MS020723 (gene) Bitter gourd (TR) v1

Overview


Name	MS020723
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	scaffold178: 120032 .. 124416 (+)
RNA-Seq Expression	MS020723
Synteny	MS020723

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGCTATCAGAGGAGGAAATTACCAGGCTTTTCAGAATTCGAAAAACTGTGTTACAAATGCTAAAAGACCGAAGCTATTTTGTTGGAGACTTTGAGATTGACATGTCAAGAGAGCAATTTAAGCATAAATATGGTGAAAACATGAAAAGAGAAGATATTGTTATCAATAAAGCCAGGAGAAATGATAGCTCTGATCAGGTATTGTCTGATACAAAGGGATATTCATTAGTATTCTCATTGTTATTCAGCAACAGGAGCATACAAGTTTCTTGTTAATTCTCCTATTTGTTTTCTTCTTCTTATAGTGTCTTTTCTTCTGTCTAGATATATGTTTTCTTTCCAGAAGAACAGAAGGTTGGCGTAAAGACGATGAAAACTTATACCAATCGCATGAAATCGGAGAATGTCTTTAGAGCAATATTGGTCGTTCAACAAAATTTGACTCCCTTCGCTCGTACTTGTATAAGTGAAATATCTTCTAAATTCCACCTGGAAGTTTTCCAGGTGAAAATATTTCTTTAAAATATTTCATGGAGTATTTTAATTATTTACTTAGTTTATAAAAACAACACCTGTTGAAGTAAAAGTAGATTCACCATTCACCAGTAATTTGAGGAAAATCATTGGAACCTTCCTACTCCAAAAACTACTAATCATGTCATGCTCTCTCCCTGTAGTCCTTATTTTGCACATTACTAATGAGAATTAAAAATATGGGGGGTTGCTGGATACTTTGGTTGAAGAACAATATTATTAGTTATCGTGAATATATTGAAGCCCTCCCTGAGTTTGCTCTAAACTTTCCGCTGAAGTTAAATGACCGAGATAGTTGTTCAATTGCTCCCGCTTTTCTGAGCCTTAGATATAGAGGTTCTGGCTTGATTTCCAATCCTTGCTCCAACTTTTCTTAGCCTACAATAATCTTTTGTTCTTTTTCCTGTTTTCACTTGTCGATGCAGGAAGCTGAATTGCTAGTTAACATTAAAGATCACGTTCTTGTTCCCGAGCACCAGTTACTTACCAACGAAGAGAAGAAGACCCTGCTGGAGAGATATACCGTGAAAGAAACACAGGTCAGTATTAATTCTTTGGAAAATATGACATTGTGAGATTGCATCTTAGGAGCTATATTGTAATGTGATGTTCAATTCTGTTGTGTGTTTCAGATCCCATTAGATTACATACATTATTTACTGTGACTTAACCATATTTTGGTATTTTGTCTTCTGCTCCATTAAGTTTTCCTGGGAACAAAGTGGATCTATTGAGGTTGAAGTATATAAACCACAAGAAATTCGTTCATAATTATAGCCTCATTATGATTGTTAACAATTGGAAAGCTATTATTGTTTAGTCTTTGGGAGAGGGTGACCTCTGCCCTTGGGCTGCTTCCTCTTTTTGTGATTAATACATTTTTCGTTTCTTATAAAAAAAATAAAATAAAATCCATTGAGGTTGAAGTTGATCTATGATTCTAGCCTTCTAGGTGTAGGAGTTATAGAATACATTGTTAAGTTTTGGCATTCTAAGTTAAAGAAGCATCTCTTGTGAGTATATTCTAACATGTTCTTTATGCTAAAACTTCTTCATCAGTCAACTTCGCAACTTATTTTTGGCAGCCTATGTGAAGTTTCTTGATAATATTTTATTCGTTTCATTGATTTTGCCCCATGTACCAATTTTGGAGATTTTTTTAGGTCCTATATATATTCTTTTTCCACTGAGGTATTCTTTTTCCTGGTTCTTTCCACTGATCTTAGAAGAAGTTTTGTCCACCAAAGTTAGACCACAAGGCAAAAAGAGGTTTAAGTTATAAGGTCTCGTTTGATAGCGATTTAGTTTTTTGAAAATTATGTTGGTTTGCCTACTGTTACAGTTTTCATGGTATGTTTTCACATTTTTTAAAAATACATTTGAATAAAAAATCTAAAAACAAAAACAAAGTGTTCGAAAACTACTCTTTTTTTTAGTTTTAAAATATAGACATTTTTTTAAAAGCTAGGTAGCAAAATAAAGAAAGTACATGGGTGGAAGAAATGTTTACAAGCTTAATTTTTAAAAGTAGAAAATAAAAAATAAAATTTTTATCAAATAGAAAATTTCTTGTTTTCAGTGTTTTCGAAGGTGTGTTTGGTTCAAATTTGGAATTTGAATCTCAATTTAAAAAATTATTCAACGTGTGTTTAGTAATATCTATATCTATACTATATTAAAAAGGTAGGAGGTAGGAAAAACATTTTTGGACACTTTTGCCCTTTACTAAATTTACAAATTATGCCATTAATATATTATTACAAATATTAAATTAATTATTAATTATTAATTATTAATTATCAATACTATAAATGCATGGAATATAAAAGGTTAGAATGCAAAAAGTTTTCCAACAACATAAAATTAATTTTTTCTTTAATTAAAATTCATACTAGAATTTCAAGAGTTTTAGAATTTGAAAAGCTTTTGTAACGTTTTATCCTCTACTACTATAAAAATAGGCTTTCTTCTACATGTTTTTCACAAGTTTCAATACTTTTTTATTCTTAGTTCCTTGAAACATCTCTCAAAGATGATTCAATCTTGAAAACATCTCGAGATATTTGTGACATATTCATCAAGTTGCAAGAGAGGCATATGTTGAATCTACATATCTCGAATACTAGGTTTGTAACATTACCATTCCACAATTTTACAAAGTTGAATAATAGTATCTGTCAAGCAATCTTCAAGAATGAGCATATTTACTCTAAAAGATGAGTGTATAGTGAGTACTTGGATTTTTTTTGTTACTATTGTTATTTTTCTAATTAGTGTGTTTTTTATTTGGAATGTTTCTTTTTTTAGCAATTTTATTTAGTTTAGGATTTGGACTTTTTTTTTGTTACTAATGTTTTTTATTGTTTAGTGTATTTTTTTTAGCAATTTTATTTAGAATATTTGTTTAAAATATAGATAATTACAAGCTCATGTTCTTATTGTTGAAAAAAAAACAATCATAGAAGTCAGTATATAAAAAAATGTTTAATTAATAAATTAATTATAAACAATAATTTGTATAGAATCGTAAAAGGTGGAATATAAAACTTTTGGTCATTTTATTTTCTAATAATATAATTTGAAGAATTTTTCTTTAATAATTTTAATTATAATATAAAATTAAATCAATAAAATAATTTAGATTTAGTTAAAAAATTCAACATATACAACAAAATGTCAGCCCTAAACAAATATTTAATAAACAAACAAAATATTAAAAAAATAAAATAAAGGACCCATATTAGGTTGAAATAATTTAAAAATAAAAATATTAAAATACCTATTTACTTTTTTATTAATATATAATTTTTTCCTTTACTTTTAATTTATTTATTTACAAAATATATCATTAACAATTAATTATAAATAGTATATTTTAAAAATAATTATAAATAGTACATTTTATTATTAGTAATTAGCTGTCAATAATAATAAATATATATAAAAATTAATCTTAATTATTTTTTAAAATAATTATACTTAAAATATTTTACGTGAAATTATATAAAATTTAAGTTACCTTATAATTAAAAAAAATTTATACGTGCATTGCACGTGGTAATACACTAGTATATGTAAACTAGTGATAGTTATCAATTAAATATTTTATTCAATCGAAAATAATATTACTTAATTTATTAACCTTTTTGTTATAAATTTTGTGAAATTATTATCTTACAATATTATATAATTTATGATATATTATGTTTTACATGTTTTAATTTAAAAAAAATAAAACAAGTTTAAACATGGAATAGTATATCTATCAATGTTTTTATCTTTTTTAATGTAATCCTGTATTTTAAAGTTTCAAATTTAAAATCTGAAAACACTCAAAGACAAAAATGTTTGTTTTCAGAAATTTCCATGGTTTGGATCATAGAGAAAACCGTTTTAAAAAATAAATCTGCCGAGGCCCTAAGTTTTCAACTATGAGTTTGACTTTAGGACCTGCATGACATCTCTAAAGTTAGCTCCAAAAGATAGAAAGTGATTTAAATTATTAACTTTCCAAGTAGAGAGCCTGAATCTAGGACCTCGTAGAATATATAATTCTTACCATTGCAGCACGTCTTAGGGTATCATTACTATCACTTCGTTATACAAATCACATCGAGCATTTTGTCGCCCTTACTGTCTCACCTATCTGTTTGGTGTGTGGATACGTATTTGCATTTCTTGTGCCAAATCAGTCAAAATCTTATGTGATTTTGCATGTTTTGTGTTAGCTACCGCGCATACAGGTGACCGACCCGGTTGCAAGATATTACGGGCTAAAGCGTGGGCAAGTTGTGAAGATCATCAGGCCAAGTGAGACGGCAGGAAGATATGTAACTTACAGATACGTTGTT

mRNA sequence

GTGCTATCAGAGGAGGAAATTACCAGGCTTTTCAGAATTCGAAAAACTGTGTTACAAATGCTAAAAGACCGAAGCTATTTTGTTGGAGACTTTGAGATTGACATGTCAAGAGAGCAATTTAAGCATAAATATGGTGAAAACATGAAAAGAGAAGATATTGTTATCAATAAAGCCAGGAGAAATGATAGCTCTGATCAGATATATGTTTTCTTTCCAGAAGAACAGAAGGTTGGCGTAAAGACGATGAAAACTTATACCAATCGCATGAAATCGGAGAATGTCTTTAGAGCAATATTGGTCGTTCAACAAAATTTGACTCCCTTCGCTCGTACTTGTATAAGTGAAATATCTTCTAAATTCCACCTGGAAGTTTTCCAGGAAGCTGAATTGCTAGTTAACATTAAAGATCACGTTCTTGTTCCCGAGCACCAGTTACTTACCAACGAAGAGAAGAAGACCCTGCTGGAGAGATATACCGTGAAAGAAACACAGCTACCGCGCATACAGGTGACCGACCCGGTTGCAAGATATTACGGGCTAAAGCGTGGGCAAGTTGTGAAGATCATCAGGCCAAGTGAGACGGCAGGAAGATATGTAACTTACAGATACGTTGTT

Coding sequence (CDS)

GTGCTATCAGAGGAGGAAATTACCAGGCTTTTCAGAATTCGAAAAACTGTGTTACAAATGCTAAAAGACCGAAGCTATTTTGTTGGAGACTTTGAGATTGACATGTCAAGAGAGCAATTTAAGCATAAATATGGTGAAAACATGAAAAGAGAAGATATTGTTATCAATAAAGCCAGGAGAAATGATAGCTCTGATCAGATATATGTTTTCTTTCCAGAAGAACAGAAGGTTGGCGTAAAGACGATGAAAACTTATACCAATCGCATGAAATCGGAGAATGTCTTTAGAGCAATATTGGTCGTTCAACAAAATTTGACTCCCTTCGCTCGTACTTGTATAAGTGAAATATCTTCTAAATTCCACCTGGAAGTTTTCCAGGAAGCTGAATTGCTAGTTAACATTAAAGATCACGTTCTTGTTCCCGAGCACCAGTTACTTACCAACGAAGAGAAGAAGACCCTGCTGGAGAGATATACCGTGAAAGAAACACAGCTACCGCGCATACAGGTGACCGACCCGGTTGCAAGATATTACGGGCTAAAGCGTGGGCAAGTTGTGAAGATCATCAGGCCAAGTGAGACGGCAGGAAGATATGTAACTTACAGATACGTTGTT

Protein sequence

VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARRNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV

Homology

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BLAST of MS020723 vs. NCBI nr
Match: XP_022143609.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia])

HSP 1 Score: 397.1 bits (1019), Expect = 9.0e-107
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

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BLAST of MS020723 vs. NCBI nr
Match: XP_022991826.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023547519.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 391.0 bits (1003), Expect = 6.5e-105
Identity = 200/205 (97.56%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDR YFVGDFEIDMSREQFKHKYGE+MKREDIVINKA+R
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVN+KDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. NCBI nr
Match: XP_022953770.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata])

HSP 1 Score: 390.6 bits (1002), Expect = 8.5e-105
Identity = 199/205 (97.07%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDR YFVGDFEIDMSREQFKHKYGE+MKRED+VINKA+R
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDVVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVN+KDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.5 bits (981), Expect = 2.3e-102
Identity = 194/205 (94.63%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VL+EEEITRLFR+RKTVLQMLKDR YFVGDFE+DMSREQFK+KYGE+MKREDIVINKA+R
Sbjct: 2   VLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIY+FFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+KF
Sbjct: 62  NDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVNIKDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. NCBI nr
Match: XP_022142704.1 (DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia] >XP_022142705.1 DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia])

HSP 1 Score: 377.1 bits (967), Expect = 9.7e-101
Identity = 190/205 (92.68%), Postives = 201/205 (98.05%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VL++EEITRLFRIRKTVLQMLKDR YFVGDFEI MSREQF++KYGE+MKRED+VINKA+R
Sbjct: 2   VLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIY+FFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCI EISSKF
Sbjct: 62  NDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAEL+VNIKDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYGL
Sbjct: 122 HLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

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BLAST of MS020723 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 2.9e-92
Identity = 167/205 (81.46%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           +L+EEE+ RL+RI+KT++QML+DR YF+ D E+ M+++QF  K+G+NMKRED+V  KA+R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           ND+SDQ+Y+FFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAE+LVNIK+HVLVPEHQ+LT EEKKTLLERYTVKETQLPRIQVTDP+ARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 KRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of MS020723 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-56
Identity = 110/206 (53.40%), Postives = 152/206 (73.79%), Query Frame = 0


Query: 4   EEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGE-----NMKREDIVINKA 63
           EEE  RL++IRKT++Q+  DR Y V   E+D + E+FK ++G+       +R D+ +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 64  RRNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 123
             +D +DQ++VFFPEE KVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 124 KFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 183
           K+ LE F + ELL+NI +H LVPEH ++T EE   LL RY ++E+QLPRIQ  DPVARY+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYF 183

Query: 184 GLKRGQVVKIIRPSETAGRYVTYRYV 205
           G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 GIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of MS020723 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-56
Identity = 110/206 (53.40%), Postives = 152/206 (73.79%), Query Frame = 0


Query: 4   EEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGE-----NMKREDIVINKA 63
           EEE  RL++IRKT++Q+  DR Y V   E+D + E+FK ++G+       +R D+ +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 64  RRNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 123
             +D +DQ++VFFPEE KVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 124 KFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 183
           K+ LE F + ELL+NI +H LVPEH ++T EE   LL RY ++E+QLPRIQ  DPVARY+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYF 183

Query: 184 GLKRGQVVKIIRPSETAGRYVTYRYV 205
           G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 GIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of MS020723 vs. ExPASy Swiss-Prot
Match: Q9N5K2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans OX=6239 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.8e-56
Identity = 115/207 (55.56%), Postives = 150/207 (72.46%), Query Frame = 0


Query: 4   EEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMK-----REDIVINKA 63
           E E  RL+RIRKTVLQM+ DR Y V   E+D   E FK +YG+        R D+ I  A
Sbjct: 5   ELETYRLWRIRKTVLQMVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLTILVA 64

Query: 64  RRNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 123
             +D +DQ++VFFPE+ K+G+KT+K    +M+ +N+ RAI+VVQ  +TP A+  I +++ 
Sbjct: 65  HNDDPADQMFVFFPEDAKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDMAP 124

Query: 124 KFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 183
           K+ LE F EAEL+VNI +H LVPEH ++T EEK  LL RY +K++QLPRIQ  DPVARY+
Sbjct: 125 KYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVARYF 184

Query: 184 GLKRGQVVKIIRPSETAGRYVTYRYVV 206
           GL+RGQVVKIIRPSETAGRY+TYR VV
Sbjct: 185 GLRRGQVVKIIRPSETAGRYITYRLVV 211

[Close]

BLAST of MS020723 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 7.5e-56
Identity = 109/206 (52.91%), Postives = 151/206 (73.30%), Query Frame = 0


Query: 4   EEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGE-----NMKREDIVINKA 63
           EEE  RL++IRKT++Q+  DR Y V   E+D + E+F+ ++G+       +R D+ +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 64  RRNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 123
             +D +DQ++VFFPEE KVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 124 KFHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYY 183
           K+ LE F + ELL+NI +H LVPEH ++T EE   LL RY ++E QLPRIQ  DPVARY+
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYF 183

Query: 184 GLKRGQVVKIIRPSETAGRYVTYRYV 205
           G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 GIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of MS020723 vs. ExPASy TrEMBL
Match: A0A6J1CPU1 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX=3673 GN=LOC111013465 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.4e-107
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. ExPASy TrEMBL
Match: A0A6J1JRV4 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111488357 PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.1e-105
Identity = 200/205 (97.56%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDR YFVGDFEIDMSREQFKHKYGE+MKREDIVINKA+R
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVN+KDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. ExPASy TrEMBL
Match: A0A6J1GQK9 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111456196 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.1e-105
Identity = 199/205 (97.07%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VLSEEEITRLFRIRKTVLQMLKDR YFVGDFEIDMSREQFKHKYGE+MKRED+VINKA+R
Sbjct: 2   VLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDVVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIYVFFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 62  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVN+KDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.1e-102
Identity = 194/205 (94.63%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VL+EEEITRLFR+RKTVLQMLKDR YFVGDFE+DMSREQFK+KYGE+MKREDIVINKA+R
Sbjct: 2   VLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIY+FFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+KF
Sbjct: 62  NDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVNIKDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. ExPASy TrEMBL
Match: A0A6J1H699 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111460591 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.1e-102
Identity = 194/205 (94.63%), Postives = 203/205 (99.02%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           VL+EEEITRLFR+RKTVLQMLKDR YFVGDFE+DMSREQFK+KYGE+MKREDIVINKA+R
Sbjct: 2   VLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKR 61

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           NDSSDQIY+FFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+KF
Sbjct: 62  NDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTKF 121

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAELLVNIKDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYGL
Sbjct: 122 HLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 182 KRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MS020723 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 339.3 bits (869), Expect = 2.1e-93
Identity = 167/205 (81.46%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 1   VLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARR 60
           +L+EEE+ RL+RI+KT++QML+DR YF+ D E+ M+++QF  K+G+NMKRED+V  KA+R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 61  NDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120
           ND+SDQ+Y+FFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 121 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 180
           HLEVFQEAE+LVNIK+HVLVPEHQ+LT EEKKTLLERYTVKETQLPRIQVTDP+ARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

Query: 181 KRGQVVKIIRPSETAGRYVTYRYVV 206
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 KRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of MS020723 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 176.8 bits (447), Expect = 1.8e-44
Identity = 94/206 (45.63%), Postives = 143/206 (69.42%), Query Frame = 0


Query: 4   EEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENM---KREDIVINKARR 63
           ++EITR+F++R+TVLQML+DR Y + + ++++ RE+F  ++ + M    +E + ++  + 
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 64  NDSSDQIYVFFPEEQKVGVKTM-KTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 123
            + +D+IYVF+PE  KVGV  + K    +M+ + V R I+VV   +T  AR  +SE++  
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKM 124

Query: 124 FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 183
             +EVF+EAEL+ NI +H LV ++ +L ++ KK LL  YTV++TQLPRI VTDP+ARYYG
Sbjct: 125 LTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYYG 184

Query: 184 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKI R   T+  Y TYR+ V
Sbjct: 185 LKRGQVVKIRRSDATSLDYYTYRFAV 210

[Close]

BLAST of MS020723 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 133.7 bits (335), Expect = 1.7e-31
Identity = 78/203 (38.42%), Postives = 126/203 (62.07%), Query Frame = 0


Query: 3   SEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARRND 62
           S  E  + +  R+T ++ML+DR Y V D +I++S +QF+  YGE+   + + I+   R D
Sbjct: 17  SSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFD 76

Query: 63  SSDQIYVFFPEEQKVGVKTMKTY-TNRMKSENVFRAILVVQQNLTPFARTCISEISSKFH 122
           SS +I V F     V V  M+    + +  EN+   ILV+Q ++T  A   +   S  F 
Sbjct: 77  SSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFS--FK 136

Query: 123 LEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLK 182
           +E+F+  +LLVN+  HVL P+HQ+L ++EK++LL++++++E QLPR+   DP+ RYYGL+
Sbjct: 137 VELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGLE 196

Query: 183 RGQVVKIIRPSETAGRYVTYRYV 205
            GQV+K+    E +  +VTYR V
Sbjct: 197 TGQVMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of MS020723 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-30
Identity = 79/204 (38.73%), Postives = 121/204 (59.31%), Query Frame = 0


Query: 2   LSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARRN 61
           LS EE  R +  R+  LQML+DR Y V D +I++S   F+  YGE    + + I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 62  DSSDQIYVFFPEEQKVGVKTMKT-YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 121
           DS+ ++ + F     V V  +++   + +  E +   ILV+Q ++T  A   I   S  F
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFS--F 139

Query: 122 HLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 181
            +E+FQ  +LLVNI  H L P+HQ+L +EEK TLL++++++E QLPRI   D + RYYGL
Sbjct: 140 KVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGL 199

Query: 182 KRGQVVKIIRPSETAGRYVTYRYV 205
           ++GQVVK+    E    +V +R V
Sbjct: 200 EKGQVVKVNYRGELTESHVAFRCV 221

[Close]

BLAST of MS020723 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 112.1 bits (279), Expect = 5.4e-25
Identity = 67/201 (33.33%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 6   EITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKARRNDSSD 65
           E  R +  R T  +ML+DR Y V + E+ ++  +F+  +GE  + E + I    R+D   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 66  QIYVFFPEEQKVGVKTMKTYTNRMKSE-NVFRAILVVQQNLTPFARTCISEISSKFHLEV 125
           +I V F   + + VK+++    ++ +   +   ILV+Q  +  FA+  ++  +  F +E 
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKALT--TFPFTVET 154

Query: 126 FQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQ 185
           F   +LLVNI  H+  P+ ++L  EEK+ LL ++ +++ QLP +Q  D   RYYGLK+ Q
Sbjct: 155 FPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKKQ 214

Query: 186 VVKIIRPSETAGRYVTYRYVV 206
           VVKI    E  G +VTYR ++
Sbjct: 215 VVKITYSKEPVGDFVTYRCII 233

The following BLAST results are available for this feature:

BLAST of MS020723 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143609.1	9.0e-107	100.00	DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia]	[more]
XP_022991826.1	6.5e-105	97.56	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_02...	[more]
XP_022953770.1	8.5e-105	97.07	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata]	[more]
XP_022959553.1	2.3e-102	94.63	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]
XP_022142704.1	9.7e-101	92.68	DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia] >XP_0221...	[more]

BLAST of MS020723 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	2.9e-92	81.46	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	2.6e-56	53.40	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	2.6e-56	53.40	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q9N5K2	5.8e-56	55.56	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis ele...	[more]
Q5R587	7.5e-56	52.91	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]

BLAST of MS020723 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPU1	4.4e-107	100.00	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX...	[more]
A0A6J1JRV4	3.1e-105	97.56	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1GQK9	4.1e-105	97.07	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KT45	1.1e-102	94.63	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H699	1.1e-102	94.63	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]

BLAST of MS020723 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	2.1e-93	81.46	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	1.8e-44	45.63	RNA polymerase II fifth largest subunit, C	[more]
AT2G41340.1	1.7e-31	38.42	RNA polymerase II fifth largest subunit, D	[more]
AT3G57080.1	1.9e-30	38.73	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT3G54490.1	5.4e-25	33.33	RNA polymerase II fifth largest subunit, E	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 132..204 e-value: 8.0E-35 score: 118.6
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 130..205 e-value: 5.9E-35 score: 120.8
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 130..205
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 2..129 e-value: 3.0E-47 score: 162.1
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 4..133
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 1..205 e-value: 2.0E-90 score: 299.8
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 4..89 e-value: 1.1E-31 score: 109.3
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..205
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..205
IPR020608	RNA polymerase, subunit H/Rpb5, conserved site	PROSITE	PS01110	RNA_POL_H_23KD	coord: 137..150
IPR020609	Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit	HAMAP	MF_00025	RNApol_RpoH_RPB5	coord: 132..205 score: 22.839989

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020723.1	MS020723.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity