MS018759 (gene) Bitter gourd (TR) v1

Overview
NameMS018759
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtoporphyrinogen oxidase
Locationscaffold313: 1735767 .. 1741279 (+)
RNA-Seq ExpressionMS018759
SyntenyMS018759
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCGGCGCCACCCTCCTCATCGACCTCCCTCTCCGGCGGACTCACCGTCCCTCTCTTCTCCGCCCCTCCAACATTCCCTCCTTCCACCCACTCCACATCTCTCTCCGAAGCAATCACGTTCGCTCCAATTTCAGATGCTCCATCGCCGAGGGCTCCACTCTTTCTGCTTCCAAGGGCACTGGCCCCTCTTCCATGGTGGATTGTGTGGTCGTCGGTGCTGGAATTAGCGGCCTCTGCATCGCTCAGGCCCTCGCCACCAAGCACCCTGATGTCGCCCCCAACATCCTCGTCACGGAGGCGAGAGGTCGCGTTGGAGGCAACATCACCACCGTTGAGAGGGATGGCTATCTCTGGGAAGAAGGTCCTAATAGCTTCCAGCCCTCCGATCCTATACTCACTATGGTGGTTGGTATCATTCTCGTTTTGTCCTATCTTCTCCATTTTTAGGTAGTGTGCTGAATCTCCATTGTTAACAATTACACTAAGGAACCTAATCATTCATGCCGTTCTTCTGAAAATAGACAAGTAAATGATCCTTCTAAGCACTCTGGAAAATATCAATCTAATCTTAAATATGGAGGAAGGTGCCTACTCTAAGTTGCGTTGTTTTGCTGATTGTAGTTACATAGGATTTTATCCTCTAACTAGAATTAGTATAGTGGGACGAGGACGACCTTATCTGAACAGTATTTGTTCTATATCCCTTAACTATTGTGGTGGGATGATCTCCATATTCTTTATTATCTATTGCTGAGGGATTTCTATCCTCGAGATTTTATATATTATCAGTTGTAGGCATGTTTTATATGTATTGTCAGGAAGTGATTTTCTTTCGGTATGTTTTTTGAGTGTCGTTGGTTTTATTTATTGATTCTCATACAATGGCAGGTGGATAGTGGCTTGAAAGATGATTTAGTTCTGGGAGATCCGGATGCACCTCGATTTGTACTGTGGAATGGAAAGCTAAGGCCAGTGCCTGGGAAGCCTACTGATCTACCTTTCTTTGACCTGATGAGTATTGGTGGAAAAATTAGAGCAGGCTTTGGTGCACTGGGCATTCGCCCTCCTCCTCCAGTTTGTCTTCTCAATCCTATGTCTATATATTGGAGTACATTGTCACATCAATCTCAGAAAAAGGGAAATTTTTTGTTTTCAGTTTCGTATATATTGTAATACTGACGAAGGTAACAGGGTCTTGTCACAGGGTCGTGAGGAATCAGTTGAAGAATTTGTTCGTCGGAACCTTGGTGATGAAGTTTTTGAACGCTTGATAGAGCCATTTTGTTCCGGTGATAATCATATTGCATATTTTCTTTATCACACTATGTTTCCTATTATCTTGAGAAGGTTTCTAAATCCTTGTTTCTGGTTAGGATTATATAGTATTGCGGTTATCAAATTAATATTTTCTAGTATTGTGGTAATGTGGTTATCAAATTAATATTTCTTATTCTTATGATAAGCACCATATAGAAGTGATCCAAAGAGTGTAATGAAGTTCTTTCTAACGGTTATTACTTGTTCCTTCTTGACTGTTGAACTCTATTTTCTTTTTGGGACGTTTTTGGCTCACAAGTTAGGTTCACGTAACACAGAGTTAAGAAATAAATCTTATGTTCGATACGTAGGTTTGCTCATGGATAATTCTATGGACTATATTATCCAAACTTATGAAGTCTTCCTTTTATACAACATATTAGCTTTTGTGAACCATAAACCACAAACCAAACATAGGTTGCTCAAACCCAGGTTCTTATAACCTATTCCTTTGCAACATCTAAACACAGATTATAAAAACTTGGACTTCCCAACCCCAAATTTCATAAATTTAGGCTTATTAAAATGGGGATGTTTAAAAAATTCCCTTAGGATGTTGGACGAAGAAAGAAAAGGTGATTGAAGATGTGGATGTCCATTATATAACTTGAGAAAGAACCATATTTGTTACTAGGAACGCTTGAGAGAACAAATTGAATAAGGGTTAATCAACCTTCTTCAACAAATAAGAAAATTTCCATTTTTCCAACCTTCTTTCAGTACTATAGGGTTTCTTAAGCTATGCTTGAGAGGATTATCTCTAAAGAACATGCCAAGGTAGCTAAACAGAAACTTCCCAGCAGGACCTATGTCAATGAAAAAAATGACAAAGGATAAAAGGAACAACCTTTTCTATTGAGGAGGAAAGCTTTCATAAATACTCCTTTTACTCAGGAAAATTGTAATAAGTTTGTATTTTTGAAGTTTGACCTTTTTGGTGGGTTGTCTTGCTCATCGAGTCAATTATTAACCCGAAACTGTCTTGTTCAGGCGTATATGCTGGTGACCCTTCAAAGCTAAGTATGAAAGCAGCTTTTGGAAAGGTTTGGAAGTTGGAGCAAAATGGTGGTAGCATCATTGGTGGGACTTTCAAAGCATTACAAGAGAGGAATAATGCTACCAAGCCACCAAGAGATCCGTAATGGTTTAACTCCACGGAGTTTCTCTGTTTTTCTTTTACTTTTCTCCCCTTTTCATGATATTAAACTTGTTTAATAACCACTTCTTACATTCTAGGCGTCTGCCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTAGGAAAGGACTTGCCATGTTGCCGACTGCTATTTCTACTTGGTAAATTTTGTTGAAAAACAAATTTTCTGACAAGTGTTTTGACTATCAGTCTCTGGATTTATCATCTCTTCAACTTTTCCTATTGGTTGTAGTTTGGGCAGTAAAGTAAAATTGTCTTGGAAGCTATCCAGTGTGAGTAAAGTGGATGATGGAGGTTATTGTTTGACATATGAAACACCAGAAGGACCGGTCTCTATTCTCAGCAGAAGTGTCATCATGACGGTTCCTTCTTATGTTGCCAGCAGTCTGTTGCGTCCAATCTCTGTAATTACTTTCTGGGACTTCAATATGGTTTAGTATATAGTTTTTTGTCGAGGCCTTTCCAGAAGATTTTTTAATGATATTTTCCATATATAATTCCCGCTTTTCCATAGCTTTCTTTAGTGTTAGTAATAGCCGTTGTTTACACTTTTTGGAACCCAGATCACCCGTTAGTCAAAAAATGAAATGGACTCCGACAAATTAATAACTTATATTCCATTTTGTGATGTTTCATCCACACTGGGGTTGAGGATATAATAAGCATGGAATTAACATTGTGGAAACACACTTAAACTTGAGTCAGGTAGCAAAGGATAGGAGGGTAGGATGGTAGCCCACAGCCTGTTTGCAAAGGATCGTCTGAGAAAAACATAGCCTAAATCTTCAGCTTTTTCTTTCACAAGGAGCTTTTTCTTTGCTGATTCCATTGTGATTATTCATTAAACAGGTAGCAAAGGAGCTTTTTCTCCTATTATCTTTGCATGTATTTGTGTGAGTAAAAGGCTAGTAGATTGAAGAAGCTGTGCCATGCTGAATTGATGCTGCTTATTTACCCAATTCCTTGGAATTAGTTTGCCTGTGAATTGCATAAATGTTGGCCTGATTTGAGTAAAAATTGCAAAGGCTACTAAGTACATCTTCTGTGGTTTCTATTTGCAGTATATACACCTATCTAGTGATTTCCCAAGTTGGAATAAAGTATATGCATCCAACTTCTAAGCAACTTAATTTACTCACTAGTGCAGAGTACATTTATCTCTCAAAACCTGTCAAGTTGTGCACCTGGTGCAAGAGTAGGATGTCCAGCATTTGTTCTGTTTTCAGGTTTGTTTCTTTTTGCTGATTCCCTTGCCTGCAGGTGACAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACTATATCATATCCAAAAGGAGCAATCAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTCGGTCAACTGCACCCTCGCAGCCAGGGGGTGACAACTTTGGGTAAATCTGCTTTCTATACACTTTATGTGGCATTCTGCCCCAAACTTATGTTAAGATAACATTGCCTTTAAGTCTTACAGCTGTAACTGTTAGGTAATTAAGATGGATCCAAATCCATGCTTGGTTTGTTGCAGGAACTATATACAGCTCCTCACTTTTTCCCAATCGAGCACCAGATGGAAGGGTATTACTCTTGAACTACATTGGAGGGGCTACAAATACTGAAATTCTTTCCCAGGTAAAATTTCTTCTACCACAGTTTGGAAACGATCATTCTGAACCATATTTAAAGTTTTGTTGGGAAAACTAAATGCAAAAGGCTTAGTGATGGAATGATGATTAATACCTGTGAGGAAAGTGTCAAGTATCTCTTGTAACTCACTCTTTTAGATAAGAGACTTAAATAATTAACAACCATTCTGCTTACAGGTAACCAAACAGTTCAGATATTCCAATGAAAACACCCAAACCACTAAACTTAACTCCATCTGGATTTATTACTGATTGTTGGTTGCTCATATATGGTTGAATACCACTTAAATTCCATCTTCTAAGCCTACCTTGTTTCCTTGTAGCATTATTTTGCTCATCATAAGACTCAGATTCTTGTTACTATTGTTCTTATGTGATAACAGACAGAGAGTGAGCTTGTTGACGCAGTTGATCGGGATTTGAGAAAAATCCTCATAAACCCAAACGCAGAGGACCCCCTGCTATTGAGTGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATAGGTCATCTTGATGTTTTGGATGCGGCCAAGGCTGGGCTGAGAGAAGCTGGAATGGAGGGGCTGTTTCTGGGCGGAAACTATGTGTGTGGTGTGGCCTTGGGGCGATGTGTGGAGGGTGCCTATGAAGTTGCAGCTCAGGTAGCTGGTTTCCTGTCCCAAAAAGTGTATAAATAGCTGCTCTCTAACTAATAGGCTATATTCATGACGTAGAGATGGAAGCAATGGCGATGTTGTAGGGTATTAGCTTGATTTTGTCGCATGCTTAATTGCCAGTAAATCAGTAACAAATAGTCGTAATCCTATTTTTGGTATGATTGCCCACAGTATTAGTGTGCTAAACAGAGAACAAATACACATTTCACCGTTTCAAATGAAATCAGAAAACAGAAAGTGAATATTGGTGGGTTTAAACTCAGGATACACATCTACTTTAAGAATTAACTTTTACGTCACCAAATGTGGTAAGGTTAGGCAGCTGTTAGTGAGATTAGTCAAGGTGCGTGTAAGGTGGTTCGGACACTCGTTGTTATATATATATAAAAAGAAAAAGTGAATATGAGTGAGGATTCTTTTATTCATGCAAATTCAATCATCATAGGGAAAACTAAAAAGACTTCTGATTCTGGCAATCCAAATTGGGGCCAGTATCGACATTGAAGAATTGAGCATATCTTTTGAAGAAGCCGATACGGTCATTGACTCGGGCATCGTCGGGAATCCCACACTCGAGTCCGCCATTGATGATGTTGGTGACGAGGCCGTAGCCTGCAGTTCGGTTGGCTTGTATGTCGGCCTTTGTGGGAACGTATCGACCGACCATGACATCGTGGCA

mRNA sequence

ATGGCTACCGGCGCCACCCTCCTCATCGACCTCCCTCTCCGGCGGACTCACCGTCCCTCTCTTCTCCGCCCCTCCAACATTCCCTCCTTCCACCCACTCCACATCTCTCTCCGAAGCAATCACGTTCGCTCCAATTTCAGATGCTCCATCGCCGAGGGCTCCACTCTTTCTGCTTCCAAGGGCACTGGCCCCTCTTCCATGGTGGATTGTGTGGTCGTCGGTGCTGGAATTAGCGGCCTCTGCATCGCTCAGGCCCTCGCCACCAAGCACCCTGATGTCGCCCCCAACATCCTCGTCACGGAGGCGAGAGGTCGCGTTGGAGGCAACATCACCACCGTTGAGAGGGATGGCTATCTCTGGGAAGAAGGTCCTAATAGCTTCCAGCCCTCCGATCCTATACTCACTATGGTGGTGGATAGTGGCTTGAAAGATGATTTAGTTCTGGGAGATCCGGATGCACCTCGATTTGTACTGTGGAATGGAAAGCTAAGGCCAGTGCCTGGGAAGCCTACTGATCTACCTTTCTTTGACCTGATGAGTATTGGTGGAAAAATTAGAGCAGGCTTTGGTGCACTGGGCATTCGCCCTCCTCCTCCAGGTCGTGAGGAATCAGTTGAAGAATTTGTTCGTCGGAACCTTGGTGATGAAGTTTTTGAACGCTTGATAGAGCCATTTTGTTCCGGCGTATATGCTGGTGACCCTTCAAAGCTAAGTATGAAAGCAGCTTTTGGAAAGGTTTGGAAGTTGGAGCAAAATGGTGGTAGCATCATTGGTGGGACTTTCAAAGCATTACAAGAGAGGAATAATGCTACCAAGCCACCAAGAGATCCGCGTCTGCCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTAGGAAAGGACTTGCCATGTTGCCGACTGCTATTTCTACTTGTTTGGGCAGTAAAGTAAAATTGTCTTGGAAGCTATCCAGTGTGAGTAAAGTGGATGATGGAGGTTATTGTTTGACATATGAAACACCAGAAGGACCGGTCTCTATTCTCAGCAGAAGTGTCATCATGACGGTTCCTTCTTATGTTGCCAGCAGTCTGTTGCGTCCAATCTCTGTGACAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACTATATCATATCCAAAAGGAGCAATCAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTCGGTCAACTGCACCCTCGCAGCCAGGGGGTGACAACTTTGGGAACTATATACAGCTCCTCACTTTTTCCCAATCGAGCACCAGATGGAAGGGTATTACTCTTGAACTACATTGGAGGGGCTACAAATACTGAAATTCTTTCCCAGACAGAGAGTGAGCTTGTTGACGCAGTTGATCGGGATTTGAGAAAAATCCTCATAAACCCAAACGCAGAGGACCCCCTGCTATTGAGTGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATAGGTCATCTTGATGTTTTGGATGCGGCCAAGGCTGGGCTGAGAGAAGCTGGAATGGAGGGGCTGTTTCTGGGCGGAAACTATGTGTGTGGTGTGGCCTTGGGGCGATGTGTGGAGGGTGCCTATGAAGTTGCAGCTCAGGTAGCTGGTTTCCTGTCCCAAAAAGGAAAACTAAAAAGACTTCTGATTCTGGCAATCCAAATTGGGGCCAGTATCGACATTGAAGAATTGAGCATATCTTTTGAAGAAGCCGATACGGTCATTGACTCGGGCATCGTCGGGAATCCCACACTCGAGTCCGCCATTGATGATGTTGGTGACGAGGCCGTAGCCTGCAGTTCGGTTGGCTTGTATGTCGGCCTTTGTGGGAACGTATCGACCGACCATGACATCGTGGCA

Coding sequence (CDS)

ATGGCTACCGGCGCCACCCTCCTCATCGACCTCCCTCTCCGGCGGACTCACCGTCCCTCTCTTCTCCGCCCCTCCAACATTCCCTCCTTCCACCCACTCCACATCTCTCTCCGAAGCAATCACGTTCGCTCCAATTTCAGATGCTCCATCGCCGAGGGCTCCACTCTTTCTGCTTCCAAGGGCACTGGCCCCTCTTCCATGGTGGATTGTGTGGTCGTCGGTGCTGGAATTAGCGGCCTCTGCATCGCTCAGGCCCTCGCCACCAAGCACCCTGATGTCGCCCCCAACATCCTCGTCACGGAGGCGAGAGGTCGCGTTGGAGGCAACATCACCACCGTTGAGAGGGATGGCTATCTCTGGGAAGAAGGTCCTAATAGCTTCCAGCCCTCCGATCCTATACTCACTATGGTGGTGGATAGTGGCTTGAAAGATGATTTAGTTCTGGGAGATCCGGATGCACCTCGATTTGTACTGTGGAATGGAAAGCTAAGGCCAGTGCCTGGGAAGCCTACTGATCTACCTTTCTTTGACCTGATGAGTATTGGTGGAAAAATTAGAGCAGGCTTTGGTGCACTGGGCATTCGCCCTCCTCCTCCAGGTCGTGAGGAATCAGTTGAAGAATTTGTTCGTCGGAACCTTGGTGATGAAGTTTTTGAACGCTTGATAGAGCCATTTTGTTCCGGCGTATATGCTGGTGACCCTTCAAAGCTAAGTATGAAAGCAGCTTTTGGAAAGGTTTGGAAGTTGGAGCAAAATGGTGGTAGCATCATTGGTGGGACTTTCAAAGCATTACAAGAGAGGAATAATGCTACCAAGCCACCAAGAGATCCGCGTCTGCCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTAGGAAAGGACTTGCCATGTTGCCGACTGCTATTTCTACTTGTTTGGGCAGTAAAGTAAAATTGTCTTGGAAGCTATCCAGTGTGAGTAAAGTGGATGATGGAGGTTATTGTTTGACATATGAAACACCAGAAGGACCGGTCTCTATTCTCAGCAGAAGTGTCATCATGACGGTTCCTTCTTATGTTGCCAGCAGTCTGTTGCGTCCAATCTCTGTGACAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACTATATCATATCCAAAAGGAGCAATCAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTCGGTCAACTGCACCCTCGCAGCCAGGGGGTGACAACTTTGGGAACTATATACAGCTCCTCACTTTTTCCCAATCGAGCACCAGATGGAAGGGTATTACTCTTGAACTACATTGGAGGGGCTACAAATACTGAAATTCTTTCCCAGACAGAGAGTGAGCTTGTTGACGCAGTTGATCGGGATTTGAGAAAAATCCTCATAAACCCAAACGCAGAGGACCCCCTGCTATTGAGTGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATAGGTCATCTTGATGTTTTGGATGCGGCCAAGGCTGGGCTGAGAGAAGCTGGAATGGAGGGGCTGTTTCTGGGCGGAAACTATGTGTGTGGTGTGGCCTTGGGGCGATGTGTGGAGGGTGCCTATGAAGTTGCAGCTCAGGTAGCTGGTTTCCTGTCCCAAAAAGGAAAACTAAAAAGACTTCTGATTCTGGCAATCCAAATTGGGGCCAGTATCGACATTGAAGAATTGAGCATATCTTTTGAAGAAGCCGATACGGTCATTGACTCGGGCATCGTCGGGAATCCCACACTCGAGTCCGCCATTGATGATGTTGGTGACGAGGCCGTAGCCTGCAGTTCGGTTGGCTTGTATGTCGGCCTTTGTGGGAACGTATCGACCGACCATGACATCGTGGCA

Protein sequence

MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASKGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQKGKLKRLLILAIQIGASIDIEELSISFEEADTVIDSGIVGNPTLESAIDDVGDEAVACSSVGLYVGLCGNVSTDHDIVA
Homology
BLAST of MS018759 vs. NCBI nr
Match: XP_022146407.1 (protoporphyrinogen oxidase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASK 60
           MATGATLLIDLPLRRTHRPSLLR SNIPSFHPLHISLR NHVRSNFRCSIAEGSTLSASK
Sbjct: 1   MATGATLLIDLPLRRTHRPSLLRHSNIPSFHPLHISLRCNHVRSNFRCSIAEGSTLSASK 60

Query: 61  GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW 120
           GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW
Sbjct: 61  GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW 120

Query: 121 EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 180
           EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS
Sbjct: 121 EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 180

Query: 181 IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 240
           IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 181 IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 240

Query: 241 AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA 300
           AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA
Sbjct: 241 AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA 300

Query: 301 ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI 360
           ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI
Sbjct: 301 ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI 360

Query: 361 SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 420
           SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS
Sbjct: 361 SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 420

Query: 421 SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVR 480
           SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELV+AVDRDLRKILINPNAEDPLLLSVR
Sbjct: 421 SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVEAVDRDLRKILINPNAEDPLLLSVR 480

Query: 481 VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG 540
           VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG
Sbjct: 481 VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG 540

Query: 541 FLSQK 546
           FLS+K
Sbjct: 541 FLSRK 545

BLAST of MS018759 vs. NCBI nr
Match: XP_038875429.1 (protoporphyrinogen oxidase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.3e-289
Identity = 500/546 (91.58%), Postives = 519/546 (95.05%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATGATLL DLP RR H  +LLRPSNIPSFHPLH+SLR+NH+R NFRCSIAEGST LS S
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSNIPSFHPLHVSLRNNHLRPNFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
           K +G SSMVDCVVVG GISGLCIAQAL TKHP+VAPNI+VTEA+ RVGGNITTVERDGYL
Sbjct: 61  KASGQSSMVDCVVVGGGISGLCIAQALQTKHPNVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLEQNGGSIIGGTFKA+QERN ATKPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAIQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVKLSWKLSS+SKVDDGGY LTYETPEG VSILSRSVIMTVPSY+A +LLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS  AADALSKFYYPPVASVTISYPKGAIRKECL DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLSDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILS+TESELV+ VD+DLRKILINPNAEDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDQDLRKILINPNAEDPLSLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLD+LD AKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA+VA
Sbjct: 481 RVWPQAIPQFLIGHLDLLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYETAAEVA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. NCBI nr
Match: XP_023547363.1 (protoporphyrinogen oxidase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 998.0 bits (2579), Expect = 3.5e-287
Identity = 497/546 (91.03%), Postives = 518/546 (94.87%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATGATLL D P RR H  +LLR SNIPSFHPL ISLR N +R NFRCSIAEGST LS S
Sbjct: 1   MATGATLLTDHPFRRPHLSTLLRSSNIPSFHPLRISLRINRLRPNFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
           KG+ PSS+VDCVV+G GISGLCIAQALATKH DVAPNILVTEARGRVGGNITTVERDGYL
Sbjct: 61  KGSAPSSIVDCVVIGGGISGLCIAQALATKHSDVAPNILVTEARGRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLW+GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWDGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLEQNGGSIIGGTFKA QERN A KPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKADKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVKLSWKLSS+SK+DDGGY LTYETPEGP+SILSRSVIMTVPSYVAS+LLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKMDDGGYSLTYETPEGPISILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISVTAADALSKFYYPPVASVTISYPK AIRKE L+DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVTAADALSKFYYPPVASVTISYPKEAIRKERLVDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGG+TNTEIL++TESELV+AVDRDLRKI+IN NAEDPLLLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGSTNTEILAKTESELVEAVDRDLRKIIINTNAEDPLLLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLD+LD AKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA++A
Sbjct: 481 RVWPQAIPQFLIGHLDLLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEIA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. NCBI nr
Match: XP_022990612.1 (protoporphyrinogen oxidase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 995.3 bits (2572), Expect = 2.3e-286
Identity = 496/546 (90.84%), Postives = 517/546 (94.69%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATGATLL D P RR H  +LLR SNI SFHPL ISLR N +R NFRCSIAEGST LS S
Sbjct: 1   MATGATLLTDHPFRRPHLSTLLRSSNISSFHPLRISLRINRLRPNFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
           KG+ PSS+VDCVV+G GISGLCIAQALATKH DVAPNILVTEARGRVGGNITTVERDGYL
Sbjct: 61  KGSAPSSIVDCVVIGGGISGLCIAQALATKHSDVAPNILVTEARGRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLW+GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWDGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLEQNGGSIIGGTFKA QERN A KPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKANKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVKLSWKLSS+SK+DDGGY LTYETPEGP+SILSRSVIMTVPSYVAS+LLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKMDDGGYSLTYETPEGPISILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISVT ADALSKFYYPPVASVTISYPK AIRKE L+DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVTTADALSKFYYPPVASVTISYPKEAIRKERLVDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGG+TNTEIL++TESELV+AVDRDLRKI+IN NAEDPLLLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGSTNTEILAKTESELVEAVDRDLRKIIINTNAEDPLLLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLD+LDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA++A
Sbjct: 481 RVWPQAIPQFLIGHLDLLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEIA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. NCBI nr
Match: XP_004149479.1 (protoporphyrinogen oxidase 1, chloroplastic [Cucumis sativus] >KGN49787.1 hypothetical protein Csa_004680 [Cucumis sativus])

HSP 1 Score: 995.3 bits (2572), Expect = 2.3e-286
Identity = 496/546 (90.84%), Postives = 516/546 (94.51%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATG+TLL DLP RR H  +LLRPSNIPSFHPLHISL++N +RS FRCSIAEGST LS S
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
             +  SS++DCVVVGAGISGLCIAQALATKHPDVAPNI+VTEA+ RVGGNITTVERDGYL
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVW+LEQNGGSIIGGTFKALQERN  TKPPRDPRLPKPKGQTVGSFRKGL MLP 
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVK+SWKLSS+SKVDDGGY LTYETPEG VSILSRSVIMTVPSY+A +LLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS  AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILSQTESEL++ VDRDLRKILINPNAEDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLDVLD AKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA+VA
Sbjct: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. ExPASy Swiss-Prot
Match: P55826 (Protoporphyrinogen oxidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPOX1 PE=1 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.3e-236
Identity = 411/531 (77.40%), Postives = 458/531 (86.25%), Query Frame = 0

Query: 20  SLLRP---SNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASK---GTGPSSMVDCVVV 79
           SLLRP   S +PSF   ++ L + +     RCS+A G T+ +SK   G G +   DCV+V
Sbjct: 4   SLLRPTTQSLLPSFSKPNLRL-NVYKPLRLRCSVAGGPTVGSSKIEGGGGTTITTDCVIV 63

Query: 80  GAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSDPI 139
           G GISGLCIAQALATKHPD APN++VTEA+ RVGGNI T E +G+LWEEGPNSFQPSDP+
Sbjct: 64  GGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQPSDPM 123

Query: 140 LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALG 199
           LTMVVDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMSIGGKIRAGFGALG
Sbjct: 124 LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAGFGALG 183

Query: 200 IRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 259
           IRP PPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG
Sbjct: 184 IRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 243

Query: 260 GSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSW 319
           GSIIGGTFKA+QER NA K  RDPRLPKP+GQTVGSFRKGL MLP AIS  LGSKVKLSW
Sbjct: 244 GSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSKVKLSW 303

Query: 320 KLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYY 379
           KLS ++K++ GGY LTYETP+G VS+ S+SV+MTVPS+VAS LLRP+S +AA+ALSK YY
Sbjct: 304 KLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANALSKLYY 363

Query: 380 PPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVL 439
           PPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP GR+L
Sbjct: 364 PPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAPPGRIL 423

Query: 440 LLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGH 499
           LLNYIGG+TNT ILS++E ELV+AVDRDLRK+LI PN+ DPL L VRVWPQAIPQFL+GH
Sbjct: 424 LLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVRVWPQAIPQFLVGH 483

Query: 500 LDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQ 545
            D+LD AK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A +V  F+S+
Sbjct: 484 FDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFMSR 533

BLAST of MS018759 vs. ExPASy Swiss-Prot
Match: O24163 (Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI PE=2 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 391/523 (74.76%), Postives = 446/523 (85.28%), Query Frame = 0

Query: 26  NIPSFHPLHISLRSNHVRSN---FRCSIAEGSTL-SASKGTGPSSMVDCVVVGAGISGLC 85
           N  SF P     + N V  N    RCS+A+  T+ S++   GP++ +DCV+VGAGISGLC
Sbjct: 26  NRTSFIPFSSISKRNSVNCNGWRTRCSVAKDYTVPSSAVDGGPAAELDCVIVGAGISGLC 85

Query: 86  IAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSDPILTMVVDSG 145
           IAQ ++  +    PN++VTEAR R GGNITTVERDGYLWEEGPNSFQPSDP+LTM VD G
Sbjct: 86  IAQVMSANY----PNLMVTEARDRAGGNITTVERDGYLWEEGPNSFQPSDPMLTMAVDCG 145

Query: 146 LKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALGIRPPPPGR 205
           LKDDLVLGDP+APRFVLW GKLRPVP K TDL FFDLMSI GK+RAGFGA+G+RP PPG 
Sbjct: 146 LKDDLVLGDPNAPRFVLWKGKLRPVPSKLTDLAFFDLMSIPGKLRAGFGAIGLRPSPPGH 205

Query: 206 EESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTF 265
           EESVE+FVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLE+ GGSIIGGTF
Sbjct: 206 EESVEQFVRRNLGGEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEETGGSIIGGTF 265

Query: 266 KALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSWKLSSVSKV 325
           KA++ER++  K PRDPRLPKPKGQTVGSFRKGL MLP AIS  LGSK+KLSWKLSS++K 
Sbjct: 266 KAIKERSSTPKAPRDPRLPKPKGQTVGSFRKGLRMLPDAISARLGSKLKLSWKLSSITKS 325

Query: 326 DDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYYPPVASVTI 385
           + GGY LTYETPEG VS+ SRS++MTVPSYVAS++LRP+SV AADALS FYYPPV +VTI
Sbjct: 326 EKGGYHLTYETPEGVVSLQSRSIVMTVPSYVASNILRPLSVAAADALSNFYYPPVGAVTI 385

Query: 386 SYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGA 445
           +YP+ AIR E L+DGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP GRVLLLNYIGGA
Sbjct: 386 TYPQEAIRDERLVDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAPKGRVLLLNYIGGA 445

Query: 446 TNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGHLDVLDAAK 505
            N EILS+TES+LV+ VDRDLRK+LI P A+DPL++ VRVWPQAIPQFL+GHLD L  AK
Sbjct: 446 KNPEILSKTESQLVEVVDRDLRKMLIKPKAQDPLVVGVRVWPQAIPQFLVGHLDTLSTAK 505

Query: 506 AGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQ 545
           A + + G+EGLFLGGNYV GVALGRCVEGAYEVA++V GFLS+
Sbjct: 506 AAMNDNGLEGLFLGGNYVSGVALGRCVEGAYEVASEVTGFLSR 544

BLAST of MS018759 vs. ExPASy Swiss-Prot
Match: Q9AR38 (Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PPOX1 PE=2 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 3.2e-222
Identity = 387/517 (74.85%), Postives = 435/517 (84.14%), Query Frame = 0

Query: 32  PLHI--SLRSNHVRSNFRCSIAEGSTLSASKGTGPSSMVDCVVVGAGISGLCIAQALATK 91
           PL I  + R    R + RC++A G+   A    G     DCVVVG GISGLC AQALATK
Sbjct: 19  PLRIRDAARRTRRRGHVRCAVASGAA-EAPAAPGARVSADCVVVGGGISGLCTAQALATK 78

Query: 92  HPDVAPNILVTEARGRVGGNITTVER--DGYLWEEGPNSFQPSDPILTMVVDSGLKDDLV 151
           H     ++LVTEAR R GGNITT ER  +GYLWEEGPNSFQPSDP+LTM VDSGLKDDLV
Sbjct: 79  HG--VGDVLVTEARARPGGNITTAERAGEGYLWEEGPNSFQPSDPVLTMAVDSGLKDDLV 138

Query: 152 LGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVEE 211
            GDP+APRFVLW GKLRPVP KP DLPFFDLMSI GK+RAG GALG+R PPPGREESVE+
Sbjct: 139 FGDPNAPRFVLWEGKLRPVPSKPGDLPFFDLMSIPGKLRAGLGALGVRAPPPGREESVED 198

Query: 212 FVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTFKALQER 271
           FVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAFGKVW+LE  GGSIIGGT K +QER
Sbjct: 199 FVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEDTGGSIIGGTIKTIQER 258

Query: 272 NNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSWKLSSVSKVDDGGYC 331
               KPPRDPRLP PKGQTV SFRKGL MLP AI++ LGSKVKLSWKL+S++K D+ GY 
Sbjct: 259 GKNPKPPRDPRLPTPKGQTVASFRKGLTMLPDAITSRLGSKVKLSWKLTSITKSDNKGYA 318

Query: 332 LTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYYPPVASVTISYPKGA 391
           L YETPEG VS+ +++V+MT+PSYVAS +LRP+S  AADALS FYYPPVA+VT+SYPK A
Sbjct: 319 LVYETPEGVVSVQAKTVVMTIPSYVASDILRPLSSDAADALSIFYYPPVAAVTVSYPKEA 378

Query: 392 IRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEIL 451
           IRKECLIDGEL+GFGQLHPRSQGV TLGTIYSSSLFPNRAP GRVLLLNYIGG+TNT I+
Sbjct: 379 IRKECLIDGELQGFGQLHPRSQGVETLGTIYSSSLFPNRAPAGRVLLLNYIGGSTNTGIV 438

Query: 452 SQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGHLDVLDAAKAGLREA 511
           S+TESELV+AVDRDLRK+LINP A DPL+L VRVWPQAIPQFLIGHLD L+AAK+ L + 
Sbjct: 439 SKTESELVEAVDRDLRKMLINPKAVDPLVLGVRVWPQAIPQFLIGHLDHLEAAKSALGKG 498

Query: 512 GMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQ 545
           G +GLFLGGNYV GVALGRCVEGAYE A+Q++ +L++
Sbjct: 499 GYDGLFLGGNYVAGVALGRCVEGAYESASQISDYLTK 532

BLAST of MS018759 vs. ExPASy Swiss-Prot
Match: Q9LRI8 (Protoporphyrinogen oxidase 1, chloroplastic OS=Spinacia oleracea OX=3562 GN=POX1 PE=1 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 4.7e-218
Identity = 388/565 (68.67%), Postives = 449/565 (79.47%), Query Frame = 0

Query: 1   MATGATLLIDLPLR-------RTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEG 60
           MA  +T+ + LP         R +R ++L PS+         SLR     S+ RCS    
Sbjct: 4   MALSSTMALSLPQSSMSLSHCRHNRITILIPSS---------SLR-RRGGSSIRCSTIST 63

Query: 61  STLSASK--------------GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILV 120
           S  +A+               G G   ++DCV+VG GISGLCIAQAL+TK+ +++ N +V
Sbjct: 64  SNSAAAANYQNKNIGTNGVDGGGGGGGVLDCVIVGGGISGLCIAQALSTKYSNLSTNFIV 123

Query: 121 TEARGRVGGNITTVERDGYLWEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLW 180
           TEA+ RVGGNITT+E DGYLWEEGPNSFQPSD +LTM VDSGLK++LVLGDP++PRFVLW
Sbjct: 124 TEAKDRVGGNITTMEADGYLWEEGPNSFQPSDAVLTMAVDSGLKEELVLGDPNSPRFVLW 183

Query: 181 NGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFE 240
           NGKLRPVP K TDLPFFDLMS  GKIRAG GALG+RP PP  EESVE+FVRRNLGDEVFE
Sbjct: 184 NGKLRPVPSKLTDLPFFDLMSFPGKIRAGLGALGLRPSPPAHEESVEQFVRRNLGDEVFE 243

Query: 241 RLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRL 300
           RLIEPFCSGVYAGDPSKLSMKAAFG+VW LEQ GGSIIGGT K +QER +  KPPRDPRL
Sbjct: 244 RLIEPFCSGVYAGDPSKLSMKAAFGRVWVLEQKGGSIIGGTLKTIQERKDNPKPPRDPRL 303

Query: 301 PKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSI 360
           PKPKGQTVGSFRKGL+MLPTAIS  LG+KVK+SW LS ++K  +G Y LTYETP+G VS+
Sbjct: 304 PKPKGQTVGSFRKGLSMLPTAISERLGNKVKVSWTLSGIAKSSNGEYNLTYETPDGLVSV 363

Query: 361 LSRSVIMTVPSYVASSLLRPISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELK 420
            ++SV+MTVPSYVASSLLRP+S  AA++LSKF+YPPVA+V++SYPK AIR ECLIDGELK
Sbjct: 364 RTKSVVMTVPSYVASSLLRPLSDVAAESLSKFHYPPVAAVSLSYPKEAIRSECLIDGELK 423

Query: 421 GFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVD 480
           GFGQLH RSQGV TLGTIYSSSLFP RAP GR L+LNYIGG TN  IL +T+ EL +AVD
Sbjct: 424 GFGQLHSRSQGVETLGTIYSSSLFPGRAPPGRTLILNYIGGDTNPGILDKTKDELAEAVD 483

Query: 481 RDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYV 540
           RDLR+ILINPNA+ P +L VRVWPQAIPQFLIGH D+LDAAKA L + G +GLFLGGNYV
Sbjct: 484 RDLRRILINPNAKAPRVLGVRVWPQAIPQFLIGHFDLLDAAKAALTDGGHKGLFLGGNYV 543

Query: 541 CGVALGRCVEGAYEVAAQVAGFLSQ 545
            GVALGRC+EGAYE AA+V  FLSQ
Sbjct: 544 SGVALGRCIEGAYESAAEVVDFLSQ 558

BLAST of MS018759 vs. ExPASy Swiss-Prot
Match: P56601 (Coproporphyrinogen III oxidase OS=Myxococcus xanthus OX=34 GN=cgoX PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.7e-53
Identity = 154/476 (32.35%), Postives = 237/476 (49.79%), Query Frame = 0

Query: 68  VDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSF 127
           ++  VVG GISGL +A  L ++  D     ++ E+  R+GG + T    GYL E+GPNSF
Sbjct: 10  MNVAVVGGGISGLAVAHHLRSRGTDA----VLLESSARLGGAVGTHALAGYLVEQGPNSF 69

Query: 128 QPSDPIL-TMVVDSGLKDDLVLGDPDAP-RFVLWNGKLRPVPGKPTDLPFFDLMSIGGKI 187
              +P    +     L+  +   DP A  R+V   G+LR VP  P      D++ +G ++
Sbjct: 70  LDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARL 129

Query: 188 RAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGK 247
           R   G L  R  P G +ES+  F RR+LG    + L++   +G+YAGD  +LS+ A F  
Sbjct: 130 RVA-GELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPM 189

Query: 248 VWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCL 307
           + K+E+   S+I G  +A + +  A  P      PK  G  + +F  GL +L  A++  L
Sbjct: 190 LVKMEREHRSLILGAIRAQKAQRQAALPA--GTAPKLSG-ALSTFDGGLQVLIDALAASL 249

Query: 308 GSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAA 367
           G    +  ++  +++ +DGG+ L  E       +    V++  P++  + LLRP+    A
Sbjct: 250 GDAAHVGARVEGLAR-EDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALA 309

Query: 368 DALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPN 427
             ++   Y P+A V + +  G +           GFG L P  +    LG I++S+ FP 
Sbjct: 310 ALVAGIAYAPIAVVHLGFDAGTLPAP-------DGFGFLVPAEEQRRMLGAIHASTTFPF 369

Query: 428 RAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQA 487
           RA  GRVL    +GGA    ++ Q E  L  A+ R+  K L    A  P    V  WP  
Sbjct: 370 RAEGGRVLYSCMVGGARQPGLVEQDEDALA-ALAREELKALAGVTAR-PSFTRVFRWPLG 429

Query: 488 IPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVA-AQVAG 541
           IPQ+ +GHL+ + A  A L+   + GL L GN   GV L  C+  A ++A A VAG
Sbjct: 430 IPQYNLGHLERVAAIDAALQR--LPGLHLIGNAYKGVGLNDCIRNAAQLADALVAG 465

BLAST of MS018759 vs. ExPASy TrEMBL
Match: A0A6J1CZI0 (Protoporphyrinogen oxidase OS=Momordica charantia OX=3673 GN=LOC111015630 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASK 60
           MATGATLLIDLPLRRTHRPSLLR SNIPSFHPLHISLR NHVRSNFRCSIAEGSTLSASK
Sbjct: 1   MATGATLLIDLPLRRTHRPSLLRHSNIPSFHPLHISLRCNHVRSNFRCSIAEGSTLSASK 60

Query: 61  GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW 120
           GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW
Sbjct: 61  GTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLW 120

Query: 121 EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 180
           EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS
Sbjct: 121 EEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMS 180

Query: 181 IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 240
           IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK
Sbjct: 181 IGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMK 240

Query: 241 AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA 300
           AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA
Sbjct: 241 AAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTA 300

Query: 301 ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI 360
           ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI
Sbjct: 301 ISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPI 360

Query: 361 SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 420
           SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS
Sbjct: 361 SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSS 420

Query: 421 SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVR 480
           SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELV+AVDRDLRKILINPNAEDPLLLSVR
Sbjct: 421 SLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVEAVDRDLRKILINPNAEDPLLLSVR 480

Query: 481 VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG 540
           VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG
Sbjct: 481 VWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAG 540

Query: 541 FLSQK 546
           FLS+K
Sbjct: 541 FLSRK 545

BLAST of MS018759 vs. ExPASy TrEMBL
Match: A0A6J1JJ97 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111487440 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.1e-286
Identity = 496/546 (90.84%), Postives = 517/546 (94.69%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATGATLL D P RR H  +LLR SNI SFHPL ISLR N +R NFRCSIAEGST LS S
Sbjct: 1   MATGATLLTDHPFRRPHLSTLLRSSNISSFHPLRISLRINRLRPNFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
           KG+ PSS+VDCVV+G GISGLCIAQALATKH DVAPNILVTEARGRVGGNITTVERDGYL
Sbjct: 61  KGSAPSSIVDCVVIGGGISGLCIAQALATKHSDVAPNILVTEARGRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLW+GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWDGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLEQNGGSIIGGTFKA QERN A KPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKANKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVKLSWKLSS+SK+DDGGY LTYETPEGP+SILSRSVIMTVPSYVAS+LLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKMDDGGYSLTYETPEGPISILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISVT ADALSKFYYPPVASVTISYPK AIRKE L+DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVTTADALSKFYYPPVASVTISYPKEAIRKERLVDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGG+TNTEIL++TESELV+AVDRDLRKI+IN NAEDPLLLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGSTNTEILAKTESELVEAVDRDLRKIIINTNAEDPLLLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLD+LDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA++A
Sbjct: 481 RVWPQAIPQFLIGHLDLLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEIA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. ExPASy TrEMBL
Match: A0A0A0KMZ2 (Protoporphyrinogen oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G128250 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.1e-286
Identity = 496/546 (90.84%), Postives = 516/546 (94.51%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATG+TLL DLP RR H  +LLRPSNIPSFHPLHISL++N +RS FRCSIAEGST LS S
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
             +  SS++DCVVVGAGISGLCIAQALATKHPDVAPNI+VTEA+ RVGGNITTVERDGYL
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVW+LEQNGGSIIGGTFKALQERN  TKPPRDPRLPKPKGQTVGSFRKGL MLP 
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVK+SWKLSS+SKVDDGGY LTYETPEG VSILSRSVIMTVPSY+A +LLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           IS  AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNT ILSQTESEL++ VDRDLRKILINPNAEDPL LSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLDVLD AKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA+VA
Sbjct: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. ExPASy TrEMBL
Match: A0A6J1HHU4 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463722 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 7.2e-286
Identity = 494/546 (90.48%), Postives = 518/546 (94.87%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGST-LSAS 60
           MATGATLL D P RR H  +LLR SNIPSFHPL IS+R N +R NFRCSIAEGST LS S
Sbjct: 1   MATGATLLTDHPFRRPHLSTLLRSSNIPSFHPLRISIRINRLRPNFRCSIAEGSTALSPS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
           KG+ PSS+VDCVV+G GISGLCIAQALATKH DVAPNILVTEARGRVGGNITTVERDGYL
Sbjct: 61  KGSAPSSIVDCVVIGGGISGLCIAQALATKHSDVAPNILVTEARGRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLW+GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWDGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLE+NGGSIIGGTFKA QERN A KPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEKNGGSIIGGTFKAFQERNKANKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLGSKVKLSWKLSS+SK+DDGGY LTYETPEG +SILS+SVIMTVPSYVAS+LLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKMDDGGYSLTYETPEGLISILSKSVIMTVPSYVASTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISVTAADALSKFYYPPVASVTISYPK AIRKE L+DGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVTAADALSKFYYPPVASVTISYPKEAIRKERLVDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGG+TNTEIL++TESELV+AVDRDLRKI+IN NAEDPLLLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGSTNTEILAKTESELVEAVDRDLRKIIINTNAEDPLLLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLD+LDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE AA++A
Sbjct: 481 RVWPQAIPQFLIGHLDLLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEIA 540

Query: 541 GFLSQK 546
           GFLS+K
Sbjct: 541 GFLSKK 546

BLAST of MS018759 vs. ExPASy TrEMBL
Match: A0A6J1JZ95 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111489213 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.6e-285
Identity = 492/545 (90.28%), Postives = 514/545 (94.31%), Query Frame = 0

Query: 1   MATGATLLIDLPLRRTHRPSLLRPSNIPSFHPLHISLRSNHVRSNFRCSIAEGS-TLSAS 60
           MA+GATLL DLP RR + P+L RPSNIPSFHPLHIS+R NH+R NFRCSIAEGS T+S S
Sbjct: 1   MASGATLLTDLPFRRPYPPTLFRPSNIPSFHPLHISIRINHLRPNFRCSIAEGSATVSTS 60

Query: 61  KGTGPSSMVDCVVVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYL 120
             +G SS VDCVVVG GISGLCIAQALAT+HPDVAPNI+VTEA+ RVGGNITTVERDGYL
Sbjct: 61  DVSGQSSRVDCVVVGGGISGLCIAQALATRHPDVAPNIIVTEAKARVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLM 180
           WEEGPNSFQPSDP+LTMVVDSGLKDDLVLGDPDAPRFVLW GKLRPVP KP DLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPLLTMVVDSGLKDDLVLGDPDAPRFVLWKGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240
           S+GGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SLGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWKLEQNGGSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPT 300
           KAAFGKVWKLEQNGGSIIGGTFKA QERN ATKPPRDPRLPKPKGQTVGSFRKGLAMLP 
Sbjct: 241 KAAFGKVWKLEQNGGSIIGGTFKAFQERNKATKPPRDPRLPKPKGQTVGSFRKGLAMLPN 300

Query: 301 AISTCLGSKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRP 360
           AISTCLG+KVKLSWKLS++SKVDDGGY LTYETPEG VSILSRSVIMTVPSYVAS+LLRP
Sbjct: 301 AISTCLGNKVKLSWKLSNISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYVASTLLRP 360

Query: 361 ISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISV AAD LS+FYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISVKAADVLSEFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTEILS+TESELV+ VDRDLRKILIN NAEDP+LLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTEILSKTESELVEVVDRDLRKILINTNAEDPMLLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVA 540
           RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYV GVALGRCVEGAYE A +VA
Sbjct: 481 RVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVSGVALGRCVEGAYEAATEVA 540

Query: 541 GFLSQ 545
            FLS+
Sbjct: 541 SFLSK 545

BLAST of MS018759 vs. TAIR 10
Match: AT4G01690.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 819.7 bits (2116), Expect = 1.6e-237
Identity = 411/531 (77.40%), Postives = 458/531 (86.25%), Query Frame = 0

Query: 20  SLLRP---SNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASK---GTGPSSMVDCVVV 79
           SLLRP   S +PSF   ++ L + +     RCS+A G T+ +SK   G G +   DCV+V
Sbjct: 4   SLLRPTTQSLLPSFSKPNLRL-NVYKPLRLRCSVAGGPTVGSSKIEGGGGTTITTDCVIV 63

Query: 80  GAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSDPI 139
           G GISGLCIAQALATKHPD APN++VTEA+ RVGGNI T E +G+LWEEGPNSFQPSDP+
Sbjct: 64  GGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQPSDPM 123

Query: 140 LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALG 199
           LTMVVDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMSIGGKIRAGFGALG
Sbjct: 124 LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAGFGALG 183

Query: 200 IRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 259
           IRP PPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG
Sbjct: 184 IRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 243

Query: 260 GSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSW 319
           GSIIGGTFKA+QER NA K  RDPRLPKP+GQTVGSFRKGL MLP AIS  LGSKVKLSW
Sbjct: 244 GSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSKVKLSW 303

Query: 320 KLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYY 379
           KLS ++K++ GGY LTYETP+G VS+ S+SV+MTVPS+VAS LLRP+S +AA+ALSK YY
Sbjct: 304 KLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANALSKLYY 363

Query: 380 PPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVL 439
           PPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP GR+L
Sbjct: 364 PPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAPPGRIL 423

Query: 440 LLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGH 499
           LLNYIGG+TNT ILS++E ELV+AVDRDLRK+LI PN+ DPL L VRVWPQAIPQFL+GH
Sbjct: 424 LLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVRVWPQAIPQFLVGH 483

Query: 500 LDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQ 545
            D+LD AK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A +V  F+S+
Sbjct: 484 FDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFMSR 533

BLAST of MS018759 vs. TAIR 10
Match: AT4G01690.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 752.7 bits (1942), Expect = 2.4e-217
Identity = 386/531 (72.69%), Postives = 431/531 (81.17%), Query Frame = 0

Query: 20  SLLRP---SNIPSFHPLHISLRSNHVRSNFRCSIAEGSTLSASK---GTGPSSMVDCVVV 79
           SLLRP   S +PSF   ++ L + +     RCS+A G T+ +SK   G G +   DCV+V
Sbjct: 4   SLLRPTTQSLLPSFSKPNLRL-NVYKPLRLRCSVAGGPTVGSSKIEGGGGTTITTDCVIV 63

Query: 80  GAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSDPI 139
           G GISGLCIAQALATKHPD APN++VTEA+ RVGGNI T E +G+LWEEGPNSFQPSDP+
Sbjct: 64  GGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQPSDPM 123

Query: 140 LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFGALG 199
           LTMVVDSGLKDDLVLGDP APRFVLWNGKLRPVP K TDLPFFDLMSIGGKIRAGFGALG
Sbjct: 124 LTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAGFGALG 183

Query: 200 IRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 259
           IRP PPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG
Sbjct: 184 IRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNG 243

Query: 260 GSIIGGTFKALQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLGSKVKLSW 319
           GSIIGGTFKA+QER NA K  RDPRLPKP+GQTVGSFRKGL MLP AIS  LGSKVKLSW
Sbjct: 244 GSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSKVKLSW 303

Query: 320 KLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVPSYVASSLLRPISVTAADALSKFYY 379
           KLS ++K++ GGY LTYETP+G VS+ S+SV+MTVPS+VAS LLRP+S +AA+ALSK YY
Sbjct: 304 KLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANALSKLYY 363

Query: 380 PPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVL 439
           PPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP GR+L
Sbjct: 364 PPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAPPGRIL 423

Query: 440 LLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLLSVRVWPQAIPQFLIGH 499
           LLNYIGG+TNT ILS++E ELV+A                               FL+GH
Sbjct: 424 LLNYIGGSTNTGILSKSEGELVEA-------------------------------FLVGH 483

Query: 500 LDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQVAGFLSQ 545
            D+LD AK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A +V  F+S+
Sbjct: 484 FDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFMSR 502

BLAST of MS018759 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-43
Identity = 135/490 (27.55%), Postives = 234/490 (47.76%), Query Frame = 0

Query: 72  VVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSD 131
           VVGAG+SGL  A  L ++      N+ V EA GRVGG + +V ++G +W+EG N+   ++
Sbjct: 21  VVGAGVSGLAAAYKLKSR----GLNVTVFEADGRVGGKLRSVMQNGLIWDEGANTMTEAE 80

Query: 132 P-ILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFG 191
           P + +++ D GL++          R+++ NG    +P  P +L    ++S   K +    
Sbjct: 81  PEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVMLPTNPIELVTSSVLSTQSKFQILLE 140

Query: 192 AL-----GIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGK 251
                    +      EESV EF +R+ G EV + LI+PF  G  A DP  LSMK +F  
Sbjct: 141 PFLWKKKSSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGGTSAADPDSLSMKHSFPD 200

Query: 252 VWKLEQNGGSIIGGTFKA-LQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTC 311
           +W +E++ GSII G  +     +   ++  +     K   +   SF+ G+ +LP  +   
Sbjct: 201 LWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRGSFSFKGGMQILPDTLCKS 260

Query: 312 LG-------SKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVP--SYVASS 371
           L        SKV LS   +S S+ ++              +    +VIMT P  +     
Sbjct: 261 LSHDEINLDSKV-LSLSYNSGSRQENWSLSCVSHNETQRQNPHYDAVIMTAPLCNVKEMK 320

Query: 372 LLRPISVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHP---RSQGVT 431
           +++       + L +  Y P++ +  ++ K  +++       L+GFG L P   +  G  
Sbjct: 321 VMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKR------PLEGFGVLIPSKEQKHGFK 380

Query: 432 TLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAE 491
           TLGT++SS +FP+R+P    L   +IGG+ N E+   +  EL   V  DL+++L      
Sbjct: 381 TLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSDLQRLL--GVEG 440

Query: 492 DPLLLSVRVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAY 543
           +P+ ++   W +A P +   +  V++A      E  + G F  GN+  G+++G+ +    
Sbjct: 441 EPVSVNHYYWRKAFPLYDSSYDSVMEAIDK--MENDLPGFFYAGNHRGGLSVGKSIASGC 495

BLAST of MS018759 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 166.0 bits (419), Expect = 9.6e-41
Identity = 134/485 (27.63%), Postives = 229/485 (47.22%), Query Frame = 0

Query: 72  VVGAGISGLCIAQALATKHPDVAPNILVTEARGRVGGNITTVERDGYLWEEGPNSFQPSD 131
           VVGAG+SGL  A  L ++      N+ V EA GRVGG + +V ++G +W+EG N+   ++
Sbjct: 21  VVGAGVSGLAAAYKLKSR----GLNVTVFEADGRVGGKLRSVMQNGLIWDEGANTMTEAE 80

Query: 132 P-ILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGGKIRAGFG 191
           P + +++ D GL++          R+++ NG    VP          +M    K+     
Sbjct: 81  PEVGSLLDDLGLREKQQFPISQKKRYIVRNG----VP----------VMKKSSKV----- 140

Query: 192 ALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLE 251
                      EESV EF +R+ G EV + LI+PF  G  A DP  LSMK +F  +W +E
Sbjct: 141 ------SDASAEESVSEFFQRHFGQEVVDYLIDPFVGGTSAADPDSLSMKHSFPDLWNVE 200

Query: 252 QNGGSIIGGTFKA-LQERNNATKPPRDPRLPKPKGQTVGSFRKGLAMLPTAISTCLG--- 311
           ++ GSII G  +     +   ++  +     K   +   SF+ G+ +LP  +   L    
Sbjct: 201 KSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRGSFSFKGGMQILPDTLCKSLSHDE 260

Query: 312 ----SKVKLSWKLSSVSKVDDGGYCLTYETPEGPVSILSRSVIMTVP--SYVASSLLRPI 371
               SKV LS   +S S+ ++              +    +VIMT P  +     +++  
Sbjct: 261 INLDSKV-LSLSYNSGSRQENWSLSCVSHNETQRQNPHYDAVIMTAPLCNVKEMKVMKGG 320

Query: 372 SVTAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHP---RSQGVTTLGTI 431
                + L +  Y P++ +  ++ K  +++       L+GFG L P   +  G  TLGT+
Sbjct: 321 QPFQLNFLPEINYMPLSVLITTFTKEKVKR------PLEGFGVLIPSKEQKHGFKTLGTL 380

Query: 432 YSSSLFPNRAPDGRVLLLNYIGGATNTEILSQTESELVDAVDRDLRKILINPNAEDPLLL 491
           +SS +FP+R+P    L   +IGG+ N E+   +  EL   V  DL+++L      +P+ +
Sbjct: 381 FSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSDLQRLL--GVEGEPVSV 440

Query: 492 SVRVWPQAIPQFLIGHLDVLDAAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEVAAQ 543
           +   W +A P +   +  V++A      E  + G F  GN+  G+++G+ +    + A  
Sbjct: 441 NHYYWRKAFPLYDSSYDSVMEAIDK--MENDLPGFFYAGNHRGGLSVGKSIASGCKAADL 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146407.10.0e+0099.27protoporphyrinogen oxidase 1, chloroplastic [Momordica charantia][more]
XP_038875429.11.3e-28991.58protoporphyrinogen oxidase 1, chloroplastic [Benincasa hispida][more]
XP_023547363.13.5e-28791.03protoporphyrinogen oxidase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022990612.12.3e-28690.84protoporphyrinogen oxidase 1, chloroplastic-like [Cucurbita maxima][more]
XP_004149479.12.3e-28690.84protoporphyrinogen oxidase 1, chloroplastic [Cucumis sativus] >KGN49787.1 hypoth... [more]
Match NameE-valueIdentityDescription
P558262.3e-23677.40Protoporphyrinogen oxidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
O241631.2e-22674.76Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI P... [more]
Q9AR383.2e-22274.85Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=399... [more]
Q9LRI84.7e-21868.67Protoporphyrinogen oxidase 1, chloroplastic OS=Spinacia oleracea OX=3562 GN=POX1... [more]
P566013.7e-5332.35Coproporphyrinogen III oxidase OS=Myxococcus xanthus OX=34 GN=cgoX PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CZI00.0e+0099.27Protoporphyrinogen oxidase OS=Momordica charantia OX=3673 GN=LOC111015630 PE=3 S... [more]
A0A6J1JJ971.1e-28690.84Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111487440 PE=3 SV=1[more]
A0A0A0KMZ21.1e-28690.84Protoporphyrinogen oxidase OS=Cucumis sativus OX=3659 GN=Csa_5G128250 PE=3 SV=1[more]
A0A6J1HHU47.2e-28690.48Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463722 PE=3 SV... [more]
A0A6J1JZ953.6e-28590.28Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111489213 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G01690.11.6e-23777.40Flavin containing amine oxidoreductase family [more]
AT4G01690.22.4e-21772.69Flavin containing amine oxidoreductase family [more]
AT5G14220.11.2e-4327.55Flavin containing amine oxidoreductase family [more]
AT5G14220.29.6e-4127.63Flavin containing amine oxidoreductase family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002937Amine oxidasePFAMPF01593Amino_oxidasecoord: 77..539
e-value: 3.4E-86
score: 290.3
NoneNo IPR availableGENE3D3.90.660.20Protoporphyrinogen oxidase, mitochondrial; domain 2coord: 109..491
e-value: 1.5E-115
score: 388.9
NoneNo IPR availableGENE3D1.10.3110.10protoporphyrinogen ix oxidase, domain 3coord: 166..266
e-value: 1.5E-115
score: 388.9
NoneNo IPR availablePANTHERPTHR42923:SF3PROTOPORPHYRINOGEN OXIDASEcoord: 66..544
NoneNo IPR availablePANTHERPTHR42923PROTOPORPHYRINOGEN OXIDASEcoord: 66..544
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 372..488
IPR004572Protoporphyrinogen oxidaseTIGRFAMTIGR00562TIGR00562coord: 67..543
e-value: 6.4E-181
score: 600.0
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 71..535
e-value: 1.5E-115
score: 388.9
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 69..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018759.1MS018759.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006779 porphyrin-containing compound biosynthetic process
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0004729 oxygen-dependent protoporphyrinogen oxidase activity