MS021234 (gene) Bitter gourd (TR) v1

Overview
NameMS021234
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein transport protein Sec61 subunit alpha-like
Locationscaffold358: 361363 .. 363821 (-)
RNA-Seq ExpressionMS021234
SyntenyMS021234
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGGTGGATTTCGAGTTCTTCACTTGGTCAGACCGTTTCTTTCGTTCCTCCCAGAAGTTCAGAGCGCTGACAGGAAAGTTCCATTCAGAGAGAAGGTTATTTATACAGTGATTTCGCTTTTCATTTTCTTGGTTTGTAGCCAACTACCTCTGTATGGCATACACTCCACAACGGGAGCAGATCCGTTTTATTGGATGCGTGTGATTCTTGCTTCAAATCGTGGCACTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTGATGCAGCTCTTAGTTGGGTCTAAGATCATTGAAGTTGACAACAATGTGAGAGAGGACCGCGCACTCTTGTAATTCTCTGGGCCTTAATCTATAATTTTCAGTTCATAGATTCTTTTCAACGCCCCTCATTCCCATTTTACTTTTAGTTTGTCTCTAAAATTCTTAGTGACTGTGAGCTTTGTTTTTGTTGCTCTCCAAGATAATAATGTAAATTTTATAGTTTATTCTTCATTTGATGATTCTTCTTTTGCTCTATAGAAATGGGGCACAAAAATTGCTCGGTATACTCATAGCAATTGGGGAGGCGGTTGCTTATGTTCTCTCTGGCATGTACGGCAGTGTTGATCAACTTGGAGTGGGCAATGCCATTCTTATTATTGTTCAGCTCTGTTTTGCTGGTATCATCGTCATTTGTTTAGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACCAATATCTGGTGACATTCTTAACTTTGCTTTTGACGTTTCATTTCTATACTGTTTGTTCTTGCAATTTCTATGCTCCTTGCTTCTACCCTGCACATCACCTTGTTTCTAAATTCTAAGCATTAAAAACCTTGAATTGCTCTTTAATTTGCAGTGAGAACATTATTTGGAAAGCATTTAGTCCCACTACAATCAATAGTGGACGAGGAGCTGAATTTGAAGGAGCTGTTATTGCTCTATTTCATCTGCTGATAGCTCGGTCAGACAAAGTTAGAGCACTTCGTGAGGCATTTTACCGACAAAATCTTCCCAATGTCACAAATCTGCTTGCAACAGTTTTTATCTTTTTAATTGTGATCTACTTCCAAGGATTCCGGGTGGTTTTGCCTGTGAGGTCAAAGAATGCCCGCGGACAACAAGGTTCATACCCAATCAAGCTGTTCTACACTTCCAACATGCCAATCATTCTACAATCTGCACTTGTATCAAATGTTTATTTCATCTCTCAGGTAGGTCAACTTTTTGCATTAAATGCAGGACTCCTGGTGAATTAGTGATGCTTTGCCATCTAACTAATCATTTTGTGAATCTGCACAGTTGCTGTACAGGAAGTATGGTGGAAACTTTCTCGTGAATCTTTTGGGTATATGGAAAGAATCTGAGTACTCATCTGGTCAATCTGTTCCCGTGGGTGGTCTTGCCTATTATATAACTGCACCATCAAGGTCATTCTAATTTTCTTATTCCTCGTCAAATCAGTTCTTTCTTTCTCTTTTTCTCAGCAATCAAGTTAATGATTTCATTCTAACATTTGATAATTGTTGCATCTTTTTGCAGCTTGGCTGATATGGCAGCCAATCCTTTCCATGCCCTGTTCTATCTTATTTTTATGTTGTCTGCATGTGCTCTTTTCTCAAAAACTTGGATTGAAGTATCAGGATCTTCTGCTCGAGATGTTGCTAAGCAGCTTAAGGTACTCGGTTTCTAAAATGTCAGATGTTATTCGGAACTTTAGATGTCCACTTTTTTTCAAAATGCTTGTCATTTATAGTTCCAAGATCTTTATCATCAAGATCTCTATATTCTAGTTGAACTGAAAATTATTTTTCTTCTCACCAAGATAATTAAGCATCAGAGAAACATTTGAACCTTAGGAATTTAAGTCAAGTGCATTTGAATCATGTTAGTTCAAGATATCATAGAAAGTTTATTAAGACGCAAAATACTCTTGCCGATGGCGCTTTCACTTCCTCTGAAGGTTTTCTGAGCTAAAGGTGGGAGAGAGAGAGAAGTCAGAAGTGTTCAAATGCCCTATTGTTGATTGGTGGTTTACATCATGAGTTGAAAATAGAGCTTGTTGTGTGATCTTGCGTGAAAAGATAAATCCTTTAACAGCTTAGTTCAAGTAACAACTTATGGAGATTAGCTCCATTTTCTGAAAAATGTTGTTCATTGTATATGATGATTTCAGGAACAACAAATGGTGATGCCTGGTCACCGCGAATCGAACCTTCAAAAAGAGTTGAACCGCTACATACCGACTGCAGCAGCATTTGGAGGCATCTGCATTGGTGCACTCACCGTGTTGGCTGATTTTATGGGCGCAATTGGTTCAGGAACAGGAATTCTTCTTGCAGTCACAATCATTTACCAGTATTTCGAAACTTTTGAAAAGGAAAAAGCCAGCGAGCTTGGCCTTTTTGGCTTC

mRNA sequence

ATGGGGGGTGGATTTCGAGTTCTTCACTTGGTCAGACCGTTTCTTTCGTTCCTCCCAGAAGTTCAGAGCGCTGACAGGAAAGTTCCATTCAGAGAGAAGGTTATTTATACAGTGATTTCGCTTTTCATTTTCTTGGTTTGTAGCCAACTACCTCTGTATGGCATACACTCCACAACGGGAGCAGATCCGTTTTATTGGATGCGTGTGATTCTTGCTTCAAATCGTGGCACTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTGATGCAGCTCTTAGTTGGGTCTAAGATCATTGAAGTTGACAACAATGTGAGAGAGGACCGCGCACTCTTAAATGGGGCACAAAAATTGCTCGGTATACTCATAGCAATTGGGGAGGCGGTTGCTTATGTTCTCTCTGGCATGTACGGCAGTGTTGATCAACTTGGAGTGGGCAATGCCATTCTTATTATTGTTCAGCTCTGTTTTGCTGGTATCATCGTCATTTGTTTAGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACCAATATCTGTGAGAACATTATTTGGAAAGCATTTAGTCCCACTACAATCAATAGTGGACGAGGAGCTGAATTTGAAGGAGCTGTTATTGCTCTATTTCATCTGCTGATAGCTCGGTCAGACAAAGTTAGAGCACTTCGTGAGGCATTTTACCGACAAAATCTTCCCAATGTCACAAATCTGCTTGCAACAGTTTTTATCTTTTTAATTGTGATCTACTTCCAAGGATTCCGGGTGGTTTTGCCTGTGAGGTCAAAGAATGCCCGCGGACAACAAGGTTCATACCCAATCAAGCTGTTCTACACTTCCAACATGCCAATCATTCTACAATCTGCACTTGTATCAAATGTTTATTTCATCTCTCAGTTGCTGTACAGGAAGTATGGTGGAAACTTTCTCGTGAATCTTTTGGGTATATGGAAAGAATCTGAGTACTCATCTGGTCAATCTGTTCCCGTGGGTGGTCTTGCCTATTATATAACTGCACCATCAAGCTTGGCTGATATGGCAGCCAATCCTTTCCATGCCCTGTTCTATCTTATTTTTATGTTGTCTGCATGTGCTCTTTTCTCAAAAACTTGGATTGAAGTATCAGGATCTTCTGCTCGAGATGTTGCTAAGCAGCTTAAGGAACAACAAATGGTGATGCCTGGTCACCGCGAATCGAACCTTCAAAAAGAGTTGAACCGCTACATACCGACTGCAGCAGCATTTGGAGGCATCTGCATTGGTGCACTCACCGTGTTGGCTGATTTTATGGGCGCAATTGGTTCAGGAACAGGAATTCTTCTTGCAGTCACAATCATTTACCAGTATTTCGAAACTTTTGAAAAGGAAAAAGCCAGCGAGCTTGGCCTTTTTGGCTTC

Coding sequence (CDS)

ATGGGGGGTGGATTTCGAGTTCTTCACTTGGTCAGACCGTTTCTTTCGTTCCTCCCAGAAGTTCAGAGCGCTGACAGGAAAGTTCCATTCAGAGAGAAGGTTATTTATACAGTGATTTCGCTTTTCATTTTCTTGGTTTGTAGCCAACTACCTCTGTATGGCATACACTCCACAACGGGAGCAGATCCGTTTTATTGGATGCGTGTGATTCTTGCTTCAAATCGTGGCACTGTCATGGAACTTGGAATAACCCCCATTGTCACATCTGGGATGGTGATGCAGCTCTTAGTTGGGTCTAAGATCATTGAAGTTGACAACAATGTGAGAGAGGACCGCGCACTCTTAAATGGGGCACAAAAATTGCTCGGTATACTCATAGCAATTGGGGAGGCGGTTGCTTATGTTCTCTCTGGCATGTACGGCAGTGTTGATCAACTTGGAGTGGGCAATGCCATTCTTATTATTGTTCAGCTCTGTTTTGCTGGTATCATCGTCATTTGTTTAGATGAGCTTCTTCAGAAAGGATATGGCCTAGGCTCTGGAATTTCCCTCTTTATAGCTACCAATATCTGTGAGAACATTATTTGGAAAGCATTTAGTCCCACTACAATCAATAGTGGACGAGGAGCTGAATTTGAAGGAGCTGTTATTGCTCTATTTCATCTGCTGATAGCTCGGTCAGACAAAGTTAGAGCACTTCGTGAGGCATTTTACCGACAAAATCTTCCCAATGTCACAAATCTGCTTGCAACAGTTTTTATCTTTTTAATTGTGATCTACTTCCAAGGATTCCGGGTGGTTTTGCCTGTGAGGTCAAAGAATGCCCGCGGACAACAAGGTTCATACCCAATCAAGCTGTTCTACACTTCCAACATGCCAATCATTCTACAATCTGCACTTGTATCAAATGTTTATTTCATCTCTCAGTTGCTGTACAGGAAGTATGGTGGAAACTTTCTCGTGAATCTTTTGGGTATATGGAAAGAATCTGAGTACTCATCTGGTCAATCTGTTCCCGTGGGTGGTCTTGCCTATTATATAACTGCACCATCAAGCTTGGCTGATATGGCAGCCAATCCTTTCCATGCCCTGTTCTATCTTATTTTTATGTTGTCTGCATGTGCTCTTTTCTCAAAAACTTGGATTGAAGTATCAGGATCTTCTGCTCGAGATGTTGCTAAGCAGCTTAAGGAACAACAAATGGTGATGCCTGGTCACCGCGAATCGAACCTTCAAAAAGAGTTGAACCGCTACATACCGACTGCAGCAGCATTTGGAGGCATCTGCATTGGTGCACTCACCGTGTTGGCTGATTTTATGGGCGCAATTGGTTCAGGAACAGGAATTCTTCTTGCAGTCACAATCATTTACCAGTATTTCGAAACTTTTGAAAAGGAAAAAGCCAGCGAGCTTGGCCTTTTTGGCTTC

Protein sequence

MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANPFHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF
Homology
BLAST of MS021234 vs. NCBI nr
Match: XP_022140644.1 (protein transport protein Sec61 subunit alpha-like [Momordica charantia])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 474/476 (99.58%), Postives = 474/476 (99.58%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREPFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIY QGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYVQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 476

BLAST of MS021234 vs. NCBI nr
Match: XP_038897181.1 (protein transport protein Sec61 subunit alpha-like [Benincasa hispida])

HSP 1 Score: 902.5 bits (2331), Expect = 1.5e-258
Identity = 463/476 (97.27%), Postives = 470/476 (98.74%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQ+ADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLL GSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALREAFYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGVEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKY GNFLVNLLGIWKESEYS+GQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFLVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           +HA+FYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 YHAVFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELG FGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 476

BLAST of MS021234 vs. NCBI nr
Match: XP_008463755.1 (PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >XP_008463756.1 PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >KAA0066757.1 protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa] >TYK27904.1 protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 898.7 bits (2321), Expect = 2.2e-257
Identity = 459/476 (96.43%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRV+HL RPFLSFLPEVQ+ADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDR LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLG GNAILII+QLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALREAFYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKY GNF+VNLLGIWKESEYS+GQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHA+FYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKA+ELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of MS021234 vs. NCBI nr
Match: XP_030550124.1 (protein transport protein Sec61 subunit alpha-like [Rhodamnia argentea])

HSP 1 Score: 897.5 bits (2318), Expect = 4.9e-257
Identity = 457/476 (96.01%), Postives = 469/476 (98.53%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSG+VMQLL GSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLGVGNAILII+QLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGVGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICE IIWKAFSPTTINSGRGAEFEGAVIALFHLLI R+DKVRALREAFYRQ
Sbjct: 181 GISLFIATNICETIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLAT+ +FLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATILVFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRKY GNFLVNLLGIWKESEYSSGQSVPVGG+AYYITAPSSLADMAANP
Sbjct: 301 VSNIYFISQLLYRKYSGNFLVNLLGIWKESEYSSGQSVPVGGIAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYL+FMLSACALFSKTWIEVSGSSA+DVA+QL+EQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 476

BLAST of MS021234 vs. NCBI nr
Match: KAA0059055.1 (protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa] >TYK16267.1 protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa])

HSP 1 Score: 897.1 bits (2317), Expect = 6.5e-257
Identity = 458/476 (96.22%), Postives = 469/476 (98.53%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRK+PFREKVIYTVI+LFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVIALFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLGVGNAILIIVQLCFAGI+VICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVSQLGVGNAILIIVQLCFAGIVVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATN+CENIIWKAFSPTTINSGRGAEFEGAVIALFHLLI R+DKVRALREAFYRQ
Sbjct: 181 GISLFIATNMCENIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIV+YFQGFRVVLPVRSKN+RGQQGSYPIKLFYTSNMPIIL SAL
Sbjct: 241 NLPNVTNLLATVLIFLIVVYFQGFRVVLPVRSKNSRGQQGSYPIKLFYTSNMPIILHSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRKY GNFLVNLLGIWKESEYS+GQSVPVGGLAYYIT PSSLADMAANP
Sbjct: 301 VSNLYFISQLLYRKYSGNFLVNLLGIWKESEYSNGQSVPVGGLAYYITPPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEK SELGLFGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKVSELGLFGF 476

BLAST of MS021234 vs. ExPASy Swiss-Prot
Match: Q54XK2 (Protein transport protein Sec61 subunit alpha OS=Dictyostelium discoideum OX=44689 GN=sec61a PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.3e-196
Identity = 340/472 (72.03%), Postives = 403/472 (85.38%), Query Frame = 0

Query: 4   GFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           GFR L +V+PF S +PEV   DRK+PFREKV++T I LFIFLVCSQ+PLYGI ST  +DP
Sbjct: 2   GFRFLDIVKPFTSLVPEVGQPDRKIPFREKVLWTAICLFIFLVCSQIPLYGIRSTDSSDP 61

Query: 64  FYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLG 123
           FYW +VI+ASNRGT+MELGI+PIVTSGMVMQLL G+K+IE+D +V+ DR L + AQKL G
Sbjct: 62  FYWAKVIMASNRGTLMELGISPIVTSGMVMQLLAGAKLIEIDQSVKADRDLFSAAQKLFG 121

Query: 124 ILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGIS 183
           +LI +G+ VAY+ SG YG    LG GN  LI++QL FAGIIV+ LDELLQKGYG+GSGIS
Sbjct: 122 MLICVGQGVAYIWSGSYGDPAVLGFGNCFLIVLQLFFAGIIVMLLDELLQKGYGIGSGIS 181

Query: 184 LFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLP 243
           LFIATNICE I+WK FSPTT++ G+G EFEGAVIALFHLL+ R+DKVRAL+EAFYRQNLP
Sbjct: 182 LFIATNICETIVWKTFSPTTVSVGKGTEFEGAVIALFHLLLTRNDKVRALKEAFYRQNLP 241

Query: 244 NVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSN 303
           N+TNLLATV IF++VIYFQGFRV LPV+S    GQQG+YPIKLFYTSN+PIILQSALVSN
Sbjct: 242 NITNLLATVLIFMVVIYFQGFRVDLPVKSTRVSGQQGTYPIKLFYTSNIPIILQSALVSN 301

Query: 304 VYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANPFHA 363
           +YFISQLLYR++  N LVNL G W+ SEYS  Q +PV GL YYI++P++++ + A+PFHA
Sbjct: 302 LYFISQLLYRRFPDNILVNLFGAWRTSEYSQ-QMIPVSGLTYYISSPNNMSAVLADPFHA 361

Query: 364 LFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAA 423
           LFY+ FML++CALFSK WIEVSGSSARDVAKQLK+QQM M GHR++++ KELNRYIPTAA
Sbjct: 362 LFYITFMLTSCALFSKVWIEVSGSSARDVAKQLKDQQMTMKGHRDTSVIKELNRYIPTAA 421

Query: 424 AFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFG 476
           AFGG+CIGALTV+ADFMGAIGSGTGILLAVTIIYQYFETF KE+    G  G
Sbjct: 422 AFGGLCIGALTVVADFMGAIGSGTGILLAVTIIYQYFETFVKEQQELSGGIG 472

BLAST of MS021234 vs. ExPASy Swiss-Prot
Match: Q2KHX4 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Bos taurus OX=9913 GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 654.4 bits (1687), Expect = 9.6e-187
Identity = 328/471 (69.64%), Postives = 395/471 (83.86%), Query Frame = 0

Query: 4   GFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L +++PF + LPE+Q  +RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLG 123
           FYWMRVILASNRGT+MELGI+PIVTSG++MQLL G+KIIEV  +  +DRAL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 ILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGIS 183
           ++I IG+A+ YV++GMYG   ++G G  +LII+QL  AG+IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLP 243
           LFIATNICE I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DKVRALREAFYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSN 303
           N+ NL+ATVF+F +VIYFQGFRV LP++S   RGQ  SYPIKLFYTSN+PIILQSALVSN
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 VYFISQLLYRKYGGNFLVNLLGIWKE-SEYSSGQSVPVGGLAYYITAPSSLADMAANPFH 363
           +Y ISQ+L  ++ GNFLVNLLG W + S     +S PVGGL YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 ALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTA 423
            + Y+IFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGL 474
           AAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQYFE F KE+A   G+
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQAEVGGM 471

BLAST of MS021234 vs. ExPASy Swiss-Prot
Match: Q9H9S3 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Homo sapiens OX=9606 GN=SEC61A2 PE=2 SV=3)

HSP 1 Score: 654.4 bits (1687), Expect = 9.6e-187
Identity = 328/471 (69.64%), Postives = 395/471 (83.86%), Query Frame = 0

Query: 4   GFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L +++PF + LPE+Q  +RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLG 123
           FYWMRVILASNRGT+MELGI+PIVTSG++MQLL G+KIIEV  +  +DRAL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 ILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGIS 183
           ++I IG+A+ YV++GMYG   ++G G  +LII+QL  AG+IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLP 243
           LFIATNICE I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DKVRALREAFYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSN 303
           N+ NL+ATVF+F +VIYFQGFRV LP++S   RGQ  SYPIKLFYTSN+PIILQSALVSN
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 VYFISQLLYRKYGGNFLVNLLGIWKE-SEYSSGQSVPVGGLAYYITAPSSLADMAANPFH 363
           +Y ISQ+L  ++ GNFLVNLLG W + S     +S PVGGL YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 ALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTA 423
            + Y+IFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGL 474
           AAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQYFE F KE+A   G+
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQAEVGGM 471

BLAST of MS021234 vs. ExPASy Swiss-Prot
Match: Q9JLR1 (Protein transport protein Sec61 subunit alpha isoform 2 OS=Mus musculus OX=10090 GN=Sec61a2 PE=2 SV=3)

HSP 1 Score: 654.4 bits (1687), Expect = 9.6e-187
Identity = 328/471 (69.64%), Postives = 395/471 (83.86%), Query Frame = 0

Query: 4   GFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADP 63
           G + L +++PF + LPE+Q  +RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADP
Sbjct: 2   GIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 61

Query: 64  FYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLG 123
           FYWMRVILASNRGT+MELGI+PIVTSG++MQLL G+KIIEV  +  +DRAL NGAQKL G
Sbjct: 62  FYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFG 121

Query: 124 ILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGIS 183
           ++I IG+A+ YV++GMYG   ++G G  +LII+QL  AG+IV+ LDELLQKGYGLGSGIS
Sbjct: 122 MIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 181

Query: 184 LFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLP 243
           LFIATNICE I+WKAFSPTTIN+GRG EFEGAVIALFHLL  R+DKVRALREAFYRQNLP
Sbjct: 182 LFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLP 241

Query: 244 NVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSN 303
           N+ NL+ATVF+F +VIYFQGFRV LP++S   RGQ  SYPIKLFYTSN+PIILQSALVSN
Sbjct: 242 NLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSN 301

Query: 304 VYFISQLLYRKYGGNFLVNLLGIWKE-SEYSSGQSVPVGGLAYYITAPSSLADMAANPFH 363
           +Y ISQ+L  ++ GNFLVNLLG W + S     +S PVGGL YY++ P S+  +  +P H
Sbjct: 302 LYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVH 361

Query: 364 ALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTA 423
            + Y+IFML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHR++++  ELNRYIPTA
Sbjct: 362 VVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA 421

Query: 424 AAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGL 474
           AAFGG+CIGAL+VLADF+GAIGSGTGILLAVTIIYQYFE F KE+A   G+
Sbjct: 422 AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQAEVGGM 471

BLAST of MS021234 vs. ExPASy Swiss-Prot
Match: Q90ZM2 (Protein transport protein Sec61 subunit alpha-like 1 OS=Danio rerio OX=7955 GN=sec61al1 PE=2 SV=3)

HSP 1 Score: 653.3 bits (1684), Expect = 2.1e-186
Identity = 327/473 (69.13%), Postives = 398/473 (84.14%), Query Frame = 0

Query: 6   RVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTGADPFY 65
           + L +++PF + LPE+Q  +RK+ FREKV++T I+LFIFLVC Q+PL+GI S+  ADPFY
Sbjct: 4   KFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFY 63

Query: 66  WMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQKLLGIL 125
           WMRVILASNRGT+MELGI+PIVTSG++MQLL G+KIIEV  +  +DRAL NGAQKL G++
Sbjct: 64  WMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV-GDTPKDRALFNGAQKLFGMI 123

Query: 126 IAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGSGISLF 185
           I IG+A+ YV++GMYG   ++G G  +LII+QL  AG+IV+ LDELLQKGYGLGSGISLF
Sbjct: 124 ITIGQAIVYVMTGMYGDPSEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISLF 183

Query: 186 IATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQNLPNV 245
           IATNICE I+WKAFSPTT+N+GRG EFEGA+IALFHLL  R+DKVRALREAFYRQNLPN+
Sbjct: 184 IATNICETIVWKAFSPTTVNTGRGTEFEGAIIALFHLLATRTDKVRALREAFYRQNLPNL 243

Query: 246 TNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSNVY 305
            NL+ATVF+F +VIYFQGFRV LP++S   RGQ  +YPIKLFYTSN+PIILQSALVSN+Y
Sbjct: 244 MNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSNLY 303

Query: 306 FISQLLYRKYGGNFLVNLLGIWKESEYSSG---QSVPVGGLAYYITAPSSLADMAANPFH 365
            ISQ+L  ++ GNFLVNLLG W  S+ SSG   ++ PVGGL YY++ P S   +  +P H
Sbjct: 304 VISQMLSTRFSGNFLVNLLGTW--SDTSSGGPARAYPVGGLCYYLSPPESFGSVLDDPVH 363

Query: 366 ALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTA 425
           A+ Y++FML +CA FSKTWIEVSGSSA+DVAKQLKEQQMVM GHRE+++  ELNRYIPTA
Sbjct: 364 AVIYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA 423

Query: 426 AAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFG 476
           AAFGG+CIG L+V+ADF+GAIGSGTGILLAVTIIYQYFE F KE+ SE+G  G
Sbjct: 424 AAFGGLCIGGLSVMADFLGAIGSGTGILLAVTIIYQYFEIFVKEQ-SEVGSMG 472

BLAST of MS021234 vs. ExPASy TrEMBL
Match: A0A6J1CG91 (protein transport protein Sec61 subunit alpha-like OS=Momordica charantia OX=3673 GN=LOC111011251 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.5e-263
Identity = 474/476 (99.58%), Postives = 474/476 (99.58%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALRE FYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREPFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIY QGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYVQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 476

BLAST of MS021234 vs. ExPASy TrEMBL
Match: A0A1S3CK18 (protein transport protein Sec61 subunit alpha-like OS=Cucumis melo OX=3656 GN=LOC103501823 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 459/476 (96.43%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRV+HL RPFLSFLPEVQ+ADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDR LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLG GNAILII+QLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALREAFYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKY GNF+VNLLGIWKESEYS+GQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHA+FYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKA+ELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of MS021234 vs. ExPASy TrEMBL
Match: A0A5A7VI03 (Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00890 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 459/476 (96.43%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRV+HL RPFLSFLPEVQ+ADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVIHLARPFLSFLPEVQNADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDR LLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRTLLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLG GNAILII+QLCFAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVGQLGAGNAILIIIQLCFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICENIIWKAFSPTTINSGRG EFEGAVIALFHLLI RSDK+RALREAFYRQ
Sbjct: 181 GISLFIATNICENIIWKAFSPTTINSGRGIEFEGAVIALFHLLIIRSDKIRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSNVYFISQLLYRKY GNF+VNLLGIWKESEYS+GQSVPVGGLAYYITAPSSLADMAANP
Sbjct: 301 VSNVYFISQLLYRKYSGNFIVNLLGIWKESEYSAGQSVPVGGLAYYITAPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHA+FYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHAMFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKA+ELG FGF
Sbjct: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKANELGFFGF 476

BLAST of MS021234 vs. ExPASy TrEMBL
Match: A0A5D3CWJ5 (Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G00360 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.1e-257
Identity = 458/476 (96.22%), Postives = 469/476 (98.53%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRK+PFREKVIYTVI+LFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVIALFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYGSV QLGVGNAILIIVQLCFAGI+VICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGSVSQLGVGNAILIIVQLCFAGIVVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATN+CENIIWKAFSPTTINSGRGAEFEGAVIALFHLLI R+DKVRALREAFYRQ
Sbjct: 181 GISLFIATNMCENIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIV+YFQGFRVVLPVRSKN+RGQQGSYPIKLFYTSNMPIIL SAL
Sbjct: 241 NLPNVTNLLATVLIFLIVVYFQGFRVVLPVRSKNSRGQQGSYPIKLFYTSNMPIILHSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRKY GNFLVNLLGIWKESEYS+GQSVPVGGLAYYIT PSSLADMAANP
Sbjct: 301 VSNLYFISQLLYRKYSGNFLVNLLGIWKESEYSNGQSVPVGGLAYYITPPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEK SELGLFGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKVSELGLFGF 476

BLAST of MS021234 vs. ExPASy TrEMBL
Match: A0A5A7UT26 (Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G00330 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.1e-257
Identity = 457/476 (96.01%), Postives = 469/476 (98.53%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFLSFLPEVQSADRK+PFREKVIYTVI+LFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKIPFREKVIYTVIALFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIA+GEAVAYVLSGMYGSV QLGVGNAILIIVQLCFAGI+VICLDELLQKGYGLGS
Sbjct: 121 LLGILIAVGEAVAYVLSGMYGSVSQLGVGNAILIIVQLCFAGIVVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATN+CENIIWKAFSPTTINSGRGAEFEGAVIALFHLLI R+DKVRALREAFYRQ
Sbjct: 181 GISLFIATNMCENIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIV+YFQGFRVVLPVRSKN+RGQQGSYPIKLFYTSNMPIIL SAL
Sbjct: 241 NLPNVTNLLATVLIFLIVVYFQGFRVVLPVRSKNSRGQQGSYPIKLFYTSNMPIILHSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRKY GNFLVNLLGIWKESEYS+GQSVPVGGLAYYIT PSSLADMAANP
Sbjct: 301 VSNLYFISQLLYRKYSGNFLVNLLGIWKESEYSNGQSVPVGGLAYYITPPSSLADMAANP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYL+FMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEK SELGLFGF
Sbjct: 421 TAAAFGGMCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKVSELGLFGF 476

BLAST of MS021234 vs. TAIR 10
Match: AT2G34250.1 (SecY protein transport family protein )

HSP 1 Score: 861.3 bits (2224), Expect = 3.7e-250
Identity = 442/476 (92.86%), Postives = 461/476 (96.85%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFL+FLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSG+VMQLL GSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYG V QLGVGNAILII+QL FAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICE+IIWKAFSPTTIN+GRGAEFEGAVIALFH+LI +S+KV ALR+AFYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRK+ GNF VNLLG WKESEY SGQS+PV GLAY ITAP+S +DMAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFSDMAAHP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFY++FML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELG FGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

BLAST of MS021234 vs. TAIR 10
Match: AT2G34250.2 (SecY protein transport family protein )

HSP 1 Score: 861.3 bits (2224), Expect = 3.7e-250
Identity = 442/476 (92.86%), Postives = 461/476 (96.85%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFL+FLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSG+VMQLL GSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYG V QLGVGNAILII+QL FAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICE+IIWKAFSPTTIN+GRGAEFEGAVIALFH+LI +S+KV ALR+AFYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRK+ GNF VNLLG WKESEY SGQS+PV GLAY ITAP+S +DMAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFSDMAAHP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFY++FML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELG FGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

BLAST of MS021234 vs. TAIR 10
Match: AT1G29310.1 (SecY protein transport family protein )

HSP 1 Score: 860.1 bits (2221), Expect = 8.2e-250
Identity = 441/476 (92.65%), Postives = 461/476 (96.85%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           MGGGFRVLHLVRPFL+FLPEVQSADRK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MGGGFRVLHLVRPFLAFLPEVQSADRKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILASNRGTVMELGITPIVTSG+VMQLL GSKIIEVDNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIAIGEAVAYVLSGMYG V QLGVGNAILII+QL FAGIIVICLDELLQKGYGLGS
Sbjct: 121 LLGILIAIGEAVAYVLSGMYGPVGQLGVGNAILIILQLFFAGIIVICLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICE+IIWKAFSPTTIN+GRGAEFEGAVIALFH+LI +S+KV ALR+AFYRQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINTGRGAEFEGAVIALFHMLITKSNKVAALRQAFYRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNLLATV IFLIVIYFQGFRVVLPVRSK+ARGQQGSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLLATVLIFLIVIYFQGFRVVLPVRSKSARGQQGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQLLYRK+ GNF VNLLG WKESEY SGQS+PV GLAY ITAP+S ADMAA+P
Sbjct: 301 VSNLYFISQLLYRKFSGNFFVNLLGQWKESEY-SGQSIPVSGLAYLITAPASFADMAAHP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFY++FML+ACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP
Sbjct: 361 FHALFYIVFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFGF 477
           TAAAFGG+CIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELG FGF
Sbjct: 421 TAAAFGGVCIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFGF 475

BLAST of MS021234 vs. TAIR 10
Match: AT1G78720.1 (SecY protein transport family protein )

HSP 1 Score: 854.7 bits (2207), Expect = 3.4e-248
Identity = 432/475 (90.95%), Postives = 462/475 (97.26%), Query Frame = 0

Query: 1   MGGGFRVLHLVRPFLSFLPEVQSADRKVPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60
           M GGFRV+HLVRPFL+FLPEVQS +RK+PFREKVIYTVISLFIFLVCSQLPLYGIHSTTG
Sbjct: 1   MVGGFRVIHLVRPFLAFLPEVQSPERKIPFREKVIYTVISLFIFLVCSQLPLYGIHSTTG 60

Query: 61  ADPFYWMRVILASNRGTVMELGITPIVTSGMVMQLLVGSKIIEVDNNVREDRALLNGAQK 120
           ADPFYWMRVILAS+RGTVMELGITPIVTSGMVMQLL GSKIIE+DNNVREDRALLNGAQK
Sbjct: 61  ADPFYWMRVILASSRGTVMELGITPIVTSGMVMQLLAGSKIIEIDNNVREDRALLNGAQK 120

Query: 121 LLGILIAIGEAVAYVLSGMYGSVDQLGVGNAILIIVQLCFAGIIVICLDELLQKGYGLGS 180
           LLGILIA+G+AVAYVLSGMYGSV +LGVGNAILIIVQLCFA IIV+CLDELLQKGYGLGS
Sbjct: 121 LLGILIAVGQAVAYVLSGMYGSVGELGVGNAILIIVQLCFAAIIVLCLDELLQKGYGLGS 180

Query: 181 GISLFIATNICENIIWKAFSPTTINSGRGAEFEGAVIALFHLLIARSDKVRALREAFYRQ 240
           GISLFIATNICE+IIWKAFSPTTINSGRGA+FEGAVIALFHLLI R+DKVRALREAF+RQ
Sbjct: 181 GISLFIATNICESIIWKAFSPTTINSGRGAQFEGAVIALFHLLITRTDKVRALREAFFRQ 240

Query: 241 NLPNVTNLLATVFIFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSAL 300
           NLPNVTNL ATV IFLIVIYFQGFRVVLPVRSKNARGQ+GSYPIKLFYTSNMPIILQSAL
Sbjct: 241 NLPNVTNLHATVLIFLIVIYFQGFRVVLPVRSKNARGQRGSYPIKLFYTSNMPIILQSAL 300

Query: 301 VSNVYFISQLLYRKYGGNFLVNLLGIWKESEYSSGQSVPVGGLAYYITAPSSLADMAANP 360
           VSN+YFISQ+LYRK+GGNFLVNL+G WKESEY SGQS+PVGG+AYYITAPSSLA+MA +P
Sbjct: 301 VSNIYFISQILYRKFGGNFLVNLIGTWKESEY-SGQSIPVGGIAYYITAPSSLAEMATHP 360

Query: 361 FHALFYLIFMLSACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIP 420
           FHALFYL+FML+ACALFSKTWIEVSGSSA+DVA+QL+EQQMVMPGHR+SNLQKELNRYIP
Sbjct: 361 FHALFYLVFMLAACALFSKTWIEVSGSSAKDVARQLREQQMVMPGHRDSNLQKELNRYIP 420

Query: 421 TAAAFGGICIGALTVLADFMGAIGSGTGILLAVTIIYQYFETFEKEKASELGLFG 476
           TAAAFGG+CIGALTVLAD MGAIGSGTGILLAVTIIYQYFETFEKEKASELG FG
Sbjct: 421 TAAAFGGLCIGALTVLADLMGAIGSGTGILLAVTIIYQYFETFEKEKASELGFFG 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140644.11.3e-26299.58protein transport protein Sec61 subunit alpha-like [Momordica charantia][more]
XP_038897181.11.5e-25897.27protein transport protein Sec61 subunit alpha-like [Benincasa hispida][more]
XP_008463755.12.2e-25796.43PREDICTED: protein transport protein Sec61 subunit alpha-like [Cucumis melo] >XP... [more]
XP_030550124.14.9e-25796.01protein transport protein Sec61 subunit alpha-like [Rhodamnia argentea][more]
KAA0059055.16.5e-25796.22protein transport protein Sec61 subunit alpha-like [Cucumis melo var. makuwa] >T... [more]
Match NameE-valueIdentityDescription
Q54XK22.3e-19672.03Protein transport protein Sec61 subunit alpha OS=Dictyostelium discoideum OX=446... [more]
Q2KHX49.6e-18769.64Protein transport protein Sec61 subunit alpha isoform 2 OS=Bos taurus OX=9913 GN... [more]
Q9H9S39.6e-18769.64Protein transport protein Sec61 subunit alpha isoform 2 OS=Homo sapiens OX=9606 ... [more]
Q9JLR19.6e-18769.64Protein transport protein Sec61 subunit alpha isoform 2 OS=Mus musculus OX=10090... [more]
Q90ZM22.1e-18669.13Protein transport protein Sec61 subunit alpha-like 1 OS=Danio rerio OX=7955 GN=s... [more]
Match NameE-valueIdentityDescription
A0A6J1CG916.5e-26399.58protein transport protein Sec61 subunit alpha-like OS=Momordica charantia OX=367... [more]
A0A1S3CK181.1e-25796.43protein transport protein Sec61 subunit alpha-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7VI031.1e-25796.43Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa O... [more]
A0A5D3CWJ53.1e-25796.22Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa O... [more]
A0A5A7UT264.1e-25796.01Protein transport protein Sec61 subunit alpha-like OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT2G34250.13.7e-25092.86SecY protein transport family protein [more]
AT2G34250.23.7e-25092.86SecY protein transport family protein [more]
AT1G29310.18.2e-25092.65SecY protein transport family protein [more]
AT1G78720.13.4e-24890.95SecY protein transport family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023201SecY domain superfamilyGENE3D1.10.3370.10SecY subunit domaincoord: 9..474
e-value: 1.0E-170
score: 570.1
IPR023201SecY domain superfamilySUPERFAMILY103491Preprotein translocase SecY subunitcoord: 10..466
IPR002208SecY/SEC61-alpha familyPFAMPF00344SecYcoord: 77..459
e-value: 6.0E-75
score: 252.4
IPR002208SecY/SEC61-alpha familyPIRSFPIRSF004557SecY_Sec61alphacoord: 8..472
e-value: 3.5E-98
score: 327.0
IPR002208SecY/SEC61-alpha familyTIGRFAMTIGR00967TIGR00967coord: 31..468
e-value: 1.4E-108
score: 361.4
IPR002208SecY/SEC61-alpha familyPANTHERPTHR10906SECY/SEC61-ALPHA FAMILY MEMBERcoord: 1..476
IPR019561Translocon Sec61/SecY, plug domainPFAMPF10559Plug_transloconcoord: 42..76
e-value: 6.2E-18
score: 64.4
NoneNo IPR availablePANTHERPTHR10906:SF31F9K20.24 PROTEINcoord: 1..476
IPR030659SecY conserved sitePROSITEPS00755SECY_1coord: 77..96

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS021234.1MS021234.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0016020 membrane