MS018877 (gene) Bitter gourd (TR) v1

Overview
NameMS018877
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionheat stress transcription factor B-4
Locationscaffold89: 102275 .. 103397 (+)
RNA-Seq ExpressionMS018877
SyntenyMS018877
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATCTGTAATGGTGGAGAATTGCGACGGCAGCCTCCTGCTGTCCCTAGACTCCCAGAAGTCCGTCCCGGCGCCGTTCCTCTCCAAAACGTACCAGCTGGTGGACGACCCCTCCACCGACCACATCGTCTCCTGGGGCCAAGACCTCTCCACCTTCGTCGTGTGGCGCCCCCCCGAGTTCGCCCGCGACCTCCTCCCCAATTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTACGTCCGATCCGTTCCCTACACTTTATTTAAAATTTCACATTCTAATTCCTCCTTCTTCAAAAATTTTCATTTTGTTTTTTCATTTTGTTTTATAGGGGTTCCGCAAGATTGTTCCCGACAGATGGGAGTTCGCCAACGAGTTCTTCCGCAAGGGGGAGAAGCATTTACTCTGCGAGATCCACAGACGTAAAACCGCCCACCCCCAAGTCTCCTACAGTACCGGCCCCACTACTTTCTTCCCCCGCCTCAGCATCTCCCCCTCCGACTCCGACGACCAACTTCCCAACTGGCTGTGCGACTCCCCCCCGCCGCCGCTCCCCACCACCACCACCAACTACAACATCATCGGCATCGGCATCGGCTCCGTCGCCGCTTTGTCCCAAGACAACGACCGCTTGAGGAGGAATAACTGTATGCTCCTTTCCGAGCTTGCCCACATGAGGAAACTCTACAATGATATTATCTATTTTGTTCAGAACCATGTCAAGCCTGTCTCACCTCCTCCCACCACCAATTCTTTCCTCCTTTCTCACCCAGCTCCCACTCATAATTCTTCCCAAAAACCCCTCAATCACTTTCTTGGATTCTGCCCTAACAAGCACACTTCTCCTGGCACTGCTGCTACGGCCCACTTTCTTGATTCTCCGACTGCAACGTCGAAGAGTGCGATTACGGAGCTCGACGCATTTGACAACAACAGCAACACATGCAGGACCAAGCTGTTTGGAGTGCCGCTTCCGTTGTCTAAGAAAAGGCTGCACCCTGATACTACTTATGGTTCTATTTCCTCTTCCAACAAGAGTAGTTCTTCTCGCTTGGTCTTGGAAAAAGGTGACAATTTGGGCCTCAATCTTATGCCTCCTTCTAATAGC

mRNA sequence

TCATCTGTAATGGTGGAGAATTGCGACGGCAGCCTCCTGCTGTCCCTAGACTCCCAGAAGTCCGTCCCGGCGCCGTTCCTCTCCAAAACGTACCAGCTGGTGGACGACCCCTCCACCGACCACATCGTCTCCTGGGGCCAAGACCTCTCCACCTTCGTCGTGTGGCGCCCCCCCGAGTTCGCCCGCGACCTCCTCCCCAATTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTACGGGTTCCGCAAGATTGTTCCCGACAGATGGGAGTTCGCCAACGAGTTCTTCCGCAAGGGGGAGAAGCATTTACTCTGCGAGATCCACAGACGTAAAACCGCCCACCCCCAAGTCTCCTACAGTACCGGCCCCACTACTTTCTTCCCCCGCCTCAGCATCTCCCCCTCCGACTCCGACGACCAACTTCCCAACTGGCTGTGCGACTCCCCCCCGCCGCCGCTCCCCACCACCACCACCAACTACAACATCATCGGCATCGGCATCGGCTCCGTCGCCGCTTTGTCCCAAGACAACGACCGCTTGAGGAGGAATAACTGTATGCTCCTTTCCGAGCTTGCCCACATGAGGAAACTCTACAATGATATTATCTATTTTGTTCAGAACCATGTCAAGCCTGTCTCACCTCCTCCCACCACCAATTCTTTCCTCCTTTCTCACCCAGCTCCCACTCATAATTCTTCCCAAAAACCCCTCAATCACTTTCTTGGATTCTGCCCTAACAAGCACACTTCTCCTGGCACTGCTGCTACGGCCCACTTTCTTGATTCTCCGACTGCAACGTCGAAGAGTGCGATTACGGAGCTCGACGCATTTGACAACAACAGCAACACATGCAGGACCAAGCTGTTTGGAGTGCCGCTTCCGTTGTCTAAGAAAAGGCTGCACCCTGATACTACTTATGGTTCTATTTCCTCTTCCAACAAGAGTAGTTCTTCTCGCTTGGTCTTGGAAAAAGGTGACAATTTGGGCCTCAATCTTATGCCTCCTTCTAATAGC

Coding sequence (CDS)

TCATCTGTAATGGTGGAGAATTGCGACGGCAGCCTCCTGCTGTCCCTAGACTCCCAGAAGTCCGTCCCGGCGCCGTTCCTCTCCAAAACGTACCAGCTGGTGGACGACCCCTCCACCGACCACATCGTCTCCTGGGGCCAAGACCTCTCCACCTTCGTCGTGTGGCGCCCCCCCGAGTTCGCCCGCGACCTCCTCCCCAATTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTACGGGTTCCGCAAGATTGTTCCCGACAGATGGGAGTTCGCCAACGAGTTCTTCCGCAAGGGGGAGAAGCATTTACTCTGCGAGATCCACAGACGTAAAACCGCCCACCCCCAAGTCTCCTACAGTACCGGCCCCACTACTTTCTTCCCCCGCCTCAGCATCTCCCCCTCCGACTCCGACGACCAACTTCCCAACTGGCTGTGCGACTCCCCCCCGCCGCCGCTCCCCACCACCACCACCAACTACAACATCATCGGCATCGGCATCGGCTCCGTCGCCGCTTTGTCCCAAGACAACGACCGCTTGAGGAGGAATAACTGTATGCTCCTTTCCGAGCTTGCCCACATGAGGAAACTCTACAATGATATTATCTATTTTGTTCAGAACCATGTCAAGCCTGTCTCACCTCCTCCCACCACCAATTCTTTCCTCCTTTCTCACCCAGCTCCCACTCATAATTCTTCCCAAAAACCCCTCAATCACTTTCTTGGATTCTGCCCTAACAAGCACACTTCTCCTGGCACTGCTGCTACGGCCCACTTTCTTGATTCTCCGACTGCAACGTCGAAGAGTGCGATTACGGAGCTCGACGCATTTGACAACAACAGCAACACATGCAGGACCAAGCTGTTTGGAGTGCCGCTTCCGTTGTCTAAGAAAAGGCTGCACCCTGATACTACTTATGGTTCTATTTCCTCTTCCAACAAGAGTAGTTCTTCTCGCTTGGTCTTGGAAAAAGGTGACAATTTGGGCCTCAATCTTATGCCTCCTTCTAATAGC

Protein sequence

SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS
Homology
BLAST of MS018877 vs. NCBI nr
Match: XP_022144161.1 (heat stress transcription factor B-4 [Momordica charantia])

HSP 1 Score: 699.5 bits (1804), Expect = 1.4e-197
Identity = 340/343 (99.13%), Postives = 340/343 (99.13%), Query Frame = 0

Query: 1   SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF 60
           SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF
Sbjct: 2   SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF 61

Query: 61  ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP 120
           ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP
Sbjct: 62  ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP 121

Query: 121 QVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIGIGSVAALSQ 180
           QVSYSTGPTTFFPRLSISPSDSDDQLPNW CDSPPPPLPTTTTNYNIIGIGIGSVA LSQ
Sbjct: 122 QVSYSTGPTTFFPRLSISPSDSDDQLPNWPCDSPPPPLPTTTTNYNIIGIGIGSVATLSQ 181

Query: 181 DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK 240
           DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK
Sbjct: 182 DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK 241

Query: 241 PLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL 300
           PLNHFLGFCPNKHTSPGT ATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL
Sbjct: 242 PLNHFLGFCPNKHTSPGTVATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL 301

Query: 301 SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS 344
           SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS
Sbjct: 302 SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS 344

BLAST of MS018877 vs. NCBI nr
Match: XP_038885667.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 477.6 bits (1228), Expect = 8.8e-131
Identity = 256/379 (67.55%), Postives = 291/379 (76.78%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIGI 181
           + YS          +FFP   R+SISPSDSD+Q+ NW CDSPP P     + Y+++G GI
Sbjct: 122 LPYSHHHLGNLNAPSFFPFSTRVSISPSDSDEQI-NW-CDSPPAPPKGGASGYSVMGSGI 181

Query: 182 -------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSP 241
                         SV ALS+DN+RLRRNN ML+SEL HMRKLYNDIIYFVQNHVKPVSP
Sbjct: 182 ISNNSSTSSYNMMSSVTALSEDNERLRRNNSMLISELTHMRKLYNDIIYFVQNHVKPVSP 241

Query: 242 PPTTNSFLLSHPAPTHNSSQKPLNHFLGFCP------------NKHTSPGTAATAHFLDS 301
             +  S LL HP+ T  +SQKPLNHFLG  P            N  + P T  T HF+ S
Sbjct: 242 SNSFPSLLLCHPSSTTPNSQKPLNHFLGSSPSTTHSNTATITTNHFSIPATIGTPHFVSS 301

Query: 302 PTATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT-YGSISS--SNKSSSSR 344
           PTATSKSA+TELD  + ++N+C+TKLFGVP+  SKKR+HPDTT Y SISS  S+ + +SR
Sbjct: 302 PTATSKSAVTELDVGEADNNSCKTKLFGVPISSSKKRMHPDTTNYVSISSSVSDTNKNSR 361

BLAST of MS018877 vs. NCBI nr
Match: XP_004143013.1 (heat stress transcription factor B-4 [Cucumis sativus] >KGN62495.1 hypothetical protein Csa_018602 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 8.8e-131
Identity = 260/378 (68.78%), Postives = 295/378 (78.04%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + Y+        GP +FFP   R+SISPSDSD+Q+ NW CDSPP PP  T T  Y+++G 
Sbjct: 122 LPYTHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPVPPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSSTSNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH  P+  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSH-HPSTTNSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. NCBI nr
Match: KAA0060911.1 (heat stress transcription factor B-4 [Cucumis melo var. makuwa])

HSP 1 Score: 475.7 bits (1223), Expect = 3.3e-130
Identity = 259/378 (68.52%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + YS        GP +FFP   R+SISPSDSD+Q+ NW CDSPP  P  T T  Y+++G 
Sbjct: 122 LPYSHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPLAPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSSASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH   +  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. NCBI nr
Match: XP_008444610.1 (PREDICTED: heat stress transcription factor B-4 [Cucumis melo])

HSP 1 Score: 475.7 bits (1223), Expect = 3.3e-130
Identity = 259/378 (68.52%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + YS        GP +FFP   R+SISPSDSD+Q+ NW CDSPP  P  T T  Y+++G 
Sbjct: 122 LPYSHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPVAPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSRASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH   +  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.4e-84
Identity = 191/369 (51.76%), Postives = 233/369 (63.14%), Query Frame = 0

Query: 2   SVMVENCDGS--------LLLSLDSQ-KSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTF 61
           ++MVEN  G         + L ++ Q K+VPAPFL+KTYQLVDDP+TDH+VSWG D +TF
Sbjct: 2   AMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTF 61

Query: 62  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEI 121
           VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCEI
Sbjct: 62  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEI 121

Query: 122 HRRKT----------------AHPQVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPP 181
           HRRKT                A PQ+ +S G  +FFP     P  +  +  ++ CD  PP
Sbjct: 122 HRRKTSQMIPQQHSPFMSHHHAPPQIPFSGG--SFFP--LPPPRVTTPEEDHYWCDDSPP 181

Query: 182 PLPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKP 241
             P               V ALS+DN+RLRR+N +L+SELAHM+KLYNDIIYFVQNHVKP
Sbjct: 182 SRPRVIPQQIDTA---AQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKP 241

Query: 242 VSPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAI 301
           V+  P+ NS  LS         Q P   +       +T+   A   + L+S   TS+S+I
Sbjct: 242 VA--PSNNSSYLSSFLQKQQQQQPPTLDYY------NTATVNATNLNALNSSPPTSQSSI 301

Query: 302 TELDAFDNN----SNTCRTKLFGVPLPLSKKRLHPDTTYGSISSSNKSSSSRLVLEKGDN 342
           T L+    N    SN  +TKLFGV LP SKKR H          S+++S +RLVL++ D 
Sbjct: 302 TVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH--------HFSDQTSKTRLVLDQSD- 346

BLAST of MS018877 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 2.0e-69
Identity = 161/331 (48.64%), Postives = 190/331 (57.40%), Query Frame = 0

Query: 4   MVENCDGSLLLSLD----------SQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFV 63
           +VE C G +++S++          + K VPAPFL+KTYQLVDDP TDHIVSWG+D +TFV
Sbjct: 4   LVERC-GEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTTFV 63

Query: 64  VWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIH 123
           VWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL EIH
Sbjct: 64  VWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAEIH 123

Query: 124 RRKTAHPQVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPPPL---------PTTTTN 183
           RRK++ P            P +   P      L  +    PPP           P TT +
Sbjct: 124 RRKSSQPPP----------PPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATTAH 183

Query: 184 YNIIGIGIGS---VAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPP 243
             + G G      +AALS+DN +LRR N +LLSELAHM+KLYNDIIYF+QNHV PV+   
Sbjct: 184 CTVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPVTTTT 243

Query: 244 TTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAITELDA 303
           TT S          +++     H        H     +     LDSP  +      +  A
Sbjct: 244 TTPS----------STAMAAAQH--------HLPAAASCRLMELDSPDHSPPPPPPKTPA 302

Query: 304 FDNNSNTCRTKLFGVPLPLSKKRLHPDTTYG 313
            D        KLFGV L   KKR H D   G
Sbjct: 304 TDGGDT---VKLFGVSLHGRKKRAHRDDDDG 302

BLAST of MS018877 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.5e-68
Identity = 139/244 (56.97%), Postives = 162/244 (66.39%), Query Frame = 0

Query: 4   MVENCDGSLLLSLD-----------SQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTF 63
           +VE C G +++S++           +  + PAPFLSKTYQLVDDPSTD +VSWG+D +TF
Sbjct: 4   LVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDEATF 63

Query: 64  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEI 123
           VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL EI
Sbjct: 64  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLSEI 123

Query: 124 HRRKT---AHPQVSYSTGPTTFFPRLSISPSDSDDQLP------------NWLCDSPPPP 183
           HRRK+   + PQ          +P    SP  +    P               C SP   
Sbjct: 124 HRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHFQEEYCSSPA-- 183

Query: 184 LPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 222
                   +  G G   +AALS+DN +LRR N +LLSELAHMRKLYNDIIYF+QNHV+PV
Sbjct: 184 --------DYAGGGGDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEPV 237

BLAST of MS018877 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.4e-68
Identity = 174/375 (46.40%), Postives = 211/375 (56.27%), Query Frame = 0

Query: 18  SQKSVPAPFLSKTYQLVDDPSTDHIVSWGQD-LSTFVVWRPPEFARDLLPNYFKHNNFSS 77
           +QK+VPAPFL+KTYQLVDDP+TDHIVSWG D +STFVVWRPPEFARD+LPNYFKHNNFSS
Sbjct: 17  AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSS 76

Query: 78  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQVSYSTGPTTFFP--- 137
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+    +  + P  F P   
Sbjct: 77  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPSPPPFFAPPHF 136

Query: 138 ----RLSISPSD------SDDQL------------PNW-----------LCDSPPPPLPT 197
                  ++ +        DD +            P+W           L    P P P+
Sbjct: 137 PLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLALGGPAPSPS 196

Query: 198 TTTNYNIIGIGIGSVAA-LSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSP 257
           +       G G  + AA L ++N+RLRR+N  LL ELAHMRKLYNDIIYFVQNHV+PV+P
Sbjct: 197 SA---EAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQNHVRPVAP 256

Query: 258 PPTTNSFL--LSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAIT 317
            P   +FL  L   A    ++   LN+  G      TS  +   A     P      A  
Sbjct: 257 SPAAAAFLQGLGMQARKKPAAANVLNNSGG----STTSSSSLTIAEEPSPPPQQQHLAGE 316

Query: 318 ELDAFDNNSNTCR----TKLFGV-----PLPLSKKRLHPDTTYGSISSSNKSSSSRLVLE 344
           +      NS+  R    TKLFGV     P     KR      +   S + K    RLVLE
Sbjct: 317 KSGGEAGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPEEHPPTSPATK---PRLVLE 376

BLAST of MS018877 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 150/321 (46.73%), Postives = 183/321 (57.01%), Query Frame = 0

Query: 18  SQKSVPAPFLSKTYQLVDDPSTDHIVSW-----GQDLSTFVVWRPPEFARDLLPNYFKHN 77
           S   VPAPFL+KTYQLVDDP+TDH+VSW     G+  S+FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 78  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ-VSYSTGPTTF 137
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A      +   P  F
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 138 F-PRLSISPS---DSDDQLPNWL------------CDSPPPPLPTTTTNYNIIGIGIGSV 197
           F PR   + +     D  L   L             +S   P+  ++   + +G  I   
Sbjct: 138 FAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPA 197

Query: 198 ---------AALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSF 257
                     AL Q+N RL R N  L+ ELAHMRKLY+DIIYFVQNHV+PV+P P   + 
Sbjct: 198 RRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPSPAAAAA 257

Query: 258 L--LSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPT-------ATSKSAIT 299
           L  L    P     + P +   G      TS  +   A   D PT        T++  I 
Sbjct: 258 LHGLGVLRPPPAGGKAPASEVRGASGRSATSSSSLTVAE--DQPTLLALRLPRTTEKIIN 317

BLAST of MS018877 vs. ExPASy TrEMBL
Match: A0A6J1CSH7 (heat stress transcription factor B-4 OS=Momordica charantia OX=3673 GN=LOC111013920 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 6.9e-198
Identity = 340/343 (99.13%), Postives = 340/343 (99.13%), Query Frame = 0

Query: 1   SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF 60
           SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF
Sbjct: 2   SSVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEF 61

Query: 61  ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP 120
           ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP
Sbjct: 62  ARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHP 121

Query: 121 QVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIGIGSVAALSQ 180
           QVSYSTGPTTFFPRLSISPSDSDDQLPNW CDSPPPPLPTTTTNYNIIGIGIGSVA LSQ
Sbjct: 122 QVSYSTGPTTFFPRLSISPSDSDDQLPNWPCDSPPPPLPTTTTNYNIIGIGIGSVATLSQ 181

Query: 181 DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK 240
           DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK
Sbjct: 182 DNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQK 241

Query: 241 PLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL 300
           PLNHFLGFCPNKHTSPGT ATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL
Sbjct: 242 PLNHFLGFCPNKHTSPGTVATAHFLDSPTATSKSAITELDAFDNNSNTCRTKLFGVPLPL 301

Query: 301 SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS 344
           SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS
Sbjct: 302 SKKRLHPDTTYGSISSSNKSSSSRLVLEKGDNLGLNLMPPSNS 344

BLAST of MS018877 vs. ExPASy TrEMBL
Match: A0A0A0LKU2 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 4.3e-131
Identity = 260/378 (68.78%), Postives = 295/378 (78.04%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + Y+        GP +FFP   R+SISPSDSD+Q+ NW CDSPP PP  T T  Y+++G 
Sbjct: 122 LPYTHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPVPPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSSTSNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH  P+  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSH-HPSTTNSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. ExPASy TrEMBL
Match: A0A5A7V551 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00440 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 259/378 (68.52%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + YS        GP +FFP   R+SISPSDSD+Q+ NW CDSPP  P  T T  Y+++G 
Sbjct: 122 LPYSHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPLAPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSSASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH   +  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. ExPASy TrEMBL
Match: A0A1S3BAP2 (heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 259/378 (68.52%), Postives = 293/378 (77.51%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           SVM++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SVMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYS-------TGPTTFFP---RLSISPSDSDDQLPNWLCDSPP-PPLPTTTTNYNIIGI 181
           + YS        GP +FFP   R+SISPSDSD+Q+ NW CDSPP  P  T T  Y+++G 
Sbjct: 122 LPYSHHHLGNINGP-SFFPFSTRVSISPSDSDEQI-NW-CDSPPVAPRATGTGGYSVMGS 181

Query: 182 GI-------------GSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPV 241
           GI              SV ALS+DN+RLRRNN ML+SEL+HMRKLYNDIIYFVQNHVKPV
Sbjct: 182 GIISNNSRASNYNMMSSVTALSEDNERLRRNNSMLISELSHMRKLYNDIIYFVQNHVKPV 241

Query: 242 SPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTS--------PGTAATAHFLDSPT 301
           SP  +  S LLSH   +  +SQKPLNHFLG  P   TS        PGT  T HF+ SPT
Sbjct: 242 SPSNSFPSLLLSHHPSSTANSQKPLNHFLGISPPNTTSTTTNHFSVPGTIVTTHFVSSPT 301

Query: 302 ATSKSAITELDAFDNNSNTCRTKLFGVPLPLSKKRLHPDTT--YGSISS--SNKSSSSRL 344
           ATSKSA+TELD  + ++N+C+TKLFGV +  SKKR+HPDTT  Y SI S  S+ + +SRL
Sbjct: 302 ATSKSAVTELDVGEADNNSCKTKLFGVAISSSKKRMHPDTTTNYVSICSNVSDTNKNSRL 361

BLAST of MS018877 vs. ExPASy TrEMBL
Match: A0A6J1KEA4 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111492453 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 9.2e-126
Identity = 252/383 (65.80%), Postives = 290/383 (75.72%), Query Frame = 0

Query: 2   SVMVENCDGSLLLSLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFA 61
           S+M++NC+G +LLSLDS KSVPAPFLSKTYQLVDDPSTDHIVSWG+D +TFVVWRPPEFA
Sbjct: 2   SLMLDNCEG-ILLSLDSHKSVPAPFLSKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 61

Query: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQ 121
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA PQ
Sbjct: 62  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 121

Query: 122 VSYSTG--PTTFFP---RLSISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIG----- 181
           + Y+     ++FFP   R+SISPSDSD+ + NW CDSPP P       ++++G       
Sbjct: 122 LPYNHHHLGSSFFPFSTRVSISPSDSDEHI-NW-CDSPPLPPRAAPAGFSVLGSANVSNN 181

Query: 182 -------IGSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKPVSPPPTTN 241
                  +GSV ALS+DN+RLRRNN ML+SELAHMRKLYNDIIYFVQNHVKPVSP  +  
Sbjct: 182 SNTSYSMMGSVTALSEDNERLRRNNSMLISELAHMRKLYNDIIYFVQNHVKPVSPSNSFP 241

Query: 242 SFLLSHPA---------PTHNSSQKPLNHFLGFCPNKHTSPGT----------AATAHFL 301
           S LLSHP+         P   +SQKPLN  LGF P  H +  T            T HF+
Sbjct: 242 SLLLSHPSTTTTTPPPPPPPPNSQKPLNQLLGFYPPTHANTTTKHFSFPTANAIPTTHFV 301

Query: 302 DSPTATSKSAITELDAF--DNNSNTCRTKLFGVPLPLSKKRLHPDTT-YGSISS--SNKS 344
            SPTATSKSA+TELD F  + ++++C+TKLFGVPL  SKKR HPDTT Y S SS  S+ +
Sbjct: 302 SSPTATSKSAVTELDTFGMEGDNSSCKTKLFGVPLSSSKKRQHPDTTNYVSTSSHVSDTN 361

BLAST of MS018877 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 312.0 bits (798), Expect = 6.0e-85
Identity = 191/369 (51.76%), Postives = 233/369 (63.14%), Query Frame = 0

Query: 2   SVMVENCDGS--------LLLSLDSQ-KSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTF 61
           ++MVEN  G         + L ++ Q K+VPAPFL+KTYQLVDDP+TDH+VSWG D +TF
Sbjct: 2   AMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTF 61

Query: 62  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEI 121
           VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCEI
Sbjct: 62  VVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEI 121

Query: 122 HRRKT----------------AHPQVSYSTGPTTFFPRLSISPSDSDDQLPNWLCDSPPP 181
           HRRKT                A PQ+ +S G  +FFP     P  +  +  ++ CD  PP
Sbjct: 122 HRRKTSQMIPQQHSPFMSHHHAPPQIPFSGG--SFFP--LPPPRVTTPEEDHYWCDDSPP 181

Query: 182 PLPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQNHVKP 241
             P               V ALS+DN+RLRR+N +L+SELAHM+KLYNDIIYFVQNHVKP
Sbjct: 182 SRPRVIPQQIDTA---AQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKP 241

Query: 242 VSPPPTTNSFLLSHPAPTHNSSQKPLNHFLGFCPNKHTSPGTAATAHFLDSPTATSKSAI 301
           V+  P+ NS  LS         Q P   +       +T+   A   + L+S   TS+S+I
Sbjct: 242 VA--PSNNSSYLSSFLQKQQQQQPPTLDYY------NTATVNATNLNALNSSPPTSQSSI 301

Query: 302 TELDAFDNN----SNTCRTKLFGVPLPLSKKRLHPDTTYGSISSSNKSSSSRLVLEKGDN 342
           T L+    N    SN  +TKLFGV LP SKKR H          S+++S +RLVL++ D 
Sbjct: 302 TVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSH--------HFSDQTSKTRLVLDQSD- 346

BLAST of MS018877 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 182.6 bits (462), Expect = 5.5e-46
Identity = 95/216 (43.98%), Postives = 132/216 (61.11%), Query Frame = 0

Query: 17  DSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFARDLLPNYFKHNNFSS 76
           DSQ+S+P PFL+KTYQLV+DP  D ++SW +D +TF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 77  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQVSYSTGPTTFFPRLS 136
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK + P ++ +          S
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 137 -------------ISPSDSDDQLPNWLCDSPPPPLPTTTTNYNIIGIG-------IGSVA 196
                        +SPS+S ++    +  S   P         ++G G         +  
Sbjct: 171 AVTVAAVPVVAHIVSPSNSGEE---QVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAP 230

Query: 197 ALSQDNDRLRRNNCMLLSELAHMRKLYNDIIYFVQN 213
            L ++N+RLR++N  L  E+  ++ LY +I   + N
Sbjct: 231 ELVEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of MS018877 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 179.9 bits (455), Expect = 3.5e-45
Identity = 97/199 (48.74%), Postives = 129/199 (64.82%), Query Frame = 0

Query: 18  SQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFARDLLPNYFKHNNFSSF 77
           +Q+SVPAPFLSKTYQLVDD STD +VSW ++ + FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 78  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQVSYSTGPTTFFPRLSI 137
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK+    ++ + G        S 
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSV---IASTAGKCVVVGSPSE 126

Query: 138 SPSDSDDQLPNWLCDSP-PPPLPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSEL 197
           S S   D   +    SP     P +  N          VA LS +N++L+R N  L SEL
Sbjct: 127 SNSGGGDDHGSSSTSSPGSSKNPGSVEN---------MVADLSGENEKLKRENNNLSSEL 186

Query: 198 AHMRKLYNDIIYFVQNHVK 216
           A  +K  ++++ F+  H+K
Sbjct: 187 AAAKKQRDELVTFLTGHLK 193

BLAST of MS018877 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-44
Identity = 99/224 (44.20%), Postives = 137/224 (61.16%), Query Frame = 0

Query: 18  SQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFARDLLPNYFKHNNFSSF 77
           SQ+S+P PFL+KT+ LV+D S D ++SW +D S+F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 78  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRK-TAHPQVSYSTGPTTFFPRLS 137
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK T   Q   +         + 
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMV 135

Query: 138 ISPSDS-DDQLPNWLCDSPPPPLPTTTTNYNIIGIGIGSVAALSQDNDRLRRNNCMLLSE 197
           +SPS+S +D   N +  S P       T     G   G    L ++N++LR  N  L  E
Sbjct: 136 VSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNG---GLSVELLEENEKLRSQNIQLNRE 195

Query: 198 LAHMRKLYNDIIYFVQNHVKPVSPPPTTNSFLLSHPAPTHNSSQ 240
           L  M+ + ++I   + N+   V   PT  S+     +P  +SSQ
Sbjct: 196 LTQMKSICDNIYSLMSNY---VGSQPTDRSY-----SPGGSSSQ 228

BLAST of MS018877 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 162.9 bits (411), Expect = 4.5e-40
Identity = 74/114 (64.91%), Postives = 90/114 (78.95%), Query Frame = 0

Query: 15  SLDSQKSVPAPFLSKTYQLVDDPSTDHIVSWGQDLSTFVVWRPPEFARDLLPNYFKHNNF 74
           +L +  S+P PFLSKTY +V+DP+TD IVSW    ++F+VW PPEF+RDLLP YFKHNNF
Sbjct: 42  TLLNANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNF 101

Query: 75  SSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAHPQVSYSTGP 129
           SSFVRQLNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+     S S+ P
Sbjct: 102 SSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNP 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144161.11.4e-19799.13heat stress transcription factor B-4 [Momordica charantia][more]
XP_038885667.18.8e-13167.55heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_004143013.18.8e-13168.78heat stress transcription factor B-4 [Cucumis sativus] >KGN62495.1 hypothetical ... [more]
KAA0060911.13.3e-13068.52heat stress transcription factor B-4 [Cucumis melo var. makuwa][more]
XP_008444610.13.3e-13068.52PREDICTED: heat stress transcription factor B-4 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9C6358.4e-8451.76Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ02.0e-6948.64Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX86.5e-6856.97Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U948.4e-6846.40Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R82.2e-6046.73Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A6J1CSH76.9e-19899.13heat stress transcription factor B-4 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A0A0LKU24.3e-13168.78HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356690 ... [more]
A0A5A7V5511.6e-13068.52Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BAP21.6e-13068.52heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103487881 PE=... [more]
A0A6J1KEA49.2e-12665.80heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G46264.16.0e-8551.76heat shock transcription factor B4 [more]
AT4G11660.15.5e-4643.98winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.13.5e-4548.74heat shock factor 4 [more]
AT5G62020.11.8e-4444.20heat shock transcription factor B2A [more]
AT4G17750.14.5e-4064.91heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 64..76
score: 78.68
coord: 26..49
score: 46.55
coord: 77..89
score: 84.62
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 22..115
e-value: 6.5E-58
score: 208.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 26..115
e-value: 1.5E-31
score: 108.8
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 65..89
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 18..117
e-value: 9.4E-37
score: 127.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..327
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 13..341
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 13..341
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 23..115

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018877.1MS018877.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding