MS019895 (gene) Bitter gourd (TR) v1

BLAST of MS019895 vs. NCBI nr
Match: XP_022137167.1 (GDSL esterase/lipase At1g71250 [Momordica charantia])

HSP 1 Score: 751.9 bits (1940), Expect = 2.6e-213
Identity = 369/373 (98.93%), Postives = 370/373 (99.20%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MITQRRR+ AAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS
Sbjct: 10  MITQRRRFGAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 69

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDML IPNAPEFANPDTTGDKILHGVNYA
Sbjct: 70  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLRIPNAPEFANPDTTGDKILHGVNYA 129

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND
Sbjct: 130 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 189

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMPTLYNSRLRYTAPQFANLLLN YARQLLALQSMGLRKFVVAGVGPLGCIPNQR
Sbjct: 190 YINNYLMPTLYNSRLRYTAPQFANLLLNHYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 249

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY
Sbjct: 250 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 309

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS
Sbjct: 310 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 369

Query: 361 DSYPINIQQLALI 374
           DSYPINIQQLALI
Sbjct: 370 DSYPINIQQLALI 382

BLAST of MS019895 vs. NCBI nr
Match: XP_038876998.1 (GDSL esterase/lipase At1g71250 [Benincasa hispida])

HSP 1 Score: 637.5 bits (1643), Expect = 7.2e-179
Identity = 307/373 (82.31%), Postives = 345/373 (92.49%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MIT++ R+ A A+ L++VL+ +KCS+EMV AE    Y VS+VFVFGDSLVDVGNNNFLHS
Sbjct: 1   MITEQCRF-AMATVLIVVLIFLKCSKEMVVAE---NYRVSAVFVFGDSLVDVGNNNFLHS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARAN+YPYG+DY  GPTGRFSNG+TVIDMFVDMLGIPNAPEFANPDT+GD+I +GVNYA
Sbjct: 61  AARANYYPYGIDYDGGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRIYNGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLS+QVVNFE+T+++LRR+M S NLT YL KSIAFLAFGSND
Sbjct: 121 SAAAGILDETGRHYGDRYSLSEQVVNFESTLNELRRSMGSWNLTRYLSKSIAFLAFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+P LY SR RYT+ QFANLLLNRY RQLLALQS+GLRKFVVAGVGPLGCIPNQR
Sbjct: 181 YINNYLLPNLYTSRFRYTSKQFANLLLNRYTRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATG+ +PGRCVD+VN+MLG FNEGLRSLV+QLN+Q+PDSTFVYTN+YGIFGDILNNP+TY
Sbjct: 241 ATGVAIPGRCVDSVNEMLGAFNEGLRSLVIQLNSQHPDSTFVYTNIYGIFGDILNNPETY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCLNR E+VFWDAFHPTEAASA+LAGRAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLNRKEYVFWDAFHPTEAASAILAGRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPIN+QQLALI
Sbjct: 361 DSYPINVQQLALI 369

BLAST of MS019895 vs. NCBI nr
Match: XP_008440329.1 (PREDICTED: GDSL esterase/lipase At1g71250 isoform X1 [Cucumis melo] >KAA0055368.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYJ99295.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 626.3 bits (1614), Expect = 1.7e-175
Identity = 303/373 (81.23%), Postives = 337/373 (90.35%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MI+ R     AA+  V++LV +KCS +MV+AE    YGV ++FVFGDSLVDVGNNNFLHS
Sbjct: 1   MISDRLHGFVAAAAGVVLLVFLKCSNQMVAAE---NYGVPAIFVFGDSLVDVGNNNFLHS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARAN+YPYG+D+  GPTGRFSNG+TVIDMFVDMLGIPNAPEFANPDT+GD+IL+GVNYA
Sbjct: 61  AARANYYPYGIDFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLSQQVVNFE+T++DLRR+M S NLT YL KSIAFLAFGSND
Sbjct: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMP LY +R RY + QFANLLLNRY+RQLLAL+S+GLRK VVAGVGPLGCIPNQR
Sbjct: 181 YINNYLMPNLYTTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATGL +PGRC DNVN+MLG FNEGL+SLV QLN+Q PDS FVYTN+YGIFGDILNNP+TY
Sbjct: 241 ATGLTIPGRCADNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCLNRNE+VFWDAFHPTEAAS VLAGRAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPIN+QQLALI
Sbjct: 361 DSYPINVQQLALI 370

BLAST of MS019895 vs. NCBI nr
Match: XP_023542703.1 (GDSL esterase/lipase At1g71250-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.7 bits (1576), Expect = 4.3e-171
Identity = 297/373 (79.62%), Postives = 329/373 (88.20%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           M  +R   A A +TLVLVLVL +CS++M  AE     GV ++FVFGDSLVDVGNNNFLHS
Sbjct: 1   MTKRRPATATATATLVLVLVLFECSKQMALAE----NGVPAIFVFGDSLVDVGNNNFLHS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARANFYPYG+D+  GPTGRFSNG+T+IDM VD LGIPNA EFANPDT+GD+IL GVNYA
Sbjct: 61  AARANFYPYGIDFRGGPTGRFSNGRTIIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYGERYSL+QQVVNFE+T +DLR++M + NLT YL +SIAF+AFGSND
Sbjct: 121 SAAAGILDETGRHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSIAFMAFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMP LY SR RYT+ QFAN LLN Y RQLLAL+S GLRKFVVAG+GPLGCIPNQR
Sbjct: 181 YINNYLMPNLYTSRFRYTSNQFANHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATG+ LPGRCVDNVN+MLGDFNEGLRSLV QLN+Q PDS FVYTN+YGIFGDILNNPDTY
Sbjct: 241 ATGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNNPDTY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCLNR+E+ FWDA+HPTEAAS VLA RAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLNRDEYAFWDAYHPTEAASVVLADRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPINIQQLAL+
Sbjct: 361 DSYPINIQQLALL 369

BLAST of MS019895 vs. NCBI nr
Match: XP_023519631.1 (GDSL esterase/lipase At1g71250-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.1 bits (1572), Expect = 1.2e-170
Identity = 297/373 (79.62%), Postives = 332/373 (89.01%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MI Q  R AA+A+ L+L++V ++CS+E VS       GV ++FVFGDSLVDVGNNNFL S
Sbjct: 1   MIIQPPRLAASAALLLLLVVFLRCSDETVSE----TNGVPAIFVFGDSLVDVGNNNFLRS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
            ARAN+YPYG+D+ +GPTGRFSNG+TVIDMFVDM GIPNAPEFANP+TTG  ILHGVNYA
Sbjct: 61  VARANYYPYGIDFLSGPTGRFSNGRTVIDMFVDMFGIPNAPEFANPNTTGVGILHGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYGERYSLSQQVVNFE+T+++LRR+M S NL+ YL KS+AFL FGSND
Sbjct: 121 SAAAGILDETGRHYGERYSLSQQVVNFESTLNELRRSMGSWNLSRYLSKSLAFLVFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYL+P LY SR RYT  QFANLLLNRY+RQLLALQS+GLRKFVVAGVGPLGCIPNQR
Sbjct: 181 YINNYLLPELYTSRFRYTPNQFANLLLNRYSRQLLALQSVGLRKFVVAGVGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           A+G   PGRCVDNVN+MLG FNEGLRSLV QLN+Q+P+STFVY NVY IFGDILNNPDTY
Sbjct: 241 ASGAATPGRCVDNVNKMLGAFNEGLRSLVTQLNSQHPESTFVYVNVYAIFGDILNNPDTY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCL RNE+VFWDAFHPTEAASA+LAGRAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLYRNEYVFWDAFHPTEAASAILAGRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPIN++QLALI
Sbjct: 361 DSYPINVRQLALI 369

BLAST of MS019895 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.6e-128
Identity = 224/377 (59.42%), Postives = 282/377 (74.80%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVL----MKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNN 60
           M T R++        VL+L L    +    E+V+ + R    V ++FV GDSLVD GNNN
Sbjct: 1   MNTNRKKMKVHIGGYVLILALTVSVILQQPELVTGQAR----VPAMFVLGDSLVDAGNNN 60

Query: 61  FLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHG 120
           FL + ARANF PYG+D +  PTGRFSNG T ID+   +L IP+ P FA+P T+G++IL G
Sbjct: 61  FLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQG 120

Query: 121 VNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAF 180
           VNYASAAAGILD +G +YG R+SL+QQ+VN E T+S LR  MS  N T+YL +S+  L F
Sbjct: 121 VNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVF 180

Query: 181 GSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCI 240
           GSNDYINNYLMP LY+S +R+  P FANLLL++YARQLL L S+GLRK  + GV PLGCI
Sbjct: 181 GSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCI 240

Query: 241 PNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNN 300
           PNQRA G+  P RCVD+VNQ+LG FN+GL+SLV QLN ++P + +VY N Y   GDILNN
Sbjct: 241 PNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNN 300

Query: 301 PDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFR 360
           P  YGF VVD ACCG+G+N+G ITCLPLQ PC NRN++VFWDAFHPT+ A+++LA RAF 
Sbjct: 301 PAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFY 360

Query: 361 GPPSDSYPINIQQLALI 374
           GPPSD+YP+N+QQ+ L+
Sbjct: 361 GPPSDAYPVNVQQMTLL 373

BLAST of MS019895 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.0e-103
Identity = 184/335 (54.93%), Postives = 233/335 (69.55%), Query Frame = 0

Query: 41  SVFVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAG-PTGRFSNGQTVIDMFVDMLGIPN 100
           ++FVFGDSLVD GNNN L+S AR+N+ PYG+D+    PTGRFSNG+T++D   ++LG+P 
Sbjct: 49  AMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGELLGLPE 108

Query: 101 APEFANPDTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMS 160
            P F +    G  ILHGVNYASAA GIL+ETGRH GER+S+ +QV NFE T+ ++ R+M 
Sbjct: 109 IPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSMR 168

Query: 161 SGNLTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 220
             ++  Y+ KS+  ++ G+NDYINNYL P L+ S   Y    FA+LLL+ +   LL L  
Sbjct: 169 KESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELYG 228

Query: 221 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQN--- 280
            G RKFV+AGVGPLGCIP+Q A    LPG CV+ VN+M   FN  L SLV +LN+ N   
Sbjct: 229 KGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSDNKTA 288

Query: 281 PDSTFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVF 340
            ++ FVY N YG   DIL NP  YGF V D  CCGVG+NRG ITCLPL  PC  R+ HVF
Sbjct: 289 SEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVF 348

Query: 341 WDAFHPTEAASAVLAGRAFRGPPSDSYPINIQQLA 372
           WDAFHPT+A + ++A RAF G  SD YPIN+ QL+
Sbjct: 349 WDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLS 383

BLAST of MS019895 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.2e-91
Identity = 181/359 (50.42%), Postives = 243/359 (67.69%), Query Frame = 0

Query: 17  LVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAG 76
           +VLVL+     +V A+ + +  V   FVFGDSLVD GNNN L S AR+N++PYG+D+  G
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQ--VPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GG 69

Query: 77  PTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYASAAAGILDETGRHYGE 136
           PTGRFSNG+T +D+  ++LG  N    A    +G +IL GVNYASAAAGI +ETGR  G+
Sbjct: 70  PTGRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQ 129

Query: 137 RYSLSQQVVNFENTVSDLRRTM-SSGNLTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRL 196
           R S S QV N++ TVS + + +       +YL + I  +  GSNDY+NNY MPT Y+S  
Sbjct: 130 RISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSR 189

Query: 197 RYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQRATGLGLP-GR-CVDN 256
           ++T  Q+AN L++RY+ QL AL + G RKF ++G+G +GC PN  A   G P GR CVD 
Sbjct: 190 QFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA---GSPDGRTCVDR 249

Query: 257 VNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVG 316
           +N     FN  LRSLV QLN  +PD+ F+Y N YGIF D++ NP  +GFRV +  CCG+G
Sbjct: 250 INSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIG 309

Query: 317 QNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRG-PPSDSYPINIQQLA 372
           +N G ITCLP Q PC +RN +VFWDAFHPTEAA+ ++A R++     SD+YP++I +LA
Sbjct: 310 RNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLA 361

BLAST of MS019895 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.5e-90
Identity = 171/333 (51.35%), Postives = 214/333 (64.26%), Query Frame = 0

Query: 43  FVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPE 102
           F+FGDSLVD GNNN L S ARAN++PYG+D+ AGPTGRFSNG T +D+   +LG     +
Sbjct: 31  FIFGDSLVDNGNNNQLQSLARANYFPYGIDFAAGPTGRFSNGLTTVDVIAQLLGF---ED 90

Query: 103 FANP--DTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSS 162
           +  P     G  IL GVNYASAAAGI DETGR  G R + + QV N  NTVS +   +  
Sbjct: 91  YITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGD 150

Query: 163 GN-LTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 222
            N  +NYL K I  +  GSNDY+NNY MPT Y++  +++   +A+ L+ RY  QL  L +
Sbjct: 151 QNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYT 210

Query: 223 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDS 282
            G RKF + GVG +GC PN+ A        C + +N     FN  L S+V   N   PD+
Sbjct: 211 NGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDA 270

Query: 283 TFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDA 342
            F Y N YGIF DI+ NP  YGFRV +  CCGVG+N G ITCLP Q PCLNRNE+VFWDA
Sbjct: 271 KFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDA 330

Query: 343 FHPTEAASAVLAGRAF-RGPPSDSYPINIQQLA 372
           FHP EAA+ V+  R+F R   SD++P +IQQLA
Sbjct: 331 FHPGEAANIVIGRRSFKREAASDAHPYDIQQLA 360

BLAST of MS019895 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-89
Identity = 167/333 (50.15%), Postives = 215/333 (64.56%), Query Frame = 0

Query: 43  FVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPE 102
           F+FGDSLVD GNNN L S ARAN++PYG+D+  GPTGRFSNG+T +D+  ++LG     +
Sbjct: 30  FIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVITELLGF---DD 89

Query: 103 FANP--DTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSS 162
           +  P  +  G+ IL GVNYASAAAGI +ETGR  G R + + QV N  NTVS +   +  
Sbjct: 90  YITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQVVNILGD 149

Query: 163 GN-LTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 222
            N   NYL K I  +  GSNDY+NNY MP  Y++  +Y+   +AN L+NRY  QL  + +
Sbjct: 150 ENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQLRIMYN 209

Query: 223 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDS 282
            G RKF + G+G +GC PN+ A        C + +N     FN  L SLV   N   P +
Sbjct: 210 NGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFNQNTPGA 269

Query: 283 TFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDA 342
            F Y N YGIF D++ NP  YGFRV +  CCGVG+N G ITCLP Q PCLNR+E+VFWDA
Sbjct: 270 KFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDEYVFWDA 329

Query: 343 FHPTEAASAVLAGRAF-RGPPSDSYPINIQQLA 372
           FHP EAA+ V+  R+F R   SD++P +IQQLA
Sbjct: 330 FHPGEAANVVIGSRSFQRESASDAHPYDIQQLA 359

BLAST of MS019895 vs. ExPASy TrEMBL
Match: A0A6J1C7I1 (GDSL esterase/lipase At1g71250 OS=Momordica charantia OX=3673 GN=LOC111008710 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.3e-213
Identity = 369/373 (98.93%), Postives = 370/373 (99.20%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MITQRRR+ AAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS
Sbjct: 10  MITQRRRFGAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 69

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDML IPNAPEFANPDTTGDKILHGVNYA
Sbjct: 70  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLRIPNAPEFANPDTTGDKILHGVNYA 129

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND
Sbjct: 130 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 189

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMPTLYNSRLRYTAPQFANLLLN YARQLLALQSMGLRKFVVAGVGPLGCIPNQR
Sbjct: 190 YINNYLMPTLYNSRLRYTAPQFANLLLNHYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 249

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY
Sbjct: 250 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 309

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS
Sbjct: 310 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 369

Query: 361 DSYPINIQQLALI 374
           DSYPINIQQLALI
Sbjct: 370 DSYPINIQQLALI 382

BLAST of MS019895 vs. ExPASy TrEMBL
Match: A0A5A7UK29 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005080 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 8.1e-176
Identity = 303/373 (81.23%), Postives = 337/373 (90.35%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MI+ R     AA+  V++LV +KCS +MV+AE    YGV ++FVFGDSLVDVGNNNFLHS
Sbjct: 1   MISDRLHGFVAAAAGVVLLVFLKCSNQMVAAE---NYGVPAIFVFGDSLVDVGNNNFLHS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARAN+YPYG+D+  GPTGRFSNG+TVIDMFVDMLGIPNAPEFANPDT+GD+IL+GVNYA
Sbjct: 61  AARANYYPYGIDFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLSQQVVNFE+T++DLRR+M S NLT YL KSIAFLAFGSND
Sbjct: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMP LY +R RY + QFANLLLNRY+RQLLAL+S+GLRK VVAGVGPLGCIPNQR
Sbjct: 181 YINNYLMPNLYTTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATGL +PGRC DNVN+MLG FNEGL+SLV QLN+Q PDS FVYTN+YGIFGDILNNP+TY
Sbjct: 241 ATGLTIPGRCADNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCLNRNE+VFWDAFHPTEAAS VLAGRAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPIN+QQLALI
Sbjct: 361 DSYPINVQQLALI 370

BLAST of MS019895 vs. ExPASy TrEMBL
Match: A0A1S3B1L6 (GDSL esterase/lipase At1g71250 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484808 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 8.1e-176
Identity = 303/373 (81.23%), Postives = 337/373 (90.35%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHS 60
           MI+ R     AA+  V++LV +KCS +MV+AE    YGV ++FVFGDSLVDVGNNNFLHS
Sbjct: 1   MISDRLHGFVAAAAGVVLLVFLKCSNQMVAAE---NYGVPAIFVFGDSLVDVGNNNFLHS 60

Query: 61  AARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYA 120
           AARAN+YPYG+D+  GPTGRFSNG+TVIDMFVDMLGIPNAPEFANPDT+GD+IL+GVNYA
Sbjct: 61  AARANYYPYGIDFSDGPTGRFSNGRTVIDMFVDMLGIPNAPEFANPDTSGDRILNGVNYA 120

Query: 121 SAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSND 180
           SAAAGILDETGRHYG+RYSLSQQVVNFE+T++DLRR+M S NLT YL KSIAFLAFGSND
Sbjct: 121 SAAAGILDETGRHYGDRYSLSQQVVNFESTLNDLRRSMGSWNLTRYLSKSIAFLAFGSND 180

Query: 181 YINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQR 240
           YINNYLMP LY +R RY + QFANLLLNRY+RQLLAL+S+GLRK VVAGVGPLGCIPNQR
Sbjct: 181 YINNYLMPNLYTTRFRYNSNQFANLLLNRYSRQLLALRSVGLRKLVVAGVGPLGCIPNQR 240

Query: 241 ATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTY 300
           ATGL +PGRC DNVN+MLG FNEGL+SLV QLN+Q PDS FVYTN+YGIFGDILNNP+TY
Sbjct: 241 ATGLTIPGRCADNVNEMLGAFNEGLKSLVTQLNSQYPDSNFVYTNIYGIFGDILNNPETY 300

Query: 301 GFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPS 360
           GFRVVDTACCGVG NRG ITCLPLQFPCLNRNE+VFWDAFHPTEAAS VLAGRAFRGPPS
Sbjct: 301 GFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYVFWDAFHPTEAASNVLAGRAFRGPPS 360

Query: 361 DSYPINIQQLALI 374
           DSYPIN+QQLALI
Sbjct: 361 DSYPINVQQLALI 370

BLAST of MS019895 vs. ExPASy TrEMBL
Match: A0A1S4DTZ1 (GDSL esterase/lipase At1g71250 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484808 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 3.0e-170
Identity = 288/337 (85.46%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 37  YGVSSVFVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLG 96
           YGV ++FVFGDSLVDVGNNNFLHSAARAN+YPYG+D+  GPTGRFSNG+TVIDMFVDMLG
Sbjct: 12  YGVPAIFVFGDSLVDVGNNNFLHSAARANYYPYGIDFSDGPTGRFSNGRTVIDMFVDMLG 71

Query: 97  IPNAPEFANPDTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRR 156
           IPNAPEFANPDT+GD+IL+GVNYASAAAGILDETGRHYG+RYSLSQQVVNFE+T++DLRR
Sbjct: 72  IPNAPEFANPDTSGDRILNGVNYASAAAGILDETGRHYGDRYSLSQQVVNFESTLNDLRR 131

Query: 157 TMSSGNLTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLA 216
           +M S NLT YL KSIAFLAFGSNDYINNYLMP LY +R RY + QFANLLLNRY+RQLLA
Sbjct: 132 SMGSWNLTRYLSKSIAFLAFGSNDYINNYLMPNLYTTRFRYNSNQFANLLLNRYSRQLLA 191

Query: 217 LQSMGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQN 276
           L+S+GLRK VVAGVGPLGCIPNQRATGL +PGRC DNVN+MLG FNEGL+SLV QLN+Q 
Sbjct: 192 LRSVGLRKLVVAGVGPLGCIPNQRATGLTIPGRCADNVNEMLGAFNEGLKSLVTQLNSQY 251

Query: 277 PDSTFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVF 336
           PDS FVYTN+YGIFGDILNNP+TYGFRVVDTACCGVG NRG ITCLPLQFPCLNRNE+VF
Sbjct: 252 PDSNFVYTNIYGIFGDILNNPETYGFRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYVF 311

Query: 337 WDAFHPTEAASAVLAGRAFRGPPSDSYPINIQQLALI 374
           WDAFHPTEAAS VLAGRAFRGPPSDSYPIN+QQLALI
Sbjct: 312 WDAFHPTEAASNVLAGRAFRGPPSDSYPINVQQLALI 348

BLAST of MS019895 vs. ExPASy TrEMBL
Match: A0A6J1JZ26 (GDSL esterase/lipase At1g71250-like OS=Cucurbita maxima OX=3661 GN=LOC111490136 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.6e-170
Identity = 296/372 (79.57%), Postives = 329/372 (88.44%), Query Frame = 0

Query: 2   ITQRRRYAAAASTLVLVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHSA 61
           +T+RR    A +TLVLVLVL +CS++M  AE     GV ++FVFGDSLVDVGNNNFLHSA
Sbjct: 6   MTKRR---PATATLVLVLVLFECSKQMALAE----NGVPAIFVFGDSLVDVGNNNFLHSA 65

Query: 62  ARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYAS 121
           ARANFYPYG+D+  GPTGRFSNG+TVIDM VD LGIPNA EFANPDT+GD+IL GVNYAS
Sbjct: 66  ARANFYPYGIDFRGGPTGRFSNGRTVIDMIVDYLGIPNAQEFANPDTSGDRILKGVNYAS 125

Query: 122 AAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAFGSNDY 181
           AAAGILDETGRHYGERYSL+QQVVNFE+T +DLR++M + NLT YL +SI F+AFGSNDY
Sbjct: 126 AAAGILDETGRHYGERYSLNQQVVNFESTRNDLRKSMDNWNLTRYLSRSITFMAFGSNDY 185

Query: 182 INNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQRA 241
           INNYLMP LY SR RYT+ QFA+ LLN Y RQLLAL+S GLRKFVVAG+GPLGCIPNQRA
Sbjct: 186 INNYLMPNLYTSRFRYTSDQFADHLLNSYTRQLLALRSAGLRKFVVAGIGPLGCIPNQRA 245

Query: 242 TGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTYG 301
           TG+ LPGRCVDNVN+MLGDFNEGLRSLV QLN+Q PDS FVYTN+YGIFGDILN+PDTYG
Sbjct: 246 TGVALPGRCVDNVNEMLGDFNEGLRSLVTQLNSQYPDSYFVYTNIYGIFGDILNHPDTYG 305

Query: 302 FRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRGPPSD 361
           FRVVDTACCGVG NRG ITCLPLQFPCLNRNE+ FWDA+HPTEAAS VLA RAFRGPPSD
Sbjct: 306 FRVVDTACCGVGLNRGQITCLPLQFPCLNRNEYAFWDAYHPTEAASVVLADRAFRGPPSD 365

Query: 362 SYPINIQQLALI 374
           SYPINIQQLAL+
Sbjct: 366 SYPINIQQLALL 370

BLAST of MS019895 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.1e-129
Identity = 224/377 (59.42%), Postives = 282/377 (74.80%), Query Frame = 0

Query: 1   MITQRRRYAAAASTLVLVLVL----MKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNN 60
           M T R++        VL+L L    +    E+V+ + R    V ++FV GDSLVD GNNN
Sbjct: 1   MNTNRKKMKVHIGGYVLILALTVSVILQQPELVTGQAR----VPAMFVLGDSLVDAGNNN 60

Query: 61  FLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHG 120
           FL + ARANF PYG+D +  PTGRFSNG T ID+   +L IP+ P FA+P T+G++IL G
Sbjct: 61  FLQTVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEIPSPPPFADPTTSGNRILQG 120

Query: 121 VNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSSGNLTNYLCKSIAFLAF 180
           VNYASAAAGILD +G +YG R+SL+QQ+VN E T+S LR  MS  N T+YL +S+  L F
Sbjct: 121 VNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVF 180

Query: 181 GSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCI 240
           GSNDYINNYLMP LY+S +R+  P FANLLL++YARQLL L S+GLRK  + GV PLGCI
Sbjct: 181 GSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCI 240

Query: 241 PNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNN 300
           PNQRA G+  P RCVD+VNQ+LG FN+GL+SLV QLN ++P + +VY N Y   GDILNN
Sbjct: 241 PNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNN 300

Query: 301 PDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFR 360
           P  YGF VVD ACCG+G+N+G ITCLPLQ PC NRN++VFWDAFHPT+ A+++LA RAF 
Sbjct: 301 PAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFY 360

Query: 361 GPPSDSYPINIQQLALI 374
           GPPSD+YP+N+QQ+ L+
Sbjct: 361 GPPSDAYPVNVQQMTLL 373

BLAST of MS019895 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.1e-104
Identity = 184/335 (54.93%), Postives = 233/335 (69.55%), Query Frame = 0

Query: 41  SVFVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAG-PTGRFSNGQTVIDMFVDMLGIPN 100
           ++FVFGDSLVD GNNN L+S AR+N+ PYG+D+    PTGRFSNG+T++D   ++LG+P 
Sbjct: 49  AMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGELLGLPE 108

Query: 101 APEFANPDTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMS 160
            P F +    G  ILHGVNYASAA GIL+ETGRH GER+S+ +QV NFE T+ ++ R+M 
Sbjct: 109 IPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSMR 168

Query: 161 SGNLTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 220
             ++  Y+ KS+  ++ G+NDYINNYL P L+ S   Y    FA+LLL+ +   LL L  
Sbjct: 169 KESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELYG 228

Query: 221 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQN--- 280
            G RKFV+AGVGPLGCIP+Q A    LPG CV+ VN+M   FN  L SLV +LN+ N   
Sbjct: 229 KGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSDNKTA 288

Query: 281 PDSTFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVF 340
            ++ FVY N YG   DIL NP  YGF V D  CCGVG+NRG ITCLPL  PC  R+ HVF
Sbjct: 289 SEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVF 348

Query: 341 WDAFHPTEAASAVLAGRAFRGPPSDSYPINIQQLA 372
           WDAFHPT+A + ++A RAF G  SD YPIN+ QL+
Sbjct: 349 WDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLS 383

BLAST of MS019895 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 8.5e-93
Identity = 181/359 (50.42%), Postives = 243/359 (67.69%), Query Frame = 0

Query: 17  LVLVLMKCSEEMVSAEYRYRYGVSSVFVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAG 76
           +VLVL+     +V A+ + +  V   FVFGDSLVD GNNN L S AR+N++PYG+D+  G
Sbjct: 10  VVLVLLCFGFSVVKAQAQAQ--VPCFFVFGDSLVDNGNNNGLISIARSNYFPYGIDF-GG 69

Query: 77  PTGRFSNGQTVIDMFVDMLGIPNAPEFANPDTTGDKILHGVNYASAAAGILDETGRHYGE 136
           PTGRFSNG+T +D+  ++LG  N    A    +G +IL GVNYASAAAGI +ETGR  G+
Sbjct: 70  PTGRFSNGKTTVDVIAELLGF-NGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQLGQ 129

Query: 137 RYSLSQQVVNFENTVSDLRRTM-SSGNLTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRL 196
           R S S QV N++ TVS + + +       +YL + I  +  GSNDY+NNY MPT Y+S  
Sbjct: 130 RISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSR 189

Query: 197 RYTAPQFANLLLNRYARQLLALQSMGLRKFVVAGVGPLGCIPNQRATGLGLP-GR-CVDN 256
           ++T  Q+AN L++RY+ QL AL + G RKF ++G+G +GC PN  A   G P GR CVD 
Sbjct: 190 QFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALA---GSPDGRTCVDR 249

Query: 257 VNQMLGDFNEGLRSLVVQLNTQNPDSTFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVG 316
           +N     FN  LRSLV QLN  +PD+ F+Y N YGIF D++ NP  +GFRV +  CCG+G
Sbjct: 250 INSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIG 309

Query: 317 QNRGVITCLPLQFPCLNRNEHVFWDAFHPTEAASAVLAGRAFRG-PPSDSYPINIQQLA 372
           +N G ITCLP Q PC +RN +VFWDAFHPTEAA+ ++A R++     SD+YP++I +LA
Sbjct: 310 RNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRLA 361

BLAST of MS019895 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.6e-91
Identity = 171/333 (51.35%), Postives = 214/333 (64.26%), Query Frame = 0

Query: 43  FVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPE 102
           F+FGDSLVD GNNN L S ARAN++PYG+D+ AGPTGRFSNG T +D+   +LG     +
Sbjct: 31  FIFGDSLVDNGNNNQLQSLARANYFPYGIDFAAGPTGRFSNGLTTVDVIAQLLGF---ED 90

Query: 103 FANP--DTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSS 162
           +  P     G  IL GVNYASAAAGI DETGR  G R + + QV N  NTVS +   +  
Sbjct: 91  YITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVVNILGD 150

Query: 163 GN-LTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 222
            N  +NYL K I  +  GSNDY+NNY MPT Y++  +++   +A+ L+ RY  QL  L +
Sbjct: 151 QNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQLRVLYT 210

Query: 223 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDS 282
            G RKF + GVG +GC PN+ A        C + +N     FN  L S+V   N   PD+
Sbjct: 211 NGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQNTPDA 270

Query: 283 TFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDA 342
            F Y N YGIF DI+ NP  YGFRV +  CCGVG+N G ITCLP Q PCLNRNE+VFWDA
Sbjct: 271 KFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEYVFWDA 330

Query: 343 FHPTEAASAVLAGRAF-RGPPSDSYPINIQQLA 372
           FHP EAA+ V+  R+F R   SD++P +IQQLA
Sbjct: 331 FHPGEAANIVIGRRSFKREAASDAHPYDIQQLA 360

BLAST of MS019895 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.9e-91
Identity = 167/333 (50.15%), Postives = 215/333 (64.56%), Query Frame = 0

Query: 43  FVFGDSLVDVGNNNFLHSAARANFYPYGVDYHAGPTGRFSNGQTVIDMFVDMLGIPNAPE 102
           F+FGDSLVD GNNN L S ARAN++PYG+D+  GPTGRFSNG+T +D+  ++LG     +
Sbjct: 30  FIFGDSLVDSGNNNRLTSLARANYFPYGIDFQYGPTGRFSNGKTTVDVITELLGF---DD 89

Query: 103 FANP--DTTGDKILHGVNYASAAAGILDETGRHYGERYSLSQQVVNFENTVSDLRRTMSS 162
           +  P  +  G+ IL GVNYASAAAGI +ETGR  G R + + QV N  NTVS +   +  
Sbjct: 90  YITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVSQVVNILGD 149

Query: 163 GN-LTNYLCKSIAFLAFGSNDYINNYLMPTLYNSRLRYTAPQFANLLLNRYARQLLALQS 222
            N   NYL K I  +  GSNDY+NNY MP  Y++  +Y+   +AN L+NRY  QL  + +
Sbjct: 150 ENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYTEQLRIMYN 209

Query: 223 MGLRKFVVAGVGPLGCIPNQRATGLGLPGRCVDNVNQMLGDFNEGLRSLVVQLNTQNPDS 282
            G RKF + G+G +GC PN+ A        C + +N     FN  L SLV   N   P +
Sbjct: 210 NGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDHFNQNTPGA 269

Query: 283 TFVYTNVYGIFGDILNNPDTYGFRVVDTACCGVGQNRGVITCLPLQFPCLNRNEHVFWDA 342
            F Y N YGIF D++ NP  YGFRV +  CCGVG+N G ITCLP Q PCLNR+E+VFWDA
Sbjct: 270 KFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRDEYVFWDA 329

Query: 343 FHPTEAASAVLAGRAF-RGPPSDSYPINIQQLA 372
           FHP EAA+ V+  R+F R   SD++P +IQQLA
Sbjct: 330 FHPGEAANVVIGSRSFQRESASDAHPYDIQQLA 359