MS020687 (gene) Bitter gourd (TR) v1

Overview


Name	MS020687
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold375: 2215626 .. 2216228 (-)
RNA-Seq Expression	MS020687
Synteny	MS020687

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCGAGTCCGCCGCCGCCACCTCCGGTGCCTGATTACTCTGGCTGGAGTCCGTTGTCAATCTCGACCATCGTCGTCGTCTGCATCATCTTCCTGTTGTTGAGCAACTACAGAATATTGAAACGATTGTGCCATGTCCTTCATGGGGTGATTTTCTGCCGACCTCGGGCACACTTTGAAGAAAACCCTAACGATGCTTCTCCGCAAATCCATGGCGATGGATTGGATGCCTCGATGATTCGTTTGCTACCGATCTCACAGTTCAAAAAGAACGTAGAAGCCGGATCCCCGGCAAGTATCAATACGGAATGCGCGGTTTGCTTGGGCGATTTTGAAGAAGGGGAACTGATTAAACATTTACCAAATTGCAATCACTCGTTTCATAGCTCTTGCATCGATTCCTGGTTCCGAAACCACTCGAGTTGCCCTCTCTGCAGATCGCATGTCTTGGATTTCTCCGCCGATTTTACCAGTCCCAATTCTGCGTCGGCAATGCTGGAGACGCTGGGAAGGGAAGACATTTTACGAGAAGGGGATTATCAAACTATTCGTGCTCAAATTCTGCAAAGCTCGGAATTGAGAAGAGATGCATCGGATCAA

mRNA sequence

ATGCCATCGAGTCCGCCGCCGCCACCTCCGGTGCCTGATTACTCTGGCTGGAGTCCGTTGTCAATCTCGACCATCGTCGTCGTCTGCATCATCTTCCTGTTGTTGAGCAACTACAGAATATTGAAACGATTGTGCCATGTCCTTCATGGGGTGATTTTCTGCCGACCTCGGGCACACTTTGAAGAAAACCCTAACGATGCTTCTCCGCAAATCCATGGCGATGGATTGGATGCCTCGATGATTCGTTTGCTACCGATCTCACAGTTCAAAAAGAACGTAGAAGCCGGATCCCCGGCAAGTATCAATACGGAATGCGCGGTTTGCTTGGGCGATTTTGAAGAAGGGGAACTGATTAAACATTTACCAAATTGCAATCACTCGTTTCATAGCTCTTGCATCGATTCCTGGTTCCGAAACCACTCGAGTTGCCCTCTCTGCAGATCGCATGTCTTGGATTTCTCCGCCGATTTTACCAGTCCCAATTCTGCGTCGGCAATGCTGGAGACGCTGGGAAGGGAAGACATTTTACGAGAAGGGGATTATCAAACTATTCGTGCTCAAATTCTGCAAAGCTCGGAATTGAGAAGAGATGCATCGGATCAA

Coding sequence (CDS)

ATGCCATCGAGTCCGCCGCCGCCACCTCCGGTGCCTGATTACTCTGGCTGGAGTCCGTTGTCAATCTCGACCATCGTCGTCGTCTGCATCATCTTCCTGTTGTTGAGCAACTACAGAATATTGAAACGATTGTGCCATGTCCTTCATGGGGTGATTTTCTGCCGACCTCGGGCACACTTTGAAGAAAACCCTAACGATGCTTCTCCGCAAATCCATGGCGATGGATTGGATGCCTCGATGATTCGTTTGCTACCGATCTCACAGTTCAAAAAGAACGTAGAAGCCGGATCCCCGGCAAGTATCAATACGGAATGCGCGGTTTGCTTGGGCGATTTTGAAGAAGGGGAACTGATTAAACATTTACCAAATTGCAATCACTCGTTTCATAGCTCTTGCATCGATTCCTGGTTCCGAAACCACTCGAGTTGCCCTCTCTGCAGATCGCATGTCTTGGATTTCTCCGCCGATTTTACCAGTCCCAATTCTGCGTCGGCAATGCTGGAGACGCTGGGAAGGGAAGACATTTTACGAGAAGGGGATTATCAAACTATTCGTGCTCAAATTCTGCAAAGCTCGGAATTGAGAAGAGATGCATCGGATCAA

Protein sequence

MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILREGDYQTIRAQILQSSELRRDASDQ

Homology

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BLAST of MS020687 vs. NCBI nr
Match: XP_031743630.1 (RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical protein Csa_023057 [Cucumis sativus])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 153/203 (75.37%), Postives = 167/203 (82.27%), Query Frame = 0


Query: 1   MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHF 60
           MPSSPPPP PVPDYS WSP SISTIVVVCI+FLLLSNYR+LK+LC VLHG+   RP   F
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKH 120
           EENPN+ S QIHG GL+ SMIRLLPISQFKKN E+ S  S NTECAVCLG+FEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE-- 180
           LPNCNHSFHS CID+WFRNHSSCPLCR  VL FS   T  +S+SAMLETLGRE++LRE  
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFS---TPDSSSSAMLETLGRENVLRERV 180

Query: 181 GDYQTIRAQILQSSELRRDASDQ 202
             YQT+RAQILQ  E RRDAS Q
Sbjct: 181 AHYQTLRAQILQHPEFRRDASIQ 200

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BLAST of MS020687 vs. NCBI nr
Match: KAG6570731.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010575.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 294.3 bits (752), Expect = 8.1e-76
Identity = 151/206 (73.30%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MPSSPPPPP---PVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPR 60
           MPSSPPPPP   PVPDYS WSP SIS I +VCI+ LLLSNYRILKR C VLHG+   R  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHGLFCGRFV 60

Query: 61  AHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGEL 120
             F+ENPND SP++H  GL+AS IRLLPISQFKKN EA S ASINTECAVCLG+FEEGEL
Sbjct: 61  VQFDENPNDPSPEVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILR 180
           IK+LPNCNHSFHSSCID+W RNHSSCPLCR HV + S D  +P+S+SAMLETLGRED+ R
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLGREDVER 180

Query: 181 E--GDYQTIRAQILQSSELRRDASDQ 202
           E    YQT+RAQILQ+ ELRRDAS Q
Sbjct: 181 ERVSYYQTLRAQILQNPELRRDASVQ 206

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BLAST of MS020687 vs. NCBI nr
Match: XP_023512261.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 3.4e-74
Identity = 148/206 (71.84%), Postives = 165/206 (80.10%), Query Frame = 0


Query: 1   MPSSPPPPP---PVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPR 60
           MPSSPPPPP   PVPDYS WSP SIS I +VCI+ LLLSNYRILKR C V HG+   R  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVFHGLFCGRSV 60

Query: 61  AHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGEL 120
             F+ENPND SPQ+H  GL+AS IRLLPISQFKKN EA S ASINTECAVCLG+FEEGEL
Sbjct: 61  VQFDENPNDPSPQVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDI-- 180
           IK+LPNCNHSFHSSCID+W RNHSSCPLCR HV + S D  +P+S+SAMLETL RED+  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLSREDVDG 180

Query: 181 LREGDYQTIRAQILQSSELRRDASDQ 202
            R   YQT+RAQILQ+ +LRRDAS Q
Sbjct: 181 ERVSYYQTLRAQILQNPDLRRDASVQ 206

[Close]

BLAST of MS020687 vs. NCBI nr
Match: XP_008448367.1 (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa] >TYK23976.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa])

HSP 1 Score: 287.7 bits (735), Expect = 7.6e-74
Identity = 147/203 (72.41%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHF 60
           MPSSPPPP PVPDYS WSP SISTIV+VC+IFLLLSNYR+LK+LC VLH +   RP   F
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKH 120
           EENPN+ S QIH  GL+ SMIRLLPISQFKK  E+ S  S NTECAVCLG+FEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE-- 180
           LPNCNHSFHSSCID+WFR+HSSCPLCR  V  FS    +P+S+SAMLETLGRE+ILRE  
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 GDYQTIRAQILQSSELRRDASDQ 202
             YQT+RAQILQ  E RRD S Q
Sbjct: 181 AHYQTLRAQILQHPEFRRDESIQ 199

[Close]

BLAST of MS020687 vs. NCBI nr
Match: XP_034689191.1 (RING-H2 finger protein ATL52-like [Vitis riparia])

HSP 1 Score: 174.5 bits (441), Expect = 9.3e-40
Identity = 94/195 (48.21%), Postives = 123/195 (63.08%), Query Frame = 0


Query: 8   PPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIF---CRPRAHFEENP 67
           P P   +  WS   I  IV      LL + YRI+K++C+  HG  F    + +   E NP
Sbjct: 7   PYPNSKFPIWSLPLICLIVTAFASILLFTCYRIVKQICYGHHGESFRNWVQRQLLDEANP 66

Query: 68  NDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKHLPNC 127
           +D S Q H  GLD+S +  LPI+QFKKN     P+  NT+CAVCLG+FEEGE +KHLPNC
Sbjct: 67  DDPSQQFHSRGLDSSTVYSLPIAQFKKN---EGPSHSNTDCAVCLGEFEEGEFLKHLPNC 126

Query: 128 NHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE--GDYQ 187
           +H FH  CID+WF +HS+CPLCRSHV DF+ D     S   +LETL RED  +E   +YQ
Sbjct: 127 SHVFHIPCIDTWFESHSNCPLCRSHVYDFTMDNEFSGSMYTLLETLRREDFFQEWVENYQ 186

Query: 188 TIRAQILQSSELRRD 198
            +R +ILQ+S  R++
Sbjct: 187 ILRVEILQTSTHRQE 198

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BLAST of MS020687 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.7e-20
Identity = 67/180 (37.22%), Postives = 97/180 (53.89%), Query Frame = 0


Query: 6   PPPPPVPDYSGWSP--------------LSISTIVVVCIIFLLLSNYRILKRLCHVLHGV 65
           PPPPP P  +  SP              L+I+ I ++   FLL+S Y  + + C   H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -IFCRPRAHFEENP-NDASPQIHGDGLDASMIRLLPISQFKK-NVEAG--SPASINTECA 125
            IF R R   ++NP    SP     GLD S IR +P+ +FKK +V AG    +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHV---LDFSADFTSPNSA 164
           VCL +F+E E ++ +PNC H FH  CID W + +++CPLCR+ V     F+ D  S  S+
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

[Close]

BLAST of MS020687 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.9e-19
Identity = 56/157 (35.67%), Postives = 88/157 (56.05%), Query Frame = 0


Query: 5   PPPPPPV-------PDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRP- 64
           PPPPP            S +SPL I+ I ++    +L+S Y ++ + CH  H        
Sbjct: 35  PPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETL 94

Query: 65  -RAHFEENPNDASPQI--HGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFE 124
              H  E    ++ +I  +GDGL+ SMI+ + + ++K    +G      ++C+VCL +FE
Sbjct: 95  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYK----SGDGFVDGSDCSVCLSEFE 154

Query: 125 EGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHV 151
           E E ++ LP CNH+FH  CID+W ++HS+CPLCR+ V
Sbjct: 155 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 187

[Close]

BLAST of MS020687 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.2e-19
Identity = 62/215 (28.84%), Postives = 107/215 (49.77%), Query Frame = 0


Query: 5   PPPPPPV-----PDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLC------HVLHGVIF 64
           PPPPPP+        + +  L+++ I ++   FLL+S Y  + + C       +L     
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  CRPRAHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAG----------SPASINT 124
            R R + ++     SP++   GLD S+IR +PI +FKK  +                 + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSA 184
           EC+VCL +F++ E ++ +PNC+H FH  CID W +N+++CPLCR+ V   S D + P   
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV---SCDTSFPPDR 196

Query: 185 SAMLETLGREDILREGDYQTIRAQILQSSELRRDA 199
            +   T     ++  G+ + +  ++  S    RD+
Sbjct: 197 VSAPSTSPENLVMLRGENEYVVIELGSSIGSDRDS 228

[Close]

BLAST of MS020687 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 9.5e-19
Identity = 59/182 (32.42%), Postives = 90/182 (49.45%), Query Frame = 0


Query: 5   PPPPP------PVPDYSG--WSPLSISTIVVVCIIFLLLSNYRILKRLCHV---LHGVIF 64
           PPPPP         D SG  +SPL I+ I V+   FLL+S Y  + + C     L G +F
Sbjct: 35  PPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAFLLVSYYTFISKYCGTVSSLRGRVF 94

Query: 65  CRPRAHFEENPNDASPQIHG---------------DGLDASMIRLLPISQFKKNVEAGSP 124
                         S   HG                GLD ++I  + + ++++    G  
Sbjct: 95  GSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVSPPSGLDETLINKITVCKYRR----GDG 154

Query: 125 ASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFT 161
               T+C+VCLG+F +GE ++ LP C+H+FH  CID+W ++HS+CPLCR+++   +    
Sbjct: 155 FVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCRANITFVTVGLA 212

[Close]

BLAST of MS020687 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 4.7e-18
Identity = 58/179 (32.40%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 5   PPPPPPVPD------YSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRA 64
           PPPP    D       S +SPL I+ I ++   F+L+S Y ++ + CH           A
Sbjct: 38  PPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAA 97

Query: 65  HFEE-------------------NPNDASPQIHGDGLDASMIRLLPISQFKKN---VEAG 124
                                  NPN       GDGLD S+I+ + + +++K    VE  
Sbjct: 98  AINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVE-- 157

Query: 125 SPASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSA 156
                +++C+VCL +F+E E ++ LP CNH+FH  CID+W ++HS+CPLCR+ ++  SA
Sbjct: 158 -----SSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSA 209

[Close]

BLAST of MS020687 vs. ExPASy TrEMBL
Match: A0A0A0KCA5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.5e-78
Identity = 153/203 (75.37%), Postives = 167/203 (82.27%), Query Frame = 0


Query: 1   MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHF 60
           MPSSPPPP PVPDYS WSP SISTIVVVCI+FLLLSNYR+LK+LC VLHG+   RP   F
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKH 120
           EENPN+ S QIHG GL+ SMIRLLPISQFKKN E+ S  S NTECAVCLG+FEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE-- 180
           LPNCNHSFHS CID+WFRNHSSCPLCR  VL FS   T  +S+SAMLETLGRE++LRE  
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFS---TPDSSSSAMLETLGRENVLRERV 180

Query: 181 GDYQTIRAQILQSSELRRDASDQ 202
             YQT+RAQILQ  E RRDAS Q
Sbjct: 181 AHYQTLRAQILQHPEFRRDASIQ 200

[Close]

BLAST of MS020687 vs. ExPASy TrEMBL
Match: A0A5A7V3S2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00390 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.7e-74
Identity = 147/203 (72.41%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHF 60
           MPSSPPPP PVPDYS WSP SISTIV+VC+IFLLLSNYR+LK+LC VLH +   RP   F
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKH 120
           EENPN+ S QIH  GL+ SMIRLLPISQFKK  E+ S  S NTECAVCLG+FEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE-- 180
           LPNCNHSFHSSCID+WFR+HSSCPLCR  V  FS    +P+S+SAMLETLGRE+ILRE  
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 GDYQTIRAQILQSSELRRDASDQ 202
             YQT+RAQILQ  E RRD S Q
Sbjct: 181 AHYQTLRAQILQHPEFRRDESIQ 199

[Close]

BLAST of MS020687 vs. ExPASy TrEMBL
Match: A0A1S3BIX6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.7e-74
Identity = 147/203 (72.41%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MPSSPPPPPPVPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHF 60
           MPSSPPPP PVPDYS WSP SISTIV+VC+IFLLLSNYR+LK+LC VLH +   RP   F
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKH 120
           EENPN+ S QIH  GL+ SMIRLLPISQFKK  E+ S  S NTECAVCLG+FEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGREDILRE-- 180
           LPNCNHSFHSSCID+WFR+HSSCPLCR  V  FS    +P+S+SAMLETLGRE+ILRE  
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 GDYQTIRAQILQSSELRRDASDQ 202
             YQT+RAQILQ  E RRD S Q
Sbjct: 181 AHYQTLRAQILQHPEFRRDESIQ 199

[Close]

BLAST of MS020687 vs. ExPASy TrEMBL
Match: A0A438G1M9 (E3 ubiquitin-protein ligase OS=Vitis vinifera OX=29760 GN=Os04g0590900 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 7.2e-38
Identity = 84/164 (51.22%), Postives = 111/164 (67.68%), Query Frame = 0


Query: 39  RILKRLCHVLHGVIF---CRPRAHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEA 98
           RI+K++C+  +G  F      +   E NP+D S Q H  GLD+S + LLPI+QFKKN   
Sbjct: 390 RIVKQICYGHYGESFRDWVERQLLDEANPDDPSQQFHSRGLDSSTVYLLPIAQFKKN--- 449

Query: 99  GSPASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSA 158
             P+  NT+CAVCLG+FEEGE +KHLPNC+H FH  CID+WF +HS+CPLCRSHV DF+ 
Sbjct: 450 EGPSHSNTDCAVCLGEFEEGEFLKHLPNCSHVFHIPCIDTWFESHSNCPLCRSHVYDFTM 509

Query: 159 DFTSPNSASAMLETLGREDILRE--GDYQTIRAQILQSSELRRD 198
           D     S   +LETL RED  +E   +YQ +R +ILQ+S  R++
Sbjct: 510 DNEFSGSMYTLLETLRREDFFQEWVENYQILRVEILQTSTHRQE 550

[Close]

BLAST of MS020687 vs. ExPASy TrEMBL
Match: A0A2C9W1B6 (RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G030200 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 2.1e-37
Identity = 95/200 (47.50%), Postives = 127/200 (63.50%), Query Frame = 0


Query: 1   MPSSPPPPPP--VPDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRA 60
           MP SP P  P  + +YS W+   I  ++ VC   +L S YR+L+RLC  L+     R RA
Sbjct: 1   MPPSPTPEAPLRLENYSQWNAYVIGPVIAVCSSVVLFSYYRMLRRLCCTLNTQTLPRNRA 60

Query: 61  HF----EENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFEE 120
                 + N  D+S Q +  GL+++++R LPISQFKK  E  S AS N ECAVCLG+FEE
Sbjct: 61  QMSRINQNNLEDSSLQNYSHGLESNIMRSLPISQFKKEEE--SEAS-NRECAVCLGEFEE 120

Query: 121 GELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSASAMLETLGRED 180
           GE +KHLPNC H FH +CID+WF+ HS+CPLCRSHV D   +++   S   +L TL RED
Sbjct: 121 GEWLKHLPNCAHVFHVACIDTWFQTHSNCPLCRSHVYDLCHEYSI--SMDTLLHTLRRED 180

Query: 181 ILRE--GDYQTIRAQILQSS 193
              E    YQ +R++IL++S
Sbjct: 181 FFNERAEHYQNLRSEILRNS 195

[Close]

BLAST of MS020687 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-21
Identity = 67/180 (37.22%), Postives = 97/180 (53.89%), Query Frame = 0


Query: 6   PPPPPVPDYSGWSP--------------LSISTIVVVCIIFLLLSNYRILKRLCHVLHGV 65
           PPPPP P  +  SP              L+I+ I ++   FLL+S Y  + + C   H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -IFCRPRAHFEENP-NDASPQIHGDGLDASMIRLLPISQFKK-NVEAG--SPASINTECA 125
            IF R R   ++NP    SP     GLD S IR +P+ +FKK +V AG    +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHV---LDFSADFTSPNSA 164
           VCL +F+E E ++ +PNC H FH  CID W + +++CPLCR+ V     F+ D  S  S+
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

[Close]

BLAST of MS020687 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.4e-20
Identity = 56/157 (35.67%), Postives = 88/157 (56.05%), Query Frame = 0


Query: 5   PPPPPPV-------PDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRP- 64
           PPPPP            S +SPL I+ I ++    +L+S Y ++ + CH  H        
Sbjct: 35  PPPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETL 94

Query: 65  -RAHFEENPNDASPQI--HGDGLDASMIRLLPISQFKKNVEAGSPASINTECAVCLGDFE 124
              H  E    ++ +I  +GDGL+ SMI+ + + ++K    +G      ++C+VCL +FE
Sbjct: 95  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYK----SGDGFVDGSDCSVCLSEFE 154

Query: 125 EGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHV 151
           E E ++ LP CNH+FH  CID+W ++HS+CPLCR+ V
Sbjct: 155 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 187

[Close]

BLAST of MS020687 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-20
Identity = 62/215 (28.84%), Postives = 107/215 (49.77%), Query Frame = 0


Query: 5   PPPPPPV-----PDYSGWSPLSISTIVVVCIIFLLLSNYRILKRLC------HVLHGVIF 64
           PPPPPP+        + +  L+++ I ++   FLL+S Y  + + C       +L     
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  CRPRAHFEENPNDASPQIHGDGLDASMIRLLPISQFKKNVEAG----------SPASINT 124
            R R + ++     SP++   GLD S+IR +PI +FKK  +                 + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSADFTSPNSA 184
           EC+VCL +F++ E ++ +PNC+H FH  CID W +N+++CPLCR+ V   S D + P   
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV---SCDTSFPPDR 196

Query: 185 SAMLETLGREDILREGDYQTIRAQILQSSELRRDA 199
            +   T     ++  G+ + +  ++  S    RD+
Sbjct: 197 VSAPSTSPENLVMLRGENEYVVIELGSSIGSDRDS 228

[Close]

BLAST of MS020687 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.3e-19
Identity = 58/179 (32.40%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 5   PPPPPPVPD------YSGWSPLSISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRA 64
           PPPP    D       S +SPL I+ I ++   F+L+S Y ++ + CH           A
Sbjct: 38  PPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAA 97

Query: 65  HFEE-------------------NPNDASPQIHGDGLDASMIRLLPISQFKKN---VEAG 124
                                  NPN       GDGLD S+I+ + + +++K    VE  
Sbjct: 98  AINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVE-- 157

Query: 125 SPASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNHSSCPLCRSHVLDFSA 156
                +++C+VCL +F+E E ++ LP CNH+FH  CID+W ++HS+CPLCR+ ++  SA
Sbjct: 158 -----SSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSA 209

[Close]

BLAST of MS020687 vs. TAIR 10
Match: AT5G05280.1 (RING/U-box superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.2e-18
Identity = 46/132 (34.85%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 21  SISTIVVVCIIFLLLSNYRILKRLCHVLHGVIFCRPRAHFEENPNDASPQIHGDGLDASM 80
           S+ T +V+ +  LL +    L  +  VL  V+ C  R    E+P D +  +   G+    
Sbjct: 36  SMDTHMVIILAALLCALICAL-GINSVLRCVLRCTRRFTPNEDPVDTNANV-AKGIKKRA 95

Query: 81  IRLLPISQFKKNVEAGSPASINTECAVCLGDFEEGELIKHLPNCNHSFHSSCIDSWFRNH 140
           ++++P+  +   ++  +     TEC +CLGDF EGE ++ LP CNH FH  CID+W  +H
Sbjct: 96  LKVIPVDSYSPELKMKA-----TECLICLGDFVEGETVRVLPKCNHGFHVKCIDTWLLSH 155

Query: 141 SSCPLCRSHVLD 153
           SSCP CR  +L+
Sbjct: 156 SSCPTCRQSLLE 160

The following BLAST results are available for this feature:

BLAST of MS020687 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031743630.1	1.1e-77	75.37	RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical pro...	[more]
KAG6570731.1	8.1e-76	73.30	RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_023512261.1	3.4e-74	71.84	RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo]	[more]
XP_008448367.1	7.6e-74	72.41	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H...	[more]
XP_034689191.1	9.3e-40	48.21	RING-H2 finger protein ATL52-like [Vitis riparia]	[more]

BLAST of MS020687 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93823	1.7e-20	37.22	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LF64	1.9e-19	35.67	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9LSW9	4.2e-19	28.84	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
Q7XLY8	9.5e-19	32.42	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SRQ8	4.7e-18	32.40	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of MS020687 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCA5	5.5e-78	75.37	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE...	[more]
A0A5A7V3S2	3.7e-74	72.41	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BIX6	3.7e-74	72.41	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 ...	[more]
A0A438G1M9	7.2e-38	51.22	E3 ubiquitin-protein ligase OS=Vitis vinifera OX=29760 GN=Os04g0590900 PE=4 SV=1	[more]
A0A2C9W1B6	2.1e-37	47.50	RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G0302...	[more]

BLAST of MS020687 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04360.1	1.2e-21	37.22	RING/U-box superfamily protein	[more]
AT5G17600.1	1.4e-20	35.67	RING/U-box superfamily protein	[more]
AT5G43420.1	3.0e-20	28.84	RING/U-box superfamily protein	[more]
AT3G03550.1	3.3e-19	32.40	RING/U-box superfamily protein	[more]
AT5G05280.1	8.2e-18	34.85	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 105..146 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 104..147 e-value: 6.2E-13 score: 48.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 105..147 score: 12.622055
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 76..155 e-value: 1.7E-21 score: 77.2
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 14..160
None	No IPR available	PANTHER	PTHR45676:SF107	RING/U-BOX SUPERFAMILY PROTEIN	coord: 14..160
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 104..147 e-value: 3.5103E-17 score: 70.0233
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 104..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020687.1	MS020687.1	mRNA