MS018954 (gene) Bitter gourd (TR) v1

Overview


Name	MS018954
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	GDSL esterase/lipase
Location	scaffold20: 200107 .. 204959 (-)
RNA-Seq Expression	MS018954
Synteny	MS018954

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGATCCGAAGAAGCTGAATCCCTTCTTGGTTTGTGTCATTTCTCTTGCCATTTTCATTCCATCAAGCTGCAGTGATCTCTCACCTCCAACCTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTCGCTGGCACGGGACTCCCGATCGGCCTCCCCCACGGCATTACGTTCTTCCACCGTGGCACCGGGCGGCTCGGCGACGGCCGCCTCATAATCGACTTCTTTTGTGAGTTCCATATATGAGTAGGGTCTTTGTTTGGGTTTGTTTTGAGATTAGGGAGTGAAAAAAGTTTGATAAATGTTTTGGGATTCAGGTGAAGAGCTGAAATTGAGCTATTTGAGCCCATATTTGGAGTCCTTAGCTCCAAATTTCACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCTACTACACTTCCACAATTTGTCCCTTTTGCTCTTGATATTCAAGTTCGCCAATTCATTCACTTCAAAAATCGTTCTCTTGAGCTTCAATCATTGGGTATGTATGTTGTTCTTGTTTCCGGTTTCGGGTGTTCGTGGATTAGGTCGAATAAAAAGATAGGTTTGAACTCGTTTGAATGAGTTTGAGAGACAAATTTATCAAATTTGAACGATCTTAGATGGGTGATTAATTGGACTTAACCTAATCTAATTATATATTAGTATATTTAGGTTAGTTCAATTTGAGATATTTGCATCGTTGAGTTAGTTCAATGTTGAACTAAATAGTTTTGCGATATCGTAATATATAAAAAAAGTATTTATGAATTATGGTTCATCTCTCGATCTCAGCAATAAAAATGAGAGGAAAATTAGACATTATATCTAAATATATGCTTGAGTTTGAGTTTCAAGAGTTTACAATGATAACGCAAAATTAATTTCAAGCGAATATATGAGCTAGTTCTCACCTGGTAATTGAGTCAGATTATTTAAACCAAAATTGACTCACACAAGTCTATAGTTCAACTAGATCTAAATGACGAGTTTGAGTGCTCATCAAGTCGATAAAATCTTTTCGGGTCATCAAAATTGTTTGAAAACTCGAAGCGTAAATTGCGGCAGGTTATAAAAGAGAGAATTACATGATGGATGAAGAAGGTTTTAGGAATGGTGCATACATGATTGACATTGGACAAAATGATCTGTTGGTTGCCCTCTATGCATCAAATTTAACCTACAAACCAGTGGTCCAAAAGATCCCTTCTTTTGTTGCAGAGATCAAGCTGGCCATTCAGGTGAAACTTCTTCCTCTTTTCCTTTCAGAATTCAACTTCTAAAATATTACAAATTGGCTTCTGTTTTTTGAATTTAATATAAAGCTTCTTGACAAGGAATCATGTGAAGTATTTTTTAATGTGCAATTGAGTATTCTTGGTCTTGAAGGAATCATTTTATTTAATTTTTCCTCCCATTCTTTAATCTTTCTTTAGAAAATGAGTTTGTATGGTGTCATTGTTCTTATGGTCATGTTCAATGAAATCCTAGTCAAATTTATCAATTTAAAAACTTAAAAAGAAGAACTTTAATGTTCCAATAAATGGCTCTCACCTTCCTTAGTCAAAATCTGATAGTTTGGGGCTATAGGAATTCTATTCTTAAGCTACTTAAAGAGTCTATTTGGTAATATTTCGTTTTTCTGTTTAAAATTTTTGAGAAATATAAACCTTTTGTAACTGCTCATGTTTCTCGTTTTTTATTTTTAAGAAATGTTTTTAAAATTGTGTAAAAATTTGGAAAACAACCAAATCAAGTTTATTGTTCTAAACCATCATCCGCGGCTCATTAGTCCAAAGAGAATTGAAAGGGGATGACTAGGTTCAAACCTCATGATATAGCCACTTACCTTAAGAATAAAAAAATTCTTATTTGTTTTTCGGCACCAAATATTGTAGGGTCAGACGGTTGCCCCGTTAGATTAGTAAGCGAGCCCAGACACACTCACGGATTTAAATTTTTTTTATTGTTCTAGTTTTTTTCCATAACACAGATAAAAAAAACATTATTTTTATCATTTTTATTTTTTAAAATGCCTAATATATATCAAAATAAATAAATGTAACATAAAAAATTTGAATATTTACTTTGAAAAATTAAATTTCACATATAACTTTTATTTTTTTAATCTAAAATTTCAGAGAACAAAAAACAAGGAAGGGTTACCATAAACATGTTTTTGTTTATTTTTTCAATAAAAAGTAAAGAACAAGAAACAAGTACTAAATTTGGTTACGTTTTTCTTGACTGAAAGAGAGGAAGCGAGAAACAAAAAATATGAAACTATAAAGAACAAACGATAAGGTTACCAAACATAGAAATCTAAGCAAATTTGTTAACCTAAGCATTTCACAACTATTTATGTTTATTATTTCTCGTTTTTTAAAAACATTTCTCAAAATTGTATAGAATTTCTGGAAACAGCAAGAACAAATTTCCTTCCTTCTTGTTCCCATTTCCAATTTCTTCCACATCACAAACAGAAATATGTTTTATCAATATTCTTGAATTCATGGGTGTTGAGGTCATGCAATTCTATTTTTCTCTATAATTAAAAAGAAGGAAACAGAAACAAAAAATGGGAATGTTATTGGGATTATGGAGCCAAAACAGGAAACCATATCCTATTAAGAAAAAGATTTTTCAACACTATTACCAATTCCACCGATTCCTACTGGGAGTAGGATATTGTTGACTTATAAGGATTACAATATTGTTTTCCTATAAGAATTAGGATATTTGGCTTCTTATAAATAGAGCTTTTATGTTAAGGTTTCATTTGTACAGAATCCAAATAGAAGGGACTTGAGTGGTTGTGGGCAGTGTTGAAAAGAATTCTGTATCCAGTGGGTTTCGTGAGTGTTTTTCATTACTGTACCTAAGTATAGTGTGTTATAATACTTAGAAGCTAGAGGGCTTTTGTTGAGACCTCTAGGGGATATTTTTGGTGAGAGTTTTGTATTCTAATCCTTAAACAATTATTAGTGAATTACCCACATCTGAGTGGAAGACTGAACGTAGCCCTTAGTGGTGAACCAGTATAATTTGATGTGTCGTTATTCTTGTGCTTTTACATTTTTCTTCCTATTTTCATATATTTTGGTTGTGTTTGGATATTTCTGTGGGAGGGGAAATTATTCAGGGTCTTCAATTTCCCAACAAATGTTACCAAACGGACCCTCAAAACTGAATAGGAATACAATCTTAACCTAAATCCTAGCTCGTGAGTTCCCAGTAGAAAATTTCTCCATCTCGGGCTTCGTAATTTCTAGATTTTTTTTATCACTTTGTAATTTCTAGATTTGTAGTAATGAGTTAAGTGGTTATCGGATTAATATTGCACACAGAATTTATATGGCAATGGTGGGAGGAAATTTTGGATACACAACACAGGACCAATTGGATGTTCTCCAAAAGAGTTGGCTTTGCATCCACACAACAAGAGGGACGTTGACCAAATTGGCTGCCTTAGGGTTCACAATCAAGTTGCCAAATCCTTCAACAAAGCCCTAAAAAATGTGTGCAAACAGCTCAGATCTCAGTTGAAGGATGCAACTATTGTTTACGTTGACGTATACACTATCAAGTACAATCTCTTCGCTCATCCCAAAACATATGGTATGATTTTTTTTATTATTATTTCACTTTTATATGTTATATATATATATGTTAGTTAATTAATATGCAAAGTTAATGTGATATATATATGAGGATAAAACTTGGGTGAAGATGCTTAGTCCATAAGATGAGGCTTTTAGAAAAAGAGAGAGAAATTAAAAAGCTATGGGATTTGACCCTTTTAAATGTGACTAGATTTCATTAGTCTAAAGAAGGAATGGTCTTTGTCAAGCTAGCTAGCCAACTATTCCATGAGATAAGAGTTGAAAGATAAAAAGATGGGGCAAAAAAAATTAGGGTTGATCCTAATATATATACTTGATTAGAGTTCGAACTTAGAGATAAACACTAAGAAAGAAGAGAAAAATCAGGAAAAAAAAAAAAGGGAAAGAAGAAAAGGAAAAGAAGTTTGATTATATGGTAGAACACATTGACAAATTTTCTTGGCCTTGTTAGGGAAGTCAAATCTACAAATTCACTAGTAGGGTTTGAAAATGGGAAACAAAAGAAACATAGGGTTTGATCTCTTCTGTGACTTTTAATTTCATTAGTCATTTTTGAGATAGGAAAATTTTAAGTTTAAATATTATTTTGGTTCATGTATTTTCAAAATGTCCATTTTGATTCTCGTAGTTTCAACTTTTGTCCATTTAATCCATGTACTTTTGTAAACTTCTTGTTTTGGTACTTGAACTTAAAAATAATGACTATTTAGGGTCCCTTTATTTTATTTTTATTAATTTTTGTAAAGACTAGGCACGATATTACACTCCATAAATTTCTAGAAACAATGTAGTTATAATTTTGTCTCAAACAAGACTGAAACAATCACTTTTTAAAAGTACAGAGATCAAAGCATAAATTTGTAAAAGTATCCAAATGATCAAAATGGACAAAAGTTGAAAATGCATAGACCAAACTAGCATTTAAACCAAAAATTTTGGTCCACTCGTTTTGTTTCGAAGCATATATGGAATAGAACGATGACGAAAGTGACAACGTGTTTGGTTTGTGAATTTACGAAGGGTTCGAGAATCCATTGATGGGATGTTGTGGGTATGGAGGGCCACCAAACAACTACAATGTGAAGGCAACATGTGGGCAACCTGGTTACTCCCTCTGCGCCAACCCTTCAAAATCCATAGTTTGGGATGGAGTTCATTTCACTGATGCTGCCAATCGCATTGTGGCTTCAGCTATCTTATCTTCCAATTTCTCTACTCCT

mRNA sequence

TTTGATCCGAAGAAGCTGAATCCCTTCTTGGTTTGTGTCATTTCTCTTGCCATTTTCATTCCATCAAGCTGCAGTGATCTCTCACCTCCAACCTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTCGCTGGCACGGGACTCCCGATCGGCCTCCCCCACGGCATTACGTTCTTCCACCGTGGCACCGGGCGGCTCGGCGACGGCCGCCTCATAATCGACTTCTTTTGTGAAGAGCTGAAATTGAGCTATTTGAGCCCATATTTGGAGTCCTTAGCTCCAAATTTCACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCTACTACACTTCCACAATTTGTCCCTTTTGCTCTTGATATTCAAGTTCGCCAATTCATTCACTTCAAAAATCGTTCTCTTGAGCTTCAATCATTGGGTTATAAAAGAGAGAATTACATGATGGATGAAGAAGGTTTTAGGAATGGTGCATACATGATTGACATTGGACAAAATGATCTGTTGGTTGCCCTCTATGCATCAAATTTAACCTACAAACCAGTGGTCCAAAAGATCCCTTCTTTTGTTGCAGAGATCAAGCTGGCCATTCAGAATTTATATGGCAATGGTGGGAGGAAATTTTGGATACACAACACAGGACCAATTGGATGTTCTCCAAAAGAGTTGGCTTTGCATCCACACAACAAGAGGGACGTTGACCAAATTGGCTGCCTTAGGGTTCACAATCAAGTTGCCAAATCCTTCAACAAAGCCCTAAAAAATGTGTGCAAACAGCTCAGATCTCAGTTGAAGGATGCAACTATTGTTTACGTTGACGTATACACTATCAAGTACAATCTCTTCGCTCATCCCAAAACATATGGTATGATTTTTTTTATTATTATTTCACTTTTATATGTTATATATATATATGTTAGGTTCGAGAATCCATTGATGGGATGTTGTGGGTATGGAGGGCCACCAAACAACTACAATGTGAAGGCAACATGTGGGCAACCTGGTTACTCCCTCTGCGCCAACCCTTCAAAATCCATAGTTTGGGATGGAGTTCATTTCACTGATGCTGCCAATCGCATTGTGGCTTCAGCTATCTTATCTTCCAATTTCTCTACTCCT

Coding sequence (CDS)

TTTGATCCGAAGAAGCTGAATCCCTTCTTGGTTTGTGTCATTTCTCTTGCCATTTTCATTCCATCAAGCTGCAGTGATCTCTCACCTCCAACCTTGATCAACTTCGGCGACTCGAACTCCGATACCGGCGGTGTTCTCGCTGGCACGGGACTCCCGATCGGCCTCCCCCACGGCATTACGTTCTTCCACCGTGGCACCGGGCGGCTCGGCGACGGCCGCCTCATAATCGACTTCTTTTGTGAAGAGCTGAAATTGAGCTATTTGAGCCCATATTTGGAGTCCTTAGCTCCAAATTTCACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCTACTACACTTCCACAATTTGTCCCTTTTGCTCTTGATATTCAAGTTCGCCAATTCATTCACTTCAAAAATCGTTCTCTTGAGCTTCAATCATTGGGTTATAAAAGAGAGAATTACATGATGGATGAAGAAGGTTTTAGGAATGGTGCATACATGATTGACATTGGACAAAATGATCTGTTGGTTGCCCTCTATGCATCAAATTTAACCTACAAACCAGTGGTCCAAAAGATCCCTTCTTTTGTTGCAGAGATCAAGCTGGCCATTCAGAATTTATATGGCAATGGTGGGAGGAAATTTTGGATACACAACACAGGACCAATTGGATGTTCTCCAAAAGAGTTGGCTTTGCATCCACACAACAAGAGGGACGTTGACCAAATTGGCTGCCTTAGGGTTCACAATCAAGTTGCCAAATCCTTCAACAAAGCCCTAAAAAATGTGTGCAAACAGCTCAGATCTCAGTTGAAGGATGCAACTATTGTTTACGTTGACGTATACACTATCAAGTACAATCTCTTCGCTCATCCCAAAACATATGGTATGATTTTTTTTATTATTATTTCACTTTTATATGTTATATATATATATGTTAGGTTCGAGAATCCATTGATGGGATGTTGTGGGTATGGAGGGCCACCAAACAACTACAATGTGAAGGCAACATGTGGGCAACCTGGTTACTCCCTCTGCGCCAACCCTTCAAAATCCATAGTTTGGGATGGAGTTCATTTCACTGATGCTGCCAATCGCATTGTGGCTTCAGCTATCTTATCTTCCAATTTCTCTACTCCT

Protein sequence

FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAILSSNFSTP

Homology

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BLAST of MS018954 vs. NCBI nr
Match: XP_022157026.1 (GDSL esterase/lipase At3g62280 [Momordica charantia])

HSP 1 Score: 724.5 bits (1869), Expect = 4.5e-205
Identity = 354/374 (94.65%), Postives = 355/374 (94.92%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPI LPHGIT
Sbjct: 17  FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPISLPHGIT 76

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGRLGDGRLIIDFFCEELKL+YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 77  FFHRGTGRLGDGRLIIDFFCEELKLTYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 136

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY
Sbjct: 137 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 196

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL
Sbjct: 197 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 256

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYG          
Sbjct: 257 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYG---------- 316

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR
Sbjct: 317 --------FENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 372

Query: 361 IVASAILSSNFSTP 375
           IVASAILSSNFSTP
Sbjct: 377 IVASAILSSNFSTP 372

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BLAST of MS018954 vs. NCBI nr
Match: XP_023529129.1 (GDSL esterase/lipase At3g62280-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 631.3 bits (1627), Expect = 5.2e-177
Identity = 304/374 (81.28%), Postives = 327/374 (87.43%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDPK  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 14  FDPKH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 73

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGR GDGRLIIDFFCE+LKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 74  FFHRGTGRFGDGRLIIDFFCEDLKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 133

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG  +   +MDEEGF+ G YMIDIGQNDLLVALYASNLTY
Sbjct: 134 LDVQIRQFIHFKNRSLHLQSLGICKTKNIMDEEGFKKGVYMIDIGQNDLLVALYASNLTY 193

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +D+DQIGCL
Sbjct: 194 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDIDQIGCL 253

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           +VHNQVAKSFNK LKN+CKQLRSQ KDA +VYVDVYTIKYNLFAHP  YG          
Sbjct: 254 KVHNQVAKSFNKDLKNMCKQLRSQFKDAIVVYVDVYTIKYNLFAHPDVYG---------- 313

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANPSK+IVWDGVH+++AANR
Sbjct: 314 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANR 368

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 374 VVASAILSAHFSTP 368

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BLAST of MS018954 vs. NCBI nr
Match: XP_022935258.1 (GDSL esterase/lipase At3g62280-like [Cucurbita moschata])

HSP 1 Score: 628.2 bits (1619), Expect = 4.4e-176
Identity = 307/374 (82.09%), Postives = 328/374 (87.70%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDP+  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 13  FDPRH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 72

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 73  FFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 132

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQNDLLVALYASNLTY
Sbjct: 133 LDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 192

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +DVDQIGCL
Sbjct: 193 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 252

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNK LKNVCK LRSQ KDA +VYVDVYTIKYNLFAHP  YG          
Sbjct: 253 RVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYG---------- 312

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANPSK+IVWDGVH+++AANR
Sbjct: 313 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANR 365

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 373 VVASAILSAHFSTP 365

[Close]

BLAST of MS018954 vs. NCBI nr
Match: XP_022983405.1 (GDSL esterase/lipase At3g62280-like [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.9e-175
Identity = 306/374 (81.82%), Postives = 326/374 (87.17%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDPK  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 24  FDPKH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 83

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFH GTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 84  FFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 143

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQNDLLVALYASNLTY
Sbjct: 144 LDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 203

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +DVDQIGCL
Sbjct: 204 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 263

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNK LKNVCKQLRSQ KDA +VYVDVYTIKYNLF HP  YG          
Sbjct: 264 RVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYG---------- 323

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANP +SIVWDGVH+++AANR
Sbjct: 324 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIVWDGVHYSEAANR 376

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 384 VVASAILSAHFSTP 376

[Close]

BLAST of MS018954 vs. NCBI nr
Match: KAG7018037.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.5 bits (1612), Expect = 2.9e-175
Identity = 305/374 (81.55%), Postives = 328/374 (87.70%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDP+  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 13  FDPRH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 72

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 73  FFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 132

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQNDLLVALYASNL+Y
Sbjct: 133 LDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQNDLLVALYASNLSY 192

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +DVDQIGCL
Sbjct: 193 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 252

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           +VHNQVAKSFNK LKNVCK LRSQ KDA +VYVDVYTIKYNLFAHP  YG          
Sbjct: 253 KVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDAYG---------- 312

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANPSK+IVWDGVH+++AANR
Sbjct: 313 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANR 365

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 373 VVASAILSAHFSTP 365

[Close]

BLAST of MS018954 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 4.3e-134
Identity = 238/368 (64.67%), Postives = 283/368 (76.90%), Query Frame = 0


Query: 9   FLVCVISLAIFIPSSCSDLS--PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGT 68
           FLV  +SL +   S  S  S   P LINFGDSNSDTGGVLAG GLPIGLPHGITFFHRGT
Sbjct: 12  FLVLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGT 71

Query: 69  GRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDIQVR 128
           GRLGDGRLI+DF+CE LK++YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L IQ+R
Sbjct: 72  GRLGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIR 131

Query: 129 QFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVVQK 188
           QF+HFKNRS EL S G +R+  ++D+ GFRN  YMIDIGQNDLL+ALY SNLTY PVV+K
Sbjct: 132 QFVHFKNRSQELISSG-RRD--LIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEK 191

Query: 189 IPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHNQV 248
           IPS + EIK AIQ +Y  GGRKFW+HNTGP+GC+PKELA+H HN  D+D IGC RVHN+V
Sbjct: 192 IPSMLLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEV 251

Query: 249 AKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIYIY 308
           AK+FNK L ++C +LRSQ KDAT+VYVD+Y+IKY L A  K YG                
Sbjct: 252 AKAFNKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYG---------------- 311

Query: 309 VRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAI 368
             F +PLM CCGYGG PNNY+ KATCGQPG ++C + +K+IVWDGVH+T+AANR V  A+
Sbjct: 312 --FVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAIVWDGVHYTEAANRFVVDAV 357

Query: 369 LSSNFSTP 375
           L++ +S P
Sbjct: 372 LTNRYSYP 357

[Close]

BLAST of MS018954 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 5.2e-103
Identity = 187/370 (50.54%), Postives = 250/370 (67.57%), Query Frame = 0


Query: 6   LNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRG 65
           L PF++ ++   + +   C    PP + NFGDSNSDTGG++AG G  IGLP+G +FF R 
Sbjct: 15  LLPFIL-ILRQNLAVAGGCQ--VPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRS 74

Query: 66  TGRLGDGRLIIDFFCEELKLSYLSPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDIQ 125
           TGRL DGRL+IDF C+ L  S L+PYL+SL    F +G NFA+ G++TLP++VPFAL+IQ
Sbjct: 75  TGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQ 134

Query: 126 VRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVV 185
           + QF+HFK+R+LEL S+    +  M+ E GFRN  YMIDIGQND+  + ++  L+Y  VV
Sbjct: 135 LMQFLHFKSRALELASISDPLKEMMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVV 194

Query: 186 QKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHN 245
           + IP+ ++EIK AI+ LY  GGRKFW+HNTGP+GC P++L++  H+K   D+ GCL  +N
Sbjct: 195 KLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKLSM-VHSK-GFDKHGCLATYN 254

Query: 246 QVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIY 305
             AK FN+ L ++C+ LR++LK+A IVYVD+Y IKY+L A+   YG              
Sbjct: 255 AAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYG-------------- 314

Query: 306 IYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVAS 365
               FE PLM CCGYGGPP NYNV  TCG  G   C   S+ I WDG+H+T+ AN IVA 
Sbjct: 315 ----FEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSRFISWDGIHYTETANAIVAM 360

Query: 366 AILSSNFSTP 375
            +LS   STP
Sbjct: 375 KVLSMQHSTP 360

[Close]

BLAST of MS018954 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 6.3e-101
Identity = 182/371 (49.06%), Postives = 247/371 (66.58%), Query Frame = 0


Query: 9   FLVCVISLAIFI---PS-SCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHR 68
           F + ++SLA+ I   PS + S  + P + NFGDSNSDTGG++AG G PIG P+G  FF R
Sbjct: 14  FFITLVSLALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRR 73

Query: 69  GTGRLGDGRLIIDFFCEELKLSYLSPYLESLA-PNFTSGVNFAVSGATTLPQFVPFALDI 128
            TGRL DGRL+IDF C+ L  S L PYL+SL    F +G NFA++G+ TLP+ VPF+L+I
Sbjct: 74  STGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNVPFSLNI 133

Query: 129 QVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPV 188
           QV+QF HFK+RSLEL S     +   +   GF+N  YMIDIGQND +   +A   +Y   
Sbjct: 134 QVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IARSFARGNSYSQT 193

Query: 189 VQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVH 248
           V+ IP  + EIK +I+ LY  GGR+FWIHNTGP+GC P++L++     +D+DQ GCL  +
Sbjct: 194 VKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKSKDLDQHGCLVSY 253

Query: 249 NQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVI 308
           N  A  FN+ L ++C++LR++L+DATI+Y+D+Y IKY+L A+   YG             
Sbjct: 254 NSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYG------------- 313

Query: 309 YIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVA 368
                F++PLM CCGYGG P NYNVK TCG  G ++C   S+ I WDG+H+T+ AN IVA
Sbjct: 314 -----FKSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVA 363

Query: 369 SAILSSNFSTP 375
             +LS ++S P
Sbjct: 374 MKVLSMHYSKP 363

[Close]

BLAST of MS018954 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.3e-77
Identity = 162/392 (41.33%), Postives = 231/392 (58.93%), Query Frame = 0


Query: 5   KLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHR 64
           K+  F V +  ++    S+  D + P+  NFGDSNSDTG ++AG G+ + LP+G   F  
Sbjct: 5   KMKLFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKT 64

Query: 65  GTGRLGDGRLIIDFFCEELKLSYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPF 124
            + R  DGRL+IDF  +E+ L +L+PYL+SL  PNF  G NFA +G+T LP       PF
Sbjct: 65  SSQRFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPF 124

Query: 125 ALDIQVRQFIHFKNRSLELQS-LGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNL 184
           + D+Q+ QFI FK+R++EL S  G K E Y+   + +  G YMIDIGQND+  A Y+   
Sbjct: 125 SFDLQISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK-- 184

Query: 185 TYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIG 244
           T   V+  IPS +   +  ++ LY  GGR  WIHNTGP+GC  + +A    +   +D+ G
Sbjct: 185 TLDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFG 244

Query: 245 CLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFF-III 304
           C+  HNQ AK FN  L  +  + ++Q  DA + YVD+++IK NL A+   +G  F   +I
Sbjct: 245 CVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLI 304

Query: 305 SLLYV-------IYIYVRFENPLMGCCGYGGPPNNYNVKATCGQ----PGYSL----CAN 364
            L ++       I   + FE PLM CCG GG P NY+ + TCGQ     G S+    C +
Sbjct: 305 DLNHLENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACND 364

Query: 365 PSKSIVWDGVHFTDAANRIVASAILSSNFSTP 375
            S+ I WDG+H+T+AAN  V+S IL+  +S P
Sbjct: 365 SSEYINWDGIHYTEAANEFVSSQILTGKYSDP 394

[Close]

BLAST of MS018954 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.1e-72
Identity = 158/387 (40.83%), Postives = 211/387 (54.52%), Query Frame = 0


Query: 6   LNPFLVCVISLAIFIPSSCSDLSP---------PTLINFGDSNSDTGGVLAGTGLPIGLP 65
           +NP L  + +L++      S LSP         P + NFGDSNSDTGG+ A  G   G P
Sbjct: 1   MNPILSSLFALSLL-----SSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFG-QAGPP 60

Query: 66  HGITFFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGA------ 125
           HG +FF    GR  DGRL+IDF  E L L YLS +L+S+  NF+ G NFA +G+      
Sbjct: 61  HGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALN 120

Query: 126 TTLPQ--FVPFALDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQND 185
           +TL Q  F PF+LD+Q  QF +F NRS  ++S G   +  + + + F    Y  DIGQND
Sbjct: 121 STLRQSGFSPFSLDVQFVQFYNFHNRSQTVRSRGGVYKTMLPESDSFSKALYTFDIGQND 180

Query: 186 LLVALYASNLTYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHP 245
            L A Y +N T + V  ++P  +++   AI+N+YG GGR FWIHNTGPIGC    +   P
Sbjct: 181 -LTAGYFANKTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFP 240

Query: 246 HNKRDVDQIGCLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKT 305
           +   D D  GC+   N +A+ FN ALK    +LRS L +A I YVDVY++K+ LF H + 
Sbjct: 241 NKASDFDSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQG 300

Query: 306 YGMIFFIIISLLYVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGY---------SL 365
           +G                  F+  L+ CCG+GG   NYN    CG               
Sbjct: 301 HG------------------FKGSLVSCCGHGG-KYNYNKGIGCGMKKIVKGKEVYIGKP 360

Query: 366 CANPSKSIVWDGVHFTDAANRIVASAI 367
           C  P K++VWDGVHFT AAN+ +   I
Sbjct: 361 CDEPDKAVVWDGVHFTQAANKFIFDKI 361

[Close]

BLAST of MS018954 vs. ExPASy TrEMBL
Match: A0A6J1DWR3 (GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE=3 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.2e-205
Identity = 354/374 (94.65%), Postives = 355/374 (94.92%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPI LPHGIT
Sbjct: 17  FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPISLPHGIT 76

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGRLGDGRLIIDFFCEELKL+YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 77  FFHRGTGRLGDGRLIIDFFCEELKLTYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 136

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY
Sbjct: 137 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 196

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL
Sbjct: 197 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 256

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYG          
Sbjct: 257 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYG---------- 316

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR
Sbjct: 317 --------FENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 372

Query: 361 IVASAILSSNFSTP 375
           IVASAILSSNFSTP
Sbjct: 377 IVASAILSSNFSTP 372

[Close]

BLAST of MS018954 vs. ExPASy TrEMBL
Match: A0A6J1FA19 (GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC111442195 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.1e-176
Identity = 307/374 (82.09%), Postives = 328/374 (87.70%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDP+  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 13  FDPRH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 72

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFHRGTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 73  FFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 132

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQNDLLVALYASNLTY
Sbjct: 133 LDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 192

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +DVDQIGCL
Sbjct: 193 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 252

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNK LKNVCK LRSQ KDA +VYVDVYTIKYNLFAHP  YG          
Sbjct: 253 RVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYG---------- 312

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANPSK+IVWDGVH+++AANR
Sbjct: 313 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIVWDGVHYSEAANR 365

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 373 VVASAILSAHFSTP 365

[Close]

BLAST of MS018954 vs. ExPASy TrEMBL
Match: A0A6J1J5S7 (GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.4e-175
Identity = 306/374 (81.82%), Postives = 326/374 (87.17%), Query Frame = 0


Query: 1   FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60
           FDPK  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 24  FDPKH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 83

Query: 61  FFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120
           FFH GTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 84  FFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 143

Query: 121 LDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTY 180
           LD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQNDLLVALYASNLTY
Sbjct: 144 LDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 203

Query: 181 KPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCL 240
           KPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHPH  +DVDQIGCL
Sbjct: 204 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 263

Query: 241 RVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLL 300
           RVHNQVAKSFNK LKNVCKQLRSQ KDA +VYVDVYTIKYNLF HP  YG          
Sbjct: 264 RVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYG---------- 323

Query: 301 YVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANR 360
                   FE  LM CCGYGG PNNYNVKATCGQPGYS+CANP +SIVWDGVH+++AANR
Sbjct: 324 --------FEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIVWDGVHYSEAANR 376

Query: 361 IVASAILSSNFSTP 375
           +VASAILS++FSTP
Sbjct: 384 VVASAILSAHFSTP 376

[Close]

BLAST of MS018954 vs. ExPASy TrEMBL
Match: A0A2I4G2K2 (GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV=2)

HSP 1 Score: 543.9 bits (1400), Expect = 5.3e-151
Identity = 258/364 (70.88%), Postives = 301/364 (82.69%), Query Frame = 0


Query: 11  VCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRLG 70
           VCV  L +F  S CS    P LINFGDSNSDTGGVLAGTGLPIGLPHGITFFH GTGRLG
Sbjct: 16  VCVF-LGMFANSKCSSNIRPILINFGDSNSDTGGVLAGTGLPIGLPHGITFFHSGTGRLG 75

Query: 71  DGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDIQVRQFIH 130
           DGRL+IDF CE L LSYLSPYL+SL+PNFTSGVNFAVSGAT LPQFVPFALD+QVRQFI 
Sbjct: 76  DGRLVIDFVCEHLNLSYLSPYLDSLSPNFTSGVNFAVSGATLLPQFVPFALDVQVRQFIR 135

Query: 131 FKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVVQKIPSF 190
           F+ RSLEL SLG +    ++DE+G RN  YMIDIG+NDLL+ALYA+NLTY PV++K+PSF
Sbjct: 136 FRKRSLELLSLGSRN---LIDEDGIRNALYMIDIGENDLLLALYATNLTYAPVIEKVPSF 195

Query: 191 VAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHNQVAKSF 250
           +AEIKLAIQN+Y  GGR+FWIHNTGP+GC+PKELALHPHN  D+D+IGCLR+HN VAK+F
Sbjct: 196 LAEIKLAIQNIYQYGGRRFWIHNTGPLGCAPKELALHPHNNTDLDRIGCLRIHNDVAKAF 255

Query: 251 NKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIYIYVRFE 310
           N+ + NVCK+LRS  KDATIVYVD+YTIKY LFA  K YG                  FE
Sbjct: 256 NEGVNNVCKELRSVFKDATIVYVDIYTIKYKLFAKYKKYG------------------FE 315

Query: 311 NPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAILSSN 370
           +P + CCGYGGPPNNYNVKATCGQPGYS+C N S+SIVWDGVH+T+AANR++A++ILS++
Sbjct: 316 DPFVACCGYGGPPNNYNVKATCGQPGYSICRNVSRSIVWDGVHYTEAANRVIATSILSTH 357

Query: 371 FSTP 375
           ++TP
Sbjct: 376 YTTP 357

[Close]

BLAST of MS018954 vs. ExPASy TrEMBL
Match: A0A5D2TWH5 (Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_D08G152000v1 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.8e-149
Identity = 258/361 (71.47%), Postives = 297/361 (82.27%), Query Frame = 0


Query: 14  ISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRLGDGR 73
           + LA+   S CS L  P +INFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRLGDGR
Sbjct: 18  VFLAMIDVSKCS-LGRPLIINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGTGRLGDGR 77

Query: 74  LIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDIQVRQFIHFKN 133
           LIIDFFCE L LSYLSPYL+SLAPNFTSGVNFAVSGA TLPQFVPF LD+QVRQFI FKN
Sbjct: 78  LIIDFFCEHLNLSYLSPYLDSLAPNFTSGVNFAVSGAMTLPQFVPFVLDVQVRQFIRFKN 137

Query: 134 RSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVVQKIPSFVAE 193
           RS+ELQ  G       +DE+GFR+  YMIDIGQNDLL+ALYASNLTY+PV ++IPSF+AE
Sbjct: 138 RSIELQKTG---TGSFIDEKGFRSAVYMIDIGQNDLLMALYASNLTYEPVAKQIPSFLAE 197

Query: 194 IKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHNQVAKSFNKA 253
           IKLAIQN+Y  GGRKFWIHNTGP+GC+PKELALHPHNK D+D+IGC RVHN +AK+FN+ 
Sbjct: 198 IKLAIQNIYSYGGRKFWIHNTGPLGCAPKELALHPHNKTDLDRIGCFRVHNDLAKAFNRG 257

Query: 254 LKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIYIYVRFENPL 313
           L+N+CK++R+ LKDATIVYVDVY+IKY LFA  K YG                  FE+P 
Sbjct: 258 LRNICKEMRTILKDATIVYVDVYSIKYKLFAKYKKYG------------------FEDPF 317

Query: 314 MGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAILSSNFST 373
           M CCGYGGPPNNY+ KATCGQPG ++C N S+SIVWDGVH+T+A+NR+VA+ ILS  +ST
Sbjct: 318 MACCGYGGPPNNYSQKATCGQPGSTICNNVSRSIVWDGVHYTEASNRVVATTILSGRYST 356

Query: 374 P 375
           P
Sbjct: 378 P 356

[Close]

BLAST of MS018954 vs. TAIR 10
Match: AT3G62280.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 479.2 bits (1232), Expect = 3.1e-135
Identity = 238/368 (64.67%), Postives = 283/368 (76.90%), Query Frame = 0


Query: 9   FLVCVISLAIFIPSSCSDLS--PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRGT 68
           FLV  +SL +   S  S  S   P LINFGDSNSDTGGVLAG GLPIGLPHGITFFHRGT
Sbjct: 12  FLVLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGT 71

Query: 69  GRLGDGRLIIDFFCEELKLSYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDIQVR 128
           GRLGDGRLI+DF+CE LK++YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L IQ+R
Sbjct: 72  GRLGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIR 131

Query: 129 QFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVVQK 188
           QF+HFKNRS EL S G +R+  ++D+ GFRN  YMIDIGQNDLL+ALY SNLTY PVV+K
Sbjct: 132 QFVHFKNRSQELISSG-RRD--LIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEK 191

Query: 189 IPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHNQV 248
           IPS + EIK AIQ +Y  GGRKFW+HNTGP+GC+PKELA+H HN  D+D IGC RVHN+V
Sbjct: 192 IPSMLLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEV 251

Query: 249 AKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIYIY 308
           AK+FNK L ++C +LRSQ KDAT+VYVD+Y+IKY L A  K YG                
Sbjct: 252 AKAFNKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYG---------------- 311

Query: 309 VRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVASAI 368
             F +PLM CCGYGG PNNY+ KATCGQPG ++C + +K+IVWDGVH+T+AANR V  A+
Sbjct: 312 --FVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAIVWDGVHYTEAANRFVVDAV 357

Query: 369 LSSNFSTP 375
           L++ +S P
Sbjct: 372 LTNRYSYP 357

[Close]

BLAST of MS018954 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.7e-104
Identity = 187/370 (50.54%), Postives = 250/370 (67.57%), Query Frame = 0


Query: 6   LNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRG 65
           L PF++ ++   + +   C    PP + NFGDSNSDTGG++AG G  IGLP+G +FF R 
Sbjct: 15  LLPFIL-ILRQNLAVAGGCQ--VPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRS 74

Query: 66  TGRLGDGRLIIDFFCEELKLSYLSPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDIQ 125
           TGRL DGRL+IDF C+ L  S L+PYL+SL    F +G NFA+ G++TLP++VPFAL+IQ
Sbjct: 75  TGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQ 134

Query: 126 VRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPVV 185
           + QF+HFK+R+LEL S+    +  M+ E GFRN  YMIDIGQND+  + ++  L+Y  VV
Sbjct: 135 LMQFLHFKSRALELASISDPLKEMMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVV 194

Query: 186 QKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVHN 245
           + IP+ ++EIK AI+ LY  GGRKFW+HNTGP+GC P++L++  H+K   D+ GCL  +N
Sbjct: 195 KLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKLSM-VHSK-GFDKHGCLATYN 254

Query: 246 QVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVIY 305
             AK FN+ L ++C+ LR++LK+A IVYVD+Y IKY+L A+   YG              
Sbjct: 255 AAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYG-------------- 314

Query: 306 IYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVAS 365
               FE PLM CCGYGGPP NYNV  TCG  G   C   S+ I WDG+H+T+ AN IVA 
Sbjct: 315 ----FEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSRFISWDGIHYTETANAIVAM 360

Query: 366 AILSSNFSTP 375
            +LS   STP
Sbjct: 375 KVLSMQHSTP 360

[Close]

BLAST of MS018954 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 4.5e-102
Identity = 182/371 (49.06%), Postives = 247/371 (66.58%), Query Frame = 0


Query: 9   FLVCVISLAIFI---PS-SCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHR 68
           F + ++SLA+ I   PS + S  + P + NFGDSNSDTGG++AG G PIG P+G  FF R
Sbjct: 14  FFITLVSLALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRR 73

Query: 69  GTGRLGDGRLIIDFFCEELKLSYLSPYLESLA-PNFTSGVNFAVSGATTLPQFVPFALDI 128
            TGRL DGRL+IDF C+ L  S L PYL+SL    F +G NFA++G+ TLP+ VPF+L+I
Sbjct: 74  STGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNVPFSLNI 133

Query: 129 QVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLTYKPV 188
           QV+QF HFK+RSLEL S     +   +   GF+N  YMIDIGQND +   +A   +Y   
Sbjct: 134 QVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IARSFARGNSYSQT 193

Query: 189 VQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGCLRVH 248
           V+ IP  + EIK +I+ LY  GGR+FWIHNTGP+GC P++L++     +D+DQ GCL  +
Sbjct: 194 VKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKSKDLDQHGCLVSY 253

Query: 249 NQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISLLYVI 308
           N  A  FN+ L ++C++LR++L+DATI+Y+D+Y IKY+L A+   YG             
Sbjct: 254 NSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYG------------- 313

Query: 309 YIYVRFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIVWDGVHFTDAANRIVA 368
                F++PLM CCGYGG P NYNVK TCG  G ++C   S+ I WDG+H+T+ AN IVA
Sbjct: 314 -----FKSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVA 363

Query: 369 SAILSSNFSTP 375
             +LS ++S P
Sbjct: 374 MKVLSMHYSKP 363

[Close]

BLAST of MS018954 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.7e-80
Identity = 161/383 (42.04%), Postives = 227/383 (59.27%), Query Frame = 0


Query: 7   NPFLVCVISLAIFIPSSCS-DLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHRG 66
           N  +V  I +++F+PSS S  L  P +INFGDSNSDTG +++     +  P+G T+F+  
Sbjct: 7   NLSIVLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLP 66

Query: 67  TGRLGDGRLIIDFFCEELKLSYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFA 126
           +GR  DGRLI+DF  +E+ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+
Sbjct: 67  SGRYCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFS 126

Query: 127 LDIQVRQFIHFKNRSLELQS-LGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNLT 186
            D+Q+ QFI FK+R++EL S  G K E Y+   + +  G YMIDIGQND+  A Y+   T
Sbjct: 127 FDLQISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--T 186

Query: 187 YKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIGC 246
              V+  IPS +   +  ++ LY  GGR  WIHNTGP+GC  + +A    +   +D+ GC
Sbjct: 187 LDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGC 246

Query: 247 LRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIISL 306
           +  HNQ AK FN  L  +  + ++Q  DA + YVD+++IK NL A+   +G         
Sbjct: 247 VSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFG--------- 306

Query: 307 LYVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQ----PGYSL----CANPSKSIVWDG 366
                    FE PLM CCG GG P NY+ + TCGQ     G S+    C + S+ I WDG
Sbjct: 307 ---------FEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDG 366

Query: 367 VHFTDAANRIVASAILSSNFSTP 375
           +H+T+AAN  V+S IL+  +S P
Sbjct: 367 IHYTEAANEFVSSQILTGKYSDP 369

[Close]

BLAST of MS018954 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 1.8e-79
Identity = 158/384 (41.15%), Postives = 223/384 (58.07%), Query Frame = 0


Query: 5   KLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHR 64
           K+  F V +  ++    S+  D + P+  NFGDSNSDTG ++AG G+ + LP+G   F  
Sbjct: 5   KMKLFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKT 64

Query: 65  GTGRLGDGRLIIDFFCEELKLSYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPF 124
            + R  DGRL+IDF  +E+ L +L+PYL+SL  PNF  G NFA +G+T LP       PF
Sbjct: 65  SSQRFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPF 124

Query: 125 ALDIQVRQFIHFKNRSLELQS-LGYKRENYMMDEEGFRNGAYMIDIGQNDLLVALYASNL 184
           + D+Q+ QFI FK+R++EL S  G K E Y+   + +  G YMIDIGQND+  A Y+   
Sbjct: 125 SFDLQISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK-- 184

Query: 185 TYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHPHNKRDVDQIG 244
           T   V+  IPS +   +  ++ LY  GGR  WIHNTGP+GC  + +A    +   +D+ G
Sbjct: 185 TLDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFG 244

Query: 245 CLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKTYGMIFFIIIS 304
           C+  HNQ AK FN  L  +  + ++Q  DA + YVD+++IK NL A+   +G        
Sbjct: 245 CVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFG-------- 304

Query: 305 LLYVIYIYVRFENPLMGCCGYGGPPNNYNVKATCGQ----PGYSL----CANPSKSIVWD 364
                     FE PLM CCG GG P NY+ + TCGQ     G S+    C + S+ I WD
Sbjct: 305 ----------FEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWD 364

Query: 365 GVHFTDAANRIVASAILSSNFSTP 375
           G+H+T+AAN  V+S IL+  +S P
Sbjct: 365 GIHYTEAANEFVSSQILTGKYSDP 368

The following BLAST results are available for this feature:

BLAST of MS018954 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157026.1	4.5e-205	94.65	GDSL esterase/lipase At3g62280 [Momordica charantia]	[more]
XP_023529129.1	5.2e-177	81.28	GDSL esterase/lipase At3g62280-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935258.1	4.4e-176	82.09	GDSL esterase/lipase At3g62280-like [Cucurbita moschata]	[more]
XP_022983405.1	2.9e-175	81.82	GDSL esterase/lipase At3g62280-like [Cucurbita maxima]	[more]
KAG7018037.1	2.9e-175	81.55	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS018954 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NLP7	4.3e-134	64.67	GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2...	[more]
O80522	5.2e-103	50.54	GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2...	[more]
Q9FXB6	6.3e-101	49.06	GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1	[more]
Q3ECP6	1.3e-77	41.33	GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2...	[more]
Q9FXE5	2.1e-72	40.83	Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1	[more]

BLAST of MS018954 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWR3	2.2e-205	94.65	GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE...	[more]
A0A6J1FA19	2.1e-176	82.09	GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC11144219...	[more]
A0A6J1J5S7	1.4e-175	81.82	GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 ...	[more]
A0A2I4G2K2	5.3e-151	70.88	GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV...	[more]
A0A5D2TWH5	3.8e-149	71.47	Uncharacterized protein OS=Gossypium mustelinum OX=34275 GN=E1A91_D08G152000v1 P...	[more]

BLAST of MS018954 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62280.1	3.1e-135	64.67	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G09390.1	3.7e-104	50.54	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G56670.1	4.5e-102	49.06	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G54790.3	3.7e-80	42.04	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G54790.1	1.8e-79	41.15	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 251..271
None	No IPR available	PANTHER	PTHR22835:SF507	BNAC06G18430D PROTEIN	coord: 10..374
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 10..374
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 28..367
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 27..370 e-value: 3.2E-67 score: 229.2
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 33..366 e-value: 7.0E-40 score: 137.8
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 30..370 e-value: 5.20453E-110 score: 322.643

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018954.1	MS018954.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds