MS021414 (gene) Bitter gourd (TR) v1

Overview
NameMS021414
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
Locationscaffold358: 1811351 .. 1813280 (-)
RNA-Seq ExpressionMS021414
SyntenyMS021414
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCAAACAGCTTCCAAATGCATCTTCTTTTCCTTTCTGTCTCTCCTAGCCATCTGCTCTTCTGGTAAGCAGTTTCTCCTCGTCGAACCAGAACAGATCTAGCTCAGTTTCTCGTTTTCTGCTCTGATTTGGTCTTGGTTCCTGCGAGTTACAGCTTCTAAGTCGAAAGTTTTCATTTTTTTCCTTCGCGAATTGCGTTTTCTGTTTTAGCAGAAATTTTAAGGGGTTTTGTTGTTTAGCATTGGGTGAATCTGTTTTCTGCTTCATTTCCGTTCTGTTTCTGAATGCTTTTGTCATTTTTGCAGGTAGATTTGTTGGGTCGTATTTGGAAGTGGAAGAAAATGCTTCCATGGAGTTGAGATGGGAAGAAGGCCGGAACCGGAAACTCACTGAGCGTGATCGGAGAACAATGCACGACATGACCAACTCGCCGACGACAATTTTTCCGACAACCCCTGATACCTCCACACCGACAATCATCACCGTTCCGGCAACCAACCCTGTGACTGTCACTCCGTCGAGCCCGGCTGCGACGCCGGTGTCCGTTCCTCTAACGACGCCCATCTCAGTTCCGGCCAACTCTCCGTTACCGCTCACCAATCCGGTTGCGCCGCCAATTACAGTACCCGGAGCACAGCCCATAACGAACCCGGTGACCACTTATCCAGCTCCGTCAGGCGGCGTTCCGGTGGTAACACCCGTCACAAATCCAGTGCCGGTATCCCCTCCGGCAACGACGAACGCGCCGGCGATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCCGGCGCATCGGAGACGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGGAGCTGACTGTTCCCAAATCCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTCAAAACCACGCTTCTTTTGCGTTCAATAGCTATTTTCAGAAGAATCCTTCCCCTACAAGCTGCGATTTTGGAGGATCTGCCATGGTTACCAGCTCCAATCCAAGTAAGAGAGCATTCTCATTGTCTCTCAATTCCTGCCGATCCTCTATTTCTTCTCAAGTTAACCAATTTTTGTGTCTTTAACAGGCAGTGGCTCCTGCGTTTATCCATTATCATCCTCTTCTTCGACACCGGCATCGTTGACACCATCGGTTCCAACGCCGACAACGACAGCTCCGATCAATGTCCCGCCAACTACAGTTACAAACCCAACAACGTCTTCACCTGCAGGGACCGGCATGCCAGAGTAAGGATATGCTCTGTTCTCTTCTCAGGTTTATAAAAATTGAATCTTTTTGATACTTTCTTAATAACTTGAGAAGCATAGAAATGGACATGAGACACACCAATACATAGTGACAAAGTTAATTTCGAAAAATCTAGGATATGGACATGACAAGAACGTGTTTATTATAATATATATTATTTTTATACATTTCAAAGATTCCCCTGTTAAAAATTTGTTCTAATTTAAAATGCTTGTGCATCAAAATCTTCGAGATGGATTGGAGAAAATTTAACAAGAAGAAAATTTAAAGTTGATAATGCTCATCCTTCCTATGTATTTCACCCACGATATGTTAACTGTGTTTAACAAGTGTAATGCTCGTATTCAGTTTATTCAACTAGTTTTTCACACAAGTCTATTGTGCCTAACAAATGTCCAACATATATATCCATCCATTGTTCAAGTTGAAGTGTTGGTGGTTCATATAATAACCATAATCTTGGTGCAGAAATGGAACTCCTCCGACAGTCTTTAACACAGGCAACCCAGCTTCGAGTATGGGCAGCACAACAGGCTTTGGAACGGAGATCCCACCAAGCTCAAGCACCTCGATATCCATGGCGGCTGGATTGAGAGTGAGACCCTTTACTTGCTGCATCACTCTGACCATGTCCTTGATCACACGAAGAATAATTCTGGACTGG

mRNA sequence

ATGGCCCAAACAGCTTCCAAATGCATCTTCTTTTCCTTTCTGTCTCTCCTAGCCATCTGCTCTTCTGGTAGATTTGTTGGGTCGTATTTGGAAGTGGAAGAAAATGCTTCCATGGAGTTGAGATGGGAAGAAGGCCGGAACCGGAAACTCACTGAGCGTGATCGGAGAACAATGCACGACATGACCAACTCGCCGACGACAATTTTTCCGACAACCCCTGATACCTCCACACCGACAATCATCACCGTTCCGGCAACCAACCCTGTGACTGTCACTCCGTCGAGCCCGGCTGCGACGCCGGTGTCCGTTCCTCTAACGACGCCCATCTCAGTTCCGGCCAACTCTCCGTTACCGCTCACCAATCCGGTTGCGCCGCCAATTACAGTACCCGGAGCACAGCCCATAACGAACCCGGTGACCACTTATCCAGCTCCGTCAGGCGGCGTTCCGGTGGTAACACCCGTCACAAATCCAGTGCCGGTATCCCCTCCGGCAACGACGAACGCGCCGGCGATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCCGGCGCATCGGAGACGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGGAGCTGACTGTTCCCAAATCCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTCAAAACCACGCTTCTTTTGCGTTCAATAGCTATTTTCAGAAGAATCCTTCCCCTACAAGCTGCGATTTTGGAGGATCTGCCATGGTTACCAGCTCCAATCCAAGCAGTGGCTCCTGCGTTTATCCATTATCATCCTCTTCTTCGACACCGGCATCGTTGACACCATCGGTTCCAACGCCGACAACGACAGCTCCGATCAATGTCCCGCCAACTACAGTTACAAACCCAACAACGTCTTCACCTGCAGGGACCGGCATGCCAGAAAATGGAACTCCTCCGACAGTCTTTAACACAGGCAACCCAGCTTCGAGTATGGGCAGCACAACAGGCTTTGGAACGGAGATCCCACCAAGCTCAAGCACCTCGATATCCATGGCGGCTGGATTGAGAGTGAGACCCTTTACTTGCTGCATCACTCTGACCATGTCCTTGATCACACGAAGAATAATTCTGGACTGG

Coding sequence (CDS)

ATGGCCCAAACAGCTTCCAAATGCATCTTCTTTTCCTTTCTGTCTCTCCTAGCCATCTGCTCTTCTGGTAGATTTGTTGGGTCGTATTTGGAAGTGGAAGAAAATGCTTCCATGGAGTTGAGATGGGAAGAAGGCCGGAACCGGAAACTCACTGAGCGTGATCGGAGAACAATGCACGACATGACCAACTCGCCGACGACAATTTTTCCGACAACCCCTGATACCTCCACACCGACAATCATCACCGTTCCGGCAACCAACCCTGTGACTGTCACTCCGTCGAGCCCGGCTGCGACGCCGGTGTCCGTTCCTCTAACGACGCCCATCTCAGTTCCGGCCAACTCTCCGTTACCGCTCACCAATCCGGTTGCGCCGCCAATTACAGTACCCGGAGCACAGCCCATAACGAACCCGGTGACCACTTATCCAGCTCCGTCAGGCGGCGTTCCGGTGGTAACACCCGTCACAAATCCAGTGCCGGTATCCCCTCCGGCAACGACGAACGCGCCGGCGATTCCAGGACAGAGCTGGTGCGTGGCCAGGTCCGGCGCATCGGAGACGGCTCTTCAGTCGGCGTTGGACTACGCCTGTGGCACTGGAGGAGCTGACTGTTCCCAAATCCAGCAAGGTGGAAGCTGTTACAACCCAAATACACTTCAAAACCACGCTTCTTTTGCGTTCAATAGCTATTTTCAGAAGAATCCTTCCCCTACAAGCTGCGATTTTGGAGGATCTGCCATGGTTACCAGCTCCAATCCAAGCAGTGGCTCCTGCGTTTATCCATTATCATCCTCTTCTTCGACACCGGCATCGTTGACACCATCGGTTCCAACGCCGACAACGACAGCTCCGATCAATGTCCCGCCAACTACAGTTACAAACCCAACAACGTCTTCACCTGCAGGGACCGGCATGCCAGAAAATGGAACTCCTCCGACAGTCTTTAACACAGGCAACCCAGCTTCGAGTATGGGCAGCACAACAGGCTTTGGAACGGAGATCCCACCAAGCTCAAGCACCTCGATATCCATGGCGGCTGGATTGAGAGTGAGACCCTTTACTTGCTGCATCACTCTGACCATGTCCTTGATCACACGAAGAATAATTCTGGACTGG

Protein sequence

MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHDMTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLTNPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVPTPTTTAPINVPPTTVTNPTTSSPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCITLTMSLITRRIILDW
Homology
BLAST of MS021414 vs. NCBI nr
Match: XP_022968747.1 (mucin-2 [Cucurbita maxima])

HSP 1 Score: 554.7 bits (1428), Expect = 6.2e-154
Identity = 314/373 (84.18%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS LSLLAICSSGRF GS+L+V+ENAS EL W      K +E  R TMHD
Sbjct: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLDVQENASAELEW------KFSELVRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
            TNSPTT FPTTPDTSTPTIITVPATNPVTVTPSSPA+TPVS+PLTTPI+VPANSP+PLT
Sbjct: 61  ATNSPTTNFPTTPDTSTPTIITVPATNPVTVTPSSPASTPVSIPLTTPITVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PVVTPVTNPVPVSPPA TNAP IPGQSWCV 
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVV 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGT GADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPSPTSC
Sbjct: 181 RSGASEMALQSALDYACGT-GADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSV--PTPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSV  PTPTTTAPI V PT VTNP TS
Sbjct: 241 DFGGSAMVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENGTPP +FN  NP+SS+GS+TGFGTEIPPSSSTSISMAAGL  RPFT CI 
Sbjct: 301 SPVGTGMPENGTPPAIFNPDNPSSSIGSSTGFGTEIPPSSSTSISMAAGL--RPFTWCII 360

Query: 361 LTMSLITRRIILD 372
           +TMSLITRRIIL+
Sbjct: 361 MTMSLITRRIILE 363

BLAST of MS021414 vs. NCBI nr
Match: XP_022961450.1 (mucin-2 [Cucurbita moschata] >KAG6590512.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 553.1 bits (1424), Expect = 1.8e-153
Identity = 314/373 (84.18%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS LSLLAICSSGRF GS+L+V+ENAS EL W      K +E  R TMHD
Sbjct: 1   MAQTASKCFFFSVLSLLAICSSGRFGGSHLDVQENASAELEW------KFSELVRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
            T+SPTT FPTTPDTSTPTIITVPATNP+TVTPSSPA+TPVS+PLTTPI+VPANSP+PLT
Sbjct: 61  ATDSPTTNFPTTPDTSTPTIITVPATNPITVTPSSPASTPVSIPLTTPITVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PVVTPVTNPVPVSPPA TNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGT GADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPSPTSC
Sbjct: 181 RSGASEMALQSALDYACGT-GADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSV--PTPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSV  PTPTTTAPI V PT VTNP TS
Sbjct: 241 DFGGSAMVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENGTPP VFN  NPASS+GS+TGFGTEIPPSSSTSISMAAGL  RPFT CI 
Sbjct: 301 SPVGTGMPENGTPPAVFNPDNPASSIGSSTGFGTEIPPSSSTSISMAAGL--RPFTWCII 360

Query: 361 LTMSLITRRIILD 372
           +TMSLITRRI L+
Sbjct: 361 MTMSLITRRITLE 363

BLAST of MS021414 vs. NCBI nr
Match: XP_023516007.1 (mucin-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.2 bits (1419), Expect = 6.8e-153
Identity = 314/373 (84.18%), Postives = 334/373 (89.54%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS LSLLAI SSGRF GS+L+V+ENAS EL W      K +E  R TMHD
Sbjct: 1   MAQTASKCFFFSILSLLAISSSGRFGGSHLDVQENASAELEW------KFSELVRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
            T+SPTT FPTTPDTSTPTIITVPATNP+TVTPSSPA+TPVS+PLTTPI+VPANSP+PLT
Sbjct: 61  ATDSPTTNFPTTPDTSTPTIITVPATNPITVTPSSPASTPVSIPLTTPITVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PVVTPVTNPVPVSPPA TNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGT GADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPSPTSC
Sbjct: 181 RSGASEMALQSALDYACGT-GADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSV--PTPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSV  PTPTTTAPI V PT VTNP TS
Sbjct: 241 DFGGSAMVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENGTPP VFN  NPASS+GS+TGFGTEIPPSSSTSISMAAGL  RPFT CI 
Sbjct: 301 SPVGTGMPENGTPPAVFNPDNPASSIGSSTGFGTEIPPSSSTSISMAAGL--RPFTWCII 360

Query: 361 LTMSLITRRIILD 372
           +TMSLITRRI LD
Sbjct: 361 MTMSLITRRITLD 363

BLAST of MS021414 vs. NCBI nr
Match: XP_004135574.1 (mucin-5AC [Cucumis sativus])

HSP 1 Score: 549.3 bits (1414), Expect = 2.6e-152
Identity = 312/375 (83.20%), Postives = 331/375 (88.27%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS +SLLAICSSGRF GS+L VEENAS EL+W      K  E  R TMHD
Sbjct: 1   MAQTASKCFFFSIMSLLAICSSGRFGGSHLGVEENASSELKW------KFPEFGRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
                TT FPTTPDTSTPTIITVP+TNPVT+TPSSPAATPVS+PLTTP +VPANSP+PLT
Sbjct: 61  ----ATTNFPTTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PV+TP TNPVPVSPPATTNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGTGGADCSQIQQ GSCYNPNTL+NHASFAFNSYFQKNPS TSC
Sbjct: 181 RSGASEMALQSALDYACGTGGADCSQIQQSGSCYNPNTLENHASFAFNSYFQKNPSSTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVP--TPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSVP  TPTTTAPI V PTTVTNPTTS
Sbjct: 241 DFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENG+PP VFNT NPASS+GSTTGFGTEIPPSSSTSIS+AAGL  RPFTC I 
Sbjct: 301 SPVGTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISVAAGL--RPFTCFII 360

Query: 361 LTMSLITRRII-LDW 373
           LTMS IT RII LDW
Sbjct: 361 LTMSFITHRIITLDW 363

BLAST of MS021414 vs. NCBI nr
Match: XP_008450517.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 4 [Cucumis melo] >KAA0050964.1 glucan endo-1,3-beta-glucosidase 4 [Cucumis melo var. makuwa] >TYK10310.1 glucan endo-1,3-beta-glucosidase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 542.7 bits (1397), Expect = 2.4e-150
Identity = 306/371 (82.48%), Postives = 327/371 (88.14%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQ ASKC FFS +SLLAICSSGRF GS+L VEEN S+EL+W      K  E  R TMHD
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKW------KFIEFGRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
                TT FPTTPDTSTPTIITVP+TNPVT+TPSSPAATPVS+PLTTP +VPANSP+PLT
Sbjct: 61  ----ATTNFPTTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PV+TP TNPVPVSPPATTNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGTGGADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPS TSC
Sbjct: 181 RSGASEMALQSALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVP--TPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSVP  TPTTTAPI V PTTVTNPTTS
Sbjct: 241 DFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP G+GMPENG+PP  FNT NPASS+GSTTGFGTEIPPSSSTSIS+AAGL  RPFTC I 
Sbjct: 301 SPVGSGMPENGSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGL--RPFTCFII 359

Query: 361 LTMSLITRRII 370
           LTMS IT RII
Sbjct: 361 LTMSFITHRII 359

BLAST of MS021414 vs. ExPASy Swiss-Prot
Match: Q93V72 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.1e-20
Identity = 77/178 (43.26%), Postives = 106/178 (59.55%), Query Frame = 0

Query: 176 SWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNP 235
           ++C+ + G +E  LQ A+DYACG  GADC+QIQ  G+CY PNT++NH   A NSY+QK  
Sbjct: 20  AYCLCKEG-NEQVLQKAIDYACG-NGADCTQIQPTGACYQPNTVKNHCDVAVNSYYQKKA 79

Query: 236 SP-TSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVPTPTTTAPINVPPTTVTN 295
           S   +CDF G+A  +++ PS+ S     SSSS TP + TP+  TPT+  P    PTT T 
Sbjct: 80  SSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTSGTPTTGTPTTGT- 139

Query: 296 PTTSSPAG----TGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGL 349
           PTT +P      +G P  GTP T  NTG P +S G  T   + + P ++   + + GL
Sbjct: 140 PTTGTPTSGTPTSGFPNTGTPNTGTNTGMP-NSNGMPTSSSSSVFPGTTLGPTGSGGL 193

BLAST of MS021414 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 99.8 bits (247), Expect = 7.1e-20
Identity = 52/102 (50.98%), Postives = 70/102 (68.63%), Query Frame = 0

Query: 161 VSPPATTNAPAIPGQSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQ 220
           VS   T  A     Q++C+A  G     LQ+ALD+ACG G ++CS+IQ G SCY PN ++
Sbjct: 365 VSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVK 424

Query: 221 NHASFAFNSYFQKNPSPT-SCDFGGSAMVTSSNPSSGSCVYP 262
            HASFAFNSY+QK    + SCDF G AM+T+++PS GSC++P
Sbjct: 425 GHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIFP 466

BLAST of MS021414 vs. ExPASy Swiss-Prot
Match: Q9FZ86 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-19
Identity = 66/140 (47.14%), Postives = 86/140 (61.43%), Query Frame = 0

Query: 176 SWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQ-KN 235
           +WCV + G SE  LQ  LDYACG  GADC  I Q G C+NPNT+++H S+A NS+FQ K 
Sbjct: 20  TWCVCKEGLSEAMLQKTLDYACG-AGADCGPIHQTGPCFNPNTVKSHCSYAVNSFFQKKG 79

Query: 236 PSPTSCDFGGSAMVTSSNPSSGSCVYPLSSSSS---TPASLTPSVPTPTTTAPINVPPTT 295
            S  +CDF G+A  ++S+PS  +C +P S+S S   TP + TPS   PTTT   N  P T
Sbjct: 80  QSLGTCDFAGTATFSASDPSYTTCPFPASASGSGTTTPVTTTPSTRVPTTT---NTRPYT 139

Query: 296 VTNPTTSSPAGTGMPENGTP 312
           +   T S+  G G+P    P
Sbjct: 140 I---TPSTGGGLGIPSGINP 152

BLAST of MS021414 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 1.3e-18
Identity = 49/101 (48.51%), Postives = 66/101 (65.35%), Query Frame = 0

Query: 162 SPPATTNAPAIPGQSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQN 221
           S  + T +  +    WCV + GA+   LQ++LD+ACG  G DC  IQ GG+C+ PN + +
Sbjct: 349 SSSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACG-HGIDCGAIQPGGACFEPNNVVS 408

Query: 222 HASFAFNSYFQKNP-SPTSCDFGGSAMVTSSNPSSGSCVYP 262
           HA++A N YFQK+P  PT CDF  +A VTS NPS  +CVYP
Sbjct: 409 HAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of MS021414 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 3.0e-18
Identity = 74/188 (39.36%), Postives = 105/188 (55.85%), Query Frame = 0

Query: 176 SWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQ-KN 235
           SWCV ++G S+T LQ+ LDYACG  GADC+  +   SC+NP+ +++H ++A NS+FQ K 
Sbjct: 20  SWCVCKTGLSDTVLQATLDYACG-NGADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKKG 79

Query: 236 PSPTSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVPTPTTTAPINVPPTTVTN 295
            SP SC+F G+A  T+S+PS   C +P S+S S+ ++      TP TT P   P T    
Sbjct: 80  QSPGSCNFDGTATPTNSDPSYTGCAFPTSASGSSGSTTV----TPGTTNPKGSPTT---- 139

Query: 296 PTTSSPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFT 355
            TT   +GT  P +G P      GN   S G TTG G   P  ++ S + A     + F 
Sbjct: 140 -TTLPGSGTNSPYSGNPTNGVFGGN---STGGTTGTGIN-PDYTTDSSAFALKNSSKLFI 193

Query: 356 CCITLTMS 363
           C + +  S
Sbjct: 200 CLLLIASS 193

BLAST of MS021414 vs. ExPASy TrEMBL
Match: A0A6J1HUD2 (mucin-2 OS=Cucurbita maxima OX=3661 GN=LOC111467890 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 3.0e-154
Identity = 314/373 (84.18%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS LSLLAICSSGRF GS+L+V+ENAS EL W      K +E  R TMHD
Sbjct: 1   MAQTASKCFFFSILSLLAICSSGRFGGSHLDVQENASAELEW------KFSELVRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
            TNSPTT FPTTPDTSTPTIITVPATNPVTVTPSSPA+TPVS+PLTTPI+VPANSP+PLT
Sbjct: 61  ATNSPTTNFPTTPDTSTPTIITVPATNPVTVTPSSPASTPVSIPLTTPITVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PVVTPVTNPVPVSPPA TNAP IPGQSWCV 
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVV 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGT GADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPSPTSC
Sbjct: 181 RSGASEMALQSALDYACGT-GADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSV--PTPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSV  PTPTTTAPI V PT VTNP TS
Sbjct: 241 DFGGSAMVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENGTPP +FN  NP+SS+GS+TGFGTEIPPSSSTSISMAAGL  RPFT CI 
Sbjct: 301 SPVGTGMPENGTPPAIFNPDNPSSSIGSSTGFGTEIPPSSSTSISMAAGL--RPFTWCII 360

Query: 361 LTMSLITRRIILD 372
           +TMSLITRRIIL+
Sbjct: 361 MTMSLITRRIILE 363

BLAST of MS021414 vs. ExPASy TrEMBL
Match: A0A6J1HC94 (mucin-2 OS=Cucurbita moschata OX=3662 GN=LOC111462029 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 8.7e-154
Identity = 314/373 (84.18%), Postives = 335/373 (89.81%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS LSLLAICSSGRF GS+L+V+ENAS EL W      K +E  R TMHD
Sbjct: 1   MAQTASKCFFFSVLSLLAICSSGRFGGSHLDVQENASAELEW------KFSELVRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
            T+SPTT FPTTPDTSTPTIITVPATNP+TVTPSSPA+TPVS+PLTTPI+VPANSP+PLT
Sbjct: 61  ATDSPTTNFPTTPDTSTPTIITVPATNPITVTPSSPASTPVSIPLTTPITVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PVVTPVTNPVPVSPPA TNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGSPVVTPVTNPVPVSPPAMTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGT GADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPSPTSC
Sbjct: 181 RSGASEMALQSALDYACGT-GADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSPTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSV--PTPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSV  PTPTTTAPI V PT VTNP TS
Sbjct: 241 DFGGSAMVTNSNPSTGSCLYPSSSSSATPASMTPSVPTPTPTTTAPITVAPTIVTNP-TS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENGTPP VFN  NPASS+GS+TGFGTEIPPSSSTSISMAAGL  RPFT CI 
Sbjct: 301 SPVGTGMPENGTPPAVFNPDNPASSIGSSTGFGTEIPPSSSTSISMAAGL--RPFTWCII 360

Query: 361 LTMSLITRRIILD 372
           +TMSLITRRI L+
Sbjct: 361 MTMSLITRRITLE 363

BLAST of MS021414 vs. ExPASy TrEMBL
Match: A0A0A0M1B6 (Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus OX=3659 GN=Csa_1G560700 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.3e-152
Identity = 312/375 (83.20%), Postives = 331/375 (88.27%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQTASKC FFS +SLLAICSSGRF GS+L VEENAS EL+W      K  E  R TMHD
Sbjct: 1   MAQTASKCFFFSIMSLLAICSSGRFGGSHLGVEENASSELKW------KFPEFGRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
                TT FPTTPDTSTPTIITVP+TNPVT+TPSSPAATPVS+PLTTP +VPANSP+PLT
Sbjct: 61  ----ATTNFPTTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PV+TP TNPVPVSPPATTNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGTGGADCSQIQQ GSCYNPNTL+NHASFAFNSYFQKNPS TSC
Sbjct: 181 RSGASEMALQSALDYACGTGGADCSQIQQSGSCYNPNTLENHASFAFNSYFQKNPSSTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVP--TPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSVP  TPTTTAPI V PTTVTNPTTS
Sbjct: 241 DFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP GTGMPENG+PP VFNT NPASS+GSTTGFGTEIPPSSSTSIS+AAGL  RPFTC I 
Sbjct: 301 SPVGTGMPENGSPPGVFNTDNPASSIGSTTGFGTEIPPSSSTSISVAAGL--RPFTCFII 360

Query: 361 LTMSLITRRII-LDW 373
           LTMS IT RII LDW
Sbjct: 361 LTMSFITHRIITLDW 363

BLAST of MS021414 vs. ExPASy TrEMBL
Match: A0A5A7U6N8 (Glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004640 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.2e-150
Identity = 306/371 (82.48%), Postives = 327/371 (88.14%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQ ASKC FFS +SLLAICSSGRF GS+L VEEN S+EL+W      K  E  R TMHD
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKW------KFIEFGRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
                TT FPTTPDTSTPTIITVP+TNPVT+TPSSPAATPVS+PLTTP +VPANSP+PLT
Sbjct: 61  ----ATTNFPTTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PV+TP TNPVPVSPPATTNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGTGGADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPS TSC
Sbjct: 181 RSGASEMALQSALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVP--TPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSVP  TPTTTAPI V PTTVTNPTTS
Sbjct: 241 DFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP G+GMPENG+PP  FNT NPASS+GSTTGFGTEIPPSSSTSIS+AAGL  RPFTC I 
Sbjct: 301 SPVGSGMPENGSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGL--RPFTCFII 359

Query: 361 LTMSLITRRII 370
           LTMS IT RII
Sbjct: 361 LTMSFITHRII 359

BLAST of MS021414 vs. ExPASy TrEMBL
Match: A0A1S3BQE9 (glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo OX=3656 GN=LOC103492098 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.2e-150
Identity = 306/371 (82.48%), Postives = 327/371 (88.14%), Query Frame = 0

Query: 1   MAQTASKCIFFSFLSLLAICSSGRFVGSYLEVEENASMELRWEEGRNRKLTERDRRTMHD 60
           MAQ ASKC FFS +SLLAICSSGRF GS+L VEEN S+EL+W      K  E  R TMHD
Sbjct: 1   MAQAASKCFFFSIMSLLAICSSGRFGGSHLGVEENTSLELKW------KFIEFGRTTMHD 60

Query: 61  MTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLPLT 120
                TT FPTTPDTSTPTIITVP+TNPVT+TPSSPAATPVS+PLTTP +VPANSP+PLT
Sbjct: 61  ----ATTNFPTTPDTSTPTIITVPSTNPVTITPSSPAATPVSIPLTTPFTVPANSPVPLT 120

Query: 121 NPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPATTNAPAIPGQSWCVA 180
           NPVAPP+TVPGAQPITNPVTTYPAPSGG PV+TP TNPVPVSPPATTNAP IPGQSWCVA
Sbjct: 121 NPVAPPVTVPGAQPITNPVTTYPAPSGGAPVLTPPTNPVPVSPPATTNAPVIPGQSWCVA 180

Query: 181 RSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNPSPTSC 240
           RSGASE ALQSALDYACGTGGADCSQIQQGGSCYNPNTL+NHASFAFNSYFQKNPS TSC
Sbjct: 181 RSGASEMALQSALDYACGTGGADCSQIQQGGSCYNPNTLENHASFAFNSYFQKNPSSTSC 240

Query: 241 DFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVP--TPTTTAPINVPPTTVTNPTTS 300
           DFGGSAMVT+SNPS+GSC+YP SSSS+TPAS+TPSVP  TPTTTAPI V PTTVTNPTTS
Sbjct: 241 DFGGSAMVTNSNPSTGSCIYPSSSSSATPASMTPSVPTQTPTTTAPITVSPTTVTNPTTS 300

Query: 301 SPAGTGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGLRVRPFTCCIT 360
           SP G+GMPENG+PP  FNT NPASS+GSTTGFGTEIPPSSSTSIS+AAGL  RPFTC I 
Sbjct: 301 SPVGSGMPENGSPPGAFNTDNPASSIGSTTGFGTEIPPSSSTSISIAAGL--RPFTCFII 359

Query: 361 LTMSLITRRII 370
           LTMS IT RII
Sbjct: 361 LTMSFITHRII 359

BLAST of MS021414 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.5e-54
Identity = 149/296 (50.34%), Postives = 190/296 (64.19%), Query Frame = 0

Query: 57  TMHDMTNSPTTIFPTTPDTSTPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSP 116
           T HD+ N P T+FPT P T+TPT  T P   PVT+TP++PA T   VP  T I  P  +P
Sbjct: 26  THHDVLN-PPTVFPTNP-TTTPT-ATFP---PVTITPTNPATTVPIVPPVTTIPPPTLTP 85

Query: 117 LPLTNPVAPPITVPGAQPITNPVTTYP--APSGGVPVVTPVTNPVPVSPPATTNAPAIPG 176
            P+     P +T P   P+TNPVT YP   PSG VPV  PV     V+PP  +N+P++ G
Sbjct: 86  PPVITIPPPTLTPPVTNPVTNPVTQYPPTQPSGTVPVPVPV-----VAPPVVSNSPSVSG 145

Query: 177 QSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKN 236
           QSWCVA+ GAS+ +LQ ALDYACG   ADCSQ+QQGG+CY+P +LQ+HASFAFNSY+QKN
Sbjct: 146 QSWCVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKN 205

Query: 237 PSPTSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVPTPTTTAPINVPPTTVTN 296
           PSP SCDFGG+A + ++NPS+GSC+Y   SS+STP +   + PTP+T   +N PP T   
Sbjct: 206 PSPQSCDFGGAASLVNTNPSTGSCIYQTGSSTSTPMTAGTTTPTPSTQT-VNQPPVT--- 265

Query: 297 PTTSSPAGTGMPENGTPPTVFNTGNPA-------SSMGSTTGFGTEIPPSSSTSIS 344
            T   P G G+   GTPP +FN  NP        SS G   G+G +  P+ +   S
Sbjct: 266 STPIIPTGGGIIGVGTPPAIFNPANPTSNTLNNPSSGGLAGGYGFDGSPNENNPTS 304

BLAST of MS021414 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-28
Identity = 86/200 (43.00%), Postives = 118/200 (59.00%), Query Frame = 0

Query: 106 TTPISVPANSPLPLTNPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPA 165
           T  I+ P      +T P+A         P T P T  P        V       P++ P+
Sbjct: 21  TKAITEPIEEEKDITTPLA-------TNPTTTPTTVVPNSDSDASAVA----TTPLTIPS 80

Query: 166 TTNAPAIPG-QSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHAS 225
           + +  A PG QSWCVAR   ++ ALQ+ALDYACG GGADCS+IQ+GG+CYNPN+L+ HAS
Sbjct: 81  SPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHAS 140

Query: 226 FAFNSYFQKNPSPTSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLT----------- 285
           FAFNSY+QKNP P+SC+F G+A+  S++PS GSC +P +S+S +  ++T           
Sbjct: 141 FAFNSYYQKNPIPSSCNFDGTAITISADPSLGSCHFPSTSTSESILNVTSEDGLGLFGRI 200

Query: 286 PSVPTPTTTAPINVPPTTVT 294
           PS PTP   A  +   T ++
Sbjct: 201 PSHPTPKPEASTSSSRTLIS 209

BLAST of MS021414 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.1e-27
Identity = 72/150 (48.00%), Postives = 95/150 (63.33%), Query Frame = 0

Query: 106 TTPISVPANSPLPLTNPVAPPITVPGAQPITNPVTTYPAPSGGVPVVTPVTNPVPVSPPA 165
           T  I+ P      +T P+A         P T P T  P        V       P++ P+
Sbjct: 21  TKAITEPIEEEKDITTPLA-------TNPTTTPTTVVPNSDSDASAVA----TTPLTIPS 80

Query: 166 TTNAPAIPG-QSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHAS 225
           + +  A PG QSWCVAR   ++ ALQ+ALDYACG GGADCS+IQ+GG+CYNPN+L+ HAS
Sbjct: 81  SPHGVAYPGDQSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHAS 140

Query: 226 FAFNSYFQKNPSPTSCDFGGSAMVTSSNPS 255
           FAFNSY+QKNP P+SC+F G+A+  S++PS
Sbjct: 141 FAFNSYYQKNPIPSSCNFDGTAITISADPS 159

BLAST of MS021414 vs. TAIR 10
Match: AT1G69295.1 (plasmodesmata callose-binding protein 4 )

HSP 1 Score: 102.4 bits (254), Expect = 7.8e-22
Identity = 77/178 (43.26%), Postives = 106/178 (59.55%), Query Frame = 0

Query: 176 SWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYNPNTLQNHASFAFNSYFQKNP 235
           ++C+ + G +E  LQ A+DYACG  GADC+QIQ  G+CY PNT++NH   A NSY+QK  
Sbjct: 20  AYCLCKEG-NEQVLQKAIDYACG-NGADCTQIQPTGACYQPNTVKNHCDVAVNSYYQKKA 79

Query: 236 SP-TSCDFGGSAMVTSSNPSSGSCVYPLSSSSSTPASLTPSVPTPTTTAPINVPPTTVTN 295
           S   +CDF G+A  +++ PS+ S     SSSS TP + TP+  TPT+  P    PTT T 
Sbjct: 80  SSGATCDFNGAASPSTTPPSTASNCLTGSSSSGTPTTGTPTTGTPTSGTPTTGTPTTGT- 139

Query: 296 PTTSSPAG----TGMPENGTPPTVFNTGNPASSMGSTTGFGTEIPPSSSTSISMAAGL 349
           PTT +P      +G P  GTP T  NTG P +S G  T   + + P ++   + + GL
Sbjct: 140 PTTGTPTSGTPTSGFPNTGTPNTGTNTGMP-NSNGMPTSSSSSVFPGTTLGPTGSGGL 193

BLAST of MS021414 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 3.0e-21
Identity = 100/284 (35.21%), Postives = 139/284 (48.94%), Query Frame = 0

Query: 76  STPTIITVPATNPVTVTPSSPAATPVSVPLTTPISVPANSPLP-LTNPVAPPITVPGAQP 135
           ST  ++ +  T+P+ V           +P+  P +   ++  P +T P  P +T PG   
Sbjct: 11  STIMLLCLLYTSPLDVIAQGQGGQG-DIPVVNPTAPGGSTTTPTITQPSPPSLTFPGP-- 70

Query: 136 ITNPVTTYP-----APSGGVPVVTPVTNP---------------VPVSPPA--------- 195
            T P   YP      P+GG P +   T P                P              
Sbjct: 71  -TTPTGGYPPLDGTTPTGGYPPLYGTTPPGGGDVGGGGGGYGGGTPGGGGGGGGDTGAGA 130

Query: 196 ----------TTNAPAIPGQSWCVARSGASETALQSALDYACGTGGADCSQIQQGGSCYN 255
                     T     +    WC+A++ AS T+LQ ALDYACG GGADC QIQQG +CY 
Sbjct: 131 GGGGYGGGGDTGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYE 190

Query: 256 PNTLQNHASFAFNSYFQKNPSPTSCDFGGSAMVTSSNPSSGSCVYPLSS---SSSTPASL 311
           PNT+++HASFAFNSY+QK+P   SC+FGG+A +TS++PS GSC +  SS   S+S P+ +
Sbjct: 191 PNTIRDHASFAFNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHFSSSSGTVSTSPPSQM 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022968747.16.2e-15484.18mucin-2 [Cucurbita maxima][more]
XP_022961450.11.8e-15384.18mucin-2 [Cucurbita moschata] >KAG6590512.1 PLASMODESMATA CALLOSE-BINDING PROTEIN... [more]
XP_023516007.16.8e-15384.18mucin-2 [Cucurbita pepo subsp. pepo][more]
XP_004135574.12.6e-15283.20mucin-5AC [Cucumis sativus][more]
XP_008450517.12.4e-15082.48PREDICTED: glucan endo-1,3-beta-glucosidase 4 [Cucumis melo] >KAA0050964.1 gluca... [more]
Match NameE-valueIdentityDescription
Q93V721.1e-2043.26PLASMODESMATA CALLOSE-BINDING PROTEIN 4 OS=Arabidopsis thaliana OX=3702 GN=PDCB4... [more]
O653997.1e-2050.98Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9FZ861.6e-1947.14PLASMODESMATA CALLOSE-BINDING PROTEIN 3 OS=Arabidopsis thaliana OX=3702 GN=PDCB3... [more]
Q9M0691.3e-1848.51Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q9FNQ23.0e-1839.36PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1... [more]
Match NameE-valueIdentityDescription
A0A6J1HUD23.0e-15484.18mucin-2 OS=Cucurbita maxima OX=3661 GN=LOC111467890 PE=4 SV=1[more]
A0A6J1HC948.7e-15484.18mucin-2 OS=Cucurbita moschata OX=3662 GN=LOC111462029 PE=4 SV=1[more]
A0A0A0M1B61.3e-15283.20Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A5A7U6N81.2e-15082.48Glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BQE91.2e-15082.48glucan endo-1,3-beta-glucosidase 4 OS=Cucumis melo OX=3656 GN=LOC103492098 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G09460.13.5e-5450.34Carbohydrate-binding X8 domain superfamily protein [more]
AT2G30933.12.5e-2843.00Carbohydrate-binding X8 domain superfamily protein [more]
AT2G30933.22.1e-2748.00Carbohydrate-binding X8 domain superfamily protein [more]
AT1G69295.17.8e-2243.26plasmodesmata callose-binding protein 4 [more]
AT1G29380.13.0e-2135.21Carbohydrate-binding X8 domain superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01217PRICHEXTENSNcoord: 118..139
score: 34.55
coord: 143..159
score: 35.29
coord: 269..281
score: 53.85
coord: 88..104
score: 40.0
coord: 160..177
score: 28.89
coord: 72..84
score: 44.62
NoneNo IPR availableGENE3D1.20.58.1040coord: 172..271
e-value: 9.1E-12
score: 47.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..338
NoneNo IPR availablePANTHERPTHR31044:SF97CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEINcoord: 9..344
IPR012946X8 domainSMARTSM00768X8_clscoord: 176..260
e-value: 3.0E-49
score: 179.6
IPR012946X8 domainPFAMPF07983X8coord: 177..247
e-value: 1.1E-21
score: 77.2
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 9..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021414.1MS021414.1mRNA