Homology
BLAST of MS019945 vs. NCBI nr
Match:
XP_022137541.1 (4-coumarate--CoA ligase-like 9 [Momordica charantia] >XP_022137542.1 4-coumarate--CoA ligase-like 9 [Momordica charantia] >XP_022137543.1 4-coumarate--CoA ligase-like 9 [Momordica charantia])
HSP 1 Score: 1066.2 bits (2756), Expect = 9.5e-308
Identity = 553/565 (97.88%), Postives = 553/565 (97.88%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPI QPLSITDHALSLVQSSPPP
Sbjct: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPIPQPLSITDHALSLVQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL
Sbjct: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT 180
LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT
Sbjct: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT 180
Query: 181 ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR 240
ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR
Sbjct: 181 ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR 240
Query: 241 TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA 300
TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA
Sbjct: 241 TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA 300
Query: 301 LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG 360
LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG
Sbjct: 301 LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG 360
Query: 361 PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL 420
PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL
Sbjct: 361 PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL 420
Query: 421 HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY 480
HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY
Sbjct: 421 HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY 480
Query: 481 PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINAI 540
PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAK QVAPYKKIRRVSFINAI
Sbjct: 481 PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAK-----------QVAPYKKIRRVSFINAI 540
Query: 541 PKSPAGKILRRELVSHALSQGSNKL 566
PKSPAGKILRRELVSHALSQGSNKL
Sbjct: 541 PKSPAGKILRRELVSHALSQGSNKL 554
BLAST of MS019945 vs. NCBI nr
Match:
XP_022923628.1 (4-coumarate--CoA ligase-like 9 [Cucurbita moschata])
HSP 1 Score: 890.2 bits (2299), Expect = 9.4e-255
Identity = 460/569 (80.84%), Postives = 505/569 (88.75%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNP+LN AAHFVD RSGFCPQTKIFHSLRPPL +PP+SQPLS+T+HALSL+QSSPPP
Sbjct: 1 MADRNPSLN-AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
+N LVD +SGVH+SYA+ LR+IR L++NLKALTSLS+GQVAFILAPTSL+VPVLYFAL
Sbjct: 61 SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV ISPANP GS+SEI+HQ++LSKPVIAFATSSTA+KLP+ +LG +LIDSP FLSMM
Sbjct: 121 LSLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVEQGE 240
TE NRSDG+ADVK++Q+DSAAILYSSGTTGRVKGV LSHRNLI NS+ L S V GE
Sbjct: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
EPR VSL LLPLFHVFGF M++R ISRG+TLVLMQ+FDF ML AVEK+ VTYIPVSPP
Sbjct: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P VEIIQGYGLTESTA AA
Sbjct: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
R++GPEE SNT SVGRLSE+MEA IVDP SGEALPPGHRGELWLRGPGIMKGYV D KAT
Sbjct: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETLHP+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDE+AGEIPMAYVVRKPGSNI+E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HA S GSNKL
Sbjct: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 557
BLAST of MS019945 vs. NCBI nr
Match:
XP_038894373.1 (4-coumarate--CoA ligase-like 9 [Benincasa hispida])
HSP 1 Score: 888.6 bits (2295), Expect = 2.7e-254
Identity = 458/569 (80.49%), Postives = 505/569 (88.75%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRN N N+AAHFVD RSGFCPQT+IFHSLRPPLSLPP+SQPLS+ +HALSL+QSSPPP
Sbjct: 1 MADRNLNFNAAAHFVDLRSGFCPQTRIFHSLRPPLSLPPLSQPLSVAEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
AN+T L+D +SG+H+SYA+ LR+IR L++NLKALTSLS GQVAFILAPTSLQVPVLYFAL
Sbjct: 61 ANSTLLIDSNSGLHLSYAIFLRQIRTLASNLKALTSLSHGQVAFILAPTSLQVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV +SPANPT S+SEI+HQ++LSKPVIAFATSSTA+KLP + G +LIDSP+FLSMM
Sbjct: 121 LSLGVILSPANPTSSKSEISHQIRLSKPVIAFATSSTASKLPALRFGTVLIDSPQFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAAN---STLASLVEQGE 240
TE NR D +AD+KI+Q+DSAAILYSSGTTGRVKGV LSHRNLIA N S L S + +GE
Sbjct: 181 TEINRPDTLADIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPSALQSEINEGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
+EP VSL LLPLFHVFGF M++R ISRG+TLVLMQRFDFEGML AVEK+ VTYIPVSPP
Sbjct: 241 MEPHPVSLCLLPLFHVFGFVMLLREISRGETLVLMQRFDFEGMLNAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P+VEIIQGYGLTESTA AA
Sbjct: 301 LVVAMAKSELVAKYDLSSLQMLGCGGAPLGKEVIEKFHQKMPSVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
RTLGPEE SNT SVGRLS S+EAKIVDPASGEALPP H+GELWLRGP IMKGYV DDKAT
Sbjct: 361 RTLGPEESSNTKSVGRLSGSIEAKIVDPASGEALPPDHKGELWLRGPVIMKGYVGDDKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETL P+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 NETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDEEAGEIPMAY+VRKPGSNI+E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYIVRKPGSNITEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AI KSPAGKILRREL HALS GSNKL
Sbjct: 541 IDAITKSPAGKILRRELAKHALSHGSNKL 558
BLAST of MS019945 vs. NCBI nr
Match:
KAG6584140.1 (4-coumarate--CoA ligase-like 9, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 887.9 bits (2293), Expect = 4.7e-254
Identity = 459/569 (80.67%), Postives = 504/569 (88.58%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNP+LN AAHFVD RSGFCPQTKIFHSLRPPL +PP+SQPLS+T+HALSL+QSSPPP
Sbjct: 1 MADRNPSLN-AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
+N LVD +SGVH+SYA+ LR+IR L++NLKALTSLS+GQVAFILAPTSL+VPVLYFAL
Sbjct: 61 SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV ISPANP GS+SEI+HQ++LS+PVIAFATSSTA+KLP+ LG +LIDSP FLSMM
Sbjct: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVEQGE 240
TE NRSDG+ADVK++Q+DSAAILYSSGTTGRVKGV LSHRNLI NS+ L S V GE
Sbjct: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
EPR VSL LLPLFHVFGF M++R ISRG+TLVLMQ+FDF ML AVEK+ VTYIPVSPP
Sbjct: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P VEIIQGYGLTESTA AA
Sbjct: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
R++GPEE SNT SVGRLSE+MEA IVDP SGEALPPGHRGELWLRGPGIMKGYV D KAT
Sbjct: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETLHP+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDE+AGEIPMAYVVRKPGSNI+E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HA S GSNKL
Sbjct: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 557
BLAST of MS019945 vs. NCBI nr
Match:
KAG7019731.1 (4-coumarate--CoA ligase-like 9 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 887.9 bits (2293), Expect = 4.7e-254
Identity = 459/569 (80.67%), Postives = 504/569 (88.58%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNP+LN AAHFVD RSGFCPQTKIFHSLRPPL +PP+SQPLS+T+HALSL+QSSPPP
Sbjct: 1 MADRNPSLN-AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
+N LVD +SGVH+SYA+ LR+IR L++NLKALTSLS+GQVAFILAPTSL+VPVLYFAL
Sbjct: 61 SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV ISPANP GS+SEI+HQ++LS+PVIAFATSSTA+KLP+ LG +LIDSP FLSMM
Sbjct: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVEQGE 240
TE NRSDG+ADVK++Q+DSAAILYSSGTTGRVKGV LSHRNLI NS+ L S V GE
Sbjct: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
EPR VSL LLPLFHVFGF M++R ISRG+TLVLMQ+FDF ML AVEK+ VTYIPVSPP
Sbjct: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P VEIIQGYGLTESTA AA
Sbjct: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
R++GPEE SNT SVGRLSE+MEA IVDP SGEALPPGHRGELWLRGPGIMKGYV D KAT
Sbjct: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETLHP+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDE+AGEIPMAYVVRKPGSNI+E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HA S GSNKL
Sbjct: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 557
BLAST of MS019945 vs. ExPASy Swiss-Prot
Match:
Q84P23 (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)
HSP 1 Score: 665.6 bits (1716), Expect = 4.9e-190
Identity = 348/566 (61.48%), Postives = 431/566 (76.15%), Query Frame = 0
Query: 12 AHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANT-------T 71
+ +D SGF +T I+HSLRP LSLPPI QPLS + ALSL+ S PPA T
Sbjct: 8 SRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALT 67
Query: 72 ALVDFDSGVHVSYALLLREIRNLSANLK-ALTSLSSGQVAFILAPTSLQVPVLYFALLSI 131
LV+ SG +++Y LLR +R+L+ +L+ SL+S VAFIL+P+SL +PVLY AL+SI
Sbjct: 68 YLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSI 127
Query: 132 GVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRK---LGKILIDSPEFLSMMT 191
GV +SPANP GSESE++HQV++S+PVIAFATS T KL LG +L+DS EFLS +
Sbjct: 128 GVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLN 187
Query: 192 ESNRSD-GVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEP 251
S+ S V++NQ+D AAIL+SSGTTGRVKGV L+HRNLIA+ + Q +
Sbjct: 188 RSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNY 247
Query: 252 RTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVL 311
V LF LPLFHVFGF MMIR IS G+TLVL+ RF+ E M +AVEKY VT +PVSPPL++
Sbjct: 248 DRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPLIV 307
Query: 312 ALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTL 371
AL KSE KYDL SL+ LGCGGAPLGK++ E+F++KFP+V+I+QGYGLTES+ AA T
Sbjct: 308 ALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAASTF 367
Query: 372 GPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAET 431
GPEE SVGR+SE+MEAKIVDP++GE+LPPG GELWLRGP IMKGYV ++KA+AET
Sbjct: 368 GPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAET 427
Query: 432 LHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIP 491
+ EGWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SNP++IDAAV+P
Sbjct: 428 VDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVP 487
Query: 492 YPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINA 551
+PDE+AGEIPMA++VRKPGSN++E Q+IDF+AK QV PYKK+RRV+FINA
Sbjct: 488 FPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAK-----------QVTPYKKVRRVAFINA 547
Query: 552 IPKSPAGKILRRELVSHALSQGSNKL 566
IPK+PAGKILRREL A+ ++KL
Sbjct: 548 IPKNPAGKILRRELTKIAVDGNASKL 562
BLAST of MS019945 vs. ExPASy Swiss-Prot
Match:
Q7F1X5 (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)
HSP 1 Score: 566.2 bits (1458), Expect = 4.1e-160
Identity = 307/575 (53.39%), Postives = 392/575 (68.17%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADR P +D RSGFC T+IFHS R P LPP S P++ +A SL+ SS P
Sbjct: 1 MADRAPPPPPPPAGIDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
ALVD +G+ +SY L +R+L+ L L G VA ++AP+ L+VPVL FAL
Sbjct: 61 GR-PALVDAATGIAISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSM-M 180
+SIG +SPANP + E AHQV LS+PV+AFA AAKLP + ++I S E+ +
Sbjct: 121 MSIGAVVSPANPVSTPEEYAHQVALSRPVVAFAAPEVAAKLPEHVRCVVIGSDEYGRLAA 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQ----- 240
++ R+ A V + Q+D+AA+LYSSGTTGRVK V ++HRNLIA S A E+
Sbjct: 181 SDGRRAAAPAAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVAREA 240
Query: 241 ----GEIEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTY 300
E P V+L +PLFHVFGF M++R +S G+T VLM+RFDF LRA+E+Y VT
Sbjct: 241 AEAGEEPPPPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVTL 300
Query: 301 IPVSPPLVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTE 360
+P +PP+++A+ K E+ + DLSSL +G GGAPLG+EV E+F FPNVE++QGYGLTE
Sbjct: 301 LPAAPPVLVAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLTE 360
Query: 361 STAAAARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYV 420
S+ A A T+GPEE SVG+L ++AKIVDP++ GYV
Sbjct: 361 SSGAVAATVGPEESKAYGSVGKLGSHLQAKIVDPST---------------------GYV 420
Query: 421 EDDKATAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNP 480
DD+ATA T+ EGWLKTGDLCYF+ DGFLYIVDRLKELIKYK YQVPPAELE +LQS+P
Sbjct: 421 GDDEATAATVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSHP 480
Query: 481 EIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKK 540
I DAAVIPYPDEEAGE+PMA++VR+PGSNI+++QV+D++AK QVAPYKK
Sbjct: 481 GIADAAVIPYPDEEAGELPMAFIVRQPGSNITKEQVMDYVAK-----------QVAPYKK 540
Query: 541 IRRVSFINAIPKSPAGKILRRELVSHALSQGSNKL 566
+RRV+F+ AIPKSPAGKILRRELV ALS G++KL
Sbjct: 541 VRRVAFVTAIPKSPAGKILRRELVQQALSMGASKL 542
BLAST of MS019945 vs. ExPASy Swiss-Prot
Match:
Q8RU95 (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)
HSP 1 Score: 552.7 bits (1423), Expect = 4.7e-156
Identity = 298/567 (52.56%), Postives = 383/567 (67.55%), Query Frame = 0
Query: 18 RSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPP---PANTTALVDFDSGVH 77
RSGFC T+ FHSLR LPP PL++ +A SL+ S+PP ALVD +G+
Sbjct: 41 RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100
Query: 78 VSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVTISPANPTG 137
VSY + +R L+ L L G VA +++P+ L V VLYFAL+SIGV +SPANP
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160
Query: 138 SESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMTESNRSDGVAD--VK 197
+ E AHQV+LS+P IAF AA+LPR + +++I S F + + S A V
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPRHVSRVVIGSEVFDRLASASAAGGWAAPPAVA 220
Query: 198 INQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIE-------------- 257
+ Q +AA+LYSSGTTGRVK V ++HRNLIA S ++ E E
Sbjct: 221 MKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPPPS 280
Query: 258 ---PRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSP 317
P V+LF LPLFHV GF ++ R IS G+T V+M+RFD RAVE+Y VT + +P
Sbjct: 281 PSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSAAP 340
Query: 318 PLVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAA 377
P+V+AL KS++ + DLSSL + GGAPLG+EV ++F FP+V+I+Q YGLTEST
Sbjct: 341 PVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTGPV 400
Query: 378 ARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKA 437
A GPEE + SVGRL+ ++AKIVD A+GE L PG RGELW+RGP +MKGYV D +A
Sbjct: 401 ATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDPEA 460
Query: 438 TAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDA 497
TA T+ P+GWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELE +LQS PEI DA
Sbjct: 461 TAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIADA 520
Query: 498 AVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVS 557
AV+PYPDEEAG++PMA+VVR+PG+ ++E QV++ +AK VAPYKK+RRV+
Sbjct: 521 AVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAK-----------HVAPYKKVRRVA 580
Query: 558 FINAIPKSPAGKILRRELVSHALSQGS 563
F+NAIPKSPAGKILRRELV A++ S
Sbjct: 581 FVNAIPKSPAGKILRRELVLQAMASTS 596
BLAST of MS019945 vs. ExPASy Swiss-Prot
Match:
Q69RG7 (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)
HSP 1 Score: 483.8 bits (1244), Expect = 2.7e-135
Identity = 270/554 (48.74%), Postives = 368/554 (66.43%), Query Frame = 0
Query: 18 RSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLV-------QSSPPPANTTALVDFD 77
R+G+C TK F SLRPP+ LPP PLS + A SL+ SS PAN ALVD
Sbjct: 16 RTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPAN-PALVDAA 75
Query: 78 SGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVTISPA 137
+G VS+ L +R L+ L++ L G VAF+LAP L VPVLYFALLSIG +SPA
Sbjct: 76 TGEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPA 135
Query: 138 NPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGK-ILIDSPEFLSMMTESNRSDG-- 197
NP + +E++ V LS +AFA SSTA KLP L +L+DSP F S++ + ++ G
Sbjct: 136 NPALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQE 195
Query: 198 -VADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPRTVSLFL 257
+ V + Q+++AAI YSSGTTGRVK L HR+ IA + +L + E RT L
Sbjct: 196 PLPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAR-EVRT--LLG 255
Query: 258 LPLFHVFGFYMMIRGISRGDTLVL----MQRFDFEGMLRAVEKYMVTYIPVSPPLVLALA 317
P+FH GF +++G++ G T V+ + R G++ A E++ V + SPP+VL +
Sbjct: 256 APMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVVLGMT 315
Query: 318 KSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLGPE 377
K + L +L+ + CGGAPL +E+F+ +FP+V++ GYG TE+ +R + E
Sbjct: 316 KQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEA-GGISRMISQE 375
Query: 378 ECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETLHP 437
EC++ S GR++E++E KIVD +G+ LP G +GELW+RGP +M GYV D++A A T +
Sbjct: 376 ECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANATTFNS 435
Query: 438 EGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPYPD 497
EGWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELEL+L S P+I+DAAV+PYP
Sbjct: 436 EGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVMPYPH 495
Query: 498 EEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINAIPK 557
EEAG+IP+A VV++PGS ++E +V+ +AK QVAPYKKIR+V F+++IPK
Sbjct: 496 EEAGQIPVALVVKQPGSKLTEAEVMYNVAK-----------QVAPYKKIRKVLFVDSIPK 549
BLAST of MS019945 vs. ExPASy Swiss-Prot
Match:
M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)
HSP 1 Score: 460.3 bits (1183), Expect = 3.1e-128
Identity = 261/570 (45.79%), Postives = 353/570 (61.93%), Query Frame = 0
Query: 9 NSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQ--SSPPPANTTAL 68
NS+ VD RSG+C IF+S R P+ LP +H++ + SS A
Sbjct: 3 NSSKAIVDERSGYCKSNSIFYSKREPVQLP--------QNHSVDVTTFISSRAHHGKIAF 62
Query: 69 VDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVT 128
+D +G H+++ L R + +++ L A+ + G V +L+P S+ PV+ A++S+G
Sbjct: 63 IDAATGRHLTFPQLWRAVDSVATCLSAM-GIRKGDVILLLSPNSIYFPVVCLAVMSLGAI 122
Query: 129 ISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLP-RKLGKILIDSP------------ 188
I+ NP + EIA Q+ SKPV+AF +K+ L ++ID
Sbjct: 123 ITTTNPLNTPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIV 182
Query: 189 EFLSMMTESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVE 248
L M S ++NQ D+A +LYSSGTTG KGV SH+NLIA T+ S
Sbjct: 183 SSLGEMMRKEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILS--R 242
Query: 249 QGEIEPRTVSLFLLPLFHVFGFYMMIRG-ISRGDTLVLMQRFDFEGMLRAVEKYMVTYIP 308
G + + +P+FH++G G +S G T+V++ +F+ ML A+EKY TY+P
Sbjct: 243 FGTEDGEHTFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLP 302
Query: 309 VSPPLVLALAKSEQ--VAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTE 368
+ PP+++AL K+ AKYDLSSLQ + GGAPL KEV+E F E +P V I+QGYGLTE
Sbjct: 303 LVPPILMALLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTE 362
Query: 369 STAAAARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYV 428
ST A T +E + G LS SMEAKIV+P +GEAL GELWLRGP IMKGY
Sbjct: 363 STGIGASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYF 422
Query: 429 EDDKATAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNP 488
+++AT+ T+ EGWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL S+P
Sbjct: 423 SNEEATSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHP 482
Query: 489 EIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKK 548
EI DAAVIPYPD+EAG+ PMAYVVRK GSN+SE V+DFIAK VAPYK+
Sbjct: 483 EISDAAVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAK-----------LVAPYKR 542
Query: 549 IRRVSFINAIPKSPAGKILRRELVSHALSQ 561
IR+V+F+ +IPK+P+GKILR++L+ A S+
Sbjct: 543 IRKVAFVASIPKNPSGKILRKDLIKLATSK 550
BLAST of MS019945 vs. ExPASy TrEMBL
Match:
A0A6J1CAL9 (4-coumarate--CoA ligase-like 9 OS=Momordica charantia OX=3673 GN=LOC111008965 PE=4 SV=1)
HSP 1 Score: 1066.2 bits (2756), Expect = 4.6e-308
Identity = 553/565 (97.88%), Postives = 553/565 (97.88%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPI QPLSITDHALSLVQSSPPP
Sbjct: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPIPQPLSITDHALSLVQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL
Sbjct: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT 180
LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT
Sbjct: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT 180
Query: 181 ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR 240
ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR
Sbjct: 181 ESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPR 240
Query: 241 TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA 300
TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA
Sbjct: 241 TVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLA 300
Query: 301 LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG 360
LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG
Sbjct: 301 LAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG 360
Query: 361 PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL 420
PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL
Sbjct: 361 PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL 420
Query: 421 HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY 480
HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY
Sbjct: 421 HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY 480
Query: 481 PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINAI 540
PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAK QVAPYKKIRRVSFINAI
Sbjct: 481 PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAK-----------QVAPYKKIRRVSFINAI 540
Query: 541 PKSPAGKILRRELVSHALSQGSNKL 566
PKSPAGKILRRELVSHALSQGSNKL
Sbjct: 541 PKSPAGKILRRELVSHALSQGSNKL 554
BLAST of MS019945 vs. ExPASy TrEMBL
Match:
A0A6J1EA56 (4-coumarate--CoA ligase-like 9 OS=Cucurbita moschata OX=3662 GN=LOC111431280 PE=4 SV=1)
HSP 1 Score: 890.2 bits (2299), Expect = 4.5e-255
Identity = 460/569 (80.84%), Postives = 505/569 (88.75%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNP+LN AAHFVD RSGFCPQTKIFHSLRPPL +PP+SQPLS+T+HALSL+QSSPPP
Sbjct: 1 MADRNPSLN-AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
+N LVD +SGVH+SYA+ LR+IR L++NLKALTSLS+GQVAFILAPTSL+VPVLYFAL
Sbjct: 61 SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV ISPANP GS+SEI+HQ++LSKPVIAFATSSTA+KLP+ +LG +LIDSP FLSMM
Sbjct: 121 LSLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVEQGE 240
TE NRSDG+ADVK++Q+DSAAILYSSGTTGRVKGV LSHRNLI NS+ L S V GE
Sbjct: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
EPR VSL LLPLFHVFGF M++R ISRG+TLVLMQ+FDF ML AVEK+ VTYIPVSPP
Sbjct: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P VEIIQGYGLTESTA AA
Sbjct: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
R++GPEE SNT SVGRLSE+MEA IVDP SGEALPPGHRGELWLRGPGIMKGYV D KAT
Sbjct: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETLHP+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDE+AGEIPMAYVVRKPGSNI+E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HA S GSNKL
Sbjct: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 557
BLAST of MS019945 vs. ExPASy TrEMBL
Match:
A0A6J1KJC1 (4-coumarate--CoA ligase-like 9 OS=Cucurbita maxima OX=3661 GN=LOC111495139 PE=4 SV=1)
HSP 1 Score: 885.2 bits (2286), Expect = 1.5e-253
Identity = 458/569 (80.49%), Postives = 504/569 (88.58%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNP+LN AAHFVD RSGFCPQTKIF SLRPPL +PP+SQPLS+T+HALSL+QSSPPP
Sbjct: 1 MADRNPSLN-AAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
+N LVD +SGVH+SYA+ LR+IR L++NLKALTSLS+GQVAFILAPTSL+VPVLYFAL
Sbjct: 61 SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV ISPANP GS+SEI+HQ++LS+PVIAFATSSTA+KLP+ +LG +LIDSP FLSMM
Sbjct: 121 LSLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMM 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVEQGE 240
TE NRSDG+ADVK++Q+DSAAILYSSGTTGRVKGV LSHRNLI NS+ L S V GE
Sbjct: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
EPR VSL LLPLFHVFGF M++R ISRG+TLVLMQ+FDF ML AVEK+ VTYIPVSPP
Sbjct: 241 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+ALAKSE VAKYDLSSLQ LGCGGAPLGKEV+EKF +K P VEIIQGYGLTESTA AA
Sbjct: 301 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
R++GPEE SNT SVGRLSE+MEA IVDPASGEALPPGHRGELWLRGPGIMKGYV D AT
Sbjct: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETLHP+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDE+AGEIPMAYVVRKPGSN++E QVIDFIAK QVAPYKKIRRVSF
Sbjct: 481 VIPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAK-----------QVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HA S GSNKL
Sbjct: 541 IDAIPKSPAGKILRRELTQHARSHGSNKL 557
BLAST of MS019945 vs. ExPASy TrEMBL
Match:
A0A6J1C6X8 (4-coumarate--CoA ligase-like 9 OS=Momordica charantia OX=3673 GN=LOC111008964 PE=4 SV=1)
HSP 1 Score: 881.7 bits (2277), Expect = 1.6e-252
Identity = 459/572 (80.24%), Postives = 501/572 (87.59%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MADRNPN +AAH VDPRSGFCPQTKIFHSLRPPLSLPPI QPLS+ +HAL+L+QSSPPP
Sbjct: 1 MADRNPNF-TAAHSVDPRSGFCPQTKIFHSLRPPLSLPPIPQPLSVIEHALTLLQSSPPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
ANT+ LVD +SGV VSY+L +R+IRNL++NL+ALTSLS+GQVAFILAPTSLQVPVL FAL
Sbjct: 61 ANTSVLVDSNSGVRVSYSLFIRQIRNLASNLQALTSLSNGQVAFILAPTSLQVPVLLFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEFLSMMT 180
LS+GVTISPANPTGS SEIAHQV+LSKPVIAFATSSTA KLPR+L KILIDSPEF SM+
Sbjct: 121 LSLGVTISPANPTGSHSEIAHQVRLSKPVIAFATSSTAPKLPRELAKILIDSPEFFSMII 180
Query: 181 ESNRS----DGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANST---LASLVE 240
ES RS DGVA VK NQ+DSAAILYSSGTTG+VKGV L+HRNL+AAN+ +V
Sbjct: 181 ESKRSGGVDDGVAQVKTNQSDSAAILYSSGTTGQVKGVLLTHRNLVAANAVAHGFQLMVS 240
Query: 241 QGEIEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPV 300
E E V L LLPLFHVFGF M+IR ISRGDT+VLMQ+FDF GMLRAVEKYMVTYI V
Sbjct: 241 SKEKERHPVFLCLLPLFHVFGFIMLIRAISRGDTVVLMQKFDFGGMLRAVEKYMVTYISV 300
Query: 301 SPPLVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTA 360
+PPLV+ALAKSE KYDLSSL+ LGCGGAPLG+E++EKF EKFPNVEI QGYGLTESTA
Sbjct: 301 APPLVVALAKSELKVKYDLSSLRILGCGGAPLGREIIEKFHEKFPNVEITQGYGLTESTA 360
Query: 361 AAARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDD 420
A+RTLG EE SNTSSVGRL ESMEAKIVDPASGEAL PGH+GELWLRGPGIMKGYV DD
Sbjct: 361 GASRTLGSEESSNTSSVGRLCESMEAKIVDPASGEALSPGHKGELWLRGPGIMKGYVGDD 420
Query: 421 KATAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEII 480
KATAETLHPEGWL+TGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEI+
Sbjct: 421 KATAETLHPEGWLRTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIV 480
Query: 481 DAAVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRR 540
DAAVIPYPDEEAGEIPMAYVVRKPGSNI+E QVIDF+AK QVAPYKKIRR
Sbjct: 481 DAAVIPYPDEEAGEIPMAYVVRKPGSNITEAQVIDFVAK-----------QVAPYKKIRR 540
Query: 541 VSFINAIPKSPAGKILRRELVSHALSQGSNKL 566
SFINA+PKSP+GKILRRELV HALS GS+KL
Sbjct: 541 ASFINAVPKSPSGKILRRELVKHALSHGSHKL 560
BLAST of MS019945 vs. ExPASy TrEMBL
Match:
A0A5D3BKJ5 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004520 PE=4 SV=1)
HSP 1 Score: 865.1 bits (2234), Expect = 1.6e-247
Identity = 441/569 (77.50%), Postives = 497/569 (87.35%), Query Frame = 0
Query: 1 MADRNPNLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPP 60
MA RN N N+A H +D RSGFCP TKIFHSLRPPLSLPP+SQPLS+T HA SL+QSS PP
Sbjct: 1 MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60
Query: 61 ANTTALVDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFAL 120
N+T L+D +SG+H+SYA+ LR+IRNL++NLK+LTSL +G VAFILAPTSLQ+PVLYFAL
Sbjct: 61 PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120
Query: 121 LSIGVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPR-KLGKILIDSPEFLSMM 180
LS+GV +SPANPT S SEI+HQ+QLSKPVIAFATSSTA+KLP + G ++IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180
Query: 181 TESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANS---TLASLVEQGE 240
TE+N SDG+ D+KI+Q+DSAAILYSSGTTGRVKGV LSHRNLIA NS S + +GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240
Query: 241 IEPRTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPP 300
++P V+L LLPLFHVFGF M++R ISRG+TLVLM+RF+FEGMLRAVEK+ V YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300
Query: 301 LVLALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAA 360
LV+A+AKS+ VAKYDLSSLQ LGCGGAPLGKEV++KF +K P+VEI QGYGLTESTA AA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360
Query: 361 RTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKAT 420
RT+ PEE SNT SVGRLS SMEAKIVDPASGEAL P H+GELWLRGP IMKGYV DDKAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420
Query: 421 AETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAA 480
ETL P+GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELE LLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
Query: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSF 540
VIPYPDEEAGEIPMAYVVRKPGSNISE QVIDFIAKQA + + VAPYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540
Query: 541 INAIPKSPAGKILRRELVSHALSQGSNKL 566
I+AIPKSPAGKILRREL HALS GS+KL
Sbjct: 541 IDAIPKSPAGKILRRELAKHALSHGSSKL 569
BLAST of MS019945 vs. TAIR 10
Match:
AT5G63380.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 665.6 bits (1716), Expect = 3.5e-191
Identity = 348/566 (61.48%), Postives = 431/566 (76.15%), Query Frame = 0
Query: 12 AHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANT-------T 71
+ +D SGF +T I+HSLRP LSLPPI QPLS + ALSL+ S PPA T
Sbjct: 8 SRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALT 67
Query: 72 ALVDFDSGVHVSYALLLREIRNLSANLK-ALTSLSSGQVAFILAPTSLQVPVLYFALLSI 131
LV+ SG +++Y LLR +R+L+ +L+ SL+S VAFIL+P+SL +PVLY AL+SI
Sbjct: 68 YLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSI 127
Query: 132 GVTISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRK---LGKILIDSPEFLSMMT 191
GV +SPANP GSESE++HQV++S+PVIAFATS T KL LG +L+DS EFLS +
Sbjct: 128 GVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLN 187
Query: 192 ESNRSD-GVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEP 251
S+ S V++NQ+D AAIL+SSGTTGRVKGV L+HRNLIA+ + Q +
Sbjct: 188 RSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNY 247
Query: 252 RTVSLFLLPLFHVFGFYMMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVL 311
V LF LPLFHVFGF MMIR IS G+TLVL+ RF+ E M +AVEKY VT +PVSPPL++
Sbjct: 248 DRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPPLIV 307
Query: 312 ALAKSEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTL 371
AL KSE KYDL SL+ LGCGGAPLGK++ E+F++KFP+V+I+QGYGLTES+ AA T
Sbjct: 308 ALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAASTF 367
Query: 372 GPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAET 431
GPEE SVGR+SE+MEAKIVDP++GE+LPPG GELWLRGP IMKGYV ++KA+AET
Sbjct: 368 GPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAET 427
Query: 432 LHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIP 491
+ EGWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SNP++IDAAV+P
Sbjct: 428 VDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVP 487
Query: 492 YPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINA 551
+PDE+AGEIPMA++VRKPGSN++E Q+IDF+AK QV PYKK+RRV+FINA
Sbjct: 488 FPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAK-----------QVTPYKKVRRVAFINA 547
Query: 552 IPKSPAGKILRRELVSHALSQGSNKL 566
IPK+PAGKILRREL A+ ++KL
Sbjct: 548 IPKNPAGKILRRELTKIAVDGNASKL 562
BLAST of MS019945 vs. TAIR 10
Match:
AT1G20510.1 (OPC-8:0 CoA ligase1 )
HSP 1 Score: 450.7 bits (1158), Expect = 1.8e-126
Identity = 246/559 (44.01%), Postives = 356/559 (63.69%), Query Frame = 0
Query: 15 VDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANTTALVDFDSGVH 74
V+ RSGFC F+S R P+ LPP + L +T SS A +D +G +
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63
Query: 75 VSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVTISPANPTG 134
+++ L R + +++ L + + G V +L+P S+ PV+ +++S+G I+ NP
Sbjct: 64 LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123
Query: 135 SESEIAHQVQLSKPVIAFATSSTAAKL---PRKLGKILIDSPEFLSM--------MTESN 194
+ +EIA Q++ S PV+AF TS K+ +KL +L+D S+ M +
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183
Query: 195 RSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPRTVS 254
S +++Q D+A +LYSSGTTG KGV SHRNLIA T+ + + E R +
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFIC 243
Query: 255 LFLLPLFHVFGFYMMIRG-ISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLALA 314
+P+FH++G G ++ G T++++ +F+ M+ A+ KY T +P+ PP+++A+
Sbjct: 244 --TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMV 303
Query: 315 K-SEQV-AKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTLG 374
++Q+ AKYDLSS+ + CGGAPL KEV E F EK+P V+I+QGYGLTEST A T
Sbjct: 304 NGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDT 363
Query: 375 PEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAETL 434
EE + G+LS SME +IVDP +G+ L P GELWL+GP IMKGY +++AT+ TL
Sbjct: 364 VEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTL 423
Query: 435 HPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIPY 494
EGWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAAVIP+
Sbjct: 424 DSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPF 483
Query: 495 PDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINAI 554
PD+E G+ PMAYVVRK GS++SE +++F+AK QVAPYK+IR+V+F+++I
Sbjct: 484 PDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAK-----------QVAPYKRIRKVAFVSSI 542
Query: 555 PKSPAGKILRRELVSHALS 560
PK+P+GKILR++L+ A S
Sbjct: 544 PKNPSGKILRKDLIKIATS 542
BLAST of MS019945 vs. TAIR 10
Match:
AT1G20500.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-125
Identity = 250/561 (44.56%), Postives = 347/561 (61.85%), Query Frame = 0
Query: 15 VDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANTTALVDFDSGVH 74
VDPRSGFC F+S R PLSLPP +++ + + SS P TA +D +G
Sbjct: 10 VDPRSGFCKSNSTFYSKRQPLSLPP-----NLSRDVTTFI-SSQPHRGKTAFIDAATGQC 69
Query: 75 VSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVTISPANPTG 134
++++ L R + ++ L + G V IL+P S+ +PV+ +++S+G + AN
Sbjct: 70 LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 129
Query: 135 SESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPEF------------LSMMTES 194
+ EI+ Q+ S P + F T A KLP + +L D + LS M +
Sbjct: 130 TSGEISKQIADSNPTLVFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKK 189
Query: 195 NRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGEIEPRTV 254
S ++NQ D+A +LYSSGTTG KGV SHRNL A + S ++ +
Sbjct: 190 EPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS----DNLKRDDI 249
Query: 255 SLFLLPLFHVFGFYMMIRG-ISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSPPLVLAL 314
+ +P+FH +G G ++ G T+V+++RF M+ AVEK+ T + ++PP+++A+
Sbjct: 250 FICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAM 309
Query: 315 AKSEQV--AKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTAAAARTL 374
+ AKYDLSSL+ + CGGAPL KEV E F EK+P V+I+QGY LTES A T
Sbjct: 310 INDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTN 369
Query: 375 GPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDDKATAET 434
EE + G L+ +EA+IVDP +G + GELWL+GP I KGY ++ +AT ET
Sbjct: 370 SAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNET 429
Query: 435 LHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEIIDAAVIP 494
++ EGWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++P+I+DAAVIP
Sbjct: 430 INLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIP 489
Query: 495 YPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRRVSFINA 554
+PD+EAG+ PMAYVVRK SN+SE QVIDFI+K QVAPYKKIR+VSFIN+
Sbjct: 490 FPDKEAGQYPMAYVVRKHESNLSEKQVIDFISK-----------QVAPYKKIRKVSFINS 549
Query: 555 IPKSPAGKILRRELVSHALSQ 561
IPK+ +GK LR++L+ A S+
Sbjct: 550 IPKTASGKTLRKDLIKLATSK 549
BLAST of MS019945 vs. TAIR 10
Match:
AT1G20480.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-121
Identity = 253/567 (44.62%), Postives = 350/567 (61.73%), Query Frame = 0
Query: 15 VDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANTTALVDFDSGVH 74
VD +SGFC T IF+S R P++LPP +Q L +T +S P T VD +G
Sbjct: 21 VDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVTGRR 80
Query: 75 VSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVTISPANPTG 134
+S+ L + ++ L AL + G V IL+P S+ P++ +++S+G I+ ANP
Sbjct: 81 LSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 140
Query: 135 SESEIAHQVQLSKPVIAFATSSTAAKLPR----KLGKILIDSPEF--------------L 194
+ EI+ Q+ S+PV+AF T +KL L +L+D L
Sbjct: 141 TSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRL 200
Query: 195 SMMTESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVEQGE 254
M E+ S+ ++NQ D+AA+LYSSGTTG KGV LSHRNLIA + + +
Sbjct: 201 ETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA---LVQAYRARFG 260
Query: 255 IEPRTVSLFLLPLFHVFGFYMMIRG-ISRGDTLVLMQRFDFEGMLRAVEKYMVTYIPVSP 314
+E RT+ +P+ H+FGF G I+ G T+V++ +FD +L AVE + +Y+ + P
Sbjct: 261 LEQRTIC--TIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVP 320
Query: 315 PLVLALAK--SEQVAKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTESTA 374
P+V+A+ +E +KYDLSSL + GGAPL +EV EKF E +P V+I+QGYGLTESTA
Sbjct: 321 PIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTA 380
Query: 375 AAARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYVEDD 434
AA EE + G L+ ++E KIVDP +G L GELW+R P +MKGY ++
Sbjct: 381 IAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNK 440
Query: 435 KATAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNPEII 494
+ATA T+ EGWLKTGDLCY D DGF+++VDRLKELIK YQV PAELE LL ++PEI
Sbjct: 441 EATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIA 500
Query: 495 DAAVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKKIRR 554
DAAVIP PD +AG+ PMAY+VRK GSN+SE +++ F+AK QV+PYKKIR+
Sbjct: 501 DAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAK-----------QVSPYKKIRK 560
Query: 555 VSFINAIPKSPAGKILRRELVSHALSQ 561
V+F+ +IPK+P+GKILRREL S+
Sbjct: 561 VTFLASIPKNPSGKILRRELTKLTTSK 564
BLAST of MS019945 vs. TAIR 10
Match:
AT5G38120.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 423.3 bits (1087), Expect = 3.0e-118
Identity = 242/570 (42.46%), Postives = 349/570 (61.23%), Query Frame = 0
Query: 7 NLNSAAHFVDPRSGFCPQTKIFHSLRPPLSLPPISQPLSITDHALSLVQSSPPPANTTAL 66
N ++ +DPR+GFC F+S R PL+LP + L IT SS TA
Sbjct: 3 NSQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTAF 62
Query: 67 VDFDSGVHVSYALLLREIRNLSANLKALTSLSSGQVAFILAPTSLQVPVLYFALLSIGVT 126
+D + +S++ L + ++ L + G V +L+P ++ +P++ +++S+G
Sbjct: 63 IDAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAV 122
Query: 127 ISPANPTGSESEIAHQVQLSKPVIAFATSSTAAKLPRKLGKILIDSPE------------ 186
++ ANP + SEI Q+ S P +AF T A K+ I+++ E
Sbjct: 123 LTTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVV 182
Query: 187 -FLSMMTESNRSDGVADVKINQTDSAAILYSSGTTGRVKGVFLSHRNLIAANSTLASLVE 246
L+ M + S ++++ D+A +LYSSGTTGR KGV SH NLIA +A +
Sbjct: 183 GNLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA---HVARYIA 242
Query: 247 QGEIEPRTVSLFLLPLFHVFGFY-MMIRGISRGDTLVLMQRFDFEGMLRAVEKYMVTYIP 306
+ +P+ + +PLFH FG ++ ++ G T+V++ RFD M+ AVEKY T +
Sbjct: 243 EPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATTLI 302
Query: 307 VSPP-LVLALAKSEQV-AKYDLSSLQFLGCGGAPLGKEVVEKFQEKFPNVEIIQGYGLTE 366
+ PP LV + K++Q+ KYD+S L+ + CGGAPL KEV + F +K+P V++ QGY LTE
Sbjct: 303 LVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTE 362
Query: 367 STAAAARTLGPEECSNTSSVGRLSESMEAKIVDPASGEALPPGHRGELWLRGPGIMKGYV 426
S A A EE +VG LS +EA+IVDP +G+ + GELWL+GP I KGY
Sbjct: 363 SNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKGYF 422
Query: 427 EDDKATAETLHPEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELELLLQSNP 486
+++ E + EGWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL ++P
Sbjct: 423 RNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHP 482
Query: 487 EIIDAAVIPYPDEEAGEIPMAYVVRKPGSNISEDQVIDFIAKQAYSNSTNFILQVAPYKK 546
+I+DAAVIP+PD+EAG+ PMAYV RKP SN+ E +VIDFI+K QVAPYKK
Sbjct: 483 DILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISK-----------QVAPYKK 542
Query: 547 IRRVSFINAIPKSPAGKILRRELVSHALSQ 561
IR+V+FI++IPK+P+GK LR++L+ A+S+
Sbjct: 543 IRKVAFIDSIPKTPSGKTLRKDLIKFAISK 549
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022137541.1 | 9.5e-308 | 97.88 | 4-coumarate--CoA ligase-like 9 [Momordica charantia] >XP_022137542.1 4-coumarate... | [more] |
| XP_022923628.1 | 9.4e-255 | 80.84 | 4-coumarate--CoA ligase-like 9 [Cucurbita moschata] | [more] |
| XP_038894373.1 | 2.7e-254 | 80.49 | 4-coumarate--CoA ligase-like 9 [Benincasa hispida] | [more] |
| KAG6584140.1 | 4.7e-254 | 80.67 | 4-coumarate--CoA ligase-like 9, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| KAG7019731.1 | 4.7e-254 | 80.67 | 4-coumarate--CoA ligase-like 9 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q84P23 | 4.9e-190 | 61.48 | 4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=... | [more] |
| Q7F1X5 | 4.1e-160 | 53.39 | 4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5... | [more] |
| Q8RU95 | 4.7e-156 | 52.56 | 4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6... | [more] |
| Q69RG7 | 2.7e-135 | 48.74 | 4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7... | [more] |
| M4IQR7 | 3.1e-128 | 45.79 | Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CAL9 | 4.6e-308 | 97.88 | 4-coumarate--CoA ligase-like 9 OS=Momordica charantia OX=3673 GN=LOC111008965 PE... | [more] |
| A0A6J1EA56 | 4.5e-255 | 80.84 | 4-coumarate--CoA ligase-like 9 OS=Cucurbita moschata OX=3662 GN=LOC111431280 PE=... | [more] |
| A0A6J1KJC1 | 1.5e-253 | 80.49 | 4-coumarate--CoA ligase-like 9 OS=Cucurbita maxima OX=3661 GN=LOC111495139 PE=4 ... | [more] |
| A0A6J1C6X8 | 1.6e-252 | 80.24 | 4-coumarate--CoA ligase-like 9 OS=Momordica charantia OX=3673 GN=LOC111008964 PE... | [more] |
| A0A5D3BKJ5 | 1.6e-247 | 77.50 | 4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... | [more] |
