Homology
BLAST of MS020393 vs. NCBI nr
Match:
XP_022154188.1 (ubiquitin carboxyl-terminal hydrolase 27 isoform X1 [Momordica charantia])
HSP 1 Score: 483.8 bits (1244), Expect = 1.9e-132
Identity = 291/548 (53.10%), Postives = 357/548 (65.15%), Query Frame = 0
Query: 3 SHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLTVS 62
S S++L+LVPGLQN GNNCFFNVI+Q+LASC FQ FL++LV +F++HE MSET LTVS
Sbjct: 54 SSSERLRLVPGLQNLGNNCFFNVILQALASCVFFQPFLQKLVEDFEVHEGMSETLPLTVS 113
Query: 63 LAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGY 122
LAA+LEELS+I+ ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEIS Y
Sbjct: 114 LAAILEELSSIYLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDY 173
Query: 123 NQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCSSQ 182
N + CSLVDV SNG RI+TS S NELERWQ LGP DG+LGSILTCQ+C+SQ
Sbjct: 174 NHLHQCSLVDVFASNG----RIITSRSKGDNELERWQSHFLGPFDGVLGSILTCQSCTSQ 233
Query: 183 ISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISL 242
ISLSFE+ NLPLS VL GDSTIV+GC LVDCLKQFF EK+ENY CSY W
Sbjct: 234 ISLSFESCHNLPLSPVLKGDSTIVAGCRLVDCLKQFFATEKVENYRCSYCWH-------- 293
Query: 243 LWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLI 302
+ +++ + ++ + S +LLH K W NK SCTL
Sbjct: 294 ---------NAAVKYLSAVGASEMEIEELMKCDGQDSCGCHRLLHLHKVPWSNKFSCTLK 353
Query: 303 QLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLGG 362
QL+IA CPKILCIH+ RVS+NVFGDL+K++GHI FPL +D+SPFM+ GVEI+DLE QLGG
Sbjct: 354 QLSIAHCPKILCIHMKRVSMNVFGDLLKLQGHISFPLSLDVSPFMLTGVEIKDLEGQLGG 413
Query: 363 QHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRIIL----QATIESVSISKER 422
QHS++K + +F L NG + G + +R L E V IS E
Sbjct: 414 QHSSNKKPSHGLDFFRMQFDPRMLKYING---ITGEDSYRTNLTLGESICSEQVGISTEE 473
Query: 423 HPSSQANSKPVKI------PHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRR 482
+SQ + P + +R IA +E+CLYRLVSVVEHFG GGGHYTVYRR
Sbjct: 474 TSTSQTGGGLETLLNMNLQPSSPESRTCQIAPSETCLYRLVSVVEHFGRPGGGHYTVYRR 533
Query: 483 VQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARA 538
VQ G QDL+ + VS +WF VSDSEV +VS+++VL A A
Sbjct: 534 VQ-----------GHPLGQDLNGHLQAVS-------TEWFRVSDSEVHIVSEEEVLAAEA 559
BLAST of MS020393 vs. NCBI nr
Match:
XP_004148458.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus])
HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-127
Identity = 288/552 (52.17%), Postives = 348/552 (63.04%), Query Frame = 0
Query: 1 GTSHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERM-SETFSL 60
G S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++ V EF+LHE M SE L
Sbjct: 50 GPSSSENLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPL 109
Query: 61 TVSLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEI 120
TVSLAALLEEL I ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEI
Sbjct: 110 TVSLAALLEELGEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEI 169
Query: 121 SGYNQPYLCSLVDVIGSNGRDINRIVTSSN-CRNELERWQHRSLGPCDGILGSILTCQTC 180
S +N P CSL DV+GSNG RI+TS N ELERWQ L P DGILGSILTCQ+C
Sbjct: 170 SDFNYPAQCSLTDVMGSNG----RIITSCNESPTELERWQGHFLVPFDGILGSILTCQSC 229
Query: 181 SSQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHD 240
SSQISLSFE+F NLP+S L GDSTIV GCTL+DCLKQFF EK+ENY CSY W
Sbjct: 230 SSQISLSFESFHNLPVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWH----- 289
Query: 241 ISLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSC 300
+ L++ + ++ + S +LLH K W NK S
Sbjct: 290 ------------NAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSF 349
Query: 301 TLIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQ 360
TL QL+IA CPKILCIHL RVS N+FGD K++GHI FPLI+DMSPFM+ GVEI+DLE Q
Sbjct: 350 TLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDMSPFMLTGVEIKDLEGQ 409
Query: 361 LGGQHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRIIL-----------QAT 420
LGGQHS++K + +F L NG + G + F + L + +
Sbjct: 410 LGGQHSSNKKPSHGLDFFRTQFDARMLKYING---LTGEDEFPMNLTSGEPICYDRFKGS 469
Query: 421 IESVSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYT 480
+E S S+ R S ++ +S TR I +E+ LYR+VSVV+HFG GGGHYT
Sbjct: 470 VEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIEPSETFLYRVVSVVQHFGKPGGGHYT 529
Query: 481 VYRRVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVL 538
VYRRVQDY QD D VS +WF+VSDSEV LVS+++VL
Sbjct: 530 VYRRVQDY-----------LLGQDCDTQSQDVS-------TEWFHVSDSEVHLVSEEEVL 559
BLAST of MS020393 vs. NCBI nr
Match:
XP_008457921.1 (PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo] >KAA0045908.1 ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo var. makuwa] >TYK13681.1 ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo var. makuwa])
HSP 1 Score: 465.7 bits (1197), Expect = 5.4e-127
Identity = 286/549 (52.09%), Postives = 349/549 (63.57%), Query Frame = 0
Query: 3 SHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERM-SETFSLTV 62
S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++ V EF+LHE M E LTV
Sbjct: 52 SSSENLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTV 111
Query: 63 SLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISG 122
SLAALLEEL A+ ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEIS
Sbjct: 112 SLAALLEELGAVSLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISD 171
Query: 123 YNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCSS 182
+N P CSL DV+GSNG RI+TS + ELERWQ L P DGILGSILTCQ+CSS
Sbjct: 172 FNYPAQCSLTDVMGSNG----RIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSS 231
Query: 183 QISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDIS 242
QISLSFE+F NLP+S VL GDSTIV GCTL+DCLKQFF EK+ENY CSY W
Sbjct: 232 QISLSFESFHNLPVSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWH------- 291
Query: 243 LLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTL 302
+ L++ + ++ + S +LLH K W NK S TL
Sbjct: 292 ----------NAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTL 351
Query: 303 IQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLG 362
QL+IA CPKILCIHL RVS N+FGD K++GHI FPLI+DMSPFM+ GVEI+DL QLG
Sbjct: 352 KQLSIAHCPKILCIHLKRVSTNMFGDTFKLQGHISFPLILDMSPFMLTGVEIKDL-GQLG 411
Query: 363 GQHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRII----------LQATIES 422
GQHS++K + +F L NG + G + F I+ ++ ++E
Sbjct: 412 GQHSSNKKSSHGLDFFRTQFDARMLKYING---LTGEDEFPILTSGESICYDRVKGSVEE 471
Query: 423 VSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYR 482
S S+ R S ++ +S TR I +E+CLYR+VSVV+HFG GGGHYTVYR
Sbjct: 472 TSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETCLYRVVSVVQHFGKPGGGHYTVYR 531
Query: 483 RVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGAR 538
RVQDY QD D VS +WF+VSDSEV LVS+++VL A
Sbjct: 532 RVQDY-----------LLGQDCDTQSQEVS-------TEWFHVSDSEVHLVSEEEVLAAE 557
BLAST of MS020393 vs. NCBI nr
Match:
XP_023513383.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 465.7 bits (1197), Expect = 5.4e-127
Identity = 285/548 (52.01%), Postives = 347/548 (63.32%), Query Frame = 0
Query: 1 GTSHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLT 60
G S S+ L+LVPGLQN GNNCFFNVI+Q+LASC FQ FL +L+ EF+LHE MS+ LT
Sbjct: 54 GASSSQNLRLVPGLQNLGNNCFFNVILQALASCVFFQPFLHKLMEEFELHEEMSDALPLT 113
Query: 61 VSLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEIS 120
+SLAALLEELSAI ++VV SP KVM AMA++APNF+LT QQDAAEAFL L+S LKEEIS
Sbjct: 114 ISLAALLEELSAISSERVVLSPRKVMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEIS 173
Query: 121 GYNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCS 180
YN P CSLVDV+ SNG RI+TS S +ELERWQ L P DGILGSILTCQ+CS
Sbjct: 174 DYNHPPQCSLVDVLASNG----RIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCS 233
Query: 181 SQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDI 240
SQISLSFE+F NLPLS VL GDSTIV GCTL+DCLKQFF EK+ENY CS W
Sbjct: 234 SQISLSFESFHNLPLSPVLRGDSTIVFGCTLMDCLKQFFAAEKVENYRCSSCWH------ 293
Query: 241 SLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCT 300
D L++ + ++ + S +L K W NK S T
Sbjct: 294 -----------DAGLKYLSSMGASKMEIEELMKCDGQDSCVCDRLFDLYKVPWSNKFSFT 353
Query: 301 LIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQL 360
L QL+IA CPKILCIHL RVS N+FGD +K++GHI FPLI+DMSPFM+ GVEI+D+E+QL
Sbjct: 354 LKQLSIAHCPKILCIHLKRVSTNMFGDALKLQGHISFPLILDMSPFMLTGVEIKDIEKQL 413
Query: 361 GGQHSASKVKQIQVWSKICEFCVIFLN--NGWM--------MVLGREWFRIILQATIESV 420
G QHS++K ++ +F LN NG + G + L+ + E
Sbjct: 414 GKQHSSNKKPSHRLDFFRRQFDTTMLNYINGLTGEDDYPTNLASGEPFCSNQLKPSAEET 473
Query: 421 SISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRR 480
S S+ R S +S TR I +E+ LYR+VSVV+HFG GGGHYTVYRR
Sbjct: 474 SSSETRASSETWDSLD--------TRTSQIEPSETFLYRVVSVVQHFGRAGGGHYTVYRR 533
Query: 481 VQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARA 538
VQD + R QD+ +WF+VSDSEV LVS+++VL A A
Sbjct: 534 VQDSDA----------RLQDVS--------------TEWFHVSDSEVHLVSEEEVLAAEA 548
BLAST of MS020393 vs. NCBI nr
Match:
XP_022928102.1 (ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita moschata])
HSP 1 Score: 464.2 bits (1193), Expect = 1.6e-126
Identity = 280/549 (51.00%), Postives = 349/549 (63.57%), Query Frame = 0
Query: 1 GTSHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLT 60
G S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++L+ EF+LHE MS+ LT
Sbjct: 54 GASSSQNLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLT 113
Query: 61 VSLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEIS 120
+SLAALLEELSAI ++VV SP K+M AMA++APNF+LT QQDAAEAFL L+S LKEEIS
Sbjct: 114 ISLAALLEELSAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEIS 173
Query: 121 GYNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCS 180
YN P CSLVDV+ SNG RI+TS S +ELERWQ L P DGILGSILTCQ+CS
Sbjct: 174 DYNHPPQCSLVDVLASNG----RIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCS 233
Query: 181 SQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDI 240
SQISLSFE+F NLPLS VL GDSTIV GCTL+DCLKQFF EK+ENY CS W
Sbjct: 234 SQISLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWH------ 293
Query: 241 SLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCT 300
D L++ + ++ + S +L K W NK S T
Sbjct: 294 -----------DAGLKYLSSMGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFT 353
Query: 301 LIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQL 360
L QL+IA CPKILCIHL RVS N+FGD++K++GHI FPLI+DMSPFM+ GVEI+D+E+QL
Sbjct: 354 LKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHISFPLILDMSPFMLTGVEIKDIEKQL 413
Query: 361 GGQHSASKVKQ--IQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATI---------ES 420
G QHS++K + V+ + + ++ NG + G + + L + + +
Sbjct: 414 GKQHSSNKKPSHGLDVFRRQFDTTMLNYING---LTGEDDYPTNLASGVPFCSKQLKPSA 473
Query: 421 VSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYR 480
V S SS + TR I +E+ LYR+VSVV+HFG GGGHYTVYR
Sbjct: 474 VETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYR 533
Query: 481 RVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGAR 538
RVQD + R QD+ +WF+VSDSEV LVS+++VL A
Sbjct: 534 RVQDSDA----------RLQDVS--------------TEWFHVSDSEVHLVSEEEVLAAE 554
BLAST of MS020393 vs. ExPASy Swiss-Prot
Match:
Q9FPS0 (Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana OX=3702 GN=UBP27 PE=2 SV=1)
HSP 1 Score: 256.5 bits (654), Expect = 6.6e-67
Identity = 196/536 (36.57%), Postives = 267/536 (49.81%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFK--LHERMSETFSLTVSLAALL 69
LVPGLQN GNNCF NVI+Q+LASC+ F+ FL+ ++ + + L E LT +L+ALL
Sbjct: 72 LVPGLQNLGNNCFLNVILQALASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALL 131
Query: 70 EELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQPYL 129
+EL + ++ VS+P KVM + +A NF+LT QQDAAEA L L+S L+EEI +P
Sbjct: 132 QELGTVGSRRSVSNPRKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQ 191
Query: 130 CS-LVDVIGSNGRDINRIVTSSNCRN--ELERWQHRSLGPCDGILGSILTCQTCSSQISL 189
S L D++ S R++ + S EL+RW GP DGILGS L C+TCSSQISL
Sbjct: 192 SSNLSDILFS--RNLRMLAPSEGLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISL 251
Query: 190 SFETFQNLPLS-LVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISLLW 249
F+ F LPLS L+ G I+SGCTL CLK+F + EK+ENY C +R WH +L +
Sbjct: 252 EFQFFHTLPLSPLLHHGGYNIMSGCTLEHCLKKFLNTEKVENYFC---YRC-WHGAALKY 311
Query: 250 WKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLIQL 309
+ + ++ KL + LH + W N S L QL
Sbjct: 312 LSVIGAAETEIE--KL-----------RSCGGEDQCDCKTSLHLQRMPWSNSYSHILKQL 371
Query: 310 TIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLGGQH 369
IAR PK+LCI + R S N+F + K+ GHI FPL++++S F
Sbjct: 372 IIARFPKLLCIQVQRASFNMFEEF-KLSGHIAFPLVLNLSLF------------------ 431
Query: 370 SASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSIS-KERHPSSQAN 429
T S+ ++ +ER S
Sbjct: 432 -----------------------------------------TPSSIGVNIEERIEMSSEY 491
Query: 430 SKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLKRKK 489
KP A+ +YRLV+VVEHFG G GHYTVYR V+ +S ++
Sbjct: 492 QKPE-------------ASKNHGMYRLVTVVEHFGRTGSGHYTVYRSVRVFSQEE----- 494
Query: 490 GKRRAQDLDWNFPTVSKDQDHDHD-QWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
+++D D D WF +SDSEV VS+ VLGA A LLFYE++
Sbjct: 552 ----------------EEEDCDEDLSWFSISDSEVCRVSESDVLGAEASLLFYERL 494
BLAST of MS020393 vs. ExPASy Swiss-Prot
Match:
A4QNN3 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis OX=8364 GN=usp30 PE=2 SV=1)
HSP 1 Score: 101.3 bits (251), Expect = 3.5e-20
Identity = 126/536 (23.51%), Postives = 204/536 (38.06%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLTVSLAALLEE 69
LVPGL N GN CF N ++Q LASC F +L +++ +E L+V+L LL+
Sbjct: 83 LVPGLLNLGNTCFMNSLLQGLASCPSFIRWLADFTSKYRQENNTTEHQHLSVTLLHLLKA 142
Query: 70 L---SAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQPY 129
L + + +SP ++ + H S +QDA E F L S L++E +P+
Sbjct: 143 LCNQEGTEDEVLDASP--LLEVLRAHRWQISSFEEQDAHELFHVLTSSLEDERD--RRPH 202
Query: 130 LCSLVDVIGSN-GRDINRIVTSSNCRNELERW----QHRSLGPCDGILGSILTCQTCSSQ 189
+ L D+ + R + +CR ++ + Q +S P G L S + C+ C Q
Sbjct: 203 VTHLFDLDSLEFPLEPQRQI---HCRTQVPIYPIPSQWKSQHPFHGRLTSNMVCKHCQHQ 262
Query: 190 ISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISL 249
+ ++TF +L LS+ + +T TL CL+ F E +++ C
Sbjct: 263 SPMRYDTFDSLSLSIPV---ATWGHPITLDQCLQHFISTESVKDVVC------------- 322
Query: 250 LWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLI 309
E I A++ + N+ + +
Sbjct: 323 --------------------------------ENCTKIHAAQIPNSQSV--ENRKTTFVK 382
Query: 310 QLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLGG 369
QL + + P+ LCIHL R+S + G +K H+ F + M F R +Q
Sbjct: 383 QLKLGKLPQCLCIHLQRLSWSNQGSPLKRNEHVQFSEFLAMDRFKYRISGCSTSKQPANH 442
Query: 370 QHSASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSISKERHPSSQA 429
+A + + FLN
Sbjct: 443 LSAAEQETTDGKEGGAQNPTMPFLNGA--------------------------------C 502
Query: 430 NSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLKRK 489
++ + P S + S L+RL++VV H G + GH+ YRR +QKL +
Sbjct: 503 STSYISPPFTSPLPTNPEWTSSSYLFRLMAVVVHHGDMHSGHFVTYRRSPAAKNQKLTSQ 504
Query: 490 KGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
QW ++SD VR + Q+VL + A LLFYE+I
Sbjct: 563 -------------------------QWLWISDDTVRRTNFQEVLSSSAYLLFYERI 504
BLAST of MS020393 vs. ExPASy Swiss-Prot
Match:
Q3UN04 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus OX=10090 GN=Usp30 PE=1 SV=1)
HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 134/539 (24.86%), Postives = 211/539 (39.15%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFS-LTVSLAALLE 69
LVPGL N GN CF N ++Q L++C F +L ++ ++ T L+++L LL+
Sbjct: 66 LVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLNLLK 125
Query: 70 ELSA--IHPKKVV--SSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQ 129
LS + +V+ S V+R +F +QDA E F + S L++E Q
Sbjct: 126 ALSCQEVTEDEVLDASCLLDVLRMYRWQISSFE---EQDAHELFHVITSSLEDERD--RQ 185
Query: 130 PYLCSLVDV--IGSNGRDINRIVTSSNCRNELERWQH-RSLGPCDGILGSILTCQTCSSQ 189
P + L DV + R VT + H +S P G L S + C+ C Q
Sbjct: 186 PRVTHLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNHWKSQHPFHGRLTSNMVCKHCEHQ 245
Query: 190 ISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISL 249
+ F+TF +L LS+ +T TL CL F E + + C +
Sbjct: 246 SPVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCDNCTK-------- 305
Query: 250 LWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLI 309
E +++ K+ H + S +
Sbjct: 306 -------------------------------IEARGTLTGEKVEH--------QRSTFVK 365
Query: 310 QLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFP--LIMDMSPFMMRGVE-IRDLEQQ 369
QL + + P+ LCIHL R+S + G +K H+ F L+MD + + G + + +
Sbjct: 366 QLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDFYKYRLLGHKPSQHGPKA 425
Query: 370 LGGQHSASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSISKERHPS 429
SA +V+ Q K + G +I L + PS
Sbjct: 426 TENPGSAPEVQDAQAAPK-----------PGLSQPGAPKTQIFLNGACSPSLL--PALPS 485
Query: 430 SQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKL 489
A PV ++S+T L+RL++VV H G + GH+ YRR
Sbjct: 486 PVAFPLPVVPDYSSST----------YLFRLMAVVVHHGDMHSGHFVTYRR--------- 502
Query: 490 KRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
P +K+ +QW ++SD VR S Q+VL + A LLFYE++
Sbjct: 546 ---------------SPPSAKNPLSTSNQWLWISDDTVRKASLQEVLSSSAYLLFYERV 502
BLAST of MS020393 vs. ExPASy Swiss-Prot
Match:
D3ZPG5 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus OX=10116 GN=Usp30 PE=3 SV=1)
HSP 1 Score: 81.3 bits (199), Expect = 3.8e-14
Identity = 135/547 (24.68%), Postives = 211/547 (38.57%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFS-LTVSLAALLE 69
LVPGL N GN CF N ++Q L++C F +L ++ ++ T L+++L +LL+
Sbjct: 66 LVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLSLLK 125
Query: 70 ELSA--IHPKKVV--SSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQ 129
LS + ++V+ S V+R +F +QDA E F + S L++E Q
Sbjct: 126 ALSCQEVTEEEVLDASCLLDVLRMYRWQISSFE---EQDAHELFHVITSSLEDERD--RQ 185
Query: 130 PYLCSLVDV--IGSNGRDINRIV---TSSNCRNELERWQHRSLGPCDGILGSILTCQTCS 189
P + L DV + R V T + W +S P G L S + C+ C
Sbjct: 186 PRVTHLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNPW--KSQHPFHGRLSSNMVCKHCE 245
Query: 190 SQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDI 249
Q + F+TF +L LS+ +T TL CL F E + + C +
Sbjct: 246 HQSPVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCD--------NC 305
Query: 250 SLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCT 309
+ + K Q K+ + F++
Sbjct: 306 TKIEAKGTQ------NGEKVEHQRTTFVK------------------------------- 365
Query: 310 LIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFP--LIMDMSPFMMRGVE-IRDLE 369
QL + + P+ LCIHL R+S + G +K H+ F L+MD + + G + +
Sbjct: 366 --QLKLGKLPQCLCIHLQRLSWSSQGTPLKRHEHVQFNEFLMMDFYKYRLLGHKPSQHGP 425
Query: 370 QQLGGQHSASKVKQIQVWSK------ICEFCVIFLNNGWMMVLGREWFRIILQATIESVS 429
+ SA V+ Q K F+N L L A
Sbjct: 426 KATESPGSALGVQDTQAAPKPGLSQPAAPKTQFFMNGACSPSL--------LPAL----- 485
Query: 430 ISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRV 489
PS A PV ++S+ L+RL++VV H G + GH+ YRR
Sbjct: 486 ------PSPMAFPLPVAPDYSSS----------MYLFRLMAVVVHHGDMHSGHFVTYRR- 502
Query: 490 QDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARAM 538
P +K+ +QW ++SD VR S Q+VL + A
Sbjct: 546 -----------------------SPPSAKNPLSTSNQWLWISDDTVRKASLQEVLSSSAY 502
BLAST of MS020393 vs. ExPASy Swiss-Prot
Match:
Q70CQ3 (Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens OX=9606 GN=USP30 PE=1 SV=1)
HSP 1 Score: 79.7 bits (195), Expect = 1.1e-13
Identity = 127/538 (23.61%), Postives = 208/538 (38.66%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFS-LTVSLAALLE 69
LVPGL N GN CF N ++Q L++C F +L ++ ++ + L+++L LL+
Sbjct: 66 LVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLK 125
Query: 70 ELSA--IHPKKVV--SSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQ 129
LS + +V+ S V+R +F +QDA E F + S L++E Q
Sbjct: 126 ALSCQEVTDDEVLDASCLLDVLRMYRWQISSFE---EQDAHELFHVITSSLEDERD--RQ 185
Query: 130 PYLCSLVDV--IGSNGRDINRIVTSSNCRNELERWQH-RSLGPCDGILGSILTCQTCSSQ 189
P + L DV + + +T + H +S P G L S + C+ C Q
Sbjct: 186 PRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFHGRLTSNMVCKHCEHQ 245
Query: 190 ISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISL 249
+ F+TF +L LS+ +T TL CL F E + + C + +
Sbjct: 246 SPVRFDTFDSLSLSIPA---ATWGHPLTLDHCLHHFISSESVRDVVCDNCTKIEAKGT-- 305
Query: 250 LWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLI 309
L K+ + F++
Sbjct: 306 ------------LNGEKVEHQRTTFVK--------------------------------- 365
Query: 310 QLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFP--LIMDMSPFMMRGVEIRDLEQQL 369
QL + + P+ LCIHL R+S + G +K H+ F L+MD+ + + G + +L
Sbjct: 366 QLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKL 425
Query: 370 GGQHSASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSISKERHPSS 429
+ Q + LN G +I + + S P+
Sbjct: 426 NKNPGPTLELQDGPGAP-----TPVLNQP-----GAPKTQIFMNG-----ACSPSLLPTL 485
Query: 430 QANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLK 489
A P +P V ++ + L+RL++VV H G + GH+ YRR
Sbjct: 486 SA-PMPFPLP------VVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRR---------- 502
Query: 490 RKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
P +++ +QW +VSD VR S Q+VL + A LLFYE++
Sbjct: 546 --------------SPPSARNPLSTSNQWLWVSDDTVRKASLQEVLSSSAYLLFYERV 502
BLAST of MS020393 vs. ExPASy TrEMBL
Match:
A0A6J1DJL7 (Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC111021505 PE=3 SV=1)
HSP 1 Score: 483.8 bits (1244), Expect = 9.3e-133
Identity = 291/548 (53.10%), Postives = 357/548 (65.15%), Query Frame = 0
Query: 3 SHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLTVS 62
S S++L+LVPGLQN GNNCFFNVI+Q+LASC FQ FL++LV +F++HE MSET LTVS
Sbjct: 54 SSSERLRLVPGLQNLGNNCFFNVILQALASCVFFQPFLQKLVEDFEVHEGMSETLPLTVS 113
Query: 63 LAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGY 122
LAA+LEELS+I+ ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEIS Y
Sbjct: 114 LAAILEELSSIYLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDY 173
Query: 123 NQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCSSQ 182
N + CSLVDV SNG RI+TS S NELERWQ LGP DG+LGSILTCQ+C+SQ
Sbjct: 174 NHLHQCSLVDVFASNG----RIITSRSKGDNELERWQSHFLGPFDGVLGSILTCQSCTSQ 233
Query: 183 ISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISL 242
ISLSFE+ NLPLS VL GDSTIV+GC LVDCLKQFF EK+ENY CSY W
Sbjct: 234 ISLSFESCHNLPLSPVLKGDSTIVAGCRLVDCLKQFFATEKVENYRCSYCWH-------- 293
Query: 243 LWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLI 302
+ +++ + ++ + S +LLH K W NK SCTL
Sbjct: 294 ---------NAAVKYLSAVGASEMEIEELMKCDGQDSCGCHRLLHLHKVPWSNKFSCTLK 353
Query: 303 QLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLGG 362
QL+IA CPKILCIH+ RVS+NVFGDL+K++GHI FPL +D+SPFM+ GVEI+DLE QLGG
Sbjct: 354 QLSIAHCPKILCIHMKRVSMNVFGDLLKLQGHISFPLSLDVSPFMLTGVEIKDLEGQLGG 413
Query: 363 QHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRIIL----QATIESVSISKER 422
QHS++K + +F L NG + G + +R L E V IS E
Sbjct: 414 QHSSNKKPSHGLDFFRMQFDPRMLKYING---ITGEDSYRTNLTLGESICSEQVGISTEE 473
Query: 423 HPSSQANSKPVKI------PHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRR 482
+SQ + P + +R IA +E+CLYRLVSVVEHFG GGGHYTVYRR
Sbjct: 474 TSTSQTGGGLETLLNMNLQPSSPESRTCQIAPSETCLYRLVSVVEHFGRPGGGHYTVYRR 533
Query: 483 VQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGARA 538
VQ G QDL+ + VS +WF VSDSEV +VS+++VL A A
Sbjct: 534 VQ-----------GHPLGQDLNGHLQAVS-------TEWFRVSDSEVHIVSEEEVLAAEA 559
BLAST of MS020393 vs. ExPASy TrEMBL
Match:
A0A0A0LJP9 (USP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G298270 PE=3 SV=1)
HSP 1 Score: 468.0 bits (1203), Expect = 5.3e-128
Identity = 288/552 (52.17%), Postives = 348/552 (63.04%), Query Frame = 0
Query: 1 GTSHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERM-SETFSL 60
G S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++ V EF+LHE M SE L
Sbjct: 50 GPSSSENLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPL 109
Query: 61 TVSLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEI 120
TVSLAALLEEL I ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEI
Sbjct: 110 TVSLAALLEELGEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEI 169
Query: 121 SGYNQPYLCSLVDVIGSNGRDINRIVTSSN-CRNELERWQHRSLGPCDGILGSILTCQTC 180
S +N P CSL DV+GSNG RI+TS N ELERWQ L P DGILGSILTCQ+C
Sbjct: 170 SDFNYPAQCSLTDVMGSNG----RIITSCNESPTELERWQGHFLVPFDGILGSILTCQSC 229
Query: 181 SSQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHD 240
SSQISLSFE+F NLP+S L GDSTIV GCTL+DCLKQFF EK+ENY CSY W
Sbjct: 230 SSQISLSFESFHNLPVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWH----- 289
Query: 241 ISLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSC 300
+ L++ + ++ + S +LLH K W NK S
Sbjct: 290 ------------NAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSF 349
Query: 301 TLIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQ 360
TL QL+IA CPKILCIHL RVS N+FGD K++GHI FPLI+DMSPFM+ GVEI+DLE Q
Sbjct: 350 TLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDMSPFMLTGVEIKDLEGQ 409
Query: 361 LGGQHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRIIL-----------QAT 420
LGGQHS++K + +F L NG + G + F + L + +
Sbjct: 410 LGGQHSSNKKPSHGLDFFRTQFDARMLKYING---LTGEDEFPMNLTSGEPICYDRFKGS 469
Query: 421 IESVSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYT 480
+E S S+ R S ++ +S TR I +E+ LYR+VSVV+HFG GGGHYT
Sbjct: 470 VEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIEPSETFLYRVVSVVQHFGKPGGGHYT 529
Query: 481 VYRRVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVL 538
VYRRVQDY QD D VS +WF+VSDSEV LVS+++VL
Sbjct: 530 VYRRVQDY-----------LLGQDCDTQSQDVS-------TEWFHVSDSEVHLVSEEEVL 559
BLAST of MS020393 vs. ExPASy TrEMBL
Match:
A0A5A7TSR6 (Ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002010 PE=3 SV=1)
HSP 1 Score: 465.7 bits (1197), Expect = 2.6e-127
Identity = 286/549 (52.09%), Postives = 349/549 (63.57%), Query Frame = 0
Query: 3 SHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERM-SETFSLTV 62
S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++ V EF+LHE M E LTV
Sbjct: 52 SSSENLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTV 111
Query: 63 SLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISG 122
SLAALLEEL A+ ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEIS
Sbjct: 112 SLAALLEELGAVSLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISD 171
Query: 123 YNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCSS 182
+N P CSL DV+GSNG RI+TS + ELERWQ L P DGILGSILTCQ+CSS
Sbjct: 172 FNYPAQCSLTDVMGSNG----RIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSS 231
Query: 183 QISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDIS 242
QISLSFE+F NLP+S VL GDSTIV GCTL+DCLKQFF EK+ENY CSY W
Sbjct: 232 QISLSFESFHNLPVSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWH------- 291
Query: 243 LLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTL 302
+ L++ + ++ + S +LLH K W NK S TL
Sbjct: 292 ----------NAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTL 351
Query: 303 IQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLG 362
QL+IA CPKILCIHL RVS N+FGD K++GHI FPLI+DMSPFM+ GVEI+DL QLG
Sbjct: 352 KQLSIAHCPKILCIHLKRVSTNMFGDTFKLQGHISFPLILDMSPFMLTGVEIKDL-GQLG 411
Query: 363 GQHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRII----------LQATIES 422
GQHS++K + +F L NG + G + F I+ ++ ++E
Sbjct: 412 GQHSSNKKSSHGLDFFRTQFDARMLKYING---LTGEDEFPILTSGESICYDRVKGSVEE 471
Query: 423 VSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYR 482
S S+ R S ++ +S TR I +E+CLYR+VSVV+HFG GGGHYTVYR
Sbjct: 472 TSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETCLYRVVSVVQHFGKPGGGHYTVYR 531
Query: 483 RVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGAR 538
RVQDY QD D VS +WF+VSDSEV LVS+++VL A
Sbjct: 532 RVQDY-----------LLGQDCDTQSQEVS-------TEWFHVSDSEVHLVSEEEVLAAE 557
BLAST of MS020393 vs. ExPASy TrEMBL
Match:
A0A1S3C667 (ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo OX=3656 GN=LOC103497488 PE=3 SV=1)
HSP 1 Score: 465.7 bits (1197), Expect = 2.6e-127
Identity = 286/549 (52.09%), Postives = 349/549 (63.57%), Query Frame = 0
Query: 3 SHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERM-SETFSLTV 62
S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++ V EF+LHE M E LTV
Sbjct: 52 SSSENLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTV 111
Query: 63 SLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISG 122
SLAALLEEL A+ ++VV SP KVM AMA++ PNFSLT QQDAAEAFL L+S LKEEIS
Sbjct: 112 SLAALLEELGAVSLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISD 171
Query: 123 YNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCSS 182
+N P CSL DV+GSNG RI+TS + ELERWQ L P DGILGSILTCQ+CSS
Sbjct: 172 FNYPAQCSLTDVMGSNG----RIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSS 231
Query: 183 QISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDIS 242
QISLSFE+F NLP+S VL GDSTIV GCTL+DCLKQFF EK+ENY CSY W
Sbjct: 232 QISLSFESFHNLPVSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWH------- 291
Query: 243 LLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTL 302
+ L++ + ++ + S +LLH K W NK S TL
Sbjct: 292 ----------NAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTL 351
Query: 303 IQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLG 362
QL+IA CPKILCIHL RVS N+FGD K++GHI FPLI+DMSPFM+ GVEI+DL QLG
Sbjct: 352 KQLSIAHCPKILCIHLKRVSTNMFGDTFKLQGHISFPLILDMSPFMLTGVEIKDL-GQLG 411
Query: 363 GQHSASKVKQIQVWSKICEFCVIFLN--NGWMMVLGREWFRII----------LQATIES 422
GQHS++K + +F L NG + G + F I+ ++ ++E
Sbjct: 412 GQHSSNKKSSHGLDFFRTQFDARMLKYING---LTGEDEFPILTSGESICYDRVKGSVEE 471
Query: 423 VSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYR 482
S S+ R S ++ +S TR I +E+CLYR+VSVV+HFG GGGHYTVYR
Sbjct: 472 TSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETCLYRVVSVVQHFGKPGGGHYTVYR 531
Query: 483 RVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGAR 538
RVQDY QD D VS +WF+VSDSEV LVS+++VL A
Sbjct: 532 RVQDY-----------LLGQDCDTQSQEVS-------TEWFHVSDSEVHLVSEEEVLAAE 557
BLAST of MS020393 vs. ExPASy TrEMBL
Match:
A0A6J1EIY6 (ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita moschata OX=3662 GN=LOC111434991 PE=3 SV=1)
HSP 1 Score: 464.2 bits (1193), Expect = 7.6e-127
Identity = 280/549 (51.00%), Postives = 349/549 (63.57%), Query Frame = 0
Query: 1 GTSHSKKLQLVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFKLHERMSETFSLT 60
G S S+ L+L+PGLQN GNNCFFNVI+Q+LASC FQ FL++L+ EF+LHE MS+ LT
Sbjct: 54 GASSSQNLRLLPGLQNLGNNCFFNVILQALASCVFFQPFLQKLMEEFELHEEMSDALPLT 113
Query: 61 VSLAALLEELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEIS 120
+SLAALLEELSAI ++VV SP K+M AMA++APNF+LT QQDAAEAFL L+S LKEEIS
Sbjct: 114 ISLAALLEELSAISSERVVLSPRKIMLAMAYYAPNFNLTSQQDAAEAFLHLLSSLKEEIS 173
Query: 121 GYNQPYLCSLVDVIGSNGRDINRIVTS-SNCRNELERWQHRSLGPCDGILGSILTCQTCS 180
YN P CSLVDV+ SNG RI+TS S +ELERWQ L P DGILGSILTCQ+CS
Sbjct: 174 DYNHPPQCSLVDVLASNG----RIITSRSKSPSELERWQGHFLVPFDGILGSILTCQSCS 233
Query: 181 SQISLSFETFQNLPLSLVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDI 240
SQISLSFE+F NLPLS VL GDSTIV GCTL+DCLKQFF EK+ENY CS W
Sbjct: 234 SQISLSFESFHNLPLSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSSCWH------ 293
Query: 241 SLLWWKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCT 300
D L++ + ++ + S +L K W NK S T
Sbjct: 294 -----------DAGLKYLSSMGASKMEIEELMKCDGQDSCGCDRLFDLYKVPWSNKFSFT 353
Query: 301 LIQLTIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQL 360
L QL+IA CPKILCIHL RVS N+FGD++K++GHI FPLI+DMSPFM+ GVEI+D+E+QL
Sbjct: 354 LKQLSIAHCPKILCIHLKRVSTNMFGDVLKLQGHISFPLILDMSPFMLTGVEIKDIEKQL 413
Query: 361 GGQHSASKVKQ--IQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATI---------ES 420
G QHS++K + V+ + + ++ NG + G + + L + + +
Sbjct: 414 GKQHSSNKKPSHGLDVFRRQFDTTMLNYING---LTGEDDYPTNLASGVPFCSKQLKPSA 473
Query: 421 VSISKERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYR 480
V S SS + TR I +E+ LYR+VSVV+HFG GGGHYTVYR
Sbjct: 474 VETSSSETRSSSETWDSNLCSSSLDTRTSQIEPSETYLYRVVSVVQHFGRAGGGHYTVYR 533
Query: 481 RVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHDQWFYVSDSEVRLVSQQQVLGAR 538
RVQD + R QD+ +WF+VSDSEV LVS+++VL A
Sbjct: 534 RVQDSDA----------RLQDVS--------------TEWFHVSDSEVHLVSEEEVLAAE 554
BLAST of MS020393 vs. TAIR 10
Match:
AT4G39370.1 (ubiquitin-specific protease 27 )
HSP 1 Score: 256.5 bits (654), Expect = 4.7e-68
Identity = 196/536 (36.57%), Postives = 267/536 (49.81%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFK--LHERMSETFSLTVSLAALL 69
LVPGLQN GNNCF NVI+Q+LASC+ F+ FL+ ++ + + L E LT +L+ALL
Sbjct: 72 LVPGLQNLGNNCFLNVILQALASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALL 131
Query: 70 EELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQPYL 129
+EL + ++ VS+P KVM + +A NF+LT QQDAAEA L L+S L+EEI +P
Sbjct: 132 QELGTVGSRRSVSNPRKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQ 191
Query: 130 CS-LVDVIGSNGRDINRIVTSSNCRN--ELERWQHRSLGPCDGILGSILTCQTCSSQISL 189
S L D++ S R++ + S EL+RW GP DGILGS L C+TCSSQISL
Sbjct: 192 SSNLSDILFS--RNLRMLAPSEGLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISL 251
Query: 190 SFETFQNLPLS-LVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISLLW 249
F+ F LPLS L+ G I+SGCTL CLK+F + EK+ENY C +R WH +L +
Sbjct: 252 EFQFFHTLPLSPLLHHGGYNIMSGCTLEHCLKKFLNTEKVENYFC---YRC-WHGAALKY 311
Query: 250 WKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLIQL 309
+ + ++ KL + LH + W N S L QL
Sbjct: 312 LSVIGAAETEIE--KL-----------RSCGGEDQCDCKTSLHLQRMPWSNSYSHILKQL 371
Query: 310 TIARCPKILCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEIRDLEQQLGGQH 369
IAR PK+LCI + R S N+F + K+ GHI FPL++++S F
Sbjct: 372 IIARFPKLLCIQVQRASFNMFEEF-KLSGHIAFPLVLNLSLF------------------ 431
Query: 370 SASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSIS-KERHPSSQAN 429
T S+ ++ +ER S
Sbjct: 432 -----------------------------------------TPSSIGVNIEERIEMSSEY 491
Query: 430 SKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLKRKK 489
KP A+ +YRLV+VVEHFG G GHYTVYR V+ +S ++
Sbjct: 492 QKPE-------------ASKNHGMYRLVTVVEHFGRTGSGHYTVYRSVRVFSQEE----- 494
Query: 490 GKRRAQDLDWNFPTVSKDQDHDHD-QWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
+++D D D WF +SDSEV VS+ VLGA A LLFYE++
Sbjct: 552 ----------------EEEDCDEDLSWFSISDSEVCRVSESDVLGAEASLLFYERL 494
BLAST of MS020393 vs. TAIR 10
Match:
AT4G39370.3 (ubiquitin-specific protease 27 )
HSP 1 Score: 248.4 bits (633), Expect = 1.3e-65
Identity = 196/547 (35.83%), Postives = 267/547 (48.81%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFK--LHERMSETFSLTVSLAALL 69
LVPGLQN GNNCF NVI+Q+LASC+ F+ FL+ ++ + + L E LT +L+ALL
Sbjct: 72 LVPGLQNLGNNCFLNVILQALASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALL 131
Query: 70 EELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQPYL 129
+EL + ++ VS+P KVM + +A NF+LT QQDAAEA L L+S L+EEI +P
Sbjct: 132 QELGTVGSRRSVSNPRKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQ 191
Query: 130 CS-LVDVIGSNGRDINRIVTSSNCRN--ELERWQHRSLGPCDGILGSILTCQTCSSQISL 189
S L D++ S R++ + S EL+RW GP DGILGS L C+TCSSQISL
Sbjct: 192 SSNLSDILFS--RNLRMLAPSEGLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISL 251
Query: 190 SFETFQNLPLS-LVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNWRWQWHDISLLW 249
F+ F LPLS L+ G I+SGCTL CLK+F + EK+ENY C +R WH +L +
Sbjct: 252 EFQFFHTLPLSPLLHHGGYNIMSGCTLEHCLKKFLNTEKVENYFC---YRC-WHGAALKY 311
Query: 250 WKLWQWLDIFLQWWKLWQWHSIFLQWWKLWEWHYSISLAKLLHFAKFLWWNKPSCTLIQL 309
+ + ++ KL + LH + W N S L QL
Sbjct: 312 LSVIGAAETEIE--KL-----------RSCGGEDQCDCKTSLHLQRMPWSNSYSHILKQL 371
Query: 310 TIARCPKI-----------LCIHLNRVSVNVFGDLVKVEGHIPFPLIMDMSPFMMRGVEI 369
IAR PK+ LCI + R S N+F + K+ GHI FPL++++S F
Sbjct: 372 IIARFPKVFAYSLKLDVKLLCIQVQRASFNMFEEF-KLSGHIAFPLVLNLSLF------- 431
Query: 370 RDLEQQLGGQHSASKVKQIQVWSKICEFCVIFLNNGWMMVLGREWFRIILQATIESVSIS 429
T S+ ++
Sbjct: 432 ----------------------------------------------------TPSSIGVN 491
Query: 430 -KERHPSSQANSKPVKIPHNSATRAVNIAATESCLYRLVSVVEHFGIVGGGHYTVYRRVQ 489
+ER S KP A+ +YRLV+VVEHFG G GHYTVYR V+
Sbjct: 492 IEERIEMSSEYQKPE-------------ASKNHGMYRLVTVVEHFGRTGSGHYTVYRSVR 505
Query: 490 DYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDHD-QWFYVSDSEVRLVSQQQVLGARAM 538
+S ++ +++D D D WF +SDSEV VS+ VLGA A
Sbjct: 552 VFSQEE---------------------EEEDCDEDLSWFSISDSEVCRVSESDVLGAEAS 505
BLAST of MS020393 vs. TAIR 10
Match:
AT4G39370.2 (ubiquitin-specific protease 27 )
HSP 1 Score: 193.7 bits (491), Expect = 3.7e-49
Identity = 112/229 (48.91%), Postives = 147/229 (64.19%), Query Frame = 0
Query: 10 LVPGLQNYGNNCFFNVIMQSLASCRVFQLFLRRLVREFK--LHERMSETFSLTVSLAALL 69
LVPGLQN GNNCF NVI+Q+LASC+ F+ FL+ ++ + + L E LT +L+ALL
Sbjct: 72 LVPGLQNLGNNCFLNVILQALASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALL 131
Query: 70 EELSAIHPKKVVSSPWKVMRAMAHHAPNFSLTCQQDAAEAFLQLVSCLKEEISGYNQPYL 129
+EL + ++ VS+P KVM + +A NF+LT QQDAAEA L L+S L+EEI +P
Sbjct: 132 QELGTVGSRRSVSNPRKVMVTLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQ 191
Query: 130 CS-LVDVIGSNGRDINRIVTSSNCRN--ELERWQHRSLGPCDGILGSILTCQTCSSQISL 189
S L D++ S R++ + S EL+RW GP DGILGS L C+TCSSQISL
Sbjct: 192 SSNLSDILFS--RNLRMLAPSEGLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISL 251
Query: 190 SFETFQNLPLS-LVLDGDSTIVSGCTLVDCLKQFFDVEKIENYHCSYNW 233
F+ F LPLS L+ G I+SGCTL CLK+F + EK+ENY C W
Sbjct: 252 EFQFFHTLPLSPLLHHGGYNIMSGCTLEHCLKKFLNTEKVENYFCYRCW 298
BLAST of MS020393 vs. TAIR 10
Match:
AT2G32780.1 (ubiquitin-specific protease 1 )
HSP 1 Score: 51.6 bits (122), Expect = 2.3e-06
Identity = 32/92 (34.78%), Postives = 45/92 (48.91%), Query Frame = 0
Query: 446 LYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDH 505
+YRL +VEH G + GHY Y R G + +D D P S
Sbjct: 1012 VYRLAGLVEHLGAMSRGHYVSYIR------------GGHKERRDSDTKEPNSS------- 1071
Query: 506 DQWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
W++ SDS+VR S ++VL + A +LFYE+I
Sbjct: 1072 -IWYHASDSQVRPASLEEVLRSEAYILFYERI 1083
BLAST of MS020393 vs. TAIR 10
Match:
AT1G04860.1 (ubiquitin-specific protease 2 )
HSP 1 Score: 50.4 bits (119), Expect = 5.1e-06
Identity = 33/92 (35.87%), Postives = 45/92 (48.91%), Query Frame = 0
Query: 446 LYRLVSVVEHFGIVGGGHYTVYRRVQDYSSQKLKRKKGKRRAQDLDWNFPTVSKDQDHDH 505
+YRL +VEH G + GGHY Y R G +R K+ D
Sbjct: 890 VYRLAGLVEHSGTMRGGHYVAYVR-------------GGQRV-----------KETDSSS 949
Query: 506 DQWFYVSDSEVRLVSQQQVLGARAMLLFYEKI 538
W+ VSD+ VR VS ++VL + A +LFYE+I
Sbjct: 950 TAWYNVSDAYVRQVSLEKVLHSEAYILFYERI 957
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022154188.1 | 1.9e-132 | 53.10 | ubiquitin carboxyl-terminal hydrolase 27 isoform X1 [Momordica charantia] | [more] |
XP_004148458.1 | 1.1e-127 | 52.17 | ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus] | [more] |
XP_008457921.1 | 5.4e-127 | 52.09 | PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo] >KAA0045908.1... | [more] |
XP_023513383.1 | 5.4e-127 | 52.01 | ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita pepo subsp. pepo] | [more] |
XP_022928102.1 | 1.6e-126 | 51.00 | ubiquitin carboxyl-terminal hydrolase 27 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9FPS0 | 6.6e-67 | 36.57 | Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana OX=3702 GN=UBP2... | [more] |
A4QNN3 | 3.5e-20 | 23.51 | Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis OX=8364 GN=usp30 ... | [more] |
Q3UN04 | 2.5e-18 | 24.86 | Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus OX=10090 GN=Usp30 PE=1 ... | [more] |
D3ZPG5 | 3.8e-14 | 24.68 | Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus OX=10116 GN=Usp30 ... | [more] |
Q70CQ3 | 1.1e-13 | 23.61 | Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens OX=9606 GN=USP30 PE=1 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DJL7 | 9.3e-133 | 53.10 | Ubiquitin carboxyl-terminal hydrolase OS=Momordica charantia OX=3673 GN=LOC11102... | [more] |
A0A0A0LJP9 | 5.3e-128 | 52.17 | USP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G298270 PE=3 SV... | [more] |
A0A5A7TSR6 | 2.6e-127 | 52.09 | Ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo var. makuwa OX=1194695 ... | [more] |
A0A1S3C667 | 2.6e-127 | 52.09 | ubiquitin carboxyl-terminal hydrolase 27 OS=Cucumis melo OX=3656 GN=LOC103497488... | [more] |
A0A6J1EIY6 | 7.6e-127 | 51.00 | ubiquitin carboxyl-terminal hydrolase 27 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |