MS020444 (gene) Bitter gourd (TR) v1

BLAST of MS020444 vs. NCBI nr
Match: XP_022947209.1 (protein SICKLE isoform X2 [Cucurbita moschata])

HSP 1 Score: 488.8 bits (1257), Expect = 3.9e-134
Identity = 261/354 (73.73%), Postives = 287/354 (81.07%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYIETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPTHQFH 120
           YTNPMAAFS++KKRG   N  VS +YVPSH N+SP  +VPSNF G+RNPEMSPS  HQFH
Sbjct: 61  YTNPMAAFSSSKKRG---NQTVSHDYVPSHLNSSPDAYVPSNFPGMRNPEMSPSAPHQFH 120

Query: 121 QHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPRGMSS 180
           QHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FPG PPRGM+S
Sbjct: 121 QHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFPGPPPRGMNS 180

Query: 181 PRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGYEGSPSPGRGS 240
           PR PFVN FPS  PRDMSSPS V GPRGNSY N   D VN+ +PSPS GY+GS SPG GS
Sbjct: 181 PRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPNQTHDMVNHLSPSPSPGYQGSSSPGGGS 240

Query: 241 HGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDLQPGIWRTT 300
           HG RGNMTP+ RFGSGRG  SHGR FSS++S RPE   FYN SML+DPWK LQPGIWR  
Sbjct: 241 HGHRGNMTPSPRFGSGRGFGSHGRRFSSDESSRPEQ--FYNPSMLEDPWKVLQPGIWRPV 300

Query: 301 A----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVDDAP 350
           A     AN SESWISK   KKARVS+  S RS SQPSLAEYLAASFNEA ++ P
Sbjct: 301 APLRSSANPSESWISKFNTKKARVSDASSGRSGSQPSLAEYLAASFNEAANNTP 348

BLAST of MS020444 vs. NCBI nr
Match: XP_023533680.1 (protein SICKLE [Cucurbita pepo subsp. pepo] >XP_023533681.1 protein SICKLE [Cucurbita pepo subsp. pepo] >XP_023533682.1 protein SICKLE [Cucurbita pepo subsp. pepo] >XP_023533683.1 protein SICKLE [Cucurbita pepo subsp. pepo] >XP_023533684.1 protein SICKLE [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.6 bits (1246), Expect = 7.4e-133
Identity = 262/363 (72.18%), Postives = 289/363 (79.61%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYVETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGN-------FDNH--GVSDNYVPSHHNNSPATFVPSNFAGLRNPEM 120
           YTNPMAAFS++KKRGN         +H     D YVPSH N+SP  +VPSNF G+RNPEM
Sbjct: 61  YTNPMAAFSSSKKRGNQTVSHDYVPSHLNSSPDVYVPSHLNSSPDVYVPSNFPGMRNPEM 120

Query: 121 SPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFP 180
           SPS  HQFHQHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FP
Sbjct: 121 SPSAPHQFHQHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFP 180

Query: 181 GHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGYE 240
           G PPRGM+SPR PFVN FPS  PRDM SPS V GPRGNSY N  QD VN+ +PSPS GY+
Sbjct: 181 GPPPRGMNSPRGPFVNQFPSQLPRDMISPSFVSGPRGNSYPNQTQDMVNHLSPSPSPGYQ 240

Query: 241 GSPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKD 300
           GS SPG GSHG RGNMTP+ RFGSGRG  SHGR FSS++S RPE   FYN SML+DPWK 
Sbjct: 241 GSSSPGGGSHGHRGNMTPSPRFGSGRGFGSHGRRFSSDESSRPEQ--FYNPSMLEDPWKV 300

Query: 301 LQPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVD 350
           LQPGIWR  A     AN+SESWISK   KKARVS+  S RSSSQPSLAEYLAASFNEA +
Sbjct: 301 LQPGIWRPVAPLRSSANTSESWISKFNTKKARVSDASSGRSSSQPSLAEYLAASFNEAAN 360

BLAST of MS020444 vs. NCBI nr
Match: XP_022148514.1 (M-phase-specific PLK1-interacting protein [Momordica charantia])

HSP 1 Score: 483.0 bits (1242), Expect = 2.1e-132
Identity = 235/241 (97.51%), Postives = 237/241 (98.34%), Query Frame = 0

Query: 111 MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQF 170
           MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMD+GTPGIWSGPRSPFVNQF
Sbjct: 1   MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDRGTPGIWSGPRSPFVNQF 60

Query: 171 PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY 230
           PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY
Sbjct: 61  PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY 120

Query: 231 EGSPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWK 290
           EGSPSPGRGSHGF GNMTPTSRFGSGRGSS HGRHFSSNKS RPEHFPFYNESMLDDPWK
Sbjct: 121 EGSPSPGRGSHGFCGNMTPTSRFGSGRGSSYHGRHFSSNKSLRPEHFPFYNESMLDDPWK 180

Query: 291 DLQPGIWRTTARANSSESWISKSRMKKARVSEPFSRSSSQPSLAEYLAASFNEAVDDAPS 350
           DLQPGIWRTTARANSSESWIS+SRMKKARVSEPFS SSSQPSLAEYLAASFNEAVDDAPS
Sbjct: 181 DLQPGIWRTTARANSSESWISRSRMKKARVSEPFSSSSSQPSLAEYLAASFNEAVDDAPS 240

Query: 351 V 352
           V
Sbjct: 241 V 241

BLAST of MS020444 vs. NCBI nr
Match: XP_022947202.1 (protein SICKLE isoform X1 [Cucurbita moschata] >XP_022947203.1 protein SICKLE isoform X1 [Cucurbita moschata] >XP_022947205.1 protein SICKLE isoform X1 [Cucurbita moschata] >XP_022947206.1 protein SICKLE isoform X1 [Cucurbita moschata] >XP_022947207.1 protein SICKLE isoform X1 [Cucurbita moschata] >XP_022947208.1 protein SICKLE isoform X1 [Cucurbita moschata])

HSP 1 Score: 482.6 bits (1241), Expect = 2.8e-132
Identity = 261/363 (71.90%), Postives = 287/363 (79.06%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYIETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGN-------FDNH--GVSDNYVPSHHNNSPATFVPSNFAGLRNPEM 120
           YTNPMAAFS++KKRGN         +H     D YVPSH N+SP  +VPSNF G+RNPEM
Sbjct: 61  YTNPMAAFSSSKKRGNQTVSHDYVPSHLNSSPDAYVPSHLNSSPDVYVPSNFPGMRNPEM 120

Query: 121 SPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFP 180
           SPS  HQFHQHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FP
Sbjct: 121 SPSAPHQFHQHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFP 180

Query: 181 GHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGYE 240
           G PPRGM+SPR PFVN FPS  PRDMSSPS V GPRGNSY N   D VN+ +PSPS GY+
Sbjct: 181 GPPPRGMNSPRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPNQTHDMVNHLSPSPSPGYQ 240

Query: 241 GSPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKD 300
           GS SPG GSHG RGNMTP+ RFGSGRG  SHGR FSS++S RPE   FYN SML+DPWK 
Sbjct: 241 GSSSPGGGSHGHRGNMTPSPRFGSGRGFGSHGRRFSSDESSRPEQ--FYNPSMLEDPWKV 300

Query: 301 LQPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVD 350
           LQPGIWR  A     AN SESWISK   KKARVS+  S RS SQPSLAEYLAASFNEA +
Sbjct: 301 LQPGIWRPVAPLRSSANPSESWISKFNTKKARVSDASSGRSGSQPSLAEYLAASFNEAAN 360

BLAST of MS020444 vs. NCBI nr
Match: XP_023007497.1 (protein SICKLE isoform X1 [Cucurbita maxima] >XP_023007498.1 protein SICKLE isoform X1 [Cucurbita maxima] >XP_023007499.1 protein SICKLE isoform X1 [Cucurbita maxima])

HSP 1 Score: 469.9 bits (1208), Expect = 1.9e-128
Identity = 257/360 (71.39%), Postives = 283/360 (78.61%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 6   MEESEKRRERLRAMRMEASQADVANYVETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 65

Query: 61  YTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPTHQFH 120
           YTNPMAAFS++KKRG   N  VS  YVPSH N+SP  +VPSNF G+RNPEMSPS  HQFH
Sbjct: 66  YTNPMAAFSSSKKRG---NQTVSHAYVPSHLNSSPDAYVPSNFPGMRNPEMSPSAPHQFH 125

Query: 121 QHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPRGMSS 180
           QHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FPG PPR M+S
Sbjct: 126 QHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFPGPPPRVMNS 185

Query: 181 PRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSP--------SSGYEG 240
           PR PFVN FPS  PRDMSSPS V GPRGNSY +  QD VN+ +PSP        S GY+G
Sbjct: 186 PRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPSQTQDMVNHLSPSPSPGYQGSSSPGYQG 245

Query: 241 SPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
           S SPG GSHG RGNMTP+ RFG GRG  SHGR FSS+KS RPE   FY+ SML+DPWK L
Sbjct: 246 SSSPGGGSHGHRGNMTPSPRFGLGRGFGSHGRRFSSDKSSRPEQ--FYDPSMLEDPWKVL 305

Query: 301 QPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVDD 348
           QPGIWR  A     AN SESWISK   KKARV +  S RS SQPSLAEYLAASFNEA ++
Sbjct: 306 QPGIWRPVAPLRSSANPSESWISKFNTKKARVLDASSGRSGSQPSLAEYLAASFNEAANN 359

BLAST of MS020444 vs. ExPASy Swiss-Prot
Match: Q9SB47 (Protein SICKLE OS=Arabidopsis thaliana OX=3702 GN=SIC PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 129/364 (35.44%), Postives = 169/364 (46.43%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQAN---VGNYAETSL-PNHLSNPLVESSAAMLEQSEPCTAP 60
           ME+SEKR++ L+AMRMEAA  N        ETS+   HLSNPL E+S     Q +     
Sbjct: 1   MEDSEKRKQMLKAMRMEAAAQNDDDATTGTETSMSTGHLSNPLAETSN---HQQDSFETQ 60

Query: 61  RFDYYTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPT 120
           RFDYYT+PMAA+S+ KK        +S    PSH  +SP   VP  F     P + P   
Sbjct: 61  RFDYYTDPMAAYSSFKKNKTPKQQYISS---PSHQGSSP---VPPQFP----PSVPPGSL 120

Query: 121 HQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPR 180
              +Q   +   FHA  +   G      +    P  +G P  W+       N F      
Sbjct: 121 CSEYQAQTNHGGFHAAHYEPRG------MAHLSPSHRGPPAGWN-------NNF------ 180

Query: 181 GMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNY-HTPSPSSGYEGSPS 240
                R P VNH  S PP+ +  P        N   N    R +Y +TP   S Y     
Sbjct: 181 -----RPPPVNH--SGPPQWVPRPFPFSQEMPNMGNNRFGGRGSYNNTPPQFSNY----- 240

Query: 241 PGRGSHGFRGNMTPTSRFGSGRG---SSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
            GR +  + GN  P S  G  RG   ++S GR     +   P    FY+ SM +DPWK L
Sbjct: 241 -GRQNANWGGNTYPNSGRGRSRGRGMNTSFGRD-GGRRPMEPGAERFYSNSMAEDPWKHL 300

Query: 301 QPGIWRTTARANSSES----WISKS-RMKKARVSEPFSR-SSSQPSLAEYLAASFNEAVD 351
           +P +W+  + A+SS S    W+ KS   KK+  SE   + SS+Q SLAEYLAAS + A  
Sbjct: 301 KPVLWKNCSDASSSSSTGQAWLPKSIAPKKSVTSEATHKTSSNQQSLAEYLAASLDGATC 318

BLAST of MS020444 vs. ExPASy TrEMBL
Match: A0A6J1G649 (protein SICKLE isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451145 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.9e-134
Identity = 261/354 (73.73%), Postives = 287/354 (81.07%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYIETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPTHQFH 120
           YTNPMAAFS++KKRG   N  VS +YVPSH N+SP  +VPSNF G+RNPEMSPS  HQFH
Sbjct: 61  YTNPMAAFSSSKKRG---NQTVSHDYVPSHLNSSPDAYVPSNFPGMRNPEMSPSAPHQFH 120

Query: 121 QHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPRGMSS 180
           QHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FPG PPRGM+S
Sbjct: 121 QHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFPGPPPRGMNS 180

Query: 181 PRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGYEGSPSPGRGS 240
           PR PFVN FPS  PRDMSSPS V GPRGNSY N   D VN+ +PSPS GY+GS SPG GS
Sbjct: 181 PRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPNQTHDMVNHLSPSPSPGYQGSSSPGGGS 240

Query: 241 HGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDLQPGIWRTT 300
           HG RGNMTP+ RFGSGRG  SHGR FSS++S RPE   FYN SML+DPWK LQPGIWR  
Sbjct: 241 HGHRGNMTPSPRFGSGRGFGSHGRRFSSDESSRPEQ--FYNPSMLEDPWKVLQPGIWRPV 300

Query: 301 A----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVDDAP 350
           A     AN SESWISK   KKARVS+  S RS SQPSLAEYLAASFNEA ++ P
Sbjct: 301 APLRSSANPSESWISKFNTKKARVSDASSGRSGSQPSLAEYLAASFNEAANNTP 348

BLAST of MS020444 vs. ExPASy TrEMBL
Match: A0A6J1D482 (M-phase-specific PLK1-interacting protein OS=Momordica charantia OX=3673 GN=LOC111017142 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.0e-132
Identity = 235/241 (97.51%), Postives = 237/241 (98.34%), Query Frame = 0

Query: 111 MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQF 170
           MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMD+GTPGIWSGPRSPFVNQF
Sbjct: 1   MSPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDRGTPGIWSGPRSPFVNQF 60

Query: 171 PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY 230
           PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY
Sbjct: 61  PGHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGY 120

Query: 231 EGSPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWK 290
           EGSPSPGRGSHGF GNMTPTSRFGSGRGSS HGRHFSSNKS RPEHFPFYNESMLDDPWK
Sbjct: 121 EGSPSPGRGSHGFCGNMTPTSRFGSGRGSSYHGRHFSSNKSLRPEHFPFYNESMLDDPWK 180

Query: 291 DLQPGIWRTTARANSSESWISKSRMKKARVSEPFSRSSSQPSLAEYLAASFNEAVDDAPS 350
           DLQPGIWRTTARANSSESWIS+SRMKKARVSEPFS SSSQPSLAEYLAASFNEAVDDAPS
Sbjct: 181 DLQPGIWRTTARANSSESWISRSRMKKARVSEPFSSSSSQPSLAEYLAASFNEAVDDAPS 240

Query: 351 V 352
           V
Sbjct: 241 V 241

BLAST of MS020444 vs. ExPASy TrEMBL
Match: A0A6J1G5T4 (protein SICKLE isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451145 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.4e-132
Identity = 261/363 (71.90%), Postives = 287/363 (79.06%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYIETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGN-------FDNH--GVSDNYVPSHHNNSPATFVPSNFAGLRNPEM 120
           YTNPMAAFS++KKRGN         +H     D YVPSH N+SP  +VPSNF G+RNPEM
Sbjct: 61  YTNPMAAFSSSKKRGNQTVSHDYVPSHLNSSPDAYVPSHLNSSPDVYVPSNFPGMRNPEM 120

Query: 121 SPSPTHQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFP 180
           SPS  HQFHQHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FP
Sbjct: 121 SPSAPHQFHQHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFP 180

Query: 181 GHPPRGMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSPSSGYE 240
           G PPRGM+SPR PFVN FPS  PRDMSSPS V GPRGNSY N   D VN+ +PSPS GY+
Sbjct: 181 GPPPRGMNSPRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPNQTHDMVNHLSPSPSPGYQ 240

Query: 241 GSPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKD 300
           GS SPG GSHG RGNMTP+ RFGSGRG  SHGR FSS++S RPE   FYN SML+DPWK 
Sbjct: 241 GSSSPGGGSHGHRGNMTPSPRFGSGRGFGSHGRRFSSDESSRPEQ--FYNPSMLEDPWKV 300

Query: 301 LQPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVD 350
           LQPGIWR  A     AN SESWISK   KKARVS+  S RS SQPSLAEYLAASFNEA +
Sbjct: 301 LQPGIWRPVAPLRSSANPSESWISKFNTKKARVSDASSGRSGSQPSLAEYLAASFNEAAN 360

BLAST of MS020444 vs. ExPASy TrEMBL
Match: A0A6J1KYW0 (protein SICKLE isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499971 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 9.1e-129
Identity = 257/360 (71.39%), Postives = 283/360 (78.61%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 6   MEESEKRRERLRAMRMEASQADVANYVETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 65

Query: 61  YTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPTHQFH 120
           YTNPMAAFS++KKRG   N  VS  YVPSH N+SP  +VPSNF G+RNPEMSPS  HQFH
Sbjct: 66  YTNPMAAFSSSKKRG---NQTVSHAYVPSHLNSSPDAYVPSNFPGMRNPEMSPSAPHQFH 125

Query: 121 QHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPRGMSS 180
           QHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FPG PPR M+S
Sbjct: 126 QHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFPGPPPRVMNS 185

Query: 181 PRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSP--------SSGYEG 240
           PR PFVN FPS  PRDMSSPS V GPRGNSY +  QD VN+ +PSP        S GY+G
Sbjct: 186 PRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPSQTQDMVNHLSPSPSPGYQGSSSPGYQG 245

Query: 241 SPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
           S SPG GSHG RGNMTP+ RFG GRG  SHGR FSS+KS RPE   FY+ SML+DPWK L
Sbjct: 246 SSSPGGGSHGHRGNMTPSPRFGLGRGFGSHGRRFSSDKSSRPEQ--FYDPSMLEDPWKVL 305

Query: 301 QPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVDD 348
           QPGIWR  A     AN SESWISK   KKARV +  S RS SQPSLAEYLAASFNEA ++
Sbjct: 306 QPGIWRPVAPLRSSANPSESWISKFNTKKARVLDASSGRSGSQPSLAEYLAASFNEAANN 359

BLAST of MS020444 vs. ExPASy TrEMBL
Match: A0A6J1L0Q3 (protein SICKLE isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499971 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 9.1e-129
Identity = 257/360 (71.39%), Postives = 283/360 (78.61%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQANVGNYAETSLPNHLSNPLVESSAAMLEQSEPCTAPRFDY 60
           MEESEKRRERLRAMRMEA+QA+V NY ETSLPNHLSNPLVESSA ML QSEPCTAPRFDY
Sbjct: 1   MEESEKRRERLRAMRMEASQADVANYVETSLPNHLSNPLVESSANMLGQSEPCTAPRFDY 60

Query: 61  YTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPTHQFH 120
           YTNPMAAFS++KKRG   N  VS  YVPSH N+SP  +VPSNF G+RNPEMSPS  HQFH
Sbjct: 61  YTNPMAAFSSSKKRG---NQTVSHAYVPSHLNSSPDAYVPSNFPGMRNPEMSPSAPHQFH 120

Query: 121 QHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPRGMSS 180
           QHSPDQR F+ARG++GSG HG PA+PRPFPMDQ +P +W GPRSP+VN FPG PPR M+S
Sbjct: 121 QHSPDQR-FYARGYSGSGGHGSPAMPRPFPMDQRSPNMWGGPRSPYVNHFPGPPPRVMNS 180

Query: 181 PRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNYHTPSP--------SSGYEG 240
           PR PFVN FPS  PRDMSSPS V GPRGNSY +  QD VN+ +PSP        S GY+G
Sbjct: 181 PRGPFVNQFPSQLPRDMSSPSFVSGPRGNSYPSQTQDMVNHLSPSPSPGYQGSSSPGYQG 240

Query: 241 SPSPGRGSHGFRGNMTPTSRFGSGRGSSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
           S SPG GSHG RGNMTP+ RFG GRG  SHGR FSS+KS RPE   FY+ SML+DPWK L
Sbjct: 241 SSSPGGGSHGHRGNMTPSPRFGLGRGFGSHGRRFSSDKSSRPEQ--FYDPSMLEDPWKVL 300

Query: 301 QPGIWRTTA----RANSSESWISKSRMKKARVSEPFS-RSSSQPSLAEYLAASFNEAVDD 348
           QPGIWR  A     AN SESWISK   KKARV +  S RS SQPSLAEYLAASFNEA ++
Sbjct: 301 QPGIWRPVAPLRSSANPSESWISKFNTKKARVLDASSGRSGSQPSLAEYLAASFNEAANN 354

BLAST of MS020444 vs. TAIR 10
Match: AT4G24500.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-25
Identity = 129/364 (35.44%), Postives = 169/364 (46.43%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQAN---VGNYAETSL-PNHLSNPLVESSAAMLEQSEPCTAP 60
           ME+SEKR++ L+AMRMEAA  N        ETS+   HLSNPL E+S     Q +     
Sbjct: 1   MEDSEKRKQMLKAMRMEAAAQNDDDATTGTETSMSTGHLSNPLAETSN---HQQDSFETQ 60

Query: 61  RFDYYTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPT 120
           RFDYYT+PMAA+S+ KK        +S    PSH  +SP   VP  F     P + P   
Sbjct: 61  RFDYYTDPMAAYSSFKKNKTPKQQYISS---PSHQGSSP---VPPQFP----PSVPPGSL 120

Query: 121 HQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPR 180
              +Q   +   FHA  +   G      +    P  +G P  W+       N F      
Sbjct: 121 CSEYQAQTNHGGFHAAHYEPRG------MAHLSPSHRGPPAGWN-------NNF------ 180

Query: 181 GMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNY-HTPSPSSGYEGSPS 240
                R P VNH  S PP+ +  P        N   N    R +Y +TP   S Y     
Sbjct: 181 -----RPPPVNH--SGPPQWVPRPFPFSQEMPNMGNNRFGGRGSYNNTPPQFSNY----- 240

Query: 241 PGRGSHGFRGNMTPTSRFGSGRG---SSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
            GR +  + GN  P S  G  RG   ++S GR     +   P    FY+ SM +DPWK L
Sbjct: 241 -GRQNANWGGNTYPNSGRGRSRGRGMNTSFGRD-GGRRPMEPGAERFYSNSMAEDPWKHL 300

Query: 301 QPGIWRTTARANSSES----WISKS-RMKKARVSEPFSR-SSSQPSLAEYLAASFNEAVD 351
           +P +W+  + A+SS S    W+ KS   KK+  SE   + SS+Q SLAEYLAAS + A  
Sbjct: 301 KPVLWKNCSDASSSSSTGQAWLPKSIAPKKSVTSEATHKTSSNQQSLAEYLAASLDGATC 318

BLAST of MS020444 vs. TAIR 10
Match: AT4G24500.3 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-25
Identity = 129/364 (35.44%), Postives = 169/364 (46.43%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQAN---VGNYAETSL-PNHLSNPLVESSAAMLEQSEPCTAP 60
           ME+SEKR++ L+AMRMEAA  N        ETS+   HLSNPL E+S     Q +     
Sbjct: 1   MEDSEKRKQMLKAMRMEAAAQNDDDATTGTETSMSTGHLSNPLAETSN---HQQDSFETQ 60

Query: 61  RFDYYTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPT 120
           RFDYYT+PMAA+S+ KK        +S    PSH  +SP   VP  F     P + P   
Sbjct: 61  RFDYYTDPMAAYSSFKKNKTPKQQYISS---PSHQGSSP---VPPQFP----PSVPPGSL 120

Query: 121 HQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPR 180
              +Q   +   FHA  +   G      +    P  +G P  W+       N F      
Sbjct: 121 CSEYQAQTNHGGFHAAHYEPRG------MAHLSPSHRGPPAGWN-------NNF------ 180

Query: 181 GMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNY-HTPSPSSGYEGSPS 240
                R P VNH  S PP+ +  P        N   N    R +Y +TP   S Y     
Sbjct: 181 -----RPPPVNH--SGPPQWVPRPFPFSQEMPNMGNNRFGGRGSYNNTPPQFSNY----- 240

Query: 241 PGRGSHGFRGNMTPTSRFGSGRG---SSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
            GR +  + GN  P S  G  RG   ++S GR     +   P    FY+ SM +DPWK L
Sbjct: 241 -GRQNANWGGNTYPNSGRGRSRGRGMNTSFGRD-GGRRPMEPGAERFYSNSMAEDPWKHL 300

Query: 301 QPGIWRTTARANSSES----WISKS-RMKKARVSEPFSR-SSSQPSLAEYLAASFNEAVD 351
           +P +W+  + A+SS S    W+ KS   KK+  SE   + SS+Q SLAEYLAAS + A  
Sbjct: 301 KPVLWKNCSDASSSSSTGQAWLPKSIAPKKSVTSEATHKTSSNQQSLAEYLAASLDGATC 318

BLAST of MS020444 vs. TAIR 10
Match: AT4G24500.2 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 80.5 bits (197), Expect = 3.0e-15
Identity = 115/364 (31.59%), Postives = 150/364 (41.21%), Query Frame = 0

Query: 1   MEESEKRRERLRAMRMEAAQAN---VGNYAETSL-PNHLSNPLVESSAAMLEQSEPCTAP 60
           ME+SEKR++ L+AMRMEAA  N        ETS+   HLSNPL E+S             
Sbjct: 1   MEDSEKRKQMLKAMRMEAAAQNDDDATTGTETSMSTGHLSNPLAETS------------- 60

Query: 61  RFDYYTNPMAAFSATKKRGNFDNHGVSDNYVPSHHNNSPATFVPSNFAGLRNPEMSPSPT 120
                                 NH        SH  +SP   VP  F     P + P   
Sbjct: 61  ----------------------NHQQDSFETQSHQGSSP---VPPQFP----PSVPPGSL 120

Query: 121 HQFHQHSPDQRMFHARGFNGSGCHGGPAIPRPFPMDQGTPGIWSGPRSPFVNQFPGHPPR 180
              +Q   +   FHA  +   G      +    P  +G P  W+       N F      
Sbjct: 121 CSEYQAQTNHGGFHAAHYEPRG------MAHLSPSHRGPPAGWN-------NNF------ 180

Query: 181 GMSSPRSPFVNHFPSHPPRDMSSPSIVPGPRGNSYTNPMQDRVNY-HTPSPSSGYEGSPS 240
                R P VNH  S PP+ +  P        N   N    R +Y +TP   S Y     
Sbjct: 181 -----RPPPVNH--SGPPQWVPRPFPFSQEMPNMGNNRFGGRGSYNNTPPQFSNY----- 240

Query: 241 PGRGSHGFRGNMTPTSRFGSGRG---SSSHGRHFSSNKSPRPEHFPFYNESMLDDPWKDL 300
            GR +  + GN  P S  G  RG   ++S GR     +   P    FY+ SM +DPWK L
Sbjct: 241 -GRQNANWGGNTYPNSGRGRSRGRGMNTSFGRD-GGRRPMEPGAERFYSNSMAEDPWKHL 289

Query: 301 QPGIWRTTARANSSES----WISKS-RMKKARVSEPFSR-SSSQPSLAEYLAASFNEAVD 351
           +P +W+  + A+SS S    W+ KS   KK+  SE   + SS+Q SLAEYLAAS + A  
Sbjct: 301 KPVLWKNCSDASSSSSTGQAWLPKSIAPKKSVTSEATHKTSSNQQSLAEYLAASLDGATC 289