MS021318 (gene) Bitter gourd (TR) v1

BLAST of MS021318 vs. NCBI nr
Match: XP_022139640.1 (lanC-like protein GCR2 [Momordica charantia])

HSP 1 Score: 844.7 bits (2181), Expect = 3.3e-241
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR
Sbjct: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS
Sbjct: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN
Sbjct: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN
Sbjct: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300
           VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF
Sbjct: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300

Query: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360
           GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF
Sbjct: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360

Query: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL
Sbjct: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 410

BLAST of MS021318 vs. NCBI nr
Match: XP_022981295.1 (lanC-like protein GCR2 [Cucurbita maxima])

HSP 1 Score: 699.1 bits (1803), Expect = 2.2e-197
Identity = 337/414 (81.40%), Postives = 374/414 (90.34%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDS----DALTLTELLSLPYAALSDHFRKAAFDLKE 60
           MADRFFPNQMP FVAEAPA+ELA SDS    D LT ++LLSLP+AALS  F + A DLKE
Sbjct: 1   MADRFFPNQMPHFVAEAPADELAPSDSDIAGDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120
           TVVR  WT  E +P+D+TLYTGALGTAFLALKSY+VSNNE DLKLCSEIV AC+++S DS
Sbjct: 61  TVVRGMWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETLSGDS 120

Query: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180
           RRVSF+CGRAGVCALGAVAAKLAN+GR V HYLEKF++IK P DLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI  +TIS TIMR+VVDEVI+AGRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRAVVDEVIKAGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300
           VLMN++L+PDE EDVK+T+RYMIK+RFPSGN+ SSEGN+SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFHSSEGNDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360
            E+FGD +FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FACFLHQNA+ LISEGKM SGDRP+S+FEGIGGMAYLFFDM EPS ARFP+YEL
Sbjct: 361 FACFLHQNAQMLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of MS021318 vs. NCBI nr
Match: XP_004141231.2 (lanC-like protein GCR2 [Cucumis sativus] >KAE8649848.1 hypothetical protein Csa_012513 [Cucumis sativus])

HSP 1 Score: 694.9 bits (1792), Expect = 4.2e-196
Identity = 338/412 (82.04%), Postives = 369/412 (89.56%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTL--TELLSLPYAALSDHFRKAAFDLKETV 60
           MA+RFFPNQMP F AEAP +ELA+S S + TL  TELLSLP AALSD  R+AA +LKETV
Sbjct: 1   MAERFFPNQMPRFPAEAPPDELASSHSYSATLIPTELLSLPDAALSDRLRQAALNLKETV 60

Query: 61  VRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRR 120
           VRETWT    HP+D TLYTGALGTAFLALKS+ VS NE DLKLCSEIVTAC+S+S  SR 
Sbjct: 61  VRETWTLSGRHPQDNTLYTGALGTAFLALKSFFVSKNENDLKLCSEIVTACESLSKTSRH 120

Query: 121 VSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLF 180
           V+FICGRAGVCALGAVAAK AN+GR V HYL KF+ IK P DLPNELLYGRAGFLWACLF
Sbjct: 121 VTFICGRAGVCALGAVAAKFANDGRLVDHYLAKFKDIKLPSDLPNELLYGRAGFLWACLF 180

Query: 181 LNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVL 240
           LNKHIG NTISN  MRSVVDEVIEAGR LG+K KSPLMYEWHGKKYWGAAHGLAGIMHVL
Sbjct: 181 LNKHIGQNTISNNFMRSVVDEVIEAGRTLGEKSKSPLMYEWHGKKYWGAAHGLAGIMHVL 240

Query: 241 MNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVE 300
           M+++L+PDE EDVK+T+RYMIK+RFPSGNY SSEG+ESDRLVHWCHGAPGVALTL KA E
Sbjct: 241 MDMELKPDEVEDVKNTLRYMIKNRFPSGNYPSSEGSESDRLVHWCHGAPGVALTLGKAAE 300

Query: 301 IFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFA 360
           +FGDD+FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YL+RAKAFA
Sbjct: 301 VFGDDEFLQAAIDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDLMYLHRAKAFA 360

Query: 361 CFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           CFLHQNAE+LISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEP+ A+FPSYEL
Sbjct: 361 CFLHQNAEKLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPNAAKFPSYEL 412

BLAST of MS021318 vs. NCBI nr
Match: XP_022940894.1 (lanC-like protein GCR2 [Cucurbita moschata])

HSP 1 Score: 693.7 bits (1789), Expect = 9.3e-196
Identity = 335/414 (80.92%), Postives = 373/414 (90.10%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDS----DALTLTELLSLPYAALSDHFRKAAFDLKE 60
           MADRFFP QMP FVAEAPA+ELA SDS    D LT ++LLSLP+AALS  F + A DLKE
Sbjct: 1   MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120
           TVVRETWT  E + +D+TLYTGALGTAFLALKSY+VSNNE DLKLCSEIV AC+++S DS
Sbjct: 61  TVVRETWTLSERYLQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 120

Query: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180
           RRVSF+CGRAGVCALGAVAAKLAN+GR V HYLEKF++IK P +LPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSNLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI  +TIS TIMRSVVDEVI+ GRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300
           VLMN++L+PDE EDVK+T+RYMIK+RFPSGN+ SSE N+SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360
            E+FGD +FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FACFLHQNA++LISEGKM SGDRP+S+FEGIGGMAYLFFDM EPS ARFP+YEL
Sbjct: 361 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of MS021318 vs. NCBI nr
Match: XP_038897162.1 (lanC-like protein GCR2 [Benincasa hispida])

HSP 1 Score: 693.3 bits (1788), Expect = 1.2e-195
Identity = 337/414 (81.40%), Postives = 371/414 (89.61%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDA----LTLTELLSLPYAALSDHFRKAAFDLKE 60
           M+DRFFPN+MP  +AE+PA++LAASDSD+    L  TELL LP AALSD  R+ A +LKE
Sbjct: 1   MSDRFFPNRMPRSLAESPADQLAASDSDSANHTLIPTELLPLPDAALSDRLRQTALNLKE 60

Query: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120
           TVVRETW     H +D+TLYTGALGTAFLALKSYLVS NE DLKLCSEIVTAC+SVS +S
Sbjct: 61  TVVRETWALGGRHVQDYTLYTGALGTAFLALKSYLVSKNENDLKLCSEIVTACESVSRNS 120

Query: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180
           RRVSFICG+AGVCALGAVAAKLAN+G  V HYLEKF+ IK P DLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFICGQAGVCALGAVAAKLANDGSLVDHYLEKFKDIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKH+G NTISNTIMRSVVDEVIEAGR LGKK KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHVGWNTISNTIMRSVVDEVIEAGRGLGKKRKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300
           VLMN++L+PDE EDVK+T+RYMIK+RFPSGNY SSEGN+SD LVHWCHGAPGVALTL+KA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNYPSSEGNKSDCLVHWCHGAPGVALTLVKA 300

Query: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360
            E+FGDD+FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  +LYRAKA
Sbjct: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDFIHLYRAKA 360

Query: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FACFLHQNA+ LISEGKMHSGD PYSLFEGIGGMAYLFFDM+EP  A+FPSYEL
Sbjct: 361 FACFLHQNAQTLISEGKMHSGDSPYSLFEGIGGMAYLFFDMIEPYAAKFPSYEL 414

BLAST of MS021318 vs. ExPASy Swiss-Prot
Match: F4IEM5 (LanC-like protein GCR2 OS=Arabidopsis thaliana OX=3702 GN=GCR2 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.0e-156
Identity = 265/410 (64.63%), Postives = 317/410 (77.32%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           M +RFF N+MP+FV E  + E         +LT+LLSLPY + S+   + A  +K+ VV 
Sbjct: 1   MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETW       RD+ LYTG LGTA+L  KSY V+ NE DLKLC E V ACD  S DS RV+
Sbjct: 61  ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           FICG AGVCALGAVAAK   + +    YL +F  I+ P DLP ELLYGRAG+LWACLFLN
Sbjct: 121 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           KHIG  +IS+  MRSVV+E+  AGRQLG KG  PLMYEWHGK+YWGAAHGLAGIM+VLM+
Sbjct: 181 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300
            +L+PDE +DVK T+ YMI++RFPSGNY SSEG++SDRLVHWCHGAPGVALTL+KA +++
Sbjct: 241 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 300

Query: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360
              +F+EAAM+A EV+W+RGLLKRVGICHGISGNTYVFLSLYRLT + KYLYRAKAFA F
Sbjct: 301 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 360

Query: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           L   +E+LISEG+MH GDRP+SLFEGIGGMAY+  DM +P+ A FP YEL
Sbjct: 361 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of MS021318 vs. ExPASy Swiss-Prot
Match: Q8VZQ6 (LanC-like protein GCL2 OS=Arabidopsis thaliana OX=3702 GN=GCL2 PE=2 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 5.6e-151
Identity = 257/411 (62.53%), Postives = 314/411 (76.40%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           MA RFF N MPDFV E   E ++  D    TL  LL++PY++LS   +++A DLKETVV 
Sbjct: 1   MAGRFFDNVMPDFVKE--KESVSGGD----TLRNLLAMPYSSLSQQLKRSALDLKETVVI 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETW        D+TLY+G LG AFL  ++Y V+ N  DL LC EIV ACD+ S  S  V+
Sbjct: 61  ETWGFSGQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASSGDVT 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           F+CGRAGVC LGAVAAKL+     +++YL +F  I+   DLPNELLYGR G+LWACLF+N
Sbjct: 121 FLCGRAGVCGLGAVAAKLSGEEDLLNYYLGQFRLIRLSSDLPNELLYGRVGYLWACLFIN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           K+IG  T+S+  +R V  E+I+ GR + KKG SPLM+EW+GK+YWGAAHGLAGIMHVLM+
Sbjct: 181 KYIGKETLSSDTIREVAQEIIKEGRSMAKKGSSPLMFEWYGKRYWGAAHGLAGIMHVLMD 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNY-CSSEGNESDRLVHWCHGAPGVALTLIKAVEI 300
           V+L+PDEAEDVK T++YMIK+RFPSGNY  S E  + D LVHWCHGAPG+ALTL KA E+
Sbjct: 241 VQLKPDEAEDVKGTLKYMIKNRFPSGNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEV 300

Query: 301 FGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFAC 360
           FG+ +FLEA+  AAEV+WNRGLLKRVGICHGISGN YVFL+LYR TG ++YLYRAKAFA 
Sbjct: 301 FGEREFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAKAFAS 360

Query: 361 FLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FL     +L+S+G+MH GD PYSLFEG+ GMAYLF DMV+PS ARFP YEL
Sbjct: 361 FLLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of MS021318 vs. ExPASy Swiss-Prot
Match: Q9FJN7 (LanC-like protein GCL1 OS=Arabidopsis thaliana OX=3702 GN=GCL1 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.4e-88
Identity = 173/397 (43.58%), Postives = 249/397 (62.72%), Query Frame = 0

Query: 36  LSLPYAALSDHFRKAAFDLKETVVRETW-------TSKESHPRDWTLYTGALGTAFLALK 95
           +SLP    ++ F +AA  LK  VV  TW        S      D T+YTG LGTAF  LK
Sbjct: 42  ISLP----TESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYTGLLGTAFTCLK 101

Query: 96  SYLVSNNETDLKLCSEIVTACDSVS-TDSRRVSFICGRAGVCALGAVAAKLANNGRQVSH 155
           SY V+ N  DL  C+EI+  C +V+   +R V+F+CGR GVC LGA+ A    +  +   
Sbjct: 102 SYEVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVANYRGDQSKRDF 161

Query: 156 YLEKFEYIKPPDDLP-----------NELLYGRAGFLWACLFLNKHIGHNTISNTIMRSV 215
           +L  F  +    +LP            +LLYGRAGFLWA LFLN+++G  T+ + ++  +
Sbjct: 162 FLGLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPI 221

Query: 216 VDEVIEAGRQLGKKGKS-PLMYEWHGKKYWGAAHGLAGIMHVLMNVKLQPDEAEDVKSTI 275
           V  ++  GR      ++ PL+Y +HG ++WGAA+GLAGI++VL++  L  ++ +DV+ T+
Sbjct: 222 VAAILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTL 281

Query: 276 RYMIKDRFP-SGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIFGDDK-FLEAAMDAA 335
           RYM+ +RFP SGNY  SEGN  D+LV W HGA G+A+TL KA ++F  ++ F EAA++A 
Sbjct: 282 RYMMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAG 341

Query: 336 EVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACFLHQNAERLISEGK 395
           EV+W  GL+K+VG+  G++GN Y FLSLYRLTGD  Y  RAKAFA +L ++A  L++   
Sbjct: 342 EVVWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTS 401

Query: 396 MHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
             + +  YSLF G+ G   L+FD+V P  ++FP YE+
Sbjct: 402 QET-EHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433

BLAST of MS021318 vs. ExPASy Swiss-Prot
Match: Q9JJK2 (LanC-like protein 2 OS=Mus musculus OX=10090 GN=Lancl2 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.7e-81
Identity = 178/435 (40.92%), Postives = 251/435 (57.70%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSD------ALTLTELLSLPY----AALSDHFRKA 60
           M +R FPN  PD+ A A A  LAA  ++       L  TE   LP+      + +  ++ 
Sbjct: 18  MEERSFPNPFPDYEAAASAAGLAAGSAEETGRVCPLPTTEDPGLPFHPNGKIVPNFIKRI 77

Query: 61  AFDLKETVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACD 120
              +K+ + +     K + P D + YTG  G A L L+ Y V+ ++T L    + V    
Sbjct: 78  QTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTGDQTYLLRSLDYVKR-T 137

Query: 121 SVSTDSRRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKF-----EYIKPPDDLPNEL 180
             +   RRV+F+CG AG  A+GAV      +  +    + K        +    +LP+EL
Sbjct: 138 LRNLSGRRVTFLCGDAGPLAVGAVIYHKLKSECESQECITKLLQMHRTIVCQESELPDEL 197

Query: 181 LYGRAGFLWACLFLNKHIGHNTISNTIMRSVVDEVIEAGRQLG----KKGKSPLMYEWHG 240
           LYGRAG+L+A L+LN  IG  T+  T ++ VV  +IE+G+ L     K  + PL+Y+WH 
Sbjct: 198 LYGRAGYLYALLYLNTEIGPGTVGETAIKEVVSAIIESGKSLSREERKSERCPLLYQWHR 257

Query: 241 KKYWGAAHGLAGIMHVLMNVKLQPDE---AEDVKSTIRYMIKDRFPSGNYCSSEGNESDR 300
           K+Y GAAHG+AGI ++LM  + + D+    E VK +I Y+   +F SGNY SS  NE+DR
Sbjct: 258 KQYVGAAHGMAGIYYMLMQPEAKVDQETLTEMVKPSIDYVRHKKFRSGNYPSSLSNETDR 317

Query: 301 LVHWCHGAPGVALTLIKAVEIFGDDKFLEAAMDAAEVIWNRGLLKR-VGICHGISGNTYV 360
           LVHWCHGAPGV   L++A ++F ++K+L+ AM+ ++VIW RGLL++  GICHG SGN Y 
Sbjct: 318 LVHWCHGAPGVIHVLLQAYQVFKEEKYLKEAMECSDVIWQRGLLRKGYGICHGTSGNGYS 377

Query: 361 FLSLYRLTGDTKYLYRAKAFACFLHQNAERLISEGK--MHSGDRPYSLFEGIGGMAYLFF 411
           FLSLYRLT D KYLYRA  F       AE  +  G       DRPYSLFEG+ G  +   
Sbjct: 378 FLSLYRLTQDKKYLYRACKF-------AEWCLDYGAHGCRIPDRPYSLFEGMAGAVHFLS 437

BLAST of MS021318 vs. ExPASy Swiss-Prot
Match: Q9NS86 (LanC-like protein 2 OS=Homo sapiens OX=9606 GN=LANCL2 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.1e-77
Identity = 175/436 (40.14%), Postives = 250/436 (57.34%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALT-------LTELLSLPY----AALSDHFRK 60
           M +R F N  PD+  EA A  L AS +   T        T+   LP+      + +  R+
Sbjct: 19  MEERAFVNPFPDY--EAAAGALLASGAAEETGCVRPPATTDEPGLPFHQDGKIIHNFIRR 78

Query: 61  AAFDLKETVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTAC 120
               +K+ + +     K + P D + YTG  G A L L+ Y V+ ++T L    + V   
Sbjct: 79  IQTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTCDQTYLLRSLDYVKR- 138

Query: 121 DSVSTDSRRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKF-----EYIKPPDDLPNE 180
              + + RRV+F+CG AG  A+GAV      +  +    + K        +    DLP+E
Sbjct: 139 TLRNLNGRRVTFLCGDAGPLAVGAVIYHKLRSDCESQECVTKLLQLQRSVVCQESDLPDE 198

Query: 181 LLYGRAGFLWACLFLNKHIGHNTISNTIMRSVVDEVIEAGRQLG----KKGKSPLMYEWH 240
           LLYGRAG+L+A L+LN  IG  T+  + ++ VV+ +IE+G+ L     K  + PL+Y+WH
Sbjct: 199 LLYGRAGYLYALLYLNTEIGPGTVCESAIKEVVNAIIESGKTLSREERKTERCPLLYQWH 258

Query: 241 GKKYWGAAHGLAGIMHVLMNVKLQPDE---AEDVKSTIRYMIKDRFPSGNYCSSEGNESD 300
            K+Y GAAHG+AGI ++LM    + D+    E VK +I Y+   +F SGNY SS  NE+D
Sbjct: 259 RKQYVGAAHGMAGIYYMLMQPAAKVDQETLTEMVKPSIDYVRHKKFRSGNYPSSLSNETD 318

Query: 301 RLVHWCHGAPGVALTLIKAVEIFGDDKFLEAAMDAAEVIWNRGLLKR-VGICHGISGNTY 360
           RLVHWCHGAPGV   L++A ++F ++K+L+ AM+ ++VIW RGLL++  GICHG +GN Y
Sbjct: 319 RLVHWCHGAPGVIHMLMQAYKVFKEEKYLKEAMECSDVIWQRGLLRKGYGICHGTAGNGY 378

Query: 361 VFLSLYRLTGDTKYLYRAKAFACFLHQNAERLISEGK--MHSGDRPYSLFEGIGGMAYLF 411
            FLSLYRLT D KYLYRA  F       AE  +  G       DRPYSLFEG+ G  +  
Sbjct: 379 SFLSLYRLTQDKKYLYRACKF-------AEWCLDYGAHGCRIPDRPYSLFEGMAGAIHFL 438

BLAST of MS021318 vs. ExPASy TrEMBL
Match: A0A6J1CCV8 (lanC-like protein GCR2 OS=Momordica charantia OX=3673 GN=LOC111010494 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.6e-241
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR
Sbjct: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS
Sbjct: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN
Sbjct: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN
Sbjct: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300
           VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF
Sbjct: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300

Query: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360
           GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF
Sbjct: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360

Query: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL
Sbjct: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 410

BLAST of MS021318 vs. ExPASy TrEMBL
Match: A0A6J1IZ37 (lanC-like protein GCR2 OS=Cucurbita maxima OX=3661 GN=LOC111480469 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.1e-197
Identity = 337/414 (81.40%), Postives = 374/414 (90.34%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDS----DALTLTELLSLPYAALSDHFRKAAFDLKE 60
           MADRFFPNQMP FVAEAPA+ELA SDS    D LT ++LLSLP+AALS  F + A DLKE
Sbjct: 1   MADRFFPNQMPHFVAEAPADELAPSDSDIAGDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120
           TVVR  WT  E +P+D+TLYTGALGTAFLALKSY+VSNNE DLKLCSEIV AC+++S DS
Sbjct: 61  TVVRGMWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETLSGDS 120

Query: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180
           RRVSF+CGRAGVCALGAVAAKLAN+GR V HYLEKF++IK P DLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI  +TIS TIMR+VVDEVI+AGRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRAVVDEVIKAGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300
           VLMN++L+PDE EDVK+T+RYMIK+RFPSGN+ SSEGN+SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFHSSEGNDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360
            E+FGD +FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FACFLHQNA+ LISEGKM SGDRP+S+FEGIGGMAYLFFDM EPS ARFP+YEL
Sbjct: 361 FACFLHQNAQMLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of MS021318 vs. ExPASy TrEMBL
Match: A0A0A0L279 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G638330 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 7.0e-197
Identity = 339/412 (82.28%), Postives = 369/412 (89.56%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTL--TELLSLPYAALSDHFRKAAFDLKETV 60
           MADRFFPNQMP F AEAP +ELA+S S + TL  TELLSLP AALSD  R+AA +LKETV
Sbjct: 1   MADRFFPNQMPRFPAEAPPDELASSHSYSATLIPTELLSLPDAALSDRLRQAALNLKETV 60

Query: 61  VRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRR 120
           VRETWT    HP+D TLYTGALGTAFLALKS+ VS NE DLKLCSEIVTAC+S+S  SR 
Sbjct: 61  VRETWTLSGRHPQDNTLYTGALGTAFLALKSFFVSKNENDLKLCSEIVTACESLSKTSRH 120

Query: 121 VSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLF 180
           V+FICGRAGVCALGAVAAK AN+GR V HYL KF+ IK P DLPNELLYGRAGFLWACLF
Sbjct: 121 VTFICGRAGVCALGAVAAKFANDGRLVDHYLAKFKDIKLPSDLPNELLYGRAGFLWACLF 180

Query: 181 LNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVL 240
           LNKHIG NTISN  MRSVVDEVIEAGR LG+K KSPLMYEWHGKKYWGAAHGLAGIMHVL
Sbjct: 181 LNKHIGQNTISNNFMRSVVDEVIEAGRTLGEKSKSPLMYEWHGKKYWGAAHGLAGIMHVL 240

Query: 241 MNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVE 300
           M+++L+PDE EDVK+T+RYMIK+RFPSGNY SSEG+ESDRLVHWCHGAPGVALTL KA E
Sbjct: 241 MDMELKPDEVEDVKNTLRYMIKNRFPSGNYPSSEGSESDRLVHWCHGAPGVALTLGKAAE 300

Query: 301 IFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFA 360
           +FGDD+FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YL+RAKAFA
Sbjct: 301 VFGDDEFLQAAIDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDLMYLHRAKAFA 360

Query: 361 CFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           CFLHQNAE+LISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEP+ A+FPSYEL
Sbjct: 361 CFLHQNAEKLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPNAAKFPSYEL 412

BLAST of MS021318 vs. ExPASy TrEMBL
Match: A0A6J1FLL9 (lanC-like protein GCR2 OS=Cucurbita moschata OX=3662 GN=LOC111446345 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 4.5e-196
Identity = 335/414 (80.92%), Postives = 373/414 (90.10%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDS----DALTLTELLSLPYAALSDHFRKAAFDLKE 60
           MADRFFP QMP FVAEAPA+ELA SDS    D LT ++LLSLP+AALS  F + A DLKE
Sbjct: 1   MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120
           TVVRETWT  E + +D+TLYTGALGTAFLALKSY+VSNNE DLKLCSEIV AC+++S DS
Sbjct: 61  TVVRETWTLSERYLQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 120

Query: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180
           RRVSF+CGRAGVCALGAVAAKLAN+GR V HYLEKF++IK P +LPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSNLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI  +TIS TIMRSVVDEVI+ GRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300
           VLMN++L+PDE EDVK+T+RYMIK+RFPSGN+ SSE N+SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360
            E+FGD +FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FACFLHQNA++LISEGKM SGDRP+S+FEGIGGMAYLFFDM EPS ARFP+YEL
Sbjct: 361 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of MS021318 vs. ExPASy TrEMBL
Match: A0A5A7V883 (LanC-like protein GCL2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003830 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.0e-191
Identity = 331/412 (80.34%), Postives = 363/412 (88.11%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTL--TELLSLPYAALSDHFRKAAFDLKETV 60
           MADRFFPN MP F AEAP++EL +S S + TL  TELLSLP AAL D  R+ A +LKETV
Sbjct: 1   MADRFFPNPMPRFPAEAPSDELVSSHSYSATLIPTELLSLPDAALFDRLRQTALNLKETV 60

Query: 61  VRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRR 120
           VRETWT    HP+D+TLYTGALGTAFLALKS+LV  NE DLKLCSEIV AC+S+S  SR 
Sbjct: 61  VRETWTLSGRHPQDYTLYTGALGTAFLALKSFLVFKNENDLKLCSEIVAACESLSKKSRH 120

Query: 121 VSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLF 180
           V+FICGRAGVCALGAVAAK AN+G  V HYLEKF+ IK P DLPNELLYGRAGFLWACLF
Sbjct: 121 VTFICGRAGVCALGAVAAKFANDGMLVDHYLEKFKDIKLPSDLPNELLYGRAGFLWACLF 180

Query: 181 LNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVL 240
           LNKHIG NTISNT MRSVVDEVIEAGR+LG+K KSPLMYEWHGKKYWGAAHGLAGIMHVL
Sbjct: 181 LNKHIGRNTISNTFMRSVVDEVIEAGRRLGQKSKSPLMYEWHGKKYWGAAHGLAGIMHVL 240

Query: 241 MNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVE 300
           M+++L+PDE EDVK+T+RYMIK+RF SGNY SSE +ESDRLVHWCHGAPGVALTL KA E
Sbjct: 241 MDMELKPDEVEDVKNTLRYMIKNRFLSGNYPSSEESESDRLVHWCHGAPGVALTLGKAAE 300

Query: 301 IFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFA 360
           +FGDD+FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YL+RAKAFA
Sbjct: 301 VFGDDEFLQAAIDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDLTYLHRAKAFA 360

Query: 361 CFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           CFLHQNAE+LISEGKMHSGD PYSLFEGIGGMAYLF DMVEP  A+FPSYEL
Sbjct: 361 CFLHQNAEKLISEGKMHSGDCPYSLFEGIGGMAYLFLDMVEPYAAKFPSYEL 412

BLAST of MS021318 vs. TAIR 10
Match: AT1G52920.1 (G protein coupled receptor )

HSP 1 Score: 553.5 bits (1425), Expect = 1.4e-157
Identity = 265/410 (64.63%), Postives = 317/410 (77.32%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           M +RFF N+MP+FV E  + E         +LT+LLSLPY + S+   + A  +K+ VV 
Sbjct: 1   MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETW       RD+ LYTG LGTA+L  KSY V+ NE DLKLC E V ACD  S DS RV+
Sbjct: 61  ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           FICG AGVCALGAVAAK   + +    YL +F  I+ P DLP ELLYGRAG+LWACLFLN
Sbjct: 121 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           KHIG  +IS+  MRSVV+E+  AGRQLG KG  PLMYEWHGK+YWGAAHGLAGIM+VLM+
Sbjct: 181 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIF 300
            +L+PDE +DVK T+ YMI++RFPSGNY SSEG++SDRLVHWCHGAPGVALTL+KA +++
Sbjct: 241 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 300

Query: 301 GDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACF 360
              +F+EAAM+A EV+W+RGLLKRVGICHGISGNTYVFLSLYRLT + KYLYRAKAFA F
Sbjct: 301 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 360

Query: 361 LHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           L   +E+LISEG+MH GDRP+SLFEGIGGMAY+  DM +P+ A FP YEL
Sbjct: 361 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of MS021318 vs. TAIR 10
Match: AT2G20770.1 (GCR2-like 2 )

HSP 1 Score: 535.4 bits (1378), Expect = 4.0e-152
Identity = 257/411 (62.53%), Postives = 314/411 (76.40%), Query Frame = 0

Query: 1   MADRFFPNQMPDFVAEAPAEELAASDSDALTLTELLSLPYAALSDHFRKAAFDLKETVVR 60
           MA RFF N MPDFV E   E ++  D    TL  LL++PY++LS   +++A DLKETVV 
Sbjct: 1   MAGRFFDNVMPDFVKE--KESVSGGD----TLRNLLAMPYSSLSQQLKRSALDLKETVVI 60

Query: 61  ETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDSRRVS 120
           ETW        D+TLY+G LG AFL  ++Y V+ N  DL LC EIV ACD+ S  S  V+
Sbjct: 61  ETWGFSGQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASSGDVT 120

Query: 121 FICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWACLFLN 180
           F+CGRAGVC LGAVAAKL+     +++YL +F  I+   DLPNELLYGR G+LWACLF+N
Sbjct: 121 FLCGRAGVCGLGAVAAKLSGEEDLLNYYLGQFRLIRLSSDLPNELLYGRVGYLWACLFIN 180

Query: 181 KHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMHVLMN 240
           K+IG  T+S+  +R V  E+I+ GR + KKG SPLM+EW+GK+YWGAAHGLAGIMHVLM+
Sbjct: 181 KYIGKETLSSDTIREVAQEIIKEGRSMAKKGSSPLMFEWYGKRYWGAAHGLAGIMHVLMD 240

Query: 241 VKLQPDEAEDVKSTIRYMIKDRFPSGNY-CSSEGNESDRLVHWCHGAPGVALTLIKAVEI 300
           V+L+PDEAEDVK T++YMIK+RFPSGNY  S E  + D LVHWCHGAPG+ALTL KA E+
Sbjct: 241 VQLKPDEAEDVKGTLKYMIKNRFPSGNYPASEEDKKKDILVHWCHGAPGIALTLGKAAEV 300

Query: 301 FGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFAC 360
           FG+ +FLEA+  AAEV+WNRGLLKRVGICHGISGN YVFL+LYR TG ++YLYRAKAFA 
Sbjct: 301 FGEREFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAKAFAS 360

Query: 361 FLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
           FL     +L+S+G+MH GD PYSLFEG+ GMAYLF DMV+PS ARFP YEL
Sbjct: 361 FLLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of MS021318 vs. TAIR 10
Match: AT5G65280.1 (GCR2-like 1 )

HSP 1 Score: 328.2 bits (840), Expect = 9.6e-90
Identity = 173/397 (43.58%), Postives = 249/397 (62.72%), Query Frame = 0

Query: 36  LSLPYAALSDHFRKAAFDLKETVVRETW-------TSKESHPRDWTLYTGALGTAFLALK 95
           +SLP    ++ F +AA  LK  VV  TW        S      D T+YTG LGTAF  LK
Sbjct: 42  ISLP----TESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYTGLLGTAFTCLK 101

Query: 96  SYLVSNNETDLKLCSEIVTACDSVS-TDSRRVSFICGRAGVCALGAVAAKLANNGRQVSH 155
           SY V+ N  DL  C+EI+  C +V+   +R V+F+CGR GVC LGA+ A    +  +   
Sbjct: 102 SYEVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVANYRGDQSKRDF 161

Query: 156 YLEKFEYIKPPDDLP-----------NELLYGRAGFLWACLFLNKHIGHNTISNTIMRSV 215
           +L  F  +    +LP            +LLYGRAGFLWA LFLN+++G  T+ + ++  +
Sbjct: 162 FLGLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQGTVPDHLLSPI 221

Query: 216 VDEVIEAGRQLGKKGKS-PLMYEWHGKKYWGAAHGLAGIMHVLMNVKLQPDEAEDVKSTI 275
           V  ++  GR      ++ PL+Y +HG ++WGAA+GLAGI++VL++  L  ++ +DV+ T+
Sbjct: 222 VAAILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLSEEDVKDVQGTL 281

Query: 276 RYMIKDRFP-SGNYCSSEGNESDRLVHWCHGAPGVALTLIKAVEIFGDDK-FLEAAMDAA 335
           RYM+ +RFP SGNY  SEGN  D+LV W HGA G+A+TL KA ++F  ++ F EAA++A 
Sbjct: 282 RYMMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKERDFREAAIEAG 341

Query: 336 EVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKAFACFLHQNAERLISEGK 395
           EV+W  GL+K+VG+  G++GN Y FLSLYRLTGD  Y  RAKAFA +L ++A  L++   
Sbjct: 342 EVVWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLCRDAIELVNMTS 401

Query: 396 MHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 411
             + +  YSLF G+ G   L+FD+V P  ++FP YE+
Sbjct: 402 QET-EHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433