Homology
BLAST of MS021814 vs. NCBI nr
Match:
XP_022133468.1 (prolyl endopeptidase-like [Momordica charantia])
HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 782/786 (99.49%), Postives = 784/786 (99.75%), Query Frame = 0
Query: 1 PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL 60
PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL
Sbjct: 14 PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL 73
Query: 61 SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC 120
SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC
Sbjct: 74 SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC 133
Query: 121 ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN 180
ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN
Sbjct: 134 ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN 193
Query: 181 ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS 240
ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS
Sbjct: 194 ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS 253
Query: 241 WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 300
WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL
Sbjct: 254 WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 313
Query: 301 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 360
FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ
Sbjct: 314 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 373
Query: 361 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIV 420
YHAIANDDTLFTFITNKNAPKYKLVRVDLKDP VWTELLPESEKDVLESATAVNGDQMIV
Sbjct: 374 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPMVWTELLPESEKDVLESATAVNGDQMIV 433
Query: 421 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 480
SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN
Sbjct: 434 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 493
Query: 481 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 540
LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY
Sbjct: 494 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 553
Query: 541 GYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 600
GYGGFNINITPSFS+SRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI
Sbjct: 554 GYGGFNINITPSFSLSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 613
Query: 601 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 660
SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI
Sbjct: 614 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 673
Query: 661 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPL 720
GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPD+FVQYPATMLLTADHDDRVVPL
Sbjct: 674 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDQFVQYPATMLLTADHDDRVVPL 733
Query: 721 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTL 780
HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAK L
Sbjct: 734 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKML 793
Query: 781 EATWID 787
EATWID
Sbjct: 794 EATWID 799
BLAST of MS021814 vs. NCBI nr
Match:
XP_038897426.1 (prolyl endopeptidase [Benincasa hispida])
HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 705/783 (90.04%), Postives = 741/783 (94.64%), Query Frame = 0
Query: 8 YIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPS---SSSFKFLSSSRPNMGSLSALL 67
YIPL S SP FAPRL+ P RL + + LSAFPS SSSF LS R MGSLSA+L
Sbjct: 10 YIPL--SLSPFFAPRLQPLPLRLRLLQVLSLSAFPSLHHSSSFFNLSLRR--MGSLSAML 69
Query: 68 HPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESRE 127
PL YPTARRD+S+V+DYHGVQIADPYRWLEDPDA+EVKEFVQKQVKLTESVLQKC++RE
Sbjct: 70 DPLLYPTARRDDSVVEDYHGVQIADPYRWLEDPDADEVKEFVQKQVKLTESVLQKCDTRE 129
Query: 128 KLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSE 187
KLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEP VLLDPNALSE
Sbjct: 130 KLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPEVLLDPNALSE 189
Query: 188 DGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVD 247
DGTVSLS LSVSKDAK +AYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWVKFS ISWTVD
Sbjct: 190 DGTVSLSNLSVSKDAKCLAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWVKFSSISWTVD 249
Query: 248 GKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSA 307
GKGFFYSRYPAPKE G +DAGTET+ANLYHE+YYHFLGSDQSDDVLCWRD DHPKYLFSA
Sbjct: 250 GKGFFYSRYPAPKEVGTLDAGTETNANLYHELYYHFLGSDQSDDVLCWRDQDHPKYLFSA 309
Query: 308 TVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHA 367
+VTDDGKYVL+E +EGCDPVNKFYYC++SALPNGLEGF+GK+DLLPFTKLID+FDAQYHA
Sbjct: 310 SVTDDGKYVLMEIEEGCDPVNKFYYCNISALPNGLEGFKGKNDLLPFTKLIDNFDAQYHA 369
Query: 368 IANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIVSYL 427
IANDDTLFTFITNKNAPKYKLVRVDL DPTVWTELLPESEKDVLESA AVNGDQMIVSYL
Sbjct: 370 IANDDTLFTFITNKNAPKYKLVRVDLNDPTVWTELLPESEKDVLESACAVNGDQMIVSYL 429
Query: 428 SDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLES 487
SDVKYVLQIRDLKSGSLLH+LPIDIGSV GISARREDSLIFIGFTSFLTPGIIYQCNLE+
Sbjct: 430 SDVKYVLQIRDLKSGSLLHQLPIDIGSVYGISARREDSLIFIGFTSFLTPGIIYQCNLET 489
Query: 488 GIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYG 547
G PDMK+FREIVVPGFERSEFNV+QVFV SKDGTNIPMFIVARKNIVLDGSHPCLLYGYG
Sbjct: 490 GTPDMKIFREIVVPGFERSEFNVDQVFVRSKDGTNIPMFIVARKNIVLDGSHPCLLYGYG 549
Query: 548 GFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSA 607
GFNIN+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSA
Sbjct: 550 GFNINLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSA 609
Query: 608 EYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHA 667
EYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDMLRFHKFTIGHA
Sbjct: 610 EYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDMLRFHKFTIGHA 669
Query: 668 WTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSL 727
WTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADHDDRVVPLHSL
Sbjct: 670 WTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRLLQYPSTMLLTADHDDRVVPLHSL 729
Query: 728 KLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEAT 787
KLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRYAFMAK L A
Sbjct: 730 KLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRYAFMAKMLAAA 788
BLAST of MS021814 vs. NCBI nr
Match:
KAG7037934.1 (Prolyl endopeptidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1428.3 bits (3696), Expect = 0.0e+00
Identity = 693/793 (87.39%), Postives = 740/793 (93.32%), Query Frame = 0
Query: 6 YTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIP--LSAFP-------SSSSFK--FLSSSR 65
++++ SFSPLFAPRL+L R LQVSS+P LSA P SSSS + SSSR
Sbjct: 5 WSHLVAPLSFSPLFAPRLQL--LRRLQVSSLPSFLSALPPSYSPPSSSSSLRPSSFSSSR 64
Query: 66 PNMGSLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTE 125
MGSLSAL PL YPT+RRD+S+V+DYHGV+IADPYRWLEDPDA+EVKEFVQKQV LTE
Sbjct: 65 RRMGSLSALHEPLRYPTSRRDDSVVEDYHGVRIADPYRWLEDPDADEVKEFVQKQVTLTE 124
Query: 126 SVLQKCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPA 185
SVLQKC++REKLRAKIT+LFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEP
Sbjct: 125 SVLQKCDTREKLRAKITQLFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPE 184
Query: 186 VLLDPNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWV 245
VLLDPNALSEDGTVSLS+LS+SKDAKYMAYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWV
Sbjct: 185 VLLDPNALSEDGTVSLSSLSISKDAKYMAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWV 244
Query: 246 KFSGISWTVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRD 305
KFS ISWTVDGKGFFYSRYPAP+E G +DAGTET+ANLYHE+YYHFLGSDQSDDVLCWRD
Sbjct: 245 KFSSISWTVDGKGFFYSRYPAPREVGTLDAGTETNANLYHELYYHFLGSDQSDDVLCWRD 304
Query: 306 PDHPKYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKL 365
DHPKYLFS VT+DGKYVL+E EGCDPVNKFYYC++S LPNGLEGFR K+DLLPFTKL
Sbjct: 305 QDHPKYLFSGRVTEDGKYVLMEISEGCDPVNKFYYCNISELPNGLEGFREKNDLLPFTKL 364
Query: 366 IDDFDAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAV 425
IDDFDAQYHAIANDD+LFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESA AV
Sbjct: 365 IDDFDAQYHAIANDDSLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESACAV 424
Query: 426 NGDQMIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTP 485
NGDQ+IVSYLSDVKYVLQIR+LKSGSLLH+LPIDIGSV GISARREDSL+FIGFTSFLTP
Sbjct: 425 NGDQVIVSYLSDVKYVLQIRELKSGSLLHQLPIDIGSVYGISARREDSLVFIGFTSFLTP 484
Query: 486 GIIYQCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDG 545
GIIYQCNLE+G PDMK+FREI VPGFERSEF V+QVFV SKDG IPMFIVARKNIVLDG
Sbjct: 485 GIIYQCNLETGTPDMKIFREIAVPGFERSEFEVDQVFVSSKDGAKIPMFIVARKNIVLDG 544
Query: 546 SHPCLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQ 605
SHPCLLYGYGGFN+N+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSL+KKQ
Sbjct: 545 SHPCLLYGYGGFNVNLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLSKKQ 604
Query: 606 NCFDDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDML 665
NCFDDFISSAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDML
Sbjct: 605 NCFDDFISSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDML 664
Query: 666 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADH 725
RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADH
Sbjct: 665 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRPLQYPSTMLLTADH 724
Query: 726 DDRVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRY 785
DDRVVPLHSLKLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRY
Sbjct: 725 DDRVVPLHSLKLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRY 784
Query: 786 AFMAKTLEATWID 787
AFMAK L ATWID
Sbjct: 785 AFMAKMLSATWID 795
BLAST of MS021814 vs. NCBI nr
Match:
KAG6608618.1 (Prolyl endopeptidase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 693/793 (87.39%), Postives = 739/793 (93.19%), Query Frame = 0
Query: 6 YTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIP--LSAFP-------SSSSFK--FLSSSR 65
++++ SFSPLFAPRL+L R LQVSS+P LSA P SSSS + SSSR
Sbjct: 5 WSHLVAPLSFSPLFAPRLQL--LRRLQVSSLPSFLSALPPSYSPPSSSSSLRPSSFSSSR 64
Query: 66 PNMGSLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTE 125
MGSLSAL PL YPT+RRD+S+V+DYHGV IADPYRWLEDPDA+EVKEFVQKQV LTE
Sbjct: 65 RRMGSLSALHEPLRYPTSRRDDSVVEDYHGVGIADPYRWLEDPDADEVKEFVQKQVTLTE 124
Query: 126 SVLQKCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPA 185
SVLQKC++REKLRAKIT+LFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEP
Sbjct: 125 SVLQKCDTREKLRAKITQLFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPE 184
Query: 186 VLLDPNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWV 245
VLLDPNALSEDGTVSLS+LS+SKDAKYMAYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWV
Sbjct: 185 VLLDPNALSEDGTVSLSSLSISKDAKYMAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWV 244
Query: 246 KFSGISWTVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRD 305
KFS ISWTVDGKGFFYSRYPAP+E G +DAGTET+ANLYHE+YYHFLGSDQSDDVLCWRD
Sbjct: 245 KFSSISWTVDGKGFFYSRYPAPREVGTLDAGTETNANLYHELYYHFLGSDQSDDVLCWRD 304
Query: 306 PDHPKYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKL 365
DHPKYLFS VT+DGKYVL+E EGCDPVNKFYYC++S LPNGLEGFR K+DLLPFTKL
Sbjct: 305 QDHPKYLFSGRVTEDGKYVLMEISEGCDPVNKFYYCNISELPNGLEGFREKNDLLPFTKL 364
Query: 366 IDDFDAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAV 425
IDDFDAQYHAIANDD+LFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESA AV
Sbjct: 365 IDDFDAQYHAIANDDSLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESACAV 424
Query: 426 NGDQMIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTP 485
NGDQ+IVSYLSDVKYVLQIR+LKSGSLLH+LPIDIGSV GISARREDSL+FIGFTSFLTP
Sbjct: 425 NGDQVIVSYLSDVKYVLQIRELKSGSLLHQLPIDIGSVYGISARREDSLVFIGFTSFLTP 484
Query: 486 GIIYQCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDG 545
GIIYQCNLE+G PDMK+FREI VPGFERSEF V+QVFV SKDG IPMFIVARKNIVLDG
Sbjct: 485 GIIYQCNLETGTPDMKIFREIAVPGFERSEFEVDQVFVSSKDGAKIPMFIVARKNIVLDG 544
Query: 546 SHPCLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQ 605
SHPCLLYGYGGFN+N+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSL+KKQ
Sbjct: 545 SHPCLLYGYGGFNVNLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLSKKQ 604
Query: 606 NCFDDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDML 665
NCFDDFISSAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDML
Sbjct: 605 NCFDDFISSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDML 664
Query: 666 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADH 725
RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADH
Sbjct: 665 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRPLQYPSTMLLTADH 724
Query: 726 DDRVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRY 785
DDRVVPLHSLKLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRY
Sbjct: 725 DDRVVPLHSLKLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRY 784
Query: 786 AFMAKTLEATWID 787
AFMAK L ATWID
Sbjct: 785 AFMAKMLSATWID 795
BLAST of MS021814 vs. NCBI nr
Match:
XP_022981810.1 (prolyl endopeptidase-like [Cucurbita maxima])
HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 691/786 (87.91%), Postives = 737/786 (93.77%), Query Frame = 0
Query: 14 SFSPLFAPRLRLQPFRLLQVSSIP--LSAFP--------SSSSFK--FLSSSRPNMGSLS 73
SFSPLFAPRL+L RLLQVSS+P LSA P SSSS + SSSR MGSLS
Sbjct: 13 SFSPLFAPRLQL--LRLLQVSSVPSFLSALPPSYSPPSSSSSSLRPSSFSSSRRRMGSLS 72
Query: 74 ALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCE 133
AL PL YPT+RRD+S+V+DYHGV+IADPYRWLEDPDA+EVKEFVQKQV LTESVLQKC+
Sbjct: 73 ALHEPLRYPTSRRDDSVVEDYHGVRIADPYRWLEDPDADEVKEFVQKQVTLTESVLQKCD 132
Query: 134 SREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNA 193
+R+KLRAKIT+LFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEP VLLDPNA
Sbjct: 133 TRQKLRAKITQLFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPEVLLDPNA 192
Query: 194 LSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISW 253
LS+DGTVSLS+LS+S+DAKYMAYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWVKFS ISW
Sbjct: 193 LSKDGTVSLSSLSISQDAKYMAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWVKFSSISW 252
Query: 254 TVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 313
TVDGKGFFYSRYPAP+E G +DAGTET+ANLYHE+YYHFLGSDQSDDVLCWRD DHPKYL
Sbjct: 253 TVDGKGFFYSRYPAPREVGTLDAGTETNANLYHELYYHFLGSDQSDDVLCWRDQDHPKYL 312
Query: 314 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 373
FS VT+DGKYVL+E EGCDPVNKFYYC++S LPNGLEGFR K+DLLPFTKLIDDFDAQ
Sbjct: 313 FSGRVTEDGKYVLMEISEGCDPVNKFYYCNISELPNGLEGFREKNDLLPFTKLIDDFDAQ 372
Query: 374 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIV 433
YHAIANDD+LFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESA AVNGDQ+IV
Sbjct: 373 YHAIANDDSLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESACAVNGDQVIV 432
Query: 434 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 493
SYLSDVKYVLQIRDLKSGSLLH+LP+DIGSV GISARREDSL+FIGFTSFLTPGIIYQCN
Sbjct: 433 SYLSDVKYVLQIRDLKSGSLLHQLPVDIGSVYGISARREDSLVFIGFTSFLTPGIIYQCN 492
Query: 494 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 553
LE+G PDMK+FREI VPGFERSEF V+QVFV SKDGT IPMFIVARKNIVLDGSHPCLLY
Sbjct: 493 LETGTPDMKIFREIAVPGFERSEFEVDQVFVSSKDGTKIPMFIVARKNIVLDGSHPCLLY 552
Query: 554 GYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 613
GYGGFN+N+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSL+KKQNCFDDFI
Sbjct: 553 GYGGFNVNLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLSKKQNCFDDFI 612
Query: 614 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 673
SAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDMLRFHKFTI
Sbjct: 613 FSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDMLRFHKFTI 672
Query: 674 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPL 733
GHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADHDDRVVPL
Sbjct: 673 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRPLQYPSTMLLTADHDDRVVPL 732
Query: 734 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTL 787
HSLKLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRYAFMAK L
Sbjct: 733 HSLKLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRYAFMAKML 792
BLAST of MS021814 vs. ExPASy Swiss-Prot
Match:
P48147 (Prolyl endopeptidase OS=Homo sapiens OX=9606 GN=PREP PE=1 SV=2)
HSP 1 Score: 866.7 bits (2238), Expect = 2.0e-250
Identity = 402/721 (55.76%), Postives = 529/721 (73.37%), Query Frame = 0
Query: 67 LHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKL 126
L YP RDE+ V DYHG +I DPY WLEDPD+E+ K FV+ Q K+T L++C R
Sbjct: 4 LQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLY 63
Query: 127 RAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDG 186
+ ++TEL+D+P+Y FK+G +YFYF+NTGLQ Q VLYVQDSL+GE V LDPN LS+DG
Sbjct: 64 KERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDG 123
Query: 187 TVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGK 246
TV+L + S+D +Y AYGLS+SGSDWVTIK M+VD K PD L VKFS ++WT DGK
Sbjct: 124 TVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGK 183
Query: 247 GFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVT 306
G FY+ Y P++ G GTET NL+ ++YYH LG+DQS+D+LC PD PK++ A ++
Sbjct: 184 GMFYNSY--PQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELS 243
Query: 307 DDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIAN 366
DDG+YVLL EGCDPVN+ +YCD+ +G+ G +L + KLID+F+ +Y + N
Sbjct: 244 DDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAG------ILKWVKLIDNFEGEYDYVTN 303
Query: 367 DDTLFTFITNKNAPKYKLVRVDLKDP--TVWTELLPESEKDVLESATAVNGDQMIVSYLS 426
+ T+FTF TN+ +P Y+++ +D +DP + W L+PE EKDVLE V + +++ YL
Sbjct: 304 EGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLH 363
Query: 427 DVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESG 486
DVK +LQ+ DL +G+LL P+D+GS+ G S +++D+ IF FTSFL+PGIIY C+L
Sbjct: 364 DVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKE 423
Query: 487 IPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGG 546
+ +VFRE+ V G + S++ Q+F SKDGT IPMFIV +K I LDGSHP LYGYGG
Sbjct: 424 ELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGG 483
Query: 547 FNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAE 606
FNI+ITP++SVSR++ RH+G I +ANIRGGGEYGE WHK G LA KQNCFDDF +AE
Sbjct: 484 FNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAE 543
Query: 607 YLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAW 666
YLI GYT P +L I GGSNGGLLV AC NQRPDL+GC +A VGVMDML+FHK+TIGHAW
Sbjct: 544 YLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAW 603
Query: 667 TSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLK 726
T+DYGCSD+++ F+WL+KYSPLHNV+ P +QYP+ +LLTADHDDRVVPLHSLK
Sbjct: 604 TTDYGCSDSKQHFEWLVKYSPLHNVKLPEADD----IQYPSMLLLTADHDDRVVPLHSLK 663
Query: 727 LLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATW 786
+AT+QY++ S + Q+NP++ ++ KAGHGAG+PT K+I+E SD +AF+A+ L W
Sbjct: 664 FIATLQYIVGRSRK---QSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDW 709
BLAST of MS021814 vs. ExPASy Swiss-Prot
Match:
Q9QUR6 (Prolyl endopeptidase OS=Mus musculus OX=10090 GN=Prep PE=1 SV=1)
HSP 1 Score: 865.9 bits (2236), Expect = 3.5e-250
Identity = 403/719 (56.05%), Postives = 526/719 (73.16%), Query Frame = 0
Query: 69 YPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRA 128
YP RDE+ V +YHG +I DPY WLEDPD+E+ K FV+ Q K+T L++C R +
Sbjct: 6 YPDVYRDETSVQEYHGHKICDPYSWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKE 65
Query: 129 KITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTV 188
++TEL+D+P+Y FK+G +YFYF+NTGLQ Q VLYVQDSL+GE V LDPN LS+DGTV
Sbjct: 66 RMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNTLSDDGTV 125
Query: 189 SLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGF 248
+L + S+D +Y AYGLS+SGSDWVTIK M+VD K PD L VKF+ ++WT DGKG
Sbjct: 126 ALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFTCMAWTHDGKGM 185
Query: 249 FYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDD 308
FY+ Y P++ G GTET NL+ ++ YH LG+DQS+D+LC PD PK++ A ++DD
Sbjct: 186 FYNSY--PQQDGKSDGTETSTNLHQKLCYHVLGTDQSEDILCAEFPDEPKWMGGAELSDD 245
Query: 309 GKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDD 368
G+YVLL EGCDPVN+ +YCD+ PNG+ G +L + KLID+F+ +Y + N+
Sbjct: 246 GRYVLLSIWEGCDPVNRLWYCDLQQEPNGITG------ILKWVKLIDNFEGEYDYVTNEG 305
Query: 369 TLFTFITNKNAPKYKLVRVDLKDP--TVWTELLPESEKDVLESATAVNGDQMIVSYLSDV 428
T+FTF TN+N+P Y+L+ +D DP + W L+PE EKDVLE V + +++ YL DV
Sbjct: 306 TVFTFKTNRNSPNYRLINIDFTDPDESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLHDV 365
Query: 429 KYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIP 488
K +LQ+ DL +G+LL P+D+GSV G S R++DS IF FTSFL+PG+IY C+L
Sbjct: 366 KNILQLHDLTTGALLKTFPLDVGSVVGYSGRKKDSEIFYQFTSFLSPGVIYHCDLTKEEL 425
Query: 489 DMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFN 548
+ VFRE+ V G + +++ Q+F SKDGT IPMFIV +K I LDGSHP LYGYGGFN
Sbjct: 426 EPMVFREVTVKGIDAADYQTIQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFN 485
Query: 549 INITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYL 608
I+ITP++SVSR++ RH+G + +ANIRGGGEYGE WHK G LA KQNCFDDF +AEYL
Sbjct: 486 ISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYL 545
Query: 609 ISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTS 668
I GYT P +L I GGSNGGLLV AC NQRPDL+GC +A VGVMDML+FHKFTIGHAWT+
Sbjct: 546 IKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKFTIGHAWTT 605
Query: 669 DYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLL 728
DYGCSD ++ F+WL+KYSPLHNV+ P +QYP+ +LLTADHDDRVVPLHSLK +
Sbjct: 606 DYGCSDTKQHFEWLLKYSPLHNVKLPEADD----IQYPSMLLLTADHDDRVVPLHSLKFI 665
Query: 729 ATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWI 786
AT+QY++ S + Q+NP++ ++ KAGHGAG+PT K+I+E SD +AF+A+ L WI
Sbjct: 666 ATLQYIVGRSRK---QSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNIEWI 709
BLAST of MS021814 vs. ExPASy Swiss-Prot
Match:
P23687 (Prolyl endopeptidase OS=Sus scrofa OX=9823 GN=PREP PE=1 SV=1)
HSP 1 Score: 861.7 bits (2225), Expect = 6.5e-249
Identity = 400/719 (55.63%), Postives = 525/719 (73.02%), Query Frame = 0
Query: 69 YPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRA 128
YP RDE+ + DYHG ++ DPY WLEDPD+E+ K FV+ Q K+T L++C R +
Sbjct: 6 YPDVYRDETAIQDYHGHKVCDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKE 65
Query: 129 KITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTV 188
++TEL+D+P+Y FK+G +YFYF+NTGLQ Q VLYVQDSL+GE V LDPN LS+DGTV
Sbjct: 66 RMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTV 125
Query: 189 SLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGF 248
+L + S+D +Y AYGLS+SGSDWVTIK M+VD K PD L VKFS ++WT DGKG
Sbjct: 126 ALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGM 185
Query: 249 FYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDD 308
FY+ Y P++ G GTET NL+ ++YYH LG+DQS+D+LC PD PK++ A ++DD
Sbjct: 186 FYNAY--PQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDD 245
Query: 309 GKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDD 368
G+YVLL EGCDPVN+ +YCD+ NG+ G +L + KLID+F+ +Y + N+
Sbjct: 246 GRYVLLSIREGCDPVNRLWYCDLQQESNGITG------ILKWVKLIDNFEGEYDYVTNEG 305
Query: 369 TLFTFITNKNAPKYKLVRVDLKDP--TVWTELLPESEKDVLESATAVNGDQMIVSYLSDV 428
T+FTF TN+++P Y+L+ +D DP + W L+PE EKDVLE V + +++ YL DV
Sbjct: 306 TVFTFKTNRHSPNYRLINIDFTDPEESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLHDV 365
Query: 429 KYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIP 488
K LQ+ DL +G+LL P+++GSV G S +++D+ IF FTSFL+PGIIY C+L
Sbjct: 366 KNTLQLHDLATGALLKIFPLEVGSVVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEEL 425
Query: 489 DMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFN 548
+ +VFRE+ V G + S++ Q+F SKDGT IPMFIV +K I LDGSHP LYGYGGFN
Sbjct: 426 EPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFN 485
Query: 549 INITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYL 608
I+ITP++SVSR++ RH+G + +ANIRGGGEYGE WHK G LA KQNCFDDF +AEYL
Sbjct: 486 ISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYL 545
Query: 609 ISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTS 668
I GYT P +L I GGSNGGLLV C NQRPDL+GC +A VGVMDML+FHK+TIGHAWT+
Sbjct: 546 IKEGYTSPKRLTINGGSNGGLLVATCANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTT 605
Query: 669 DYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLL 728
DYGCSD+++ F+WLIKYSPLHNV+ P +QYP+ +LLTADHDDRVVPLHSLK +
Sbjct: 606 DYGCSDSKQHFEWLIKYSPLHNVKLPEADD----IQYPSMLLLTADHDDRVVPLHSLKFI 665
Query: 729 ATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWI 786
AT+QY++ S + Q NP++ ++ KAGHGAG+PT K+I+E SD +AF+A+ L WI
Sbjct: 666 ATLQYIVGRSRK---QNNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNIDWI 709
BLAST of MS021814 vs. ExPASy Swiss-Prot
Match:
O70196 (Prolyl endopeptidase OS=Rattus norvegicus OX=10116 GN=Prep PE=1 SV=1)
HSP 1 Score: 860.9 bits (2223), Expect = 1.1e-248
Identity = 404/719 (56.19%), Postives = 525/719 (73.02%), Query Frame = 0
Query: 69 YPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRA 128
YP RDE+ V DYHG +I DPY WLEDPD+E+ K FV+ Q K+T L++C R +
Sbjct: 6 YPDVYRDETSVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKE 65
Query: 129 KITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTV 188
++TEL+D+P+Y FK+G +YFYF+NTGLQ Q VLYVQDSL+GE V LDPN LS+DGTV
Sbjct: 66 RMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNTLSDDGTV 125
Query: 189 SLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGF 248
+L + S+D +Y AYGLS+SGSDWVTIK M+VD K PD L VKF+ ++WT DGKG
Sbjct: 126 ALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFTCMAWTHDGKGM 185
Query: 249 FYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDD 308
FY+ Y P++ G GTET NL+ ++ YH LG+DQS+DVLC PD PK++ A ++DD
Sbjct: 186 FYNSY--PQQDGKSDGTETSTNLHQKLCYHVLGTDQSEDVLCAEFPDEPKWMGGAELSDD 245
Query: 309 GKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDD 368
G+YVLL EGCDPVN+ +YCD+ NG+ G +L + KLID+F+ +Y I N+
Sbjct: 246 GRYVLLSIWEGCDPVNRLWYCDLQQGSNGING------ILKWVKLIDNFEGEYDYITNEG 305
Query: 369 TLFTFITNKNAPKYKLVRVDLKDP--TVWTELLPESEKDVLESATAVNGDQMIVSYLSDV 428
T+FTF TN+N+P Y+L+ +D DP + W L+PE EKDVLE V + +++ YL +V
Sbjct: 306 TVFTFKTNRNSPNYRLINIDFTDPDESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLRNV 365
Query: 429 KYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIP 488
K +LQ+ DL +G+LL P+D+GSV G S R++DS IF FTSFL+PG+IY C+L
Sbjct: 366 KNILQLHDLTTGALLKTFPLDVGSVVGYSGRKKDSEIFYQFTSFLSPGVIYHCDLTREEL 425
Query: 489 DMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFN 548
+ +VFRE+ V G + S++ QVF SKDGT IPMFIV +K I LDGSHP LYGYGGFN
Sbjct: 426 EPRVFREVTVKGIDASDYQTIQVFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFN 485
Query: 549 INITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYL 608
I+ITP++SVSR++ RH+G + +ANIRGGGEYGE WHK G LA KQNCFDDF +AEYL
Sbjct: 486 ISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYL 545
Query: 609 ISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTS 668
I GYT +L I GGSNGGLLV AC NQRPDL+GC +A VGVMDML+FHKFTIGHAWT+
Sbjct: 546 IKEGYTTSKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKFTIGHAWTT 605
Query: 669 DYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLL 728
DYGCSD+++ F+WL+KYSPLHNV+ P +QYP+ +LLTADHDDRVVPLHSLK +
Sbjct: 606 DYGCSDSKQHFEWLLKYSPLHNVKLPEADD----IQYPSMLLLTADHDDRVVPLHSLKFI 665
Query: 729 ATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWI 786
AT+QY++ S + Q+NP++ ++ KAGHG G+PT K+I+E SD +AF+A+ L WI
Sbjct: 666 ATLQYIVGRSRK---QSNPLLIHVDTKAGHGPGKPTAKVIEEVSDMFAFIARCLNIEWI 709
BLAST of MS021814 vs. ExPASy Swiss-Prot
Match:
Q9XTA2 (Prolyl endopeptidase OS=Bos taurus OX=9913 GN=PREP PE=2 SV=1)
HSP 1 Score: 853.2 bits (2203), Expect = 2.3e-246
Identity = 398/719 (55.35%), Postives = 523/719 (72.74%), Query Frame = 0
Query: 69 YPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRA 128
YP RDE+ V DYHG +I DPY WLEDPD+E+ K FV+ Q K+T L++C R +
Sbjct: 6 YPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKE 65
Query: 129 KITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTV 188
++TEL+D+P+Y FK+G +YFYF+NTGLQ Q VLYVQDSL+GE V LDPN LS+DGTV
Sbjct: 66 RMTELYDYPKYSCNFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVCLDPNTLSDDGTV 125
Query: 189 SLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGF 248
+L + S+D +Y+AYGLS+SGSDWVTIK M+VD K D L VKFS ++WT DGKG
Sbjct: 126 ALRGYAFSEDGEYVAYGLSASGSDWVTIKFMKVDGAKELADVLERVKFSCMAWTHDGKGM 185
Query: 249 FYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDD 308
FY+ Y P++ G GTET NL+ ++ YH LG+DQS+D+LC PD PK++ A ++DD
Sbjct: 186 FYNAY--PQQDGKSDGTETSTNLHQKLCYHVLGTDQSEDILCAEFPDEPKWMGGAELSDD 245
Query: 309 GKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDD 368
G+YVLL EGCDPVN+ +YCD+ PNG+ G +L + KLID+F+ +Y + N+
Sbjct: 246 GRYVLLSIREGCDPVNRLWYCDLHQEPNGITG------ILKWVKLIDNFEGEYDYVTNEG 305
Query: 369 TLFTFITNKNAPKYKLVRVDLKDP--TVWTELLPESEKDVLESATAVNGDQMIVSYLSDV 428
T+FTF TN+++P Y+L+ +D DP + W L+PE EKDVLE V + +++ YL DV
Sbjct: 306 TVFTFKTNRHSPNYRLINIDFTDPEESRWKVLVPEHEKDVLEWVACVRSNFLVLCYLHDV 365
Query: 429 KYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIP 488
K LQ+ D+ +G+LL P+++GSV G S +++D+ IF FTSFL+PGIIY C+L
Sbjct: 366 KNTLQLHDMATGALLKTFPLEVGSVVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEEL 425
Query: 489 DMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFN 548
+ +VFRE+ V G + S++ Q+F SKDGT IPMFIV +K I LDGSHP LYGYGGFN
Sbjct: 426 EPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFN 485
Query: 549 INITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYL 608
I+ITP++SV R++ RH+G + +ANIRGGGEYGE WHK G LA KQNCFDDF +AEYL
Sbjct: 486 ISITPNYSVCRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYL 545
Query: 609 ISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTS 668
I GYT P +L I GGSNGGLLV C NQRPDL+GC +A VGVMDML+FHK+TIGHAWT+
Sbjct: 546 IKEGYTSPKRLTINGGSNGGLLVATCANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTT 605
Query: 669 DYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLL 728
DYGCSDN++ F+WLIKYSPLHNV+ P +QYP+ +LLTADHDDRVVPLHS K +
Sbjct: 606 DYGCSDNKQHFEWLIKYSPLHNVKLPEADD----IQYPSMLLLTADHDDRVVPLHSPKFI 665
Query: 729 ATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWI 786
AT+Q+++ S + Q NP++ ++ KAGHGAG+PT K+I+E SD +AF+A+ L WI
Sbjct: 666 ATLQHLVGRSRK---QNNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNIDWI 709
BLAST of MS021814 vs. ExPASy TrEMBL
Match:
A0A6J1BZ76 (Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111006037 PE=3 SV=1)
HSP 1 Score: 1610.5 bits (4169), Expect = 0.0e+00
Identity = 782/786 (99.49%), Postives = 784/786 (99.75%), Query Frame = 0
Query: 1 PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL 60
PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL
Sbjct: 14 PLAFRYTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMGSL 73
Query: 61 SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC 120
SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC
Sbjct: 74 SALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKC 133
Query: 121 ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN 180
ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN
Sbjct: 134 ESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPN 193
Query: 181 ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS 240
ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS
Sbjct: 194 ALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGIS 253
Query: 241 WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 300
WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL
Sbjct: 254 WTVDGKGFFYSRYPAPKEAGVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 313
Query: 301 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 360
FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ
Sbjct: 314 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 373
Query: 361 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIV 420
YHAIANDDTLFTFITNKNAPKYKLVRVDLKDP VWTELLPESEKDVLESATAVNGDQMIV
Sbjct: 374 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPMVWTELLPESEKDVLESATAVNGDQMIV 433
Query: 421 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 480
SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN
Sbjct: 434 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 493
Query: 481 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 540
LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY
Sbjct: 494 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 553
Query: 541 GYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 600
GYGGFNINITPSFS+SRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI
Sbjct: 554 GYGGFNINITPSFSLSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 613
Query: 601 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 660
SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI
Sbjct: 614 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 673
Query: 661 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPL 720
GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPD+FVQYPATMLLTADHDDRVVPL
Sbjct: 674 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDQFVQYPATMLLTADHDDRVVPL 733
Query: 721 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTL 780
HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAK L
Sbjct: 734 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKML 793
Query: 781 EATWID 787
EATWID
Sbjct: 794 EATWID 799
BLAST of MS021814 vs. ExPASy TrEMBL
Match:
A0A6J1J0P1 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111480845 PE=3 SV=1)
HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 691/786 (87.91%), Postives = 737/786 (93.77%), Query Frame = 0
Query: 14 SFSPLFAPRLRLQPFRLLQVSSIP--LSAFP--------SSSSFK--FLSSSRPNMGSLS 73
SFSPLFAPRL+L RLLQVSS+P LSA P SSSS + SSSR MGSLS
Sbjct: 13 SFSPLFAPRLQL--LRLLQVSSVPSFLSALPPSYSPPSSSSSSLRPSSFSSSRRRMGSLS 72
Query: 74 ALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCE 133
AL PL YPT+RRD+S+V+DYHGV+IADPYRWLEDPDA+EVKEFVQKQV LTESVLQKC+
Sbjct: 73 ALHEPLRYPTSRRDDSVVEDYHGVRIADPYRWLEDPDADEVKEFVQKQVTLTESVLQKCD 132
Query: 134 SREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNA 193
+R+KLRAKIT+LFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEP VLLDPNA
Sbjct: 133 TRQKLRAKITQLFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPEVLLDPNA 192
Query: 194 LSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISW 253
LS+DGTVSLS+LS+S+DAKYMAYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWVKFS ISW
Sbjct: 193 LSKDGTVSLSSLSISQDAKYMAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWVKFSSISW 252
Query: 254 TVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYL 313
TVDGKGFFYSRYPAP+E G +DAGTET+ANLYHE+YYHFLGSDQSDDVLCWRD DHPKYL
Sbjct: 253 TVDGKGFFYSRYPAPREVGTLDAGTETNANLYHELYYHFLGSDQSDDVLCWRDQDHPKYL 312
Query: 314 FSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQ 373
FS VT+DGKYVL+E EGCDPVNKFYYC++S LPNGLEGFR K+DLLPFTKLIDDFDAQ
Sbjct: 313 FSGRVTEDGKYVLMEISEGCDPVNKFYYCNISELPNGLEGFREKNDLLPFTKLIDDFDAQ 372
Query: 374 YHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIV 433
YHAIANDD+LFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESA AVNGDQ+IV
Sbjct: 373 YHAIANDDSLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESACAVNGDQVIV 432
Query: 434 SYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN 493
SYLSDVKYVLQIRDLKSGSLLH+LP+DIGSV GISARREDSL+FIGFTSFLTPGIIYQCN
Sbjct: 433 SYLSDVKYVLQIRDLKSGSLLHQLPVDIGSVYGISARREDSLVFIGFTSFLTPGIIYQCN 492
Query: 494 LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLY 553
LE+G PDMK+FREI VPGFERSEF V+QVFV SKDGT IPMFIVARKNIVLDGSHPCLLY
Sbjct: 493 LETGTPDMKIFREIAVPGFERSEFEVDQVFVSSKDGTKIPMFIVARKNIVLDGSHPCLLY 552
Query: 554 GYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFI 613
GYGGFN+N+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSL+KKQNCFDDFI
Sbjct: 553 GYGGFNVNLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLSKKQNCFDDFI 612
Query: 614 SSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTI 673
SAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDMLRFHKFTI
Sbjct: 613 FSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDMLRFHKFTI 672
Query: 674 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPL 733
GHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADHDDRVVPL
Sbjct: 673 GHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRPLQYPSTMLLTADHDDRVVPL 732
Query: 734 HSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTL 787
HSLKLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRYAFMAK L
Sbjct: 733 HSLKLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRYAFMAKML 792
BLAST of MS021814 vs. ExPASy TrEMBL
Match:
A0A1S3BV12 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103493792 PE=3 SV=1)
HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 691/789 (87.58%), Postives = 731/789 (92.65%), Query Frame = 0
Query: 2 LAFRYT---YIPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMG 61
++FR+ IPL SFSPLF PRL P L S P SSS F + SR MG
Sbjct: 2 VSFRFALPYIIPL--SFSPLFPPRL---PLSSLSSLSSPFPFLRPSSS--FFNHSRRRMG 61
Query: 62 SLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQ 121
SLSAL+ P YPTARRD+S+VDDYHG QI DPYRWLEDPDA+EVKEFV+KQVKLTESVLQ
Sbjct: 62 SLSALIDPFLYPTARRDDSVVDDYHGFQIPDPYRWLEDPDADEVKEFVEKQVKLTESVLQ 121
Query: 122 KCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLD 181
KC++REKLRAKITELFDHPRYEPPFKRGNKYFY+HNTGLQAQSVLYVQ+SLDGEP VLLD
Sbjct: 122 KCDTREKLRAKITELFDHPRYEPPFKRGNKYFYYHNTGLQAQSVLYVQNSLDGEPEVLLD 181
Query: 182 PNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSG 241
PNALSEDGTVSLS LSVSKDAKY+AYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFS
Sbjct: 182 PNALSEDGTVSLSNLSVSKDAKYLAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSS 241
Query: 242 ISWTVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHP 301
ISWTVDGKGFFYSRYPAPKE G +DAGTET+ANLYHEVYYHFLG+DQSDDVLCWRD DHP
Sbjct: 242 ISWTVDGKGFFYSRYPAPKEVGTLDAGTETNANLYHEVYYHFLGTDQSDDVLCWRDQDHP 301
Query: 302 KYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDF 361
KYLFSA+VTDDGKYV++E +EGCDPVNKFYYC +SALPNGLEGF+GK+DLLPFTKLIDDF
Sbjct: 302 KYLFSASVTDDGKYVVMEIEEGCDPVNKFYYCKISALPNGLEGFKGKNDLLPFTKLIDDF 361
Query: 362 DAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQ 421
DAQYH IANDDTLFTFITNKNAPKYKLVRVDL DPTVWTELLPESEKDVLESA AVNGDQ
Sbjct: 362 DAQYHDIANDDTLFTFITNKNAPKYKLVRVDLNDPTVWTELLPESEKDVLESACAVNGDQ 421
Query: 422 MIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIY 481
MIVSYLSDVKYVLQIRDLKSGSLLH+LPIDIG+V GISARREDSLIFIGF+SFLTPGIIY
Sbjct: 422 MIVSYLSDVKYVLQIRDLKSGSLLHQLPIDIGTVYGISARREDSLIFIGFSSFLTPGIIY 481
Query: 482 QCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPC 541
QCNLESG PD+K+FREI VPGFERSEFNV+QVFV S DGTNIPMFIVARKNIV DGSHPC
Sbjct: 482 QCNLESGTPDLKIFREIAVPGFERSEFNVDQVFVRSNDGTNIPMFIVARKNIVFDGSHPC 541
Query: 542 LLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFD 601
LLYGYGGFNIN+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSLAKKQNCFD
Sbjct: 542 LLYGYGGFNINLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLAKKQNCFD 601
Query: 602 DFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHK 661
DFIS AEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDMLRFHK
Sbjct: 602 DFISCAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDMLRFHK 661
Query: 662 FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRV 721
FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADHDDRV
Sbjct: 662 FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRLLQYPSTMLLTADHDDRV 721
Query: 722 VPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMA 781
VPLHSLKLLATMQY+LCTSLEKSPQTNPI+GRIECKAGHGAGRPT+KMIDEASDRYAFMA
Sbjct: 722 VPLHSLKLLATMQYILCTSLEKSPQTNPIVGRIECKAGHGAGRPTQKMIDEASDRYAFMA 781
Query: 782 KTLEATWID 787
K L ATWID
Sbjct: 782 KMLAATWID 783
BLAST of MS021814 vs. ExPASy TrEMBL
Match:
A0A6J1FKH9 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111446264 PE=3 SV=1)
HSP 1 Score: 1421.0 bits (3677), Expect = 0.0e+00
Identity = 690/793 (87.01%), Postives = 735/793 (92.69%), Query Frame = 0
Query: 6 YTYIPLSSSFSPLFAPRLRLQPFRLLQVSSIP--LSAFP-------SSSSFK--FLSSSR 65
++++ SFSPLFAPRL+L RLLQVSS+P LSA P SSSS + S SR
Sbjct: 5 WSHLVAPLSFSPLFAPRLQL--LRLLQVSSVPSFLSALPPSYSTPSSSSSLRPSSFSPSR 64
Query: 66 PNMGSLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTE 125
MGSLSAL PL YPT+RRD+S+V+DYHGV+IADPYRWLEDPDA+EVKEFVQKQV LTE
Sbjct: 65 RRMGSLSALHEPLRYPTSRRDDSVVEDYHGVRIADPYRWLEDPDADEVKEFVQKQVTLTE 124
Query: 126 SVLQKCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPA 185
SVLQKC++REKLRAKIT+LFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQ SL GEP
Sbjct: 125 SVLQKCDTREKLRAKITQLFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQASLHGEPE 184
Query: 186 VLLDPNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWV 245
VLLDPNALSEDGTVSLS+LS+SKDAKYMAYGLSSSGSDWVTIKVMR+DDKKTEPDTLSWV
Sbjct: 185 VLLDPNALSEDGTVSLSSLSISKDAKYMAYGLSSSGSDWVTIKVMRIDDKKTEPDTLSWV 244
Query: 246 KFSGISWTVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRD 305
KFS ISWTVDGKGFFYSRYPAP+E G +DAGTET+ANLYHE+YYHFLGSDQ DDVLCWRD
Sbjct: 245 KFSSISWTVDGKGFFYSRYPAPREVGTLDAGTETNANLYHELYYHFLGSDQCDDVLCWRD 304
Query: 306 PDHPKYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKL 365
DHPKYLFS VT+DGKYVL+E EGCDPVNKFYYC++S LPNGLEGFR K DLLPFTKL
Sbjct: 305 QDHPKYLFSGRVTEDGKYVLMEISEGCDPVNKFYYCNISELPNGLEGFREKKDLLPFTKL 364
Query: 366 IDDFDAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAV 425
IDDFDAQYHAIANDD+LFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESA AV
Sbjct: 365 IDDFDAQYHAIANDDSLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESACAV 424
Query: 426 NGDQMIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTP 485
NGDQ+IVSYLSDVKYVLQIRDLKSGSLLH+LPIDIGSV GISARREDSL+FIGFTSFLTP
Sbjct: 425 NGDQVIVSYLSDVKYVLQIRDLKSGSLLHQLPIDIGSVYGISARREDSLVFIGFTSFLTP 484
Query: 486 GIIYQCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDG 545
GIIYQCNLE+G PDMK+FREI VPGFERSEF V+QVFV SKDG IPMFIVARKNI LDG
Sbjct: 485 GIIYQCNLETGTPDMKIFREIAVPGFERSEFEVDQVFVSSKDGAKIPMFIVARKNIALDG 544
Query: 546 SHPCLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQ 605
SHPCLLYGYGGFN+N+TP FSVSR VLARHLGA+FCIANIRGGGEYGEEWHKAGSL+KKQ
Sbjct: 545 SHPCLLYGYGGFNVNLTPYFSVSRTVLARHLGAVFCIANIRGGGEYGEEWHKAGSLSKKQ 604
Query: 606 NCFDDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDML 665
NCFDDFISSAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDML
Sbjct: 605 NCFDDFISSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDML 664
Query: 666 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADH 725
RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADH
Sbjct: 665 RFHKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRPLQYPSTMLLTADH 724
Query: 726 DDRVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRY 785
DDRVVPLHSLKLLATMQY+LCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRY
Sbjct: 725 DDRVVPLHSLKLLATMQYILCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRY 784
Query: 786 AFMAKTLEATWID 787
AFMAK L ATWID
Sbjct: 785 AFMAKMLSATWID 795
BLAST of MS021814 vs. ExPASy TrEMBL
Match:
A0A0A0L188 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_4G652900 PE=3 SV=1)
HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 685/789 (86.82%), Postives = 733/789 (92.90%), Query Frame = 0
Query: 2 LAFRYTY---IPLSSSFSPLFAPRLRLQPFRLLQVSSIPLSAFPSSSSFKFLSSSRPNMG 61
++FR+ + IPL SFSPLF P RL P L +SS P SSSS F + S MG
Sbjct: 2 VSFRFAFPYIIPL--SFSPLFPP--RLPPSSLSSLSS-PFPFLRSSSS--FFNHSPRRMG 61
Query: 62 SLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQ 121
SLSAL+ P YPTARRD+S+VDDYHG QI DPYRWLEDPDA+EVKEFV+KQVKLTESVLQ
Sbjct: 62 SLSALIDPFLYPTARRDDSVVDDYHGCQIVDPYRWLEDPDADEVKEFVEKQVKLTESVLQ 121
Query: 122 KCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLD 181
KC++REKLRAKITE FDHPRY+PPFKRGNKYFYFHNTGLQAQ++LYVQDSLDGEP VLLD
Sbjct: 122 KCDTREKLRAKITEQFDHPRYDPPFKRGNKYFYFHNTGLQAQNILYVQDSLDGEPEVLLD 181
Query: 182 PNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSG 241
PNALSEDGTVSLS LSVSKDAKY+AYGLSSSGSDWV IKVMR+DDKK EPDTLSWVKFS
Sbjct: 182 PNALSEDGTVSLSNLSVSKDAKYLAYGLSSSGSDWVKIKVMRIDDKKAEPDTLSWVKFSS 241
Query: 242 ISWTVDGKGFFYSRYPAPKEAG-VDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHP 301
ISWTVDGKGFFYSRYPAPKE G +DAGTET+ANLYHE+YYHFLG+DQSDDVLCWRD DHP
Sbjct: 242 ISWTVDGKGFFYSRYPAPKEVGTLDAGTETNANLYHELYYHFLGTDQSDDVLCWRDQDHP 301
Query: 302 KYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDF 361
KYLFSA+VTDDGKYVL+ +EGCDPVNKFYYC++SALPNGLEGF+GK+DLLPFTKLIDDF
Sbjct: 302 KYLFSASVTDDGKYVLMPIEEGCDPVNKFYYCNISALPNGLEGFKGKNDLLPFTKLIDDF 361
Query: 362 DAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQ 421
DAQY+AIANDDTLFTFITNKNAPKYKLVRVDL DPTVWTELLPES+KDVLESA AVNGDQ
Sbjct: 362 DAQYYAIANDDTLFTFITNKNAPKYKLVRVDLNDPTVWTELLPESDKDVLESACAVNGDQ 421
Query: 422 MIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIY 481
MIVSYLSDVKYVLQIRDLKSGSLLH+LPIDIG+V+GISARREDSLIFIGFTSFLTPGIIY
Sbjct: 422 MIVSYLSDVKYVLQIRDLKSGSLLHQLPIDIGTVNGISARREDSLIFIGFTSFLTPGIIY 481
Query: 482 QCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPC 541
QCNLESG PD+K+FRE VVPGFERS FNV+QVFV SKDGTNIPMF+VARKNIV DGSHPC
Sbjct: 482 QCNLESGTPDLKIFRETVVPGFERSAFNVDQVFVRSKDGTNIPMFVVARKNIVFDGSHPC 541
Query: 542 LLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFD 601
LLYGYGGFNI++TPSFSVSR VL RHLGA+FC+ANIRGGGEYGEEWHKAGSLAKKQNCFD
Sbjct: 542 LLYGYGGFNISLTPSFSVSRTVLMRHLGAVFCVANIRGGGEYGEEWHKAGSLAKKQNCFD 601
Query: 602 DFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHK 661
DFISSAEYLISAGYT+PSKLCIEGGSNGGLLVGACINQRPDL+GCALAHVGVMDMLRFHK
Sbjct: 602 DFISSAEYLISAGYTQPSKLCIEGGSNGGLLVGACINQRPDLFGCALAHVGVMDMLRFHK 661
Query: 662 FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRV 721
FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNV+RPWEQHPDR +QYP+TMLLTADHDDRV
Sbjct: 662 FTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVKRPWEQHPDRLLQYPSTMLLTADHDDRV 721
Query: 722 VPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMA 781
VPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPT+KMIDEASDRYAFMA
Sbjct: 722 VPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTQKMIDEASDRYAFMA 781
Query: 782 KTLEATWID 787
L ATWID
Sbjct: 782 MMLAATWID 783
BLAST of MS021814 vs. TAIR 10
Match:
AT1G76140.1 (Prolyl oligopeptidase family protein )
HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 568/776 (73.20%), Postives = 660/776 (85.05%), Query Frame = 0
Query: 17 PLFAPRLRLQPFRLLQVSSIPL-----SAFPSSSSFKFLSSSRPNMGSLSALLHPLHYPT 76
P RLR+ R +SS L S P+S S + SS MGS S L YP
Sbjct: 20 PTITRRLRINILRQSPLSSSLLLNETFSNRPNSVSRRCYCSSSAIMGSSSVFGEQLQYPA 79
Query: 77 ARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRAKIT 136
RRD+S+VDDYHGV+I DPYRWLEDPDAEEVKEFVQ QVKLT+SVL+KCE++EKLR IT
Sbjct: 80 TRRDDSVVDDYHGVKIGDPYRWLEDPDAEEVKEFVQSQVKLTDSVLEKCETKEKLRQNIT 139
Query: 137 ELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTVSLS 196
+L DHPRY+ PF++G+KYFYFHNTGLQAQSVLY+QD+LD EP VLLDPN LS+DGTV+L+
Sbjct: 140 KLIDHPRYDSPFRQGDKYFYFHNTGLQAQSVLYMQDNLDAEPEVLLDPNTLSDDGTVALN 199
Query: 197 ALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGFFYS 256
SVS+DAKY+AYGLSSSGSDWVTIK+M+++DKK EPDTLSWVKF+GI+WT D KGFFY
Sbjct: 200 TFSVSEDAKYLAYGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYG 259
Query: 257 RYPAPKEA-GVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDDGK 316
RYPAPKE +DAGTET++NLYHE+YYHF+G+DQS D+LCWRD ++PKY+F A VTDDGK
Sbjct: 260 RYPAPKEGEDIDAGTETNSNLYHELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGK 319
Query: 317 YVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDDTL 376
Y+++ E CDPVNK YYCDM++L GLE FRG S LPF KL+D FDAQY I+ND+TL
Sbjct: 320 YLIMSIGESCDPVNKLYYCDMTSLSGGLESFRGSSSFLPFIKLVDTFDAQYSVISNDETL 379
Query: 377 FTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIVSYLSDVKYVL 436
FTF+TNK+APKYKLVRVDLK+P WT+++ E EKDVL SA AVNG+ ++ Y+SDVK++L
Sbjct: 380 FTFLTNKDAPKYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHIL 439
Query: 437 QIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIPDMKV 496
QIRDLKSGSLLH+LP+DIGSVS +SARR+D+ F FTSFLTPG+IY+C+L + P++KV
Sbjct: 440 QIRDLKSGSLLHQLPLDIGSVSDVSARRKDNTFFFSFTSFLTPGVIYKCDLANESPEVKV 499
Query: 497 FREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFNINIT 556
FRE+ VPGF+R F QVF SKDGT IPMFIVA+K+I LDGSHPCLLY YGGFNI+IT
Sbjct: 500 FREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISIT 559
Query: 557 PSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYLISAG 616
PSFS SRIVL++HLG +FC ANIRGGGEYGEEWHKAGSLAKKQNCFDDFIS AEYL+SAG
Sbjct: 560 PSFSASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQNCFDDFISGAEYLVSAG 619
Query: 617 YTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC 676
YT+PSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC
Sbjct: 620 YTQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC 679
Query: 677 SDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLLATMQ 736
S+NEEEF WLIKYSPLHNV+RPWEQ D VQYP+TMLLTADHDDRVVPLHSLKLLAT+Q
Sbjct: 680 SENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLATLQ 739
Query: 737 YVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWID 787
+VLCTSL+ SPQ NPIIGRIE KAGHGAGRPT+KMIDEA+DRY+FMAK + A+W +
Sbjct: 740 HVLCTSLDNSPQMNPIIGRIEVKAGHGAGRPTQKMIDEAADRYSFMAKMVNASWTE 795
BLAST of MS021814 vs. TAIR 10
Match:
AT1G76140.2 (Prolyl oligopeptidase family protein )
HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 566/776 (72.94%), Postives = 657/776 (84.66%), Query Frame = 0
Query: 17 PLFAPRLRLQPFRLLQVSSIPL-----SAFPSSSSFKFLSSSRPNMGSLSALLHPLHYPT 76
P RLR+ R +SS L S P+S S + SS MGS S L YP
Sbjct: 20 PTITRRLRINILRQSPLSSSLLLNETFSNRPNSVSRRCYCSSSAIMGSSSVFGEQLQYPA 79
Query: 77 ARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESVLQKCESREKLRAKIT 136
RRD+S+VDDYHGV+I DPYRWLEDPDAEEVKEFVQ QVKLT+SVL+KCE++EKLR IT
Sbjct: 80 TRRDDSVVDDYHGVKIGDPYRWLEDPDAEEVKEFVQSQVKLTDSVLEKCETKEKLRQNIT 139
Query: 137 ELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVLLDPNALSEDGTVSLS 196
+L DHPRY+ PF++G+KYFYFHNTGLQAQSVLY+QD+LD EP VLLDPN LS+DGTV+L+
Sbjct: 140 KLIDHPRYDSPFRQGDKYFYFHNTGLQAQSVLYMQDNLDAEPEVLLDPNTLSDDGTVALN 199
Query: 197 ALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGFFYS 256
SVS+DAKY+AYGLSSSGSDWVTIK+M+++DKK EPDTLSWVKF+GI+WT D KGFFY
Sbjct: 200 TFSVSEDAKYLAYGLSSSGSDWVTIKLMKIEDKKVEPDTLSWVKFTGITWTHDSKGFFYG 259
Query: 257 RYPAPKEA-GVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSATVTDDGK 316
RYPAPKE +DAGTET++NLYHE+YYHF+G+DQS D+LCWRD ++PKY+F A VTDDGK
Sbjct: 260 RYPAPKEGEDIDAGTETNSNLYHELYYHFIGTDQSQDILCWRDNENPKYMFGAEVTDDGK 319
Query: 317 YVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLIDDFDAQYHAIANDDTL 376
Y+++ E CDPVNK YYCDM++L GLE FRG S LPF KL+D FDAQY I+ND+TL
Sbjct: 320 YLIMSIGESCDPVNKLYYCDMTSLSGGLESFRGSSSFLPFIKLVDTFDAQYSVISNDETL 379
Query: 377 FTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNGDQMIVSYLSDVKYVL 436
FTF+TNK+APKYKLVRVDLK+P WT+++ E EKDVL SA AVNG+ ++ Y+SDVK++L
Sbjct: 380 FTFLTNKDAPKYKLVRVDLKEPNSWTDVVEEHEKDVLASACAVNGNHLVACYMSDVKHIL 439
Query: 437 QIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCNLESGIPDMKV 496
QIRDLKSGSLLH+LP+DIGSVS +SARR+D+ F FTSFLTPG+IY+C+L + P++KV
Sbjct: 440 QIRDLKSGSLLHQLPLDIGSVSDVSARRKDNTFFFSFTSFLTPGVIYKCDLANESPEVKV 499
Query: 497 FREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHPCLLYGYGGFNINIT 556
FRE+ VPGF+R F QVF SKDGT IPMFIVA+K+I LDGSHPCLLY YGGFNI+IT
Sbjct: 500 FREVTVPGFDREAFQAIQVFYPSKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISIT 559
Query: 557 PSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYLISAG 616
PSFS SRIVL++HLG +FC ANIRGGGEYGEEWHKAGSLAKKQNCFDDFIS AEYL+SAG
Sbjct: 560 PSFSASRIVLSKHLGVVFCFANIRGGGEYGEEWHKAGSLAKKQNCFDDFISGAEYLVSAG 619
Query: 617 YTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC 676
YT+PSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC
Sbjct: 620 YTQPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTSDYGC 679
Query: 677 SDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLLATMQ 736
S+NEEEF WLIKYSPLHNV+RPWEQ D VQYP+TMLLTADHDDRVVPLHSLKLLA
Sbjct: 680 SENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHSLKLLA--- 739
Query: 737 YVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAFMAKTLEATWID 787
+VLCTSL+ SPQ NPIIGRIE KAGHGAGRPT+KMIDEA+DRY+FMAK + A+W +
Sbjct: 740 HVLCTSLDNSPQMNPIIGRIEVKAGHGAGRPTQKMIDEAADRYSFMAKMVNASWTE 792
BLAST of MS021814 vs. TAIR 10
Match:
AT1G20380.1 (Prolyl oligopeptidase family protein )
HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 549/731 (75.10%), Postives = 646/731 (88.37%), Query Frame = 0
Query: 57 MGSLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEEVKEFVQKQVKLTESV 116
MGSL A L YPTARRDES+V+DYHGV+++DPYRWLEDPDAEEVKEFV+KQV+L++SV
Sbjct: 1 MGSLPAFEERLQYPTARRDESVVEDYHGVKVSDPYRWLEDPDAEEVKEFVEKQVQLSDSV 60
Query: 117 LQKCESREKLRAKITELFDHPRYEPPFKRGNKYFYFHNTGLQAQSVLYVQDSLDGEPAVL 176
L+ CE++EKL K T+ D+PR++ PFKRGN YFYFHN+GLQAQSVL+VQD L+ E +L
Sbjct: 61 LKSCETKEKLHDKFTKFIDYPRFDTPFKRGNSYFYFHNSGLQAQSVLHVQDDLESEAEIL 120
Query: 177 LDPNALSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEPDTLSWVKF 236
LDPN LS+DGTVSL+ LS+S+DAKY+AYGLSSSGSDWVTIKVM+++DKK EPD+LSWVKF
Sbjct: 121 LDPNTLSDDGTVSLNTLSISEDAKYLAYGLSSSGSDWVTIKVMKIEDKKVEPDSLSWVKF 180
Query: 237 SGISWTVDGKGFFYSRYPAPKEA-GVDAGTETDANLYHEVYYHFLGSDQSDDVLCWRDPD 296
SGI+WT DGKGFFYSRYPAP+E +DAGTET++NLYHE+YYHFLG+DQS+DVLCWRD D
Sbjct: 181 SGITWTHDGKGFFYSRYPAPREGEKIDAGTETNSNLYHELYYHFLGTDQSEDVLCWRDQD 240
Query: 297 HPKYLFSATVTDDGKYVLLETDEGCDPVNKFYYCDMSALPNGLEGFRGKSDLLPFTKLID 356
+PK++F + VTDDGKY+++ +EGCDPVNK Y+CD+S LP GLEGFRG + LLPF KLID
Sbjct: 241 NPKHMFGSKVTDDGKYLIMSIEEGCDPVNKVYHCDLSLLPKGLEGFRGSNTLLPFVKLID 300
Query: 357 DFDAQYHAIANDDTLFTFITNKNAPKYKLVRVDLKDPTVWTELLPESEKDVLESATAVNG 416
FDAQY AIAND+TLFTF+TNK+APKYK+VRVDLK+P+ WT+++ E EKDVL +A+AVNG
Sbjct: 301 TFDAQYIAIANDETLFTFLTNKDAPKYKVVRVDLKEPSSWTDVIAEHEKDVLSTASAVNG 360
Query: 417 DQMIVSYLSDVKYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGI 476
DQ++VSY+SDVK++LQIRDLKSGSLLH LP+DIGSV G+ ARR+D+ F FTSFLTPG+
Sbjct: 361 DQLVVSYMSDVKHILQIRDLKSGSLLHGLPVDIGSVCGVFARRKDTTFFFRFTSFLTPGV 420
Query: 477 IYQCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSH 536
IY C+L P++ VFREI VPGF+R+ F V QVF SKDGT+IPMFIVARK+I LDGSH
Sbjct: 421 IYICDLSHEAPEVTVFREIGVPGFDRTAFQVTQVFYPSKDGTDIPMFIVARKDIKLDGSH 480
Query: 537 PCLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNC 596
PCLLY YGGF+I++TP FS +RIVL RHLG +FC ANIRGGGEYGEEWHK+G+LA KQNC
Sbjct: 481 PCLLYAYGGFSISMTPFFSATRIVLGRHLGTVFCFANIRGGGEYGEEWHKSGALANKQNC 540
Query: 597 FDDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRF 656
FDDFIS AEYL+SAGYT+P KLCIEGGSNGG+LVGACINQRPDL+GCALAHVGVMDMLRF
Sbjct: 541 FDDFISGAEYLVSAGYTQPRKLCIEGGSNGGILVGACINQRPDLFGCALAHVGVMDMLRF 600
Query: 657 HKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADHDD 716
HKFTIGHAWTS++GCSD EEEF WLIKYSPLHNV+RPWEQ D F QYP+TMLLTADHDD
Sbjct: 601 HKFTIGHAWTSEFGCSDKEEEFHWLIKYSPLHNVKRPWEQKTDLFFQYPSTMLLTADHDD 660
Query: 717 RVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRYAF 776
RVVPLHS KLLATMQY L SLE SPQTNPII RIE KAGHGAGRPT+KMIDEA+DRY+F
Sbjct: 661 RVVPLHSYKLLATMQYELGLSLENSPQTNPIIARIEVKAGHGAGRPTQKMIDEAADRYSF 720
Query: 777 MAKTLEATWID 787
MAK ++A+WID
Sbjct: 721 MAKMVDASWID 731
BLAST of MS021814 vs. TAIR 10
Match:
AT1G50380.1 (Prolyl oligopeptidase family protein )
HSP 1 Score: 213.0 bits (541), Expect = 8.6e-55
Identity = 198/751 (26.36%), Postives = 327/751 (43.54%), Query Frame = 0
Query: 70 PTARRDESIVDDYHGVQIADPYRWLEDPDA--EEVKEFVQKQVKLTESVLQKCESRE-KL 129
P A++ E +++ + V++ D Y WL D ++ +++++ T+ V+ + E +L
Sbjct: 8 PVAKKVEHVMEMFGDVRV-DNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTKQFENQL 67
Query: 130 RAKITELFDHPRYEPPFKRGNKYFYFHN--------------TGLQAQSVLY--VQDSLD 189
A+I P ++G Y+Y N T +A+ +Y + D
Sbjct: 68 FAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEPSVYDTMPTGPD 127
Query: 190 GEPA-VLLDPNA-LSEDGTVSLSALSVSKDAKYMAYGLSSSGSDWVTIKVMRVDDKKTEP 249
P V+LD N E + A S D K +AY + G + T+ V+ + K
Sbjct: 128 APPEHVILDENTKAQEHDYYRIGAFKASPDHKLVAYAEDTKGDEIYTVNVIDSEALKPVG 187
Query: 250 DTLSWVKFSGISWTVDGKGFFYSRYPAPKEAGVDA---GTETDANLYHEVYYHFLGSDQS 309
L + S + W AG DA T + +V+ H LG++QS
Sbjct: 188 QQLKGLT-SYLEW-----------------AGNDALLYITMDEILRPDKVWLHKLGTEQS 247
Query: 310 DDVLCWRDPDHPKYLFSATVTDDGKYVLLETDEGCDPVNKFYY-CDMSALPNGLEGFRGK 369
DV + + D + ++ KY+ + ++ +F + D+S +GL
Sbjct: 248 SDVCLYHEKD-DMFSLELHASESHKYLFVASE---SKTTRFVFSLDVSKTQDGLR----- 307
Query: 370 SDLLPFTKLIDDFDAQYHAIANDDTLFTFITNKNAPKY--KLVRVDLKDPTVWTELLPES 429
T +D D+ N FI ++ Y +L+ + D + T LLP
Sbjct: 308 ----VLTPRVDGIDSSVSHRGN----HFFIQRRSTEFYNSELIACPVDDTSKTTVLLPHR 367
Query: 430 EKDVLESATAVNGDQMIVSYLSDVKYVLQIRDLKSGSLLHKLP-------ID-IGSVSGI 489
E ++ + + ++ + R G L L +D + SV
Sbjct: 368 ESVKIQEIQLFRDHLAVFERENGLQKITVHRLPAEGQPLEGLQGGRNVSFVDPVYSVDST 427
Query: 490 SARREDSLIFIGFTSFLTPGIIYQCNLESGIPDMKVFREIVVPGFERSEFNVNQVFVHSK 549
+ ++ + S TP +Y +++SG +K + V+ GF+ S + + +V +
Sbjct: 428 ESEFSSRVLRFKYCSMKTPPSVYDYDMDSGTSVVKKI-DTVLGGFDASNYVTERKWVAAS 487
Query: 550 DGTNIPMFIVARKNIV-LDGSHPCLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANI 609
DGT IPM IV K + LDGS P LLYGYG + I++ P F SR+ L G F IA++
Sbjct: 488 DGTQIPMSIVYNKKLAKLDGSDPLLLYGYGSYEISVDPYFKASRLSLLDR-GFTFVIAHV 547
Query: 610 RGGGEYGEEWHKAGSLAKKQNCFDDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACI 669
RGGGE G +W++ G L KK+N F DFI+ AE LI Y KLC+EG S GGLL+GA +
Sbjct: 548 RGGGEMGRQWYENGKLLKKKNTFTDFIACAERLIELKYCSKEKLCMEGRSAGGLLMGAVV 607
Query: 670 NQRPDLYGCALAHVGVMDMLRFHKFTIGHAWTS---DYGCSDNEEEFKWLIKYSPLHNVR 729
N RPDL+ +A V +D+L TS ++G EE + ++ YSP+ NV
Sbjct: 608 NMRPDLFKVVIAGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVT 667
Query: 730 RPWEQHPDRFVQYPATMLLTADHDDRVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRI 782
YP ++ +D RV+ K +A ++ E N ++ +
Sbjct: 668 AQ---------NYPNMLVTAGLNDPRVMYSEPGKWVAKLR-------EMKTDNNVLLFKC 704
BLAST of MS021814 vs. TAIR 10
Match:
AT1G69020.1 (Prolyl oligopeptidase family protein )
HSP 1 Score: 123.6 bits (309), Expect = 6.9e-28
Identity = 169/787 (21.47%), Postives = 291/787 (36.98%), Query Frame = 0
Query: 42 PSSSSFKFLSSSRPNMGSLSALLHPLHYPTARRDESIVDDYHGVQIADPYRWLEDPDAEE 101
P++ S S++ +G S+L P P + HG+ DP+ W+++ D +
Sbjct: 12 PATISSVLSFSTKCFVGRTSSLSVPTEAPPVPKKIPFAISSHGITRQDPFHWMKNTDDTD 71
Query: 102 VKEFVQKQVKLTESVLQKCES-REKLRAKITELFDHPRYEPPFKRG----------NKYF 161
+F++++ +++ + E+ R L +++ + PP + G K +
Sbjct: 72 FVDFLKRENSYSQAFMADTETLRRDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPKGKEY 131
Query: 162 YFHNTGLQAQSVLYVQDSLDG-EPAVLLDPNALSED-GTVSLSALSVSKDAKYMAYGLSS 221
L+ ++ G E V+LD N ++E G V + VS D Y+AY +
Sbjct: 132 PLLCRRLEKGKTNWLSGLFRGEEEEVVLDWNQIAEQFGYVHVGVCRVSPDHNYLAYTVDP 191
Query: 222 SGSDWVTIKVMRVDDKKTEPDTLSWVKFSGISWTVDGKGFFYSRYPAPKEAGVDAGTETD 281
G D +T+ V D+ P + DG+ VD T D
Sbjct: 192 EG-DGITL-FYTVTDENQRPHRVVVTNVES-----DGRDDAVVFTERDSSFCVDITTTKD 251
Query: 282 ANLYHEVYYHFLGSDQSDDVLCWRDPDHPKYLFSAT-VTDDGKYVLLETDEGCDPVNKFY 341
+ + ++ + + D P T G LE G F+
Sbjct: 252 GK-----FVTINSNSRTSSEVYIVNADKPMAGLQRTRERVPGVQCFLEHHNG------FF 311
Query: 342 YCDMSALPNGLEGFRGK--------------SDLLPFTKLIDDFDAQYHAIANDDTLFTF 401
Y ++ N + + G+ SD + DD Q + N D L +
Sbjct: 312 YILTNSPSNAISEWSGEGYYLTRCLVEEIEASDWQTVFRPDDDVVIQDMDMFN-DYLVLY 371
Query: 402 ITNKNAPKYKLVRVDLK-------DPTVWTELLPESEKDVLESATAVNGDQMIVSYLSDV 461
+ K P + + +K D W LP V + + LS
Sbjct: 372 LNKKGLPMLCSIDMPIKANTKHMDDLVPWYFPLPVDSCSVAPGSNHDFQSSIYRVVLSSP 431
Query: 462 KYVLQIRDLKSGSLLHKLPIDIGSVSGISARREDSLIFIGFTSFLTPGIIYQCN------ 521
I D L + G V S + +T+ + Q N
Sbjct: 432 VIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSKP-----WYTADRSTENNGQLNDRTSEG 491
Query: 522 ----LESGIPDMKVFREIVVPGFERSEFNVNQVFVHSKDGTNIPMFIVARKNIVLDGSHP 581
L+S +P + + V ER E V S DG +P+ I+ + P
Sbjct: 492 EDGQLDSRMPKWEDLSDTYV--CERQE-------VSSHDGVEVPLTILYSREAWKKSESP 551
Query: 582 CLLYGYGGFNINITPSFSVSRIVLARHLGAIFCIANIRGGGEYGEEWHKAGSLAKKQNCF 641
+L GYG + + S+ +R+ + G + A++RGGG WHK+G+ + KQN
Sbjct: 552 GMLIGYGAYGEVLDKSWCTNRLSMLDR-GWVIAFADVRGGGSGEFSWHKSGTRSLKQNSI 611
Query: 642 DDFISSAEYLISAGYTRPSKLCIEGGSNGGLLVGACINQRPDLYGCALAHVGVMDMLRF- 701
DFI SA+YL+ GY L G S G +L A +N P L+ + V +D+L
Sbjct: 612 QDFIYSAKYLVEKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAVILKVPFVDVLNTL 671
Query: 702 --HKFTIGHAWTSDYGCSDNEEEFKWLIKYSPLHNVRRPWEQHPDRFVQYPATMLLTADH 761
+ ++G DN+ +F ++ YSP +R+ V YP+ ++ T+ H
Sbjct: 672 SDPNLPLTLLDHEEFGNPDNQTDFGSILSYSPYDKIRKD--------VCYPSMLVTTSFH 731
Query: 762 DDRVVPLHSLKLLATMQYVLCTSLEKSPQTNPIIGRIECKAGHGAGRPTKKMIDEASDRY 781
D RV K +A ++ C ++ +I + GH +E + Y
Sbjct: 732 DSRVGVWEGAKWVAKIRDSTCHDCSRA-----VILKTNMNGGHFGEGGRYAQCEETAFDY 751
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P48147 | 2.0e-250 | 55.76 | Prolyl endopeptidase OS=Homo sapiens OX=9606 GN=PREP PE=1 SV=2 | [more] |
Q9QUR6 | 3.5e-250 | 56.05 | Prolyl endopeptidase OS=Mus musculus OX=10090 GN=Prep PE=1 SV=1 | [more] |
P23687 | 6.5e-249 | 55.63 | Prolyl endopeptidase OS=Sus scrofa OX=9823 GN=PREP PE=1 SV=1 | [more] |
O70196 | 1.1e-248 | 56.19 | Prolyl endopeptidase OS=Rattus norvegicus OX=10116 GN=Prep PE=1 SV=1 | [more] |
Q9XTA2 | 2.3e-246 | 55.35 | Prolyl endopeptidase OS=Bos taurus OX=9913 GN=PREP PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BZ76 | 0.0e+00 | 99.49 | Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111006037 PE=3 SV=1 | [more] |
A0A6J1J0P1 | 0.0e+00 | 87.91 | Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111480845 PE=3 SV=1 | [more] |
A0A1S3BV12 | 0.0e+00 | 87.58 | Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103493792 PE=3 SV=1 | [more] |
A0A6J1FKH9 | 0.0e+00 | 87.01 | Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111446264 PE=3 SV=1 | [more] |
A0A0A0L188 | 0.0e+00 | 86.82 | Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_4G652900 PE=3 SV=1 | [more] |