MS021082 (gene) Bitter gourd (TR) v1

Overview


Name	MS021082
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	vacuolar protein sorting-associated protein 25
Location	scaffold290: 1092191 .. 1094920 (+)
RNA-Seq Expression	MS021082
Synteny	MS021082

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAATTGGGAGATTTCAAGCTGCCACACTTCTTCAATTACCCCCCATATTTCACGTAATTTTCTTTTTCTGCCTCAGTTACCATTGTTCTTACATCAGTATGAGCTACATCAAGTTAGTTAAAAGTTCAGTGAATCAATATCTGATCATGTTGATATCTTGTTTTTGACGGCTATTTTCAGAGCGAAATAAACTGACACTTCATTCTCAACCAGTAAAGTTACATAACAGTAGTTACATAACACTAAAGGATAAACTTAGATACTTACTGGCAAGTAGGTCTCTTACTTACCATTATTAAAAATTGACAAGAGAAATGAATGCATAAAGGAATAGAGAGGAAGTAGAATAAATACTGGTCTCTTACTCACCATTATTAAAAATTGACAAGAGAAATGAATGCATAAAGGAATAGAGAGGAAATAGAATAAATACTTGTAAATTTTTTAATAGTGATAAGTAAGAAGATACCTACTTACCAGTAAGCACCTAAGTTTTATCCCTAACAGAATCCTGTATCACTTATAGTCTCATAAATAAGAATGTGATAATTTTTACCTTGCTGCAATTCCTGAAATTGTTACTAGTAACCTTGATTGTTTCACTTTTTTTCCTTTTTTTTTCAATCTTCTCTTTTTGTCTTGAGTTGCAAATGTAATCTTCTATTGCATGTCTTCTCAGTTTGCAACCAGTGAGGGACACCCGCGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTTGACTATTGCCGAACACAAAAGATATTTGTCATCGGACTTGAAGAGGAATTTCCTATATTTACCAATACTGCCATCGAGAGTAAATTCCATGAACTTATTTGGCCCTAATTGGTTGCAAATCTTTTACCATTTGCATTAATATCAACCTTAAAATGGCCATTGACATTAGCATCATGAACAGTCTGAAAAAGATGTAAGATATTGGCCTCATTATATGCAGCCTTTCACTCTATGGAGGATAGAATATCATTAGAATTGGTGTATAGGGCTCTCAGTCATGAAGCTATGTGTTACTATTTCAGTTTATTTGATGTATTTTACAGTGTGATTGTGCTGCTACAGGATCTCTCAGTCATGAAGCTAGAGAAGCATTTCTCTCAGCCTTAGTTTCAGATGGTCTGGCCCTGCTTATTTAGTTCTATAATCCTATTTGTTTAGAGTCTCCATTTCATTTCCTAAGGGAAAAACTGATATCTGTTGCTTTCATTTGTCCAATATAGGTCGCGCAGAATGGCTGGATAAGGGACATAGAAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGATTAAATACCAAGTTAAATTTTTGGTTAAAAAACACTTTTGGTTTCATTCTAGGAACGATTTAATCCCTTAAAACTAATGATTTAGTCCCTATACTTTTAAATTGATTATAATTTAGTCCCTGAACTTTAGTATGTAACAATTTAGTCGCCGTAGTTTAAAATTTGTAATAATTTAGTCCTTATTGTGAAAAATCTCATCAAAATTATGTGTCAATTTCTATTATATAATTGTTTAGGCTTTGTAGTTTATAAACACATTTGGTCGCTTGTCATTTTATCTGTCATTAAGCTTAACAACATTTTTTTACAATAGAGACTAAATCGTTACAAATTTTAAACTACACAGACTAAATCGTTACATACTAAAGTTCAGGAATTAAATTATTTCAAATTTGGAAGTACAATGACTGAATTGTTATAAGTGGATGGTTTTGAAATTTTTACTGTCTAACTAAATAGGTGAAAAACAATGGACTTGAAGATAGTGTAATGACGGTTGAGGAAATGCGTTCAGGAGTTGAATCTAGAGGAACAGGTATTCCCATGCATTTTCTGAGGTGATTTAGATATTCGAATGATTTTCAATTGGTAATTTTAGGTTTAAATCTGATTTTGATATTTAAATTTGTTTTTGTACTCGTAAAAATTTCTATTTTGATTTCTGAATTTCGTTTTGGTCCTTATTGTCAAATTTTACACCAAACCGATTCTTAAAACATAACTTATGTGAGAAAATTTTTTTTTAAATGCTCTAAGAAATATACATAGTACAGAACTAAAACAAATTTAGTTAAATAACAAGTTTAGTTTCTCAACTTTGGTCTAGTAGATGCATGAAACTCGAAAAGTATTTAATAAGTCCGCAAATTTTCAATTTTGTCTCAAATAAGTCAATTGAAATTGGAAAAACGTGTGTAATAGGTCTCTAAATTTTCTATTTGGTGTCTAATACGTCCTTGATCTCATTGATGTTTTTAAAATTTCATGGATGTAATAGATGATATACAATTTAATTTTGTATCTAATAGAATTTTGACCTTTTAATTTTGGTAGGTTATAATCCGTCCTTGAAATTTTTAAAATTTTTAAAAATTGGAACATTTGGGAGCTCAAAAATTGAAACAAATAAACGTAAACATCCATTAGAGTTAGATTATATGGTTGCGATGCTGTTTGTTTCTGCTTAAATATTACATCCTGAAATTACATTGAAACCTCATTTTTCACAGACCAAAATTACTCTTATTTTTTCTTCTGTTTTATGCATCAGAGCTTCAAGGGATTGATCGTACGATTCTAATGAGAGCGTTGAAACTGCTTGAGCAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGAATTAAATTCTCTGTA

mRNA sequence

ATGCAGAAATTGGGAGATTTCAAGCTGCCACACTTCTTCAATTACCCCCCATATTTCACTTTGCAACCAGTGAGGGACACCCGCGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTTGACTATTGCCGAACACAAAAGATATTTGTCATCGGACTTGAAGAGGAATTTCCTATATTTACCAATACTGCCATCGAGAGATCTCTCAGTCATGAAGCTAGAGAAGCATTTCTCTCAGCCTTAGTTTCAGATGGTCGCGCAGAATGGCTGGATAAGGGACATAGAAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAACAATGGACTTGAAGATAGTGTAATGACGGTTGAGGAAATGCGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACGATTCTAATGAGAGCGTTGAAACTGCTTGAGCAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGAATTAAATTCTCTGTA

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTGCCACACTTCTTCAATTACCCCCCATATTTCACTTTGCAACCAGTGAGGGACACCCGCGAGAAGCAAATTCAACTTTGGAAGGAACTTATTCTTGACTATTGCCGAACACAAAAGATATTTGTCATCGGACTTGAAGAGGAATTTCCTATATTTACCAATACTGCCATCGAGAGATCTCTCAGTCATGAAGCTAGAGAAGCATTTCTCTCAGCCTTAGTTTCAGATGGTCGCGCAGAATGGCTGGATAAGGGACATAGAAAATGCCTTATTTTGTGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAACAATGGACTTGAAGATAGTGTAATGACGGTTGAGGAAATGCGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACGATTCTAATGAGAGCGTTGAAACTGCTTGAGCAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGAATTAAATTCTCTGTA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIFTNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV

Homology

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BLAST of MS021082 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 366.3 bits (939), Expect = 1.5e-97
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 357.1 bits (915), Expect = 9.1e-95
Identity = 172/179 (96.09%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. NCBI nr
Match: XP_038891553.1 (vacuolar protein sorting-associated protein 25 [Benincasa hispida])

HSP 1 Score: 357.1 bits (915), Expect = 9.1e-95
Identity = 171/179 (95.53%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSEIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMT+EE+RSGVESRGTELQGIDRT+LMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTIEEIRSGVESRGTELQGIDRTVLMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 355.1 bits (910), Expect = 3.4e-94
Identity = 170/179 (94.97%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 353.2 bits (905), Expect = 1.3e-93
Identity = 171/179 (95.53%), Postives = 176/179 (98.32%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 2.9e-88
Identity = 152/179 (84.92%), Postives = 170/179 (94.97%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FP+F
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+AI+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 7.1e-34
Identity = 69/173 (39.88%), Postives = 105/173 (60.69%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPIFTNTA 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  E P+F N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+ +  L  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]

BLAST of MS021082 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 2.7e-33
Identity = 69/173 (39.88%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPIFTNTA 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  E P+F N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA +     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of MS021082 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.6e-30
Identity = 66/188 (35.11%), Postives = 115/188 (61.17%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP----IFTN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       +F N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  TAIERSLSHEAREAFLSALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA ++ +  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV E+++G +S+  E   ++ TILM++LK+LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]

BLAST of MS021082 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.0e-29
Identity = 64/173 (36.99%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEEFPIFTNTA 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   +E P+F N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+ +  L  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]

BLAST of MS021082 vs. ExPASy TrEMBL
Match: A0A6J1CUP8 (vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN=LOC111014756 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 7.2e-98
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy TrEMBL
Match: A0A5A7UHJ5 (Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001240 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.4e-95
Identity = 172/179 (96.09%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy TrEMBL
Match: A0A1S3BKR8 (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.4e-95
Identity = 172/179 (96.09%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy TrEMBL
Match: A0A0A0L1R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.7e-94
Identity = 170/179 (94.97%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTEL+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. ExPASy TrEMBL
Match: A0A6J1L2J9 (vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111498518 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.3e-94
Identity = 171/179 (95.53%), Postives = 176/179 (98.32%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+ IERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of MS021082 vs. TAIR 10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 325.9 bits (834), Expect = 2.1e-89
Identity = 152/179 (84.92%), Postives = 170/179 (94.97%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FP+F
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+AI+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of MS021082 vs. TAIR 10
Match: AT4G19003.1 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 325.9 bits (834), Expect = 2.1e-89
Identity = 152/179 (84.92%), Postives = 170/179 (94.97%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FP+F
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +N+AI+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

The following BLAST results are available for this feature:

BLAST of MS021082 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145259.1	1.5e-97	100.00	vacuolar protein sorting-associated protein 25 [Momordica charantia]	[more]
XP_008448627.1	9.1e-95	96.09	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
XP_038891553.1	9.1e-95	95.53	vacuolar protein sorting-associated protein 25 [Benincasa hispida]	[more]
XP_004146105.1	3.4e-94	94.97	vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp...	[more]
XP_022949655.1	1.3e-93	95.53	vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557...	[more]

BLAST of MS021082 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZC9	2.9e-88	84.92	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6AX45	7.1e-34	39.88	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2...	[more]
Q6NWF4	2.7e-33	39.88	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P...	[more]
Q55GD9	3.6e-30	35.11	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44...	[more]
Q9CQ80	9.0e-29	36.99	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25...	[more]

BLAST of MS021082 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CUP8	7.2e-98	100.00	vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN...	[more]
A0A5A7UHJ5	4.4e-95	96.09	Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. m...	[more]
A0A1S3BKR8	4.4e-95	96.09	vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365...	[more]
A0A0A0L1R7	1.7e-94	94.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1	[more]
A0A6J1L2J9	6.3e-94	95.53	vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MS021082 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19003.2	2.1e-89	84.92	E2F/DP family winged-helix DNA-binding domain	[more]
AT4G19003.1	2.1e-89	84.92	E2F/DP family winged-helix DNA-binding domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008570	ESCRT-II complex, Vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 13..145 e-value: 2.4E-43 score: 147.8
IPR008570	ESCRT-II complex, Vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 1..179
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 103..178 e-value: 4.3E-25 score: 89.1
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	1.10.10.570		coord: 5..101 e-value: 1.6E-31 score: 110.6
None	No IPR available	PANTHER	PTHR13149:SF1	SUBFAMILY NOT NAMED	coord: 1..179
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 102..177
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 7..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS021082.1	MS021082.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071985	multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex