MS021280 (gene) Bitter gourd (TR) v1

Overview


Name	MS021280
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ethylene-responsive transcription factor 4
Location	scaffold358: 698948 .. 699529 (+)
RNA-Seq Expression	MS021280
Synteny	MS021280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCGGAGATCGGCCGACATCCTCCTCCGCCGCCGTGACTCCCGAGATCCGTTACCGCGGCGTCAGGAAGAGGCCGTGGGGCCGTTACGCCGCGGAAATCCGGGACCCCCGTAAGAAGACTCGCGTTTGGCTCGGCACCTTCAACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCTGCGCGTGAATTCCGAGGCGCTAACGCCAAAACCAACTTCCCCGTTCCCGCCGAGTTCGCCGCCCTCGTCGGCGAAAATCACTACCGTAAATCGGCCTGCAGTAGTCCCAGCCAGAGCAGCACCGTCGACTCCTCCTCCCCGTCGCCGGCGCCGCCACCCGTGGATCCGGCTCTCACTCACTTCATCGACGGCGGCGCCATTTCACGCCCAATTTACTTCTTCGACGCGTTCGCCCAGGCGGAGAAGACGAGTGACTTTCACCGGACCGTCACCGTCGCCGTTGCCGGCGGATCAGCGAGTGACTCTGATTCGTCCTCTGTAGTAGTTGATTTCAACCATACTCGGAAACCGCGGCCGTTGGATTTCGACCTGAATCTTCCGGCAGTAGAAGTTGCG

mRNA sequence

ATGGCTGCCGGAGATCGGCCGACATCCTCCTCCGCCGCCGTGACTCCCGAGATCCGTTACCGCGGCGTCAGGAAGAGGCCGTGGGGCCGTTACGCCGCGGAAATCCGGGACCCCCGTAAGAAGACTCGCGTTTGGCTCGGCACCTTCAACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCTGCGCGTGAATTCCGAGGCGCTAACGCCAAAACCAACTTCCCCGTTCCCGCCGAGTTCGCCGCCCTCGTCGGCGAAAATCACTACCGTAAATCGGCCTGCAGTAGTCCCAGCCAGAGCAGCACCGTCGACTCCTCCTCCCCGTCGCCGGCGCCGCCACCCGTGGATCCGGCTCTCACTCACTTCATCGACGGCGGCGCCATTTCACGCCCAATTTACTTCTTCGACGCGTTCGCCCAGGCGGAGAAGACGAGTGACTTTCACCGGACCGTCACCGTCGCCGTTGCCGGCGGATCAGCGAGTGACTCTGATTCGTCCTCTGTAGTAGTTGATTTCAACCATACTCGGAAACCGCGGCCGTTGGATTTCGACCTGAATCTTCCGGCAGTAGAAGTTGCG

Coding sequence (CDS)

ATGGCTGCCGGAGATCGGCCGACATCCTCCTCCGCCGCCGTGACTCCCGAGATCCGTTACCGCGGCGTCAGGAAGAGGCCGTGGGGCCGTTACGCCGCGGAAATCCGGGACCCCCGTAAGAAGACTCGCGTTTGGCTCGGCACCTTCAACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCTGCGCGTGAATTCCGAGGCGCTAACGCCAAAACCAACTTCCCCGTTCCCGCCGAGTTCGCCGCCCTCGTCGGCGAAAATCACTACCGTAAATCGGCCTGCAGTAGTCCCAGCCAGAGCAGCACCGTCGACTCCTCCTCCCCGTCGCCGGCGCCGCCACCCGTGGATCCGGCTCTCACTCACTTCATCGACGGCGGCGCCATTTCACGCCCAATTTACTTCTTCGACGCGTTCGCCCAGGCGGAGAAGACGAGTGACTTTCACCGGACCGTCACCGTCGCCGTTGCCGGCGGATCAGCGAGTGACTCTGATTCGTCCTCTGTAGTAGTTGATTTCAACCATACTCGGAAACCGCGGCCGTTGGATTTCGACCTGAATCTTCCGGCAGTAGAAGTTGCG

Protein sequence

MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALTHFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPRPLDFDLNLPAVEVA

Homology

[Close]

BLAST of MS021280 vs. NCBI nr
Match: XP_022139606.1 (ethylene-responsive transcription factor 4 [Momordica charantia])

HSP 1 Score: 378.6 bits (971), Expect = 3.2e-101
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120
           TAAREFRG NAKTNFPVPAEFA LVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL
Sbjct: 61  TAAREFRGGNAKTNFPVPAEFAVLVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120

Query: 121 THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR 180
           THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR
Sbjct: 121 THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR 180

Query: 181 PLDFDLNLPAVEVA 195
           PLDFDLNLPAVEVA
Sbjct: 181 PLDFDLNLPAVEVA 194

[Close]

BLAST of MS021280 vs. NCBI nr
Match: XP_022981946.1 (ethylene-responsive transcription factor 4 [Cucurbita maxima])

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-70
Identity = 151/197 (76.65%), Postives = 163/197 (82.74%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDR TS++     EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTAVV---EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120
           TAAREFRGANAKTNFP P E+      N +R+S CSSPS+SSTVDSSS   AP P D +L
Sbjct: 61  TAAREFRGANAKTNFPTPNEYLVDDNNNKFRRSTCSSPSESSTVDSSS---APSPTDASL 120

Query: 121 THFIDGG---AISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTR 180
           T F+DG    AISRPI+FFDAFAQAEK SDFHRTVTVAVAGGSASDSDSSSVV   + ++
Sbjct: 121 TRFLDGSHPPAISRPIFFFDAFAQAEKMSDFHRTVTVAVAGGSASDSDSSSVVDFDDDSQ 180

Query: 181 KPRPLDFDLNLPAVEVA 195
           K RP DFDLNLPAVEVA
Sbjct: 181 KSRPFDFDLNLPAVEVA 191

[Close]

BLAST of MS021280 vs. NCBI nr
Match: KAG6607891.1 (Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.1 bits (697), Expect = 1.9e-69
Identity = 150/197 (76.14%), Postives = 164/197 (83.25%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDR TS++A    EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTAAV---EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120
           +AAREFRGANAKTNFP P E+      N +R+SACSSPS+SSTVDSSS   AP P D +L
Sbjct: 61  SAAREFRGANAKTNFPTPNEYLVDDNNNKFRRSACSSPSESSTVDSSS---APSPTDASL 120

Query: 121 THFIDGG---AISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTR 180
           T F+DG    AISRPI+FFDAFAQAEK +DF RTVTVAVAGGSASDSDSSSVV   + ++
Sbjct: 121 TRFLDGSHPPAISRPIFFFDAFAQAEKMNDFRRTVTVAVAGGSASDSDSSSVVDFDDDSQ 180

Query: 181 KPRPLDFDLNLPAVEVA 195
           K RP DFDLNLPAVEVA
Sbjct: 181 KSRPFDFDLNLPAVEVA 191

[Close]

BLAST of MS021280 vs. NCBI nr
Match: XP_022941385.1 (ethylene-responsive transcription factor 4 [Cucurbita moschata] >KAG7037418.1 Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 270.4 bits (690), Expect = 1.2e-68
Identity = 152/200 (76.00%), Postives = 168/200 (84.00%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDR TS++A    EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTAAV---EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENH---YRKSACSSPSQSSTVDSSSPSPAPPPVD 120
           +AAREFRGANAKTNFP P E+  LV +N+   +R+SACSSPS+SSTVDSSS   AP P D
Sbjct: 61  SAAREFRGANAKTNFPTPNEY--LVDDNNNNKFRRSACSSPSESSTVDSSS---APSPTD 120

Query: 121 PALTHFIDGG---AISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFN 180
            +LT F+DG    AISRPI+FFDAFAQAEK +DF RTVTVAVAGGSASDSDSSSVV   +
Sbjct: 121 ASLTRFLDGSHPPAISRPIFFFDAFAQAEKMNDFRRTVTVAVAGGSASDSDSSSVVDFDD 180

Query: 181 HTRKPRPLDFDLNLPAVEVA 195
            ++K RP DFDLNLPAVEVA
Sbjct: 181 DSQKSRPFDFDLNLPAVEVA 192

[Close]

BLAST of MS021280 vs. NCBI nr
Match: XP_038897001.1 (ethylene-responsive transcription factor 4 [Benincasa hispida])

HSP 1 Score: 268.9 bits (686), Expect = 3.5e-68
Identity = 152/204 (74.51%), Postives = 166/204 (81.37%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTP----EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           M AGDR TS+++   P    EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MVAGDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPVPAEFAALVGENHYRKSACS-SPSQSSTVDSSSPSPAPPP 120
           RAYDTAAREFRGANAKTNFP+  EF  +   N++ KSACS SPSQSSTVDSSSPSPAP  
Sbjct: 61  RAYDTAAREFRGANAKTNFPIQNEFLMVDNNNNFHKSACSTSPSQSSTVDSSSPSPAPS- 120

Query: 121 VDPALTHFIDGG----AISRPIYFFDAFAQAEKTSDFHRTVT--VAVAGGSASDSDSSSV 180
            DP+LTHF++       ISRPI+FFDAFAQAE+ S+F R VT  VA AGGS SDSDSSS 
Sbjct: 121 -DPSLTHFLNSSNHAPTISRPIFFFDAFAQAERISEFRRNVTNAVAGAGGSVSDSDSSS- 180

Query: 181 VVDFNHTRKPRPLDFDLNLPAVEV 194
           VVDF+ TRK RPLDFDLNLPAVEV
Sbjct: 181 VVDFDTTRKSRPLDFDLNLPAVEV 201

[Close]

BLAST of MS021280 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.0e-35
Identity = 104/200 (52.00%), Postives = 117/200 (58.50%), Query Frame = 0


Query: 17  EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFP 76
           EIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAR+FRGA AKTNFP
Sbjct: 22  EIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKTNFP 81

Query: 77  VPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPP------VDPALTHFIDGGA-- 136
              E    + +         SPSQSST+D +SP     P      V P L   + GG   
Sbjct: 82  TFLE----LSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLELSLGGGGGG 141

Query: 137 ------ISRPIYFFDAF-------AQAEKTSDFHRTVTVAVAG----GSASDSDSSSVVV 190
                 +SRP+YF D          Q    +   R+  V VA     G+ SDSDSSS VV
Sbjct: 142 SCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGAQSDSDSSS-VV 201

[Close]

BLAST of MS021280 vs. ExPASy Swiss-Prot
Match: Q9MAI5 (Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ERF8 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-28
Identity = 92/195 (47.18%), Postives = 111/195 (56.92%), Query Frame = 0


Query: 3   AGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTA 62
           A   PT   +    EIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYD A
Sbjct: 14  APTNPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAA 73

Query: 63  AREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALTH 122
           AR+FRG  AKTNF V      +VG         SSP+QSSTV   SP+ A     P L  
Sbjct: 74  ARDFRGVKAKTNFGV------IVG---------SSPTQSSTV-VDSPTAARFITPPHLEL 133

Query: 123 FIDGGAISR-------PIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDF-- 182
            + GG   R       P+Y+++     + T+            G  S+S++SS VVDF  
Sbjct: 134 SLGGGGACRRKIPLVHPVYYYNMATYPKMTT-----------CGVQSESETSS-VVDFEG 180

Query: 183 NHTRKPRPLDFDLNL 189
              +   PLD DLNL
Sbjct: 194 GAGKISPPLDLDLNL 180

[Close]

BLAST of MS021280 vs. ExPASy Swiss-Prot
Match: Q9LW49 (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ERF4 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 87/209 (41.63%), Postives = 115/209 (55.02%), Query Frame = 0


Query: 17  EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFP 76
           E+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYDTAAREFRG  AKTNFP
Sbjct: 24  EVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNFP 83

Query: 77  VPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALTHFI-----DGGAISR 136
           +P+E          + ++ SS  +SS+ ++   +P   P++  LT  +     DGG   R
Sbjct: 84  LPSE---------NQSTSHSSTMESSSGETGIHAPPHAPLELDLTRRLGSVAADGGDNCR 143

Query: 137 -----------------------PIYFFDAF---AQAEKTSDFHRTVTVAV------AGG 189
                                  P+  FD+        +   +H  + +A+      AG 
Sbjct: 144 RSGEVGYPIFHQQPTVAVLPNGQPVLLFDSLWRPGVVNRPQPYH-VMPMAMGFNGVNAGV 203

[Close]

BLAST of MS021280 vs. ExPASy Swiss-Prot
Match: Q40477 (Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 73/121 (60.33%), Postives = 81/121 (66.94%), Query Frame = 0


Query: 17  EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFP 76
           E+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYDTAAREFRG  AKTNFP
Sbjct: 24  EVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNFP 83

Query: 77  VPAEFAALVGENHYRKSACSSPSQSSTVDSSSPS---PAPP--PVDPALTHFI-----DG 128
            P E                SPS SSTV+SSS      APP  P++  LT  +     DG
Sbjct: 84  SPTE--------------NQSPSHSSTVESSSGENGVHAPPHAPLELDLTRRLGSVAADG 130

[Close]

BLAST of MS021280 vs. ExPASy Swiss-Prot
Match: Q9FE67 (Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ERF9 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 83/197 (42.13%), Postives = 106/197 (53.81%), Query Frame = 0


Query: 4   GDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 63
           G   T +   +  E+ +RGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAA
Sbjct: 17  GGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAA 76

Query: 64  REFRGANAKTNFPVPAEFAAL--VGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALT 123
           REFRG+ AKTNFP+P E   +   GEN       S  ++++   +   +    P      
Sbjct: 77  REFRGSKAKTNFPLPGESTTVNDGGEND------SYVNRTTVTTAREMTRQRFPFACHRE 136

Query: 124 HFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPRP 183
             + GG  S   +FFD    A   ++  R   V        D  +  + +    T K  P
Sbjct: 137 RKVVGGYASAG-FFFDPSRAASLRAELSRVCPVRF------DPVNIELSIGIRETVKVEP 196

Query: 184 ---LDFDLNL--PAVEV 194
              L+ DLNL  P V+V
Sbjct: 197 RRELNLDLNLAPPVVDV 200

[Close]

BLAST of MS021280 vs. ExPASy TrEMBL
Match: A0A6J1CD65 (ethylene-responsive transcription factor 4 OS=Momordica charantia OX=3673 GN=LOC111010466 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.5e-101
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120
           TAAREFRG NAKTNFPVPAEFA LVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL
Sbjct: 61  TAAREFRGGNAKTNFPVPAEFAVLVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120

Query: 121 THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR 180
           THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR
Sbjct: 121 THFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPR 180

Query: 181 PLDFDLNLPAVEVA 195
           PLDFDLNLPAVEVA
Sbjct: 181 PLDFDLNLPAVEVA 194

[Close]

BLAST of MS021280 vs. ExPASy TrEMBL
Match: A0A6J1IXY7 (ethylene-responsive transcription factor 4 OS=Cucurbita maxima OX=3661 GN=LOC111480939 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 151/197 (76.65%), Postives = 163/197 (82.74%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDR TS++     EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTAVV---EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPAL 120
           TAAREFRGANAKTNFP P E+      N +R+S CSSPS+SSTVDSSS   AP P D +L
Sbjct: 61  TAAREFRGANAKTNFPTPNEYLVDDNNNKFRRSTCSSPSESSTVDSSS---APSPTDASL 120

Query: 121 THFIDGG---AISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTR 180
           T F+DG    AISRPI+FFDAFAQAEK SDFHRTVTVAVAGGSASDSDSSSVV   + ++
Sbjct: 121 TRFLDGSHPPAISRPIFFFDAFAQAEKMSDFHRTVTVAVAGGSASDSDSSSVVDFDDDSQ 180

Query: 181 KPRPLDFDLNLPAVEVA 195
           K RP DFDLNLPAVEVA
Sbjct: 181 KSRPFDFDLNLPAVEVA 191

[Close]

BLAST of MS021280 vs. ExPASy TrEMBL
Match: A0A6J1FTH9 (ethylene-responsive transcription factor 4 OS=Cucurbita moschata OX=3662 GN=LOC111446690 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 5.8e-69
Identity = 152/200 (76.00%), Postives = 168/200 (84.00%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60
           MAAGDR TS++A    EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD
Sbjct: 1   MAAGDRSTSTAAV---EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 60

Query: 61  TAAREFRGANAKTNFPVPAEFAALVGENH---YRKSACSSPSQSSTVDSSSPSPAPPPVD 120
           +AAREFRGANAKTNFP P E+  LV +N+   +R+SACSSPS+SSTVDSSS   AP P D
Sbjct: 61  SAAREFRGANAKTNFPTPNEY--LVDDNNNNKFRRSACSSPSESSTVDSSS---APSPTD 120

Query: 121 PALTHFIDGG---AISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFN 180
            +LT F+DG    AISRPI+FFDAFAQAEK +DF RTVTVAVAGGSASDSDSSSVV   +
Sbjct: 121 ASLTRFLDGSHPPAISRPIFFFDAFAQAEKMNDFRRTVTVAVAGGSASDSDSSSVVDFDD 180

Query: 181 HTRKPRPLDFDLNLPAVEVA 195
            ++K RP DFDLNLPAVEVA
Sbjct: 181 DSQKSRPFDFDLNLPAVEVA 192

[Close]

BLAST of MS021280 vs. ExPASy TrEMBL
Match: A0A6J1IML9 (ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111476936 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 9.3e-67
Identity = 150/197 (76.14%), Postives = 166/197 (84.26%), Query Frame = 0


Query: 1   MAAGDRPTSS-SAAV---TPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAA DR TS+ SAAV   + EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAANDRSTSTVSAAVASNSEEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPV 120
           RAYDTAAREFRGA+AKTNFPVP +F  +   N++RKSACS+PSQ STV+SSSPSPA  P+
Sbjct: 61  RAYDTAAREFRGADAKTNFPVPNDFQIIDINNNFRKSACSNPSQCSTVESSSPSPA--PL 120

Query: 121 DPALTHFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHT 180
           DPALT ++   +ISRPI+FFDAFAQ EK SDF R   V+VAG S SDSDSSS VVDF+ T
Sbjct: 121 DPALT-YVHAPSISRPIFFFDAFAQVEKNSDFRR--IVSVAGRSRSDSDSSS-VVDFDRT 180

Query: 181 RKPRPLDFDLNLPAVEV 194
           RK RPLDFDLNLPAVEV
Sbjct: 181 RKSRPLDFDLNLPAVEV 191

[Close]

BLAST of MS021280 vs. ExPASy TrEMBL
Match: A0A6J1F5I9 (ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111442554 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.0e-65
Identity = 146/197 (74.11%), Postives = 161/197 (81.73%), Query Frame = 0


Query: 1   MAAGDRPTSSSAAV----TPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAAGD+ TS+++A     + EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGT NTAEEAA
Sbjct: 1   MAAGDQSTSTASAAEASNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTINTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPV 120
           RAYDTAAREFRGA+AKTNFPVP  F  +   N++RKSACSSPSQ STV+S+SPSPAP   
Sbjct: 61  RAYDTAAREFRGADAKTNFPVPNGFLTVDNNNNFRKSACSSPSQCSTVESTSPSPAPS-- 120

Query: 121 DPALTHFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHT 180
           DP +T  +   AISRPI+FFDAFAQ EK SDF R VT   AGGS SDSDSSS VVDF+ T
Sbjct: 121 DPTITD-VHAPAISRPIFFFDAFAQVEKNSDFRRIVT--GAGGSRSDSDSSS-VVDFDRT 180

Query: 181 RKPRPLDFDLNLPAVEV 194
           RK RPLDFDLNLPAVEV
Sbjct: 181 RKSRPLDFDLNLPAVEV 191

[Close]

BLAST of MS021280 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 148.7 bits (374), Expect = 4.9e-36
Identity = 104/200 (52.00%), Postives = 117/200 (58.50%), Query Frame = 0


Query: 17  EIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFP 76
           EIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAR+FRGA AKTNFP
Sbjct: 22  EIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKTNFP 81

Query: 77  VPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPP------VDPALTHFIDGGA-- 136
              E    + +         SPSQSST+D +SP     P      V P L   + GG   
Sbjct: 82  TFLE----LSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLELSLGGGGGG 141

Query: 137 ------ISRPIYFFDAF-------AQAEKTSDFHRTVTVAVAG----GSASDSDSSSVVV 190
                 +SRP+YF D          Q    +   R+  V VA     G+ SDSDSSS VV
Sbjct: 142 SCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGAQSDSDSSS-VV 201

[Close]

BLAST of MS021280 vs. TAIR 10
Match: AT1G53170.1 (ethylene response factor 8 )

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-29
Identity = 92/195 (47.18%), Postives = 111/195 (56.92%), Query Frame = 0


Query: 3   AGDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTA 62
           A   PT   +    EIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYD A
Sbjct: 14  APTNPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAA 73

Query: 63  AREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALTH 122
           AR+FRG  AKTNF V      +VG         SSP+QSSTV   SP+ A     P L  
Sbjct: 74  ARDFRGVKAKTNFGV------IVG---------SSPTQSSTV-VDSPTAARFITPPHLEL 133

Query: 123 FIDGGAISR-------PIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDF-- 182
            + GG   R       P+Y+++     + T+            G  S+S++SS VVDF  
Sbjct: 134 SLGGGGACRRKIPLVHPVYYYNMATYPKMTT-----------CGVQSESETSS-VVDFEG 180

Query: 183 NHTRKPRPLDFDLNL 189
              +   PLD DLNL
Sbjct: 194 GAGKISPPLDLDLNL 180

[Close]

BLAST of MS021280 vs. TAIR 10
Match: AT5G44210.1 (erf domain protein 9 )

HSP 1 Score: 114.8 bits (286), Expect = 7.9e-26
Identity = 83/197 (42.13%), Postives = 106/197 (53.81%), Query Frame = 0


Query: 4   GDRPTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 63
           G   T +   +  E+ +RGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAA
Sbjct: 17  GGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAA 76

Query: 64  REFRGANAKTNFPVPAEFAAL--VGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALT 123
           REFRG+ AKTNFP+P E   +   GEN       S  ++++   +   +    P      
Sbjct: 77  REFRGSKAKTNFPLPGESTTVNDGGEND------SYVNRTTVTTAREMTRQRFPFACHRE 136

Query: 124 HFIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVDFNHTRKPRP 183
             + GG  S   +FFD    A   ++  R   V        D  +  + +    T K  P
Sbjct: 137 RKVVGGYASAG-FFFDPSRAASLRAELSRVCPVRF------DPVNIELSIGIRETVKVEP 196

Query: 184 ---LDFDLNL--PAVEV 194
              L+ DLNL  P V+V
Sbjct: 197 RRELNLDLNLAPPVVDV 200

[Close]

BLAST of MS021280 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 113.2 bits (282), Expect = 2.3e-25
Identity = 86/188 (45.74%), Postives = 104/188 (55.32%), Query Frame = 0


Query: 7   PTSSSAAVTPE----IRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTA 66
           PT  +AAV       +RYRGVRKRPWGRYAAEIRDP KK+RVWLGTF+T EEAARAYD  
Sbjct: 3   PTVKTAAVKTNEGNGVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKR 62

Query: 67  AREFRGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPPPVDPALTH 126
           A EFRGA AKTNFP           N +  S   S SQSSTV+SS P+            
Sbjct: 63  AIEFRGAKAKTNFP-------CYNINAHCLSLTQSLSQSSTVESSFPN------------ 122

Query: 127 FIDGGAISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSASDSDSSSVVVD-FNHTRKPRP 186
            ++ G+ S    F   F + +  +         +     S+SDSSSVV+D   +  +   
Sbjct: 123 -LNLGSDSVSSRF--PFPKIQVKAGM-------MVFDERSESDSSSVVMDVVRYEGRRVV 161

Query: 187 LDFDLNLP 190
           LD DLN P
Sbjct: 183 LDLDLNFP 161

[Close]

BLAST of MS021280 vs. TAIR 10
Match: AT3G20310.1 (ethylene response factor 7 )

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-24
Identity = 86/228 (37.72%), Postives = 111/228 (48.68%), Query Frame = 0


Query: 7   PTSSSAAVTPEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREF 66
           P ++  +V  E RYRGVRKRPWGR+AAEIRDP KK+RVWLGTF++A +AARAYDTAAR  
Sbjct: 15  PVTAGGSV-KEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTFDSAVDAARAYDTAARNL 74

Query: 67  RGANAKTNFPVPAEFAALVGENHYRKSACSSPSQSSTVDSSSPSPAPP----PVDPALTH 126
           RG  AKTNFP+                + SSP Q  T   +    +PP     +DP + H
Sbjct: 75  RGPKAKTNFPIDC--------------SPSSPLQPLTYLHNQNLCSPPVIQNQIDPFMDH 134

Query: 127 FIDGGA----------ISRPIYFFDAFAQAEKTSDFHRTVTVAVAGGSA----------- 186
            + GG           ISRP     + +   K+    R +  A A  S            
Sbjct: 135 RLYGGGNFQEQQQQQIISRPA--SSSMSSTVKSCSGPRPMEAAAASSSVAKPLHAIKRYP 194

Query: 187 -----------SDSDSSSVVVD------FNHTRKPRPLDFDLNLPAVE 193
                      SD DSSS V+D       + +R+  P  FDLN P ++
Sbjct: 195 RTPPVAPEDCHSDCDSSSSVIDDGDDIASSSSRRKTPFQFDLNFPPLD 225

The following BLAST results are available for this feature:

BLAST of MS021280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139606.1	3.2e-101	98.97	ethylene-responsive transcription factor 4 [Momordica charantia]	[more]
XP_022981946.1	2.9e-70	76.65	ethylene-responsive transcription factor 4 [Cucurbita maxima]	[more]
KAG6607891.1	1.9e-69	76.14	Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subs...	[more]
XP_022941385.1	1.2e-68	76.00	ethylene-responsive transcription factor 4 [Cucurbita moschata] >KAG7037418.1 Et...	[more]
XP_038897001.1	3.5e-68	74.51	ethylene-responsive transcription factor 4 [Benincasa hispida]	[more]

BLAST of MS021280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80340	7.0e-35	52.00	Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9MAI5	3.7e-28	47.18	Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9LW49	1.0e-25	41.63	Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ER...	[more]
Q40477	1.7e-25	60.33	Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 ...	[more]
Q9FE67	1.1e-24	42.13	Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]

BLAST of MS021280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CD65	1.5e-101	98.97	ethylene-responsive transcription factor 4 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1IXY7	1.4e-70	76.65	ethylene-responsive transcription factor 4 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1FTH9	5.8e-69	76.00	ethylene-responsive transcription factor 4 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IML9	9.3e-67	76.14	ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1F5I9	1.0e-65	74.11	ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MS021280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15210.1	4.9e-36	52.00	ethylene responsive element binding factor 4	[more]
AT1G53170.1	2.6e-29	47.18	ethylene response factor 8	[more]
AT5G44210.1	7.9e-26	42.13	erf domain protein 9	[more]
AT1G28370.1	2.3e-25	45.74	ERF domain protein 11	[more]
AT3G20310.1	1.5e-24	37.72	ethylene response factor 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 20..31 score: 66.67 coord: 42..58 score: 74.65
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 19..82 e-value: 1.7E-36 score: 137.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 19..68 e-value: 3.9E-14 score: 52.6
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 19..76 score: 24.539518
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 19..77 e-value: 9.89789E-30 score: 102.35
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 18..77 e-value: 1.7E-28 score: 100.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 92..107
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 92..120
None	No IPR available	PANTHER	PTHR31677:SF156	ERF FAMILY PROTEIN	coord: 7..117
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 127..183
None	No IPR available	PANTHER	PTHR31677:SF156	ERF FAMILY PROTEIN	coord: 127..183
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 7..117
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 19..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021280.1	MS021280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity