MS021309 (gene) Bitter gourd (TR) v1

Overview


Name	MS021309
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	non-canonical poly(A) RNA polymerase PAPD5
Location	scaffold358: 922138 .. 926604 (+)
RNA-Seq Expression	MS021309
Synteny	MS021309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAGGAGACAATTCAGCAGTTTCTTTACGATACTCTAAGCCCACTTTCCTTAACCGCCGCCACTACCACTTCAAGCGAGGACCAACTGTCGCCGTCCGGCGATCTCGAGCCATACTCCGTTTTCCGGAACCAGATTTCTCTCTCTACTTCTCACTGTGCTCCGGTCGAGACCGCTGCGCCCGAGTTTTTCTCCCTCGATGTTGGTGCTGACAATGTGGAGGAAATTTCGGGTTTTTCTACTCCTTTGCCGGGTGCGTCGGTTCTGGAAACTGAGCCTAGGACGCCGGAACCTGAGGACCAGGCGAAGCTTGAAAGTGGGTGGTTCCGTGGCAATAGTAGATTCAAGAGCCCTATGCTACAGTTGCATAAAGGTAATTGAATCTGAGTTAACTTTTGTTTTGTTGCTCTTGAAGCAGTGTAGGCTGCAATATCTGTGCTTGGATGTAATTATGTTCAATCCTGATTGTTTTACGTGCAAGTTGAAGTTGTTTAGGATGATGTAGTGTTTAATTTCGAATGGTTACGCGCGTCGTTGAATTATCTCTTTGACGATAGAAGTTTTAATTTTCGATTCTCAAGTAAAAAAAGCATGTGGTGTGATAACAAACAGTAAGGCCCTTGATACCCTCATCCATATAATATTCACGTTGTATACATCATTGTAGTTCTGAAATTGTAACTTTGGTTGTATGCAGAAATTGTCGATTTTTGTGAATTTCTGTCTCCAACTGGAGAAGAACGCGATGAACGTGACAGGGCAGTAGAAAGGGTCTTTAGTGTTGTCAAACATATATGGCCTCACTGCAAGGTATGATTTGTGCCCCACATTATTTAGTGTTCCCCTATTCAATCCCAAGAGGATGGGGTGGTGTATTTATTTGTTCTTTTTGGTTTTCTAGGTTGAAGTATTTGGATCGTTCCAGACAGGACTCTATCTTCCAACCAGTGACATTGATGTGAGTTTCTAGATCCATATTTTATAATGTTTCATGATTTTGATCTCTTATTATAAGAGGTTATACTGCTTGCTGTATGCCTATTGGGATTAAAATGGACAAAACTACTAACTGAATGGTCAAACTGCAAATTGCATTTGACCCTTCAGAGCCAGCTGAATAACGAGATGGTGGATTTGGAAGCAATTACATTTTATTTTTTTCTTTTTTATGTCTCTTATCTTTTTTAGTTTTAAGTGCAACTATGCCTAGGGCATTGGCTAAAAAGTAAGAAATATTAGATACGTAGTACACCGAGTCAGGTGAAGTCAATGTGGTATAGGGTGATCCTCTATGAATGAGACATTCTGCTCTAGAATTTATGGTCTGCAATTTCATTTTAATTGAATAGTGGAAGCCTTTTTTATGTAACTGGATTGTTTCTATCTGCCCTCAGTTTTTCCTTTTCCTTGATTAGTGGTTTATGATAAAGCATTGGATATGGAAAATATTTGTTATTATATGGAACCAACTTTTATTTCTTACTGGAAACAAACTAATATTTATCTCTTCAGTTAATTATTGAATGTAACCATTTGCTGCAGGTTGTTATTCTGGGTTCAGGCATCCCTAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCCTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTATGCTTAATTTATGATTCACTCGTTATCGTCTCAGGATCTTCATTAACTATACTCTTGTATCTCCTATGATATCATTTGAACAGGTTATTGGAAAAGCCCGTGTGCCAATAATTAAGTTTGTCGAAAAGCAAAGTGGTATAGCTTTTGATATTAGGTATTTTGTCAATCTATTGGACTAGTATTGGAATTTATAAATTGGTTAAAAAAGTTTCTGCTTGCTATGAAGAAATCCCCCCTCTTCCTTGATAGAAAACAATTCGTTGATGAATTACAGACACATAGATTACGTAAAATTCAATAGTAGTCCAAAAAGTTGACATCTCATCTCTTGGTTAACAGTTTTGACGTGCAGAATGGACCAAAGGCTGCGGATTATATAAAGGTTTGTTGCTATTCTTCAAACTTGCTATGATGACCTTATCTCGATCATTGCAGGCTTGCAGTGTTTATGTTTTTGTGGTTTATATTATGAGGTGGCTGCTTGATGTGCAAATTATGCTTTAAGGTTTTCTCAAAATTTATCTCTTTCAAAAAATTGTAGTACCATTTCTGTGATATTTTGCAATTAGATATTATTGTGTATTATTGTCTTTTGCCTATTTCCTGCTTGGAGTTCAGCATGCACTTCTCTATCGTTTAATCTCATTATCACATGCTTGTTTTGAGTTATAGGTATACAAGGAATTTTTTTTGATTGATTATGGAATTTGAGTGCATATAATTGAATGGAAGTTTAGGATAGAATATTCAAATGAAAAGTGATGTTAACTTTCCATATTGTTTGATGGATTTGAATTATGTAGTTTCTTTATTTTTTCTTGAATTTGAGTGCATATAGTTGAATAGAAGCTTGGGTTGGAATACTCACATCAAATCTGAAGTTCACTTTTCTTAATTGTTTGATGGATTTGAATTATTTAGTTTATCTGCTGACTGCTAGTGCTTTTCCTCTTAACAGGTTGCAGTTGATAAGTGGCCTCCTTTGCGTCCTTTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATGGTTCCAACTACGAATTGTAATTCTTATCAAGTTGGTCCATCCATTACCTTTAAATTGTCTGTGTCTAATTCAAGTTTTCTGTAAGGTCTGCAGGTATATTCAGGTGGACTGGGTTCTTATGCACTTCTTACCATGCTTATGGCAATGTTGCAGGTTAGTATATTTTAATTTCAAATTCCACCTGTATATGACATTTTTCCTTCAAAAAATCATATATAACTTCCTAATTGTCCTTTCAGAAGGATGTTGAGACCTAGCCAAAATACTTCTGTTTGTGAAGCAATGTTTGGTTGTGCTGGAACTGAGAATAGAAAAAAGTAAATCAAACTTTTTTGTTTGGTGAACTATCTTTAGAATATATTCTTTTCATCTTAAGGATTACTTGGTATTTTGTCTAGTAAAGACAACGAACTACCGACATTCAATGATGTGTATTATCTTAAGGCATTTAGTCTCCCGCTGATTATTCTTTCATCTGGCCTCATTGATTGCAGAGTCACAACAGCTGCCAATCTTCTCTTGAACATAATCTTGGAGTCCTTTTGGTATGTTTTTTGTTTTTTGTTTCTTTTAACTTTTTAACTTTTTATTCAAAGCACCTACTAGCATTTTTAGTGAATGAAATTTCGAAGATGTGAAAATAGGTGCTTATAGATAATTTTTTCCTTAGAAATTAGCGTAGCTAATGCTGCTATTAGTATTTGTACTGTATTTTCTGTTAAACAGAAGTGGAGTAGAAGATTTCTTTGATTTATTATTAATGTCTTAATTTTCAATGATAGGTAAATTTTTTTGATTTCTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATGGAGGAGGCATTTTTTTCTCGAAGAGTAAAAGAGGGTATAAATATGAAAAATATTACAATGAATTTATATTCCATTCTCATTCTCAGACATCACATTTCATTACTTGCTATTTTCGCCTTTCGGCCTCTCCCTAAATTTTTGTTTTATTTGGTACTGTTGCAGATTCATGACAAAAGCACGTCCATTTCTTCTTTCCATTGAGGATCCTCAGGTTAATTTGCTAGTTTTAAAACAAAGTTTTGCAACTTGGTCACTGAGCTTTCCTTGCTTTTTATAAGTTATTGCTCTGATGCAATTTATTGATATTACCTTCCAGGCACCAGATAATGACATTGGCAAGAATTCCTTCAATTACTTCCAGGTTTGTTATCAATCATGTTGTTTATTGTGCCTGTCGTCCTTTTTATCTCAAGACTTTGTTTCTGATATCTACCCCGGTTGCAGATAAGATCTGCATTTGCACTGGCCTTTTCAACCTTGACAAATGTTAAGACCATTTTGGGTCTGGGTCCGAACAGGAGTATTCTTGGCACGATAATTAGGCCTGATCCAGTCCTGTTGAAGCGAAAAGGTGGCACAGATGGAGAAGTGACCTTCAATTCTCTACTGCCTGGAGCAGGGGAACCTATACAACTACAGTATGGAAATGAGCAAGAGATGCTGTGTAATTGGCAGTTTGGTGATGACGAAGAACCATTGCCGCGGGGGAATGATGCATCCGAAGATTTTGGTACGCCTTCCTCTAGGAAAAAGAGAAAAGCCAGTTCTCGCGAGAAGTCGAAAAAGAAGGAAAAAGAAAATAGTAGTAGTAAGCGAAGGCATGAAGAAAATGGATATAGTAAAGAGAAGAGTTCAAAGAAGAAACGATGGAGACAGAATGGCAGTGATGCCAATGGTTTTAGCCATTCTGGAAGAAGGTCCCCTTGGAAACAC

mRNA sequence

ATGGAGGAGGAGACAATTCAGCAGTTTCTTTACGATACTCTAAGCCCACTTTCCTTAACCGCCGCCACTACCACTTCAAGCGAGGACCAACTGTCGCCGTCCGGCGATCTCGAGCCATACTCCGTTTTCCGGAACCAGATTTCTCTCTCTACTTCTCACTGTGCTCCGGTCGAGACCGCTGCGCCCGAGTTTTTCTCCCTCGATGTTGGTGCTGACAATGTGGAGGAAATTTCGGGTTTTTCTACTCCTTTGCCGGGTGCGTCGGTTCTGGAAACTGAGCCTAGGACGCCGGAACCTGAGGACCAGGCGAAGCTTGAAAGTGGGTGGTTCCGTGGCAATAGTAGATTCAAGAGCCCTATGCTACAGTTGCATAAAGAAATTGTCGATTTTTGTGAATTTCTGTCTCCAACTGGAGAAGAACGCGATGAACGTGACAGGGCAGTAGAAAGGGTCTTTAGTGTTGTCAAACATATATGGCCTCACTGCAAGGTTGAAGTATTTGGATCGTTCCAGACAGGACTCTATCTTCCAACCAGTGACATTGATGTTGTTATTCTGGGTTCAGGCATCCCTAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCCTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTTATTGGAAAAGCCCGTGTGCCAATAATTAAGTTTGTCGAAAAGCAAAGTGGTATAGCTTTTGATATTAGTTTTGACGTGCAGAATGGACCAAAGGCTGCGGATTATATAAAGGTTGCAGTTGATAAGTGGCCTCCTTTGCGTCCTTTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATATTCAGGTGGACTGGGTTCTTATGCACTTCTTACCATGCTTATGGCAATGTTGCAGAGTCACAACAGCTGCCAATCTTCTCTTGAACATAATCTTGGAGTCCTTTTGGTAAATTTTTTTGATTTCTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATGGAGGAGGCATTTTTTTCTCGAAGAGTAAAAGAGGATTCATGACAAAAGCACGTCCATTTCTTCTTTCCATTGAGGATCCTCAGGCACCAGATAATGACATTGGCAAGAATTCCTTCAATTACTTCCAGATAAGATCTGCATTTGCACTGGCCTTTTCAACCTTGACAAATGTTAAGACCATTTTGGGTCTGGGTCCGAACAGGAGTATTCTTGGCACGATAATTAGGCCTGATCCAGTCCTGTTGAAGCGAAAAGGTGGCACAGATGGAGAAGTGACCTTCAATTCTCTACTGCCTGGAGCAGGGGAACCTATACAACTACAGTATGGAAATGAGCAAGAGATGCTGTGTAATTGGCAGTTTGGTGATGACGAAGAACCATTGCCGCGGGGGAATGATGCATCCGAAGATTTTGGTACGCCTTCCTCTAGGAAAAAGAGAAAAGCCAGTTCTCGCGAGAAGTCGAAAAAGAAGGAAAAAGAAAATAGTAGTAGTAAGCGAAGGCATGAAGAAAATGGATATAGTAAAGAGAAGAGTTCAAAGAAGAAACGATGGAGACAGAATGGCAGTGATGCCAATGGTTTTAGCCATTCTGGAAGAAGGTCCCCTTGGAAACAC

Coding sequence (CDS)

ATGGAGGAGGAGACAATTCAGCAGTTTCTTTACGATACTCTAAGCCCACTTTCCTTAACCGCCGCCACTACCACTTCAAGCGAGGACCAACTGTCGCCGTCCGGCGATCTCGAGCCATACTCCGTTTTCCGGAACCAGATTTCTCTCTCTACTTCTCACTGTGCTCCGGTCGAGACCGCTGCGCCCGAGTTTTTCTCCCTCGATGTTGGTGCTGACAATGTGGAGGAAATTTCGGGTTTTTCTACTCCTTTGCCGGGTGCGTCGGTTCTGGAAACTGAGCCTAGGACGCCGGAACCTGAGGACCAGGCGAAGCTTGAAAGTGGGTGGTTCCGTGGCAATAGTAGATTCAAGAGCCCTATGCTACAGTTGCATAAAGAAATTGTCGATTTTTGTGAATTTCTGTCTCCAACTGGAGAAGAACGCGATGAACGTGACAGGGCAGTAGAAAGGGTCTTTAGTGTTGTCAAACATATATGGCCTCACTGCAAGGTTGAAGTATTTGGATCGTTCCAGACAGGACTCTATCTTCCAACCAGTGACATTGATGTTGTTATTCTGGGTTCAGGCATCCCTAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCCTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTTATTGGAAAAGCCCGTGTGCCAATAATTAAGTTTGTCGAAAAGCAAAGTGGTATAGCTTTTGATATTAGTTTTGACGTGCAGAATGGACCAAAGGCTGCGGATTATATAAAGGTTGCAGTTGATAAGTGGCCTCCTTTGCGTCCTTTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATATTCAGGTGGACTGGGTTCTTATGCACTTCTTACCATGCTTATGGCAATGTTGCAGAGTCACAACAGCTGCCAATCTTCTCTTGAACATAATCTTGGAGTCCTTTTGGTAAATTTTTTTGATTTCTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATGGAGGAGGCATTTTTTTCTCGAAGAGTAAAAGAGGATTCATGACAAAAGCACGTCCATTTCTTCTTTCCATTGAGGATCCTCAGGCACCAGATAATGACATTGGCAAGAATTCCTTCAATTACTTCCAGATAAGATCTGCATTTGCACTGGCCTTTTCAACCTTGACAAATGTTAAGACCATTTTGGGTCTGGGTCCGAACAGGAGTATTCTTGGCACGATAATTAGGCCTGATCCAGTCCTGTTGAAGCGAAAAGGTGGCACAGATGGAGAAGTGACCTTCAATTCTCTACTGCCTGGAGCAGGGGAACCTATACAACTACAGTATGGAAATGAGCAAGAGATGCTGTGTAATTGGCAGTTTGGTGATGACGAAGAACCATTGCCGCGGGGGAATGATGCATCCGAAGATTTTGGTACGCCTTCCTCTAGGAAAAAGAGAAAAGCCAGTTCTCGCGAGAAGTCGAAAAAGAAGGAAAAAGAAAATAGTAGTAGTAAGCGAAGGCATGAAGAAAATGGATATAGTAAAGAGAAGAGTTCAAAGAAGAAACGATGGAGACAGAATGGCAGTGATGCCAATGGTTTTAGCCATTCTGGAAGAAGGTCCCCTTGGAAACAC

Protein sequence

MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETAAPEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSHNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLSIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSPWKH

Homology

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BLAST of MS021309 vs. NCBI nr
Match: XP_022139704.1 (non-canonical poly(A) RNA polymerase PAPD5 [Momordica charantia])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.5e-307
Identity = 539/543 (99.26%), Postives = 541/543 (99.63%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA
Sbjct: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM
Sbjct: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTGEERDERD+AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTGEERDERDKAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD
Sbjct: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
           HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS
Sbjct: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS 480
           RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS
Sbjct: 421 RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS 480

Query: 481 RKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP 540
           RKKRK S REKSKKKE+ENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP
Sbjct: 481 RKKRKVSCREKSKKKERENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP 540

Query: 541 WKH 544
           WKH
Sbjct: 541 WKH 543

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BLAST of MS021309 vs. NCBI nr
Match: XP_038899128.1 (terminal nucleotidyltransferase 4B [Benincasa hispida])

HSP 1 Score: 916.8 bits (2368), Expect = 9.0e-263
Identity = 472/541 (87.25%), Postives = 496/541 (91.68%), Query Frame = 0


Query: 2   EEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETAA 61
           EEE +Q +LYDTLSPLS +A TTT S DQ SPS DLEPYSVFRN+ISLST+H AP ETAA
Sbjct: 3   EEEVVQLYLYDTLSPLSFSAVTTTESGDQFSPSDDLEPYSVFRNEISLSTTHHAPAETAA 62

Query: 62  PEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPML 121
           PEFF+LDV ADNVEEISG STPLPG S LETEPRTPE EDQ+KLESGWFRGNS FKSPML
Sbjct: 63  PEFFALDVAADNVEEISGLSTPLPGTSALETEPRTPEREDQSKLESGWFRGNSGFKSPML 122

Query: 122 QLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDI 181
           QLHKEIVDF EFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDI
Sbjct: 123 QLHKEIVDFSEFLSPTEEERVARDNAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDI 182

Query: 182 DVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFDV 241
           DVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEK SGI+FDISFDV
Sbjct: 183 DVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKLSGISFDISFDV 242

Query: 242 QNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSH 301
           QNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 
Sbjct: 243 QNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSF 302

Query: 302 NSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLSI 361
           N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RP LLSI
Sbjct: 303 NGPHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNAGGIFFSKSYRGFMTKGRPCLLSI 362

Query: 362 EDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLKR 421
           EDPQAPDNDIGKNSFNYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLKR
Sbjct: 363 EDPQAPDNDIGKNSFNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLKR 422

Query: 422 KGGTDGEVTFNSLLPGAGEPIQL-QYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS 481
           KGG DGEVTFNSLLPGAGEPIQ  +YG++ EMLCNWQFGDD EPLPRGNDAS++FGTPSS
Sbjct: 423 KGGRDGEVTFNSLLPGAGEPIQQPEYGDDLEMLCNWQFGDD-EPLPRGNDASQNFGTPSS 482

Query: 482 RKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP 541
           +K+RK  SR KS+KK+KEN  SKRRHE+NGYSKEKSSKKKRW+ NGSDANGF H+GRRSP
Sbjct: 483 KKRRK--SRNKSRKKQKENHKSKRRHEDNGYSKEKSSKKKRWKLNGSDANGFGHAGRRSP 540

[Close]

BLAST of MS021309 vs. NCBI nr
Match: XP_022936827.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata] >XP_022936828.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata])

HSP 1 Score: 872.1 bits (2252), Expect = 2.5e-249
Identity = 459/544 (84.38%), Postives = 482/544 (88.60%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEET+ Q+LYDTLSPLS +A  TT SE+QLSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FD+SFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDMSFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASGNLDTP 480

Query: 481 SSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540
           SS+KKRK  SREKS+ KEKEN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSKKKRK--SREKSRNKEKENRGSSKR------SKEKSSRKKRWRENGSD-------GRR 528

Query: 541 SPWK 543
           SPWK
Sbjct: 541 SPWK 528

[Close]

BLAST of MS021309 vs. NCBI nr
Match: XP_023535086.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo] >XP_023535087.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871.7 bits (2251), Expect = 3.3e-249
Identity = 459/544 (84.38%), Postives = 482/544 (88.60%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEET+ Q+LYDTLSPLS +A  TT SE++LSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEKLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASGNLDTP 480

Query: 481 SSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540
           SS+KKRK  SREKS+ KEKEN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSKKKRK--SREKSRNKEKENRGSSKR------SKEKSSRKKRWRENGSD-------GRR 528

Query: 541 SPWK 543
           SPWK
Sbjct: 541 SPWK 528

[Close]

BLAST of MS021309 vs. NCBI nr
Match: XP_022976478.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima] >XP_022976479.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima])

HSP 1 Score: 871.7 bits (2251), Expect = 3.3e-249
Identity = 459/544 (84.38%), Postives = 482/544 (88.60%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEET+QQ+LYDTLSPLS +A  TT SE+QLSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASRNLDTP 480

Query: 481 SSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540
           SS+KKRK S  EKS+ K+KEN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSKKKRK-SRCEKSRNKDKENHGSSKR------SKEKSSRKKRWRENGSD-------GRR 529

Query: 541 SPWK 543
           SPWK
Sbjct: 541 SPWK 529

[Close]

BLAST of MS021309 vs. ExPASy Swiss-Prot
Match: Q68ED3 (Terminal nucleotidyltransferase 4B OS=Mus musculus OX=10090 GN=Tent4b PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 3.0e-59
Identity = 158/406 (38.92%), Postives = 226/406 (55.67%), Query Frame = 0


Query: 109 WFRGNSRFKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFG 168
           W R N  +   ++ LH+EI DF E++SP  EE   R   V R+ SV+K +WP   V++FG
Sbjct: 122 WKRRN--YNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVSRIESVIKELWPSADVQIFG 181

Query: 169 SFQTGLYLPTSDIDVVILGSGIPKPQLGLQALSRALSQKGIAKK--IQVIGKARVPIIKF 228
           SF+TGLYLPTSDID+V+ G       L L  L  AL +  +A +  ++V+ KA VPIIK 
Sbjct: 182 SFKTGLYLPTSDIDLVVFGKW---ENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKL 241

Query: 229 VEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            +  + +  DISF+VQNG +AAD IK    K+P L  L L+LK FL QR+LNEV++GG+G
Sbjct: 242 TDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIG 301

Query: 289 SYALLTMLMAMLQSHNSCQSSLEH-NLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSK 348
           SY+L  M ++ LQ H    + + + N GVLL+ FF+ YGR  N    G+    GG + +K
Sbjct: 302 SYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAK 361

Query: 349 S--KRGFMTKARPFLLSIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTN-VKTILGLG 408
              ++  +   RP +L IEDP  P ND+G++S+   Q++ AF  A+  L++ V  I    
Sbjct: 362 DEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYY 421

Query: 409 PN---RSILGTIIR-PDPVLLKRKGGTDGEVTFNSLLPGA-GEPIQLQYGNEQEMLCNWQ 468
           PN    SILG IIR  D V   R   +      N   P   G  + L    +Q   CN  
Sbjct: 422 PNNETESILGRIIRVTDEVATYRDWISKQWGLQNRPEPSCNGNGVTLIVDTQQLDKCNNN 481

Query: 469 FGDDEEPLPR-GNDASEDFGTPSSRKKRKASSREKSKKKEKENSSS 503
             +++E L +  ++ASE    P S+    +SS   S     ++SSS
Sbjct: 482 LSEEKEALGKCRSNASE----PLSKHSSNSSSGPVSSSSATQSSSS 518

[Close]

BLAST of MS021309 vs. ExPASy Swiss-Prot
Match: Q7KVS9 (Non-canonical poly(A) RNA polymerase protein Trf4-1 OS=Drosophila melanogaster OX=7227 GN=Trf4-1 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.1e-58
Identity = 129/312 (41.35%), Postives = 192/312 (61.54%), Query Frame = 0


Query: 109 WFRGNSRFKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFG 168
           W + +  +   ++ LH+EI  F +++ PT  E   R+  V+R+ +VV  IWP   VE+FG
Sbjct: 257 WRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFG 316

Query: 169 SFQTGLYLPTSDIDVVILGSGIPKPQLGLQALSRALSQKGIAK--KIQVIGKARVPIIKF 228
           SF+TGL+LPTSDID+V+LG      +L L+ L   L  +GIA+   ++V+ KA VPIIK 
Sbjct: 317 SFRTGLFLPTSDIDLVVLGLW---EKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKL 376

Query: 229 VEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            ++++ +  DISF++Q+G ++A+ IK     +P L  L L+LK FL  R+LNEV++GG+ 
Sbjct: 377 TDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGIS 436

Query: 289 SYALLTMLMAMLQSHNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKS 348
           SY+L+ M ++ LQ H         NLGVLL+ FF+ YGR+ N   +G+S   GG +  K 
Sbjct: 437 SYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKD 496

Query: 349 --KRGFMTKARPFLLSIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTL---TNVKTILGL 408
             +R  +   RP LL IEDP  P NDIG++S+  FQ++ AF  A+  L    +   +LG+
Sbjct: 497 ELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGI 556

Query: 409 GPN-RSILGTII 413
            P   SILG II
Sbjct: 557 DPRVNSILGRII 565

[Close]

BLAST of MS021309 vs. ExPASy Swiss-Prot
Match: Q5XG87 (Terminal nucleotidyltransferase 4A OS=Homo sapiens OX=9606 GN=TENT4A PE=1 SV=3)

HSP 1 Score: 228.4 bits (581), Expect = 1.9e-58
Identity = 147/396 (37.12%), Postives = 223/396 (56.31%), Query Frame = 0


Query: 114 SRFKSPMLQ-LHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQT 173
           SR  SP +Q LH+EI+DF  F+SP  EE   R   V+R+ +VVK +WP   V++FGSF T
Sbjct: 210 SRAYSPGIQGLHEEIIDFYNFMSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFST 269

Query: 174 GLYLPTSDIDVVILGSGIPKPQLGLQALSRALSQKGIAK--KIQVIGKARVPIIKFVEKQ 233
           GLYLPTSDID+V+ G     P   LQ L +AL +  +A+   I+V+ KA VPIIK  +++
Sbjct: 270 GLYLPTSDIDLVVFGKWERPP---LQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQE 329

Query: 234 SGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYAL 293
           + +  DISF+++ G +AA++IK  + K+  L  L L+LK FL QR+LNEV++GG+ SY+L
Sbjct: 330 TEVKVDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQRDLNEVFTGGISSYSL 389

Query: 294 LTMLMAMLQSHNSCQS-SLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSK-- 353
           + M ++ LQ H    +   + NLG+LLV FF+ YGR  N    G+    GG + +K +  
Sbjct: 390 ILMAISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYIAKEEIM 449

Query: 354 RGFMTKARPFLLSIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLG-PNR- 413
           +   +  RP +L IEDP  P ND+G++S+   Q++  F  A+  L++  + L    PNR 
Sbjct: 450 KAMTSGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRD 509

Query: 414 --SILGTIIRPDPVL------LKRKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQ 473
             S LG II+    +      +K K G+    +     PG    I+++   E+   CN +
Sbjct: 510 AESTLGRIIKVTQEVIDYRRWIKEKWGSKAHPS-----PGMDSRIKIK---ERIATCNGE 569

Query: 474 FGDDEEP-LPRGNDASEDFGTPSSRKKRKASSREKS 493
              + EP  P G   +    +P       ++S   S
Sbjct: 570 QTQNREPESPYGQRLTLSLSSPQLLSSGSSASSVSS 594

[Close]

BLAST of MS021309 vs. ExPASy Swiss-Prot
Match: Q8NDF8 (Terminal nucleotidyltransferase 4B OS=Homo sapiens OX=9606 GN=TENT4B PE=1 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 4.9e-54
Identity = 160/454 (35.24%), Postives = 233/454 (51.32%), Query Frame = 0


Query: 109 WFRGNSRFKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFG 168
           W R N  +   ++ LH+EI DF E++SP  EE   R   V R+ SV+K +WP   V++FG
Sbjct: 108 WKRRN--YNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFG 167

Query: 169 SFQTGLYLPTSDIDVVILGSGIPKPQLGLQALSRALSQKGIAKK--IQVIGKARVPIIKF 228
           SF+TGLYLPTSDID+V+ G       L L  L  AL +  +A +  ++V+ KA VPIIK 
Sbjct: 168 SFKTGLYLPTSDIDLVVFGKW---ENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKL 227

Query: 229 VEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            +  + +  DISF+VQNG +AAD IK    K+P L  L L+LK FL QR+LNEV++GG+G
Sbjct: 228 TDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIG 287

Query: 289 SYALLTMLMAMLQSHNSCQSSLEH-NLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSK 348
           SY+L  M ++ LQ H    + + + N GVLL+ FF+ YGR  N    G+    GG + +K
Sbjct: 288 SYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAK 347

Query: 349 S--KRGFMTKARPFLLSIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTN-VKTILGLG 408
              ++  +   RP +L IEDP  P ND+G++S+   Q++ AF  A+  L++ V  I    
Sbjct: 348 DEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYY 407

Query: 409 PN---RSILGTIIRPDPVLLKRKGGTDGEVTFNSLLPGAGEPIQLQYG--NEQEMLCNWQ 468
           PN    SILG IIR           TD   T+        + I  Q+G  N  E  CN  
Sbjct: 408 PNNETESILGRIIRV----------TDEVATYR-------DWISKQWGLKNRPEPSCNGP 467

Query: 469 FGDDEEPLPRGNDASEDFGTPSSRKKR-----KASSREKSKKKEKENS-------SSKRR 528
                      +D   D  TP    K+        +R  S+    E+S       S++  
Sbjct: 468 VSSSSATQSSSSDVDSD-ATPCKTPKQLLCRPSTGNRVGSQDVSLESSQAVGKMQSTQTT 527

Query: 529 HEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRS 540
           +  N  +K +    + +R +     G + +   S
Sbjct: 528 NTSNSTNKSQHGSARLFRSSSKGFQGTTQTSHGS 538

[Close]

BLAST of MS021309 vs. ExPASy Swiss-Prot
Match: Q6PB75 (Terminal nucleotidyltransferase 4A OS=Mus musculus OX=10090 GN=Tent4a PE=2 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 1.4e-53
Identity = 119/289 (41.18%), Postives = 178/289 (61.59%), Query Frame = 0


Query: 134 LSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILGSGIPKP 193
           +SP  EE   R   V+R+ +VVK +WP   V++FGSF TGLYLPTSDID+V+ G     P
Sbjct: 1   MSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPP 60

Query: 194 QLGLQALSRALSQKGIAK--KIQVIGKARVPIIKFVEKQSGIAFDISFDVQNGPKAADYI 253
              LQ L +AL +  +A+   I+V+ KA VPIIK  ++++ +  DISF+++ G +AA++I
Sbjct: 61  ---LQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFI 120

Query: 254 KVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSHNSCQS-SLEH 313
           K  + K+  L  L L+LK FL QR+LNEV++GG+ SY+L+ M ++ LQ H    +   + 
Sbjct: 121 KNYMKKYSLLPYLILVLKQFLLQRDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADE 180

Query: 314 NLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSK--RGFMTKARPFLLSIEDPQAPD 373
           NLG+LLV FF+ YGR  N    G+    GG + +K +  +   +  RP +L IEDP  P 
Sbjct: 181 NLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYIAKEEIMKAMTSGYRPSMLCIEDPLLPG 240

Query: 374 NDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLG-PNR---SILGTIIR 414
           ND+G++S+   Q++  F  A+  L++  + L    PNR   S LG II+
Sbjct: 241 NDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDSESTLGRIIK 286

[Close]

BLAST of MS021309 vs. ExPASy TrEMBL
Match: A0A6J1CDS2 (non-canonical poly(A) RNA polymerase PAPD5 OS=Momordica charantia OX=3673 GN=LOC111010551 PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.7e-307
Identity = 539/543 (99.26%), Postives = 541/543 (99.63%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA
Sbjct: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM
Sbjct: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTGEERDERD+AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTGEERDERDKAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD
Sbjct: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
           HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS
Sbjct: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS 480
           RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS
Sbjct: 421 RKGGTDGEVTFNSLLPGAGEPIQLQYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPSS 480

Query: 481 RKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP 540
           RKKRK S REKSKKKE+ENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP
Sbjct: 481 RKKRKVSCREKSKKKERENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRSP 540

Query: 541 WKH 544
           WKH
Sbjct: 541 WKH 543

[Close]

BLAST of MS021309 vs. ExPASy TrEMBL
Match: A0A6J1F9G2 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita moschata OX=3662 GN=LOC111443297 PE=4 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.2e-249
Identity = 459/544 (84.38%), Postives = 482/544 (88.60%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEET+ Q+LYDTLSPLS +A  TT SE+QLSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FD+SFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDMSFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASGNLDTP 480

Query: 481 SSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540
           SS+KKRK  SREKS+ KEKEN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSKKKRK--SREKSRNKEKENRGSSKR------SKEKSSRKKRWRENGSD-------GRR 528

Query: 541 SPWK 543
           SPWK
Sbjct: 541 SPWK 528

[Close]

BLAST of MS021309 vs. ExPASy TrEMBL
Match: A0A6J1IMA1 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita maxima OX=3661 GN=LOC111476864 PE=4 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.6e-249
Identity = 459/544 (84.38%), Postives = 482/544 (88.60%), Query Frame = 0


Query: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60
           MEEET+QQ+LYDTLSPLS +A  TT SE+QLSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASRNLDTP 480

Query: 481 SSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540
           SS+KKRK S  EKS+ K+KEN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSKKKRK-SRCEKSRNKDKENHGSSKR------SKEKSSRKKRWRENGSD-------GRR 529

Query: 541 SPWK 543
           SPWK
Sbjct: 541 SPWK 529

[Close]

BLAST of MS021309 vs. ExPASy TrEMBL
Match: A0A0A0L271 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637740 PE=4 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 7.5e-247
Identity = 454/542 (83.76%), Postives = 485/542 (89.48%), Query Frame = 0


Query: 3   EETIQQFLYDTLSPLSLTAATTTSSEDQL-SPSGDLEPYSVFRNQISLSTSHCAPVETAA 62
           EE +Q +LYDTLSPLS +A TTT++ DQL SP  DLEPYSVFRN+ISLST  CAP ETAA
Sbjct: 4   EEAVQHYLYDTLSPLSFSAITTTTTGDQLSSPDVDLEPYSVFRNEISLSTPDCAPAETAA 63

Query: 63  PEFFSLDVGADNVEEISGF-STPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 122
            EFF+LDV AD  EE SG  S+PLP  S LETEPRTPE EDQ++LESGWFRGNS  KSPM
Sbjct: 64  TEFFALDVAADKGEENSGICSSPLPVTSALETEPRTPECEDQSRLESGWFRGNSGLKSPM 123

Query: 123 LQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 182
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 124 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 183

Query: 183 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 242
           IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKF+EKQSGI+FDISFD
Sbjct: 184 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFIEKQSGISFDISFD 243

Query: 243 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 302
           VQNGPKAAD+IK AV KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 244 VQNGPKAADFIKGAVSKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 303

Query: 303 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 362
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RP LLS
Sbjct: 304 INVPPSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNAGGIFFSKSYRGFMTKGRPCLLS 363

Query: 363 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 422
           IEDPQAPDNDIGKNSFNYFQIRSAFA+A+S LTNVKT+LGLGPNRSILGTIIRPDPVLLK
Sbjct: 364 IEDPQAPDNDIGKNSFNYFQIRSAFAMAYSILTNVKTVLGLGPNRSILGTIIRPDPVLLK 423

Query: 423 RKGGTDGEVTFNSLLPGAGEPIQL-QYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTPS 482
           RKGG  GEVTFNSLLPGAGEP+Q  +YG++QEMLCNWQFG DEEPLPRGND  E+ GTPS
Sbjct: 424 RKGGRHGEVTFNSLLPGAGEPVQQPEYGDDQEMLCNWQFG-DEEPLPRGNDTPENVGTPS 483

Query: 483 SRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRRS 542
           S+K+RK  +REKS+KKEKE+ SSKRRHE+NG  KE+SSKKKR RQN SDANG  ++GRRS
Sbjct: 484 SKKQRK--TREKSRKKEKESHSSKRRHEDNGSRKEQSSKKKRLRQNDSDANGLWNAGRRS 542

[Close]

BLAST of MS021309 vs. ExPASy TrEMBL
Match: A0A1S3BTA1 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucumis melo OX=3656 GN=LOC103493489 PE=4 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.3e-246
Identity = 453/544 (83.27%), Postives = 486/544 (89.34%), Query Frame = 0


Query: 3   EETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETAAP 62
           EE +QQ+LYDTLSPLS +  +TT++ DQLS   DLEPYSVFRN+ISLST HCAP E AA 
Sbjct: 4   EEAVQQYLYDTLSPLSFSPISTTAAGDQLSSPDDLEPYSVFRNEISLSTPHCAPAEAAAT 63

Query: 63  EFFSLDVGADNVEEIS-GFSTPLPGASVLETEPRTP--EPEDQAKLESGWFRGNSRFKSP 122
           EFF+LDV +DNVEE S GFSTPL   S LETEPRTP  E EDQ++LESGWFRGNS FKSP
Sbjct: 64  EFFALDVASDNVEENSGGFSTPLLVTSALETEPRTPECEGEDQSRLESGWFRGNSGFKSP 123

Query: 123 MLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS 182
           MLQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS
Sbjct: 124 MLQLHKEIVDFCEFLSPTEEERFARDNAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS 183

Query: 183 DIDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISF 242
           DIDVVILGSGIPKPQLGLQALSRALSQ+G+AKKIQVIGKARVPIIKF+EKQSGI+FDISF
Sbjct: 184 DIDVVILGSGIPKPQLGLQALSRALSQRGMAKKIQVIGKARVPIIKFIEKQSGISFDISF 243

Query: 243 DVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ 302
           DVQNGPKAAD+IK A+ KWPPLRPL LILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ
Sbjct: 244 DVQNGPKAADFIKGAISKWPPLRPLSLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ 303

Query: 303 SHNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLL 362
           S N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RP LL
Sbjct: 304 SINVQHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNAGGIFFSKSYRGFMTKGRPCLL 363

Query: 363 SIEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLL 422
           SIEDPQAPDNDIGKNSFNYFQIRSAFA+A+S LTNVKTILGLGPNRSILGTIIRPDPVLL
Sbjct: 364 SIEDPQAPDNDIGKNSFNYFQIRSAFAMAYSILTNVKTILGLGPNRSILGTIIRPDPVLL 423

Query: 423 KRKGGTDGEVTFNSLLPGAGEPIQL--QYGNEQEMLCNWQFGDDEEPLPRGNDASEDFGT 482
           KRKGG  GEVTFNSLLPGAGEP+Q   Q G++QEMLCNWQFGDD EPLPRGN+  E+ GT
Sbjct: 424 KRKGGRHGEVTFNSLLPGAGEPLQQPDQCGDDQEMLCNWQFGDD-EPLPRGNNTPENVGT 483

Query: 483 PSSRKKRKASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGR 542
           PSS+KKRK  +REKS+KKEKE+  SKRRHE+NG  KEKSSKKKR RQNG+DANG  ++GR
Sbjct: 484 PSSKKKRK--TREKSRKKEKESHMSKRRHEDNGSRKEKSSKKKRSRQNGNDANGLWNAGR 543

[Close]

BLAST of MS021309 vs. TAIR 10
Match: AT5G53770.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 581.6 bits (1498), Expect = 6.4e-166
Identity = 320/517 (61.90%), Postives = 384/517 (74.27%), Query Frame = 0


Query: 9   FLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETAAPEFFSLD 68
           F+YDTL PLS     + S++       +   YSVFR +IS       PVE+A  +FFSLD
Sbjct: 13  FVYDTLPPLSF----SDSNQSPPPTHEESHQYSVFRKEISDFPDDTTPVESATVDFFSLD 72

Query: 69  VGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPMLQLHKEIV 128
           V  +  E      TP+  AS  +++ R  + +++ +LES WF  NS  K PMLQLHKEIV
Sbjct: 73  VEGETTENGVEPVTPVVVASKKKSKKR--KKDEEPRLESNWFSENSFSKIPMLQLHKEIV 132

Query: 129 DFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILGS 188
           DFC+FL PT  E+ ERD AVE V SV+K+IWP CKVEVFGS++TGLYLPTSDIDVVIL S
Sbjct: 133 DFCDFLLPTQAEKAERDAAVESVSSVIKYIWPSCKVEVFGSYKTGLYLPTSDIDVVILES 192

Query: 189 GIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFDVQNGPKAA 248
           G+  PQLGL+ALSRALSQ+GIAK + VI KARVPIIKFVEK+S IAFD+SFD++NGPKAA
Sbjct: 193 GLTNPQLGLRALSRALSQRGIAKNLLVIAKARVPIIKFVEKKSNIAFDLSFDMENGPKAA 252

Query: 249 DYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSHNSCQSSL 308
           ++I+ AV K PPLRPLCLILKVFLQQRELNEVYSGG+GSYALL ML+A L+     +S+ 
Sbjct: 253 EFIQDAVSKLPPLRPLCLILKVFLQQRELNEVYSGGIGSYALLAMLIAFLKYLKDGRSAP 312

Query: 309 EHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLSIEDPQAPD 368
           EHNLGVLLV FFDFYGRKLNT+DVG+SC  GG FFSK  +GF+ +ARP L+SIEDPQ P+
Sbjct: 313 EHNLGVLLVKFFDFYGRKLNTADVGISCKMGGSFFSKYNKGFLNRARPSLISIEDPQTPE 372

Query: 369 NDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGTDGE 428
           NDIGK+SFNYFQIRSAFA+A STLTN K IL LGPNRSILGTIIRPD VL +RKGG +G+
Sbjct: 373 NDIGKSSFNYFQIRSAFAMALSTLTNTKAILSLGPNRSILGTIIRPDRVLSERKGGQNGD 432

Query: 429 VTFNSLLPGAGEPIQLQYGNEQE--MLCNWQFGDDEE--PLPRGNDASEDFGTPSSRKKR 488
           VTFNSLLPGAGEP+ L+   +    + CNW+  ++EE    PRGND +    TP   KK 
Sbjct: 433 VTFNSLLPGAGEPLPLESNGKTNGGLFCNWELEEEEEEGSFPRGNDITPVVDTPG--KKS 492

Query: 489 KASSREKSKKKEKENSSSKRRHEENGYSKEKSSKKKR 522
           K SSR+K KKK K+N       EE G S +K  KK+R
Sbjct: 493 KESSRKK-KKKSKKNKEVDEDDEEEGTSTKKKKKKRR 520

[Close]

BLAST of MS021309 vs. TAIR 10
Match: AT4G00060.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 117.5 bits (293), Expect = 3.4e-26
Identity = 94/350 (26.86%), Postives = 152/350 (43.43%), Query Frame = 0


Query: 123  LHKEIVDFCEFLSPTGEERDER-DRAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDI 182
            L+ EI  FC+ ++     R    + A++RV   ++ +WP  +  +FGS  TGL LP+SD+
Sbjct: 1111 LNDEIDSFCKQVAAENMARKPYINWAIKRVTRSLQVLWPRSRTNIFGSSATGLSLPSSDV 1170

Query: 183  DVV-----------ILGSGIPKPQLG-----LQALSRALSQKGIAK--KIQVIGKARVPI 242
            D+V           I  +GI + + G     LQ  +R L+ +   K   ++ +    +PI
Sbjct: 1171 DLVVCLPPVRNLEPIKEAGILEGRNGIKETCLQHAARYLANQEWVKTDSLKTVENTAIPI 1230

Query: 243  IKFVEK----------------------------------------------------QS 302
            I  V +                                                      
Sbjct: 1231 IMLVVEVPCDLICSIQSPKDGPDCITVDQDSNGNTEMVGFEDSAAANSLPTNTGNLAIAK 1290

Query: 303  GIAFDISFDV--QNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYA 362
             +  DISF      G +    +K   +++P   PL L+LK FL  R L++ YSGGL SY 
Sbjct: 1291 SVRLDISFKTPSHTGLQTTQLVKDLTEQFPAATPLALVLKQFLADRTLDQSYSGGLSSYC 1350

Query: 363  LLTMLMAMLQSHNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRG 400
            L+ ++   LQ  +    S+  NLG LL++F  F+G   +   + VS  G GI+ ++ +RG
Sbjct: 1351 LVLLITRFLQHEHHLGRSINQNLGGLLMDFLYFFGNVFDPRQMRVSVQGSGIYRNR-ERG 1410

[Close]

BLAST of MS021309 vs. TAIR 10
Match: AT2G45620.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 85.5 bits (210), Expect = 1.4e-16
Identity = 66/228 (28.95%), Postives = 109/228 (47.81%), Query Frame = 0


Query: 115 RFKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHIWPHCKVEVFGSFQTGL 174
           R+ +  + ++K ++       P  EE +++ + +  + ++V   WPH K+ ++GS     
Sbjct: 433 RYDATFIAIYKSLI-------PAEEELEKQRQLMAHLENLVAKEWPHAKLYLYGSCANSF 492

Query: 175 YLPTSDIDV--VILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSG 234
             P SDIDV   I G  I K ++ L  L+  L    + + +Q + +ARVPI+K ++  +G
Sbjct: 493 GFPKSDIDVCLAIEGDDINKSEM-LLKLAEILESDNL-QNVQALTRARVPIVKLMDPVTG 552

Query: 235 IAFDISFD----VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSY 294
           I+ DI  +    V N     DY ++ V     LR L  I+K + + R +NE Y G L SY
Sbjct: 553 ISCDICINNVLAVVNTKLLRDYAQIDV----RLRQLAFIVKHWAKSRRVNETYQGTLSSY 612

Query: 295 ALLTMLMAMLQSHN----SCQSSLEHNLGVLLVN----FFDFYGRKLN 329
           A + M +  LQ        C   +E    V + N    +FD   R  N
Sbjct: 613 AYVLMCIHFLQQRRPPILPCLQEMEPTYSVRVDNIRCTYFDNVDRLRN 647

[Close]

BLAST of MS021309 vs. TAIR 10
Match: AT2G39740.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 4.1e-11
Identity = 53/195 (27.18%), Postives = 93/195 (47.69%), Query Frame = 0


Query: 116 FKSPMLQLHKEIVDFCEFLSPTGEERDERDRAVERVFSVVKHI--WPHCKVEVFGSFQTG 175
           F  P LQ      +  + + PT  +RD R   ++++  V++ +       V+ FGSF + 
Sbjct: 6   FLDPTLQ------EILQVIKPTRADRDTRITVIDQLRDVLQSVECLRGATVQPFGSFVSN 65

Query: 176 LYLPTSDIDV--------VILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIK 235
           L+    D+D+         IL +G  + Q  L  L RAL   G+  K+Q +  ARVPI+K
Sbjct: 66  LFTRWGDLDISVDLFSGSSILFTGKKQKQTLLGHLLRALRASGLWYKLQFVIHARVPILK 125

Query: 236 FVEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGL 295
            V     I+ DIS D  +G   + ++    +     R L L++K + +   +N+  +G  
Sbjct: 126 VVSGHQRISCDISIDNLDGLLKSRFLFWISEIDGRFRDLVLLVKEWAKAHNINDSKTGTF 185

Query: 296 GSYALLTMLMAMLQS 301
            SY+L  +++   Q+
Sbjct: 186 NSYSLSLLVIFHFQT 194

[Close]

BLAST of MS021309 vs. TAIR 10
Match: AT3G45750.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 9.0e-11
Identity = 52/199 (26.13%), Postives = 100/199 (50.25%), Query Frame = 0


Query: 120 MLQLHKEIVD-FCEFLSPTGEERDERDRAVERVFSVVKHIWPHCK-----VEVFGSFQTG 179
           +L L K + D +C F  P   + + R   V+ + ++   I+   +     +E +GSF   
Sbjct: 40  LLDLDKVLNDVYCSF-RPVSADYNTRKELVKNLNTMALDIYGKSEESSPVLEAYGSFVMD 99

Query: 180 LYLPTSDIDVVI-LGSG---IPKPQLGLQALS------RALSQKGIAKKIQVIGKARVPI 239
           +Y   SD+DV I  G+G   IP+ +  L+ L       R+L  +G  K ++ I  A+VPI
Sbjct: 100 MYSSQSDLDVSINFGNGTSEIPR-EKKLEILKRFAKKLRSLQGEGQVKNVESIFSAKVPI 159

Query: 240 IKFVEKQSGIAFDISFDVQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSG 299
           +KF ++ +G+  D+S + ++G   +  +++        + LCL++K + +  E+N     
Sbjct: 160 VKFSDQGTGVECDLSVENKDGILNSQIVRIISQIDGRFQKLCLLVKHWAKAHEVNSALHR 219

Query: 300 GLGSYALLTMLMAMLQSHN 303
            L S ++  ++   LQ+ N
Sbjct: 220 TLNSVSITLLVALHLQTQN 236

The following BLAST results are available for this feature:

BLAST of MS021309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139704.1	3.5e-307	99.26	non-canonical poly(A) RNA polymerase PAPD5 [Momordica charantia]	[more]
XP_038899128.1	9.0e-263	87.25	terminal nucleotidyltransferase 4B [Benincasa hispida]	[more]
XP_022936827.1	2.5e-249	84.38	non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata] >XP_022936828.1 ...	[more]
XP_023535086.1	3.3e-249	84.38	non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo] >XP_0235...	[more]
XP_022976478.1	3.3e-249	84.38	non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima] >XP_022976479.1 no...	[more]

BLAST of MS021309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q68ED3	3.0e-59	38.92	Terminal nucleotidyltransferase 4B OS=Mus musculus OX=10090 GN=Tent4b PE=1 SV=2	[more]
Q7KVS9	1.1e-58	41.35	Non-canonical poly(A) RNA polymerase protein Trf4-1 OS=Drosophila melanogaster O...	[more]
Q5XG87	1.9e-58	37.12	Terminal nucleotidyltransferase 4A OS=Homo sapiens OX=9606 GN=TENT4A PE=1 SV=3	[more]
Q8NDF8	4.9e-54	35.24	Terminal nucleotidyltransferase 4B OS=Homo sapiens OX=9606 GN=TENT4B PE=1 SV=2	[more]
Q6PB75	1.4e-53	41.18	Terminal nucleotidyltransferase 4A OS=Mus musculus OX=10090 GN=Tent4a PE=2 SV=2	[more]

BLAST of MS021309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDS2	1.7e-307	99.26	non-canonical poly(A) RNA polymerase PAPD5 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1F9G2	1.2e-249	84.38	non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IMA1	1.6e-249	84.38	non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0L271	7.5e-247	83.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637740 PE=4 SV=1	[more]
A0A1S3BTA1	1.3e-246	83.27	non-canonical poly(A) RNA polymerase PAPD5 OS=Cucumis melo OX=3656 GN=LOC1034934...	[more]

BLAST of MS021309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53770.1	6.4e-166	61.90	Nucleotidyltransferase family protein	[more]
AT4G00060.1	3.4e-26	26.86	Nucleotidyltransferase family protein	[more]
AT2G45620.1	1.4e-16	28.95	Nucleotidyltransferase family protein	[more]
AT2G39740.1	4.1e-11	27.18	Nucleotidyltransferase family protein	[more]
AT3G45750.1	9.0e-11	26.13	Nucleotidyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043519	Nucleotidyltransferase superfamily	GENE3D	3.30.460.10	Beta Polymerase, domain 2	coord: 136..244 e-value: 3.9E-98 score: 330.3
IPR043519	Nucleotidyltransferase superfamily	SUPERFAMILY	81301	Nucleotidyltransferase	coord: 119..271
IPR002058	PAP/25A-associated	PFAM	PF03828	PAP_assoc	coord: 311..369 e-value: 8.3E-14 score: 51.6
None	No IPR available	GENE3D	1.10.1410.10		coord: 118..394 e-value: 3.9E-98 score: 330.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 459..543
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..102
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 491..525
None	No IPR available	PANTHER	PTHR23092:SF15	INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED	coord: 100..532
None	No IPR available	PANTHER	PTHR23092	POLY(A) RNA POLYMERASE	coord: 100..532
None	No IPR available	CDD	cd05402	NT_PAP_TUTase	coord: 144..252 e-value: 4.5484E-36 score: 128.444
None	No IPR available	SUPERFAMILY	81631	PAP/OAS1 substrate-binding domain	coord: 259..397
IPR002934	Polymerase, nucleotidyl transferase domain	PFAM	PF01909	NTP_transf_2	coord: 150..239 e-value: 5.7E-11 score: 42.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021309.1	MS021309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016779	nucleotidyltransferase activity