MS020684 (gene) Bitter gourd (TR) v1

Overview


Name	MS020684
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like
Location	scaffold375: 2201289 .. 2202239 (+)
RNA-Seq Expression	MS020684
Synteny	MS020684

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACATTGGATCTTTTTTGCAAATCCAGAGCTTCTACGGCTGTTTGTTCAAGCCTGGACCGCCGTCCGGTGGCCGCCCGTGGCCACTCCGGCAACCGGAGAAAAGGGCAGCTTCATTTCGAGAAGCACAGAAAGAGTACGAGCTGTTCCAGTGCGAATCGAAAGGATGATTTTAATTTTCGAAGAAAAAGCTGTGCGGATGTGGATGATCTGAAGAGCCCTGCTTCCGGTTCCTCTGCTCGATATCTGTTGGGGGATTCGCCGTTCATTGACTGGTTTCCGGCCGCCTCCGGCGAGGATTTTCCGGCGAATCCGCCGGAGGAGAGAACACAGAAATCTTCTTCTTTGACTCGATCGTTGACTGTGCATGAGTATCGTGGCCTCAAATCTCCTTCCTCTGTTCTTTCTCACGAATCCCCTGTTCTAAAATCACCTTCCTCGGCTCAATCTCGTCATCAGGTTATTCTATTTAAAAATTCTCTCTAAATTTTCGTCATATGAAAGTTTCTGGCTCGACCCATGTGAGAGAATTGCGTTAGAATTGAAATGGATTTTTTAAGTTTTTTGTTCTTGGTGGTCTTGTTTTAAAAGGTGGTGGTTCTGAAGGTCTCCCTGAACTGCAAAGGCTGCGAAAAGAAGGTGAAGAAGCACATCTCCAAGATGGAAGGTGAGCAAAACAAGAGAGAAAGATCATGTTTAGAATTATGAACTGATGTAGTCTGTTAGTAAAATAATAAAAAAAGTATTGATAATTTTGCAGGAGTGACATCATATAGTGTGGATTTCACGACAAAAAAAGTGACCATAATTGGGGAGGTGACCCCTTTTGGTGTACTGGAGAGCGTGTCAAAAGTGAAGCATGCTCAGCTCTGGCCATCTCCAACTTCATCAACATCTTCTTCATCTTCATCTTCAACACCTCAATCTTCTGCATCATCCTCAACATTC

mRNA sequence

ATGAAAACATTGGATCTTTTTTGCAAATCCAGAGCTTCTACGGCTGTTTGTTCAAGCCTGGACCGCCGTCCGGTGGCCGCCCGTGGCCACTCCGGCAACCGGAGAAAAGGGCAGCTTCATTTCGAGAAGCACAGAAAGAGTACGAGCTGTTCCAGTGCGAATCGAAAGGATGATTTTAATTTTCGAAGAAAAAGCTGTGCGGATGTGGATGATCTGAAGAGCCCTGCTTCCGGTTCCTCTGCTCGATATCTGTTGGGGGATTCGCCGTTCATTGACTGGTTTCCGGCCGCCTCCGGCGAGGATTTTCCGGCGAATCCGCCGGAGGAGAGAACACAGAAATCTTCTTCTTTGACTCGATCGTTGACTGTGCATGAGTATCGTGGCCTCAAATCTCCTTCCTCTGTTCTTTCTCACGAATCCCCTGTTCTAAAATCACCTTCCTCGGCTCAATCTCGTCATCAGGTGGTGGTTCTGAAGGTCTCCCTGAACTGCAAAGGCTGCGAAAAGAAGGTGAAGAAGCACATCTCCAAGATGGAAGGAGTGACATCATATAGTGTGGATTTCACGACAAAAAAAGTGACCATAATTGGGGAGGTGACCCCTTTTGGTGTACTGGAGAGCGTGTCAAAAGTGAAGCATGCTCAGCTCTGGCCATCTCCAACTTCATCAACATCTTCTTCATCTTCATCTTCAACACCTCAATCTTCTGCATCATCCTCAACATTC

Coding sequence (CDS)

ATGAAAACATTGGATCTTTTTTGCAAATCCAGAGCTTCTACGGCTGTTTGTTCAAGCCTGGACCGCCGTCCGGTGGCCGCCCGTGGCCACTCCGGCAACCGGAGAAAAGGGCAGCTTCATTTCGAGAAGCACAGAAAGAGTACGAGCTGTTCCAGTGCGAATCGAAAGGATGATTTTAATTTTCGAAGAAAAAGCTGTGCGGATGTGGATGATCTGAAGAGCCCTGCTTCCGGTTCCTCTGCTCGATATCTGTTGGGGGATTCGCCGTTCATTGACTGGTTTCCGGCCGCCTCCGGCGAGGATTTTCCGGCGAATCCGCCGGAGGAGAGAACACAGAAATCTTCTTCTTTGACTCGATCGTTGACTGTGCATGAGTATCGTGGCCTCAAATCTCCTTCCTCTGTTCTTTCTCACGAATCCCCTGTTCTAAAATCACCTTCCTCGGCTCAATCTCGTCATCAGGTGGTGGTTCTGAAGGTCTCCCTGAACTGCAAAGGCTGCGAAAAGAAGGTGAAGAAGCACATCTCCAAGATGGAAGGAGTGACATCATATAGTGTGGATTTCACGACAAAAAAAGTGACCATAATTGGGGAGGTGACCCCTTTTGGTGTACTGGAGAGCGTGTCAAAAGTGAAGCATGCTCAGCTCTGGCCATCTCCAACTTCATCAACATCTTCTTCATCTTCATCTTCAACACCTCAATCTTCTGCATCATCCTCAACATTC

Protein sequence

MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFNFRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASSSTF

Homology

[Close]

BLAST of MS020684 vs. NCBI nr
Match: XP_022148318.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 448.0 bits (1151), Expect = 5.3e-122
Identity = 238/242 (98.35%), Postives = 240/242 (99.17%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MKTLDLFCKSRASTAVCSSLDRRPVAARG+SGNRRKGQLHFEKHRKSTSCSSANRKDDFN
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120
           FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120

Query: 121 LTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180
           LT HEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG
Sbjct: 121 LTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180

Query: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASSS 240
           VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTP+SSASS 
Sbjct: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASSP 240

Query: 241 TF 243
           TF
Sbjct: 241 TF 242

[Close]

BLAST of MS020684 vs. NCBI nr
Match: XP_022148319.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 433.7 bits (1114), Expect = 1.0e-117
Identity = 233/242 (96.28%), Postives = 235/242 (97.11%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MKTLDLFCKSRASTAVCSSLDRRPVAARG+SGNRRKGQLHFEKHRKSTSCSSANRKDDFN
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120
           FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120

Query: 121 LTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180
           LT HEYRGLKSPSSVLSHESPVLKSPSSAQSRHQ     VSLNCKGCEKKVKKHISKMEG
Sbjct: 121 LTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQ-----VSLNCKGCEKKVKKHISKMEG 180

Query: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASSS 240
           VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTP+SSASS 
Sbjct: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASSP 237

Query: 241 TF 243
           TF
Sbjct: 241 TF 237

[Close]

BLAST of MS020684 vs. NCBI nr
Match: XP_038901867.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like [Benincasa hispida])

HSP 1 Score: 335.9 bits (860), Expect = 2.9e-88
Identity = 192/248 (77.42%), Postives = 209/248 (84.27%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LDLFCKSRASTAV SS  RRPVA   HSG+RRKGQLHFE HRKSTSCS+ NRK+  +
Sbjct: 1   MKKLDLFCKSRASTAVRSSFGRRPVAGDAHSGDRRKGQLHFENHRKSTSCSTVNRKELND 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEER------TQKS 120
            RRKSCADVDDLKSP SGSSARYLLGDSPF+DWFPA SGEDFPA  PE+R      ++KS
Sbjct: 61  LRRKSCADVDDLKSPVSGSSARYLLGDSPFLDWFPAVSGEDFPAPMPEKRKIISNSSEKS 120

Query: 121 SSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKH 180
            SL+RSLTVHEYRGLKSPSSVL  ESPVLK+ S   SR QVVVLKVSLNCKGCEKKVKKH
Sbjct: 121 CSLSRSLTVHEYRGLKSPSSVL--ESPVLKTSSLTHSRDQVVVLKVSLNCKGCEKKVKKH 180

Query: 181 ISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQ 240
           ISKMEGVTSYSVDFTTKKVTIIG++TPF VL SVSKVK+AQ W SP SS+SS+  SS+  
Sbjct: 181 ISKMEGVTSYSVDFTTKKVTIIGDLTPFDVLASVSKVKYAQFWQSPNSSSSSTPQSSS-- 240

Query: 241 SSASSSTF 243
           SS+SSSTF
Sbjct: 241 SSSSSSTF 244

[Close]

BLAST of MS020684 vs. NCBI nr
Match: XP_004148220.1 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Cucumis sativus] >KGN47352.1 hypothetical protein Csa_023044 [Cucumis sativus])

HSP 1 Score: 331.6 bits (849), Expect = 5.5e-87
Identity = 189/248 (76.21%), Postives = 205/248 (82.66%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LD+FCKSRASTAV SS  RRP+    HSG+RRKGQLHFE HRKSTSCS+ NRK+  +
Sbjct: 1   MKKLDIFCKSRASTAVRSSFARRPLTGDAHSGDRRKGQLHFENHRKSTSCSTLNRKELND 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEER------TQKS 120
            RRKSCADVDDLKSP SGSSARYLLGDSPF+DWFPA SGE+ PA  PE+R      +QKS
Sbjct: 61  LRRKSCADVDDLKSPVSGSSARYLLGDSPFLDWFPAVSGEEVPALMPEKRKIISDNSQKS 120

Query: 121 SSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKH 180
             L RSLTV EY GLKSPSSVL  ESPVLK+PS  QSR QVVVLKVSLNC+GCEKKVKKH
Sbjct: 121 FLLNRSLTVREYGGLKSPSSVL--ESPVLKTPSLTQSRDQVVVLKVSLNCRGCEKKVKKH 180

Query: 181 ISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQ 240
           ISKMEGVTSYSVDFTTKKVTIIG++TPF VL SVSKVK AQ WPSP     +SSSSSTPQ
Sbjct: 181 ISKMEGVTSYSVDFTTKKVTIIGDITPFDVLASVSKVKSAQFWPSP-----NSSSSSTPQ 240

Query: 241 SSASSSTF 243
           SS+SSSTF
Sbjct: 241 SSSSSSTF 241

[Close]

BLAST of MS020684 vs. NCBI nr
Match: KAG6570732.1 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315.5 bits (807), Expect = 4.1e-82
Identity = 185/243 (76.13%), Postives = 198/243 (81.48%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LD FCKSRASTAV SS  RRPV    +SG+RRK QL FE HRKSTSCSS NRKD ++
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKS-SSLTR 120
            RRKSCADVDDLKS  SGSSARYLL DS FIDW PA SGEDFPA PP+ER   S SSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPKERNMISHSSLTR 120

Query: 121 SLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKME 180
           SLTVHEYRG KSPSSVL  +SPVLK+ SSA    QVVVLKVSLNCKGCEKKVKKHISKME
Sbjct: 121 SLTVHEYRGQKSPSSVL--QSPVLKTASSA-VHDQVVVLKVSLNCKGCEKKVKKHISKME 180

Query: 181 GVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASS 240
           GV+SYSVDFT KKVTIIG V+PF VL SVSKVK+AQ W SP+SS+ SSSSSS+   S SS
Sbjct: 181 GVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSS 240

Query: 241 STF 243
           STF
Sbjct: 241 STF 240

[Close]

BLAST of MS020684 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.8e-22
Identity = 66/138 (47.83%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 90  FIDWFPAASGEDFPANPPEERTQKSSSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSA 149
           F+D+ P  S    P NP  E T+  +S T SL+  E + + SP    S   P   SP  +
Sbjct: 183 FLDYEPVLS----PDNPFSEPTK--ASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQS 242

Query: 150 ---------QSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVT 209
                     S  QVVVL+VSL+CKGC  KVKKH+SK++GVTSY++DF  KKVT+ G+VT
Sbjct: 243 SPPSPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVT 302

Query: 210 PFGVLESVSKVKHAQLWP 219
           P  VL S+SKVK+AQ WP
Sbjct: 303 PLTVLASISKVKNAQFWP 314

[Close]

BLAST of MS020684 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-19
Identity = 43/78 (55.13%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 144 KSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVTPFG 203
           K  +   +  QVVVLKVSL+C+GCE KV+KH+++M+GVTS+++DF  KKVT+ G++TP  
Sbjct: 171 KKTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLE 230

Query: 204 VLESVSKVKHAQLWPSPT 222
           +L+S+SKVK+AQ W +PT
Sbjct: 231 ILDSISKVKNAQFWTNPT 248

[Close]

BLAST of MS020684 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.4e-16
Identity = 82/225 (36.44%), Postives = 109/225 (48.44%), Query Frame = 0


Query: 11  RASTAVCSSLDRRPVAAR---------GHSGNRRKGQLHFEKHRKSTSCSSANR-KDDFN 70
           R+ TA CSS D      R           SG+ +  Q+  +K R+S S S       D +
Sbjct: 40  RSFTAPCSSGDDYVAPYRQLSKITRVPSSSGDGKSVQV--DKGRRSNSGSLMKLISYDVS 99

Query: 71  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 130
             RKS   V        GS+ RYLLG  P       ++G+D  A    E +         
Sbjct: 100 LARKSFGCVVATPKTPPGST-RYLLGSDPVS--LAGSTGQDTVATEESEASAPK------ 159

Query: 131 LTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSL--NCKGCEKKVKKHISKM 190
                 RG   P           K  SS     QVVVL+VSL  +C+GC+ KVKKH+SKM
Sbjct: 160 ------RGSSGPVEE--------KKKSSGSGSDQVVVLRVSLHCHCRGCQGKVKKHLSKM 219

Query: 191 EGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSS 224
           +GVTS+++DF +KKVT+ G++TP  VL  +SKVK+AQ W  P  S
Sbjct: 220 QGVTSFNIDFASKKVTVTGDITPLEVLGCLSKVKNAQFWTPPPPS 239

[Close]

BLAST of MS020684 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 29/69 (42.03%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 152 RHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKV 211
           R+   VL+VS++C+GC++K+KK +SK++GV + ++D   +KVT+IG V P  +++ + K 
Sbjct: 28  RYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKA 87

Query: 212 -KHAQLWPS 220
            +HA+LWP+
Sbjct: 88  GRHAELWPT 96

[Close]

BLAST of MS020684 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 31/82 (37.80%), Postives = 52/82 (63.41%), Query Frame = 0


Query: 154 QVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKV-K 213
           Q  VLKV+++C GC++KVKK + K+EGV +  +D    KVT+ G V P  +++ ++K  K
Sbjct: 10  QTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGK 69

Query: 214 HAQLWPSPTSSTSSSSSSSTPQ 235
           HA++W +P  + + + S    Q
Sbjct: 70  HAEIWGAPKGNNNPNQSQMANQ 91

[Close]

BLAST of MS020684 vs. ExPASy TrEMBL
Match: A0A6J1D3S6 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017000 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.6e-122
Identity = 238/242 (98.35%), Postives = 240/242 (99.17%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MKTLDLFCKSRASTAVCSSLDRRPVAARG+SGNRRKGQLHFEKHRKSTSCSSANRKDDFN
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120
           FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120

Query: 121 LTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180
           LT HEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG
Sbjct: 121 LTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180

Query: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASSS 240
           VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTP+SSASS 
Sbjct: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASSP 240

Query: 241 TF 243
           TF
Sbjct: 241 TF 242

[Close]

BLAST of MS020684 vs. ExPASy TrEMBL
Match: A0A6J1D3N2 (protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017000 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.0e-118
Identity = 233/242 (96.28%), Postives = 235/242 (97.11%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MKTLDLFCKSRASTAVCSSLDRRPVAARG+SGNRRKGQLHFEKHRKSTSCSSANRKDDFN
Sbjct: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGYSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120
           FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS
Sbjct: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRS 120

Query: 121 LTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEG 180
           LT HEYRGLKSPSSVLSHESPVLKSPSSAQSRHQ     VSLNCKGCEKKVKKHISKMEG
Sbjct: 121 LTAHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQ-----VSLNCKGCEKKVKKHISKMEG 180

Query: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASSS 240
           VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTP+SSASS 
Sbjct: 181 VTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPKSSASSP 237

Query: 241 TF 243
           TF
Sbjct: 241 TF 237

[Close]

BLAST of MS020684 vs. ExPASy TrEMBL
Match: A0A0A0KG24 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G302690 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.7e-87
Identity = 189/248 (76.21%), Postives = 205/248 (82.66%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LD+FCKSRASTAV SS  RRP+    HSG+RRKGQLHFE HRKSTSCS+ NRK+  +
Sbjct: 1   MKKLDIFCKSRASTAVRSSFARRPLTGDAHSGDRRKGQLHFENHRKSTSCSTLNRKELND 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEER------TQKS 120
            RRKSCADVDDLKSP SGSSARYLLGDSPF+DWFPA SGE+ PA  PE+R      +QKS
Sbjct: 61  LRRKSCADVDDLKSPVSGSSARYLLGDSPFLDWFPAVSGEEVPALMPEKRKIISDNSQKS 120

Query: 121 SSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKH 180
             L RSLTV EY GLKSPSSVL  ESPVLK+PS  QSR QVVVLKVSLNC+GCEKKVKKH
Sbjct: 121 FLLNRSLTVREYGGLKSPSSVL--ESPVLKTPSLTQSRDQVVVLKVSLNCRGCEKKVKKH 180

Query: 181 ISKMEGVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQ 240
           ISKMEGVTSYSVDFTTKKVTIIG++TPF VL SVSKVK AQ WPSP     +SSSSSTPQ
Sbjct: 181 ISKMEGVTSYSVDFTTKKVTIIGDITPFDVLASVSKVKSAQFWPSP-----NSSSSSTPQ 240

Query: 241 SSASSSTF 243
           SS+SSSTF
Sbjct: 241 SSSSSSTF 241

[Close]

BLAST of MS020684 vs. ExPASy TrEMBL
Match: A0A6J1J8X9 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484523 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.6e-82
Identity = 185/243 (76.13%), Postives = 197/243 (81.07%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LD FCKSRASTAV SS  RRPV    +SG+RRK QL FE HRKSTSCSS NRKD ++
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDANSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKS-SSLTR 120
            RRKSCADVDDLKS  SGSSARYLL DS FIDW PA SGEDFPA PP ER   S SSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKME 180
           SLTVHEYRG KSPSSVL  +SPVLK+ SSA    QVVVLKVSLNCKGCEKKVKKHISKME
Sbjct: 121 SLTVHEYRGQKSPSSVL--QSPVLKTASSA-VHDQVVVLKVSLNCKGCEKKVKKHISKME 180

Query: 181 GVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSSTSSSSSSSTPQSSASS 240
           GV+SYSVDFT KKVTIIG V+PF VL SVSKVK+AQ W SP+SS+ SSSSSS+   S SS
Sbjct: 181 GVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSPSSSSCSSSSSSSTPQSCSS 240

Query: 241 STF 243
           STF
Sbjct: 241 STF 240

[Close]

BLAST of MS020684 vs. ExPASy TrEMBL
Match: A0A6J1FTK0 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448670 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.9e-81
Identity = 188/245 (76.73%), Postives = 199/245 (81.22%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCSSANRKDDFN 60
           MK LD FCKSRASTAV SS  RRPV    +SG+RRK QL FE HRKSTSCSS NRKD ++
Sbjct: 1   MKKLDFFCKSRASTAVRSSFGRRPVDGDENSGDRRKWQLPFENHRKSTSCSSVNRKDHYD 60

Query: 61  FRRKSCADVDDLKSPASGSSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKS-SSLTR 120
            RRKSCADVDDLKS  SGSSARYLL DS FIDW PA SGEDFPA PP ER   S SSLTR
Sbjct: 61  LRRKSCADVDDLKSSVSGSSARYLLSDSSFIDWLPAVSGEDFPAKPPNERNMISHSSLTR 120

Query: 121 SLTVHEYRGLKSPSSVLSHESPVLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKME 180
           SLTVHEYRG KSPSSVL  +SPVLK+ SSA    QVVVLKVSLNCKGCEKKVKKHISKME
Sbjct: 121 SLTVHEYRGQKSPSSVL--QSPVLKTASSA-VHDQVVVLKVSLNCKGCEKKVKKHISKME 180

Query: 181 GVTSYSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPTSS--TSSSSSSSTPQSSA 240
           GV+SYSVDFT KKVTIIG V+PF VL SVSKVK+AQ W S +SS  +SSSSSSSTPQ S 
Sbjct: 181 GVSSYSVDFTAKKVTIIGAVSPFDVLASVSKVKYAQFWTSLSSSSCSSSSSSSSTPQ-SC 240

Query: 241 SSSTF 243
           SSSTF
Sbjct: 241 SSSTF 241

[Close]

BLAST of MS020684 vs. TAIR 10
Match: AT2G28660.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 125.9 bits (315), Expect = 4.3e-29
Identity = 103/265 (38.87%), Postives = 131/265 (49.43%), Query Frame = 0


Query: 1   MKTLDLFCKSRASTAVCSSLDRRPVAARGHSGNRRKGQLHFEKHRKSTSCS--------- 60
           M+  D+FC S ASTAV  S          H GN   G+  F+ H +S + S         
Sbjct: 1   MRFSDIFCSSPASTAVRHS-------TLHHDGNVTGGRRSFQSHLRSQNPSKKKDKTVPC 60

Query: 61  ------------------------------SANRKDDFNFRRKSCADVDDLKSPAS---G 120
                                         ++ R  D   RRKS ADV DL+   S    
Sbjct: 61  FSSEMPLIPIPRHLSCRNSFESPSGFRPKIASARGSDVQIRRKSSADVSDLRRSRSSLLS 120

Query: 121 SSARYLLGDSPFIDWFPAASGEDFPANPPEERTQKSSSLTRSLTVHEYRGLKSPSSVLSH 180
           SS+RYLL D        +  G+D       +R+ K   L R   V       S SS  S 
Sbjct: 121 SSSRYLLKD------HKSLKGDDKDLWLSSDRS-KDLILYRDRNVTSSASSSSSSSSSSF 180

Query: 181 ESPVLKSPSSAQSR-HQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIG 223
            S V    S A S   QVVVL+VS++CKGCE KV+KHISKMEGVTSY++D  TKKVT++G
Sbjct: 181 SSSVTNVSSPAPSTDDQVVVLRVSIHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVG 240

[Close]

BLAST of MS020684 vs. TAIR 10
Match: AT5G02600.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 2.7e-23
Identity = 66/138 (47.83%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 90  FIDWFPAASGEDFPANPPEERTQKSSSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSA 149
           F+D+ P  S    P NP  E T+  +S T SL+  E + + SP    S   P   SP  +
Sbjct: 183 FLDYEPVLS----PDNPFSEPTK--ASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQS 242

Query: 150 ---------QSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVT 209
                     S  QVVVL+VSL+CKGC  KVKKH+SK++GVTSY++DF  KKVT+ G+VT
Sbjct: 243 SPPSPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVT 302

Query: 210 PFGVLESVSKVKHAQLWP 219
           P  VL S+SKVK+AQ WP
Sbjct: 303 PLTVLASISKVKNAQFWP 314

[Close]

BLAST of MS020684 vs. TAIR 10
Match: AT5G02600.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 2.7e-23
Identity = 66/138 (47.83%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 90  FIDWFPAASGEDFPANPPEERTQKSSSLTRSLTVHEYRGLKSPSSVLSHESPVLKSPSSA 149
           F+D+ P  S    P NP  E T+  +S T SL+  E + + SP    S   P   SP  +
Sbjct: 183 FLDYEPVLS----PDNPFSEPTK--ASPTASLSSLEDKDVSSPDFKFSPPPPPPPSPPQS 242

Query: 150 ---------QSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVT 209
                     S  QVVVL+VSL+CKGC  KVKKH+SK++GVTSY++DF  KKVT+ G+VT
Sbjct: 243 SPPSPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVT 302

Query: 210 PFGVLESVSKVKHAQLWP 219
           P  VL S+SKVK+AQ WP
Sbjct: 303 PLTVLASISKVKNAQFWP 314

[Close]

BLAST of MS020684 vs. TAIR 10
Match: AT2G37390.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.2e-20
Identity = 43/78 (55.13%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 144 KSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTSYSVDFTTKKVTIIGEVTPFG 203
           K  +   +  QVVVLKVSL+C+GCE KV+KH+++M+GVTS+++DF  KKVT+ G++TP  
Sbjct: 171 KKTTCGDTDQQVVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLE 230

Query: 204 VLESVSKVKHAQLWPSPT 222
           +L+S+SKVK+AQ W +PT
Sbjct: 231 ILDSISKVKNAQFWTNPT 248

[Close]

BLAST of MS020684 vs. TAIR 10
Match: AT2G37390.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 95.5 bits (236), Expect = 6.2e-20
Identity = 45/98 (45.92%), Postives = 71/98 (72.45%), Query Frame = 0


Query: 130 KSPSSVLSHESP------VLKSPSSAQSRHQVVVLKVSLNCKGCEKKVKKHISKMEGVTS 189
           + P+  +  E+P      + +  ++     QVVVLKVSL+C+GCE KV+KH+++M+GVTS
Sbjct: 150 QDPAKTVEAEAPAGEDKTLTEKKTTCGDTDQVVVLKVSLHCRGCEGKVRKHLARMQGVTS 209

Query: 190 YSVDFTTKKVTIIGEVTPFGVLESVSKVKHAQLWPSPT 222
           +++DF  KKVT+ G++TP  +L+S+SKVK+AQ W +PT
Sbjct: 210 FNIDFAAKKVTVTGDITPLEILDSISKVKNAQFWTNPT 247

The following BLAST results are available for this feature:

BLAST of MS020684 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148318.1	5.3e-122	98.35	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 [Momordica charantia]	[more]
XP_022148319.1	1.0e-117	96.28	protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 [Momordica charantia]	[more]
XP_038901867.1	2.9e-88	77.42	protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like [Benincasa hispida]	[more]
XP_004148220.1	5.5e-87	76.21	protein SODIUM POTASSIUM ROOT DEFECTIVE 1 [Cucumis sativus] >KGN47352.1 hypothet...	[more]
KAG6570732.1	4.1e-82	76.13	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of MS020684 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDS4	3.8e-22	47.83	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q58FZ0	1.8e-19	55.13	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q8RXH8	1.4e-16	36.44	Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q84J88	1.6e-09	42.03	Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX...	[more]
Q9M8K5	2.9e-06	37.80	Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX...	[more]

BLAST of MS020684 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3S6	2.6e-122	98.35	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like isoform X1 OS=Momordica charantia...	[more]
A0A6J1D3N2	5.0e-118	96.28	protein SODIUM POTASSIUM ROOT DEFECTIVE 2-like isoform X2 OS=Momordica charantia...	[more]
A0A0A0KG24	2.7e-87	76.21	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G302690 PE=4 SV...	[more]
A0A6J1J8X9	2.6e-82	76.13	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FTK0	4.9e-81	76.73	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of MS020684 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28660.1	4.3e-29	38.87	Chloroplast-targeted copper chaperone protein	[more]
AT5G02600.2	2.7e-23	47.83	Heavy metal transport/detoxification superfamily protein	[more]
AT5G02600.1	2.7e-23	47.83	Heavy metal transport/detoxification superfamily protein	[more]
AT2G37390.1	1.2e-20	55.13	Chloroplast-targeted copper chaperone protein	[more]
AT2G37390.2	6.2e-20	45.92	Chloroplast-targeted copper chaperone protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 151..215 e-value: 4.7E-15 score: 57.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 216..242
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 26..54
None	No IPR available	PANTHER	PTHR46119:SF12	HEAVY METAL-ASSOCIATED DOMAIN CONTAINING PROTEIN, EXPRESSED	coord: 1..231
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 159..210 e-value: 8.2E-12 score: 45.4
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 154..211 score: 10.675345
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 157..214 e-value: 3.64124E-11 score: 55.3045
IPR044526	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3	PANTHER	PTHR46119	OS08G0405700 PROTEIN	coord: 1..231
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 153..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS020684.1	MS020684.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding