MS021219 (gene) Bitter gourd (TR) v1

BLAST of MS021219 vs. NCBI nr
Match: XP_022131763.1 (protein SRG1-like [Momordica charantia])

HSP 1 Score: 696.4 bits (1796), Expect = 1.2e-196
Identity = 345/348 (99.14%), Postives = 346/348 (99.43%), Query Frame = 0

Query: 1   SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD 60
           SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD
Sbjct: 47  SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD 106

Query: 61  SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQAF 120
           SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEE+KKLWQIEGDVEGFGQAF
Sbjct: 107 SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEERKKLWQIEGDVEGFGQAF 166

Query: 121 VVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER 180
           VVSEEQKLDWSDIFFLCTAPEH RKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER
Sbjct: 167 VVSEEQKLDWSDIFFLCTAPEHQRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER 226

Query: 181 ALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLKI 240
           ALEIKEGEMTEMFKDGNQSMRMNYYPACPE EKVIGLTPHSDAVGLTILLQINQVEGLKI
Sbjct: 227 ALEIKEGEMTEMFKDGNQSMRMNYYPACPETEKVIGLTPHSDAVGLTILLQINQVEGLKI 286

Query: 241 KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK 300
           KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK
Sbjct: 287 KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK 346

Query: 301 LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 349
           LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP
Sbjct: 347 LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 394

BLAST of MS021219 vs. NCBI nr
Match: XP_038897143.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 553.5 bits (1425), Expect = 1.3e-153
Identity = 272/351 (77.49%), Postives = 307/351 (87.46%), Query Frame = 0

Query: 1   SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRS-PIVSAASEIPVIDFASLLSGASM 60
           +S YGSSI VPSVQELAKQP  EIPPRY+R DLL   PI SAASEIPVID ++  S A++
Sbjct: 9   TSRYGSSILVPSVQELAKQPITEIPPRYIRPDLLHHVPIASAASEIPVIDMSNFHSDATI 68

Query: 61  DSESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGF 120
           DSE ARLHSAC++WGFFQLVNHGV    IEKM+IET++ F LPIEEKKKLWQ EGDVEGF
Sbjct: 69  DSELARLHSACKNWGFFQLVNHGVSDSLIEKMKIETKKLFDLPIEEKKKLWQREGDVEGF 128

Query: 121 GQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVR 180
           GQAFVVSEEQKLDWSD+FF+ T+P H RK RLFQNLPL+FR  LEDYS+EVKD+AAAI  
Sbjct: 129 GQAFVVSEEQKLDWSDLFFIATSPSHLRKQRLFQNLPLTFRKTLEDYSAEVKDLAAAIFE 188

Query: 181 ELERALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 240
            +E+ L IK+GE+ EMFKDGNQSMRMNYYP CPEPEK IGLTPHSDAVGLTILLQIN+VE
Sbjct: 189 GVEKVLGIKKGELKEMFKDGNQSMRMNYYPRCPEPEKAIGLTPHSDAVGLTILLQINEVE 248

Query: 241 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 300
           GLKIKK+G WIPV PLP+AF+VN+GDIMEM+TNG+Y+SIEHCATVNS+SERLSIATF NP
Sbjct: 249 GLKIKKDGNWIPVMPLPNAFVVNVGDIMEMVTNGEYKSIEHCATVNSKSERLSIATFNNP 308

Query: 301 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINH 348
           S EK IRP PSLITPHSPPLF +LTYQ +V GLFT+KLDGKSYLDAMRI++
Sbjct: 309 SMEKEIRPIPSLITPHSPPLFKTLTYQEFVKGLFTQKLDGKSYLDAMRIHY 359

BLAST of MS021219 vs. NCBI nr
Match: XP_008463796.1 (PREDICTED: probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Cucumis melo])

HSP 1 Score: 536.6 bits (1381), Expect = 1.6e-148
Identity = 265/348 (76.15%), Postives = 300/348 (86.21%), Query Frame = 0

Query: 4   YGSSIPVPSVQELAKQPFIEIPPRYVRSDLL-RSPIVSAASEIPVIDFASLLSGASMDSE 63
           YGSSI VPSVQELAK P  EIP RY+  DLL  SPI S AS+IPVID ++L S  +MDSE
Sbjct: 11  YGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMSNLRSHDTMDSE 70

Query: 64  SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQA 123
            +RLHSAC++WGFFQLV HGV    +E+M++ETQ+ F+LPIEEKKKLWQ EGDVEGFGQA
Sbjct: 71  LSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQREGDVEGFGQA 130

Query: 124 FVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 183
           FV SEEQKLDW DIFF+ T P H RKPRLFQNLPLS R  LE+YS+ VKDVAA I+  +E
Sbjct: 131 FVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVKDVAAEILDGVE 190

Query: 184 RALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLK 243
           +AL IKEGE++E+FKDGNQSMRMNYYP CPEPEKVIGLTPHSDA GLTILLQIN+VEGLK
Sbjct: 191 KALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTILLQINKVEGLK 250

Query: 244 IKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFE 303
           IKKEG WI V PLP+AFIVNIGDI+EM+TNG+Y+SIEHCATVNS+SERLSIATF +PS E
Sbjct: 251 IKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERLSIATFNSPSLE 310

Query: 304 KLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINH 348
           K IRP PSLITPHSPPLFT+LTYQ YV G+F+RKLDGKSYLDAMRI+H
Sbjct: 311 KEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIHH 358

BLAST of MS021219 vs. NCBI nr
Match: XP_004146011.3 (LOW QUALITY PROTEIN: protein SRG1 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 8.0e-148
Identity = 261/349 (74.79%), Postives = 298/349 (85.39%), Query Frame = 0

Query: 4   YGSSIPVPSVQELAKQPFIEIPPRYVRSDLL-RSPIVSAASEIPVIDFASLLSGASMDSE 63
           +GSSI VPSVQELAK P  EIP RY+R DLL  SPI SAAS+IPVID ++  S  +MDSE
Sbjct: 12  FGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFRSNDTMDSE 71

Query: 64  SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQA 123
            +RLHSAC++WGFFQLV HGV    +E+M++ETQ+ F+LPIEEKKKLWQ EGDVEGFGQA
Sbjct: 72  LSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQA 131

Query: 124 FVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 183
           F+ SEEQKLDW DIFF+ T+P H R PRLFQNLPLS R  LE+YS+ VKDV  AI+  +E
Sbjct: 132 FITSEEQKLDWCDIFFIATSPSHFRNPRLFQNLPLSLRETLEEYSAAVKDVTTAIIGGIE 191

Query: 184 RALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLK 243
           +AL IKEGEM+E+FKDGNQSMR+NYYP CPEPEKVIGLTPHSD+VGLTILLQIN+VEGLK
Sbjct: 192 KALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEGLK 251

Query: 244 IKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFE 303
           IKKEG WI V PLP+AFIVNIGDI+EM+TNGKY+SIEHCATVNS+SERLSIATF  PS E
Sbjct: 252 IKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPSLE 311

Query: 304 KLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 349
           K IRP PSLITPHSPPLF +LTYQ YV GLF+R LDGK+YLDAMRI+ P
Sbjct: 312 KEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360

BLAST of MS021219 vs. NCBI nr
Match: KGN55530.2 (hypothetical protein Csa_012011 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 8.0e-148
Identity = 261/349 (74.79%), Postives = 298/349 (85.39%), Query Frame = 0

Query: 4   YGSSIPVPSVQELAKQPFIEIPPRYVRSDLL-RSPIVSAASEIPVIDFASLLSGASMDSE 63
           +GSSI VPSVQELAK P  EIP RY+R DLL  SPI SAAS+IPVID ++  S  +MDSE
Sbjct: 12  FGSSILVPSVQELAKHPITEIPHRYIRPDLLHHSPIASAASQIPVIDMSNFRSNDTMDSE 71

Query: 64  SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQA 123
            +RLHSAC++WGFFQLV HGV    +E+M++ETQ+ F+LPIEEKKKLWQ EGDVEGFGQA
Sbjct: 72  LSRLHSACKNWGFFQLVKHGVSDSLMERMKMETQKLFQLPIEEKKKLWQREGDVEGFGQA 131

Query: 124 FVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 183
           F+ SEEQKLDW DIFF+ T+P H R PRLFQNLPLS R  LE+YS+ VKDV  AI+  +E
Sbjct: 132 FITSEEQKLDWCDIFFIATSPSHFRNPRLFQNLPLSLRETLEEYSAAVKDVTTAIIGGIE 191

Query: 184 RALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLK 243
           +AL IKEGEM+E+FKDGNQSMR+NYYP CPEPEKVIGLTPHSD+VGLTILLQIN+VEGLK
Sbjct: 192 KALGIKEGEMSELFKDGNQSMRINYYPRCPEPEKVIGLTPHSDSVGLTILLQINEVEGLK 251

Query: 244 IKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFE 303
           IKKEG WI V PLP+AFIVNIGDI+EM+TNGKY+SIEHCATVNS+SERLSIATF  PS E
Sbjct: 252 IKKEGNWITVMPLPNAFIVNIGDILEMVTNGKYKSIEHCATVNSKSERLSIATFYTPSLE 311

Query: 304 KLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 349
           K IRP PSLITPHSPPLF +LTYQ YV GLF+R LDGK+YLDAMRI+ P
Sbjct: 312 KEIRPTPSLITPHSPPLFRTLTYQEYVKGLFSRTLDGKTYLDAMRIHPP 360

BLAST of MS021219 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 8.2e-111
Identity = 203/349 (58.17%), Postives = 256/349 (73.35%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQPFI-EIPPRYVRSDLLRSPI---VSAASEIPVIDFASLLSGASMDS 65
           SSI VPSVQE+ K+  I  +PPRYVRSD  ++ +        EIP+ID   L S  +MDS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 66  ESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQ 125
           E  +L  AC++WGFFQLVNHG+    ++K++ E Q+FF LP+EEKKK WQ   ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 126 AFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVREL 185
           AFVVSE+QKLDW+D+FF    P   RKP LF  LPL FR+ LE YSSEV+ VA  ++ ++
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 186 ERALEIKEGEMTEMFKDGN--QSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 245
            RALEIK  E+ ++F D +  QSMRMNYYP CP+P++VIGLTPHSD+VGLT+L+Q+N VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 246 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 305
           GL+IKK+GKW+PV PLP+AFIVNIGD++E+ITNG YRSIEH   VNSE ERLSIATF N 
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 306 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
              K + PA SL+       F  LT + Y  GLF+R LDGK+YLDA+RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MS021219 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.9e-100
Identity = 188/355 (52.96%), Postives = 252/355 (70.99%), Query Frame = 0

Query: 5   GSSIPVPSVQELAKQPFIEIPPRYVRS-DLLRSPIVSAASE----IPVIDFASLLSGASM 64
           G+ + +PSVQELAK    EIP RY+ + + L+ P+ ++  +    +PVID  +L+S   +
Sbjct: 10  GNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSEPV 69

Query: 65  DS--ESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVE 124
               E  RLHSAC++WGFFQ+VNHGV    ++ ++ + Q FF L + EK K  Q +GDVE
Sbjct: 70  TEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDVE 129

Query: 125 GFGQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAI 184
           GFGQAFV SE+Q LDW+DIF + T P H RKP LF  LPL  R  +E YSSE+K ++  +
Sbjct: 130 GFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMVL 189

Query: 185 VRELERALEIKE---GEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQ 244
             ++E+AL+++     E++E+FKD  Q MRMNYYP CP+PE  IGLTPHSD  GLTILLQ
Sbjct: 190 FEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQ 249

Query: 245 INQVEGLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIA 304
           +N+VEGL+IK EG+WI V PLP+AF+VN+GD++E++TNG YRS++H A VNS  ERLSIA
Sbjct: 250 LNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIA 309

Query: 305 TFCNPSFEKLIRPAPSLITPHSPPLFTS-LTYQHYVSGLFTRKLDGKSYLDAMRI 346
           TF +P+ E  I P  SLITP++P LF S  TY   V    +RKLDGKS+LD+MR+
Sbjct: 310 TFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of MS021219 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.2e-99
Identity = 189/355 (53.24%), Postives = 248/355 (69.86%), Query Frame = 0

Query: 5   GSSIPVPSVQELAKQPFIEIPPRYVRSD-----LLRSPIVSAASEIPVIDFASLLSGASM 64
           G+ + +PSVQELAK    EIP RYV ++      + + +++    IPVID  +LLS   +
Sbjct: 10  GNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSPEPI 69

Query: 65  --DSESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVE 124
               E  RLH AC++WGFFQ+VNHGV    ++ ++ E Q FF L ++EK K  Q +GDVE
Sbjct: 70  IGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDGDVE 129

Query: 125 GFGQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAI 184
           GFGQ F+ SE+Q LDW+DIF + T P H RKP LF  LP+  R  +E YSSE+K ++  +
Sbjct: 130 GFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMKKLSMVL 189

Query: 185 VRELERALEIKEGE---MTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQ 244
             ++E+AL+++  E   M+E+F DG Q+MRMNYYP CP+P   IGLT HSD  GLTILLQ
Sbjct: 190 FNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTILLQ 249

Query: 245 INQVEGLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIA 304
           IN+VEGL+IK+EG WI V PLP+AF+VN+GDI+E++TNG Y S++H A VNS +ERLSIA
Sbjct: 250 INEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERLSIA 309

Query: 305 TFCNPSFEKLIRPAPSLITPHSPPLFTS-LTYQHYVSGLFTRKLDGKSYLDAMRI 346
           TF +PS E +I P  SLITP +P LF S  TY   V    TRKLDGKS+LD+MRI
Sbjct: 310 TFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMRI 364

BLAST of MS021219 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.7e-95
Identity = 181/353 (51.27%), Postives = 243/353 (68.84%), Query Frame = 0

Query: 5   GSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPI------VSAASEIPVIDFASLLSGAS 64
           G+ + +PSVQELAK    EIP RY  +    SP+      V+    +PVID  +LLS   
Sbjct: 10  GNGLSIPSVQELAKLTLAEIPSRYTCTG--ESPLNNIGASVTDDETVPVIDLQNLLSPEP 69

Query: 65  M--DSESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDV 124
           +    E  +LHSAC++WGFFQLVNHGV    ++ ++ E + FF LP+ EK K  Q +GD 
Sbjct: 70  VVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDF 129

Query: 125 EGFGQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAA 184
           EGFGQ ++ SE+Q+LDW+++F + + P H RKP LF  LPL FR  LE Y S++K ++  
Sbjct: 130 EGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTV 189

Query: 185 IVRELERALEIKEGE-MTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQI 244
           +   LE++L++ E + MT++F+DG Q+MRMNYYP CP PE V+GLT HSD  GLTILLQ+
Sbjct: 190 VFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQL 249

Query: 245 NQVEGLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIAT 304
           N+VEGL+I+KE +WI + PLPDAFIVN+GDI+E++TNG YRS+EH A VNS  ERLSIAT
Sbjct: 250 NEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIAT 309

Query: 305 FCNPSFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
           F +   E  I P  SL+TP +P LF    Y+  +    +RKLDGKS+LD MR+
Sbjct: 310 FHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of MS021219 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-77
Identity = 151/348 (43.39%), Postives = 220/348 (63.22%), Query Frame = 0

Query: 5   GSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIV--SAASEIPVIDFASLLSGASMDSE 64
           G S+PV +VQ LA +    +P RYVR +L    +V    + EIPVID + LL       E
Sbjct: 9   GGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACDE 68

Query: 65  SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQA 124
            A+ HSAC DWGFFQL+NHGV+   IEKM+++T++FF+LP +EK    Q+   +EG+GQA
Sbjct: 69  LAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYGQA 128

Query: 125 FVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 184
           FV SEEQKLDW+D+ FL T P   R  R +   P SFR  +E YS E++ VA  +   + 
Sbjct: 129 FVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCLTGMMA 188

Query: 185 RALEIKEGEMTEMFKD--GNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEG 244
           + L ++   +T+  +     +   +    +C E    +GL+PHSDA GLT+L+Q+N+V G
Sbjct: 189 KNLGLESEILTKPLRTVFNREDELLPSMSSCGEG---LGLSPHSDATGLTLLIQVNEVNG 248

Query: 245 LKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPS 304
           L IKK+ KW+P+ P+  AF+VNIGD++E+++NG Y+SIEH A +N++ ERLSIA F +P 
Sbjct: 249 LHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSIAAFHDPE 308

Query: 305 FEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
           +   I P P L+  +    + ++ Y+ Y+      KLDGKS LD M++
Sbjct: 309 YGTKIGPLPDLVKENGVK-YKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

BLAST of MS021219 vs. ExPASy TrEMBL
Match: A0A6J1BUE5 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 5.9e-197
Identity = 345/348 (99.14%), Postives = 346/348 (99.43%), Query Frame = 0

Query: 1   SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD 60
           SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD
Sbjct: 47  SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD 106

Query: 61  SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQAF 120
           SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEE+KKLWQIEGDVEGFGQAF
Sbjct: 107 SESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEERKKLWQIEGDVEGFGQAF 166

Query: 121 VVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER 180
           VVSEEQKLDWSDIFFLCTAPEH RKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER
Sbjct: 167 VVSEEQKLDWSDIFFLCTAPEHQRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELER 226

Query: 181 ALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLKI 240
           ALEIKEGEMTEMFKDGNQSMRMNYYPACPE EKVIGLTPHSDAVGLTILLQINQVEGLKI
Sbjct: 227 ALEIKEGEMTEMFKDGNQSMRMNYYPACPETEKVIGLTPHSDAVGLTILLQINQVEGLKI 286

Query: 241 KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK 300
           KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK
Sbjct: 287 KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK 346

Query: 301 LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 349
           LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP
Sbjct: 347 LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 394

BLAST of MS021219 vs. ExPASy TrEMBL
Match: A0A1S3CK11 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=LOC103501850 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.9e-149
Identity = 265/348 (76.15%), Postives = 300/348 (86.21%), Query Frame = 0

Query: 4   YGSSIPVPSVQELAKQPFIEIPPRYVRSDLL-RSPIVSAASEIPVIDFASLLSGASMDSE 63
           YGSSI VPSVQELAK P  EIP RY+  DLL  SPI S AS+IPVID ++L S  +MDSE
Sbjct: 11  YGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMSNLRSHDTMDSE 70

Query: 64  SARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQA 123
            +RLHSAC++WGFFQLV HGV    +E+M++ETQ+ F+LPIEEKKKLWQ EGDVEGFGQA
Sbjct: 71  LSRLHSACKNWGFFQLVKHGVSDSLVERMKMETQKLFELPIEEKKKLWQREGDVEGFGQA 130

Query: 124 FVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELE 183
           FV SEEQKLDW DIFF+ T P H RKPRLFQNLPLS R  LE+YS+ VKDVAA I+  +E
Sbjct: 131 FVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSAAVKDVAAEILDGVE 190

Query: 184 RALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLK 243
           +AL IKEGE++E+FKDGNQSMRMNYYP CPEPEKVIGLTPHSDA GLTILLQIN+VEGLK
Sbjct: 191 KALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAGLTILLQINKVEGLK 250

Query: 244 IKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFE 303
           IKKEG WI V PLP+AFIVNIGDI+EM+TNG+Y+SIEHCATVNS+SERLSIATF +PS E
Sbjct: 251 IKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKSERLSIATFNSPSLE 310

Query: 304 KLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINH 348
           K IRP PSLITPHSPPLFT+LTYQ YV G+F+RKLDGKSYLDAMRI+H
Sbjct: 311 KEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRIHH 358

BLAST of MS021219 vs. ExPASy TrEMBL
Match: A0A6J1F704 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.6e-146
Identity = 261/352 (74.15%), Postives = 300/352 (85.23%), Query Frame = 0

Query: 1   SSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMD 60
           +S +G SI VPSVQELAKQP IE+P RY+R DL   PIVSAASEIPVID ++  S ASM 
Sbjct: 7   TSRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPIVSAASEIPVIDMSNFHSDASMH 66

Query: 61  SESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKK-LWQIEGDVEGF 120
           SE ARLHSAC++WGFFQLVNHGV    IEKM+ ETQ FF LPIEEKKK LWQ E D EGF
Sbjct: 67  SELARLHSACKNWGFFQLVNHGVSDSLIEKMKAETQMFFNLPIEEKKKRLWQSEEDAEGF 126

Query: 121 GQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVR 180
           GQAFVVSEEQKLDW D+FF+ TAP H RKPRLFQNLPL+FRN +E+YS+EVK++A AI  
Sbjct: 127 GQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSAEVKNLAMAIFE 186

Query: 181 ELERALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 240
           + E+AL IKEGE+TE+FKDG+QSMRMNYYP CPEPEKVIGLTPHSDAVGLTILLQIN++E
Sbjct: 187 QFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVGLTILLQINEME 246

Query: 241 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 300
           GLKIKK+G W+ V PLP+AFIVNIGDIME+ITNG+Y+SIEHCATVN++ ERLSIATF NP
Sbjct: 247 GLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAKRERLSIATFNNP 306

Query: 301 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 349
             EK I+  PSLITPHSPPLFT+LTYQ +V  LF+RKLDGKS+LD +RI+ P
Sbjct: 307 RLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRIHTP 358

BLAST of MS021219 vs. ExPASy TrEMBL
Match: A0A1U8AYK0 (protein SRG1-like OS=Nelumbo nucifera OX=4432 GN=LOC104608961 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.5e-125
Identity = 222/351 (63.25%), Postives = 273/351 (77.78%), Query Frame = 0

Query: 3   TYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVS---AASEIPVIDFASLLSGASM 62
           T GSSI VP VQELAKQ  + +PPRYVRS     PI+S   +   IPVID  SLLS  SM
Sbjct: 13  TLGSSILVPCVQELAKQHLVIVPPRYVRSQ--HPPIISNPDSIPTIPVIDMQSLLSEESM 72

Query: 63  DSESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGF 122
           DSE +RLH+AC+DWGFFQLVNHGV    +EK ++E QEFF LP+EEKKK WQ++ ++EGF
Sbjct: 73  DSELSRLHAACKDWGFFQLVNHGVSSSLVEKTKLEIQEFFNLPLEEKKKFWQLQNEIEGF 132

Query: 123 GQAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVR 182
           GQAFVVSE+QKLDW D+FF+ T P H RKP LF  LPL  R+ L+ YS E+K +A  I+ 
Sbjct: 133 GQAFVVSEDQKLDWGDLFFMSTLPTHRRKPHLFPKLPLPLRDTLDSYSQEMKRLAMNILE 192

Query: 183 ELERALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 242
            + +AL+++ GE+ E+F+DG QSMRMNYYP CPEPEK IGLTPHSDA GLTILLQ+N++E
Sbjct: 193 LMAKALKMEVGEIRELFEDGMQSMRMNYYPPCPEPEKTIGLTPHSDASGLTILLQVNEME 252

Query: 243 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 302
           GL+++K+G W+PV PLP+AFI+NIGDI+E+ TNG YRSIEH ATVNS  ERLSIATFCNP
Sbjct: 253 GLQVRKDGMWVPVKPLPNAFIINIGDILEIFTNGTYRSIEHQATVNSVKERLSIATFCNP 312

Query: 303 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINH 348
           +F+K I PAPSLI+  +P LF  +  + Y  GLF RKLDGKSY+D MRI +
Sbjct: 313 NFDKEIGPAPSLISSGNPALFRRVASEEYFKGLFARKLDGKSYIDVMRIQN 361

BLAST of MS021219 vs. ExPASy TrEMBL
Match: A0A061FGF8 (Senescence-related gene 1 OS=Theobroma cacao OX=3641 GN=TCM_032151 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.7e-119
Identity = 211/344 (61.34%), Postives = 262/344 (76.16%), Query Frame = 0

Query: 5   GSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPIVSAASEIPVIDFASLLSGASMDSESA 64
           G+S+ VP VQELAK+    +PPRY+R DL ++ + +   EIPVID  SLL   SMDSE A
Sbjct: 8   GNSLLVPCVQELAKETMTTLPPRYLRPDLEQTTVPADGLEIPVIDMKSLLCDESMDSELA 67

Query: 65  RLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQAFV 124
           +L  AC++WGFFQLVNHGV    +EK + E Q+FF LP+EEK+K WQ +G+VEGFGQ+FV
Sbjct: 68  KLDYACKEWGFFQLVNHGVSSSLMEKTKTEIQDFFNLPMEEKQKFWQYQGEVEGFGQSFV 127

Query: 125 VSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELERA 184
           VSEEQKLDW D+FF+ + P H RKP LF NLPL  R+ L+ YS E+K ++  ++  + +A
Sbjct: 128 VSEEQKLDWGDLFFMTSLPPHFRKPHLFPNLPLPLRDTLDSYSLELKTLSMTLLTNMAKA 187

Query: 185 LEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLKIK 244
           L+++  EM EMF+ G QS RMNYYP CP+PE+ IGL PHSDAVGLTILLQ+NQVEGL+IK
Sbjct: 188 LDMRVEEMNEMFEGGMQSFRMNYYPPCPQPEQAIGLRPHSDAVGLTILLQLNQVEGLQIK 247

Query: 245 KEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEKL 304
           K+GKW PV PLPDAFIVN+GDI+EM+TNG YRSIEH ATVN E ERLSIATF +P ++  
Sbjct: 248 KDGKWTPVKPLPDAFIVNVGDILEMVTNGAYRSIEHRATVNCERERLSIATFWSPRYDGE 307

Query: 305 IRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
           + PAPSLI+P  P LF  L  + Y  GLF R+L GKSYLD MRI
Sbjct: 308 VGPAPSLISPEKPVLFKKLRVEEYFKGLFARELQGKSYLDTMRI 351

BLAST of MS021219 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 408.7 bits (1049), Expect = 4.8e-114
Identity = 203/345 (58.84%), Postives = 256/345 (74.20%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQPFIE--IPPRYVRSDLLR--SPIVSAASEIPVIDFASLLSGASMDS 65
           SS+ VPSVQE+ K+  I   +PPRYVRSD  +  + I S  ++IP+ID + L S  SMDS
Sbjct: 9   SSVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDS 68

Query: 66  ESARLHSACRDWGFFQLVNHGVKIEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQAFV 125
           E  +L  AC++WGFFQLVNHG+ ++K + + Q+FF LP+EEKKKLWQ  GD+EGFGQAFV
Sbjct: 69  EIDKLDFACKEWGFFQLVNHGMDLDKFKSDIQDFFNLPMEEKKKLWQQPGDIEGFGQAFV 128

Query: 126 VSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRELERA 185
            SEEQKLDW+D+FFL   P   RKP LF  LPL FR+ L+ YS+E+K +A  +  +L  A
Sbjct: 129 FSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVLFAKLASA 188

Query: 186 LEIKEGEMTEMFKDG-NQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGLKI 245
           L+IK  EM ++F D   Q +RMNYYP CPEP+K IGLTPHSDA GLTILLQ+N+VEGL+I
Sbjct: 189 LKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEGLQI 248

Query: 246 KKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSFEK 305
           KK+GKW+ V PLP+A +VN+GDI+E+ITNG YRSIEH   VNSE ERLS+A+F N  F K
Sbjct: 249 KKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTGFGK 308

Query: 306 LIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
            I P  SL+  H   LF +LT + Y  GLF+R+LDGK+YLD MRI
Sbjct: 309 EIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of MS021219 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 401.7 bits (1031), Expect = 5.8e-112
Identity = 203/349 (58.17%), Postives = 256/349 (73.35%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQPFI-EIPPRYVRSDLLRSPI---VSAASEIPVIDFASLLSGASMDS 65
           SSI VPSVQE+ K+  I  +PPRYVRSD  ++ +        EIP+ID   L S  +MDS
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 66  ESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQ 125
           E  +L  AC++WGFFQLVNHG+    ++K++ E Q+FF LP+EEKKK WQ   ++EGFGQ
Sbjct: 70  EVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQ 129

Query: 126 AFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVREL 185
           AFVVSE+QKLDW+D+FF    P   RKP LF  LPL FR+ LE YSSEV+ VA  ++ ++
Sbjct: 130 AFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKM 189

Query: 186 ERALEIKEGEMTEMFKDGN--QSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 245
            RALEIK  E+ ++F D +  QSMRMNYYP CP+P++VIGLTPHSD+VGLT+L+Q+N VE
Sbjct: 190 ARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVE 249

Query: 246 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 305
           GL+IKK+GKW+PV PLP+AFIVNIGD++E+ITNG YRSIEH   VNSE ERLSIATF N 
Sbjct: 250 GLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNV 309

Query: 306 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
              K + PA SL+       F  LT + Y  GLF+R LDGK+YLDA+RI
Sbjct: 310 GMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MS021219 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 3.9e-108
Identity = 197/348 (56.61%), Postives = 251/348 (72.13%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQPFI-EIPPRYVRSDLLRSPIV---SAASEIPVIDFASLLSGASMDS 65
           SSI VPSVQE+ K+  I  +PPRYVRSD   + I       ++IP+ID + L S  SMDS
Sbjct: 9   SSIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDS 68

Query: 66  ESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQ 125
           E  +L SAC++WGFFQLVNHG++   + K++ E Q+FF LP+EEKK LWQ   ++EGFGQ
Sbjct: 69  EIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGFGQ 128

Query: 126 AFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVREL 185
            FVVSEEQKLDW+D+FFL   P   RKP LF  LPL FR+ L+ YS+EVK +A  ++ ++
Sbjct: 129 VFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLGKI 188

Query: 186 ERALEIKEGEMTEMFKDG-NQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEG 245
             AL+IK  EM ++F D   Q +R+NYYP CPEP+KVIGLTPHSD+ GLTILLQ N+VEG
Sbjct: 189 AVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEVEG 248

Query: 246 LKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPS 305
           L+IKK  KW+ V PLP+A +VN+GDI+E+ITNG YRSIEH   VNSE ERLS+A F N  
Sbjct: 249 LQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHNIG 308

Query: 306 FEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
             K I P  SL+  H    F S+T + Y +GLF+R+LDGK+YLD MR+
Sbjct: 309 LGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of MS021219 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.3e-107
Identity = 196/347 (56.48%), Postives = 248/347 (71.47%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQP-FIEIPPRYVRSDLLRSPIV---SAASEIPVIDFASLLSGASMDS 65
           SS+ VP V E+ K+  F  IPPRYVR D  ++ I+   S +SEIPVID   L S ++MDS
Sbjct: 10  SSLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDS 69

Query: 66  ESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFGQ 125
           E  +L  AC+DWGFFQLVNHG+    +EK+  E QEFF LP++EK+KLWQ  G+ EGFGQ
Sbjct: 70  ELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEGFGQ 129

Query: 126 AFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVREL 185
             +VSE QKLDW D+F L T P   RK  LF  LP  FR  LE YSSEVK +A  +  ++
Sbjct: 130 VNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKM 189

Query: 186 ERALEIKEGEMTEMFKDGNQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVEGL 245
              LEIK  EM ++F D  QS+++NYYP CP+P++V+GLT HSDA GLTILLQ+NQVEGL
Sbjct: 190 ASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGL 249

Query: 246 KIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNPSF 305
           +IKK+GKW+ V PL DA +VN+G+I+E+ITNG+YRSIEH A VNSE ERLS+A F +P  
Sbjct: 250 QIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHSPGK 309

Query: 306 EKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
           E +IRPA SL+      LF S++ Q Y    FT+KL+GKS+LD MRI
Sbjct: 310 ETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

BLAST of MS021219 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 9.0e-105
Identity = 197/349 (56.45%), Postives = 250/349 (71.63%), Query Frame = 0

Query: 6   SSIPVPSVQELAKQPFI-EIPPRYVRSDLLRSPIV----SAASEIPVIDFASLLSGASMD 65
           SSI VPSVQE+ K   I  +PPRYVR D  ++ +V       SEIP+ID   L S  ++D
Sbjct: 10  SSILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVD 69

Query: 66  SESARLHSACRDWGFFQLVNHGVK---IEKMRIETQEFFKLPIEEKKKLWQIEGDVEGFG 125
           SE  +L  AC+++GFFQLVNHG+    ++K++ E Q+FF LP+EEKKKLWQ    +EGFG
Sbjct: 70  SEVEKLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGFG 129

Query: 126 QAFVVSEEQKLDWSDIFFLCTAPEHHRKPRLFQNLPLSFRNALEDYSSEVKDVAAAIVRE 185
           QAFVVSE+QKLDW+D+FFL   P   RK  LF  LPL FR+ L+ YS+ VK +A  ++ +
Sbjct: 130 QAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLAK 189

Query: 186 LERALEIKEGEMTEMFKDG-NQSMRMNYYPACPEPEKVIGLTPHSDAVGLTILLQINQVE 245
           + +AL+IK  E+ E+F D   QSMRMNYYP CP+P  V GL PHSDAVGLTILLQ+N+V+
Sbjct: 190 MAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEVD 249

Query: 246 GLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERLSIATFCNP 305
           GL+IKK GKW  V PL +AFIVN+GD++E+ITNG YRSIEH A VN E ERLSIATF N 
Sbjct: 250 GLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHNT 309

Query: 306 SFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRI 346
             +K I PA SL+       F SL  + Y++GLF+R+L GK+YLDAMRI
Sbjct: 310 GMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRI 358