MS019939 (gene) Bitter gourd (TR) v1

Overview
NameMS019939
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCitrulline--aspartate ligase
Locationscaffold22: 670220 .. 673081 (+)
RNA-Seq ExpressionMS019939
SyntenyMS019939
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCATTCGTGGCTGCAGCTGGGTGCGAAAACATGTGAAATTCATGGCAACAGCTTAGCTAGTAACAGGGCTAGTATTGTGCGATCTAATAAGAAAGGCGGTGAGTTTCCTCTTGTTGCTTGCTCTGCGTTTAATCTCCCTTTTTCTTTTGTAGTTCTGTTCACAACTTCTAAGCGGGCTGGAGAAGTTGGGGGTTTTTTGTTTATCATATCGAGCTTTTAATCATGGGATTACTCTGTTACGTACCGTTTTAATAGATAATAATTTTGTTTGTAGTTGCGTTTATATTTTATTTTATTATAAAAGATTGGTTTGTGGTGTATTTTTTGTTAGTAATTTTGTTCTACTTGTTTTAGTTCTGTGATCTAATGCTTTATAAAACGTGCTTTTGTGTTATTCCCTACGCATCGAGTTTGTGAACATTTTTGTCCTTTCTTTTTGAGACTTCAATTATTGTTGAATACAGTTATTCAGGCAATACTGCAGAGTGACAGAGAGATAGAAACAACCAACGGTGTTGGGACTAGAGGTCTGCGGGGGAAATTAAATAAGGTTGTTCTGGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGTAGGTTTCCATTTTTCTAATGACCCAGAGCAGAATCTATAAATCTGCATTCGCAAGAACTATTCGCTAAGTTGGAATGTCTGTGCATTTGACTTTTTGTGAGTTCCAAGCCGTCCCTTTTGCTTGTACTGCCTGGATTTTGCTTTGAGCTCTAGTCCACGTCGTGCATTCTCCTTCTGCATTCATCTCCGGGGATTTGAGCAGCCCTTTTTAGTATCTCATTCACGGCTTCTGAATTATTTCTTCTTATGGGAATTAATTATTGTTTGTATTGCAGGGAGAATTATGGTTGTGAAGTCGTATGTTTTACTGCAGATGTCGGTCAAGTATGTAATTAGCATAGGTGAATTTGTTTTGTGGGGGGTTCTTTGTTGTTTTTTAATATTCCACTTGAGGAAACCTTATGAAAGTGATCTTTATGCATCTCTTTCTGGGCCTCAATTTGTAGGGCGTAAAGGAACTGGATGGTTTGGAACAGAAGGCTAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGACTTAAAGGAAGAATTTGTGAGAGACTTCATATTTCCTTGCTTACGTGCTGGTGCGATTTATGAAAGAAAATACCTGCTTGGGACTTCAATGGCCCGTCCAGTTATTGCAAAGGTAAGTGCTAACTTTAAGTAATATTTGCGTTGCCACATGGATTACTGAACGATAAATGAATTTATGGTTCTAAGTGACTTGTATGCTCGAATCACATCTATCATGTTCATGGTTTTTACTTGTTGATTTGGTAATTCCTTCCCATTCCATTTTGATATTTGGAAAAAATAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCCGTCTCTCATGGATGTACCGGTAAAGGAAATGACCAGGTATGCCCTTACACCCTGATCTGGATATTCATGTATTCTTTTTATCTGATGAACTGTATTGGTCTGGTATACTTTTTAAGGTACGCTTTGAGTTGACCTTCTTTGCTCTAAATCCCAAACTAAACGTGGTGGCCCCATGGAGGGAATGGGATATTACTGGTAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATGTACCTGTTCCAGTGACAAAGAAATCAATTTATAGTCGAGACCGGAACTTATGGCATCTTAGTCATGAGGTAATCCTCCTTCCTCAGCCTATTTGAATTTTTGATGGTCAAACATTTAGCGATAGATTAACAATTTCAAGTATTTGTTAACCAAAGCATGGGAAACAATGGAAATAATGTGGGGAACTACCTTTCCTAGTGTCAATCCTATGATGTCAAAAAATTTGGTTGCTTTGGCCTCTTTATGAATTTTATCTCTTGCTGATGTTATCCTGCTCATATTATAACTGAAGGGAGACGTTCTGGAGGATCCAGCAAACGAACCTAAAAAGGATATGTACATGATATCTACAGATCCAGAAGATGCACCAAACGAACCCGAGTAAATTTCCTTCTCTCGAAATGCCACGTGTTTACTCTCTCAAATTATTGCCTACTCTCAAACTAGAACTAGAAGTGTAGTAATAGAAGAGTTCAAATAGTATGAAGAGAAGAAGAAATATACCTAAAAGAATCCAATTTCCAAGTTTAAAACTTTTATGTAAAATTAGACAAAAAAATGTTAGCTTTGCTGTAATGTAGTAATACTAATACCTTTGTCTCTTTGGTATTGGGCATTTTCTTCTGCAGGTACGTGGAAATAGGAATTGTCCAGGGTCTCCCCGTCTCTGTGAACGGCGAGATGCTTTCACCAGCATCTCTTCTAACCAAACTAAACGAACTTGGTGGGAAGCACGGAATTGGCAGAATTGACATGGTGGAAAATCGTCTGGTGGGCATGAAAAGCAGAGGCGTGTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTAGAGTCTCTAACACTGGACCGAGAGACGATTCAAGTCAAGGATTCACTGGCCCTCAAATATGCAGAGTTGGTATACGCAGGGAGATGGTTCGACCCTCTCCGCGAATCAATGGACGCTTTCATGGAGAAGATCACAGAGACCACCACCGGCTCCGTCACTCTCAAACTGTACAAAGGCTCCGTCAGTGTGACCAGCCGGCGAAGTATTTACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAGAATGGGCAGATCTACGATCAGGCCGATGCCGCCGGCTTCATCCGCCTTTACGGCCTTCCGACCAGGGTCCGGGCAATGCTCCAACAGGGGGTC

mRNA sequence

TTGCATTCGTGGCTGCAGCTGGGTGCGAAAACATGTGAAATTCATGGCAACAGCTTAGCTAGTAACAGGGCTAGTATTGTGCGATCTAATAAGAAAGGCGTTATTCAGGCAATACTGCAGAGTGACAGAGAGATAGAAACAACCAACGGTGTTGGGACTAGAGGTCTGCGGGGGAAATTAAATAAGGTTGTTCTGGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGTGAAGTCGTATGTTTTACTGCAGATGTCGGTCAAGGCGTAAAGGAACTGGATGGTTTGGAACAGAAGGCTAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGACTTAAAGGAAGAATTTGTGAGAGACTTCATATTTCCTTGCTTACGTGCTGGTGCGATTTATGAAAGAAAATACCTGCTTGGGACTTCAATGGCCCGTCCAGTTATTGCAAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCCGTCTCTCATGGATGTACCGGTAAAGGAAATGACCAGGTACGCTTTGAGTTGACCTTCTTTGCTCTAAATCCCAAACTAAACGTGGTGGCCCCATGGAGGGAATGGGATATTACTGGTAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATGTACCTGTTCCAGTGACAAAGAAATCAATTTATAGTCGAGACCGGAACTTATGGCATCTTAGTCATGAGGGAGACGTTCTGGAGGATCCAGCAAACGAACCTAAAAAGGATATGTACATGATATCTACAGATCCAGAAGATGCACCAAACGAACCCGAGTACGTGGAAATAGGAATTGTCCAGGGTCTCCCCGTCTCTGTGAACGGCGAGATGCTTTCACCAGCATCTCTTCTAACCAAACTAAACGAACTTGGTGGGAAGCACGGAATTGGCAGAATTGACATGGTGGAAAATCGTCTGGTGGGCATGAAAAGCAGAGGCGTGTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTAGAGTCTCTAACACTGGACCGAGAGACGATTCAAGTCAAGGATTCACTGGCCCTCAAATATGCAGAGTTGGTATACGCAGGGAGATGGTTCGACCCTCTCCGCGAATCAATGGACGCTTTCATGGAGAAGATCACAGAGACCACCACCGGCTCCGTCACTCTCAAACTGTACAAAGGCTCCGTCAGTGTGACCAGCCGGCGAAGTATTTACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAGAATGGGCAGATCTACGATCAGGCCGATGCCGCCGGCTTCATCCGCCTTTACGGCCTTCCGACCAGGGTCCGGGCAATGCTCCAACAGGGGGTC

Coding sequence (CDS)

TTGCATTCGTGGCTGCAGCTGGGTGCGAAAACATGTGAAATTCATGGCAACAGCTTAGCTAGTAACAGGGCTAGTATTGTGCGATCTAATAAGAAAGGCGTTATTCAGGCAATACTGCAGAGTGACAGAGAGATAGAAACAACCAACGGTGTTGGGACTAGAGGTCTGCGGGGGAAATTAAATAAGGTTGTTCTGGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGTGAAGTCGTATGTTTTACTGCAGATGTCGGTCAAGGCGTAAAGGAACTGGATGGTTTGGAACAGAAGGCTAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGACTTAAAGGAAGAATTTGTGAGAGACTTCATATTTCCTTGCTTACGTGCTGGTGCGATTTATGAAAGAAAATACCTGCTTGGGACTTCAATGGCCCGTCCAGTTATTGCAAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCCGTCTCTCATGGATGTACCGGTAAAGGAAATGACCAGGTACGCTTTGAGTTGACCTTCTTTGCTCTAAATCCCAAACTAAACGTGGTGGCCCCATGGAGGGAATGGGATATTACTGGTAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATGTACCTGTTCCAGTGACAAAGAAATCAATTTATAGTCGAGACCGGAACTTATGGCATCTTAGTCATGAGGGAGACGTTCTGGAGGATCCAGCAAACGAACCTAAAAAGGATATGTACATGATATCTACAGATCCAGAAGATGCACCAAACGAACCCGAGTACGTGGAAATAGGAATTGTCCAGGGTCTCCCCGTCTCTGTGAACGGCGAGATGCTTTCACCAGCATCTCTTCTAACCAAACTAAACGAACTTGGTGGGAAGCACGGAATTGGCAGAATTGACATGGTGGAAAATCGTCTGGTGGGCATGAAAAGCAGAGGCGTGTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTAGAGTCTCTAACACTGGACCGAGAGACGATTCAAGTCAAGGATTCACTGGCCCTCAAATATGCAGAGTTGGTATACGCAGGGAGATGGTTCGACCCTCTCCGCGAATCAATGGACGCTTTCATGGAGAAGATCACAGAGACCACCACCGGCTCCGTCACTCTCAAACTGTACAAAGGCTCCGTCAGTGTGACCAGCCGGCGAAGTATTTACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAGAATGGGCAGATCTACGATCAGGCCGATGCCGCCGGCTTCATCCGCCTTTACGGCCTTCCGACCAGGGTCCGGGCAATGCTCCAACAGGGGGTC

Protein sequence

LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKLNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVKDLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLLTKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYREDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV
Homology
BLAST of MS019939 vs. NCBI nr
Match: XP_022137474.1 (argininosuccinate synthase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 906.7 bits (2342), Expect = 7.8e-260
Identity = 453/458 (98.91%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL
Sbjct: 37  LHSFQELGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFELTFFALNPKLNVVAPWR WDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELTFFALNPKLNVVAPWRGWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDP NEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL
Sbjct: 277 HLSHEGDVLEDPGNEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV
Sbjct: 457 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 494

BLAST of MS019939 vs. NCBI nr
Match: XP_038895641.1 (argininosuccinate synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 860.5 bits (2222), Expect = 6.5e-246
Identity = 422/456 (92.54%), Postives = 442/456 (96.93%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGNS+ASNRA +++ NKK VIQAILQS+REIETTNG+GTRGLRG+L
Sbjct: 37  LHSFQELGAKTCEIHGNSIASNRAGVLQLNKKSVIQAILQSEREIETTNGIGTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQG KEL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGAKELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDPANEPKKDMYM+STDPEDAPNEPEYVEIGIV+GLPVSVNGE+LSPASLL
Sbjct: 277 HLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVRGLPVSVNGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
            K+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 AKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGS TLKLYKGSV V SRRS+YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSATLKLYKGSVHVASRRSLYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           EDISSFENGQIY+QADA GFIRLYGLPTRVRAML +
Sbjct: 457 EDISSFENGQIYNQADADGFIRLYGLPTRVRAMLHK 492

BLAST of MS019939 vs. NCBI nr
Match: XP_023519539.1 (argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.8 bits (2220), Expect = 1.1e-245
Identity = 424/458 (92.58%), Postives = 443/458 (96.72%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAK  EIHGNS+A NRAS++RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 37  LHSFQELGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV EL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRD+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIV GLPVS+NGE+LSPASLL
Sbjct: 277 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TK+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 TKVNEMGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML + +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDI 494

BLAST of MS019939 vs. NCBI nr
Match: KAG6584145.1 (Argininosuccinate synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 423/458 (92.36%), Postives = 443/458 (96.72%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAK  EIHGNS+A NRAS++RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 37  LHSFQELGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV EL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRD+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGD+LEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIV GLPVS+NGE+LSPASLL
Sbjct: 277 HLSHEGDILEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TK+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 TKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML + +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDI 494

BLAST of MS019939 vs. NCBI nr
Match: KAG7019736.1 (Argininosuccinate synthase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 423/458 (92.36%), Postives = 443/458 (96.72%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAK  EIHGNS+A NRAS++RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 35  LHSFQELGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQL 94

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV EL+GLE+KARASGACQLVVK
Sbjct: 95  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVK 154

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRD+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 155 DLKEEFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 214

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW
Sbjct: 215 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 274

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGD+LEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIV GLPVS+NGE+LSPASLL
Sbjct: 275 HLSHEGDILEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLL 334

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TK+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 335 TKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 394

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 395 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYR 454

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML + +
Sbjct: 455 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDI 492

BLAST of MS019939 vs. ExPASy Swiss-Prot
Match: Q9SZX3 (Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g24830 PE=1 SV=3)

HSP 1 Score: 740.0 bits (1909), Expect = 1.7e-212
Identity = 364/451 (80.71%), Postives = 406/451 (90.02%), Query Frame = 0

Query: 6   QLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKLNKVVL 65
           +L  K  ++ GN++ +   +  RS K   I+A+L  D    TT+     GLRGKL KVVL
Sbjct: 43  ELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVL 102

Query: 66  AYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVKDLKEE 125
           AYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KEL+GLEQKA+ASGA QLVVKDL EE
Sbjct: 103 AYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEE 162

Query: 126 FVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVR 185
           FV+DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVR
Sbjct: 163 FVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVR 222

Query: 186 FELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 245
           FELTFF+LNP+L VVAPWREW+I GREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE
Sbjct: 223 FELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 282

Query: 246 GDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLLTKLNE 305
           GD+LEDPANEPKKDMYM+S DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LL +LN 
Sbjct: 283 GDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNT 342

Query: 306 LGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALK 365
           +GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAA +ELESLTLDRE+IQVKD+LALK
Sbjct: 343 IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLALK 402

Query: 366 YAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYREDISS 425
           YAE+VYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVT R+S  SLYR+DISS
Sbjct: 403 YAEMVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISS 462

Query: 426 FENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           FE  +IY+QADAAGFIRLYGLP ++RAML++
Sbjct: 463 FEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of MS019939 vs. ExPASy Swiss-Prot
Match: Q3A9W5 (Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=argG PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 4.3e-152
Identity = 258/395 (65.32%), Postives = 319/395 (80.76%), Query Frame = 0

Query: 62  KVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVKD 121
           KVVLAYSGGLDTS+I+PWL+ENYG EV+   ADVGQG +EL+ L +KA  SGA ++ ++D
Sbjct: 4   KVVLAYSGGLDTSIIIPWLKENYGYEVIAMVADVGQG-EELEPLREKAIKSGAAKIYIED 63

Query: 122 LKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGN 181
           +KEEFVRDF+FP L+AGA+YE KYLLGTS+ARP+IAK +V++A++ GA AV+HG TGKGN
Sbjct: 64  VKEEFVRDFVFPMLKAGAVYENKYLLGTSVARPLIAKKLVEIAEKEGAVAVAHGATGKGN 123

Query: 182 DQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWH 241
           DQVRFELT  ALNP L ++APWREW+I  REDAI+YA+K  +PVPVTKK  YS DRNLWH
Sbjct: 124 DQVRFELTVKALNPDLKIIAPWREWEIKSREDAIDYAEKRGIPVPVTKKQPYSMDRNLWH 183

Query: 242 LSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLLT 301
           LSHEG +LEDPA EP +D+ +++  PE AP++PEYVEI  V+G PV+VNGE LSP  L+ 
Sbjct: 184 LSHEGGILEDPAVEPPEDVLLLTNPPEKAPDQPEYVEIEFVKGEPVAVNGEKLSPVELIF 243

Query: 302 KLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDS 361
           KLNELGGKHGIG  DMVENRLVGMKSRGVYETPGGTIL  A RELESLTLDR+T+  K  
Sbjct: 244 KLNELGGKHGIGIADMVENRLVGMKSRGVYETPGGTILTFAHRELESLTLDRQTMHFKQM 303

Query: 362 LALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYRE 421
           +ALKYAEL+Y G WF PLRE+++AF++K  ET TG V +KLYKG++      S YSLY +
Sbjct: 304 VALKYAELIYDGLWFTPLREALEAFVDKTQETVTGKVRVKLYKGNIYPAGITSPYSLYVK 363

Query: 422 DISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           D+++F    +Y+Q DA GFI L+GLP +VRAM Q+
Sbjct: 364 DLATFGEDNLYNQKDAEGFINLFGLPLKVRAMTQK 397

BLAST of MS019939 vs. ExPASy Swiss-Prot
Match: A5V0J9 (Argininosuccinate synthase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=argG PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.4e-149
Identity = 249/401 (62.09%), Postives = 317/401 (79.05%), Query Frame = 0

Query: 56  LRGKLNKVVLAYSGGLDTSVIVPWLRENYG-CEVVCFTADVGQGVKELDGLEQKARASGA 115
           +  K+NKVVLAYSGGLDTS+IVPWL++NYG  EV+C+ A++GQ   EL GLE+KA A+GA
Sbjct: 1   MSAKVNKVVLAYSGGLDTSIIVPWLKQNYGNPEVICYCANIGQD-DELSGLEEKAIATGA 60

Query: 116 CQLVVKDLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSH 175
            +  V+DL+EEFVRDF+FP L++GA+YER YLLGTS+ARP+IA+   ++A + GADA++H
Sbjct: 61  SKCYVEDLREEFVRDFLFPLLQSGAVYERTYLLGTSVARPLIARRQAEIALQEGADALAH 120

Query: 176 GCTGKGNDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYS 235
           GCTGKGNDQVRFELT+ A  P L V+APWREW+I  REDA++YA +HNVPV  T KSIYS
Sbjct: 121 GCTGKGNDQVRFELTYMAFAPHLKVIAPWREWNIRSREDALDYAAEHNVPVTATLKSIYS 180

Query: 236 RDRNLWHLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEML 295
           RDRN+WH+SHEG +LEDP NEP++ MY ++TDPE AP+EPEYV IG  QG PVSVNG+ L
Sbjct: 181 RDRNIWHMSHEGGILEDPWNEPEEAMYTLTTDPEAAPDEPEYVVIGFEQGTPVSVNGKRL 240

Query: 296 SPASLLTKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRE 355
            P  LL  LN++G KHGIGR+D+VENRLVGMKS GVYETPGGTIL  A + LE LTLDR+
Sbjct: 241 GPVELLLTLNDIGAKHGIGRVDLVENRLVGMKSHGVYETPGGTILRVAHQGLEQLTLDRD 300

Query: 356 TIQVKDSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRS 415
           T+  KD +A +YAELVY G+W+ PLRE++DAF+       TG   LKLYKG+ ++  RR+
Sbjct: 301 TLHYKDVIAHRYAELVYYGQWYTPLREALDAFVRVTQRNVTGEARLKLYKGNATLVGRRA 360

Query: 416 IYSLYREDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQ 456
             SLY  DI+SF     Y+Q DA GFI+++GLP +V+A+L+
Sbjct: 361 AKSLYNPDIASFTMSDSYNQKDAEGFIKIFGLPVKVQALLE 400

BLAST of MS019939 vs. ExPASy Swiss-Prot
Match: B8FWX2 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) OX=272564 GN=argG PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.0e-146
Identity = 246/397 (61.96%), Postives = 311/397 (78.34%), Query Frame = 0

Query: 60  LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVV 119
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +EL+ L +KA  SGA +L +
Sbjct: 1   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 60

Query: 120 KDLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 179
           +DL+EEFV DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 61  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 120

Query: 180 GNDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNL 239
           GNDQVRFEL   ALNP L ++APWR WDI  REDAI+YA +  +PVPVTK   YS DRN+
Sbjct: 121 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 180

Query: 240 WHLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASL 299
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+   QG+PVS+NGE L P  L
Sbjct: 181 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 240

Query: 300 LTKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVK 359
           L  LNE+GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+ A + LE LTLDR T+  K
Sbjct: 241 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 300

Query: 360 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLY 419
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+ S+   +S YSLY
Sbjct: 301 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 360

Query: 420 REDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
            E+ ++F    +Y+Q DA GFI L+GLP +VRA++++
Sbjct: 361 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 396

BLAST of MS019939 vs. ExPASy Swiss-Prot
Match: Q24ZG8 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=argG PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.0e-146
Identity = 246/397 (61.96%), Postives = 311/397 (78.34%), Query Frame = 0

Query: 60  LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVV 119
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +EL+ L +KA  SGA +L +
Sbjct: 9   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 68

Query: 120 KDLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 179
           +DL+EEFV DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 69  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 128

Query: 180 GNDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNL 239
           GNDQVRFEL   ALNP L ++APWR WDI  REDAI+YA +  +PVPVTK   YS DRN+
Sbjct: 129 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 188

Query: 240 WHLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASL 299
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+   QG+PVS+NGE L P  L
Sbjct: 189 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 248

Query: 300 LTKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVK 359
           L  LNE+GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+ A + LE LTLDR T+  K
Sbjct: 249 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 308

Query: 360 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLY 419
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+ S+   +S YSLY
Sbjct: 309 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 368

Query: 420 REDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
            E+ ++F    +Y+Q DA GFI L+GLP +VRA++++
Sbjct: 369 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 404

BLAST of MS019939 vs. ExPASy TrEMBL
Match: A0A6J1C7B7 (Citrulline--aspartate ligase OS=Momordica charantia OX=3673 GN=LOC111008910 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.8e-260
Identity = 453/458 (98.91%), Postives = 455/458 (99.34%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL
Sbjct: 37  LHSFQELGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFELTFFALNPKLNVVAPWR WDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELTFFALNPKLNVVAPWRGWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDP NEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL
Sbjct: 277 HLSHEGDVLEDPGNEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV
Sbjct: 457 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 494

BLAST of MS019939 vs. ExPASy TrEMBL
Match: A0A6J1E7A9 (Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 9.1e-246
Identity = 422/458 (92.14%), Postives = 443/458 (96.72%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAK  EIHGNS+A NRAS++RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 37  LHSFQELGAKASEIHGNSIACNRASVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGV EL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVMELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFVRD+IFPCLRAGA+YERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVRDYIFPCLRAGAVYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGD+LEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIV GLPVS+NGE+LSPASLL
Sbjct: 277 HLSHEGDILEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
           TK+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 TKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQGV 459
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML + +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHKDI 494

BLAST of MS019939 vs. ExPASy TrEMBL
Match: A0A0A0LWM9 (Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.9e-244
Identity = 420/456 (92.11%), Postives = 439/456 (96.27%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGN L+SN A ++RSNKK VIQAILQS+REIETTNG+GT GLRG+L
Sbjct: 37  LHSFQELGAKTCEIHGNGLSSNSAGVLRSNKKRVIQAILQSEREIETTNGIGTGGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQG+KEL+GLE KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGMKELEGLENKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDPANEPKKDMYM+S DPEDAPN+PEYVEIGIV GLPVSVNGE+LSPASLL
Sbjct: 277 HLSHEGDVLEDPANEPKKDMYMMSADPEDAPNKPEYVEIGIVGGLPVSVNGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
            K+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAA RELESLTLDRETIQVKD
Sbjct: 337 AKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAARELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS+YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 492

BLAST of MS019939 vs. ExPASy TrEMBL
Match: A0A5D3BHJ5 (Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004610 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.8e-244
Identity = 422/456 (92.54%), Postives = 439/456 (96.27%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGN L+SN + + RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 37  LHSFQELGAKTCEIHGNGLSSNSSGVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKEL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDPANEPKKDMYM+S DPEDAPNEPEYVEIGIV GLPVSVNGE+LSPASLL
Sbjct: 277 HLSHEGDVLEDPANEPKKDMYMMSADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
            K+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 AKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 492

BLAST of MS019939 vs. ExPASy TrEMBL
Match: A0A1S3AZP1 (Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.8e-244
Identity = 422/456 (92.54%), Postives = 439/456 (96.27%), Query Frame = 0

Query: 1   LHSWLQLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKL 60
           LHS+ +LGAKTCEIHGN L+SN + + RSNKK VIQAILQS+REIETTNG+ TRGLRG+L
Sbjct: 37  LHSFQELGAKTCEIHGNGLSSNSSGVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQL 96

Query: 61  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVK 120
           NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKEL+GLE+KARASGACQLVVK
Sbjct: 97  NKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVK 156

Query: 121 DLKEEFVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 180
           DLKEEFV DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG
Sbjct: 157 DLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKG 216

Query: 181 NDQVRFELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLW 240
           NDQVRFEL FFALNP LNVVAPWREWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLW
Sbjct: 217 NDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLW 276

Query: 241 HLSHEGDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLL 300
           HLSHEGDVLEDPANEPKKDMYM+S DPEDAPNEPEYVEIGIV GLPVSVNGE+LSPASLL
Sbjct: 277 HLSHEGDVLEDPANEPKKDMYMMSADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLL 336

Query: 301 TKLNELGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 360
            K+NE+GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD
Sbjct: 337 AKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKD 396

Query: 361 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYR 420
           SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V SRRS YSLYR
Sbjct: 397 SLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYR 456

Query: 421 EDISSFENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           EDISSFENGQIY+QADAAGFIRLYGLPTRVRAML +
Sbjct: 457 EDISSFENGQIYNQADAAGFIRLYGLPTRVRAMLHK 492

BLAST of MS019939 vs. TAIR 10
Match: AT4G24830.1 (arginosuccinate synthase family )

HSP 1 Score: 740.0 bits (1909), Expect = 1.2e-213
Identity = 364/451 (80.71%), Postives = 406/451 (90.02%), Query Frame = 0

Query: 6   QLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKLNKVVL 65
           +L  K  ++ GN++ +   +  RS K   I+A+L  D    TT+     GLRGKL KVVL
Sbjct: 43  ELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVL 102

Query: 66  AYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVKDLKEE 125
           AYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KEL+GLEQKA+ASGA QLVVKDL EE
Sbjct: 103 AYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEE 162

Query: 126 FVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVR 185
           FV+DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVR
Sbjct: 163 FVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVR 222

Query: 186 FELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 245
           FELTFF+LNP+L VVAPWREW+I GREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE
Sbjct: 223 FELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 282

Query: 246 GDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLLTKLNE 305
           GD+LEDPANEPKKDMYM+S DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LL +LN 
Sbjct: 283 GDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNT 342

Query: 306 LGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALK 365
           +GGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAA +ELESLTLDRE+IQVKD+LALK
Sbjct: 343 IGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLALK 402

Query: 366 YAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYREDISS 425
           YAE+VYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVT R+S  SLYR+DISS
Sbjct: 403 YAEMVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISS 462

Query: 426 FENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           FE  +IY+QADAAGFIRLYGLP ++RAML++
Sbjct: 463 FEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of MS019939 vs. TAIR 10
Match: AT4G24830.2 (arginosuccinate synthase family )

HSP 1 Score: 642.1 bits (1655), Expect = 3.4e-184
Identity = 325/451 (72.06%), Postives = 363/451 (80.49%), Query Frame = 0

Query: 6   QLGAKTCEIHGNSLASNRASIVRSNKKGVIQAILQSDREIETTNGVGTRGLRGKLNKVVL 65
           +L  K  ++ GN++ +   +  RS K   I+A+L  D    TT+     GLRGKL KVVL
Sbjct: 43  ELSLKRSQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVL 102

Query: 66  AYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELDGLEQKARASGACQLVVKDLKEE 125
           AYSGGLDTSVIVPWL+ENYGCEVVCFTADVGQG+KEL+GLEQKA+ASGA QLVVKDL EE
Sbjct: 103 AYSGGLDTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEE 162

Query: 126 FVRDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVR 185
           FV+DFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVR
Sbjct: 163 FVKDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVR 222

Query: 186 FELTFFALNPKLNVVAPWREWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 245
           FELTFF+LNP+L VVAPWREW+I GREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE
Sbjct: 223 FELTFFSLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHE 282

Query: 246 GDVLEDPANEPKKDMYMISTDPEDAPNEPEYVEIGIVQGLPVSVNGEMLSPASLLTKLNE 305
           GD+LEDPANEPKKDMYM+S DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LL +LN 
Sbjct: 283 GDLLEDPANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNT 342

Query: 306 LGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALK 365
           +GGKHGIGRIDMVENRLVGMKSR                                     
Sbjct: 343 IGGKHGIGRIDMVENRLVGMKSR------------------------------------- 402

Query: 366 YAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTSRRSIYSLYREDISS 425
                  GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVT R+S  SLYR+DISS
Sbjct: 403 -------GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISS 448

Query: 426 FENGQIYDQADAAGFIRLYGLPTRVRAMLQQ 457
           FE  +IY+QADAAGFIRLYGLP ++RAML++
Sbjct: 463 FEGSEIYNQADAAGFIRLYGLPMKIRAMLKK 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137474.17.8e-26098.91argininosuccinate synthase, chloroplastic-like [Momordica charantia][more]
XP_038895641.16.5e-24692.54argininosuccinate synthase, chloroplastic-like [Benincasa hispida][more]
XP_023519539.11.1e-24592.58argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6584145.11.4e-24592.36Argininosuccinate synthase, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
KAG7019736.11.4e-24592.36Argininosuccinate synthase, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
Q9SZX31.7e-21280.71Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q3A9W54.3e-15265.32Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA... [more]
A5V0J93.4e-14962.09Argininosuccinate synthase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=argG PE... [more]
B8FWX26.0e-14661.96Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / D... [more]
Q24ZG86.0e-14661.96Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=13811... [more]
Match NameE-valueIdentityDescription
A0A6J1C7B73.8e-26098.91Citrulline--aspartate ligase OS=Momordica charantia OX=3673 GN=LOC111008910 PE=3... [more]
A0A6J1E7A99.1e-24692.14Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 ... [more]
A0A0A0LWM92.9e-24492.11Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=... [more]
A0A5D3BHJ53.8e-24492.54Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AZP13.8e-24492.54Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24830.11.2e-21380.71arginosuccinate synthase family [more]
AT4G24830.23.4e-18472.06arginosuccinate synthase family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 62..231
e-value: 8.6E-165
score: 549.9
NoneNo IPR availableGENE3D1.20.5.470Single helix bincoord: 432..458
e-value: 1.7E-9
score: 38.9
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 61..225
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodyGENE3D3.90.1260.10Argininosuccinate synthetase, chain A, domain 2coord: 122..412
e-value: 8.6E-165
score: 549.9
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodySUPERFAMILY69864Argininosuccinate synthetase, C-terminal domaincoord: 230..456
IPR001518Argininosuccinate synthasePFAMPF00764Arginosuc_synthcoord: 64..450
e-value: 5.3E-160
score: 532.9
IPR001518Argininosuccinate synthaseTIGRFAMTIGR00032TIGR00032coord: 62..452
e-value: 6.8E-151
score: 501.0
IPR001518Argininosuccinate synthasePANTHERPTHR11587ARGININOSUCCINATE SYNTHASEcoord: 59..457
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00564ARGININOSUCCIN_SYN_1coord: 66..74
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00565ARGININOSUCCIN_SYN_2coord: 175..186
IPR023434Argininosuccinate synthase, type 1 subfamilyHAMAPMF_00005Arg_succ_synth_type1coord: 60..456
score: 53.918468
IPR023434Argininosuccinate synthase, type 1 subfamilyCDDcd01999Argininosuccinate_Synthasecoord: 63..447
e-value: 0.0
score: 663.077

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019939.1MS019939.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
molecular_function GO:0004055 argininosuccinate synthase activity
molecular_function GO:0005524 ATP binding