MS018898 (gene) Bitter gourd (TR) v1

Overview


Name	MS018898
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	calmodulin-like
Location	scaffold89: 267143 .. 267385 (+)
RNA-Seq Expression	MS018898
Synteny	MS018898

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAGCAGGCTACACTTCTCCAGGGAGGAGATGAAGGAGATTTTCAGAGAGCACGACCTCGACGGAGATGGTCGATTGAGCATCAGCGAGCTCGTAAAGGCTTTCGGGTTCCTCGGTAACATCCTCCCTTTCTACAAAGCTCATTACGGTTTGGCTCATGCTGATGCTGATGAAGATGGCTTCATTTGTGAGGACGAGCTCGATAAGCTCGTCAACTACGCGGAGAAGTTTCAGCCCAAGCAG

mRNA sequence

GCAAGCAGGCTACACTTCTCCAGGGAGGAGATGAAGGAGATTTTCAGAGAGCACGACCTCGACGGAGATGGTCGATTGAGCATCAGCGAGCTCGTAAAGGCTTTCGGGTTCCTCGGTAACATCCTCCCTTTCTACAAAGCTCATTACGGTTTGGCTCATGCTGATGCTGATGAAGATGGCTTCATTTGTGAGGACGAGCTCGATAAGCTCGTCAACTACGCGGAGAAGTTTCAGCCCAAGCAG

Coding sequence (CDS)

GCAAGCAGGCTACACTTCTCCAGGGAGGAGATGAAGGAGATTTTCAGAGAGCACGACCTCGACGGAGATGGTCGATTGAGCATCAGCGAGCTCGTAAAGGCTTTCGGGTTCCTCGGTAACATCCTCCCTTTCTACAAAGCTCATTACGGTTTGGCTCATGCTGATGCTGATGAAGATGGCTTCATTTGTGAGGACGAGCTCGATAAGCTCGTCAACTACGCGGAGAAGTTTCAGCCCAAGCAG

Protein sequence

ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDELDKLVNYAEKFQPKQ

Homology

[Close]

BLAST of MS018898 vs. NCBI nr
Match: KAA0060892.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 7.4e-28
Identity = 61/81 (75.31%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1  ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
          AS+L  SRE++KEIFREHD+DGDGRLS+SE+VKAFGF G+ILPFYKAHYG+AHAD + DG
Sbjct: 5  ASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADENGDG 64

Query: 61 FICEDELDKLVNYAEKFQPKQ 82
          +I E+ELDKLV+YAEKFQ K+
Sbjct: 65 YIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of MS018898 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 129.0 bits (323), Expect = 1.8e-26
Identity = 59/81 (72.84%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1  ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
          A+RL  SRE++KEIFREHD+DGDGRLS++E+VKAFG  G+ILPFYKAHYG+AHAD + DG
Sbjct: 5  ATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 64

Query: 61 FICEDELDKLVNYAEKFQPKQ 82
          FI E+ELDKLV+YA+KFQ K+
Sbjct: 65 FIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of MS018898 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 127.9 bits (320), Expect = 4.0e-26
Identity = 58/81 (71.60%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1   ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
           ++RL  SRE++KEIFREHD+DGDGRLS++E+VKAFG  G+ILPFYKAHYG+AHAD + DG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 61  FICEDELDKLVNYAEKFQPKQ 82
           FI E+ELDKLV+YA+KFQ K+
Sbjct: 146 FIEEEELDKLVDYAQKFQHKR 166

[Close]

BLAST of MS018898 vs. NCBI nr
Match: KGN62525.1 (hypothetical protein Csa_022453 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 2.0e-25
Identity = 56/80 (70.00%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 2  SRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGF 61
          S+ +FSREEMKEIFREHD+DGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 62 ICEDELDKLVNYAEKFQPKQ 82
          I EDELDKL++YAE+FQ ++
Sbjct: 67 ISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of MS018898 vs. NCBI nr
Match: KAA0060886.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 119.4 bits (298), Expect = 1.4e-23
Identity = 53/75 (70.67%), Postives = 66/75 (88.00%), Query Frame = 0


Query: 2  SRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGF 61
          S+ +FSREEMKEIFREHD+DGDG LSI EL+KA GFLG+ +PFYKAHYG+A++D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSDENGDGL 66

Query: 62 ICEDELDKLVNYAEK 77
          I EDELDKL++YAE+
Sbjct: 67 ISEDELDKLIDYAER 81

[Close]

BLAST of MS018898 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.7e-06
Identity = 29/62 (46.77%), Postives = 37/62 (59.68%), Query Frame = 0


Query: 9   EEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDELD 68
           EEM+ +FR+ D +GDGR+S SEL   F  LG+     +    +A ADAD DGFI  DE  
Sbjct: 39  EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFA 98

Query: 69  KL 71
            L
Sbjct: 99  AL 100

[Close]

BLAST of MS018898 vs. ExPASy Swiss-Prot
Match: Q9NZT1 (Calmodulin-like protein 5 OS=Homo sapiens OX=9606 GN=CALML5 PE=1 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 3.5e-04
Identity = 22/71 (30.99%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 1   ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
           A +     E+++  FR  D DGDG +++ EL +A   LG  LP  +    +  AD D+DG
Sbjct: 73  AKKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDG 132

Query: 61  FICEDELDKLV 72
            +  +E  +++
Sbjct: 133 RVNYEEFARML 143

[Close]

BLAST of MS018898 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 5.9e-04
Identity = 20/54 (37.04%), Postives = 30/54 (55.56%), Query Frame = 0


Query: 7   SREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 61
           S+EE++++F   D DGDG++S  EL   FG +G  +    A   +   D D DG
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADG 114

[Close]

BLAST of MS018898 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 7.7e-04
Identity = 22/62 (35.48%), Postives = 34/62 (54.84%), Query Frame = 0


Query: 9  EEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDELD 68
          EE+K++F + D +GDG++S+ EL   F  +G      + +  L   D D DG+I  DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 69 KL 71
           L
Sbjct: 82 TL 83

[Close]

BLAST of MS018898 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.6e-28
Identity = 61/81 (75.31%), Postives = 74/81 (91.36%), Query Frame = 0


Query: 1  ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
          AS+L  SRE++KEIFREHD+DGDGRLS+SE+VKAFGF G+ILPFYKAHYG+AHAD + DG
Sbjct: 5  ASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADENGDG 64

Query: 61 FICEDELDKLVNYAEKFQPKQ 82
          +I E+ELDKLV+YAEKFQ K+
Sbjct: 65 YIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of MS018898 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.8e-27
Identity = 59/81 (72.84%), Postives = 73/81 (90.12%), Query Frame = 0


Query: 1  ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
          A+RL  SRE++KEIFREHD+DGDGRLS++E+VKAFG  G+ILPFYKAHYG+AHAD + DG
Sbjct: 5  ATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 64

Query: 61 FICEDELDKLVNYAEKFQPKQ 82
          FI E+ELDKLV+YA+KFQ K+
Sbjct: 65 FIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of MS018898 vs. ExPASy TrEMBL
Match: A0A0A0LKX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.7e-26
Identity = 56/80 (70.00%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 2  SRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGF 61
          S+ +FSREEMKEIFREHD+DGDG LSISEL+KA GFLG+ +PFYKAHYG+A+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 62 ICEDELDKLVNYAEKFQPKQ 82
          I EDELDKL++YAE+FQ ++
Sbjct: 67 ISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of MS018898 vs. ExPASy TrEMBL
Match: A0A5A7V2Z9 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00190 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.0e-24
Identity = 53/75 (70.67%), Postives = 66/75 (88.00%), Query Frame = 0


Query: 2  SRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGF 61
          S+ +FSREEMKEIFREHD+DGDG LSI EL+KA GFLG+ +PFYKAHYG+A++D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSDENGDGL 66

Query: 62 ICEDELDKLVNYAEK 77
          I EDELDKL++YAE+
Sbjct: 67 ISEDELDKLIDYAER 81

[Close]

BLAST of MS018898 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-22
Identity = 52/76 (68.42%), Postives = 64/76 (84.21%), Query Frame = 0


Query: 1   ASRLHFSREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 60
           A ++ FSR ++KEIF EHD+DGDG LS+SEL+KAF FLG+++P YKAHYGLA AD D DG
Sbjct: 68  APKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDG 127

Query: 61  FICEDELDKLVNYAEK 77
            I E+ELDKLV+YAEK
Sbjct: 128 LISEEELDKLVDYAEK 143

[Close]

BLAST of MS018898 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 44.7 bits (104), Expect = 4.2e-05
Identity = 20/54 (37.04%), Postives = 30/54 (55.56%), Query Frame = 0


Query: 7   SREEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDG 61
           S+EE++++F   D DGDG++S  EL   FG +G  +    A   +   D D DG
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADG 114

[Close]

BLAST of MS018898 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.3 bits (103), Expect = 5.5e-05
Identity = 22/62 (35.48%), Postives = 34/62 (54.84%), Query Frame = 0


Query: 9  EEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDELD 68
          EE+K++F + D +GDG++S+ EL   F  +G      + +  L   D D DG+I  DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 69 KL 71
           L
Sbjct: 82 TL 83

[Close]

BLAST of MS018898 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.3 bits (103), Expect = 5.5e-05
Identity = 22/62 (35.48%), Postives = 34/62 (54.84%), Query Frame = 0


Query: 9  EEMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDELD 68
          EE+K++F + D +GDG++S+ EL   F  +G      + +  L   D D DG+I  DE  
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 69 KL 71
           L
Sbjct: 82 TL 83

[Close]

BLAST of MS018898 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.1 bits (100), Expect = 1.2e-04
Identity = 20/64 (31.25%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 9   EEMKEIFREHDLDGDGRLSISELVKAFGFLGN-ILPFYKAHYGLAHADADEDGFICEDEL 68
           EE++E+F   D+D +G++S  EL + FG +G+      +    +A  D + DGF+C D+ 
Sbjct: 144 EELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRMIATVDGNGDGFVCFDDF 203

Query: 69  DKLV 72
            +++
Sbjct: 204 CRMM 207

[Close]

BLAST of MS018898 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 42.7 bits (99), Expect = 1.6e-04
Identity = 21/57 (36.84%), Postives = 34/57 (59.65%), Query Frame = 0


Query: 10 EMKEIFREHDLDGDGRLSISELVKAFGFLGNILPFYKAHYGLAHADADEDGFICEDE 67
          E+K++F + D +GDG++S+SEL   F  +G      + +  L   D D DGFI ++E
Sbjct: 20 ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEE 76

The following BLAST results are available for this feature:

BLAST of MS018898 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0060892.1	7.4e-28	75.31	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KGN62516.1	1.8e-26	72.84	hypothetical protein Csa_022521 [Cucumis sativus]	[more]
XP_004152805.1	4.0e-26	71.60	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
KGN62525.1	2.0e-25	70.00	hypothetical protein Csa_022453 [Cucumis sativus]	[more]
KAA0060886.1	1.4e-23	70.67	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]

BLAST of MS018898 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	3.7e-06	46.77	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9NZT1	3.5e-04	30.99	Calmodulin-like protein 5 OS=Homo sapiens OX=9606 GN=CALML5 PE=1 SV=2	[more]
Q8L3R2	5.9e-04	37.04	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9LE22	7.7e-04	35.48	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]

BLAST of MS018898 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYC0	3.6e-28	75.31	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LRC1	8.8e-27	72.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1	9.7e-26	70.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1	[more]
A0A5A7V2Z9	7.0e-24	70.67	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CSH1	1.3e-22	68.42	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of MS018898 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	4.2e-05	37.04	calmodulin-like 41	[more]
AT1G18210.1	5.5e-05	35.48	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	5.5e-05	35.48	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	1.2e-04	31.25	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	1.6e-04	36.84	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 10..38 e-value: 0.005 score: 26.1
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 54..71 e-value: 0.028 score: 13.9
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 10..38 e-value: 1.4E-7 score: 30.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 6..41 score: 14.234451
None	No IPR available	GENE3D	1.10.238.10		coord: 5..77 e-value: 3.0E-11 score: 44.7
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 19..31
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 55..67
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018898.1	MS018898.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding