MS020735 (gene) Bitter gourd (TR) v1

Overview


Name	MS020735
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold178: 344182 .. 344451 (+)
RNA-Seq Expression	MS020735
Synteny	MS020735

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTTGATTGCAGAACTGATGGCGGTTCGGGAAGGGATTAGGCTTGCTGTACGGCCGAGTCTTGGATGGGTGGTGGTTCTCTCAGACTGTTTGGAAGCGATCAATCTTCTTCATGGGAGAGTTGTCTACCATTCAGAGACTGGTTTGTTGGTAGAGGAGATCTTGGAGCTGGAGAGTCATTTCTCAAGTATTGATTTCCTTCATGTAAATCGGAAGCATACTACGCTGGAAGATAGCTTAGCCAGACATGCGTCATTGTCCAAGACTTCT

mRNA sequence

CCTTTGATTGCAGAACTGATGGCGGTTCGGGAAGGGATTAGGCTTGCTGTACGGCCGAGTCTTGGATGGGTGGTGGTTCTCTCAGACTGTTTGGAAGCGATCAATCTTCTTCATGGGAGAGTTGTCTACCATTCAGAGACTGGTTTGTTGGTAGAGGAGATCTTGGAGCTGGAGAGTCATTTCTCAAGTATTGATTTCCTTCATGTAAATCGGAAGCATACTACGCTGGAAGATAGCTTAGCCAGACATGCGTCATTGTCCAAGACTTCT

Coding sequence (CDS)

CCTTTGATTGCAGAACTGATGGCGGTTCGGGAAGGGATTAGGCTTGCTGTACGGCCGAGTCTTGGATGGGTGGTGGTTCTCTCAGACTGTTTGGAAGCGATCAATCTTCTTCATGGGAGAGTTGTCTACCATTCAGAGACTGGTTTGTTGGTAGAGGAGATCTTGGAGCTGGAGAGTCATTTCTCAAGTATTGATTTCCTTCATGTAAATCGGAAGCATACTACGCTGGAAGATAGCTTAGCCAGACATGCGTCATTGTCCAAGACTTCT

Protein sequence

PLIAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFSSIDFLHVNRKHTTLEDSLARHASLSKTS

Homology

[Close]

BLAST of MS020735 vs. NCBI nr
Match: KAF4369473.1 (hypothetical protein G4B88_029571 [Cannabis sativa])

HSP 1 Score: 58.5 bits (140), Expect = 3.3e-05
Identity = 32/88 (36.36%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE  AV  G+RLA + ++   +V +DCL  I+ L      HS+ G+L  EIL L+ HF 
Sbjct: 649 LAEAAAVHLGVRLARKWAVSKAIVAADCLSIIDSLQSGSALHSDWGMLSREILSLKHHFL 708

Query: 63  SIDFLHVNRKHTTLEDSLARHASLSKTS 91
           S++F HV+R    + ++LA  + ++ +S
Sbjct: 709 SLNFTHVHRDCNAVANALAIWSRMTHSS 736

[Close]

BLAST of MS020735 vs. NCBI nr
Match: KAF4357254.1 (hypothetical protein G4B88_012621 [Cannabis sativa])

HSP 1 Score: 57.4 bits (137), Expect = 7.5e-05
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE +AV+ G+RLA + S+   +V +DCL  IN L      +S  G+L  +IL L+ HF 
Sbjct: 557 LAEAVAVKLGVRLAQKWSVSKAIVAADCLSIINSLQSSSALNSYWGMLCRDILSLKHHFL 616

Query: 63  SIDFLHVNR 72
           S++F HV+R
Sbjct: 617 SLNFTHVHR 625

[Close]

BLAST of MS020735 vs. NCBI nr
Match: KAF4355486.1 (hypothetical protein F8388_015240 [Cannabis sativa])

HSP 1 Score: 57.4 bits (137), Expect = 7.5e-05
Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE+ A+R GI LA   S+  ++V SDCL  IN L+      S+ GLL++EIL L+ HF 
Sbjct: 111 LAEVAAIRMGISLAHNKSIPKIIVASDCLGVINGLNSTSSTSSDWGLLIKEILPLQHHFI 170

Query: 63  SIDFLHVNRK 73
           S+ F ++ R+
Sbjct: 171 SVQFSYIPRQ 180

[Close]

BLAST of MS020735 vs. NCBI nr
Match: KAF7835740.1 (ribonuclease H [Senna tora])

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-04
Identity = 29/82 (35.37%), Postives = 47/82 (57.32%), Query Frame = 0


Query: 4    AELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFSS 63
            AELMA++ G+ L ++   G ++V  DCLEAINL+    +   + G L+++I  + + F+ 
Sbjct: 1383 AELMAIKHGLLLGLQRGFGDLIVEVDCLEAINLIKNEDISRHQLGSLIQDIRAISNSFAK 1442

Query: 64   IDFLHVNRKHTTLEDSLARHAS 86
            I   HV R+     D LA+  S
Sbjct: 1443 ISLKHVYREANHCADGLAKMGS 1464

[Close]

BLAST of MS020735 vs. NCBI nr
Match: KAF4384266.1 (hypothetical protein G4B88_016832 [Cannabis sativa])

HSP 1 Score: 53.9 bits (128), Expect = 8.2e-04
Identity = 31/89 (34.83%), Postives = 50/89 (56.18%), Query Frame = 0


Query: 2   LIAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHF 61
           L+AE+ A+R GI+LA R S    ++ SDC  AI  L     Y ++ G LV++I +  + F
Sbjct: 147 LLAEVTAIRLGIQLARRRSFSHAIIASDCQAAITTLQNNACYPTDWGQLVQDINQFRASF 206

Query: 62  SSIDFLHVNRKHTTLEDSLARHASLSKTS 91
             + F  VNR    + ++LA  + L++ S
Sbjct: 207 QYLRFSFVNRDCNKVANNLAHWSRLAQKS 235

[Close]

BLAST of MS020735 vs. ExPASy TrEMBL
Match: A0A803Q7N5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.3e-06
Identity = 32/88 (36.36%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 3    IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
            +AE +AV+ G+RLA + S+   +V +DCL  IN L      +S+ G+L  +IL L+ HF 
Sbjct: 1391 LAEAVAVKLGVRLAQKWSVSKAIVAADCLSIINSLQSSSALNSDWGMLCRDILSLKHHFL 1450

Query: 63   SIDFLHVNRKHTTLEDSLARHASLSKTS 91
            S++F HV+R    + ++LA  + ++ +S
Sbjct: 1451 SLNFTHVHRDCNAVANALAIWSRMTHSS 1478

[Close]

BLAST of MS020735 vs. ExPASy TrEMBL
Match: A0A7J6FFJ3 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_029571 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.6e-05
Identity = 32/88 (36.36%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE  AV  G+RLA + ++   +V +DCL  I+ L      HS+ G+L  EIL L+ HF 
Sbjct: 649 LAEAAAVHLGVRLARKWAVSKAIVAADCLSIIDSLQSGSALHSDWGMLSREILSLKHHFL 708

Query: 63  SIDFLHVNRKHTTLEDSLARHASLSKTS 91
           S++F HV+R    + ++LA  + ++ +S
Sbjct: 709 SLNFTHVHRDCNAVANALAIWSRMTHSS 736

[Close]

BLAST of MS020735 vs. ExPASy TrEMBL
Match: A0A7J6EAV9 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_015240 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.6e-05
Identity = 30/70 (42.86%), Postives = 45/70 (64.29%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE+ A+R GI LA   S+  ++V SDCL  IN L+      S+ GLL++EIL L+ HF 
Sbjct: 111 LAEVAAIRMGISLAHNKSIPKIIVASDCLGVINGLNSTSSTSSDWGLLIKEILPLQHHFI 170

Query: 63  SIDFLHVNRK 73
           S+ F ++ R+
Sbjct: 171 SVQFSYIPRQ 180

[Close]

BLAST of MS020735 vs. ExPASy TrEMBL
Match: A0A7J6EFZ9 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_012621 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.6e-05
Identity = 29/69 (42.03%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE +AV+ G+RLA + S+   +V +DCL  IN L      +S  G+L  +IL L+ HF 
Sbjct: 557 LAEAVAVKLGVRLAQKWSVSKAIVAADCLSIINSLQSSSALNSYWGMLCRDILSLKHHFL 616

Query: 63  SIDFLHVNR 72
           S++F HV+R
Sbjct: 617 SLNFTHVHR 625

[Close]

BLAST of MS020735 vs. ExPASy TrEMBL
Match: A0A7J6DLU8 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_011696 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-04
Identity = 28/67 (41.79%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 3   IAELMAVREGIRLAVRPSLGWVVVLSDCLEAINLLHGRVVYHSETGLLVEEILELESHFS 62
           +AE  A+R GI LA   S+  ++V SDC+  IN L+      S+ GLL++EIL L+ HF 
Sbjct: 492 LAEAAAIRMGISLAYNKSIPKIIVASDCMGVINGLNSTSSTASDWGLLIKEILSLQHHFI 551

Query: 63  SIDFLHV 70
           S+  L +
Sbjct: 552 SVQLLKI 558

The following BLAST results are available for this feature:

BLAST of MS020735 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF4369473.1	3.3e-05	36.36	hypothetical protein G4B88_029571 [Cannabis sativa]	[more]
KAF4357254.1	7.5e-05	42.03	hypothetical protein G4B88_012621 [Cannabis sativa]	[more]
KAF4355486.1	7.5e-05	42.86	hypothetical protein F8388_015240 [Cannabis sativa]	[more]
KAF7835740.1	1.7e-04	35.37	ribonuclease H [Senna tora]	[more]
KAF4384266.1	8.2e-04	34.83	hypothetical protein G4B88_016832 [Cannabis sativa]	[more]

BLAST of MS020735 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS020735 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A803Q7N5	4.3e-06	36.36	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A7J6FFJ3	1.6e-05	36.36	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_029571 PE=4 SV=1	[more]
A0A7J6EAV9	3.6e-05	42.86	RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_015240 PE=...	[more]
A0A7J6EFZ9	3.6e-05	42.03	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_012621 PE=4 SV=1	[more]
A0A7J6DLU8	4.0e-04	41.79	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_011696 PE=4 SV=1	[more]

BLAST of MS020735 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 2..89 e-value: 1.4E-8 score: 36.8
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..84 e-value: 5.2E-12 score: 45.7
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 1..82 e-value: 1.11095E-9 score: 49.2348
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 3..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020735.1	MS020735.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity