MS021276 (gene) Bitter gourd (TR) v1

Overview
NameMS021276
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmediator-associated protein 2
Locationscaffold358: 673116 .. 675110 (-)
RNA-Seq ExpressionMS021276
SyntenyMS021276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAACGAATTTGGTGCTTTTAAAAGGGCAGAAGAATACAAACCCGCACCAGAGTTTGAGGAAGACGATAGGGAATTGCTTGCTGATATCTCTTTGTCTGACTCCACTGAGCTTTGGCTTATTCAATGGCCTGCTAATCAAGTAAGATCCTACAAACTAATCCTTGAAAACATAATAGCTACCTGCAATTTGGGAGAAAATATAATAAAGAATAGCCCATAAAATGTACCTTCTTTCAAGATTGTAGTTTGTTACAGTACAGCCATTGCATGTGTTTAATTTGGAAAGGATTTATTTTTTTCTTTATTGTAGCCCCCTGATTTTGATGGGCAAGAGTTTTCTCTCCAACTTCATCATGATGGACATTTGGGCAGCTTTCAGGGTTCATCTGGTAATTATAATTTTTTTTGGTGCTATTGTACGCCAACAATCTGATCTCGACATTATTTTCTTTAGCTATTCTGTTCCTTTTCTTACAATATTTTCAATGTATTATTTGTTACCGTCAAAAGGAAAATCGTATGATGTGGTCAGCTGTGTGGCTCAAGAACCCGAGGCTTCTGTTTTTCTACCATCTTCATCAGACACCAAGTTAGGTAAATGCCTCCTATTTCTATTCTAACAAATTTATCTATATTCTGATTTTGTTTCACTTTTACTTCACATGCCAGTAGTATCAGTCTCTCCATTGCAGCTACATTCTCTCTGCCCTTTTTCTTGTTATACTTGGTGGTACTTGCGCTTTTTCAGTTGTACACTCCATTCGTGCTTCAGTTTGAGAGTCCCTATATTTTATATTAGGATTTAGGGCATTGTAGTTTCACTTTTAACACTAATAACAGTAGCGTCTAGATTTCTAGCTTCTCCTTTGTCCCGAGCTAAATCTAATTCTGTATGTTTTCACATGACCGTATAGTTGACGAACTTTTAATCTCCTTATGTCATCAGCATCTGTCGTTGCTGGTTCTGTCTTAAAATTTTATTTGTCAGCTGCGATACTCAAACATTAGATTCAACTTTCTGCTAGGCTCCAATGGAGGAGGTTTTGTTGTTCCCTCATTTTCGGGATAAGGAAAGATTTTTTTTTGGTAGGCTTGTTTCTGTCCGATTTTGTGGGAGATATAGATCAAGAAAAATAATGGAATCTTTAAGGGTGGAGAGATCCAAGGAGTATGTTTTGGAGCTTATTAGATTTAATACTTCCTTGCGTGGTCATTTTATGAATTCTCTTGTAACTATGAGTTAGGTGTTCCTTGTTGGTTGGAGCCCCTTTCTTTAGGTGTGCCTCTAATAGAGCTTTTTTTTTATGCCCTTTTCTAAGCTTTCTTTTTTCATTTTTGTCAATGAAAAGCTTGGTTTTTCATAAATAAAAAAACTTTCTGCATGCCTAAATGTTTCGGAACGAGAACGAACAATGTTGTTCATCTCAATTCAAGTCCAATTGTCTCTTGATGTTTGTTCACATCACATCATAATATTCTAGTGGCATGGTCCTTATTGTATGAGTAGCTTTACCATGCTTTATTTGATAAAATTGTTTCCATTTTGTGATCCTGTTCTTATATCTTTGAAGTTGGAAAGATTTCCCGGCGGATATCTCTCGTGCATTATCCAGAACCTGAAGAACTCGAAAAGGCCACAGCTATGAAATCAGCGTTTCAGAAATCTTCTGGAATTTCGTTGACCACATCTTCTCGTCAAACTACATCCCGAACTCCACACTCGAGCAGTAGAAGAACAAGCTCTAGTAAACCAAGGAGTTCAGTGTCTGAATATGAGGAACCATCAAAGCCTTCAAGTCGAAAACGGGAACCGGAACCTTCCGAGTCCAAAGATCATTTGAAAAAGGATTCATCGAAAAGAAAACACGCACATGAACCTAGTAGATCGACCGACCGGTCGACTCAGAATTTGGGACGTAGTCATAGTGCAGTAACTTTTTCAGGGGCCTCCGGGCAATCTAGCGAAGGTAAAACAAAAAGGCAAAAGAAA

mRNA sequence

ATGAGCAACGAATTTGGTGCTTTTAAAAGGGCAGAAGAATACAAACCCGCACCAGAGTTTGAGGAAGACGATAGGGAATTGCTTGCTGATATCTCTTTGTCTGACTCCACTGAGCTTTGGCTTATTCAATGGCCTGCTAATCAACCCCCTGATTTTGATGGGCAAGAGTTTTCTCTCCAACTTCATCATGATGGACATTTGGGCAGCTTTCAGGGTTCATCTGGAAAATCGTATGATGTGGTCAGCTGTGTGGCTCAAGAACCCGAGGCTTCTGTTTTTCTACCATCTTCATCAGACACCAAGTTAGTTGGAAAGATTTCCCGGCGGATATCTCTCGTGCATTATCCAGAACCTGAAGAACTCGAAAAGGCCACAGCTATGAAATCAGCGTTTCAGAAATCTTCTGGAATTTCGTTGACCACATCTTCTCGTCAAACTACATCCCGAACTCCACACTCGAGCAGTAGAAGAACAAGCTCTAGTAAACCAAGGAGTTCAGTGTCTGAATATGAGGAACCATCAAAGCCTTCAAGTCGAAAACGGGAACCGGAACCTTCCGAGTCCAAAGATCATTTGAAAAAGGATTCATCGAAAAGAAAACACGCACATGAACCTAGTAGATCGACCGACCGGTCGACTCAGAATTTGGGACGTAGTCATAGTGCAGTAACTTTTTCAGGGGCCTCCGGGCAATCTAGCGAAGGTAAAACAAAAAGGCAAAAGAAA

Coding sequence (CDS)

ATGAGCAACGAATTTGGTGCTTTTAAAAGGGCAGAAGAATACAAACCCGCACCAGAGTTTGAGGAAGACGATAGGGAATTGCTTGCTGATATCTCTTTGTCTGACTCCACTGAGCTTTGGCTTATTCAATGGCCTGCTAATCAACCCCCTGATTTTGATGGGCAAGAGTTTTCTCTCCAACTTCATCATGATGGACATTTGGGCAGCTTTCAGGGTTCATCTGGAAAATCGTATGATGTGGTCAGCTGTGTGGCTCAAGAACCCGAGGCTTCTGTTTTTCTACCATCTTCATCAGACACCAAGTTAGTTGGAAAGATTTCCCGGCGGATATCTCTCGTGCATTATCCAGAACCTGAAGAACTCGAAAAGGCCACAGCTATGAAATCAGCGTTTCAGAAATCTTCTGGAATTTCGTTGACCACATCTTCTCGTCAAACTACATCCCGAACTCCACACTCGAGCAGTAGAAGAACAAGCTCTAGTAAACCAAGGAGTTCAGTGTCTGAATATGAGGAACCATCAAAGCCTTCAAGTCGAAAACGGGAACCGGAACCTTCCGAGTCCAAAGATCATTTGAAAAAGGATTCATCGAAAAGAAAACACGCACATGAACCTAGTAGATCGACCGACCGGTCGACTCAGAATTTGGGACGTAGTCATAGTGCAGTAACTTTTTCAGGGGCCTCCGGGCAATCTAGCGAAGGTAAAACAAAAAGGCAAAAGAAA

Protein sequence

MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQLHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEELEKATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKRQKK
Homology
BLAST of MS021276 vs. NCBI nr
Match: XP_022139366.1 (mediator-associated protein 2 [Momordica charantia] >XP_022139367.1 mediator-associated protein 2 [Momordica charantia])

HSP 1 Score: 442.2 bits (1136), Expect = 2.9e-120
Identity = 238/239 (99.58%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ
Sbjct: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE
Sbjct: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120

Query: 121 LEKATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK 180
           LE+ATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK
Sbjct: 121 LEQATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK 180

Query: 181 REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR 240
           REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR
Sbjct: 181 REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR 239

BLAST of MS021276 vs. NCBI nr
Match: XP_038896123.1 (mediator-associated protein 2 [Benincasa hispida])

HSP 1 Score: 332.8 bits (852), Expect = 2.5e-87
Identity = 189/245 (77.14%), Postives = 206/245 (84.08%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           M NE  AF   E YKP  EFEED RE L DISLS+S ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MINEVDAFISVEGYKPPAEFEEDKREFLTDISLSNSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DG LGSFQGSSGKSYDVVSC+AQ PEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGRLGSFQGSSGKSYDVVSCLAQVPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKPS 180
           LEKAT MKS +QKSSG SLT S + TTSR PHSSSRRTS   SSKPRSSVSE+EEPSKPS
Sbjct: 121 LEKATPMKSLYQKSSGTSLTNSRQTTTSRGPHSSSRRTSSSRSSKPRSSVSEFEEPSKPS 180

Query: 181 SRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKT 240
             K+EPEP+ES+D+LKKDSSKR   H+PSRS D ST + GR HSAVT SG++ +SSEGK+
Sbjct: 181 IVKQEPEPTESRDYLKKDSSKRNRTHDPSRSIDHSTPDSGRGHSAVTSSGSTERSSEGKS 240

Query: 241 KRQKK 243
           K+QKK
Sbjct: 241 KKQKK 245

BLAST of MS021276 vs. NCBI nr
Match: XP_022981239.1 (LOW QUALITY PROTEIN: mediator-associated protein 2-like [Cucurbita maxima])

HSP 1 Score: 327.4 bits (838), Expect = 1.0e-85
Identity = 192/247 (77.73%), Postives = 208/247 (84.21%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MSN+ GA K  E YKP PEFEEDDRE L DISLSDS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSNKVGAVKSVEGYKPPPEFEEDDREFLTDISLSDSVELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
              DGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDT+LVGKI+RRISLVHYPEPEE
Sbjct: 61  HRPDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTRLVGKIARRISLVHYPEPEE 120

Query: 121 LEKATAMKSAFQKSSGISLTTSSR-QTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKAT MKS ++KSSG+SLTTSSR  TTSR PHSSSRRTS   SSKPRSS+SE  EPSKP
Sbjct: 121 LEKATPMKSVYKKSSGMSLTTSSRPTTTSRGPHSSSRRTSSTRSSKPRSSISECGEPSKP 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPS-RSTDRSTQNLGRSHSAVTFSGASGQSSEG 240
           SS K+EPEPSES+D+LKKDSSKRK  H+PS R T+ STQ+ G    A TFSG S  SSE 
Sbjct: 181 SSIKQEPEPSESRDYLKKDSSKRKRXHKPSDRPTNHSTQDEGCDPCAATFSGPSRLSSEC 240

Query: 241 KTKRQKK 243
           KTK++K+
Sbjct: 241 KTKKKKQ 247

BLAST of MS021276 vs. NCBI nr
Match: XP_008463699.1 (PREDICTED: mediator-associated protein 2 [Cucumis melo] >KAA0066809.1 mediator-associated protein 2 [Cucumis melo var. makuwa] >TYK27956.1 mediator-associated protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 326.2 bits (835), Expect = 2.3e-85
Identity = 187/246 (76.02%), Postives = 209/246 (84.96%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MS+E  A K  E YKP  EFEED RE L DISL+DS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSSEVDALKNVEGYKPPAEFEEDRREFLTDISLTDSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DGH+GSFQGSSGKSYDV+SCVAQEPEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGHMGSFQGSSGKSYDVISCVAQEPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKA-TAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKA T++KS  QKSSGISLT S + TTSR P SSSRRT+   SSKPRSSVSE+EEP KP
Sbjct: 121 LEKATTSLKSLCQKSSGISLTNSRQTTTSRGPQSSSRRTTSSRSSKPRSSVSEFEEPWKP 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGK 240
           SS K+E + +ES+DH KKDSSKRK +HEPSRS D ST++ G SHSAVT SG++ +SSEGK
Sbjct: 181 SSVKQELKSTESRDHQKKDSSKRKRSHEPSRSIDHSTRDSGHSHSAVTSSGSAERSSEGK 240

Query: 241 TKRQKK 243
           +K+QKK
Sbjct: 241 SKKQKK 246

BLAST of MS021276 vs. NCBI nr
Match: XP_004146046.1 (mediator-associated protein 2 [Cucumis sativus] >XP_031740697.1 mediator-associated protein 2 [Cucumis sativus] >KGN55103.1 hypothetical protein Csa_012926 [Cucumis sativus])

HSP 1 Score: 323.6 bits (828), Expect = 1.5e-84
Identity = 185/246 (75.20%), Postives = 207/246 (84.15%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MS+E  A K  E YKP  EFEED RE L DISL+DS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSSEVDALKNVEGYKPPAEFEEDKREFLTDISLTDSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DGH+GSFQGSSGKSYDV+SCVAQEPEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGHMGSFQGSSGKSYDVISCVAQEPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKAT-AMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKAT  +KS +QKSSGISLT S + TTSR PHSSSRRT+   SSKPRSSVSE+ EPSK 
Sbjct: 121 LEKATIPLKSLYQKSSGISLTNSRQTTTSRGPHSSSRRTTSSRSSKPRSSVSEFTEPSKT 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGK 240
           SS K+E E +ES+DH +KDSSKRK  HEPSRS D ST++ G  +SAVT SG++ +SSEGK
Sbjct: 181 SSVKQELESTESRDHKRKDSSKRKRLHEPSRSIDHSTRDSGHGNSAVTSSGSAERSSEGK 240

Query: 241 TKRQKK 243
           +K+QKK
Sbjct: 241 SKKQKK 246

BLAST of MS021276 vs. ExPASy Swiss-Prot
Match: F4KF27 (Mediator-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g64680 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 66/205 (32.20%), Postives = 105/205 (51.22%), Query Frame = 0

Query: 13  EYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQLHHDGHLGSFQG 72
           ++K + +F     E L D  L+ S E WLIQ P  Q P+ +     ++   DG  G F+ 
Sbjct: 4   DFKVSGDFIVSGAEQLDDTDLTRSDEFWLIQAPLGQFPEIEENTLKIEPDKDGLFGEFKD 63

Query: 73  SS--------GKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEELEKA 132
           S+        G  YD+ S  +Q+  A + +P S ++ +VGKI+RR++LV YPEP EL   
Sbjct: 64  SNAGSLHLCLGAKYDLASFHSQDAGAELIIP-SEESMIVGKITRRVALVRYPEPNEL--L 123

Query: 133 TAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRR-TSSSKPRSSVSEYEEPSKPSSRKREP 192
             MK+  Q+    S+T SS+++++ T  S  +  T SS+ +S  S + E   P S KR+ 
Sbjct: 124 QKMKARTQQKLVGSVTNSSKKSSNLTQSSRHKSGTRSSREKSMFSGFTE--TPKSPKRKN 183

Query: 193 EPSESKDHLKKDSSKRKHAHEPSRS 209
             S S  H    S+    +   ++S
Sbjct: 184 SESSSGKHRSSTSTVSGSSERSAKS 203

BLAST of MS021276 vs. ExPASy TrEMBL
Match: A0A6J1CDR9 (mediator-associated protein 2 OS=Momordica charantia OX=3673 GN=LOC111010319 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.4e-120
Identity = 238/239 (99.58%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ
Sbjct: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE
Sbjct: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120

Query: 121 LEKATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK 180
           LE+ATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK
Sbjct: 121 LEQATAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTSSSKPRSSVSEYEEPSKPSSRK 180

Query: 181 REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR 240
           REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR
Sbjct: 181 REPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGKTKR 239

BLAST of MS021276 vs. ExPASy TrEMBL
Match: A0A6J1J1C8 (LOW QUALITY PROTEIN: mediator-associated protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480436 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 5.0e-86
Identity = 192/247 (77.73%), Postives = 208/247 (84.21%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MSN+ GA K  E YKP PEFEEDDRE L DISLSDS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSNKVGAVKSVEGYKPPPEFEEDDREFLTDISLSDSVELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
              DGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDT+LVGKI+RRISLVHYPEPEE
Sbjct: 61  HRPDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTRLVGKIARRISLVHYPEPEE 120

Query: 121 LEKATAMKSAFQKSSGISLTTSSR-QTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKAT MKS ++KSSG+SLTTSSR  TTSR PHSSSRRTS   SSKPRSS+SE  EPSKP
Sbjct: 121 LEKATPMKSVYKKSSGMSLTTSSRPTTTSRGPHSSSRRTSSTRSSKPRSSISECGEPSKP 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPS-RSTDRSTQNLGRSHSAVTFSGASGQSSEG 240
           SS K+EPEPSES+D+LKKDSSKRK  H+PS R T+ STQ+ G    A TFSG S  SSE 
Sbjct: 181 SSIKQEPEPSESRDYLKKDSSKRKRXHKPSDRPTNHSTQDEGCDPCAATFSGPSRLSSEC 240

Query: 241 KTKRQKK 243
           KTK++K+
Sbjct: 241 KTKKKKQ 247

BLAST of MS021276 vs. ExPASy TrEMBL
Match: A0A5A7VNG7 (Mediator-associated protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001600 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.1e-85
Identity = 187/246 (76.02%), Postives = 209/246 (84.96%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MS+E  A K  E YKP  EFEED RE L DISL+DS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSSEVDALKNVEGYKPPAEFEEDRREFLTDISLTDSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DGH+GSFQGSSGKSYDV+SCVAQEPEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGHMGSFQGSSGKSYDVISCVAQEPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKA-TAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKA T++KS  QKSSGISLT S + TTSR P SSSRRT+   SSKPRSSVSE+EEP KP
Sbjct: 121 LEKATTSLKSLCQKSSGISLTNSRQTTTSRGPQSSSRRTTSSRSSKPRSSVSEFEEPWKP 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGK 240
           SS K+E + +ES+DH KKDSSKRK +HEPSRS D ST++ G SHSAVT SG++ +SSEGK
Sbjct: 181 SSVKQELKSTESRDHQKKDSSKRKRSHEPSRSIDHSTRDSGHSHSAVTSSGSAERSSEGK 240

Query: 241 TKRQKK 243
           +K+QKK
Sbjct: 241 SKKQKK 246

BLAST of MS021276 vs. ExPASy TrEMBL
Match: A0A1S3CLF4 (mediator-associated protein 2 OS=Cucumis melo OX=3656 GN=LOC103501782 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.1e-85
Identity = 187/246 (76.02%), Postives = 209/246 (84.96%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MS+E  A K  E YKP  EFEED RE L DISL+DS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSSEVDALKNVEGYKPPAEFEEDRREFLTDISLTDSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DGH+GSFQGSSGKSYDV+SCVAQEPEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGHMGSFQGSSGKSYDVISCVAQEPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKA-TAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKA T++KS  QKSSGISLT S + TTSR P SSSRRT+   SSKPRSSVSE+EEP KP
Sbjct: 121 LEKATTSLKSLCQKSSGISLTNSRQTTTSRGPQSSSRRTTSSRSSKPRSSVSEFEEPWKP 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGK 240
           SS K+E + +ES+DH KKDSSKRK +HEPSRS D ST++ G SHSAVT SG++ +SSEGK
Sbjct: 181 SSVKQELKSTESRDHQKKDSSKRKRSHEPSRSIDHSTRDSGHSHSAVTSSGSAERSSEGK 240

Query: 241 TKRQKK 243
           +K+QKK
Sbjct: 241 SKKQKK 246

BLAST of MS021276 vs. ExPASy TrEMBL
Match: A0A0A0L047 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629490 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 7.3e-85
Identity = 185/246 (75.20%), Postives = 207/246 (84.15%), Query Frame = 0

Query: 1   MSNEFGAFKRAEEYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQ 60
           MS+E  A K  E YKP  EFEED RE L DISL+DS ELWLIQWPANQ PDFDGQEFSLQ
Sbjct: 1   MSSEVDALKNVEGYKPPAEFEEDKREFLTDISLTDSEELWLIQWPANQAPDFDGQEFSLQ 60

Query: 61  LHHDGHLGSFQGSSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEE 120
           LH DGH+GSFQGSSGKSYDV+SCVAQEPEASVFLPSSSDT+LVGKISRRISLVHYPEPEE
Sbjct: 61  LHPDGHMGSFQGSSGKSYDVISCVAQEPEASVFLPSSSDTRLVGKISRRISLVHYPEPEE 120

Query: 121 LEKAT-AMKSAFQKSSGISLTTSSRQTTSRTPHSSSRRTS---SSKPRSSVSEYEEPSKP 180
           LEKAT  +KS +QKSSGISLT S + TTSR PHSSSRRT+   SSKPRSSVSE+ EPSK 
Sbjct: 121 LEKATIPLKSLYQKSSGISLTNSRQTTTSRGPHSSSRRTTSSRSSKPRSSVSEFTEPSKT 180

Query: 181 SSRKREPEPSESKDHLKKDSSKRKHAHEPSRSTDRSTQNLGRSHSAVTFSGASGQSSEGK 240
           SS K+E E +ES+DH +KDSSKRK  HEPSRS D ST++ G  +SAVT SG++ +SSEGK
Sbjct: 181 SSVKQELESTESRDHKRKDSSKRKRLHEPSRSIDHSTRDSGHGNSAVTSSGSAERSSEGK 240

Query: 241 TKRQKK 243
           +K+QKK
Sbjct: 241 SKKQKK 246

BLAST of MS021276 vs. TAIR 10
Match: AT5G64680.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; Has 114 Blast hits to 110 proteins in 37 species: Archae - 0; Bacteria - 0; Metazoa - 42; Fungi - 10; Plants - 37; Viruses - 0; Other Eukaryotes - 25 (source: NCBI BLink). )

HSP 1 Score: 75.1 bits (183), Expect = 8.7e-14
Identity = 66/197 (33.50%), Postives = 105/197 (53.30%), Query Frame = 0

Query: 13  EYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQLHHDGHLGSFQG 72
           ++K + +F     E L D  L+ S E WLIQ P  Q P+ +     ++   DG  G F+ 
Sbjct: 4   DFKVSGDFIVSGAEQLDDTDLTRSDEFWLIQAPLGQFPEIEENTLKIEPDKDGLFGEFKD 63

Query: 73  SSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEELEKATAMKSAFQ 132
           S+G  YD+ S  +Q+  A + +P S ++ +VGKI+RR++LV YPEP EL     MK+  Q
Sbjct: 64  SNGAKYDLASFHSQDAGAELIIP-SEESMIVGKITRRVALVRYPEPNEL--LQKMKARTQ 123

Query: 133 KSSGISLTTSSRQTTSRTPHSSSRR-TSSSKPRSSVSEYEEPSKPSSRKREPEPSESKDH 192
           +    S+T SS+++++ T  S  +  T SS+ +S  S + E   P S KR+   S S  H
Sbjct: 124 QKLVGSVTNSSKKSSNLTQSSRHKSGTRSSREKSMFSGFTE--TPKSPKRKNSESSSGKH 183

Query: 193 LKKDSSKRKHAHEPSRS 209
               S+    +   ++S
Sbjct: 184 RSSTSTVSGSSERSAKS 195

BLAST of MS021276 vs. TAIR 10
Match: AT5G64680.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 75.1 bits (183), Expect = 8.7e-14
Identity = 66/197 (33.50%), Postives = 105/197 (53.30%), Query Frame = 0

Query: 13  EYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQLHHDGHLGSFQG 72
           ++K + +F     E L D  L+ S E WLIQ P  Q P+ +     ++   DG  G F+ 
Sbjct: 4   DFKVSGDFIVSGAEQLDDTDLTRSDEFWLIQAPLGQFPEIEENTLKIEPDKDGLFGEFKD 63

Query: 73  SSGKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEELEKATAMKSAFQ 132
           S+G  YD+ S  +Q+  A + +P S ++ +VGKI+RR++LV YPEP EL     MK+  Q
Sbjct: 64  SNGAKYDLASFHSQDAGAELIIP-SEESMIVGKITRRVALVRYPEPNEL--LQKMKARTQ 123

Query: 133 KSSGISLTTSSRQTTSRTPHSSSRR-TSSSKPRSSVSEYEEPSKPSSRKREPEPSESKDH 192
           +    S+T SS+++++ T  S  +  T SS+ +S  S + E   P S KR+   S S  H
Sbjct: 124 QKLVGSVTNSSKKSSNLTQSSRHKSGTRSSREKSMFSGFTE--TPKSPKRKNSESSSGKH 183

Query: 193 LKKDSSKRKHAHEPSRS 209
               S+    +   ++S
Sbjct: 184 RSSTSTVSGSSERSAKS 195

BLAST of MS021276 vs. TAIR 10
Match: AT5G64680.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 53 Blast hits to 53 proteins in 20 species: Archae - 0; Bacteria - 8; Metazoa - 8; Fungi - 0; Plants - 34; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-11
Identity = 66/205 (32.20%), Postives = 105/205 (51.22%), Query Frame = 0

Query: 13  EYKPAPEFEEDDRELLADISLSDSTELWLIQWPANQPPDFDGQEFSLQLHHDGHLGSFQG 72
           ++K + +F     E L D  L+ S E WLIQ P  Q P+ +     ++   DG  G F+ 
Sbjct: 4   DFKVSGDFIVSGAEQLDDTDLTRSDEFWLIQAPLGQFPEIEENTLKIEPDKDGLFGEFKD 63

Query: 73  SS--------GKSYDVVSCVAQEPEASVFLPSSSDTKLVGKISRRISLVHYPEPEELEKA 132
           S+        G  YD+ S  +Q+  A + +P S ++ +VGKI+RR++LV YPEP EL   
Sbjct: 64  SNAGSLHLCLGAKYDLASFHSQDAGAELIIP-SEESMIVGKITRRVALVRYPEPNEL--L 123

Query: 133 TAMKSAFQKSSGISLTTSSRQTTSRTPHSSSRR-TSSSKPRSSVSEYEEPSKPSSRKREP 192
             MK+  Q+    S+T SS+++++ T  S  +  T SS+ +S  S + E   P S KR+ 
Sbjct: 124 QKMKARTQQKLVGSVTNSSKKSSNLTQSSRHKSGTRSSREKSMFSGFTE--TPKSPKRKN 183

Query: 193 EPSESKDHLKKDSSKRKHAHEPSRS 209
             S S  H    S+    +   ++S
Sbjct: 184 SESSSGKHRSSTSTVSGSSERSAKS 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139366.12.9e-12099.58mediator-associated protein 2 [Momordica charantia] >XP_022139367.1 mediator-ass... [more]
XP_038896123.12.5e-8777.14mediator-associated protein 2 [Benincasa hispida][more]
XP_022981239.11.0e-8577.73LOW QUALITY PROTEIN: mediator-associated protein 2-like [Cucurbita maxima][more]
XP_008463699.12.3e-8576.02PREDICTED: mediator-associated protein 2 [Cucumis melo] >KAA0066809.1 mediator-a... [more]
XP_004146046.11.5e-8475.20mediator-associated protein 2 [Cucumis sativus] >XP_031740697.1 mediator-associa... [more]
Match NameE-valueIdentityDescription
F4KF271.9e-1032.20Mediator-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g64680 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CDR91.4e-12099.58mediator-associated protein 2 OS=Momordica charantia OX=3673 GN=LOC111010319 PE=... [more]
A0A6J1J1C85.0e-8677.73LOW QUALITY PROTEIN: mediator-associated protein 2-like OS=Cucurbita maxima OX=3... [more]
A0A5A7VNG71.1e-8576.02Mediator-associated protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CLF41.1e-8576.02mediator-associated protein 2 OS=Cucumis melo OX=3656 GN=LOC103501782 PE=4 SV=1[more]
A0A0A0L0477.3e-8575.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629490 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G64680.18.7e-1433.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G64680.28.7e-1433.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G64680.31.4e-1132.20unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..208
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
IPR038823Mediator-associated protein 2, plantPANTHERPTHR36407MEDIATOR-ASSOCIATED PROTEIN 2coord: 9..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021276.1MS021276.1mRNA