MS018738 (gene) Bitter gourd (TR) v1

Overview
NameMS018738
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box/kelch-repeat protein At4g19930-like
Locationscaffold313: 1598152 .. 1600187 (-)
RNA-Seq ExpressionMS018738
SyntenyMS018738
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCGATGATGATCATCAACATCATCTTGTTCTTCTCCCACGACTCCCCGAAGACATCATAATCTTCCACATCCTCACGAAACTCCCCACGAAATCTCTCCTCCGTTTTCGCTCCGTCTCCAAATCATGGAACTCCGCCACCTCCCATCCTCTCTTCCTCTCTCTCCGCCACCTCCGTCCTCGATTCCTCTCCCACGTCCTCATCTCGGCCTCCCAACCCAACTCCTCCTGCCACCACCTCCTCTCCGCCGCCCTCGGCCCTCTCTCTCCGCTCACACACCTCCTCTCCCTTCCTCTCCGCCACTCCCTCGCCGTCAAACCCGCCAACGGCCTCCTCCTACTCCACTTCCCAAACGACCTCTACTCCGACCGCCCCGATCTTCCGGCCTACATCTTCAACCCCCGTGCCCGAGACATCATCCCTCTCCCTCCCAATGCGCCCACGAACCAGAAAGTTAAAATCTTGTACCATCTTGGTTACGATGCGTTGGGCGACGAGTACAAGGTTTTGCAGATTTGGAGGTTTATTGACACCAATCTTGAGATGTCGGAGAGTGGCATGGTTTTTAAAGTGTTGAGTTTGGGGTCTGGGAAATGGAGGCGGATTCGGCCTCGTCGGATCCCGTTCTTCTGTAGTTCTTATTGTGCTTTGTTTTCTCCTTGCTGTGCCTCCAATGGCGGAATCCACTGGGTCGTTCACCCCAAGAAATTTGTCTTGGTCTTCAATCTCCACGATGAGGAGTTTGCTTCCATTCCGTTTCCTCAACCATTCATCAACAACAAAGGTATAAACTTTAACAATCCCCTCTTCATTAAAGATTCATTTTTGGTTGGATAATAAATTTATCATTAGACAAAATTAAAAGTTTTAGGGTTTATAGATAACTATTTCGTTTTTTTTTAATAGTAGAAAAATTAATTTAAGAAACATTTGCTAAATAGAGCCTTAGATATTTGAAAAAGTAAAAAAATGTGAGGGAAGAATATATTGTTTTACATTTTTTGAATTACTTCTACCACACTATTTAAAGTTTAGAGATCTAATATGCATATTTTTGTCAACGGGAATAAATTAGCTATAATGGAAGGTTGGAGGTTCAAACCTTTATATATCTCCAATTTACGTTTTACTAAAAAAATAATAGACATACTTTTTAAATTCACCCAACTAGATTTATATATTAGACGCAATTCTTAAAAGTCTCGTTAGAAACCAAATTTGTAATTTAACCAGACATTTTTATCAAGATTTCTATACTTAAACTAATTATTTGGTGCCATTTAATTTCTATACTCTATCAAGATTCTAATTAGGTACTTAAGTTTGTCGTGTCTGGTCTAATATATTTTACTCGGTTAAATTCATTAATTTAATTGTTTTCAGACCGGTCACATTAAAATCAACACACCAAGTAATCTTTTAATTAACAAAATTGACACCATATACTGGTTTATATTATAATTTTATTTTAAAGTTAAAGACCTAACAGATACAATATAAAAAAGTTGAGGGAAAGAGAGATTAATACCCTAAAACTAAAACTTGGTTTTGGGAGTTAAAAAGAAACAAAAAACAAGCGTGTAAACTCAAATATAAAAAACTGCCATGCAGTTCACATTGAGGAAGAACATGTGGTGGAAGTTGGGACGTGTTTGGCACTTGTTCTAAAAACCAGTTCTTCGGATCAGATGGAGATGTGGGTGTTGAAGGATTACCAGAACCACATCTGGGTTCAACTTAAGGAAGCCACTTTCCAATACGACATGTCGTCTGAAGATGATGACCACTGCTCCTCTCGTCTCCCTCTTCCTCTGGGCTCTGTGAGTGATGGCGATGAAGAGATTTTAGTGTTCAAATCCATGGGGAAACAGGGACCACGGCTGCTCTACTATGACATCAAACGGCGCCGTTTTCGTGGAGTGGAGATTCGTGGGCTGTCTGAATGGCTGTCGGCTAATGATGTTGCTGTTACAGATTGTAGTTTTGATGCTTACGAAGAGAATATTGTCGCTCTGTCCTCTCTAATTGCT

mRNA sequence

ATGGCCTCCGATGATGATCATCAACATCATCTTGTTCTTCTCCCACGACTCCCCGAAGACATCATAATCTTCCACATCCTCACGAAACTCCCCACGAAATCTCTCCTCCGTTTTCGCTCCGTCTCCAAATCATGGAACTCCGCCACCTCCCATCCTCTCTTCCTCTCTCTCCGCCACCTCCGTCCTCGATTCCTCTCCCACGTCCTCATCTCGGCCTCCCAACCCAACTCCTCCTGCCACCACCTCCTCTCCGCCGCCCTCGGCCCTCTCTCTCCGCTCACACACCTCCTCTCCCTTCCTCTCCGCCACTCCCTCGCCGTCAAACCCGCCAACGGCCTCCTCCTACTCCACTTCCCAAACGACCTCTACTCCGACCGCCCCGATCTTCCGGCCTACATCTTCAACCCCCGTGCCCGAGACATCATCCCTCTCCCTCCCAATGCGCCCACGAACCAGAAAGTTAAAATCTTGTACCATCTTGGTTACGATGCGTTGGGCGACGAGTACAAGGTTTTGCAGATTTGGAGGTTTATTGACACCAATCTTGAGATGTCGGAGAGTGGCATGGTTTTTAAAGTGTTGAGTTTGGGGTCTGGGAAATGGAGGCGGATTCGGCCTCGTCGGATCCCGTTCTTCTGTAGTTCTTATTGTGCTTTGTTTTCTCCTTGCTGTGCCTCCAATGGCGGAATCCACTGGGTCGTTCACCCCAAGAAATTTGTCTTGGTCTTCAATCTCCACGATGAGGAGTTTGCTTCCATTCCGTTTCCTCAACCATTCATCAACAACAAAGTTCACATTGAGGAAGAACATGTGGTGGAAGTTGGGACGTGTTTGGCACTTGTTCTAAAAACCAGTTCTTCGGATCAGATGGAGATGTGGGTGTTGAAGGATTACCAGAACCACATCTGGGTTCAACTTAAGGAAGCCACTTTCCAATACGACATGTCGTCTGAAGATGATGACCACTGCTCCTCTCGTCTCCCTCTTCCTCTGGGCTCTGTGAGTGATGGCGATGAAGAGATTTTAGTGTTCAAATCCATGGGGAAACAGGGACCACGGCTGCTCTACTATGACATCAAACGGCGCCGTTTTCGTGGAGTGGAGATTCGTGGGCTGTCTGAATGGCTGTCGGCTAATGATGTTGCTGTTACAGATTGTAGTTTTGATGCTTACGAAGAGAATATTGTCGCTCTGTCCTCTCTAATTGCT

Coding sequence (CDS)

ATGGCCTCCGATGATGATCATCAACATCATCTTGTTCTTCTCCCACGACTCCCCGAAGACATCATAATCTTCCACATCCTCACGAAACTCCCCACGAAATCTCTCCTCCGTTTTCGCTCCGTCTCCAAATCATGGAACTCCGCCACCTCCCATCCTCTCTTCCTCTCTCTCCGCCACCTCCGTCCTCGATTCCTCTCCCACGTCCTCATCTCGGCCTCCCAACCCAACTCCTCCTGCCACCACCTCCTCTCCGCCGCCCTCGGCCCTCTCTCTCCGCTCACACACCTCCTCTCCCTTCCTCTCCGCCACTCCCTCGCCGTCAAACCCGCCAACGGCCTCCTCCTACTCCACTTCCCAAACGACCTCTACTCCGACCGCCCCGATCTTCCGGCCTACATCTTCAACCCCCGTGCCCGAGACATCATCCCTCTCCCTCCCAATGCGCCCACGAACCAGAAAGTTAAAATCTTGTACCATCTTGGTTACGATGCGTTGGGCGACGAGTACAAGGTTTTGCAGATTTGGAGGTTTATTGACACCAATCTTGAGATGTCGGAGAGTGGCATGGTTTTTAAAGTGTTGAGTTTGGGGTCTGGGAAATGGAGGCGGATTCGGCCTCGTCGGATCCCGTTCTTCTGTAGTTCTTATTGTGCTTTGTTTTCTCCTTGCTGTGCCTCCAATGGCGGAATCCACTGGGTCGTTCACCCCAAGAAATTTGTCTTGGTCTTCAATCTCCACGATGAGGAGTTTGCTTCCATTCCGTTTCCTCAACCATTCATCAACAACAAAGTTCACATTGAGGAAGAACATGTGGTGGAAGTTGGGACGTGTTTGGCACTTGTTCTAAAAACCAGTTCTTCGGATCAGATGGAGATGTGGGTGTTGAAGGATTACCAGAACCACATCTGGGTTCAACTTAAGGAAGCCACTTTCCAATACGACATGTCGTCTGAAGATGATGACCACTGCTCCTCTCGTCTCCCTCTTCCTCTGGGCTCTGTGAGTGATGGCGATGAAGAGATTTTAGTGTTCAAATCCATGGGGAAACAGGGACCACGGCTGCTCTACTATGACATCAAACGGCGCCGTTTTCGTGGAGTGGAGATTCGTGGGCTGTCTGAATGGCTGTCGGCTAATGATGTTGCTGTTACAGATTGTAGTTTTGATGCTTACGAAGAGAATATTGTCGCTCTGTCCTCTCTAATTGCT

Protein sequence

MASDDDHQHHLVLLPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISASQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHPKKFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQGPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVALSSLIA
Homology
BLAST of MS018738 vs. NCBI nr
Match: XP_022146376.1 (F-box/kelch-repeat protein At4g19930-like [Momordica charantia])

HSP 1 Score: 587.4 bits (1513), Expect = 9.3e-164
Identity = 296/355 (83.38%), Postives = 300/355 (84.51%), Query Frame = 0

Query: 49  TSHPLFLSLRHLRPRFLSHVLISASQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVK 108
           +S PL   LR LRPRFLSH+LISASQPNSSCHHLLSAALGPL PLTHLLSLPLRHSL VK
Sbjct: 8   SSLPLSPPLRRLRPRFLSHILISASQPNSSCHHLLSAALGPLPPLTHLLSLPLRHSLVVK 67

Query: 109 PANGLLLLHFPNDLYSDRPDLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDE 168
           PANGLLLLHFPNDLYSDRPDLPAYIFN RARDIIPLPPNAPTNQKVKILYHLGYDALGDE
Sbjct: 68  PANGLLLLHFPNDLYSDRPDLPAYIFNRRARDIIPLPPNAPTNQKVKILYHLGYDALGDE 127

Query: 169 YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNG 228
           YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSY ALFSPCCASNG
Sbjct: 128 YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYSALFSPCCASNG 187

Query: 229 GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSD 288
           GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFI  +                         
Sbjct: 188 GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFIQQQ------------------------S 247

Query: 289 QMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMG 348
            +EMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGS               
Sbjct: 248 PIEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGS--------------- 307

Query: 349 KQGPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVALSSLIA 404
             GPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIV LSSLIA
Sbjct: 308 --GPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVTLSSLIA 321

BLAST of MS018738 vs. NCBI nr
Match: XP_008385265.2 (F-box protein At4g19940-like [Malus domestica] >XP_017191226.2 F-box protein At4g19940-like [Malus domestica])

HSP 1 Score: 182.2 bits (461), Expect = 9.0e-42
Identity = 133/414 (32.13%), Postives = 208/414 (50.24%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II F IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 8   LPSEIIWFQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWDR 67

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPL--RHSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   GP +P  HLL+LP+   H L   +  NGL+ L+  N+L       
Sbjct: 68  ATRQQHFLSAQINQDGPPTPAIHLLNLPMGSDHRLYNAQSTNGLVCLYLSNNLSRHHTLA 127

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +P      + YH G+  L +EYKVLQ+  F   +   +
Sbjct: 128 YLDPPNFIFNPCTRESIILPHASPAYCTSHVTYHFGFSPLTNEYKVLQVQTFRPHDSIPT 187

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+  LG+  WRR+          P + PF   S C         NG +HW+   
Sbjct: 188 DMTFMFKIFKLGTSLWRRLEIDRNDLPFEPLKCPFQVQSVCI--------NGAMHWMHLK 247

Query: 257 KKFVLVFNLHDEEFASIPFPQPF------INNKVHIEEEHVVEVGTCLALV---LKTSSS 316
           +  V+VF++ DE+F  IP PQ +      I   +     +VVE+G CLAL+       S 
Sbjct: 248 ENVVVVFDIGDEKFRVIPLPQDYVCFIHRIGYPMGDAMRNVVEMGGCLALIGDKKLVRSQ 307

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G+  +   +  
Sbjct: 308 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNGELLLQSIELH 367

Query: 377 GKQGP---RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVAL 399
             + P   R+  Y+++ + FRG EI GL  W   ND  +       Y++NIV L
Sbjct: 368 VHEAPDHVRVHVYNMESKSFRGSEI-GL-PWAFLNDARLK--LVVCYDDNIVPL 401

BLAST of MS018738 vs. NCBI nr
Match: TQD90884.1 (hypothetical protein C1H46_023563 [Malus baccata])

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-41
Identity = 133/414 (32.13%), Postives = 208/414 (50.24%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II F IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 8   LPSEIIWFQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWDR 67

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPL--RHSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N+L       
Sbjct: 68  ATRQQHFLSAQINQDGTPTPAIHLLNLPMGSDHRLYNAQSTNGLVCLYLSNNLSRHHTLA 127

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +P      + YH G+  L +EYKVLQ+  F   +   +
Sbjct: 128 YLDPPNFIFNPCTRESIILPHASPAYCTSHVTYHFGFSPLTNEYKVLQVQTFRPHDSIPT 187

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+  LG+  WRR+          P + PF   S C         NG +HW+   
Sbjct: 188 DMTFMFKIFKLGTSLWRRLEIDRNDLPFEPLKCPFEVHSVCI--------NGAMHWMHLK 247

Query: 257 KKFVLVFNLHDEEFASIPFPQPFINNKVHI------EEEHVVEVGTCLALV---LKTSSS 316
           +  V+VF++ DE+F  IP PQ +++    I         +VVEVG CLAL+       S 
Sbjct: 248 ENVVVVFDIGDEKFRVIPLPQDYVSFIHRIGYPMGDAMRNVVEVGGCLALIGDKKLVRSQ 307

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G+  +   +  
Sbjct: 308 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNGELLLQSIELH 367

Query: 377 GKQGP---RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVAL 399
             + P   R+  Y+++ + FRG EI GL  W   ND  +       Y++NIV L
Sbjct: 368 VHEAPDHVRVHVYNMESKSFRGSEI-GL-PWTFLNDARLK--LVVCYDDNIVPL 401

BLAST of MS018738 vs. NCBI nr
Match: XP_009346574.1 (PREDICTED: putative F-box protein At5g62660 [Pyrus x bretschneideri] >KAB2606747.1 F-box protein [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-41
Identity = 136/415 (32.77%), Postives = 208/415 (50.12%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II   IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 8   LPNEIICCQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWNK 67

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPLR--HSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N         
Sbjct: 68  ATRQQHFLSAQINQDGTPTPAIHLLNLPMASDHRLYNAQSTNGLVCLYLSNTSSRHHTLA 127

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +PT     + YH GY  L +EYKVLQ+  F   +   +
Sbjct: 128 YLDPPNFIFNPCTRESIILPHASPTYCTSHVTYHFGYSPLTNEYKVLQVQTFRPHHSIPT 187

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+L LG+  WRR+          P + PF   S C         +G +HW+   
Sbjct: 188 DMTFMFKILKLGTSLWRRLEVDRNDLPFEPLQCPFEVQSVCI--------SGAMHWMHLK 247

Query: 257 KKFVLVFNLHDEEFASIPFPQPF------INNKVHIEEEHVVEVGTCLALV---LKTSSS 316
           +K V+VF++ DE+F  IP PQ +      I   +     +VVEVG CLAL+       S 
Sbjct: 248 EKVVVVFDIGDEKFRVIPLPQDYVCFIHRIGYPMGESMRNVVEVGGCLALIGDKTLVRSQ 307

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G E +L    +
Sbjct: 308 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNG-ELLLQSTEL 367

Query: 377 GKQGP----RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVAL 399
               P    R+  Y+++ + FRG EI GL  W   ND  +      +Y++NIV L
Sbjct: 368 HVHEPPDHVRVHVYNMESKSFRGSEI-GL-PWTFLNDARLK--LVVSYDDNIVPL 401

BLAST of MS018738 vs. NCBI nr
Match: RXH83739.1 (hypothetical protein DVH24_005992 [Malus domestica])

HSP 1 Score: 179.5 bits (454), Expect = 5.8e-41
Identity = 130/405 (32.10%), Postives = 202/405 (49.88%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II F IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 523 LPSEIIWFQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWDR 582

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPL--RHSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N+L       
Sbjct: 583 ATRQQHFLSAQINQDGTPTPAIHLLNLPMGSDHRLYNAQSTNGLVCLYLSNNLSRHHTLA 642

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +P      + YH G+  L +EYKVLQ+  F   +   +
Sbjct: 643 YLDPPNFIFNPCTRESIILPHASPAYCTSHVTYHFGFSPLTNEYKVLQVQTFRPHDSIPT 702

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+  LG+  WRR+          P + PF   S C         NG +HW+   
Sbjct: 703 DMTFMFKIFKLGTSLWRRLEIDRNDLPFEPLKCPFAVQSVCI--------NGAMHWMHLK 762

Query: 257 KKFVLVFNLHDEEFASIPFPQPFINNKVHI------EEEHVVEVGTCLALV---LKTSSS 316
           +  V+VF++ DE+F  IP PQ +++    I         +VVEVG CLAL+       S 
Sbjct: 763 ENVVVVFDIGDEKFRVIPLPQDYVSFIHRIGYPMGDAMRNVVEVGGCLALIGDKKLVRSQ 822

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G+  +   +  
Sbjct: 823 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNGELLLQSIELH 882

Query: 377 GKQGP---RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFD 390
             + P   R+  Y+++   FRG EI GL  W   ND  +   S D
Sbjct: 883 VHEAPDHVRVHVYNMESNSFRGSEI-GL-PWAFLNDARLKLVSMD 909

BLAST of MS018738 vs. ExPASy Swiss-Prot
Match: O49421 (F-box protein At4g19940 OS=Arabidopsis thaliana OX=3702 GN=At4g19940 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.3e-19
Identity = 103/380 (27.11%), Postives = 171/380 (45.00%), Query Frame = 0

Query: 14  LPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLS-LRHLRPRFLSHVLISA 73
           +P +P D++I  ILT+LP KSL+RF+SVSK W+S      F + L  L       + +S+
Sbjct: 32  IPEIPFDLVI-EILTRLPAKSLMRFKSVSKLWSSLICSRNFTNRLLKLSSPPRLFMCLSS 91

Query: 74  SQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLPAY 133
           S  +     LLS +  P S +T + S  +   L +    G  + H    L        A 
Sbjct: 92  SDNSHLKTVLLSLSSPPDSDIT-MSSSVIDQDLTMPGMKGYQISHVFRGLMCLVKKSSAQ 151

Query: 134 IFNPRARDIIPLPP------NAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLE--- 193
           I+N   R ++ LP        A  ++  KI+YH+G+D + D+YKV+ I       +E   
Sbjct: 152 IYNTTTRQLVVLPDIEESTILAEEHKSKKIMYHIGHDPVYDQYKVVCIVSRASDEVEEYT 211

Query: 194 -MSESGMVFKVLSLGSGKWRRIR---PRRIPFFCSSYCALFSPCCASNGGIH---WV-VH 253
            +SE   V  +   GS +WR+I    P  +P                +G +H   WV V 
Sbjct: 212 FLSEH-WVLLLEGEGSRRWRKISCKYPPHVP---------LGQGLTLSGRMHYLAWVRVS 271

Query: 254 PKKFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALV--LKTSSSDQMEMW 313
             + +++F+ H EEF+ +  P       +  +   ++E G  +A++   K      ME+W
Sbjct: 272 DNRVLVIFDTHSEEFSMLQVP-----GDIFWKYNGLLEYGGKIAILNYTKVDIEGVMELW 331

Query: 314 VLKDYQNHIWVQ--LKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQG 372
           V++D + ++W    L     Q  M        +S + L +   +   E ILV     K  
Sbjct: 332 VVEDEEKNLWSSKILVVNPLQLQM-------VNSIISLTVLGTTRNGEVILVPGPEDKTV 387

BLAST of MS018738 vs. ExPASy Swiss-Prot
Match: O49420 (F-box/kelch-repeat protein At4g19930 OS=Arabidopsis thaliana OX=3702 GN=At4g19930 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-18
Identity = 104/390 (26.67%), Postives = 175/390 (44.87%), Query Frame = 0

Query: 14  LPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISAS 73
           +P +P D++I  ILT+LP KSL+RF+SVSK W+S      F +     P F+  + ++ +
Sbjct: 40  MPYIPFDLVI-EILTRLPAKSLMRFKSVSKLWSSLICSRTFTNRLLRVPSFIQRLYVTLT 99

Query: 74  QPNSSCHH---LLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLP 133
             ++S      LLS++  P S ++ + S  +   L      G  L H    L     +  
Sbjct: 100 FLDNSLQRKSKLLSSSSSPGSDISTMSSFVVDRDLTTPSMKGYYLSHVLRGLMCFVKEPS 159

Query: 134 AYIFNPRARDIIPLPPNAPTN------QKVKILYHLGYDALGDEYKVLQIW-----RFID 193
             I+N   R ++ LP    +N      +  KI+Y +G+D +GD+YKV+ I       F +
Sbjct: 160 VKIYNTTTRQLVVLPDIEESNIIAEDHKNKKIMYRIGHDPVGDQYKVVCIVARPNDEFGE 219

Query: 194 TNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHPKKF 253
               +SE   VF +    S  WR+I        C S     +   + NG +H++   +KF
Sbjct: 220 LRRYLSEH-WVFILGGDKSSGWRKIP-------CPSPHLPITQILSINGRMHYLAWVQKF 279

Query: 254 ---VLVFNLHDEEFASIPFPQP---FINNKVHIEEEHVVEVGTCLALVLKTSSSDQMEMW 313
              ++ F+   EE + +  P+    F +N +    E+  +V       LK   +  M +W
Sbjct: 280 DPMLVTFDFSSEEISILQAPEDIRWFKSNPI----EYYGKVALLNLSDLKRECT--MNLW 339

Query: 314 VLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILV-----FKSMG 371
           V++D + ++W    E T     S  D    +S   L +   +  +E ILV     +   G
Sbjct: 340 VMEDVEKNMW---SEKTLVVHPSQMDIVKSTS---LRVAGTTRNNEVILVPHNIRYTLTG 399

BLAST of MS018738 vs. ExPASy Swiss-Prot
Match: Q9FJJ4 (F-box protein At5g62510 OS=Arabidopsis thaliana OX=3702 GN=At5g62510 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.1e-13
Identity = 107/411 (26.03%), Postives = 175/411 (42.58%), Query Frame = 0

Query: 15  PRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRH------LRPR----- 74
           P +P D++I  ILT+LP KSL+RF+ VSK W+S      F S R+      LRP      
Sbjct: 42  PEIPLDLLI-EILTRLPHKSLMRFKCVSKQWSSLI-RSRFFSNRYLTVASPLRPHRLYIS 101

Query: 75  FLSHVLISASQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLY 134
            + H   S    +S    +L +   P S    L ++     L +    GL+L        
Sbjct: 102 LVDHKCDSREVCHSPRESVLLSFSSPSSFDQDLTTMQGMGGLHMVTLRGLILYIVCG--- 161

Query: 135 SDRPDLPAYIFNPRARDIIPLPPNA----PTNQKVKILYHLGYDALGDEYKVLQIWRFID 194
                  A ++NP  R  + LP           +  +LY LG+D + D+YKV  +  F+ 
Sbjct: 162 ------KACLYNPTTRQSVTLPAIKFNIFVQGNEHSLLYFLGHDPVLDQYKV--VCTFVS 221

Query: 195 T---NLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 254
           +   +LE   S     VL +G G W+RI       F   +    S  C   G I+++   
Sbjct: 222 SSSQDLETIISEHWVFVLEVG-GSWKRIE------FDQPHTPTRSGLCI-GGVIYYLAFT 281

Query: 255 KKF---VLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQ--ME 314
             F   V+ F++  EEF  I  P   +    +++    +E G   A+   TS  +   ++
Sbjct: 282 SMFQDIVVTFDVRSEEFNIIQAP---LVLSAYVDSLDFIEYGGKPAIFYHTSLKENGLVD 341

Query: 315 MWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQG 374
           +WVL++  N  W +   +     +   D+D     +PL L   +   E +LV   +    
Sbjct: 342 LWVLENAGN--WSRTVLSLQPCQLHLVDND-----IPLTLVDTTQNGEVVLVPSDLCSPF 401

Query: 375 PRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYE--ENIVALSS 401
             +LYYDI++   R VEI+ + +        + D  F   +  E+I+ L +
Sbjct: 402 -YILYYDIQKNHLRKVEIQAMPDHKKDGIKGIPDFGFKLMDKSESIIHLET 420

BLAST of MS018738 vs. ExPASy Swiss-Prot
Match: Q9FT50 (Putative F-box protein At3g52320 OS=Arabidopsis thaliana OX=3702 GN=At3g52320 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.0e-12
Identity = 98/376 (26.06%), Postives = 161/376 (42.82%), Query Frame = 0

Query: 11  LVLLPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVL- 70
           +V LP +PE+++I  IL +LP KSL+RF+ VSK W S  +   F + R  +P   S +  
Sbjct: 21  VVFLPEIPEEMLI-DILIRLPAKSLMRFKCVSKLWLSLITSRYFTN-RFFKPSSPSCLFA 80

Query: 71  ISASQPNSSCHHLLSAALG--------PLSPLTHLLSLPLRHSLAVKPANGLLLLHFPND 130
               + N S + LL ++           +S +    ++P+     V  A GLL       
Sbjct: 81  YLVDRENQSKYLLLQSSSSSRHDHSDTSVSVIDQHSTIPIMGGYLVNAARGLLCY----- 140

Query: 131 LYSDRPDLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTN 190
               R      + NP  R I+ LP       K  +    G+D   DEYKVL +  F +  
Sbjct: 141 ----RTGRRVKVCNPSTRQIVELP---IMRSKTNVWNWFGHDPFHDEYKVLSL--FWEVT 200

Query: 191 LEMSESGMVFKVLSLGSG-KWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHP---K 250
            E +      +VL LG G  WR  +    P         +S     +G +++       +
Sbjct: 201 KEQTVVRSEHQVLVLGVGASWRNTKSHHTP---HRPFHPYSRGMTIDGVLYYSARTDANR 260

Query: 251 KFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQMEMWVLKD 310
             ++ F+L  EEF  I  P    +  +H+  +   +V TC    ++ +S   +++ VL+D
Sbjct: 261 CVLMSFDLSSEEFNLIELPFENWSRTIHMNYQG--KVATC--QYMRLASDGFVDVCVLED 320

Query: 311 YQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKS--MGKQGPRLL 370
                W   K  TF   +S  +  H      L +G+  D   ++L+ K+  +  Q  R  
Sbjct: 321 ADKSQWSNKK--TFVLPISQMNFVHGDR---LVVGASRDSG-KVLMRKANLLRNQHARFF 367

BLAST of MS018738 vs. ExPASy Swiss-Prot
Match: Q84X43 (F-box only protein 8 OS=Arabidopsis thaliana OX=3702 GN=FBX8 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.9e-12
Identity = 74/256 (28.91%), Postives = 119/256 (46.48%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISASQPN 76
           +P+D++I  ILT+LP KS++RF+ VSK W+S       LS R+   RF    LI  SQP 
Sbjct: 41  IPQDLLI-EILTRLPPKSVMRFKCVSKFWSS------LLSSRYFCNRF----LIVPSQPQ 100

Query: 77  SSCHHLL-------SAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDL 136
            S +  L        + +   +P T   S      L ++   G  L      ++  R +L
Sbjct: 101 PSLYMCLLDRYNYSKSLILSSAPSTSPYSFVFDQDLTIRKMGGFFLRILRGFIFFTR-NL 160

Query: 137 PAYIFNPRARDIIPLP----PNAPTNQKVKILYHLGYDALGDEYKVLQIWRFI-DTNLEM 196
            A I+NP  R ++ LP     +        ILY + +D + D YK+L    +  D +L+ 
Sbjct: 161 KARIYNPTTRQLVILPTIKESDIIAGPPYNILYFICHDPVNDRYKLLCTVSYASDNDLQN 220

Query: 197 SESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWV--VHPKKFVLV 256
            +S +   VL  G G W+R+     P    S+  L       NG ++++    P   +LV
Sbjct: 221 LKSELWIFVLEAG-GSWKRV-ANEFPHHVPSHLDL-----NMNGVLYFLAWTDPHTCMLV 277

Query: 257 -FNLHDEEFASIPFPQ 258
            F++  EEF ++  P+
Sbjct: 281 SFDVRSEEFNTMQVPR 277

BLAST of MS018738 vs. ExPASy TrEMBL
Match: A0A6J1CZ64 (F-box/kelch-repeat protein At4g19930-like OS=Momordica charantia OX=3673 GN=LOC111015605 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 4.5e-164
Identity = 296/355 (83.38%), Postives = 300/355 (84.51%), Query Frame = 0

Query: 49  TSHPLFLSLRHLRPRFLSHVLISASQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVK 108
           +S PL   LR LRPRFLSH+LISASQPNSSCHHLLSAALGPL PLTHLLSLPLRHSL VK
Sbjct: 8   SSLPLSPPLRRLRPRFLSHILISASQPNSSCHHLLSAALGPLPPLTHLLSLPLRHSLVVK 67

Query: 109 PANGLLLLHFPNDLYSDRPDLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDE 168
           PANGLLLLHFPNDLYSDRPDLPAYIFN RARDIIPLPPNAPTNQKVKILYHLGYDALGDE
Sbjct: 68  PANGLLLLHFPNDLYSDRPDLPAYIFNRRARDIIPLPPNAPTNQKVKILYHLGYDALGDE 127

Query: 169 YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNG 228
           YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSY ALFSPCCASNG
Sbjct: 128 YKVLQIWRFIDTNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYSALFSPCCASNG 187

Query: 229 GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSD 288
           GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFI  +                         
Sbjct: 188 GIHWVVHPKKFVLVFNLHDEEFASIPFPQPFIQQQ------------------------S 247

Query: 289 QMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMG 348
            +EMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGS               
Sbjct: 248 PIEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGS--------------- 307

Query: 349 KQGPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVALSSLIA 404
             GPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIV LSSLIA
Sbjct: 308 --GPRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVTLSSLIA 321

BLAST of MS018738 vs. ExPASy TrEMBL
Match: A0A2P2K4Y2 (F-box protein At5g62510-like OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.4e-45
Identity = 134/394 (34.01%), Postives = 201/394 (51.02%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISASQ-- 76
           LP DII  HIL +LP +SL+RFR VSK WN  T  P F  L H      +H+LI++    
Sbjct: 10  LPGDIIYTHILPRLPVRSLMRFRCVSKYWNQLTYDPSFARL-HKSLHCPTHLLITSWSVV 69

Query: 77  PNSSCHHLLSAAL---GPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLPA 136
            + S  H +S  +     L    HLLSLP+     ++  NGLL +H  N +Y    D P 
Sbjct: 70  GDESRIHFVSVPVRKDAKLGTPVHLLSLPIDKHYVLQSLNGLLCIHRSNMMYPYHGDKPV 129

Query: 137 YIFNPRARDIIPLP-PNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMSESGMV 196
           +IFNP  R+I  +P  +    +   + Y+ G+  L +E+KVL + RF   N++     + 
Sbjct: 130 FIFNPSTREIATVPVDDTLKTRSSHVSYYFGFCQLTNEHKVLGLRRFF--NMDFEPISIE 189

Query: 197 FKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHPKKFVLVFNLHDEEF 256
            +V ++GSG WRRI+P  +PF  +       P C  NG +HW+   +K ++ F++  E F
Sbjct: 190 CEVYTIGSGSWRRIKP-AVPFDPAELTYYTDPVC-FNGALHWICQSQKSIVAFDVGGECF 249

Query: 257 ASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQ--MEMWVLKDYQNHIWVQLKE 316
                P+ F +        +++ VG  LA+V      D+  ME+WVL DYQ H+WV+   
Sbjct: 250 REFQMPEGFPSRSF---LGYLLIVGGRLAMVNGKHLFDESTMELWVLDDYQKHVWVK--- 309

Query: 317 ATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQGPRLLYYDIKRRRFRGVE 376
                D      D   S  P+P G++ +G  E+L+   +      +L+YD+KRR FR VE
Sbjct: 310 -----DCIKFPFDWTESGRPIPAGTIHNG--ELLLLPILSVSSMSVLFYDMKRRSFRRVE 369

Query: 377 IRGLSEWLSANDVAVTDCSFDAYEENIVALSSLI 403
           + GL E        +T     +YEE+I+AL   I
Sbjct: 370 VSGLPEHFLPISQRIT-----SYEESIMALGGRI 380

BLAST of MS018738 vs. ExPASy TrEMBL
Match: A0A5N5FV35 (F-box protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_006464 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-41
Identity = 136/415 (32.77%), Postives = 208/415 (50.12%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II   IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 8   LPNEIICCQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWNK 67

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPLR--HSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N         
Sbjct: 68  ATRQQHFLSAQINQDGTPTPAIHLLNLPMASDHRLYNAQSTNGLVCLYLSNTSSRHHTLA 127

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +PT     + YH GY  L +EYKVLQ+  F   +   +
Sbjct: 128 YLDPPNFIFNPCTRESIILPHASPTYCTSHVTYHFGYSPLTNEYKVLQVQTFRPHHSIPT 187

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+L LG+  WRR+          P + PF   S C         +G +HW+   
Sbjct: 188 DMTFMFKILKLGTSLWRRLEVDRNDLPFEPLQCPFEVQSVCI--------SGAMHWMHLK 247

Query: 257 KKFVLVFNLHDEEFASIPFPQPF------INNKVHIEEEHVVEVGTCLALV---LKTSSS 316
           +K V+VF++ DE+F  IP PQ +      I   +     +VVEVG CLAL+       S 
Sbjct: 248 EKVVVVFDIGDEKFRVIPLPQDYVCFIHRIGYPMGESMRNVVEVGGCLALIGDKTLVRSQ 307

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G E +L    +
Sbjct: 308 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNG-ELLLQSTEL 367

Query: 377 GKQGP----RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVAL 399
               P    R+  Y+++ + FRG EI GL  W   ND  +      +Y++NIV L
Sbjct: 368 HVHEPPDHVRVHVYNMESKSFRGSEI-GL-PWTFLNDARLK--LVVSYDDNIVPL 401

BLAST of MS018738 vs. ExPASy TrEMBL
Match: A0A540LWM9 (F-box domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_023563 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-41
Identity = 133/414 (32.13%), Postives = 208/414 (50.24%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II F IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 8   LPSEIIWFQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWDR 67

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPL--RHSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N+L       
Sbjct: 68  ATRQQHFLSAQINQDGTPTPAIHLLNLPMGSDHRLYNAQSTNGLVCLYLSNNLSRHHTLA 127

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +P      + YH G+  L +EYKVLQ+  F   +   +
Sbjct: 128 YLDPPNFIFNPCTRESIILPHASPAYCTSHVTYHFGFSPLTNEYKVLQVQTFRPHDSIPT 187

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+  LG+  WRR+          P + PF   S C         NG +HW+   
Sbjct: 188 DMTFMFKIFKLGTSLWRRLEIDRNDLPFEPLKCPFEVHSVCI--------NGAMHWMHLK 247

Query: 257 KKFVLVFNLHDEEFASIPFPQPFINNKVHI------EEEHVVEVGTCLALV---LKTSSS 316
           +  V+VF++ DE+F  IP PQ +++    I         +VVEVG CLAL+       S 
Sbjct: 248 ENVVVVFDIGDEKFRVIPLPQDYVSFIHRIGYPMGDAMRNVVEVGGCLALIGDKKLVRSQ 307

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G+  +   +  
Sbjct: 308 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNGELLLQSIELH 367

Query: 377 GKQGP---RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYEENIVAL 399
             + P   R+  Y+++ + FRG EI GL  W   ND  +       Y++NIV L
Sbjct: 368 VHEAPDHVRVHVYNMESKSFRGSEI-GL-PWTFLNDARLK--LVVCYDDNIVPL 401

BLAST of MS018738 vs. ExPASy TrEMBL
Match: A0A498IKL3 (tRNA-intron lyase OS=Malus domestica OX=3750 GN=DVH24_005992 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 2.8e-41
Identity = 130/405 (32.10%), Postives = 202/405 (49.88%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSL-RHLRPRFLSHVLISASQP 76
           LP +II F IL ++P+KSL+RF+ V KSW+S   +P F+   +      L+H+L++    
Sbjct: 523 LPSEIIWFQILPRVPSKSLMRFKCVCKSWSSLVRNPSFVDAHQSFHRNKLTHLLLTTWDR 582

Query: 77  NSSCHHLLSAAL---GPLSPLTHLLSLPL--RHSL-AVKPANGLLLLHFPNDLYSDRP-- 136
            +   H LSA +   G  +P  HLL+LP+   H L   +  NGL+ L+  N+L       
Sbjct: 583 ATRQQHFLSAQINQDGTPTPAIHLLNLPMGSDHRLYNAQSTNGLVCLYLSNNLSRHHTLA 642

Query: 137 --DLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLEMS 196
             D P +IFNP  R+ I LP  +P      + YH G+  L +EYKVLQ+  F   +   +
Sbjct: 643 YLDPPNFIFNPCTRESIILPHASPAYCTSHVTYHFGFSPLTNEYKVLQVQTFRPHDSIPT 702

Query: 197 ESGMVFKVLSLGSGKWRRI---------RPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 256
           +   +FK+  LG+  WRR+          P + PF   S C         NG +HW+   
Sbjct: 703 DMTFMFKIFKLGTSLWRRLEIDRNDLPFEPLKCPFAVQSVCI--------NGAMHWMHLK 762

Query: 257 KKFVLVFNLHDEEFASIPFPQPFINNKVHI------EEEHVVEVGTCLALV---LKTSSS 316
           +  V+VF++ DE+F  IP PQ +++    I         +VVEVG CLAL+       S 
Sbjct: 763 ENVVVVFDIGDEKFRVIPLPQDYVSFIHRIGYPMGDAMRNVVEVGGCLALIGDKKLVRSQ 822

Query: 317 DQMEMWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSM 376
           + M +W+LKDY+N +W++ +  TF            +   P+PL ++ +G+  +   +  
Sbjct: 823 NMMALWILKDYENQVWIR-EAITFPLRWR-------ALGCPVPLRTIHNGELLLQSIELH 882

Query: 377 GKQGP---RLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFD 390
             + P   R+  Y+++   FRG EI GL  W   ND  +   S D
Sbjct: 883 VHEAPDHVRVHVYNMESNSFRGSEI-GL-PWAFLNDARLKLVSMD 909

BLAST of MS018738 vs. TAIR 10
Match: AT4G19940.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 99.0 bits (245), Expect = 9.3e-21
Identity = 103/380 (27.11%), Postives = 171/380 (45.00%), Query Frame = 0

Query: 14  LPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLS-LRHLRPRFLSHVLISA 73
           +P +P D++I  ILT+LP KSL+RF+SVSK W+S      F + L  L       + +S+
Sbjct: 32  IPEIPFDLVI-EILTRLPAKSLMRFKSVSKLWSSLICSRNFTNRLLKLSSPPRLFMCLSS 91

Query: 74  SQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLPAY 133
           S  +     LLS +  P S +T + S  +   L +    G  + H    L        A 
Sbjct: 92  SDNSHLKTVLLSLSSPPDSDIT-MSSSVIDQDLTMPGMKGYQISHVFRGLMCLVKKSSAQ 151

Query: 134 IFNPRARDIIPLPP------NAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTNLE--- 193
           I+N   R ++ LP        A  ++  KI+YH+G+D + D+YKV+ I       +E   
Sbjct: 152 IYNTTTRQLVVLPDIEESTILAEEHKSKKIMYHIGHDPVYDQYKVVCIVSRASDEVEEYT 211

Query: 194 -MSESGMVFKVLSLGSGKWRRIR---PRRIPFFCSSYCALFSPCCASNGGIH---WV-VH 253
            +SE   V  +   GS +WR+I    P  +P                +G +H   WV V 
Sbjct: 212 FLSEH-WVLLLEGEGSRRWRKISCKYPPHVP---------LGQGLTLSGRMHYLAWVRVS 271

Query: 254 PKKFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALV--LKTSSSDQMEMW 313
             + +++F+ H EEF+ +  P       +  +   ++E G  +A++   K      ME+W
Sbjct: 272 DNRVLVIFDTHSEEFSMLQVP-----GDIFWKYNGLLEYGGKIAILNYTKVDIEGVMELW 331

Query: 314 VLKDYQNHIWVQ--LKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQG 372
           V++D + ++W    L     Q  M        +S + L +   +   E ILV     K  
Sbjct: 332 VVEDEEKNLWSSKILVVNPLQLQM-------VNSIISLTVLGTTRNGEVILVPGPEDKTV 387

BLAST of MS018738 vs. TAIR 10
Match: AT4G19930.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 95.9 bits (237), Expect = 7.9e-20
Identity = 104/390 (26.67%), Postives = 175/390 (44.87%), Query Frame = 0

Query: 14  LPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISAS 73
           +P +P D++I  ILT+LP KSL+RF+SVSK W+S      F +     P F+  + ++ +
Sbjct: 40  MPYIPFDLVI-EILTRLPAKSLMRFKSVSKLWSSLICSRTFTNRLLRVPSFIQRLYVTLT 99

Query: 74  QPNSSCHH---LLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDLP 133
             ++S      LLS++  P S ++ + S  +   L      G  L H    L     +  
Sbjct: 100 FLDNSLQRKSKLLSSSSSPGSDISTMSSFVVDRDLTTPSMKGYYLSHVLRGLMCFVKEPS 159

Query: 134 AYIFNPRARDIIPLPPNAPTN------QKVKILYHLGYDALGDEYKVLQIW-----RFID 193
             I+N   R ++ LP    +N      +  KI+Y +G+D +GD+YKV+ I       F +
Sbjct: 160 VKIYNTTTRQLVVLPDIEESNIIAEDHKNKKIMYRIGHDPVGDQYKVVCIVARPNDEFGE 219

Query: 194 TNLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHPKKF 253
               +SE   VF +    S  WR+I        C S     +   + NG +H++   +KF
Sbjct: 220 LRRYLSEH-WVFILGGDKSSGWRKIP-------CPSPHLPITQILSINGRMHYLAWVQKF 279

Query: 254 ---VLVFNLHDEEFASIPFPQP---FINNKVHIEEEHVVEVGTCLALVLKTSSSDQMEMW 313
              ++ F+   EE + +  P+    F +N +    E+  +V       LK   +  M +W
Sbjct: 280 DPMLVTFDFSSEEISILQAPEDIRWFKSNPI----EYYGKVALLNLSDLKRECT--MNLW 339

Query: 314 VLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILV-----FKSMG 371
           V++D + ++W    E T     S  D    +S   L +   +  +E ILV     +   G
Sbjct: 340 VMEDVEKNMW---SEKTLVVHPSQMDIVKSTS---LRVAGTTRNNEVILVPHNIRYTLTG 399

BLAST of MS018738 vs. TAIR 10
Match: AT5G62510.1 (F-box family protein )

HSP 1 Score: 79.3 bits (194), Expect = 7.6e-15
Identity = 107/411 (26.03%), Postives = 175/411 (42.58%), Query Frame = 0

Query: 15  PRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRH------LRPR----- 74
           P +P D++I  ILT+LP KSL+RF+ VSK W+S      F S R+      LRP      
Sbjct: 42  PEIPLDLLI-EILTRLPHKSLMRFKCVSKQWSSLI-RSRFFSNRYLTVASPLRPHRLYIS 101

Query: 75  FLSHVLISASQPNSSCHHLLSAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLY 134
            + H   S    +S    +L +   P S    L ++     L +    GL+L        
Sbjct: 102 LVDHKCDSREVCHSPRESVLLSFSSPSSFDQDLTTMQGMGGLHMVTLRGLILYIVCG--- 161

Query: 135 SDRPDLPAYIFNPRARDIIPLPPNA----PTNQKVKILYHLGYDALGDEYKVLQIWRFID 194
                  A ++NP  R  + LP           +  +LY LG+D + D+YKV  +  F+ 
Sbjct: 162 ------KACLYNPTTRQSVTLPAIKFNIFVQGNEHSLLYFLGHDPVLDQYKV--VCTFVS 221

Query: 195 T---NLEMSESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHP 254
           +   +LE   S     VL +G G W+RI       F   +    S  C   G I+++   
Sbjct: 222 SSSQDLETIISEHWVFVLEVG-GSWKRIE------FDQPHTPTRSGLCI-GGVIYYLAFT 281

Query: 255 KKF---VLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQ--ME 314
             F   V+ F++  EEF  I  P   +    +++    +E G   A+   TS  +   ++
Sbjct: 282 SMFQDIVVTFDVRSEEFNIIQAP---LVLSAYVDSLDFIEYGGKPAIFYHTSLKENGLVD 341

Query: 315 MWVLKDYQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKSMGKQG 374
           +WVL++  N  W +   +     +   D+D     +PL L   +   E +LV   +    
Sbjct: 342 LWVLENAGN--WSRTVLSLQPCQLHLVDND-----IPLTLVDTTQNGEVVLVPSDLCSPF 401

Query: 375 PRLLYYDIKRRRFRGVEIRGLSEWLSANDVAVTDCSFDAYE--ENIVALSS 401
             +LYYDI++   R VEI+ + +        + D  F   +  E+I+ L +
Sbjct: 402 -YILYYDIQKNHLRKVEIQAMPDHKKDGIKGIPDFGFKLMDKSESIIHLET 420

BLAST of MS018738 vs. TAIR 10
Match: AT3G52320.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.4e-13
Identity = 98/376 (26.06%), Postives = 161/376 (42.82%), Query Frame = 0

Query: 11  LVLLPRLPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVL- 70
           +V LP +PE+++I  IL +LP KSL+RF+ VSK W S  +   F + R  +P   S +  
Sbjct: 21  VVFLPEIPEEMLI-DILIRLPAKSLMRFKCVSKLWLSLITSRYFTN-RFFKPSSPSCLFA 80

Query: 71  ISASQPNSSCHHLLSAALG--------PLSPLTHLLSLPLRHSLAVKPANGLLLLHFPND 130
               + N S + LL ++           +S +    ++P+     V  A GLL       
Sbjct: 81  YLVDRENQSKYLLLQSSSSSRHDHSDTSVSVIDQHSTIPIMGGYLVNAARGLLCY----- 140

Query: 131 LYSDRPDLPAYIFNPRARDIIPLPPNAPTNQKVKILYHLGYDALGDEYKVLQIWRFIDTN 190
               R      + NP  R I+ LP       K  +    G+D   DEYKVL +  F +  
Sbjct: 141 ----RTGRRVKVCNPSTRQIVELP---IMRSKTNVWNWFGHDPFHDEYKVLSL--FWEVT 200

Query: 191 LEMSESGMVFKVLSLGSG-KWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWVVHP---K 250
            E +      +VL LG G  WR  +    P         +S     +G +++       +
Sbjct: 201 KEQTVVRSEHQVLVLGVGASWRNTKSHHTP---HRPFHPYSRGMTIDGVLYYSARTDANR 260

Query: 251 KFVLVFNLHDEEFASIPFPQPFINNKVHIEEEHVVEVGTCLALVLKTSSSDQMEMWVLKD 310
             ++ F+L  EEF  I  P    +  +H+  +   +V TC    ++ +S   +++ VL+D
Sbjct: 261 CVLMSFDLSSEEFNLIELPFENWSRTIHMNYQG--KVATC--QYMRLASDGFVDVCVLED 320

Query: 311 YQNHIWVQLKEATFQYDMSSEDDDHCSSRLPLPLGSVSDGDEEILVFKS--MGKQGPRLL 370
                W   K  TF   +S  +  H      L +G+  D   ++L+ K+  +  Q  R  
Sbjct: 321 ADKSQWSNKK--TFVLPISQMNFVHGDR---LVVGASRDSG-KVLMRKANLLRNQHARFF 367

BLAST of MS018738 vs. TAIR 10
Match: AT2G16810.1 (F-box and associated interaction domains-containing protein )

HSP 1 Score: 73.6 bits (179), Expect = 4.2e-13
Identity = 74/256 (28.91%), Postives = 119/256 (46.48%), Query Frame = 0

Query: 17  LPEDIIIFHILTKLPTKSLLRFRSVSKSWNSATSHPLFLSLRHLRPRFLSHVLISASQPN 76
           +P+D++I  ILT+LP KS++RF+ VSK W+S       LS R+   RF    LI  SQP 
Sbjct: 41  IPQDLLI-EILTRLPPKSVMRFKCVSKFWSS------LLSSRYFCNRF----LIVPSQPQ 100

Query: 77  SSCHHLL-------SAALGPLSPLTHLLSLPLRHSLAVKPANGLLLLHFPNDLYSDRPDL 136
            S +  L        + +   +P T   S      L ++   G  L      ++  R +L
Sbjct: 101 PSLYMCLLDRYNYSKSLILSSAPSTSPYSFVFDQDLTIRKMGGFFLRILRGFIFFTR-NL 160

Query: 137 PAYIFNPRARDIIPLP----PNAPTNQKVKILYHLGYDALGDEYKVLQIWRFI-DTNLEM 196
            A I+NP  R ++ LP     +        ILY + +D + D YK+L    +  D +L+ 
Sbjct: 161 KARIYNPTTRQLVILPTIKESDIIAGPPYNILYFICHDPVNDRYKLLCTVSYASDNDLQN 220

Query: 197 SESGMVFKVLSLGSGKWRRIRPRRIPFFCSSYCALFSPCCASNGGIHWV--VHPKKFVLV 256
            +S +   VL  G G W+R+     P    S+  L       NG ++++    P   +LV
Sbjct: 221 LKSELWIFVLEAG-GSWKRV-ANEFPHHVPSHLDL-----NMNGVLYFLAWTDPHTCMLV 277

Query: 257 -FNLHDEEFASIPFPQ 258
            F++  EEF ++  P+
Sbjct: 281 SFDVRSEEFNTMQVPR 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146376.19.3e-16483.38F-box/kelch-repeat protein At4g19930-like [Momordica charantia][more]
XP_008385265.29.0e-4232.13F-box protein At4g19940-like [Malus domestica] >XP_017191226.2 F-box protein At4... [more]
TQD90884.12.6e-4132.13hypothetical protein C1H46_023563 [Malus baccata][more]
XP_009346574.12.6e-4132.77PREDICTED: putative F-box protein At5g62660 [Pyrus x bretschneideri] >KAB2606747... [more]
RXH83739.15.8e-4132.10hypothetical protein DVH24_005992 [Malus domestica][more]
Match NameE-valueIdentityDescription
O494211.3e-1927.11F-box protein At4g19940 OS=Arabidopsis thaliana OX=3702 GN=At4g19940 PE=4 SV=1[more]
O494201.1e-1826.67F-box/kelch-repeat protein At4g19930 OS=Arabidopsis thaliana OX=3702 GN=At4g1993... [more]
Q9FJJ41.1e-1326.03F-box protein At5g62510 OS=Arabidopsis thaliana OX=3702 GN=At5g62510 PE=2 SV=1[more]
Q9FT502.0e-1226.06Putative F-box protein At3g52320 OS=Arabidopsis thaliana OX=3702 GN=At3g52320 PE... [more]
Q84X435.9e-1228.91F-box only protein 8 OS=Arabidopsis thaliana OX=3702 GN=FBX8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CZ644.5e-16483.38F-box/kelch-repeat protein At4g19930-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A2P2K4Y21.4e-4534.01F-box protein At5g62510-like OS=Rhizophora mucronata OX=61149 PE=4 SV=1[more]
A0A5N5FV351.3e-4132.77F-box protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_006464 P... [more]
A0A540LWM91.3e-4132.13F-box domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_023563 PE=4 ... [more]
A0A498IKL32.8e-4132.10tRNA-intron lyase OS=Malus domestica OX=3750 GN=DVH24_005992 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19940.19.3e-2127.11F-box and associated interaction domains-containing protein [more]
AT4G19930.17.9e-2026.67F-box and associated interaction domains-containing protein [more]
AT5G62510.17.6e-1526.03F-box family protein [more]
AT3G52320.11.4e-1326.06F-box and associated interaction domains-containing protein [more]
AT2G16810.14.2e-1328.91F-box and associated interaction domains-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 17..58
e-value: 2.5E-5
score: 33.7
IPR001810F-box domainPFAMPF00646F-boxcoord: 14..52
e-value: 2.6E-9
score: 36.7
IPR013187F-box associated domain, type 3PFAMPF08268FBA_3coord: 237..315
e-value: 1.7E-6
score: 28.0
NoneNo IPR availableGENE3D1.20.1280.50coord: 5..54
e-value: 9.1E-7
score: 30.5
NoneNo IPR availablePANTHERPTHR31111BNAA05G37150D PROTEIN-RELATEDcoord: 15..385
NoneNo IPR availablePANTHERPTHR31111:SF22BNAA05G37150D PROTEINcoord: 15..385
IPR017451F-box associated interaction domainTIGRFAMTIGR01640TIGR01640coord: 131..360
e-value: 9.7E-24
score: 81.9
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 14..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018738.1MS018738.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding