MS020024 (gene) Bitter gourd (TR) v1

Overview
NameMS020024
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDUF1645 domain-containing protein
Locationscaffold22: 1278547 .. 1279473 (+)
RNA-Seq ExpressionMS020024
SyntenyMS020024
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCAAGGTGGTGAAGGACCGCTACTTCTTTCCCTCTCTCCAAGCTTTAGCAGCTACTCCTCCGGCAGATTTGCTGAAATTGCCGCCCGTGTCGTTGAGGAATTCCGGAAAGAATCCGATTCTGATCCCGATATTTTCGACTGGAAAACTGATGCTTCTTCGCTCCAACACGGAGGCGAACCGGAAGAATTCAACCTAGCAGCTCACCAGGATGGTAATGGTGGAACTGAGACTGTTGTTGGTGAAGTCGGAGACAACGATGAGTTCGAATTCGCTGTCGTTCCGAGGGAGCCGCAGGCGATGACGACCTCGGCTGAGGATATCTTCTACAACGGCCAGATTAGACCGATTTATCCGGTTTTCAACACGAATCTGTTGTTGAACGGTTCTCTTACAGATAAGAGTAAAAACGTTGGCGGCAACGAAGACTTGAAGAAATCCAAGGCGACGAGAGTTCATCGTCCGTCGCTGGGGAAACTGATGAGCGAAGAGCGTGAGTCGAATTCTTGCTCGTCGTCTGAGGCCGACGAGCTTGACGGAGTTATTCCTGGAACGTACTGCGTCTGGTCGCCGAAGGAATCGCTCGAGAGATGCAAAAAGAGCAATTCGATGGGCTCGTCGAAGAGATGGAAGCTCCGAGACCTCTTATACAGAAGCAATAGCGACGGAAAGGACACGTTCGTGTTTTTAGCACCAAACAAGAGAGTTGAGAAAGTCGCCGAGGTCTCAAAGGGAAAAGGTAGTAGCGGTACCGGTGACGAAAAGACCGCCGGAAAAGTAAAGCCGAGCAGAGTCGCCGCTGCAACGGCTGCTAATGGCGCGACGGAGGGAGCGGATACGAGGAGTCAATCGGTTCCGTATACACAGGAAACGGTGGATTTATTCGCCGAGGTCAATGGTATGAACAAAAACTCACATCTATTT

mRNA sequence

ATGATGCAAGGTGGTGAAGGACCGCTACTTCTTTCCCTCTCTCCAAGCTTTAGCAGCTACTCCTCCGGCAGATTTGCTGAAATTGCCGCCCGTGTCGTTGAGGAATTCCGGAAAGAATCCGATTCTGATCCCGATATTTTCGACTGGAAAACTGATGCTTCTTCGCTCCAACACGGAGGCGAACCGGAAGAATTCAACCTAGCAGCTCACCAGGATGGTAATGGTGGAACTGAGACTGTTGTTGGTGAAGTCGGAGACAACGATGAGTTCGAATTCGCTGTCGTTCCGAGGGAGCCGCAGGCGATGACGACCTCGGCTGAGGATATCTTCTACAACGGCCAGATTAGACCGATTTATCCGGTTTTCAACACGAATCTGTTGTTGAACGGTTCTCTTACAGATAAGAGTAAAAACGTTGGCGGCAACGAAGACTTGAAGAAATCCAAGGCGACGAGAGTTCATCGTCCGTCGCTGGGGAAACTGATGAGCGAAGAGCGTGAGTCGAATTCTTGCTCGTCGTCTGAGGCCGACGAGCTTGACGGAGTTATTCCTGGAACGTACTGCGTCTGGTCGCCGAAGGAATCGCTCGAGAGATGCAAAAAGAGCAATTCGATGGGCTCGTCGAAGAGATGGAAGCTCCGAGACCTCTTATACAGAAGCAATAGCGACGGAAAGGACACGTTCGTGTTTTTAGCACCAAACAAGAGAGTTGAGAAAGTCGCCGAGGTCTCAAAGGGAAAAGGTAGTAGCGGTACCGGTGACGAAAAGACCGCCGGAAAAGTAAAGCCGAGCAGAGTCGCCGCTGCAACGGCTGCTAATGGCGCGACGGAGGGAGCGGATACGAGGAGTCAATCGGTTCCGTATACACAGGAAACGGTGGATTTATTCGCCGAGGTCAATGGTATGAACAAAAACTCACATCTATTT

Coding sequence (CDS)

ATGATGCAAGGTGGTGAAGGACCGCTACTTCTTTCCCTCTCTCCAAGCTTTAGCAGCTACTCCTCCGGCAGATTTGCTGAAATTGCCGCCCGTGTCGTTGAGGAATTCCGGAAAGAATCCGATTCTGATCCCGATATTTTCGACTGGAAAACTGATGCTTCTTCGCTCCAACACGGAGGCGAACCGGAAGAATTCAACCTAGCAGCTCACCAGGATGGTAATGGTGGAACTGAGACTGTTGTTGGTGAAGTCGGAGACAACGATGAGTTCGAATTCGCTGTCGTTCCGAGGGAGCCGCAGGCGATGACGACCTCGGCTGAGGATATCTTCTACAACGGCCAGATTAGACCGATTTATCCGGTTTTCAACACGAATCTGTTGTTGAACGGTTCTCTTACAGATAAGAGTAAAAACGTTGGCGGCAACGAAGACTTGAAGAAATCCAAGGCGACGAGAGTTCATCGTCCGTCGCTGGGGAAACTGATGAGCGAAGAGCGTGAGTCGAATTCTTGCTCGTCGTCTGAGGCCGACGAGCTTGACGGAGTTATTCCTGGAACGTACTGCGTCTGGTCGCCGAAGGAATCGCTCGAGAGATGCAAAAAGAGCAATTCGATGGGCTCGTCGAAGAGATGGAAGCTCCGAGACCTCTTATACAGAAGCAATAGCGACGGAAAGGACACGTTCGTGTTTTTAGCACCAAACAAGAGAGTTGAGAAAGTCGCCGAGGTCTCAAAGGGAAAAGGTAGTAGCGGTACCGGTGACGAAAAGACCGCCGGAAAAGTAAAGCCGAGCAGAGTCGCCGCTGCAACGGCTGCTAATGGCGCGACGGAGGGAGCGGATACGAGGAGTCAATCGGTTCCGTATACACAGGAAACGGTGGATTTATTCGCCGAGGTCAATGGTATGAACAAAAACTCACATCTATTT

Protein sequence

MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGGEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVNGMNKNSHLF
Homology
BLAST of MS020024 vs. NCBI nr
Match: XP_022137020.1 (uncharacterized protein LOC111008584 [Momordica charantia])

HSP 1 Score: 606.3 bits (1562), Expect = 1.5e-169
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG 60
           MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG
Sbjct: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG 60

Query: 61  EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP 120
           EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP
Sbjct: 61  EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP 120

Query: 121 VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD 180
           VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD
Sbjct: 121 VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD 180

Query: 181 GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV 240
           GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV
Sbjct: 181 GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV 240

Query: 241 AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN 300
           AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN
Sbjct: 241 AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN 300

Query: 301 GMNKNSHLF 310
           GMNKNSHLF
Sbjct: 301 GMNKNSHLF 309

BLAST of MS020024 vs. NCBI nr
Match: KAG7019821.1 (hypothetical protein SDJN02_18784, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 449.5 bits (1155), Expect = 2.3e-122
Identity = 246/329 (74.77%), Postives = 264/329 (80.24%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQGGEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK D ASS QHG
Sbjct: 1   MMQGGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAASSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK++NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSIIDKNRNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEK AGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKAAGKVKSSRVAAAAAATAAIVTPAASDEGQHVKNQSKEAD 300

Query: 301 TRSQSVPYTQETVDLFAEVNGMNKNSHLF 310
            + QS+ Y QET DL   VNGM KNSH F
Sbjct: 301 RKRQSIAYGQETADLLKNVNGMTKNSHQF 323

BLAST of MS020024 vs. NCBI nr
Match: XP_038893499.1 (uncharacterized protein LOC120082410 [Benincasa hispida])

HSP 1 Score: 441.0 bits (1133), Expect = 8.2e-120
Identity = 243/332 (73.19%), Postives = 267/332 (80.42%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQ GEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESD DPDIF+WK D ASSLQHG
Sbjct: 1   MMQSGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDPDPDIFNWKPDVASSLQHG 60

Query: 61  GEPEEFNLAAH-QDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPI 120
            EPEEFN A   QDGNG TETV  E+ D DEFEFAVVPREPQ +T SAEDIFYNGQIRPI
Sbjct: 61  PEPEEFNQAPQVQDGNGETETVGDEIAD-DEFEFAVVPREPQEVTISAEDIFYNGQIRPI 120

Query: 121 YPVFNTNLLLNGSLTDKSKNVGGNED-----LKKSKATRVHRPSLGKLMSEERESNSCSS 180
           YPVFNTNLLL+G++ DK+ +   +ED     LKKSK  RVHRPSLGKLMSEERE+NSCSS
Sbjct: 121 YPVFNTNLLLSGAIVDKNNSNVDDEDDDGKLLKKSKPKRVHRPSLGKLMSEERETNSCSS 180

Query: 181 SEADELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAP 240
           SEA+ELDGV+PGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGK+TFVFL+ 
Sbjct: 181 SEAEELDGVLPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKETFVFLSS 240

Query: 241 NKRVEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA------------------ 300
           +KRVEK AE+SK KG++GTGDEK +GKVK SRVAAA AA  A                  
Sbjct: 241 SKRVEKFAEISKEKGTTGTGDEKISGKVKSSRVAAAAAAAAAATATNVSPPAADEGEQVK 300

Query: 301 --TEGADTRSQSVPYTQETVDLFAEVNGMNKN 306
             ++ AD ++QS+P  QE  DLF  VNGMNKN
Sbjct: 301 NSSKEADRKNQSIPCRQEMTDLFNNVNGMNKN 331

BLAST of MS020024 vs. NCBI nr
Match: XP_023519077.1 (uncharacterized protein LOC111782544 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437.6 bits (1124), Expect = 9.1e-119
Identity = 241/322 (74.84%), Postives = 259/322 (80.43%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQGGEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK D ASS QHG
Sbjct: 1   MMQGGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAASSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK++NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSIIDKNRNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA-----------------TEGA 300
           VEK+AEVSK K      DEKTAGKVK SRVAAA AA  A                 ++ A
Sbjct: 241 VEKIAEVSKEK-----IDEKTAGKVKSSRVAAAAAAATAANVTPAASDEGQHVKNQSKDA 300

Query: 301 DTRSQSVPYTQETVDLFAEVNG 302
           D + QS+ Y QET DL   VNG
Sbjct: 301 DRKRQSIAYGQETADLLKNVNG 316

BLAST of MS020024 vs. NCBI nr
Match: KAG6584223.1 (hypothetical protein SDJN03_20155, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.2e-118
Identity = 240/321 (74.77%), Postives = 258/321 (80.37%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQGGEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK D ASS QHG
Sbjct: 1   MMQGGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAASSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK++NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSIIDKNRNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEK AGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKAAGKVKSSRVAAAAAATAAIVTPAASDEGQHVKNQSKEAD 300

Query: 301 TRSQSVPYTQETVDLFAEVNG 302
            + QS+ Y QET DL   VNG
Sbjct: 301 RKRQSIAYGQETADLLKNVNG 315

BLAST of MS020024 vs. ExPASy TrEMBL
Match: A0A6J1C5I3 (uncharacterized protein LOC111008584 OS=Momordica charantia OX=3673 GN=LOC111008584 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.2e-170
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG 60
           MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG
Sbjct: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGG 60

Query: 61  EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP 120
           EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP
Sbjct: 61  EPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYP 120

Query: 121 VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD 180
           VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD
Sbjct: 121 VFNTNLLLNGSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELD 180

Query: 181 GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV 240
           GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV
Sbjct: 181 GVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKV 240

Query: 241 AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN 300
           AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN
Sbjct: 241 AEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAEVN 300

Query: 301 GMNKNSHLF 310
           GMNKNSHLF
Sbjct: 301 GMNKNSHLF 309

BLAST of MS020024 vs. ExPASy TrEMBL
Match: A0A6J1EE75 (uncharacterized protein LOC111431736 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431736 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 7.5e-119
Identity = 240/321 (74.77%), Postives = 258/321 (80.37%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQGGEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK D ASS QHG
Sbjct: 1   MMQGGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAASSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK++NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSILDKNRNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEKTAGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKTAGKVKSSRVAAAAAATAANITPAASDEGQHVKNQSKEAD 300

Query: 301 TRSQSVPYTQETVDLFAEVNG 302
            + QS+ Y QE  DL   VNG
Sbjct: 301 RKRQSIAYGQERADLLKNVNG 315

BLAST of MS020024 vs. ExPASy TrEMBL
Match: A0A6J1KFR5 (uncharacterized protein LOC111495389 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495389 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.3e-118
Identity = 240/321 (74.77%), Postives = 257/321 (80.06%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDA-SSLQHG 60
           MMQG EG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK DA SS QHG
Sbjct: 1   MMQGSEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAESSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK+ NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSIIDKNSNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEKTAGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKTAGKVKSSRVAAAAAATAANVTPAASDEGQHVKNQSKEAD 300

Query: 301 TRSQSVPYTQETVDLFAEVNG 302
            + QS+ Y QET DL   VNG
Sbjct: 301 RKRQSIAYGQETADLLKNVNG 315

BLAST of MS020024 vs. ExPASy TrEMBL
Match: A0A6J1E8J1 (uncharacterized protein LOC111431736 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431736 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.7e-118
Identity = 239/320 (74.69%), Postives = 257/320 (80.31%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTD-ASSLQHG 60
           MMQGGEG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK D ASS QHG
Sbjct: 1   MMQGGEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAASSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK++NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSILDKNRNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEKTAGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKTAGKVKSSRVAAAAAATAANITPAASDEGQHVKNQSKEAD 300

BLAST of MS020024 vs. ExPASy TrEMBL
Match: A0A6J1KPR3 (uncharacterized protein LOC111495389 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111495389 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 6.4e-118
Identity = 239/320 (74.69%), Postives = 256/320 (80.00%), Query Frame = 0

Query: 1   MMQGGEGPLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDA-SSLQHG 60
           MMQG EG LLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSD DIFDWK DA SS QHG
Sbjct: 1   MMQGSEGSLLLSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDTDIFDWKNDAESSFQHG 60

Query: 61  GEPEEFNLAAHQDGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIY 120
            EPEEFNLA   DGNGGTE+VV E+GD DEFEF+V P EPQA+T SAEDIFYNGQIRPIY
Sbjct: 61  TEPEEFNLAPIHDGNGGTESVVDEIGD-DEFEFSVAPTEPQAVTISAEDIFYNGQIRPIY 120

Query: 121 PVFNTNLLLNGSLTDKSKNVGGNED---LKKSKATRVHRPSLGKLMSEERESNSCSSSEA 180
           PV NTNLLL+ S+ DK+ NV G +D   LKK K  RVHRPSLGKLMSEERE+NSCSSSEA
Sbjct: 121 PVINTNLLLSSSIIDKNSNVDGEDDDGNLKKPKPKRVHRPSLGKLMSEERETNSCSSSEA 180

Query: 181 DELDGVIPGTYCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKR 240
           DELDGV+PGT+CVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL P+KR
Sbjct: 181 DELDGVLPGTFCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLTPSKR 240

Query: 241 VEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGA----------------TEGAD 300
           VEK+AEVSK K      DEKTAGKVK SRVAAA AA  A                ++ AD
Sbjct: 241 VEKIAEVSKEK-----IDEKTAGKVKSSRVAAAAAATAANVTPAASDEGQHVKNQSKEAD 300

BLAST of MS020024 vs. TAIR 10
Match: AT5G62770.1 (Protein of unknown function (DUF1645) )

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-26
Identity = 110/320 (34.38%), Postives = 149/320 (46.56%), Query Frame = 0

Query: 12  SLSPSFSSYSSG-RFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGGEPEEFNLAAH 71
           S SPSF S+SS    A IAARVVEEFR    +  D                      + H
Sbjct: 11  SNSPSFGSFSSAVDLAAIAARVVEEFRDHDQTQSD---------------------SSPH 70

Query: 72  QDGNGGTETVVGEVGDND-EFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLN 131
           +D             DND +F F            +A++IF NGQIRP+ P +  N  + 
Sbjct: 71  RD------------DDNDSDFAFDCPSNTCSQPLATADEIFCNGQIRPLNP-YGGNAPVE 130

Query: 132 GSLTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADE-LDGVIPGTYC 191
              T K   +            R  RP+L KLMSE+R+  S SSSEA+E L GV P TYC
Sbjct: 131 SQPTSKITTL----------PPRRRRPALRKLMSEDRDPASNSSSEAEEDLTGVPPETYC 190

Query: 192 VWSPKES------LERCK--------KSNSMGSSKRWKLRDLLY-RSNSDGKDTFVFLAP 251
           VW PK+S      L+R          KS+S G SKRWKLR+LLY RS+S+G D  VF AP
Sbjct: 191 VWKPKQSNSGDDDLQRLSSSPSHSKIKSHSAGFSKRWKLRNLLYVRSSSEGNDKLVFPAP 250

Query: 252 NKRVEKVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGAD----TRSQSVPYT 310
            K+ ++            T  ++   +  PS+V      +G  EG +     R   VPY 
Sbjct: 251 VKKNDE------------TVSDQREEEEPPSKV------DGEEEGREREETKRQTYVPYR 268

BLAST of MS020024 vs. TAIR 10
Match: AT1G23710.1 (Protein of unknown function (DUF1645) )

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-22
Identity = 86/251 (34.26%), Postives = 117/251 (46.61%), Query Frame = 0

Query: 86  DNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLN-GSLTDKSKNVGGNED 145
           + +EF FA V  E   +T  A++ F +GQIRP++P+FN +LL    +  DK+ NV     
Sbjct: 61  EEEEFSFACVNGEGSPIT--ADEAFEDGQIRPVFPLFNRDLLFEYENEDDKNDNV----- 120

Query: 146 LKKSKATRVHRPSLGKLMSEERESNSCSSSEADELDGVIPGTYCVWS----PKESLERCK 205
                 T  +RP L KL  E+R  N     E +  +    G YC W+     + S E C+
Sbjct: 121 ----SVTDENRPRLRKLFVEDRNGNG-DGEETEGSEKEPLGPYCSWTGGTVAEASPETCR 180

Query: 206 KSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEKVAEVSKGKGSSGTGD------ 265
           KSNS G SK W+ RDL+ RSNSDG+D FVFL  N   +K    S    SS   +      
Sbjct: 181 KSNSTGFSKLWRFRDLVLRSNSDGRDAFVFL--NNSNDKTRTRSSSSSSSTAAEENDKKV 240

Query: 266 --EKTAGKVKPSRVAAATAANGATEGADTR--------------SQSVPYTQETVDLFAE 310
             EK  GK K S  +        T+ A  +                 +PY Q  V  F  
Sbjct: 241 ITEKKKGKEKTSTSSETKKKTTTTKSAHEKLYMRNRAMKEEVKHRSYLPYKQ--VGFFTN 295

BLAST of MS020024 vs. TAIR 10
Match: AT1G70420.1 (Protein of unknown function (DUF1645) )

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-19
Identity = 79/238 (33.19%), Postives = 110/238 (46.22%), Query Frame = 0

Query: 85  GDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLNGSLTDKSKNVGGNED 144
           G  ++F FA V  +   +T  A++ F +GQIRP+YP+FN N+  +               
Sbjct: 61  GREEDFSFASVNADNSPIT--ADEAFEDGQIRPVYPLFNRNIFFD--------------- 120

Query: 145 LKKSKATRVHRPSLGKLMSEERESNSCSSSEADELDGVIPGTYCVWS----PKESLERCK 204
                  +  R  L KL  E    ++ +  E +E D V  G YC W+     + S E C+
Sbjct: 121 ---DPEEKTLRSPLKKLFVE----STTTEEEEEESDTV--GPYCSWTNRTVEQASPETCR 180

Query: 205 KSNSMGSSKRWKLRDLLYRSNSDGKDTFVFL-----APNKRVEKVAEVSKGKGSSGTGDE 264
           KSNS G SK W+ RDL+ RSNSDGKD FVFL     + +      A +S    SS  G E
Sbjct: 181 KSNSTGFSKLWRFRDLVLRSNSDGKDAFVFLSNGSSSTSSTSSTAARLSGVVKSSEKGKE 240

Query: 265 KTAGKVKPSRVAAATA------ANGATEGADTRSQSVPYTQETVDLFAEVNGMNKNSH 308
           KT    K  ++   +A       N A      R   +PY  + V  F  VNG+ +N H
Sbjct: 241 KTKTDKKKDKMRTKSAHEKLYMRNRAMREEGKRRSYLPY--KHVGFFTNVNGLTRNVH 270

BLAST of MS020024 vs. TAIR 10
Match: AT3G27880.1 (Protein of unknown function (DUF1645) )

HSP 1 Score: 72.0 bits (175), Expect = 9.4e-13
Identity = 87/306 (28.43%), Postives = 120/306 (39.22%), Query Frame = 0

Query: 12  SLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGGEPEEFNLAAHQ 71
           S SPSFS+YS  R  EIA RV   ++++SD++   F++ T A+S                
Sbjct: 9   SFSPSFSTYSGDRLVEIAERVC--YKEKSDAE---FEFSTAATS---------------S 68

Query: 72  DGNGGTETVVGEVGDNDEFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLNGS 131
            G+GG                                         ++PVFN NL+    
Sbjct: 69  SGDGGL----------------------------------------VFPVFNKNLISGDV 128

Query: 132 LTDKSKNVGGNEDLKKSKATRVHRPSLGKLMSEERESNSCSSSEADELDGVIPGTYCVWS 191
             +K   V   +DL   +      P       +   S+S    E DE D +    YC W+
Sbjct: 129 SPEK---VIPLKDLFLRERNDQQPP------QQTYSSSSDEEEEDDEFDSIPSEIYCPWT 188

Query: 192 PKESLE------RCKKSNSMGSS-------KRWKLRDLLYRSNSDGKDTFVFLAPNKRVE 251
           P  S         C+KS S GSS       KRW+LRD L RS SDGK +  FL P  R +
Sbjct: 189 PARSTADMSPSGGCRKSKSTGSSSTSTWSTKRWRLRDFLKRSKSDGKQSLKFLNPTNR-D 237

Query: 252 KVAEVSKGKGSSGTGDEKTAGKVKPSRVAAATAANGATEGADTRSQSVPYTQETVDLFAE 305
              E SK K       +K +  V  S        N A +  D R   +PY Q+ V LF+ 
Sbjct: 249 DDDESSKSK-------KKVSVSVTVSAHEKFYLRNKAIKEEDKRKSYLPYKQDLVGLFSN 237

BLAST of MS020024 vs. TAIR 10
Match: AT5G14730.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1645 (InterPro:IPR012442); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01513.1); Has 85 Blast hits to 83 proteins in 14 species: Archae - 0; Bacteria - 9; Metazoa - 0; Fungi - 0; Plants - 76; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 50.8 bits (120), Expect = 2.2e-06
Identity = 80/303 (26.40%), Postives = 117/303 (38.61%), Query Frame = 0

Query: 11  LSLSPSFSSYSSGRFAEIAARVVEEFRKESDSDPDIFDWKTDASSLQHGGEPEEFNLAAH 70
           +S SPSFS Y+SG   E A RV+    +ES+S    +  K D                  
Sbjct: 11  VSFSPSFSFYASGDLVETAVRVI----RESES---YYSVKVD------------------ 70

Query: 71  QDGNGGTETVVGEVGDND-EFEFAVVPREPQAMTTSAEDIFYNGQIRPIYPVFNTNLLLN 130
                         G+ND EFEF  +P          E +F+          F+T     
Sbjct: 71  --------------GENDKEFEFETLP-------LGDESLFH----------FST----- 130

Query: 131 GSLTDKSKNVGGNEDLKKSKAT---------RVHRPSLGKLMSEERESNSCSSSEADELD 190
             +T   K    ++D+   + T          V  PSL         S S SSS++D+ +
Sbjct: 131 --MTTTFKRSSDDDDVADDRVTSKNLFYGGWSVDPPSL------TSPSESLSSSDSDDSE 190

Query: 191 GVIPGT-YCVWSPKESLERCKKSNSMGSSKRWKLRDLLYRSNSDGKDTFVFLAPNKRVEK 250
            + P   YC WSP  S  R     S GSS+R ++++ L RS+SDG  + V        + 
Sbjct: 191 NISPSKFYCFWSPIRSPARDDSLKSKGSSRRCRIKEFLRRSHSDGAVSTVSATKRCSFKD 239

Query: 251 VAEVSKGKGSSGTGDEKTAGK----VKPSRVAAATAANGATEGADTRSQS-VPYTQETVD 298
           +   S   G        T+G     VK     AA   +      D R +S +PY Q+ + 
Sbjct: 251 LLRRSHSDGGGEGSSISTSGNGSLVVKGRNKTAAYKPD-----VDKRRKSYLPYRQDLIG 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137020.11.5e-169100.00uncharacterized protein LOC111008584 [Momordica charantia][more]
KAG7019821.12.3e-12274.77hypothetical protein SDJN02_18784, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_038893499.18.2e-12073.19uncharacterized protein LOC120082410 [Benincasa hispida][more]
XP_023519077.19.1e-11974.84uncharacterized protein LOC111782544 isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6584223.11.2e-11874.77hypothetical protein SDJN03_20155, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C5I37.2e-170100.00uncharacterized protein LOC111008584 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1EE757.5e-11974.77uncharacterized protein LOC111431736 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KFR51.3e-11874.77uncharacterized protein LOC111495389 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E8J13.7e-11874.69uncharacterized protein LOC111431736 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KPR36.4e-11874.69uncharacterized protein LOC111495389 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G62770.13.3e-2634.38Protein of unknown function (DUF1645) [more]
AT1G23710.12.2e-2234.26Protein of unknown function (DUF1645) [more]
AT1G70420.11.8e-1933.19Protein of unknown function (DUF1645) [more]
AT3G27880.19.4e-1328.43Protein of unknown function (DUF1645) [more]
AT5G14730.12.2e-0626.40unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1645... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012442Protein of unknown function DUF1645, plantPFAMPF07816DUF1645coord: 106..268
e-value: 1.6E-24
score: 87.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..287
NoneNo IPR availablePANTHERPTHR33095FAMILY NOT NAMEDcoord: 10..304
NoneNo IPR availablePANTHERPTHR33095:SF23DUF1645 FAMILY PROTEINcoord: 10..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020024.1MS020024.1mRNA