MS021273 (gene) Bitter gourd (TR) v1

Overview


Name	MS021273
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	D-amino-acid transaminase, chloroplastic-like
Location	scaffold358: 643814 .. 644974 (-)
RNA-Seq Expression	MS021273
Synteny	MS021273

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTTGCTTGAAAAACTTCATGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCAATGTATTCCAGCATTTTTGGTGGAATCACAACAGACCCAGCTCTGATGGTGATTCCTATTGATGATCACATGGTTCATCGAGGCCACGGCGTGTTCGATACCGCCGTTCTTTTAAATGGGTAGGTACATGGGGATCTCTCGTCAACTTTAGTTCAGTTTCTCGAAAATTCTGAAGATGCTAAACAATTCTATTTTAACACAACTTTCCTTGGATAATGGTCGTTGTTTTGGCGTCTAACAGACATTTGTATGAATTGGATGCCCACCTCAACCGCTTCCTAAGATCAGCTTCAAAAGCAAAGATCTCCCCTCCCTTTCCTTGGTCGATTCTTCGGAGCATTCTTATCCAATTGGCAGTAGCATCACAGCTCAAGAAAGGGACTCTAAGATACTGGCTAAGTGCAGGACCTGGAAATTTTTTGCTCACTCCTGCTGGAAATGCCAATTCTACATTTTATGCGGTAGCCATCGACGAAGACTTCTCTCAATGCAAAGAAGGGGTTAAAGTGATAACATCTACCATCCCAATGAAGACACCTCAGTTTGCAACGATGAAGAATGTGAACTACCTACCGAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCTATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCTTTCATAACCAGTGAAAAGGAGCTTATCTTGCCTTCTTTTGATAAGATCCTCTCGGGCTGCACCGCCCTCCGGCTTCTAAAGCTAGCTCCAAAGTTGGTTGAGGAGGGGAAGCTGAAGAGTGTAGGAACTGCAAACGTTACGGTGAAGGAAGCCAAAGGTGCTGCTGAAATGATGTTCGTAGGAAGCACGCTTCCGATACTGCCAATCATCACCTGGGATGAAGAACCCATTGGAGATGGTAACAACTTTTTTCTGACATAAACTTTTGTTGTTAAAGTGATAGTGAATGAAATGTTCTTATAGGAGATTCCATTGATCCTCTGTAACAGGAAGGGTTGGAGAATTGACAATGGCGCTATCTGATCTGCTTTGGGATGACATGGTTTTGGGCCCCGAAACGGAGAGAGTACCTGTTCCATACATG

mRNA sequence

CAGTTGCTTGAAAAACTTCATGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCAATGTATTCCAGCATTTTTGGTGGAATCACAACAGACCCAGCTCTGATGGTGATTCCTATTGATGATCACATGGTTCATCGAGGCCACGGCGTGTTCGATACCGCCGTTCTTTTAAATGGACATTTGTATGAATTGGATGCCCACCTCAACCGCTTCCTAAGATCAGCTTCAAAAGCAAAGATCTCCCCTCCCTTTCCTTGGTCGATTCTTCGGAGCATTCTTATCCAATTGGCAGTAGCATCACAGCTCAAGAAAGGGACTCTAAGATACTGGCTAAGTGCAGGACCTGGAAATTTTTTGCTCACTCCTGCTGGAAATGCCAATTCTACATTTTATGCGGTAGCCATCGACGAAGACTTCTCTCAATGCAAAGAAGGGGTTAAAGTGATAACATCTACCATCCCAATGAAGACACCTCAGTTTGCAACGATGAAGAATGTGAACTACCTACCGAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCTATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCTTTCATAACCAGTGAAAAGGAGCTTATCTTGCCTTCTTTTGATAAGATCCTCTCGGGCTGCACCGCCCTCCGGCTTCTAAAGCTAGCTCCAAAGTTGGTTGAGGAGGGGAAGCTGAAGAGTGTAGGAACTGCAAACGTTACGGTGAAGGAAGCCAAAGGTGCTGCTGAAATGATGTTCGTAGGAAGCACGCTTCCGATACTGCCAATCATCACCTGGGATGAAGAACCCATTGGAGATGGAAGGGTTGGAGAATTGACAATGGCGCTATCTGATCTGCTTTGGGATGACATGGTTTTGGGCCCCGAAACGGAGAGAGTACCTGTTCCATACATG

Coding sequence (CDS)

CAGTTGCTTGAAAAACTTCATGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCAATGTATTCCAGCATTTTTGGTGGAATCACAACAGACCCAGCTCTGATGGTGATTCCTATTGATGATCACATGGTTCATCGAGGCCACGGCGTGTTCGATACCGCCGTTCTTTTAAATGGACATTTGTATGAATTGGATGCCCACCTCAACCGCTTCCTAAGATCAGCTTCAAAAGCAAAGATCTCCCCTCCCTTTCCTTGGTCGATTCTTCGGAGCATTCTTATCCAATTGGCAGTAGCATCACAGCTCAAGAAAGGGACTCTAAGATACTGGCTAAGTGCAGGACCTGGAAATTTTTTGCTCACTCCTGCTGGAAATGCCAATTCTACATTTTATGCGGTAGCCATCGACGAAGACTTCTCTCAATGCAAAGAAGGGGTTAAAGTGATAACATCTACCATCCCAATGAAGACACCTCAGTTTGCAACGATGAAGAATGTGAACTACCTACCGAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCTATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCTTTCATAACCAGTGAAAAGGAGCTTATCTTGCCTTCTTTTGATAAGATCCTCTCGGGCTGCACCGCCCTCCGGCTTCTAAAGCTAGCTCCAAAGTTGGTTGAGGAGGGGAAGCTGAAGAGTGTAGGAACTGCAAACGTTACGGTGAAGGAAGCCAAAGGTGCTGCTGAAATGATGTTCGTAGGAAGCACGCTTCCGATACTGCCAATCATCACCTGGGATGAAGAACCCATTGGAGATGGAAGGGTTGGAGAATTGACAATGGCGCTATCTGATCTGCTTTGGGATGACATGGTTTTGGGCCCCGAAACGGAGAGAGTACCTGTTCCATACATG

Protein sequence

QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWDDMVLGPETERVPVPYM

Homology

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BLAST of MS021273 vs. NCBI nr
Match: XP_022139744.1 (D-amino-acid transaminase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 627.1 bits (1616), Expect = 8.3e-176
Identity = 310/316 (98.10%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG
Sbjct: 39  ELLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 98

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           HLYELD HLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL
Sbjct: 99  HLYELDGHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 158

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE
Sbjct: 159 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 218

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLVEE
Sbjct: 219 EKGAFASIWVDEEGYIAEGPNVNVAFITTEKELILPSFDKILSGCTALRLLKLAPKLVEE 278

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGR GELTMALSDLLWD
Sbjct: 279 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRAGELTMALSDLLWD 338

Query: 301 DMVLGPETERVPVPYM 317
           DMV GP+TERVPVPYM
Sbjct: 339 DMVSGPKTERVPVPYM 354

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BLAST of MS021273 vs. NCBI nr
Match: XP_004146044.1 (D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypothetical protein Csa_012285 [Cucumis sativus])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 286/315 (90.79%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV KKPYPAMYSS+FGGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 32  ELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 91

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 92  YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVSQLKKGTLRYWLSAGPGDFL 151

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA NANS FYAVAID+DFSQCKEGVK ITSTIPMKTPQFATMKNVNYLPNVLAK+EAE
Sbjct: 152 LTPAVNANSAFYAVAIDDDFSQCKEGVKAITSTIPMKTPQFATMKNVNYLPNVLAKLEAE 211

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 212 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 271

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAK AAEMMFVGSTLP+LPII+WDEEPIGDGRVGELTMALSDLLWD
Sbjct: 272 GKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSDLLWD 331

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 332 DMVSGPETERIPVSY 346

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BLAST of MS021273 vs. NCBI nr
Match: KAA0066805.1 (hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK27952.1 hypothetical protein E5676_scaffold384G001560 [Cucumis melo var. makuwa])

HSP 1 Score: 582.0 bits (1499), Expect = 3.1e-162
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV+KKPYPAMYSSI GGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 424 ELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 483

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 484 YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVSQLKKGTLRYWLSAGPGDFL 543

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA +ANS FYAVAID+DFSQCKEGVKVIT+TIPMKTP FATMKNVNYLPNVLAKMEAE
Sbjct: 544 LTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLFATMKNVNYLPNVLAKMEAE 603

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 604 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 663

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAKGAAEMMFVGSTLP+LPII+WDEEPIGDG VGELTMALSDLLWD
Sbjct: 664 GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGHVGELTMALSDLLWD 723

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 724 DMVSGPETERIPVSY 738

[Close]

BLAST of MS021273 vs. NCBI nr
Match: XP_008463704.1 (PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 582.0 bits (1499), Expect = 3.1e-162
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV+KKPYPAMYSSI GGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 32  ELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 91

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 92  YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVSQLKKGTLRYWLSAGPGDFL 151

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA +ANS FYAVAID+DFSQCKEGVKVIT+TIPMKTP FATMKNVNYLPNVLAKMEAE
Sbjct: 152 LTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLFATMKNVNYLPNVLAKMEAE 211

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 212 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 271

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAKGAAEMMFVGSTLP+LPII+WDEEPIGDG VGELTMALSDLLWD
Sbjct: 272 GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGHVGELTMALSDLLWD 331

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 332 DMVSGPETERIPVSY 346

[Close]

BLAST of MS021273 vs. NCBI nr
Match: XP_038900251.1 (D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 581.6 bits (1498), Expect = 4.0e-162
Identity = 282/315 (89.52%), Postives = 301/315 (95.56%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLH+KWSLVKKKPYPAMYSSIFGGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 99  ELLEKLHQKWSLVKKKPYPAMYSSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 158

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           HLYELDAHL+RFLRSASKAKIS PFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 159 HLYELDAHLDRFLRSASKAKISSPFPRSILRSILIQLTAVSQLKKGTLRYWLSAGPGDFL 218

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA NA S FYAVAID+DFSQCKEGVK IT+T+PMKTPQFATMKNVNYLPNVLAK+EAE
Sbjct: 219 LTPAVNAKSAFYAVAIDDDFSQCKEGVKAITTTVPMKTPQFATMKNVNYLPNVLAKLEAE 278

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELI PSFDK+LSGCTALRLLK+APKLVE+
Sbjct: 279 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELIFPSFDKVLSGCTALRLLKIAPKLVED 338

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAKGAAEMMFVGSTLP+LPII+WD EPIGDG VGELTMALSDLLWD
Sbjct: 339 GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDGEPIGDGSVGELTMALSDLLWD 398

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PVPY
Sbjct: 399 DMVSGPETERIPVPY 413

[Close]

BLAST of MS021273 vs. ExPASy Swiss-Prot
Match: Q8L493 (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 7.2e-98
Identity = 177/297 (59.60%), Postives = 227/297 (76.43%), Query Frame = 0


Query: 20  AMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKA 79
           AMYSS+  GITTDPA MV+P+DDHMVHRGHGVFDTA+++NG+LYELD HL+R LRSAS A
Sbjct: 77  AMYSSVVDGITTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMA 136

Query: 80  KISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDED 139
           KI  PF    ++ ILIQ    S  + G+LRYWLSAGPG+FLL+P+     T YA+ I  +
Sbjct: 137 KIPLPFDRETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTN 196

Query: 140 FSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEG 199
           F+    GVKV+TS+IP+K P+FAT+K+VNYLPNVL++MEAE KGA+A IWV ++G+IAEG
Sbjct: 197 FAINPIGVKVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEG 256

Query: 200 PNVNVAFITS-EKELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKG 259
           PN+NVAF+ +  KEL++P FD +LSGCTA R L LA +LV +G LK+V   +VTV++ K 
Sbjct: 257 PNMNVAFVVNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKK 316

Query: 260 AAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWDDMVLGPETERVPVPY 316
           A EMM +GS +PI P+I WDEE IG+G+ G +  AL DLL +DM  GP + RV VPY
Sbjct: 317 ADEMMLIGSGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of MS021273 vs. ExPASy Swiss-Prot
Match: Q58414 (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=ilvE PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.9e-18
Identity = 69/266 (25.94%), Postives = 127/266 (47.74%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQL 97
           + + DH +  G GVF+     +G ++ L  H++R   SA    I  P     +  ++++ 
Sbjct: 17  VSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLET 76

Query: 98  AVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTI--- 157
              + L+   +R  ++ G G+  L P      T + +AI       ++G++ IT ++   
Sbjct: 77  LRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVRRL 136

Query: 158 PMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEKELI 217
           P+     A +K++NYL +VLAK++A   G   +  +D++G++ EG   N+  + +     
Sbjct: 137 PVDVLNPA-VKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKT 196

Query: 218 LPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPI 277
            P +  IL G T   ++KLA    EEG    V    +T+ +   A E+   G+   I+P+
Sbjct: 197 PPVYQSILKGITRDVVIKLAK---EEG--IEVVEEPLTLHDLYTADELFITGTAAEIVPV 256

Query: 278 ITWDEEPIGDGRVGELTMALSDLLWD 301
              D   I + +VGE+T  L +   D
Sbjct: 257 FEIDGRVINNKQVGEITKKLKEKFKD 276

[Close]

BLAST of MS021273 vs. ExPASy Swiss-Prot
Match: O29329 (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=ilvE PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.5e-18
Identity = 73/283 (25.80%), Postives = 130/283 (45.94%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQL 97
           + I DH    G GVF+     NG ++ L  H++R   SA    +  P        I+++ 
Sbjct: 18  VSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPITKEEFMEIILET 77

Query: 98  AVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDEDFSQC-----KEGVKVITS 157
              + L+   +R  ++ G G+  L P    N +   + I + + +      ++G+  IT 
Sbjct: 78  LRKNNLRDAYIRPIVTRGIGDLGLDPRKCQNPSI--IVITKPWGKLYGDLYEKGLTAITV 137

Query: 158 TIPMKTPQF--ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSE 217
            +   +       +K++NYL N+LAK+EA  KG   +I++D  GY++EG   N+ F+   
Sbjct: 138 AVRRNSFDALPPNIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI-FVVKN 197

Query: 218 KELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKGAAEMMFVGSTLP 277
             +  P     L G T   ++++  +L    K  ++G  ++       A E+   G+   
Sbjct: 198 GAITTPPTINNLRGITREAVIEIINRLGIPFKETNIGLYDLYT-----ADEVFVTGTAAE 257

Query: 278 ILPIITWDEEPIGDGRVGELTMALSDLLWDDMVLGPETERVPV 314
           I PI+  D   IGDG+ GE+T      L ++     E+E VP+
Sbjct: 258 IAPIVVIDGRKIGDGKPGEITRK----LMEEFSKLTESEGVPI 288

[Close]

BLAST of MS021273 vs. ExPASy Swiss-Prot
Match: O07597 (D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.1e-16
Identity = 71/259 (27.41%), Postives = 122/259 (47.10%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQL 97
           I ++D     G G+++   +  G L+ L  H  RF RSA++  IS PF    L   L +L
Sbjct: 17  IDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKL 76

Query: 98  AVASQLKKGTLRYWLSAG--PGNFLLTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIP 157
              + + +G +    + G  P            +T Y   + +   +   GV  IT    
Sbjct: 77  VQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDE-D 136

Query: 158 MKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEKELIL 217
           ++  +   +K++N L NV+ K  A E GAF +I +  +G + EG + NV  + +      
Sbjct: 137 LRWLR-CDIKSLNLLYNVMTKQRAYEAGAFEAILL-RDGVVTEGTSSNVYAVINGTVRTH 196

Query: 218 PSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPII 277
           P+   IL+G T + +L     L+E+  +K +    V+ +E K A E+    +T  I+P++
Sbjct: 197 PANRLILNGITRMNIL----GLIEKNGIK-LDETPVSEEELKQAEEIFISSTTAEIIPVV 256

Query: 278 TWDEEPIGDGRVGELTMAL 295
           T D + IG G+ G +T  L
Sbjct: 257 TLDGQSIGSGKPGPVTKQL 267

[Close]

BLAST of MS021273 vs. ExPASy Swiss-Prot
Match: P63511 (D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=dat PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 69/279 (24.73%), Postives = 118/279 (42.29%), Query Frame = 0


Query: 21  MYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKAK 80
           M      G    P+   +  +D     G G+++   + NG L+ +  H  RFLRSA++  
Sbjct: 1   MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIG 60

Query: 81  ISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAG---PGNFLLTPAGNANSTFYAVAID 140
           +   +    L  +  +L   +Q++ G +    + G     +   TP        Y  + D
Sbjct: 61  LDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYD 120

Query: 141 EDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIA 200
             +   + GV  +  T+         +K++N L NVLAK  A +  A  +I    E  + 
Sbjct: 121 RPYDHLENGVNGV--TVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGE-TVT 180

Query: 201 EGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAK 260
           EG + N   I        P  + IL+G T + + K+A       K ++      TV   K
Sbjct: 181 EGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEET-----FTVDFLK 240

Query: 261 GAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSD 297
            A E++   ++  + P+I  D EPI DG+VG +T  L +
Sbjct: 241 NADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQE 271

[Close]

BLAST of MS021273 vs. ExPASy TrEMBL
Match: A0A6J1CDV8 (D-amino-acid transaminase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010583 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.0e-176
Identity = 310/316 (98.10%), Postives = 313/316 (99.05%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG
Sbjct: 39  ELLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 98

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           HLYELD HLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL
Sbjct: 99  HLYELDGHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 158

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE
Sbjct: 159 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 218

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLVEE
Sbjct: 219 EKGAFASIWVDEEGYIAEGPNVNVAFITTEKELILPSFDKILSGCTALRLLKLAPKLVEE 278

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGR GELTMALSDLLWD
Sbjct: 279 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRAGELTMALSDLLWD 338

Query: 301 DMVLGPETERVPVPYM 317
           DMV GP+TERVPVPYM
Sbjct: 339 DMVSGPKTERVPVPYM 354

[Close]

BLAST of MS021273 vs. ExPASy TrEMBL
Match: A0A0A0KZK6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.0e-163
Identity = 286/315 (90.79%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV KKPYPAMYSS+FGGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 32  ELLEKLHEKWSLVNKKPYPAMYSSVFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 91

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 92  YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTAVSQLKKGTLRYWLSAGPGDFL 151

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA NANS FYAVAID+DFSQCKEGVK ITSTIPMKTPQFATMKNVNYLPNVLAK+EAE
Sbjct: 152 LTPAVNANSAFYAVAIDDDFSQCKEGVKAITSTIPMKTPQFATMKNVNYLPNVLAKLEAE 211

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 212 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 271

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAK AAEMMFVGSTLP+LPII+WDEEPIGDGRVGELTMALSDLLWD
Sbjct: 272 GKLKSVGTANLTVKEAKDAAEMMFVGSTLPLLPIISWDEEPIGDGRVGELTMALSDLLWD 331

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 332 DMVSGPETERIPVSY 346

[Close]

BLAST of MS021273 vs. ExPASy TrEMBL
Match: A0A5D3DWQ3 (Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001560 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV+KKPYPAMYSSI GGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 424 ELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 483

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 484 YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVSQLKKGTLRYWLSAGPGDFL 543

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA +ANS FYAVAID+DFSQCKEGVKVIT+TIPMKTP FATMKNVNYLPNVLAKMEAE
Sbjct: 544 LTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLFATMKNVNYLPNVLAKMEAE 603

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 604 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 663

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAKGAAEMMFVGSTLP+LPII+WDEEPIGDG VGELTMALSDLLWD
Sbjct: 664 GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGHVGELTMALSDLLWD 723

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 724 DMVSGPETERIPVSY 738

[Close]

BLAST of MS021273 vs. ExPASy TrEMBL
Match: A0A1S3CJV5 (D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103501786 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSLV+KKPYPAMYSSI GGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNG
Sbjct: 32  ELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNG 91

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           +LYELDAH++RFLRSASKAKISPPFP SILRSILIQL   SQLKKGTLRYWLSAGPG+FL
Sbjct: 92  YLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVSQLKKGTLRYWLSAGPGDFL 151

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTPA +ANS FYAVAID+DFSQCKEGVKVIT+TIPMKTP FATMKNVNYLPNVLAKMEAE
Sbjct: 152 LTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLFATMKNVNYLPNVLAKMEAE 211

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVDEEGYIAEGPNVNVAFIT+EKELILPSFDKILSGCTALRLLKLAPKLV+E
Sbjct: 212 EKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKILSGCTALRLLKLAPKLVKE 271

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
           GKLKSVGTAN+TVKEAKGAAEMMFVGSTLP+LPII+WDEEPIGDG VGELTMALSDLLWD
Sbjct: 272 GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGHVGELTMALSDLLWD 331

Query: 301 DMVLGPETERVPVPY 316
           DMV GPETER+PV Y
Sbjct: 332 DMVSGPETERIPVSY 346

[Close]

BLAST of MS021273 vs. ExPASy TrEMBL
Match: A0A6J1FBG0 (D-amino-acid transaminase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442546 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.3e-155
Identity = 269/315 (85.40%), Postives = 296/315 (93.97%), Query Frame = 0


Query: 1   QLLEKLHEKWSLVKKKPYPAMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNG 60
           +LLEKLHEKWSL KKKPYPAMYSSIFGGI TDPA+M+IPIDDHMVHRGHGVFDTA++LNG
Sbjct: 32  ELLEKLHEKWSL-KKKPYPAMYSSIFGGIITDPAMMMIPIDDHMVHRGHGVFDTAIILNG 91

Query: 61  HLYELDAHLNRFLRSASKAKISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFL 120
           HLYELDAH++RFLRSA+KAKIS PFP S+LRSILIQL   S+LKKGTLRYWLSAGPG+FL
Sbjct: 92  HLYELDAHIDRFLRSAAKAKISSPFPRSVLRSILIQLTAVSELKKGTLRYWLSAGPGDFL 151

Query: 121 LTPAGNANSTFYAVAIDEDFSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAE 180
           LTP+G+ NS FYAVAID+DFSQCKEGVK ITST+P+KTPQFATMK+VNYLPNVL KMEAE
Sbjct: 152 LTPSGHGNSAFYAVAIDDDFSQCKEGVKAITSTVPIKTPQFATMKSVNYLPNVLTKMEAE 211

Query: 181 EKGAFASIWVDEEGYIAEGPNVNVAFITSEKELILPSFDKILSGCTALRLLKLAPKLVEE 240
           EKGAFASIWVD+EGY+AEGPNVNVAFIT EKELILP FDK+LSGCTALRLL LAPKLVE+
Sbjct: 212 EKGAFASIWVDDEGYVAEGPNVNVAFITKEKELILPCFDKVLSGCTALRLLALAPKLVED 271

Query: 241 GKLKSVGTANVTVKEAKGAAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWD 300
            KLKSVGTAN+TV+EAK AAEMMFVGSTLP+LPII+WD+EPIGDGRVGELTMALSDLLWD
Sbjct: 272 RKLKSVGTANLTVEEAKDAAEMMFVGSTLPVLPIISWDDEPIGDGRVGELTMALSDLLWD 331

Query: 301 DMVLGPETERVPVPY 316
           DMV G E ER+PVPY
Sbjct: 332 DMVSGSEIERIPVPY 345

[Close]

BLAST of MS021273 vs. TAIR 10
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 5.1e-99
Identity = 177/297 (59.60%), Postives = 227/297 (76.43%), Query Frame = 0


Query: 20  AMYSSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSASKA 79
           AMYSS+  GITTDPA MV+P+DDHMVHRGHGVFDTA+++NG+LYELD HL+R LRSAS A
Sbjct: 77  AMYSSVVDGITTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMA 136

Query: 80  KISPPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDED 139
           KI  PF    ++ ILIQ    S  + G+LRYWLSAGPG+FLL+P+     T YA+ I  +
Sbjct: 137 KIPLPFDRETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTN 196

Query: 140 FSQCKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEG 199
           F+    GVKV+TS+IP+K P+FAT+K+VNYLPNVL++MEAE KGA+A IWV ++G+IAEG
Sbjct: 197 FAINPIGVKVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEG 256

Query: 200 PNVNVAFITS-EKELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKG 259
           PN+NVAF+ +  KEL++P FD +LSGCTA R L LA +LV +G LK+V   +VTV++ K 
Sbjct: 257 PNMNVAFVVNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKK 316

Query: 260 AAEMMFVGSTLPILPIITWDEEPIGDGRVGELTMALSDLLWDDMVLGPETERVPVPY 316
           A EMM +GS +PI P+I WDEE IG+G+ G +  AL DLL +DM  GP + RV VPY
Sbjct: 317 ADEMMLIGSGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY 373

[Close]

BLAST of MS021273 vs. TAIR 10
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 5.6e-13
Identity = 65/268 (24.25%), Postives = 123/268 (45.90%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSA-SKAKISPPFPWSILRSILIQ 97
           + + D +V  G  V++   +  G +++L+ HL+R   SA + A  + P    +  +I   
Sbjct: 278 VSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPAREEVKEAIFRT 337

Query: 98  LAVASQLKKGTLRYWLSAGPG-NFLLTPAGNANSTFYAVAID--EDFSQCKEGVKVITST 157
           L          +R  L+ G      ++PA N       V  +        + G+ ++T+T
Sbjct: 338 LITNGMFDNTHIRLSLTRGKKVTSGMSPAYNRYGCTLIVLAEWKPPVYDNEGGIVLVTAT 397

Query: 158 IPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEK 217
               +P     K  + N L N+LAK+E+    A  +I +D++GY++E    N+ F+  + 
Sbjct: 398 TRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETNATNI-FMVKKG 457

Query: 218 ELILPSFDKILSGCTALRLLKLAPK---LVEEGKLKSVGTANVTVKEAKGAAEMMFVGST 277
            ++ P  D  L G T   +++L  K   ++EE +        +++ E   A E+   G+ 
Sbjct: 458 CVLTPHADYCLPGITRATVMELVVKENFILEERR--------ISLSEFHTANEVWTTGTM 517

Query: 278 LPILPIITWDEEPIGDGRVGELTMALSD 297
             + P++  D   IGDG+VG +T  L +
Sbjct: 518 GELSPVVKIDGRVIGDGKVGPVTRTLQN 536

[Close]

BLAST of MS021273 vs. TAIR 10
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 63/268 (23.51%), Postives = 121/268 (45.15%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSA-SKAKISPPFPWSILRSILIQ 97
           + + D +V  G  V++   +  G +++L+ HL+R   SA + A  + P    I  +I   
Sbjct: 275 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPTREEIKEAIFRT 334

Query: 98  LAVASQLKKGTLRYWLSAGPG-NFLLTPAGNANSTFYAVAID--EDFSQCKEGVKVITST 157
           L          +R  L+ G      ++PA N       V  +          G+ ++T+T
Sbjct: 335 LITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYDNDGGIVLVTAT 394

Query: 158 IPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEK 217
               +P     K  + N L N+LAK+E+       +I +D++G+++E    N+ F+  + 
Sbjct: 395 TRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATNI-FMVKKD 454

Query: 218 ELILPSFDKILSGCTALRLLKLAPK---LVEEGKLKSVGTANVTVKEAKGAAEMMFVGST 277
            ++ P  D  L G T   +++L  K   ++EE +        +++ E   A E+   G+ 
Sbjct: 455 RVLTPHADYCLPGITRATVMELVVKENFILEERR--------ISLSEFHTADEVWTTGTM 514

Query: 278 LPILPIITWDEEPIGDGRVGELTMALSD 297
             + P++  D   IG+G+VG +T  L +
Sbjct: 515 GELSPVVKIDGRVIGEGKVGPVTRRLQN 533

[Close]

BLAST of MS021273 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-11
Identity = 63/268 (23.51%), Postives = 121/268 (45.15%), Query Frame = 0


Query: 38  IPIDDHMVHRGHGVFDTAVLLNGHLYELDAHLNRFLRSA-SKAKISPPFPWSILRSILIQ 97
           + + D +V  G  V++   +  G +++L+ HL+R   SA + A  + P    I  +I   
Sbjct: 652 VSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPTREEIKEAIFRT 711

Query: 98  LAVASQLKKGTLRYWLSAGPG-NFLLTPAGNANSTFYAVAID--EDFSQCKEGVKVITST 157
           L          +R  L+ G      ++PA N       V  +          G+ ++T+T
Sbjct: 712 LITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYDNDGGIVLVTAT 771

Query: 158 IPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITSEK 217
               +P     K  + N L N+LAK+E+       +I +D++G+++E    N+ F+  + 
Sbjct: 772 TRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETNATNI-FMVKKD 831

Query: 218 ELILPSFDKILSGCTALRLLKLAPK---LVEEGKLKSVGTANVTVKEAKGAAEMMFVGST 277
            ++ P  D  L G T   +++L  K   ++EE +        +++ E   A E+   G+ 
Sbjct: 832 RVLTPHADYCLPGITRATVMELVVKENFILEERR--------ISLSEFHTADEVWTTGTM 891

Query: 278 LPILPIITWDEEPIGDGRVGELTMALSD 297
             + P++  D   IG+G+VG +T  L +
Sbjct: 892 GELSPVVKIDGRVIGEGKVGPVTRRLQN 910

The following BLAST results are available for this feature:

BLAST of MS021273 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139744.1	8.3e-176	98.10	D-amino-acid transaminase, chloroplastic-like [Momordica charantia]	[more]
XP_004146044.1	2.1e-163	90.79	D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypotheti...	[more]
KAA0066805.1	3.1e-162	90.48	hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK279...	[more]
XP_008463704.1	3.1e-162	90.48	PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo]	[more]
XP_038900251.1	4.0e-162	89.52	D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida]	[more]

BLAST of MS021273 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L493	7.2e-98	59.60	D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT...	[more]
Q58414	1.9e-18	25.94	Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannas...	[more]
O29329	2.5e-18	25.80	Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (s...	[more]
O07597	3.1e-16	27.41	D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE...	[more]
P63511	1.0e-11	24.73	D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) ...	[more]

BLAST of MS021273 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDV8	4.0e-176	98.10	D-amino-acid transaminase, chloroplastic-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A0A0KZK6	1.0e-163	90.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1	[more]
A0A5D3DWQ3	1.5e-162	90.48	Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CJV5	1.5e-162	90.48	D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A6J1FBG0	2.3e-155	85.40	D-amino-acid transaminase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of MS021273 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57850.1	5.1e-99	59.60	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT3G05190.1	5.6e-13	24.25	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.1	1.8e-11	23.51	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]
AT5G27410.2	1.8e-11	23.51	D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001544	Aminotransferase class IV	PFAM	PF01063	Aminotran_4	coord: 50..275 e-value: 3.2E-41 score: 141.6
IPR043132	Branched-chain-amino-acid aminotransferase-like, C-terminal	GENE3D	3.20.10.10		coord: 138..304 e-value: 2.7E-36 score: 126.5
IPR043131	Branched-chain-amino-acid aminotransferase-like, N-terminal	GENE3D	3.30.470.10		coord: 4..136 e-value: 1.9E-22 score: 81.8
None	No IPR available	PANTHER	PTHR42743	AMINO-ACID AMINOTRANSFERASE	coord: 2..314
None	No IPR available	PANTHER	PTHR42743:SF8	BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE-LIKE	coord: 2..314
IPR017824	Aminodeoxychorismate lyase, class IV	CDD	cd01559	ADCL_like	coord: 42..298 e-value: 2.65717E-73 score: 224.496
IPR036038	Aminotransferase-like, PLP-dependent enzymes	SUPERFAMILY	56752	D-aminoacid aminotransferase-like PLP-dependent enzymes	coord: 26..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021273.1	MS021273.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046656	folic acid biosynthetic process
molecular_function	GO:0008696	4-amino-4-deoxychorismate lyase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0030170	pyridoxal phosphate binding