MS020536 (gene) Bitter gourd (TR) v1

BLAST of MS020536 vs. NCBI nr
Match: XP_022947943.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 445.7 bits (1145), Expect = 3.3e-121
Identity = 216/299 (72.24%), Postives = 248/299 (82.94%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           M NI H+Y+R LRW MK AGVR ELVQIEPGT MNFWVPTRNP  N NP    P+VLLHG
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNP----PVVLLHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FA N I++WQFQV+ LARNY VY PDF+FFG+SVTDR ERSPEFQAE VVKGLRKLGVER
Sbjct: 61  FAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVER 120

Query: 121 -CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
            CVLVGFSYG MVGFRLAE++P+ VAAMVVT +P ALTESLSR +L EIGYKSW DYLIP
Sbjct: 121 PCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIP 180

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TV  V+S+FEI S+  P LP+W  KHYLEAM+ +RKE+AELL AL+  DDFTI+++PQ+
Sbjct: 181 NTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQK 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSL 299
           M IIWG NDNLF++QIAY MKE+ GG+ ++DCIEKAGHIV MERP IYNK L+KFL SL
Sbjct: 241 MHIIWGRNDNLFNMQIAYNMKEQFGGETRLDCIEKAGHIVPMERPFIYNKYLKKFLRSL 295

BLAST of MS020536 vs. NCBI nr
Match: XP_038902558.1 (epoxide hydrolase 4-like [Benincasa hispida])

HSP 1 Score: 442.2 bits (1136), Expect = 3.6e-120
Identity = 208/301 (69.10%), Postives = 250/301 (83.06%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI H+Y+R +RW MKAAGVRP  VQI+PGT MNFWVP  NP+     +P  PL+LLHG
Sbjct: 1   MVNIFHIYDRVVRWVMKAAGVRPVQVQIKPGTTMNFWVPAGNPNKIHQNHP--PLLLLHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FAANAI++WQFQVL LA+ Y VY PDFIFFG+SVTDRP+RSPEFQAECV +GLRKLGVER
Sbjct: 61  FAANAIMTWQFQVLNLAKKYAVYVPDFIFFGESVTDRPDRSPEFQAECVAEGLRKLGVER 120

Query: 121 -CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
            CVLVGFSYG MVGFR+AE++PE V AMVVT +P  L ES+SR SLE+IG+KSW +YLIP
Sbjct: 121 GCVLVGFSYGAMVGFRMAEMYPELVEAMVVTAAPTVLMESISRESLEKIGFKSWSEYLIP 180

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            + + V+S+ EIAS+K P +P W FKHYLEAM++H+KERAELL  L++ADD  I++YPQ+
Sbjct: 181 ESAEGVKSLLEIASFKFPRVPRWIFKHYLEAMVMHKKERAELLRELVAADDVIISQYPQK 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSLL 300
           + +IWG NDNLFD+QIAY M E+ GGK ++DCIEKAGHIV +ERP IYNKCLQ FLHSLL
Sbjct: 241 LHVIWGRNDNLFDVQIAYNMIEEFGGKARLDCIEKAGHIVPLERPFIYNKCLQNFLHSLL 299

BLAST of MS020536 vs. NCBI nr
Match: XP_004148865.1 (epoxide hydrolase 3 [Cucumis sativus] >KGN61797.1 hypothetical protein Csa_006154 [Cucumis sativus])

HSP 1 Score: 430.6 bits (1106), Expect = 1.1e-116
Identity = 203/299 (67.89%), Postives = 245/299 (81.94%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI H+Y++ LRW MKAAG++P  +QI+PGTAMNFWVP   P+ N   +P  PL+ LHG
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHP--PLLFLHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVE- 120
           FA NAI++WQFQVL  A+N+ VY PDF+FFGDSVTDRP+R+ EFQAECVV+GLRKLGV+ 
Sbjct: 61  FATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDR 120

Query: 121 RCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
           R VLVGFSYG MVGFRLAE++PE V AMVVT +P  LTE ++  ++E+IGYKSW +YLIP
Sbjct: 121 RFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIP 180

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TVK   SM +IAS++ P  P W FK YLEAM++HRKERAELLEAL++ +D TI++YPQ+
Sbjct: 181 ETVKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQK 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSL 299
           + IIWG NDNLFD+QIAY MKEK G K  +DCIEKAGHIVAMERP IYNKCLQKFLHSL
Sbjct: 241 LHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSL 297

BLAST of MS020536 vs. NCBI nr
Match: XP_016902619.1 (PREDICTED: uncharacterized protein LOC103499739 [Cucumis melo])

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-100
Identity = 178/262 (67.94%), Postives = 212/262 (80.92%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           ++NI H+Y+  LRW MKAAG+ P  +QIEPGT+MNFWVP R P+ N   +P  PL+ LHG
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHP--PLLFLHG 61

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVE- 120
           FAANAI++WQFQVL  A+ + VY PDF+FFGDSVTDRP+RSPEFQAECV +GLRKLGVE 
Sbjct: 62  FAANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVER 121

Query: 121 RCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
           R VLVGFSYG MVGFRLAE +PE V AMVVT +P  LTE ++  +LE+IGYKSW +YLIP
Sbjct: 122 RFVLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIP 181

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TVK V+SM EIAS++ P  P W FKHYLE MIIH KERAELLEAL++ +D TI+RYPQ+
Sbjct: 182 ETVKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQK 241

Query: 241 MLIIWGENDNLFDLQIAYKMKE 262
           + IIWG NDNLFD+QIAY MKE
Sbjct: 242 LHIIWGRNDNLFDIQIAYNMKE 261

BLAST of MS020536 vs. NCBI nr
Match: KAF3967813.1 (hypothetical protein CMV_008228 [Castanea mollissima])

HSP 1 Score: 374.8 bits (961), Expect = 7.2e-100
Identity = 174/307 (56.68%), Postives = 234/307 (76.22%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI  +Y   LRW MK  G+RP++V+IEPGT MNFWVP   P  + N N N  +V +HG
Sbjct: 1   MVNIFAVYKPLLRWVMKLVGMRPQIVEIEPGTIMNFWVPHETPKKSKNSNNNKAVVFIHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FAA+ I++WQ QVLALAR Y VY PD +FFG S+TDR ERSPEFQAECV KGL+KLGVER
Sbjct: 61  FAADGIITWQSQVLALARKYKVYVPDLVFFGGSITDRSERSPEFQAECVAKGLKKLGVER 120

Query: 121 CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIPA 180
           C LVG SYGGM+GF++AE++P+ V +MV+T S  ALT+S+S  +LE IG+KSW DYL+P 
Sbjct: 121 CTLVGLSYGGMIGFKMAEMYPDLVESMVLTCSVMALTKSISDAALERIGFKSWSDYLLPD 180

Query: 181 TVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISAD-DFTITRYPQR 240
           +V+ V+ +F+IA++KLP +P + +KHYLE M  +RKER ELL AL+  D DFTI  +PQR
Sbjct: 181 SVQGVKVLFDIATFKLPRIPSFIYKHYLEVMFDNRKEREELLLALVIDDNDFTIPSHPQR 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSLL 300
           + ++WGEND +F++++   +K ++G +  +  IEKAGH+V +ERPC+YNK L++ L +LL
Sbjct: 241 VHLLWGENDIIFNMEVVNNLKRQLGDRATLQYIEKAGHLVEVERPCVYNKHLKEILTTLL 300

Query: 301 EE-DSTQ 306
           E+ D TQ
Sbjct: 301 EDGDQTQ 307

BLAST of MS020536 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 8.1e-14
Identity = 69/267 (25.84%), Postives = 115/267 (43.07%), Query Frame = 0

Query: 55  LVLLHGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLR 114
           ++LLHGF      SW++Q+      Y V A D   +G+  TD P     ++ +C++  ++
Sbjct: 96  MLLLHGF-PEFWYSWRYQLREFKSEYRVVALDLRGYGE--TDAPIHRQNYKLDCLITDIK 155

Query: 115 ----KLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTG--SPAALTESLSRGSLEEI 174
                LG  +CVL+G  +GGM+ + +A  +PE V  ++V     P   TE + R   + +
Sbjct: 156 DILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIVINFPHPNVFTEYILRHPAQLL 215

Query: 175 GYKSWPDYLIPATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALI-- 234
               +  + IP   +    MF I  +K+  L   F  H         +   E LEA I  
Sbjct: 216 KSSYYYFFQIPWFPE---FMFSINDFKV--LKHLFTSHSTGIGRKGCQLTTEDLEAYIYV 275

Query: 235 ----SADDFTITRY--------------PQRMLIIWGENDNLFDLQIAYKMKEKVGGKVK 294
                A    I  Y                  L++WGEND   ++++A   K  V    +
Sbjct: 276 FSQPGALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGENDAFMEVEMAEVTKIYVKNYFR 335

Query: 295 VDCIEKAGHIVAMERPCIYNKCLQKFL 296
           +  + +A H +  ++P I NK +  FL
Sbjct: 336 LTILSEASHWLQQDQPDIVNKLIWTFL 354

BLAST of MS020536 vs. ExPASy Swiss-Prot
Match: Q0IIS3 (Epoxide hydrolase 3 OS=Xenopus tropicalis OX=8364 GN=ephx3 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.4e-13
Identity = 67/269 (24.91%), Postives = 115/269 (42.75%), Query Frame = 0

Query: 55  LVLLHGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGL- 114
           ++LLHGF  N   SW++Q+   +  Y   A D   FG S  D P R  +++ E +++ L 
Sbjct: 100 MLLLHGFPEN-WYSWRYQLDEFSNGYRTVAIDLRGFGGS--DAPSRLEDYKMEILLQDLQ 159

Query: 115 ---RKLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSP-------AALTESLSRG 174
              R LG  RCVLVG  +GG + +  A    + V  ++V  +P         L+      
Sbjct: 160 DLIRGLGYSRCVLVGHDWGGTLAWTFAVRHRDMVTHLIVMNAPHPSAFHDYVLSHPSQLF 219

Query: 175 SLEEIGYKSWPDYLIPATVKDVRSMFEI------ASYKLPPLPDWFFKHYLEAMIIHRKE 234
           S   +     P  LIP  +  +R    I      A++ +  +     K  +EA + +  +
Sbjct: 220 SSRYVFLFQLP--LIPEILLSLRDFEHIKKPLTDATHGIQNVECKLSKEEVEAFVYYPSQ 279

Query: 235 RAELLEAL-----------ISADDFTITRYPQRMLIIWGENDNLFDLQIAYKMKEKVGGK 294
           +  L   L           + A D  +       L++WGE+D   +  +  +M++ V   
Sbjct: 280 KGALTPPLNYYRNLFGFFPVKAQDVLVP-----TLLLWGEHDAFLEAAMVPEMQQYVRAP 339

Query: 295 VKVDCIEKAGHIVAMERPCIYNKCLQKFL 296
            + + I  A H +  +RP   NK ++ FL
Sbjct: 340 FRAEIIPNASHWLQQDRPQEVNKIIRDFL 358

BLAST of MS020536 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 2.6e-12
Identity = 68/279 (24.37%), Postives = 115/279 (41.22%), Query Frame = 0

Query: 55  LVLLHGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLR 114
           ++LLHGF      SW+ Q+      Y V A D   +G+S  D P     ++ +C++  ++
Sbjct: 94  MLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGES--DAPAHQESYKLDCLIADIK 153

Query: 115 ----KLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTG--SPAALTESLSRGSLEEI 174
                LG  +CVL+G  +GGM+ + +A  +PE +  ++V     P+  TE + R   +  
Sbjct: 154 DILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPAQLF 213

Query: 175 GYKSWPDYLIPATVKDVRSMFEIASYKLPPLPDWFFKHYL--EAMIIHRKER---AELLE 234
               +  + IP   +    MF I  +K         KH    ++  I RK R    E LE
Sbjct: 214 RSSFYYFFQIP---RFPEFMFSINDFKA-------LKHLFTSQSTGIGRKGRQLTTEDLE 273

Query: 235 ALI------SADDFTITRY--------------PQRMLIIWGENDNLFDLQIAYKMKEKV 294
           A +       A    I  Y                  L++WGE D   ++++A   K  V
Sbjct: 274 AYVYVFSQPGALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGEEDAFMEVEMAEVTKIYV 333

Query: 295 GGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSLLEED 303
               ++  + +  H +  ++P I N  +  FL      D
Sbjct: 334 KNYFRLTILSEGSHWLQQDQPDIVNGLIWAFLKEETRRD 359

BLAST of MS020536 vs. ExPASy Swiss-Prot
Match: G5EBI4 (Epoxide hydrolase 1 OS=Caenorhabditis elegans OX=6239 GN=ceeh-1 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.1e-06
Identity = 65/287 (22.65%), Postives = 116/287 (40.42%), Query Frame = 0

Query: 54  PLVL-LHGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKG 113
           PL+L +HG+      SW+FQ+   A  Y   A D    G +++D+P+    +  + +   
Sbjct: 140 PLMLFIHGY-PEFWYSWRFQLKEFADKYRCVAID--QRGYNLSDKPKHVDNYSIDELTGD 199

Query: 114 LRK----LGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEI 173
           +R     LG ++ ++V   +GG+V ++ AE +PE V  ++    P         GS  + 
Sbjct: 200 IRDVIEGLGYDKAIVVAHDWGGLVAWQFAEQYPEMVDKLICCNIPRP-------GSFRKR 259

Query: 174 GYKSWPDY------------LIPATV--KDVRSMFEIA------------SYKLPPLPDW 233
            Y SW  +             IP  +   D   M E+             ++    L  W
Sbjct: 260 IYTSWSQFRKSWYMFFYQNEKIPEMLCSADDMKMLELCFRAKEIGIQNNKNFTDEDLEAW 319

Query: 234 FFK------------HYLEAMIIHRKERAELLEALISADDFTITRYPQRMLIIWGENDNL 293
            +             +Y   +   +K++A+L+  + +             LIIWG  D  
Sbjct: 320 KYSFSMNGASFKYPINYYRNIFNAKKQQADLVLEMPT-------------LIIWGTADGA 379

Query: 294 FDLQIAY-KMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLH 297
            D++ A   +     G +K   IE A H V  + P + N+ ++KFL+
Sbjct: 380 LDIEAAVDSLNTLKQGTMKK--IEGASHWVQQDEPEMVNEHIKKFLN 401

BLAST of MS020536 vs. ExPASy Swiss-Prot
Match: Q52532 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseudomonas sp. OX=306 GN=mhpC PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 5.3e-05
Identity = 41/186 (22.04%), Postives = 76/186 (40.86%), Query Frame = 0

Query: 115 KLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWP 174
           KLG++R  ++G S GG    R+A  +PE V  +V+ G  +    + +    E  G K   
Sbjct: 99  KLGIKRAHMIGNSMGGATAMRMAVDYPEMVGKLVMMGGGSVGGSTTTPMPTE--GLKLLQ 158

Query: 175 DYLIPATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADD---- 234
                 +++++R M +I  Y    L +       E M+   +     +E+L ++      
Sbjct: 159 GLYRNPSMENLRKMLDIFVYAPSTLTEELINGRFENMMRRPEHLTNFVESLKASGGRANY 218

Query: 235 -FTITRYPQRMLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNK 294
              +       +IIWG +D    L +  +M   +    ++    K GH    E    +N+
Sbjct: 219 AHLLPTLTMPTMIIWGRDDRFVPLDLGLRMLWGM-PDAELHVFSKCGHWAQWEHADKFNQ 278

Query: 295 CLQKFL 296
            +  FL
Sbjct: 279 LVLNFL 281

BLAST of MS020536 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.6e-121
Identity = 216/299 (72.24%), Postives = 248/299 (82.94%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           M NI H+Y+R LRW MK AGVR ELVQIEPGT MNFWVPTRNP  N NP    P+VLLHG
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNP----PVVLLHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FA N I++WQFQV+ LARNY VY PDF+FFG+SVTDR ERSPEFQAE VVKGLRKLGVER
Sbjct: 61  FAGNGIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVER 120

Query: 121 -CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
            CVLVGFSYG MVGFRLAE++P+ VAAMVVT +P ALTESLSR +L EIGYKSW DYLIP
Sbjct: 121 PCVLVGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIP 180

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TV  V+S+FEI S+  P LP+W  KHYLEAM+ +RKE+AELL AL+  DDFTI+++PQ+
Sbjct: 181 NTVNGVKSLFEIGSFDFPALPNWIMKHYLEAMVKYRKEQAELLRALVVPDDFTISQFPQK 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSL 299
           M IIWG NDNLF++QIAY MKE+ GG+ ++DCIEKAGHIV MERP IYNK L+KFL SL
Sbjct: 241 MHIIWGRNDNLFNMQIAYNMKEQFGGETRLDCIEKAGHIVPMERPFIYNKYLKKFLRSL 295

BLAST of MS020536 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.3e-117
Identity = 203/299 (67.89%), Postives = 245/299 (81.94%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI H+Y++ LRW MKAAG++P  +QI+PGTAMNFWVP   P+ N   +P  PL+ LHG
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHP--PLLFLHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVE- 120
           FA NAI++WQFQVL  A+N+ VY PDF+FFGDSVTDRP+R+ EFQAECVV+GLRKLGV+ 
Sbjct: 61  FATNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDR 120

Query: 121 RCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
           R VLVGFSYG MVGFRLAE++PE V AMVVT +P  LTE ++  ++E+IGYKSW +YLIP
Sbjct: 121 RFVLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIP 180

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TVK   SM +IAS++ P  P W FK YLEAM++HRKERAELLEAL++ +D TI++YPQ+
Sbjct: 181 ETVKGAISMLQIASFEFPRFPRWIFKQYLEAMVVHRKERAELLEALVAPNDVTISQYPQK 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSL 299
           + IIWG NDNLFD+QIAY MKEK G K  +DCIEKAGHIVAMERP IYNKCLQKFLHSL
Sbjct: 241 LHIIWGRNDNLFDIQIAYNMKEKFGEKATMDCIEKAGHIVAMERPFIYNKCLQKFLHSL 297

BLAST of MS020536 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.6e-100
Identity = 178/262 (67.94%), Postives = 212/262 (80.92%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           ++NI H+Y+  LRW MKAAG+ P  +QIEPGT+MNFWVP R P+ N   +P  PL+ LHG
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHP--PLLFLHG 61

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVE- 120
           FAANAI++WQFQVL  A+ + VY PDF+FFGDSVTDRP+RSPEFQAECV +GLRKLGVE 
Sbjct: 62  FAANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVER 121

Query: 121 RCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIP 180
           R VLVGFSYG MVGFRLAE +PE V AMVVT +P  LTE ++  +LE+IGYKSW +YLIP
Sbjct: 122 RFVLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIP 181

Query: 181 ATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISADDFTITRYPQR 240
            TVK V+SM EIAS++ P  P W FKHYLE MIIH KERAELLEAL++ +D TI+RYPQ+
Sbjct: 182 ETVKGVKSMLEIASFEFPRFPRWIFKHYLEGMIIHNKERAELLEALVTPNDVTISRYPQK 241

Query: 241 MLIIWGENDNLFDLQIAYKMKE 262
           + IIWG NDNLFD+QIAY MKE
Sbjct: 242 LHIIWGRNDNLFDIQIAYNMKE 261

BLAST of MS020536 vs. ExPASy TrEMBL
Match: A0A7N2R8U7 (AB hydrolase-1 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.8e-99
Identity = 174/307 (56.68%), Postives = 232/307 (75.57%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI  +Y   L W MK  GVRP+ V+IEPGT MNFWVP   P  + N N N  +V +HG
Sbjct: 1   MVNIFAVYKPLLHWVMKLVGVRPQRVEIEPGTIMNFWVPKETPKKSKNSNNNKAVVFIHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FA + I++WQ QVLALAR Y VY PD +FFG S+TDR ERSPEFQAECV KGL+KLGVER
Sbjct: 61  FADDGIITWQSQVLALARKYKVYVPDLVFFGGSITDRSERSPEFQAECVAKGLKKLGVER 120

Query: 121 CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIPA 180
           C LVG SYGGMVGF++AE++P+ V +MV+T S  ALT+S+S  +LE IG+KSW DYL+P 
Sbjct: 121 CTLVGLSYGGMVGFKMAEMYPDLVESMVLTCSVMALTKSISDAALERIGFKSWSDYLLPD 180

Query: 181 TVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISAD-DFTITRYPQR 240
           +V+ V+ +F+IA++KLP +P++ +KH+LE M  +RKER ELLEAL+  D DFTI  +PQR
Sbjct: 181 SVEGVKILFDIATFKLPRIPNFIYKHHLEIMFDNRKEREELLEALVIDDNDFTIPSHPQR 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSLL 300
           + ++WGEND +F++++   +K ++G +  +  IEKAGH+V  ERPC+YNK L++ L +LL
Sbjct: 241 VHLLWGENDIIFNMEVVNNLKRQLGDRATLQYIEKAGHLVEAERPCVYNKHLKEILTTLL 300

Query: 301 EE-DSTQ 306
           E+ D TQ
Sbjct: 301 EDGDQTQ 307

BLAST of MS020536 vs. ExPASy TrEMBL
Match: A0A2N9I7R2 (AB hydrolase-1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS48330 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.5e-98
Identity = 175/302 (57.95%), Postives = 231/302 (76.49%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHG 60
           MVNI  +Y   LR  MK AGVRP+ V+IEPGT MNFWVP +  + N        +V +HG
Sbjct: 1   MVNIFAIYKPVLRVLMKLAGVRPQTVEIEPGTVMNFWVPNQTSNKN-------TVVFIHG 60

Query: 61  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 120
           FAA+ I++WQFQVLALAR Y VY PD +FFG S+TDRPER+PEFQAECV KGLRKLGVE+
Sbjct: 61  FAADGIITWQFQVLALARKYKVYVPDLLFFGGSITDRPERTPEFQAECVAKGLRKLGVEK 120

Query: 121 CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIPA 180
           C LVGFSYGGMVGF++AE++P+ V +MV+T S  ALT+S+S   LE IG+KSW DYLIP 
Sbjct: 121 CTLVGFSYGGMVGFKMAEMYPDLVESMVLTCSVMALTKSISDAGLERIGFKSWSDYLIPD 180

Query: 181 TVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEALISAD-DFTITRYPQR 240
           +V+ V+ +FEIA+YKLP +P++ FK YLE M  +RKE++ELLEAL+  D DFTI ++PQR
Sbjct: 181 SVQGVKLLFEIATYKLPRIPNFVFKDYLEVMFDNRKEQSELLEALVIDDKDFTIAQHPQR 240

Query: 241 MLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFLHSLL 300
           + ++WGEND +F+ ++   +K ++G +  +  IEKAGH+V  ERPC+YNK L++ L +LL
Sbjct: 241 VYLLWGENDIIFNKEVVNNLKRQLGDRGTLQYIEKAGHLVEAERPCVYNKHLKEILATLL 295

Query: 301 EE 302
           E+
Sbjct: 301 ED 295

BLAST of MS020536 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.2e-83
Identity = 154/313 (49.20%), Postives = 214/313 (68.37%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVP------TRNPDPNPNPNPNIP 60
           MVN V L    L   MK AGV P  V++EPGT MNFW+P      T+  D N    P  P
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 61  ----LVLLHGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVV 120
               L+ +HGFAA  I++WQFQV +LA+ Y VY PD +FFG S +D  +RSP FQA C+V
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 121 KGLRKLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGY 180
           K LR LG+E+  LVGFSYGGMV F++AE +PE V AMVV+GS  A+T+++S  +L ++G+
Sbjct: 121 KSLRILGIEKFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLGF 180

Query: 181 KSWPDYLIPATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEAL-ISAD 240
           KS  D L+P +VK ++++F +A +K    P   FK ++E MI +RKERAELLEAL IS  
Sbjct: 181 KSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISNK 240

Query: 241 DFTITRYPQRMLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNK 300
           D TI R+ Q++ ++WGE+D +F+L+ A  MKE++G    ++ I+KAGH+  +ERPC+YN+
Sbjct: 241 DVTIPRFQQKIHLLWGESDQIFNLEFAKSMKEQLGENATMESIKKAGHLAHLERPCVYNR 300

Query: 301 CLQKFLHSLLEED 303
            L+KFL S+  E+
Sbjct: 301 RLKKFLASVYSEN 313

BLAST of MS020536 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.3e-80
Identity = 148/311 (47.59%), Postives = 209/311 (67.20%), Query Frame = 0

Query: 1   MVNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVP-------TRNPDPNPNPNPNI 60
           MVN V +    L   MK AGV P  ++IEPGT +NFWVP       +    P     P  
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 61  PLVLL-HGFAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKG 120
           P+VLL HGFA   I++WQFQV AL++ Y VY PD +FFG S TD  +RSP FQA+C+VKG
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 121 LRKLGVERCVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKS 180
           LR LGV++ V VGFSYGGMV F++AE +P+ V A+VV+GS   +T++++  SL  +G+ S
Sbjct: 121 LRILGVDKFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFSS 180

Query: 181 WPDYLIPATVKDVRSMFEIASYKLPPLPDWFFKHYLEAMIIHRKERAELLEA-LISADDF 240
             D L+P +V  ++++F IA +K    P   FK Y+E M  +RKERAELLEA ++S  + 
Sbjct: 181 STDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKEA 240

Query: 241 TITRYPQRMLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCL 300
            I  +P+++  +WGE+D +FDL++A  MKE++G    ++ I+KAGH+V +ERPC+YN+ L
Sbjct: 241 QIPHFPRKIHFLWGESDQIFDLELARDMKEQIGENATIESIKKAGHLVQLERPCVYNRRL 300

Query: 301 QKFLHSLLEED 303
           +KFL S+  ED
Sbjct: 301 KKFLASIHSED 311

BLAST of MS020536 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 4.3e-42
Identity = 98/289 (33.91%), Postives = 157/289 (54.33%), Query Frame = 0

Query: 17  KAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHGFAANAILSWQFQVLAL 76
           K +G+RP  + ++ GT +NFWV    P+  P PN    L+L+HG  A AI  W      L
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPN----LLLIHGLGATAIWQWYDVARRL 79

Query: 77  ARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVERCVLVGFSYGGMVGFRL 136
           +R +++Y PD +FFG S T RPERS  FQA+ +++ L    V++  LVG SYGG VG+R+
Sbjct: 80  SRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSYGGFVGYRM 139

Query: 137 AEIWPEFVAAMVVTGSPAALTE-SLSRGSLEEIGYKSWPDYLIPATVKDVRSMFEIASYK 196
           A ++ + V  +V+  +   + E  +  G  +          L+P +VK +R +     YK
Sbjct: 140 ASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYK 199

Query: 197 LPPL----PDWFFKHYLEAMII--HRKERAELLEALISADDFTITRYP---QRMLIIWGE 256
            P L    P      ++E  +   + +E+ EL++A+    D  I+  P   Q  LIIWGE
Sbjct: 200 -PALARLVPTCLLHDFIEHALTRDNMEEKRELIKAI--PKDRIISEIPKLKQPTLIIWGE 259

Query: 257 NDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFL 296
           +D +F L++  ++++ VG   K+  I++ GHI   E+P  + K L+ FL
Sbjct: 260 HDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFL 301

BLAST of MS020536 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 8.0e-41
Identity = 99/299 (33.11%), Postives = 160/299 (53.51%), Query Frame = 0

Query: 2   VNIVHLYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIP-LVLLHG 61
           +++       LR    +AG+  + + I+  T + FW P   P  + + N   P L+LLHG
Sbjct: 6   LSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGP---PPSSSSENTQKPSLLLLHG 65

Query: 62  FAANAILSWQFQVLALARNYDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVER 121
           F  +A+  W  QV  L+  + +Y PD +FFG S +    RS  FQA C+ K + KL VER
Sbjct: 66  FGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVER 125

Query: 122 CVLVGFSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIPA 181
             +VG SYGG V + +A+++PE V  +V+  S   L  S +   +         + ++PA
Sbjct: 126 FSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPA 185

Query: 182 TVKDVRSMF-EIASYKLPPLPDWFFKHYLEAMIIH-RKERAELLEAL-ISADDFT-ITRY 241
           +  D+R     ++S +L  +PD+    + + M    R+E+AELLE L I  DD T ++  
Sbjct: 186 SATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSPI 245

Query: 242 PQRMLIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFL 296
            Q +++IWGE D +F L++A+ +KE +G K  +  I+K  HI   E+   +N  +  FL
Sbjct: 246 QQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFL 301

BLAST of MS020536 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-39
Identity = 87/295 (29.49%), Postives = 167/295 (56.61%), Query Frame = 0

Query: 7   LYNRFLRWPMKAAGVRPELVQIEPGTAMNFWVPTRNPDPNPNPNPNIPLVLLHGFAANAI 66
           L   +LR  ++AAG+  + + I+  T ++FW P   P  + + +    ++LLHGF  +++
Sbjct: 12  LLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPP--PLDHRSDDDRPVMLLLHGFGPSSM 71

Query: 67  LSWQFQVLALARN-YDVYAPDFIFFGDSVTDRPERSPEFQAECVVKGLRKLGVERCVLVG 126
             W+ Q+ A + + + VY+PD +FFGDS +    R+  FQAEC+ K + K+G+ +  + G
Sbjct: 72  WQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAG 131

Query: 127 FSYGGMVGFRLAEIWPEFVAAMVVTGSPAALTESLSRGSLEEIGYKSWPDYLIPATVKDV 186
            SYGG V + +A++WPE V  +V+  S   + +      L+    +     ++P+T  + 
Sbjct: 132 TSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVMLPSTATEF 191

Query: 187 RSMFEIA-SYKLPPL-PDWFFKHYLEAMI-IHRKERAELLEALI--SADDFTITRYPQRM 246
           R++  +A S++L  + PD  +   +  +   +RKE+ ELL+ +    +++  I    Q +
Sbjct: 192 RTLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEV 251

Query: 247 LIIWGENDNLFDLQIAYKMKEKVGGKVKVDCIEKAGHIVAMERPCIYNKCLQKFL 296
           LI+WG+ D +F +++AY++KE +G K K++ I+   H+  +E    +N  + +FL
Sbjct: 252 LIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304