MS018950 (gene) Bitter gourd (TR) v1

Overview
NameMS018950
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase superfamily protein
Locationscaffold20: 135501 .. 137891 (+)
RNA-Seq ExpressionMS018950
SyntenyMS018950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCTCGGTGAGCGTCGTTCCAGCTTCTGCAATACCGGACCATGGTGGGAGCACAATTGGTGTTGAAAGGTTGCCTGATGAGATGAATGATATGAAACTTAGGGATGACAAGGTACCTGCATTTTGTTTACTTTCTGTGCCACTAGCTACTAAACTTACTGTGATCTTGTTCATACAAGTTCTGTTACAGTTTTCTTAAATATGTATTTGGATTCACAGGAAATAGAAGCCACAGTTGTTGACGGGAACGGTACTGAAACAGGCCATATAATTGTAACAACCATTGGTGGAAAGAATGGCCAACCAAAACAGGTGATTATCAAAAAACTTATACCCGGGGCTACTGTAATTTGACTTCCTTCCTGACACTTTTTCTTTTGTAACTTGTTTTAGACCATCAGTTATATGGCCGAGCGCGCTGTAGGACATGGCTCTTTTGGAGTTGTATTTCAGGTAAGTTCTTCACTTCTTTTACAAGCTTTCTAATTGCTTGTTTTCTATCAGTGGATGACTTTTTTCATTTTTATTGTTAAGAAACAAGATACATTATCGATGTTGTTTTGCAGGCAAAATGCTTGGAAACTGGTGAAACAGTTGCCATAAAGAAGGTTCTTCAGGACAAGAGGTACAAGAACAGGGAGCTGCAGACCATGCGCCTTCTTGACCACCCAAATGTTGTGTCCCTAAAGCACTGTTTCTTCTCAACAACTGAGAAGGATGAGCTTTACCTTAATCTTGTGCTTGAGTATGTCCCGGAGACTGTTCATAGAGTGATCAAACATTATAACAAGATGAACCAAAGGATGCCGCTGATATACGTGAAACTCTATTTCTACCAGGTTTTTGCCAAACAAATTTCTCCATTTTTGTGCATATAGGATAGTTGAGATTCGTATTGGACTCCACACATACAACCTACTCTGAATAAGATGAACTCAGCTAATAGTTGTTTTGGGAATATCGTTCCGTTCTGCTTTAACATTCCCTTTTTCTTTCTTTCGTTTCTATGCAGATTTGCAGATCTCTTGCATACATCCATAATAGTATTGGCGTATGCCATAGAGACATAAAGCCTCAAAACTTACTGGTTTGTGTTTATTCCGTTGTAACTGTTGGTTATTTTTATATTTTGATAGACAATTTGTTTATTCTATTTCAAGACCATAAACTTCTACTTGGTTATTGATATTTCCTTCTGCTGATCGTTTTCACTTTGTCCCTTTGACAGGTTAATCCCCATACCCATCAACTCAAACTTTGTGACTTTGGCAGTGCAAAAGTTTTGGTTCGTTTCCCTTGCATTCCGGAATATACTTGATGCGTTCTGCTCTCTCCTCTCGTTGTTGAGACTAATTTAATTTTGCATCTTGCAGGTTAGAGGTGAACCAAATATAGCATACATTTGTTCTAGGTACTATAGAGCCCCCGAACTTATATTTGGTGCAACTGAATACACCTCAGCCATTGACATCTGGTCAGCAGGTTGCGTTCTAGCTGAATTACTTCTTGGACAGGTTTGTAGATTTTCTCCACGCAACTGTCTAATAACTATTGTTCTCAGGTATGAGTCTAAGACATGTTCTCTCTTTTTTCAGCCGCTATTTCCTGGTGAAAGCGGAGTTGATCAGCTTGTCGAAATAATTAAGGTATTACATGCTTTAAGTTCCCTTCCAGAGAACTGTCGAGGTTTTTTCTTTTGCAATCTTTTCTGTTGTTTTGTGTCTCATTTCAATTGTTAATTTGCCAACTCTTGCAGGTTCTAGGAACTCCAACCAGGGAGGAAATTAAGTGTATGAACCCTAATTATACAGAATTCAAGTTTCCCCAAATTAAAGCTCATCCATGGCACAAGGTACTTAAAGTTGAAACCATGTCTGTATTTTTACTTACCCTTCTAAAAAATTTACTGATCTAACATTGTATACATCCACGATATCAGATATTCCACAAGAGAATGCCTCCCGAAGCCGTGGATCTCGTTTCAAGACTACTCCAGTACTCTCCAAACCTCCGAAGCACAGCGGTTAGTAGAAATACTCTAAAATGTTAATATATGAACCGAAATGGCCATTTGGGTATCTGACTAACGTTCCTCATCAATACAGTTGGAGGCTCTGATCCATCCATTCTTCGACGAGCTTCGTGACCCCAATACCCGTTTACCAAATGGACGCTTCCTTCCTCCATTGTTCAACTTTAAGGCTCAGGGTAACGGAAAATGAACTATTAATGAATTACTTGATACCGACTGTCGATCCATCTGTGATGATGCCTAACTTCGATTTTGTTTTGTTTGTGTTGATGATCAGAACTGAAGGGAGTACCCCCTGAGATGCTCGTGAGGCTTATTCCGGAACACGCAAGGAAGCACTGCAATTTTCTCGGATCT

mRNA sequence

ATGACCTCGGTGAGCGTCGTTCCAGCTTCTGCAATACCGGACCATGGTGGGAGCACAATTGGTGTTGAAAGGTTGCCTGATGAGATGAATGATATGAAACTTAGGGATGACAAGGAAATAGAAGCCACAGTTGTTGACGGGAACGGTACTGAAACAGGCCATATAATTGTAACAACCATTGGTGGAAAGAATGGCCAACCAAAACAGACCATCAGTTATATGGCCGAGCGCGCTGTAGGACATGGCTCTTTTGGAGTTGTATTTCAGGCAAAATGCTTGGAAACTGGTGAAACAGTTGCCATAAAGAAGGTTCTTCAGGACAAGAGGTACAAGAACAGGGAGCTGCAGACCATGCGCCTTCTTGACCACCCAAATGTTGTGTCCCTAAAGCACTGTTTCTTCTCAACAACTGAGAAGGATGAGCTTTACCTTAATCTTGTGCTTGAGTATGTCCCGGAGACTGTTCATAGAGTGATCAAACATTATAACAAGATGAACCAAAGGATGCCGCTGATATACGTGAAACTCTATTTCTACCAGATTTGCAGATCTCTTGCATACATCCATAATAGTATTGGCGTATGCCATAGAGACATAAAGCCTCAAAACTTACTGGTTAATCCCCATACCCATCAACTCAAACTTTGTGACTTTGGCAGTGCAAAAGTTTTGGTTAGAGGTGAACCAAATATAGCATACATTTGTTCTAGGTACTATAGAGCCCCCGAACTTATATTTGGTGCAACTGAATACACCTCAGCCATTGACATCTGGTCAGCAGGTTGCGTTCTAGCTGAATTACTTCTTGGACAGCCGCTATTTCCTGGTGAAAGCGGAGTTGATCAGCTTGTCGAAATAATTAAGGTTCTAGGAACTCCAACCAGGGAGGAAATTAAGTGTATGAACCCTAATTATACAGAATTCAAGTTTCCCCAAATTAAAGCTCATCCATGGCACAAGATATTCCACAAGAGAATGCCTCCCGAAGCCGTGGATCTCGTTTCAAGACTACTCCAGTACTCTCCAAACCTCCGAAGCACAGCGTTGGAGGCTCTGATCCATCCATTCTTCGACGAGCTTCGTGACCCCAATACCCGTTTACCAAATGGACGCTTCCTTCCTCCATTGTTCAACTTTAAGGCTCAGGAACTGAAGGGAGTACCCCCTGAGATGCTCGTGAGGCTTATTCCGGAACACGCAAGGAAGCACTGCAATTTTCTCGGATCT

Coding sequence (CDS)

ATGACCTCGGTGAGCGTCGTTCCAGCTTCTGCAATACCGGACCATGGTGGGAGCACAATTGGTGTTGAAAGGTTGCCTGATGAGATGAATGATATGAAACTTAGGGATGACAAGGAAATAGAAGCCACAGTTGTTGACGGGAACGGTACTGAAACAGGCCATATAATTGTAACAACCATTGGTGGAAAGAATGGCCAACCAAAACAGACCATCAGTTATATGGCCGAGCGCGCTGTAGGACATGGCTCTTTTGGAGTTGTATTTCAGGCAAAATGCTTGGAAACTGGTGAAACAGTTGCCATAAAGAAGGTTCTTCAGGACAAGAGGTACAAGAACAGGGAGCTGCAGACCATGCGCCTTCTTGACCACCCAAATGTTGTGTCCCTAAAGCACTGTTTCTTCTCAACAACTGAGAAGGATGAGCTTTACCTTAATCTTGTGCTTGAGTATGTCCCGGAGACTGTTCATAGAGTGATCAAACATTATAACAAGATGAACCAAAGGATGCCGCTGATATACGTGAAACTCTATTTCTACCAGATTTGCAGATCTCTTGCATACATCCATAATAGTATTGGCGTATGCCATAGAGACATAAAGCCTCAAAACTTACTGGTTAATCCCCATACCCATCAACTCAAACTTTGTGACTTTGGCAGTGCAAAAGTTTTGGTTAGAGGTGAACCAAATATAGCATACATTTGTTCTAGGTACTATAGAGCCCCCGAACTTATATTTGGTGCAACTGAATACACCTCAGCCATTGACATCTGGTCAGCAGGTTGCGTTCTAGCTGAATTACTTCTTGGACAGCCGCTATTTCCTGGTGAAAGCGGAGTTGATCAGCTTGTCGAAATAATTAAGGTTCTAGGAACTCCAACCAGGGAGGAAATTAAGTGTATGAACCCTAATTATACAGAATTCAAGTTTCCCCAAATTAAAGCTCATCCATGGCACAAGATATTCCACAAGAGAATGCCTCCCGAAGCCGTGGATCTCGTTTCAAGACTACTCCAGTACTCTCCAAACCTCCGAAGCACAGCGTTGGAGGCTCTGATCCATCCATTCTTCGACGAGCTTCGTGACCCCAATACCCGTTTACCAAATGGACGCTTCCTTCCTCCATTGTTCAACTTTAAGGCTCAGGAACTGAAGGGAGTACCCCCTGAGATGCTCGTGAGGCTTATTCCGGAACACGCAAGGAAGCACTGCAATTTTCTCGGATCT

Protein sequence

MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTIGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYRAPELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDELRDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS
Homology
BLAST of MS018950 vs. NCBI nr
Match: XP_022155240.1 (glycogen synthase kinase-3 homolog MsK-3 [Momordica charantia])

HSP 1 Score: 842.0 bits (2174), Expect = 2.1e-240
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. NCBI nr
Match: XP_022935403.1 (glycogen synthase kinase-3 homolog MsK-3-like [Cucurbita moschata] >XP_023528441.1 glycogen synthase kinase-3 homolog MsK-3-like [Cucurbita pepo subsp. pepo] >KAG7018040.1 Glycogen synthase kinase-3-like MsK-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.6 bits (2139), Expect = 2.4e-236
Identity = 401/409 (98.04%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVP S I DHGGSTIGV+RLPDEMNDM++RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. NCBI nr
Match: XP_023523975.1 (glycogen synthase kinase-3 homolog MsK-3 [Cucurbita pepo subsp. pepo] >KAG7036621.1 Glycogen synthase kinase-3-like MsK-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.2 bits (2138), Expect = 3.2e-236
Identity = 402/409 (98.29%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SVSVVPASAI D GGSTIGV+RLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVSVVPASAIRDRGGSTIGVDRLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSL+YIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLSYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. NCBI nr
Match: KAG6606916.1 (Glycogen synthase kinase-3-like MsK-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 828.2 bits (2138), Expect = 3.2e-236
Identity = 402/409 (98.29%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SVSVVPASAI D GGSTIGV+RLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 32  MASVSVVPASAIRDRGGSTIGVDRLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 91

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 92  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 151

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 152 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 211

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSL+YIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 212 ICRSLSYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 271

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 272 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 331

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 332 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 391

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 392 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKHCNFLGS 440

BLAST of MS018950 vs. NCBI nr
Match: XP_038905663.1 (glycogen synthase kinase-3 homolog MsK-3 isoform X2 [Benincasa hispida])

HSP 1 Score: 826.6 bits (2134), Expect = 9.2e-236
Identity = 400/409 (97.80%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVPAS I DHGGSTIGV+RLPDEMNDMK+RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICR+L+YIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARK CNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKQCNFLGS 409

BLAST of MS018950 vs. ExPASy Swiss-Prot
Match: P51139 (Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=2 SV=2)

HSP 1 Score: 766.5 bits (1978), Expect = 1.5e-220
Identity = 367/410 (89.51%), Postives = 387/410 (94.39%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIE-ATVVDGNGTETGHIIVTT 60
           M S  V PAS   D   S++GVE+LP+EMNDMK+RDDKE+E AT+VDGNGTETGHIIVTT
Sbjct: 1   MASGGVAPASGFIDKNASSVGVEKLPEEMNDMKIRDDKEMEAATIVDGNGTETGHIIVTT 60

Query: 61  IGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
           IGGKNGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFY 180
           LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETV RVI+HYNKMNQRMP+IYVKLY Y
Sbjct: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVSRVIRHYNKMNQRMPMIYVKLYSY 180

Query: 181 QICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYY 240
           QICR+LAYIHNSIGVCHRDIKPQNLLVNPHTHQLK+CDFGSAKVLV+GEPNI+YICSRYY
Sbjct: 181 QICRALAYIHNSIGVCHRDIKPQNLLVNPHTHQLKICDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYT+AIDIWSAGCVL ELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTAIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDE 360
           CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEAL+HPF+D+
Sbjct: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALVHPFYDD 360

Query: 361 LRDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           +RDPNTRLPNGRFLPPLFNFK  ELKGVP EMLV+L+P HARK C   GS
Sbjct: 361 VRDPNTRLPNGRFLPPLFNFKVNELKGVPAEMLVKLVPPHARKQCALFGS 410

BLAST of MS018950 vs. ExPASy Swiss-Prot
Match: P43288 (Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1 SV=3)

HSP 1 Score: 748.4 bits (1931), Expect = 4.2e-215
Identity = 358/408 (87.75%), Postives = 381/408 (93.38%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P     D    + GV++LP+EMNDMK+RDDKE+EATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVGIAPNPGARD----STGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPLIYVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I R+L+YIH  IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRS AL+ L+HPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLG 409
           RDPN RLPNGRFLPPLFNFK  ELKGVP EM+ +L+PEHARK C +LG
Sbjct: 361 RDPNARLPNGRFLPPLFNFKPHELKGVPLEMVAKLVPEHARKQCPWLG 404

BLAST of MS018950 vs. ExPASy Swiss-Prot
Match: P43289 (Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 3.0e-213
Identity = 351/407 (86.24%), Postives = 381/407 (93.61%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P++A+ +  G+    +RLP+EM DMK++DDKE+EAT+V+GN TETGHIIVTTI
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I RSL+YIH  IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR  AL++L+HPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFL 408
           RDPN RLPNGRFLPPLFNFK  ELKGVP EM+ +L+PEHARK C +L
Sbjct: 361 RDPNARLPNGRFLPPLFNFKPHELKGVPVEMVAKLVPEHARKQCPWL 407

BLAST of MS018950 vs. ExPASy Swiss-Prot
Match: P51138 (Glycogen synthase kinase-3 homolog MsK-2 OS=Medicago sativa OX=3879 GN=MSK-2 PE=2 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 5.1e-213
Identity = 358/410 (87.32%), Postives = 379/410 (92.44%), Query Frame = 0

Query: 1   MTSVSVVPASAIPD-HGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTT 60
           M +  V PAS I D +  S I V++LPDE+  M+++DDKE+EA VVDGN TE GH+IVTT
Sbjct: 1   MATAGVAPASGIVDVNASSAIAVDKLPDEILGMRIKDDKEMEAHVVDGNSTEAGHVIVTT 60

Query: 61  IGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
           IGGKNGQPKQTISYMAERAVG GSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERAVGQGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFY 180
           LLDHPNVV+LKHCFFSTTEKDELYLNLVLE+VPETVHRVI+HY+KMNQRMPLIYVKLY Y
Sbjct: 121 LLDHPNVVTLKHCFFSTTEKDELYLNLVLEFVPETVHRVIRHYSKMNQRMPLIYVKLYSY 180

Query: 181 QICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYY 240
           QICRSLAYIHN +GV HRDIKPQNLLVNPHTHQLKLCDFGSAKVLV+GEPNI+YICSRYY
Sbjct: 181 QICRSLAYIHNCVGVSHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYTSAIDIWSAGCVL ELLLGQPLFPG SGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTSAIDIWSAGCVLGELLLGQPLFPGASGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDE 360
           CMNPNYTEFKFPQIKAHPWHKIF KRMPPEAVDLVSRLLQYSPNLRSTALEAL+HPFFDE
Sbjct: 301 CMNPNYTEFKFPQIKAHPWHKIFRKRMPPEAVDLVSRLLQYSPNLRSTALEALVHPFFDE 360

Query: 361 LRDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           LRDPNTRLPNGR LPPLFNFKA ELKGVP EMLV+L+P HARK C+   S
Sbjct: 361 LRDPNTRLPNGRHLPPLFNFKANELKGVPAEMLVKLVPSHARKQCSLFAS 410

BLAST of MS018950 vs. ExPASy Swiss-Prot
Match: Q40518 (Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 1.5e-212
Identity = 354/408 (86.76%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSV + P S + +    ++GV+RLP+EMNDM++RDDKEIEA +VDGNGTETGHIIVTTI
Sbjct: 1   MTSVGLAPVSGLRESSSHSVGVDRLPEEMNDMRIRDDKEIEAAIVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG++GQPKQTISYMAER VG GSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGRHGQPKQTISYMAERIVGQGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVV LKHCFFSTTEKDE+YLNLVLEYVPETVHRVIKHYNK+NQRMPLI VKLY YQ
Sbjct: 121 LDHPNVVCLKHCFFSTTEKDEVYLNLVLEYVPETVHRVIKHYNKLNQRMPLILVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I R+L+YIH++IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRALSYIHHTIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNY EFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR TALEA+ H FFDEL
Sbjct: 301 MNPNYNEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEAVTHAFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLG 409
           RDPNTRLPNGR LPPLFNFKA ELKGV  E L++L+PEHARK C  LG
Sbjct: 361 RDPNTRLPNGRVLPPLFNFKAHELKGVSAENLLKLVPEHARKQCPSLG 408

BLAST of MS018950 vs. ExPASy TrEMBL
Match: A0A6J1DLW7 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111022381 PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.0e-240
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. ExPASy TrEMBL
Match: A0A6J1FAG2 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111442293 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.2e-236
Identity = 401/409 (98.04%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVP S I DHGGSTIGV+RLPDEMNDM++RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARKHCNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. ExPASy TrEMBL
Match: A0A6J1J277 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111482043 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 9.9e-236
Identity = 399/409 (97.56%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSVVP S I DHGGSTIGV+RLPDEMNDM++RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVVPDSVIRDHGGSTIGVDRLPDEMNDMRIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDP+TRLPNGRFLPPLFNFKA ELKGVPPEML+RLIPEHARKHCNFLGS
Sbjct: 361 RDPSTRLPNGRFLPPLFNFKAHELKGVPPEMLMRLIPEHARKHCNFLGS 409

BLAST of MS018950 vs. ExPASy TrEMBL
Match: A0A5D3DAA6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00760 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 4.9e-235
Identity = 398/409 (97.31%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           MTSVSV PAS I DHGGSTIGV+RLPDEMNDMK+RDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MTSVSVAPASVIRDHGGSTIGVDRLPDEMNDMKIRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICR+L+YIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRALSYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFF+EL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFNEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLGS 410
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARK CNFLGS
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKQCNFLGS 409

BLAST of MS018950 vs. ExPASy TrEMBL
Match: A0A6J1GBR5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452723 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 6.4e-235
Identity = 399/408 (97.79%), Postives = 402/408 (98.53%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SVSVVPA  I D GGSTIGV+RLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVSVVPAPVIRDRGGSTIGVDRLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL
Sbjct: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           ICRSL+YIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR
Sbjct: 181 ICRSLSYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLG 409
           RDPNTRLPNGRFLPPLFNFKA ELKGVPPEMLVRLIPEHARKHCNFLG
Sbjct: 361 RDPNTRLPNGRFLPPLFNFKAHELKGVPPEMLVRLIPEHARKHCNFLG 408

BLAST of MS018950 vs. TAIR 10
Match: AT5G26751.1 (shaggy-related kinase 11 )

HSP 1 Score: 748.4 bits (1931), Expect = 3.0e-216
Identity = 358/408 (87.75%), Postives = 381/408 (93.38%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P     D    + GV++LP+EMNDMK+RDDKE+EATVVDGNGTETGHIIVTTI
Sbjct: 1   MASVGIAPNPGARD----STGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPLIYVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I R+L+YIH  IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRS AL+ L+HPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFLG 409
           RDPN RLPNGRFLPPLFNFK  ELKGVP EM+ +L+PEHARK C +LG
Sbjct: 361 RDPNARLPNGRFLPPLFNFKPHELKGVPLEMVAKLVPEHARKQCPWLG 404

BLAST of MS018950 vs. TAIR 10
Match: AT3G05840.2 (Protein kinase superfamily protein )

HSP 1 Score: 742.3 bits (1915), Expect = 2.1e-214
Identity = 351/407 (86.24%), Postives = 381/407 (93.61%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P++A+ +  G+    +RLP+EM DMK++DDKE+EAT+V+GN TETGHIIVTTI
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I RSL+YIH  IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR  AL++L+HPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFL 408
           RDPN RLPNGRFLPPLFNFK  ELKGVP EM+ +L+PEHARK C +L
Sbjct: 361 RDPNARLPNGRFLPPLFNFKPHELKGVPVEMVAKLVPEHARKQCPWL 407

BLAST of MS018950 vs. TAIR 10
Match: AT3G05840.1 (Protein kinase superfamily protein )

HSP 1 Score: 742.3 bits (1915), Expect = 2.1e-214
Identity = 351/407 (86.24%), Postives = 381/407 (93.61%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTTI 60
           M SV + P++A+ +  G+    +RLP+EM DMK++DDKE+EAT+V+GN TETGHIIVTTI
Sbjct: 1   MASVGIEPSAAVRESTGNVTDADRLPEEMKDMKIQDDKEMEATIVNGNVTETGHIIVTTI 60

Query: 61  GGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRL 120
           GG+NGQPKQTISYMAER VGHGSFGVVFQAKCLETGETVAIKKVLQD+RYKNRELQTMRL
Sbjct: 61  GGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRL 120

Query: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFYQ 180
           LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK+NQRMPL+YVKLY YQ
Sbjct: 121 LDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLVYVKLYTYQ 180

Query: 181 ICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYYR 240
           I RSL+YIH  IGVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYYR
Sbjct: 181 IFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYR 240

Query: 241 APELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300
           APELIFGATEYT+AID+WSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC
Sbjct: 241 APELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKC 300

Query: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDEL 360
           MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR  AL++L+HPFFDEL
Sbjct: 301 MNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCAALDSLVHPFFDEL 360

Query: 361 RDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFL 408
           RDPN RLPNGRFLPPLFNFK  ELKGVP EM+ +L+PEHARK C +L
Sbjct: 361 RDPNARLPNGRFLPPLFNFKPHELKGVPVEMVAKLVPEHARKQCPWL 407

BLAST of MS018950 vs. TAIR 10
Match: AT5G14640.1 (shaggy-like kinase 13 )

HSP 1 Score: 730.3 bits (1884), Expect = 8.4e-211
Identity = 349/408 (85.54%), Postives = 379/408 (92.89%), Query Frame = 0

Query: 1   MTSVSVVPASAI-PDHGGSTIGVERLPDEMNDMKLRDDKEIEATVVDGNGTETGHIIVTT 60
           M SV  +PAS++      ++I  E+LP+ +N+MK++DDKE+EA VVDGNGTETGHIIVTT
Sbjct: 1   MASVGTLPASSMATKQSNASICAEKLPEGINEMKIKDDKEMEAAVVDGNGTETGHIIVTT 60

Query: 61  IGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120
           IGGKNGQPKQTISYMAER VG GSFG+VFQAKCLETGETVAIKKVLQDKRYKNRELQTMR
Sbjct: 61  IGGKNGQPKQTISYMAERIVGQGSFGIVFQAKCLETGETVAIKKVLQDKRYKNRELQTMR 120

Query: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIYVKLYFY 180
           LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETV+RV KHY++ NQRMP+IYVKLY Y
Sbjct: 121 LLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVYRVSKHYSRANQRMPIIYVKLYTY 180

Query: 181 QICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAYICSRYY 240
           QICR+LAYIH  +GVCHRDIKPQNLLVNPHTHQ+KLCDFGSAKVLV+GEPNI+YICSRYY
Sbjct: 181 QICRALAYIHGGVGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYY 240

Query: 241 RAPELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300
           RAPELIFGATEYT+ IDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK
Sbjct: 241 RAPELIFGATEYTTTIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIK 300

Query: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALIHPFFDE 360
           CMNPNYTEFKFPQIKAHPWHKIFHKR PPEAVDLVSRLLQYSPNLRSTA+EA++HPFFDE
Sbjct: 301 CMNPNYTEFKFPQIKAHPWHKIFHKRTPPEAVDLVSRLLQYSPNLRSTAMEAIVHPFFDE 360

Query: 361 LRDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHARKHCNFL 408
           LRDPNTRLPNGR LPPLFNFK QELKG   E+L +LIP+HARK C+FL
Sbjct: 361 LRDPNTRLPNGRALPPLFNFKPQELKGASLELLSKLIPDHARKQCSFL 408

BLAST of MS018950 vs. TAIR 10
Match: AT4G00720.1 (shaggy-like protein kinase 32 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.0e-184
Identity = 310/408 (75.98%), Postives = 349/408 (85.54%), Query Frame = 0

Query: 1   MTSVSVVPASAIPDHGGSTIGVERLPDEMNDMKLRD-------DKEIEATVVDGNGTETG 60
           ++  SV   S +P      +  ++LPD M +MK+RD       DK++E TVV+G+GTETG
Sbjct: 60  LSQESVAGTSNVPAVSEKPVD-DQLPDVMIEMKIRDERNANREDKDMETTVVNGSGTETG 119

Query: 61  HIIVTTIGGKNGQPKQTISYMAERAVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNR 120
            +I TT+GG++G+PKQTISYMA+R VG GSFGVVFQAKCLETGE VAIKKVLQDKRYKNR
Sbjct: 120 QVITTTVGGRDGKPKQTISYMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNR 179

Query: 121 ELQTMRLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKMNQRMPLIY 180
           ELQ MRL DHPNVV L+H FFSTT+KDELYLNLVLEYVPETV+R  KHY KMNQ MP+I+
Sbjct: 180 ELQIMRLQDHPNVVRLRHSFFSTTDKDELYLNLVLEYVPETVYRASKHYTKMNQHMPIIF 239

Query: 181 VKLYFYQICRSLAYIHNSIGVCHRDIKPQNLLVNPHTHQLKLCDFGSAKVLVRGEPNIAY 240
           V+LY YQICR+L Y+H  +GVCHRDIKPQNLLVNP THQLK+CDFGSAK+LV GEPNI+Y
Sbjct: 240 VQLYTYQICRALNYLHRVVGVCHRDIKPQNLLVNPQTHQLKICDFGSAKMLVPGEPNISY 299

Query: 241 ICSRYYRAPELIFGATEYTSAIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTP 300
           ICSRYYRAPELIFGATEYT+AID+WS GCV+AELLLGQPLFPGESG+DQLVEIIK+LGTP
Sbjct: 300 ICSRYYRAPELIFGATEYTNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTP 359

Query: 301 TREEIKCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSTALEALI 360
           TREEI+CMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLR TALEA  
Sbjct: 360 TREEIRCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACA 419

Query: 361 HPFFDELRDPNTRLPNGRFLPPLFNFKAQELKGVPPEMLVRLIPEHAR 402
           HPFFD+LRDPN  LPNGR LPPLFNF AQEL G   E+  RLIP H +
Sbjct: 420 HPFFDDLRDPNVSLPNGRALPPLFNFTAQELAGASTELRQRLIPAHCQ 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155240.12.1e-240100.00glycogen synthase kinase-3 homolog MsK-3 [Momordica charantia][more]
XP_022935403.12.4e-23698.04glycogen synthase kinase-3 homolog MsK-3-like [Cucurbita moschata] >XP_023528441... [more]
XP_023523975.13.2e-23698.29glycogen synthase kinase-3 homolog MsK-3 [Cucurbita pepo subsp. pepo] >KAG703662... [more]
KAG6606916.13.2e-23698.29Glycogen synthase kinase-3-like MsK-3, partial [Cucurbita argyrosperma subsp. so... [more]
XP_038905663.19.2e-23697.80glycogen synthase kinase-3 homolog MsK-3 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P511391.5e-22089.51Glycogen synthase kinase-3 homolog MsK-3 OS=Medicago sativa OX=3879 GN=MSK-3 PE=... [more]
P432884.2e-21587.75Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1... [more]
P432893.0e-21386.24Shaggy-related protein kinase gamma OS=Arabidopsis thaliana OX=3702 GN=ASK3 PE=1... [more]
P511385.1e-21387.32Glycogen synthase kinase-3 homolog MsK-2 OS=Medicago sativa OX=3879 GN=MSK-2 PE=... [more]
Q405181.5e-21286.76Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1DLW71.0e-240100.00Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1FAG21.2e-23698.04Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J2779.9e-23697.56Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5D3DAA64.9e-23597.31Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1GBR56.4e-23597.79Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G26751.13.0e-21687.75shaggy-related kinase 11 [more]
AT3G05840.22.1e-21486.24Protein kinase superfamily protein [more]
AT3G05840.12.1e-21486.24Protein kinase superfamily protein [more]
AT5G14640.18.4e-21185.54shaggy-like kinase 13 [more]
AT4G00720.11.0e-18475.98shaggy-like protein kinase 32 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 73..357
e-value: 3.6E-87
score: 305.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 73..357
e-value: 3.8E-64
score: 216.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 73..357
score: 44.244381
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 57..152
e-value: 1.2E-35
score: 123.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 153..401
e-value: 2.6E-81
score: 274.6
NoneNo IPR availablePANTHERPTHR24057GLYCOGEN SYNTHASE KINASE-3 ALPHAcoord: 1..407
NoneNo IPR availablePANTHERPTHR24057:SF65SHAGGY-RELATED PROTEIN KINASE ALPHAcoord: 1..407
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 79..103
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 194..206
IPR039192Glycogen synthase kinase 3, catalytic domainCDDcd14137STKc_GSK3coord: 68..360
e-value: 0.0
score: 601.799
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 72..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018950.1MS018950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity