFTTRTKA+DP A R I+SF A T + ++GI+F+KCEV+G+SFMLHQ
Sbjct: 303 RILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQ 356

Query: 391 IRKMMGLAVAIMRNCAPESLIEKALQK 412
           IRKMMGLAVAIMRNCA ESLI+ A  K
Sbjct: 363 IRKMMGLAVAIMRNCASESLIQSAFSK 356

[Close]

BLAST of MS021816 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-25
Identity = 114/426 (26.76%), Postives = 184/426 (43.19%), Query Frame = 0


Query: 23  PKKLKMSST-----TTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS     + N  A K + Y+++K+ I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHR 202
              DP G V    ++ +L   IR+F        F+ ++ C  R+Y YL+PV  L   +  
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSF 205

Query: 203 DRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVI---TESEVSSNTGNAVE 262
             + +   +   NE++K         + EG     N+  ++     +E ++    G    
Sbjct: 206 TSDEIDYHITDFNEILK---------EFEGEYPFHNYTQRSRYRRKSEQKIKQRNGRPPR 265

Query: 263 VSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKSECNETSGLGNDSKTVVSKES 322
             +     +  AS+S  +    +E G+ E              E    G   + VV+ +S
Sbjct: 266 EPK-----SIKASESEFREENHIEIGEEE-------------EEKEVDGESDEHVVTPDS 325

Query: 323 GDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRT-KAEDPAARRFIVSFDAAT 382
            +        + Y  +E  + +       G H    F  R+ K E+              
Sbjct: 326 DNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFRCRSGKLENSL------------ 385

Query: 383 TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAP 428
                G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P AP
Sbjct: 386 -----GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAP 400

[Close]

BLAST of MS021816 vs. TAIR 10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.5e-10
Identity = 32/61 (52.46%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 433 TSYNQKWKESHEEISMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDL 492
           TS    W+E   +      E+  + FK K +YSHI S E K+GAVA+WLHSLN RNYPDL
Sbjct: 77  TSARISWREVSVDDDQ---EQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDL 134

Query: 493 R 494
           R
Sbjct: 137 R 134

The following BLAST results are available for this feature:

BLAST of MS021816 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132914.1	4.1e-289	99.22	tRNA pseudouridine synthase A, mitochondrial-like [Momordica charantia]	[more]
XP_008452911.1	4.8e-258	89.36	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_022975443.1	5.9e-256	88.39	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
XP_004145528.1	1.7e-255	88.39	tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein...	[more]
XP_038899088.1	5.0e-255	88.78	tRNA pseudouridine synthase A [Benincasa hispida]	[more]

BLAST of MS021816 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q59S63	4.2e-55	32.92	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q4KM92	2.4e-50	30.83	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]
Q9Y606	5.3e-50	31.54	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]
Q9WU56	1.2e-49	31.19	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]
Q6CC39	2.7e-49	31.62	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]

BLAST of MS021816 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUF3	2.0e-289	99.22	tRNA pseudouridine synthase A, mitochondrial-like OS=Momordica charantia OX=3673...	[more]
A0A1S3BV07	2.3e-258	89.36	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7VBT0	2.3e-258	89.36	tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
E5GCD2	2.3e-258	89.36	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
A0A6J1IJ76	2.9e-256	88.39	tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 S...	[more]

BLAST of MS021816 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20370.1	5.0e-152	58.28	Pseudouridine synthase family protein	[more]
AT1G76120.1	3.7e-147	59.62	Pseudouridine synthase family protein	[more]
AT1G76120.2	4.7e-110	57.11	Pseudouridine synthase family protein	[more]
AT2G30320.1	1.2e-25	26.76	Pseudouridine synthase family protein	[more]
AT1G20290.1	1.5e-10	52.46	SWI-SNF-related chromatin binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 45..165 e-value: 1.7E-28 score: 101.1
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 327..432 e-value: 2.5E-15 score: 56.8
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 294..496 e-value: 1.4E-55 score: 190.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 250..306
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..41
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 230..314
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR11142:SF24	TRNA PSEUDOURIDINE SYNTHASE	coord: 31..494
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 31..494
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 59..432 e-value: 4.02216E-106 score: 315.322
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..443

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021816.1	MS021816.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:0031119	tRNA pseudouridine synthesis
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding