MS019512 (gene) Bitter gourd (TR) v1

BLAST of MS019512 vs. NCBI nr
Match: XP_022144773.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Momordica charantia])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 512/524 (97.71%), Postives = 515/524 (98.28%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS
Sbjct: 53  FHFQPRRHWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 112

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN
Sbjct: 113 PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 172

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL
Sbjct: 173 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 232

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD
Sbjct: 233 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 292

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ
Sbjct: 293 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 352

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF
Sbjct: 353 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 412

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH
Sbjct: 413 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 472

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGK 
Sbjct: 473 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKA 532

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN
Sbjct: 533 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 576

BLAST of MS019512 vs. NCBI nr
Match: XP_022938863.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita moschata])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 425/524 (81.11%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTG+DP NRQVQNYA+PANLSDPYL DW+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRS+DFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+GK+GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DD++KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+N LRG +V+M+++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDATSSSL+  GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGKT
Sbjct: 477 HN-HVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKT 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFG+AHL+VFNNGSE ITVD LSAWSM+SA +N
Sbjct: 537 CITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 579

BLAST of MS019512 vs. NCBI nr
Match: XP_023005784.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 925.2 bits (2390), Expect = 2.4e-265
Identity = 425/524 (81.11%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTGIDP NRQVQNYA+PANLSDPYL DW+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTDWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRSRDFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSDSGHWKTIIGSKRRKRGVAYLYRSRDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+G +GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGNQGLDVSVTGNLVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DDV+KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWNGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDVSKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+NRLRG +V+++++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNRLRGKQVKLSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L+EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLEEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDA+SSSL+ +GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGK 
Sbjct: 477 HN-HVVLLCSDASSSSLKKEGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKI 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFG+AHL+VFNNG+E ITVD LSAWSM SA VN
Sbjct: 537 CITSRVYPTKAVFGDAHLYVFNNGTEAITVDYLSAWSMTSATVN 579

BLAST of MS019512 vs. NCBI nr
Match: KAG6596582.1 (Beta-fructofuranosidase, insoluble isoenzyme 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 924.9 bits (2389), Expect = 3.2e-265
Identity = 425/524 (81.11%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPRRHWIN---GPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTG+DP NRQVQNYA+PANLSDPYL DW+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRS+DFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+GK+GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DD++KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+N LRG +V+M+++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDATSSSL+  GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGKT
Sbjct: 477 HN-HVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKT 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFG+AHL+VFNNGSE ITVD LSAWSM+SA +N
Sbjct: 537 CITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 576

BLAST of MS019512 vs. NCBI nr
Match: XP_023540070.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 923.7 bits (2386), Expect = 7.1e-265
Identity = 423/524 (80.73%), Postives = 472/524 (90.08%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPQRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTG+DP NRQVQNYA+PANLSDPYL +W+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTEWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRSRDFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSRDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+G +GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGNQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DD++KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+N LRG +V+++++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNGLRGKQVKLSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L+EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLEEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDATSSSL+  GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGKT
Sbjct: 477 HN-HVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKT 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVF +AHL+VFNNGSE ITVD LSAWSM+SA VN
Sbjct: 537 CITSRVYPTKAVFEDAHLYVFNNGSEAITVDYLSAWSMRSATVN 579

BLAST of MS019512 vs. ExPASy Swiss-Prot
Match: P26792 (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 PE=1 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.2e-228
Identity = 372/526 (70.72%), Postives = 438/526 (83.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           Y F PK  + N   GPMY+KG+YHLFYQYNPKGAVWGNIVWAHS S DLINW  L  A+ 
Sbjct: 63  YHFQPKQNWINDPNGPMYYKGVYHLFYQYNPKGAVWGNIVWAHSVSTDLINWTPLEPAIF 122

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGI---DPHNRQVQNYAVPANLSDPYLRDWVKP 120
           PSKPFD  GC SGSATILPG +PVI+YTGI    P N QVQNYA+PANLSDPYLR W+KP
Sbjct: 123 PSKPFDKYGCRSGSATILPGNKPVILYTGIVEGPPKNVQVQNYAIPANLSDPYLRKWIKP 182

Query: 121 DFNPVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAK 180
           D NP+V  ++G NA+AFRDPTT W  KSGHW+ ++GS+R  RG+AYLYRS+DF+KWT+AK
Sbjct: 183 DNNPLVVANNGENATAFRDPTTAWLDKSGHWKMLVGSKRNRRGIAYLYRSKDFIKWTKAK 242

Query: 181 HPLHSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLE 240
           HP+HS ANTGMWECPDF+PV L G  GLDTSV+G  VKHVLKVSLDLTRY+YYTVG YL 
Sbjct: 243 HPIHSQANTGMWECPDFFPVSLKGLNGLDTSVTGESVKHVLKVSLDLTRYEYYTVGTYLT 302

Query: 241 NKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWA 300
           +KDRY+PDNTS DGW GLRYDYGN+YASKTF+D  K RR+LWGWANESD+   DVAKGWA
Sbjct: 303 DKDRYIPDNTSVDGWAGLRYDYGNFYASKTFFDPSKNRRILWGWANESDSTAHDVAKGWA 362

Query: 301 GIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRK-LAKGQHVEIHGISAAQADV 360
           GIQ IPRT+WLDP+ KQL+QWP+EE+  LRG KV+ + ++ L+KG  VE+ GI+AAQADV
Sbjct: 363 GIQLIPRTLWLDPSGKQLMQWPIEELETLRGSKVKFSRKQDLSKGILVEVKGITAAQADV 422

Query: 361 EVTFSFSSLDKAEPFDPRWV--DAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFF 420
           EVTFSF SL K EPFDP+W+  DA+ +C   GST QGGVGPFGLLTLAS+ L+E+TPVFF
Sbjct: 423 EVTFSFKSLAKREPFDPKWLEYDAEKICSLKGSTVQGGVGPFGLLTLASEKLEEYTPVFF 482

Query: 421 RIFKAHHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSS-KKLALRTLIDHSMVES 480
           R+FKA  N H VL+CSDAT SSL+ +GLY+PSFAGFVD+DL++ KK++LR+LID+S+VES
Sbjct: 483 RVFKA-QNTHKVLMCSDATRSSLK-EGLYRPSFAGFVDVDLATDKKISLRSLIDNSVVES 542

Query: 481 FGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMK 520
           FG  GKTCI+SRVYPT AV+ NAHL+VFNNGSE ITV+NL AWSMK
Sbjct: 543 FGAKGKTCISSRVYPTLAVYENAHLYVFNNGSETITVENLDAWSMK 586

BLAST of MS019512 vs. ExPASy Swiss-Prot
Match: Q39693 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 1.4e-215
Identity = 356/525 (67.81%), Postives = 415/525 (79.05%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           Y F P+  + N   GPM++KG YHLFYQYNPKG+VWGNIVWAHS S+DLINW AL  A+ 
Sbjct: 57  YHFQPQKHWINDPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIF 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGI---DPHNRQVQNYAVPANLSDPYLRDWVKP 120
           PSKPFD  GCWSGSATILPG +PVI+YTGI   DP N QVQNYAVPAN SDP+LR+WVKP
Sbjct: 117 PSKPFDQYGCWSGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKP 176

Query: 121 DFNPVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAK 180
           D NP+V   +  N SAFRDPTT W    GHW+ ++GS R  RG+AYLYRS+DF KW R+ 
Sbjct: 177 DNNPLVGVHT-ENPSAFRDPTTAW-FDGGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSP 236

Query: 181 HPLHSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLE 240
           HP+H+ A TGMWECPDFYPV    + GLD S  G G+KHVLKVSL+ TRY+YYT+G+Y  
Sbjct: 237 HPIHTKAETGMWECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNR 296

Query: 241 NKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWA 300
            +D YVPDNTS DGW GLRYDYGN+YASKTFYD  KKRR+LWGWANESD+  DDV KGWA
Sbjct: 297 VRDFYVPDNTSVDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWA 356

Query: 301 GIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVE 360
           GIQ IPR +WLDP+ +QL+QWP+EEV  LRG ++ M N+KL  G HVE+ GI+AAQADV+
Sbjct: 357 GIQLIPRRIWLDPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVD 416

Query: 361 VTFSFSSLDKAEPFDPRWV--DAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFR 420
            TFSF SLDKAE FDP W+  DAQ VC  +GST QGG+GPFGLLTLASK L+E+TPVFFR
Sbjct: 417 ATFSFKSLDKAESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFR 476

Query: 421 IFKAHHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFG 480
           IFKA   K  VL+CSDA  SSL  +GLYKPSF GFVD+DLS KK++LR+LID+S+VESFG
Sbjct: 477 IFKAEDQKLKVLMCSDAKRSSL-AEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFG 536

Query: 481 GGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKS 521
              K  I+SRVYPT A++ NAHL VFNNG+E ITVDNL AWSM S
Sbjct: 537 AQRKNLISSRVYPTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of MS019512 vs. ExPASy Swiss-Prot
Match: Q39692 (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 PE=3 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 9.1e-215
Identity = 349/525 (66.48%), Postives = 418/525 (79.62%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           Y F PK  + N   GPMY+KG YHLFYQYNPKGAVWGNIVWAHS S+DLINW AL  A+ 
Sbjct: 64  YHFQPKKHWINDPNGPMYYKGFYHLFYQYNPKGAVWGNIVWAHSISKDLINWVALEPAIF 123

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGI---DPHNRQVQNYAVPANLSDPYLRDWVKP 120
           PSKPFD  GCWSGSAT+LPGG+PVIMYTGI    P N QVQN+AVPAN SDPYLR+W+KP
Sbjct: 124 PSKPFDKYGCWSGSATVLPGGKPVIMYTGIVTPSPVNTQVQNFAVPANYSDPYLREWIKP 183

Query: 121 DFNPVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAK 180
           D NP+V   S  N+S+FRDPTT W     HW+ ++GSRR  RG+AYLYRSR+F+KWT+AK
Sbjct: 184 DNNPIVRARS-ENSSSFRDPTTAW-FDGVHWKILVGSRRKHRGIAYLYRSRNFLKWTKAK 243

Query: 181 HPLHSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLE 240
           HPLHS   TGMWEC DFYPV   G  GLDTSV+G  +KHVLKVSL  TRY+YYTVG+Y  
Sbjct: 244 HPLHSKDRTGMWECLDFYPVAPKGMNGLDTSVTGQDIKHVLKVSLYSTRYEYYTVGEYDR 303

Query: 241 NKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWA 300
           + D YVPDNTS DGW GLRYDYGN+YASKTF+D  K+RR+LWGWANESD+ +DDV KGWA
Sbjct: 304 DNDIYVPDNTSVDGWAGLRYDYGNFYASKTFFDPDKQRRILWGWANESDSKQDDVQKGWA 363

Query: 301 GIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVE 360
           GIQ IPR +WLDPN KQL+QWP+EE+  LRG  V M ++ L  G+H+E+ G++AAQADV+
Sbjct: 364 GIQLIPRKLWLDPNGKQLIQWPIEEIQLLRGQNVHMGSQVLNTGEHIEVKGVTAAQADVD 423

Query: 361 VTFSFSSLDKAEPFDPRW--VDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFR 420
            TFSF SLD+AE FDP W  +DA  VC  +GST +GG+GPFG LTLAS+ L+E+TPVFFR
Sbjct: 424 ATFSFKSLDRAEWFDPNWAKLDALDVCDWMGSTVRGGLGPFGFLTLASEKLEEYTPVFFR 483

Query: 421 IFKAHHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFG 480
           +FK   +K  VL+CSDA  SS   +GLYKP FAG+VD+DLS KK++LR+LID+S+VESFG
Sbjct: 484 VFKT-KDKLKVLMCSDAKRSSTTAEGLYKPPFAGYVDVDLSDKKISLRSLIDNSVVESFG 543

Query: 481 GGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKS 521
             G+TCITSRVYP  A++ NAH+ VFNNG+E IT+D+L AWSMK+
Sbjct: 544 AHGRTCITSRVYPKIAIYNNAHVFVFNNGTEAITIDSLDAWSMKA 585

BLAST of MS019512 vs. ExPASy Swiss-Prot
Match: Q8W413 (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3702 GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 3.2e-199
Identity = 323/530 (60.94%), Postives = 401/530 (75.66%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P   + N   GP+Y+KG+YHLFYQYN KGAVWGNI+WAHS S+DL+NW+AL  ALS
Sbjct: 53  FHFQPPKHWINDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALS 112

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSK FDI G WSGS TI+PG  P+I+YTG++ +  Q+QNYA+P + SDPYLR W+KPD N
Sbjct: 113 PSKWFDIGGTWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDN 172

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+  PD  +N SAFRDPTT W  K GHWRTV+GS+R  RG+AY+YRSRDF  W +AKHP+
Sbjct: 173 PIAIPDYTMNGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPV 232

Query: 181 HSVANTGMWECPDFYPVPLTG-KRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENK 240
           HS  +TGMWECPDF+PV LT  + GLD    G   KHVLKVSLD+TRY+YYT+GKY   K
Sbjct: 233 HSKQSTGMWECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKK 292

Query: 241 DRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGI 300
           DRY+PD  +PDGW GLR+DYGN+YASKTF+D  K RR+LWGWANESDT  DD+ KGWAG+
Sbjct: 293 DRYIPDGNTPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGL 352

Query: 301 QAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVT 360
           Q IPRTV LD ++KQL+ WPVEE+  LRG  V M N  +  GQ +E+ GI+ AQADVEVT
Sbjct: 353 QVIPRTVLLDSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVT 412

Query: 361 FSFSSLDKAEPFDPRWV-DAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIF- 420
           F   SL+KAE FDP +      +C   GS  +GGVGPFGL+TLA+  L+E+TPVFFR+F 
Sbjct: 413 FYVGSLEKAEIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFN 472

Query: 421 --KAHHNKHVVLLCSDATSSSLR-------TKGLYKPSFAGFVDIDLSSKKLALRTLIDH 480
             K H  K  VL+CSDA  SSL+          +YKPSFAGFVD+D++  +++LR+LIDH
Sbjct: 473 DTKTHKPK--VLMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDH 532

Query: 481 SMVESFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSM 519
           S+VESFG  GKT ITSRVYP KAV  NAHL+VFNNG++ +T+++L+AW+M
Sbjct: 533 SVVESFGALGKTVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of MS019512 vs. ExPASy Swiss-Prot
Match: Q01IS8 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Oryza sativa subsp. indica OX=39946 GN=CIN3 PE=2 SV=2)

HSP 1 Score: 694.5 bits (1791), Expect = 9.2e-199
Identity = 326/536 (60.82%), Postives = 399/536 (74.44%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           Y F P   + N   GPMY+KG+YHLFYQYNPKGAVWGNI WAHS S DLI+W AL   + 
Sbjct: 50  YHFQPPKHWINDPNGPMYYKGLYHLFYQYNPKGAVWGNIEWAHSVSTDLIDWTALEPGIY 109

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSK FD  GCWSGSAT+LP G PVIMYTGIDP  RQVQN A P NLSDPYLR+W KPD+N
Sbjct: 110 PSKTFDEKGCWSGSATVLPSGVPVIMYTGIDPDERQVQNVAYPVNLSDPYLREWYKPDYN 169

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+++PD G+NASAFRDPTT W G  GHWR ++GS+   +G+A LYRSRDF KW +A HPL
Sbjct: 170 PIINPDGGINASAFRDPTTAWYGPDGHWRLLVGSKVNMKGLAVLYRSRDFKKWVKAHHPL 229

Query: 181 HSVANTGMWECPDFYPVPLTG-----KRGLDT-----SVSGGGVKHVLKVSLDLTRYDYY 240
           HS A+TGMWECPDF+PV + G     +RG+DT     +     VK+VLKVSLDLTRY+YY
Sbjct: 230 HS-AHTGMWECPDFFPVAVAGGSRHYRRGVDTAELHDAAVAEEVKYVLKVSLDLTRYEYY 289

Query: 241 TVGKYLENKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARD 300
           TVG Y    DRYVPD   PD   GLRYDYG++YASK+FYD  K+RR++WGWANESDT  D
Sbjct: 290 TVGWYDHATDRYVPDAAFPDNDYGLRYDYGDFYASKSFYDPAKRRRIVWGWANESDTVPD 349

Query: 301 DVAKGWAGIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGIS 360
           D  KGWAGIQAIPR +WL  + KQL+QWPVEE+  LR   V +T++ + KG + E+ G  
Sbjct: 350 DRRKGWAGIQAIPRKLWLSADGKQLVQWPVEELKALRAKHVNVTDKVIKKGNYFEVTGFK 409

Query: 361 AAQADVEVTFSFSSLDKAEPFDPRW-VDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEF 420
           + Q+DV++ F+   L KAE FDP W  DA+++C +LGS   GGVGPFGL  LAS  L E 
Sbjct: 410 SVQSDVDMAFAIKDLSKAEEFDPAWRTDAEALCKKLGSDVDGGVGPFGLWALASGDLKER 469

Query: 421 TPVFFRIFKAHHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLS-SKKLALRTLIDH 480
           T VFFR+FKA+ + HVVL+C+D T SS  +K +Y+P+FAGFVD+D++ +K++ALRTLIDH
Sbjct: 470 TAVFFRVFKANDSSHVVLMCNDPTRSSYESK-IYRPTFAGFVDVDIAKNKQIALRTLIDH 529

Query: 481 SMVESFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           S+VESFG  GKTCI +RVYP KAV  +AHL VFNNG   + V NL AW MK+ ++N
Sbjct: 530 SVVESFGARGKTCILTRVYPRKAVGDDAHLFVFNNGESDVKVTNLDAWEMKTPKMN 583

BLAST of MS019512 vs. ExPASy TrEMBL
Match: A0A6J1CU71 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Momordica charantia OX=3673 GN=LOC111014380 PE=3 SV=1)

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 512/524 (97.71%), Postives = 515/524 (98.28%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS
Sbjct: 53  FHFQPRRHWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 112

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN
Sbjct: 113 PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 172

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL
Sbjct: 173 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 232

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD
Sbjct: 233 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 292

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ
Sbjct: 293 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 352

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF
Sbjct: 353 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 412

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH
Sbjct: 413 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 472

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGK 
Sbjct: 473 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKA 532

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN
Sbjct: 533 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 576

BLAST of MS019512 vs. ExPASy TrEMBL
Match: A0A6J1FK40 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444940 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 6.3e-267
Identity = 425/524 (81.11%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTG+DP NRQVQNYA+PANLSDPYL DW+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRS+DFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+GK+GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DD++KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+N LRG +V+M+++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDATSSSL+  GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGKT
Sbjct: 477 HN-HVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKT 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFG+AHL+VFNNGSE ITVD LSAWSM+SA +N
Sbjct: 537 CITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 579

BLAST of MS019512 vs. ExPASy TrEMBL
Match: A0A6J1L090 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111498679 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 425/524 (81.11%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ 
Sbjct: 57  FHFQPRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIY 116

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTGIDP NRQVQNYA+PANLSDPYL DW+KPD N
Sbjct: 117 PSKPFDINGCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTDWIKPDDN 176

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDPDSGVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRSRDFVKWT+AKHPL
Sbjct: 177 PIVDPDSGVNASAFRDPTTAWLSDSGHWKTIIGSKRRKRGVAYLYRSRDFVKWTKAKHPL 236

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS  NTGMWECPDF+PVPL+G +GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +D
Sbjct: 237 HSAPNTGMWECPDFFPVPLSGNQGLDVSVTGNLVKHVLKVSLDVTRYEYYTVGKYYHERD 296

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           RYVPDNTS DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DDV+KGWAGIQ
Sbjct: 297 RYVPDNTSVDGWNGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDVSKGWAGIQ 356

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
           AIPRTVWLDPNRKQLLQWPVEE+NRLRG +V+++++KL K QHVE+ GI+AAQADVEVTF
Sbjct: 357 AIPRTVWLDPNRKQLLQWPVEELNRLRGKQVKLSHQKLYKEQHVEVKGITAAQADVEVTF 416

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L+EFTPVFFRIFKAH
Sbjct: 417 SFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLEEFTPVFFRIFKAH 476

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           HN HVVLLCSDA+SSSL+ +GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGK 
Sbjct: 477 HN-HVVLLCSDASSSSLKKEGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKI 536

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTKAVFG+AHL+VFNNG+E ITVD LSAWSM SA VN
Sbjct: 537 CITSRVYPTKAVFGDAHLYVFNNGTEAITVDYLSAWSMTSATVN 579

BLAST of MS019512 vs. ExPASy TrEMBL
Match: A0A1S3B7H3 (beta-fructofuranosidase, insoluble isoenzyme 1 OS=Cucumis melo OX=3656 GN=LOC103486629 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.3e-258
Identity = 414/524 (79.01%), Postives = 464/524 (88.55%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYF GIYHLFYQYNPKGAVWGNIVWAHS SRDLINWK L  A+ 
Sbjct: 53  FHFQPRRNWINDPNGPMYFNGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKAAIY 112

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTGIDP NRQVQNYA+PANLSDPYL +W+KPD N
Sbjct: 113 PSKPFDINGCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDHN 172

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDP  GVNASAFRDPTT W  KSGHW+T+IGS+R  RGMAYLYRSRDFVKWT+AKHPL
Sbjct: 173 PIVDPGPGVNASAFRDPTTAWLSKSGHWKTIIGSKRKRRGMAYLYRSRDFVKWTKAKHPL 232

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS ANTGMWECPDFYPVPL G+ GLD SV+G  VKHVLKVSLDLTRY+YYTVGKY   KD
Sbjct: 233 HSAANTGMWECPDFYPVPLRGRLGLDPSVTGNWVKHVLKVSLDLTRYEYYTVGKYFPKKD 292

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           +YVPDNTS DGW GLRYDYGN+YASK+FYD  +KRRVLWGWANESD+A+DDV+KGWAGIQ
Sbjct: 293 KYVPDNTSVDGWSGLRYDYGNFYASKSFYDPLRKRRVLWGWANESDSAQDDVSKGWAGIQ 352

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
            IPRTVWLD N+KQL+QWPVEE+N LRG KV ++++KL KG  VE+ GI+AAQADVEV F
Sbjct: 353 LIPRTVWLDHNQKQLIQWPVEELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVIF 412

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKAEPFDPRWVDAQ+ C +LG  +QGGVGPFGLLTLAS++L+EFTPVFFRIFKAH
Sbjct: 413 SFSSLDKAEPFDPRWVDAQAACDQLGLKSQGGVGPFGLLTLASENLEEFTPVFFRIFKAH 472

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           H KHVVL+CSDA SSSL+  GLYKP+FAG+VDIDLSSKKL+LR+LIDHS+VESFGGGGKT
Sbjct: 473 H-KHVVLMCSDARSSSLKEDGLYKPAFAGYVDIDLSSKKLSLRSLIDHSVVESFGGGGKT 532

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTK VF +AHLHVFNNG+E ITV+ LSAWSMKSARVN
Sbjct: 533 CITSRVYPTKGVFNDAHLHVFNNGTEAITVEYLSAWSMKSARVN 575

BLAST of MS019512 vs. ExPASy TrEMBL
Match: A0A0A0LAY1 (Cell wall invertase 3 OS=Cucumis sativus OX=3659 GN=Csa_3G777580 PE=2 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.2e-255
Identity = 412/524 (78.63%), Postives = 463/524 (88.36%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P+  + N   GPMYFKGIYHLFYQYNPKGAVWGNIVWAHS SRDLINWK L  A+ 
Sbjct: 55  FHFQPRRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIY 114

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSKPFDINGCWSGSAT+LPG +PVI+YTGIDP NRQVQNYA+PANLSDPYL +W+KPD N
Sbjct: 115 PSKPFDINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNN 174

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+VDP  GVNASAFRDPTT W  KSGHW+TVIGS+R  RGMAYLYRSRDFVKWT+AKHPL
Sbjct: 175 PIVDPGPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPL 234

Query: 181 HSVANTGMWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKD 240
           HS ANTGMWECPDFYPVPL GK GLD SV+G  VKHV KVSLDLTRY+YYTVGKY   KD
Sbjct: 235 HSAANTGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKD 294

Query: 241 RYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQ 300
           +YVPDNTS DGW GLRYDYGN+YASK+FYD  KKRRVLWGWANESD+A+DDV+KGWAGIQ
Sbjct: 295 KYVPDNTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQ 354

Query: 301 AIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTF 360
            IPRTVWLD N++QL QWPV+E+N LRG KV ++++KL KG  VE+ GI+AAQADVEV F
Sbjct: 355 LIPRTVWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMF 414

Query: 361 SFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAH 420
           SFSSLDKA+PFDPRWVDAQ+ C +LG+ +QGGVGPFGLLTLAS++LDEFTPVFFRIFKAH
Sbjct: 415 SFSSLDKADPFDPRWVDAQAACDQLGN-SQGGVGPFGLLTLASENLDEFTPVFFRIFKAH 474

Query: 421 HNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKT 480
           H KHVVL+CSDA SSSL+ +GLYKP+FAG+VDIDLS KKL+LR+LIDHS+VESFGGGGKT
Sbjct: 475 H-KHVVLMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKT 534

Query: 481 CITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           CITSRVYPTK VF +AHLHVFNNG+E ITV+ L AWSMKSAR+N
Sbjct: 535 CITSRVYPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of MS019512 vs. TAIR 10
Match: AT2G36190.1 (cell wall invertase 4 )

HSP 1 Score: 696.0 bits (1795), Expect = 2.2e-200
Identity = 323/530 (60.94%), Postives = 401/530 (75.66%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P   + N   GP+Y+KG+YHLFYQYN KGAVWGNI+WAHS S+DL+NW+AL  ALS
Sbjct: 53  FHFQPPKHWINDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALS 112

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSK FDI G WSGS TI+PG  P+I+YTG++ +  Q+QNYA+P + SDPYLR W+KPD N
Sbjct: 113 PSKWFDIGGTWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDN 172

Query: 121 PVVDPDSGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPL 180
           P+  PD  +N SAFRDPTT W  K GHWRTV+GS+R  RG+AY+YRSRDF  W +AKHP+
Sbjct: 173 PIAIPDYTMNGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPV 232

Query: 181 HSVANTGMWECPDFYPVPLTG-KRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENK 240
           HS  +TGMWECPDF+PV LT  + GLD    G   KHVLKVSLD+TRY+YYT+GKY   K
Sbjct: 233 HSKQSTGMWECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKK 292

Query: 241 DRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGI 300
           DRY+PD  +PDGW GLR+DYGN+YASKTF+D  K RR+LWGWANESDT  DD+ KGWAG+
Sbjct: 293 DRYIPDGNTPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGL 352

Query: 301 QAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVT 360
           Q IPRTV LD ++KQL+ WPVEE+  LRG  V M N  +  GQ +E+ GI+ AQADVEVT
Sbjct: 353 QVIPRTVLLDSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVT 412

Query: 361 FSFSSLDKAEPFDPRWV-DAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIF- 420
           F   SL+KAE FDP +      +C   GS  +GGVGPFGL+TLA+  L+E+TPVFFR+F 
Sbjct: 413 FYVGSLEKAEIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFN 472

Query: 421 --KAHHNKHVVLLCSDATSSSLR-------TKGLYKPSFAGFVDIDLSSKKLALRTLIDH 480
             K H  K  VL+CSDA  SSL+          +YKPSFAGFVD+D++  +++LR+LIDH
Sbjct: 473 DTKTHKPK--VLMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDH 532

Query: 481 SMVESFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSM 519
           S+VESFG  GKT ITSRVYP KAV  NAHL+VFNNG++ +T+++L+AW+M
Sbjct: 533 SVVESFGALGKTVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of MS019512 vs. TAIR 10
Match: AT3G52600.1 (cell wall invertase 2 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.2e-199
Identity = 326/530 (61.51%), Postives = 400/530 (75.47%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           Y F P   + N    PM +KG+YHLFYQYNPKGAVWGNIVWAHS S+DLINW+AL  A+ 
Sbjct: 51  YHFQPPRHWINDPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIY 110

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PSK FDING WSGSAT +PG  PVI+YTGI  +  Q+QNYA+P +LSDPYL+ W+KPD N
Sbjct: 111 PSKWFDINGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDN 170

Query: 121 PVVDPDSGVNASAFRDPTTGW-RGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHP 180
           P+V PD+G N SAFRDPTT W   K G+WR ++GS+R +RG+AY+Y+SRDF KW ++K P
Sbjct: 171 PIVKPDNGENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRP 230

Query: 181 LHSVANTGMWECPDFYPVPLTGKR-GLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLEN 240
           +HS   TGMWECPDF+PV +T K+ GLD S  G   KHVLKVSLDLTRY+YYT+G Y   
Sbjct: 231 IHSRKKTGMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTK 290

Query: 241 KDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAG 300
           KDRY PD  +PDGW GLR+DYGNYYASKTF+D    RR+LWGWANESDT +DD  KGWAG
Sbjct: 291 KDRYRPDGYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAG 350

Query: 301 IQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEV 360
           IQ IPRT+ LD + KQL+ WP+EE+  LRG  V+MTN+K+  GQ  E+ GI+ AQ DV+V
Sbjct: 351 IQLIPRTILLDSSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDV 410

Query: 361 TFSFSSLDKAEPFDPRWVDAQ-SVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIF 420
           TF+  +L+KAE FD  +      +C   GS   GGVGPFGL+TLA+  L+E+TPVFFR+F
Sbjct: 411 TFNVGNLEKAEKFDESFATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVF 470

Query: 421 K-AHHNKHVVLLCSDATSSSLR-------TKGLYKPSFAGFVDIDLSSKKLALRTLIDHS 480
           K A  NK  VL+CSDA  SSL+        + +YKPSFAGFVD+ L   K++LR+LIDHS
Sbjct: 471 KDAASNKPKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHS 530

Query: 481 MVESFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMK 520
           +VESFG  GKT ITSRVYPTKAV   AHL VFNNGS+ +TV++L+AW+M+
Sbjct: 531 VVESFGAKGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of MS019512 vs. TAIR 10
Match: AT3G52600.2 (cell wall invertase 2 )

HSP 1 Score: 682.6 bits (1760), Expect = 2.6e-196
Identity = 321/514 (62.45%), Postives = 394/514 (76.65%), Query Frame = 0

Query: 17  MYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALSPSKPFDINGCWSGSAT 76
           M +KG+YHLFYQYNPKGAVWGNIVWAHS S+DLINW+AL  A+ PSK FDING WSGSAT
Sbjct: 1   MLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSAT 60

Query: 77  ILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFNPVVDPDSGVNASAFRD 136
            +PG  PVI+YTGI  +  Q+QNYA+P +LSDPYL+ W+KPD NP+V PD+G N SAFRD
Sbjct: 61  HVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRD 120

Query: 137 PTTGW-RGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPLHSVANTGMWECPDFY 196
           PTT W   K G+WR ++GS+R +RG+AY+Y+SRDF KW ++K P+HS   TGMWECPDF+
Sbjct: 121 PTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFF 180

Query: 197 PVPLTGKR-GLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKDRYVPDNTSPDGWLG 256
           PV +T K+ GLD S  G   KHVLKVSLDLTRY+YYT+G Y   KDRY PD  +PDGW G
Sbjct: 181 PVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDG 240

Query: 257 LRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQAIPRTVWLDPNRKQ 316
           LR+DYGNYYASKTF+D    RR+LWGWANESDT +DD  KGWAGIQ IPRT+ LD + KQ
Sbjct: 241 LRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQ 300

Query: 317 LLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTFSFSSLDKAEPFDPR 376
           L+ WP+EE+  LRG  V+MTN+K+  GQ  E+ GI+ AQ DV+VTF+  +L+KAE FD  
Sbjct: 301 LVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDES 360

Query: 377 WVDAQ-SVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFK-AHHNKHVVLLCSDA 436
           +      +C   GS   GGVGPFGL+TLA+  L+E+TPVFFR+FK A  NK  VL+CSDA
Sbjct: 361 FATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDA 420

Query: 437 TSSSLR-------TKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKTCITSR 496
             SSL+        + +YKPSFAGFVD+ L   K++LR+LIDHS+VESFG  GKT ITSR
Sbjct: 421 KPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSR 480

Query: 497 VYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMK 520
           VYPTKAV   AHL VFNNGS+ +TV++L+AW+M+
Sbjct: 481 VYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 514

BLAST of MS019512 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 633.6 bits (1633), Expect = 1.4e-181
Identity = 299/532 (56.20%), Postives = 384/532 (72.18%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P   + N   GPM +KGIYHLFYQ+NPKGAVWGNIVWAHS S DLINW   P A+ 
Sbjct: 55  FHFQPPKNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIF 114

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PS PFDINGCWSGSATILP G+PVI+YTGIDP N+QVQN A P NLSDPYLR+W K   N
Sbjct: 115 PSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLN 174

Query: 121 PVVDPD--SGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKH 180
           P++ PD  +G+NAS+FRDPTT W G+   WR +IGS+   RG+A  Y S+DF+KW ++  
Sbjct: 175 PLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPE 234

Query: 181 PLHSVANTGMWECPDFYPVPLTGKRGLDTSVSGGG---VKHVLKVSLDLTRYDYYTVGKY 240
           PLH    +GMWECPDF+PV   G  G++TS  G     +KHVLK+SLD T++DYYT+G Y
Sbjct: 235 PLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTY 294

Query: 241 LENKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKG 300
              KD++VPDN         RYDYG YYASKTF+D+ K RR+LWGW NES +  DDV KG
Sbjct: 295 DRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKG 354

Query: 301 WAGIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVE-MTNRKLAKGQHVEIHGISAAQA 360
           W+GIQ IPR +WLD + KQL+QWPV EV RLR  +V+ + N+ L  G  +E++G++AAQA
Sbjct: 355 WSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQA 414

Query: 361 DVEVTFSFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFF 420
           DVEV F    L+KA+  +P W D Q +C ++  + + G+GPFGL+ LASK+L+E+T V+F
Sbjct: 415 DVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYF 474

Query: 421 RIFKA--HHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVE 480
           RIFKA  + NK+VVL+CSD + SSL+     K ++  FVDI+   + L+LR LIDHS+VE
Sbjct: 475 RIFKARQNSNKYVVLMCSDQSRSSLKEDN-DKTTYGAFVDIN-PHQPLSLRALIDHSVVE 534

Query: 481 SFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           SFGG G+ CITSRVYP  A+  ++HL  FN G + + V NL+AWSM SA+++
Sbjct: 535 SFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of MS019512 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 631.3 bits (1627), Expect = 6.8e-181
Identity = 299/532 (56.20%), Postives = 384/532 (72.18%), Query Frame = 0

Query: 1   YRFDPKGIFENCKTGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALS 60
           + F P    +N   GPM +KGIYHLFYQ+NPKGAVWGNIVWAHS S DLINW   P A+ 
Sbjct: 55  FHFQPP---KNWMNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIF 114

Query: 61  PSKPFDINGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFN 120
           PS PFDINGCWSGSATILP G+PVI+YTGIDP N+QVQN A P NLSDPYLR+W K   N
Sbjct: 115 PSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLN 174

Query: 121 PVVDPD--SGVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKH 180
           P++ PD  +G+NAS+FRDPTT W G+   WR +IGS+   RG+A  Y S+DF+KW ++  
Sbjct: 175 PLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPE 234

Query: 181 PLHSVANTGMWECPDFYPVPLTGKRGLDTSVSGGG---VKHVLKVSLDLTRYDYYTVGKY 240
           PLH    +GMWECPDF+PV   G  G++TS  G     +KHVLK+SLD T++DYYT+G Y
Sbjct: 235 PLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTY 294

Query: 241 LENKDRYVPDNTSPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKG 300
              KD++VPDN         RYDYG YYASKTF+D+ K RR+LWGW NES +  DDV KG
Sbjct: 295 DRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKG 354

Query: 301 WAGIQAIPRTVWLDPNRKQLLQWPVEEVNRLRGMKVE-MTNRKLAKGQHVEIHGISAAQA 360
           W+GIQ IPR +WLD + KQL+QWPV EV RLR  +V+ + N+ L  G  +E++G++AAQA
Sbjct: 355 WSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQA 414

Query: 361 DVEVTFSFSSLDKAEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFF 420
           DVEV F    L+KA+  +P W D Q +C ++  + + G+GPFGL+ LASK+L+E+T V+F
Sbjct: 415 DVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYF 474

Query: 421 RIFKA--HHNKHVVLLCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVE 480
           RIFKA  + NK+VVL+CSD + SSL+     K ++  FVDI+   + L+LR LIDHS+VE
Sbjct: 475 RIFKARQNSNKYVVLMCSDQSRSSLKEDN-DKTTYGAFVDIN-PHQPLSLRALIDHSVVE 534

Query: 481 SFGGGGKTCITSRVYPTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 525
           SFGG G+ CITSRVYP  A+  ++HL  FN G + + V NL+AWSM SA+++
Sbjct: 535 SFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581