MS018692 (gene) Bitter gourd (TR) v1

Overview
NameMS018692
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhosphoribosyl-AMP cyclohydrolase
Locationscaffold313: 1107943 .. 1108967 (+)
RNA-Seq ExpressionMS018692
SyntenyMS018692
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCAGGTTGAGACGCTGTTAGACAGCATAAAATGGGATGACAAAGGTCTGGCAGTAGCTATAGCTCAAAATGTTGATACAGGAGCAATATTAATGCAAGGATTTGCGAATAGGGAGGCTGTTGCTACAACTGCTTCCCAACGAAAGGCTACATTCTACAGTCGATCGCGATCACAGCTGTGGACAAAGGGAGAGACCTCTAATAACTTCATAAACATTCACGACATATTCATTGATTGTGATCGTGATTCTGTATGGCTCTAACCGAAAGCGACCTAAACAATCACATCAGCTTATTGTGGTTTACTGTGCATAGCTAACTCAGTTTTAAAACTGATTGTTAGATCATTTATCTCGGGAAGCCTGACGGGCCTACTTGTCATACTGGAGCCGAGACATGCTATTATACATCACTAGATGACTTGATAGAGAGTCAACAGGTCTGCTGCTTTAACAGATTTGTTTATGGATAACTAAGAGAATGAGATTGTTTTGACCTATACTTGTGTATTAATTCTTGTAGGAGGTGAACCATAAGTTGGCCTTGTCTACTTTGTACTCATTAGAGTCCACAATCTCCGAACGCAAAGTGGAAATAGCTGTTCCACAAAATGGTAAACCCTCGTGGACGAGACGACTGTTGCTTGACAACAACTTGCTCTGCTCTAAAATCAGGTATCGTATTTTAAATTGACATTTTGTTTACCAGACTACATTTGAAATTTTAATTGTGGCACCATAACATTCTCTAGTAAATCACTTTCTGTCCCATGTTCTAGTCCCTAGCATTTGACAGCTTCAGTACTTGTCCTGGTTCTGATAGGGAAGAAGCGGACGAGTTGTGTCGAACGCTGGAAGAGAATGAGGATAAGTCTCGCACTGCCTCGGAAATGGCAGATGTGCTGTATCATGCCATGGTTCTCCTGGCAGTCAAAGAGGTAGAGATGGAGGATGTTCTTCAAATTCTGCGTCAGAGATTTTCTCAGTCGGGTATCGAGGAGAAGAGGAATCGACCGCCCAAA

mRNA sequence

ATGTTTCAGGTTGAGACGCTGTTAGACAGCATAAAATGGGATGACAAAGGTCTGGCAGTAGCTATAGCTCAAAATGTTGATACAGGAGCAATATTAATGCAAGGATTTGCGAATAGGGAGGCTGTTGCTACAACTGCTTCCCAACGAAAGGCTACATTCTACAGTCGATCGCGATCACAGCTGTGGACAAAGGGAGAGACCTCTAATAACTTCATAAACATTCACGACATATTCATTGATTGTGATCGTGATTCTATCATTTATCTCGGGAAGCCTGACGGGCCTACTTGTCATACTGGAGCCGAGACATGCTATTATACATCACTAGATGACTTGATAGAGAGTCAACAGGAGGTGAACCATAAGTTGGCCTTGTCTACTTTGTACTCATTAGAGTCCACAATCTCCGAACGCAAAGTGGAAATAGCTGTTCCACAAAATGGTAAACCCTCGTGGACGAGACGACTGTTGCTTGACAACAACTTGCTCTGCTCTAAAATCAGGGAAGAAGCGGACGAGTTGTGTCGAACGCTGGAAGAGAATGAGGATAAGTCTCGCACTGCCTCGGAAATGGCAGATGTGCTGTATCATGCCATGGTTCTCCTGGCAGTCAAAGAGGTAGAGATGGAGGATGTTCTTCAAATTCTGCGTCAGAGATTTTCTCAGTCGGGTATCGAGGAGAAGAGGAATCGACCGCCCAAA

Coding sequence (CDS)

ATGTTTCAGGTTGAGACGCTGTTAGACAGCATAAAATGGGATGACAAAGGTCTGGCAGTAGCTATAGCTCAAAATGTTGATACAGGAGCAATATTAATGCAAGGATTTGCGAATAGGGAGGCTGTTGCTACAACTGCTTCCCAACGAAAGGCTACATTCTACAGTCGATCGCGATCACAGCTGTGGACAAAGGGAGAGACCTCTAATAACTTCATAAACATTCACGACATATTCATTGATTGTGATCGTGATTCTATCATTTATCTCGGGAAGCCTGACGGGCCTACTTGTCATACTGGAGCCGAGACATGCTATTATACATCACTAGATGACTTGATAGAGAGTCAACAGGAGGTGAACCATAAGTTGGCCTTGTCTACTTTGTACTCATTAGAGTCCACAATCTCCGAACGCAAAGTGGAAATAGCTGTTCCACAAAATGGTAAACCCTCGTGGACGAGACGACTGTTGCTTGACAACAACTTGCTCTGCTCTAAAATCAGGGAAGAAGCGGACGAGTTGTGTCGAACGCTGGAAGAGAATGAGGATAAGTCTCGCACTGCCTCGGAAATGGCAGATGTGCTGTATCATGCCATGGTTCTCCTGGCAGTCAAAGAGGTAGAGATGGAGGATGTTCTTCAAATTCTGCGTCAGAGATTTTCTCAGTCGGGTATCGAGGAGAAGAGGAATCGACCGCCCAAA

Protein sequence

MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK
Homology
[Close]
BLAST of MS018692 vs. NCBI nr
Match: XP_022141529.1 (histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 450.3 bits (1157), Expect = 1.0e-122
Identity = 228/232 (98.28%), Postives = 231/232 (99.57%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +VE LLDS+KWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW
Sbjct: 12  KVEMLLDSMKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 71

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDL+ESQQEVNHK
Sbjct: 72  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLMESQQEVNHK 131

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE
Sbjct: 132 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 191

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK
Sbjct: 192 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 243

[Close]
BLAST of MS018692 vs. NCBI nr
Match: XP_022141528.1 (histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 450.3 bits (1157), Expect = 1.0e-122
Identity = 228/232 (98.28%), Postives = 231/232 (99.57%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +VE LLDS+KWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW
Sbjct: 51  KVEMLLDSMKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 110

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDL+ESQQEVNHK
Sbjct: 111 TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLMESQQEVNHK 170

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE
Sbjct: 171 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 230

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK
Sbjct: 231 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 282

[Close]
BLAST of MS018692 vs. NCBI nr
Match: XP_023546566.1 (histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.5 bits (1072), Expect = 7.4e-113
Identity = 213/234 (91.03%), Postives = 221/234 (94.44%), Query Frame = 0 

Query: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQ 60
           MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQ
Sbjct: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQ 60

Query: 61  LWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVN 120
           LWTKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+  
Sbjct: 61  LWTKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAK 120

Query: 121 HKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEE 180
            KLALSTLYSLESTISERK EI VPQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEE
Sbjct: 121 DKLALSTLYSLESTISERKAEIEVPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEE 180

Query: 181 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           NEDKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 181 NEDKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 233

[Close]
BLAST of MS018692 vs. NCBI nr
Match: XP_022990694.1 (histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 416.0 bits (1068), Expect = 2.1e-112
Identity = 212/234 (90.60%), Postives = 220/234 (94.02%), Query Frame = 0 

Query: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQ 60
           MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQ
Sbjct: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQ 60

Query: 61  LWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVN 120
           LWTKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+  
Sbjct: 61  LWTKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAK 120

Query: 121 HKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEE 180
            KLALSTLYSLESTISERK EI  PQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEE
Sbjct: 121 DKLALSTLYSLESTISERKAEIEAPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEE 180

Query: 181 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           NEDKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 181 NEDKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 233

[Close]
BLAST of MS018692 vs. NCBI nr
Match: XP_022964073.1 (histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 414.8 bits (1065), Expect = 4.8e-112
Identity = 211/234 (90.17%), Postives = 220/234 (94.02%), Query Frame = 0 

Query: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQ 60
           MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQ
Sbjct: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQ 60

Query: 61  LWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVN 120
           LWTKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+  
Sbjct: 61  LWTKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAK 120

Query: 121 HKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEE 180
            KLALSTLY+LESTISERK EI  PQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEE
Sbjct: 121 DKLALSTLYTLESTISERKAEIEAPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEE 180

Query: 181 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           NEDKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 181 NEDKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 233

[Close]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match: O82768 (Histidine biosynthesis bifunctional protein hisIE, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HISN2 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 3.5e-97
Identity = 176/229 (76.86%), Postives = 202/229 (88.21%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +V+ LLD IKWDDKGLAVAIAQNVDTGA+LMQGF NREA++TT S RKATF+SRSRS LW
Sbjct: 51  KVDNLLDRIKWDDKGLAVAIAQNVDTGAVLMQGFVNREALSTTISSRKATFFSRSRSTLW 110

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINI D+++DCDRDSIIYLG PDGPTCHTG ETCYYTS+ D + + +   +K
Sbjct: 111 TKGETSNNFINILDVYVDCDRDSIIYLGTPDGPTCHTGEETCYYTSVFDQLNNDEASGNK 170

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LAL+TLYSLES IS+RK E  VPQ GKPSWTRRLL D+ LLCSKIREEADELCRTLE+NE
Sbjct: 171 LALTTLYSLESIISKRKEESTVPQEGKPSWTRRLLTDDALLCSKIREEADELCRTLEDNE 230

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNR 232
           + SRT SEMADVLYHAMVLL+ + V+MEDVL++LR+RFSQSGIEEK+NR
Sbjct: 231 EVSRTPSEMADVLYHAMVLLSKRGVKMEDVLEVLRKRFSQSGIEEKQNR 279

[Close]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match: Q7MAQ8 (Histidine biosynthesis bifunctional protein HisIE OS=Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) OX=273121 GN=hisI PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-28
Identity = 81/231 (35.06%), Postives = 128/231 (55.41%), Query Frame = 0 

Query: 4   VETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLWT 63
           + TLLD I W+   L  AI Q   +G +LM  + N+EA+  T S + A ++SRS+ ++W 
Sbjct: 1   MNTLLDGIDWEKHPLLPAIVQERGSGEVLMLAYMNQEALNLTLSTQVAHYFSRSKGRIWK 60

Query: 64  KGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHKL 123
           KGE+S +   IH+IF+DCD D+I+   +  G  CHTG ++C++  + +L  S    +   
Sbjct: 61  KGESSGHIQKIHEIFLDCDSDTILLQVEQVGVACHTGRKSCFFQKV-ELDSSLSLTSEAP 120

Query: 124 ALSTLYSLESTISERKVEIAVPQNGKP--SWTRRLL-LDNNLLCSKIREEADELCRTLEE 183
             S LY +   +     E+   Q   P  S+T +L     N +  KI EEA EL   +++
Sbjct: 121 DTSALYGVVDRLYH---ELLARQGADPQSSYTAKLFSKGENTIGKKIVEEAAELSFAIKD 180

Query: 184 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNR 232
           + + S    E AD+LYHA+V LA + +  + + Q L++R   SGI EK +R
Sbjct: 181 SSE-SEIVYEAADLLYHALVGLAFRGIHPDKIKQELQRRQGVSGIAEKNSR 226

[Close]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match: Q9RWD6 (Histidine biosynthesis bifunctional protein HisIE OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=hisI PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.9e-27
Identity = 78/224 (34.82%), Postives = 123/224 (54.91%), Query Frame = 0 

Query: 6   TLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLWTKG 65
           T L  + +D  GL   + Q+  +GA+LMQ +A+R AV  T   R+AT+YSRSR + W KG
Sbjct: 2   TDLSELNFDPSGLIPVVTQDARSGAVLMQAYADRAAVERTLDTREATYYSRSRGEQWVKG 61

Query: 66  ETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSL--DDLIESQQEVNHKL 125
           +TS +   +  + +DCD DS++Y  +  GP CHTG  +C+Y  L  DD  ++  +     
Sbjct: 62  QTSGHTQRVVSVHVDCDGDSLLYRVEQTGPACHTGEYSCFYRPLLEDDAPDTGLD----- 121

Query: 126 ALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDN-NLLCSKIREEADELCRTLEENE 185
              TL  + +TI+ER   +      + S+  RL     + +  KI EEA E+     +N 
Sbjct: 122 --GTLERVYATITERLATLP-----EGSYVARLHAGGLDRVLKKISEEAGEVL-LAAKNA 181

Query: 186 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIE 227
           D++  A+E AD+L+H +  LA   V   DV  +L+ R  +SG++
Sbjct: 182 DRAELATETADLLFHTLFALAEVGVSPADVAAVLQGREGKSGLK 212

[Close]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match: Q9CLL8 (Histidine biosynthesis bifunctional protein HisIE OS=Pasteurella multocida (strain Pm70) OX=272843 GN=hisI PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.1e-26
Identity = 80/216 (37.04%), Postives = 123/216 (56.94%), Query Frame = 0 

Query: 11  IKWDD-KGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLWTKGETSN 70
           I W     L   I QNV T  +LM G+ N+EA+  T ++++ TF+SR++++LWTKGETS 
Sbjct: 3   INWQKVDNLLPVIIQNVATCEVLMLGYMNQEALEKTLAEKRVTFFSRTKNRLWTKGETSG 62

Query: 71  NFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHKLALSTLY 130
           +F+N+ D+ +DCD D+++ L  P G TCHTGAE+C+Y         Q E   +     L 
Sbjct: 63  HFLNVVDMSLDCDNDTLLILVNPIGETCHTGAESCFY---------QFEKTTQPDWIFLS 122

Query: 131 SLESTISERKVEIAVPQNGKPSWTRRLLL-DNNLLCSKIREEADE--LCRTLEENEDKSR 190
            LE  I+ RK   A P++   S+T +L       +  K+ EE  E  L  T+   +DK+ 
Sbjct: 123 KLERLIASRKG--ADPES---SYTAQLYAKGTKRIAQKVGEEGVETALAATV---KDKAE 182

Query: 191 TASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQ 223
           T  E AD++YH  VLL   ++   DV+  L++R ++
Sbjct: 183 TICEAADLVYHLTVLLQDADLSWSDVIHKLKERHTK 201

[Close]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match: Q8ZFY1 (Histidine biosynthesis bifunctional protein HisIE OS=Yersinia pestis OX=632 GN=hisI PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-26
Identity = 72/217 (33.18%), Postives = 117/217 (53.92%), Query Frame = 0 

Query: 5   ETLLDSIKWDD-KGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLWT 64
           E  ++ + W+    L  AI Q+  +G +LM G+ N+EA+A T    K TF+SR++ +LWT
Sbjct: 4   EQQINQLDWEKVDHLMPAIVQHAVSGEVLMMGYMNQEALAVTEKTGKVTFFSRTKQRLWT 63

Query: 65  KGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHKL 124
           KGE+S +F+N+ +I+ DCD D+++ L KP GPTCH G  +C+  +  D            
Sbjct: 64  KGESSGHFLNVVNIYPDCDNDTLLILVKPIGPTCHLGNSSCFAPAASD------------ 123

Query: 125 ALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLD-NNLLCSKIREEADELCRTLEENE 184
             S LY LE  ++ RK       +   S+T +L       +  K+ EE  E       N 
Sbjct: 124 -WSFLYQLEQLLASRK-----SADPASSYTAKLYASGTKRIAQKVGEEGVETALAATVN- 183

Query: 185 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQR 220
           D+    +E +D++YH +VLL  ++++   V+  LR+R
Sbjct: 184 DREELTNEASDLMYHLLVLLQDQDLDFSTVIGRLRER 201

[Close]
BLAST of MS018692 vs. ExPASy TrEMBL
Match: A0A6J1CKR4 (Phosphoribosyl-AMP cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111011879 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.0e-123
Identity = 228/232 (98.28%), Postives = 231/232 (99.57%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +VE LLDS+KWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW
Sbjct: 51  KVEMLLDSMKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 110

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDL+ESQQEVNHK
Sbjct: 111 TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLMESQQEVNHK 170

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE
Sbjct: 171 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 230

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK
Sbjct: 231 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 282

[Close]
BLAST of MS018692 vs. ExPASy TrEMBL
Match: A0A6J1CIX5 (Phosphoribosyl-AMP cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111011879 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.0e-123
Identity = 228/232 (98.28%), Postives = 231/232 (99.57%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +VE LLDS+KWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW
Sbjct: 12  KVEMLLDSMKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 71

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDL+ESQQEVNHK
Sbjct: 72  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLMESQQEVNHK 131

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE
Sbjct: 132 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 191

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK
Sbjct: 192 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 243

[Close]
BLAST of MS018692 vs. ExPASy TrEMBL
Match: A0A6J1JSQ7 (Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487506 PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 1.0e-112
Identity = 212/234 (90.60%), Postives = 220/234 (94.02%), Query Frame = 0 

Query: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQ 60
           MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQ
Sbjct: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQ 60

Query: 61  LWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVN 120
           LWTKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+  
Sbjct: 61  LWTKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAK 120

Query: 121 HKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEE 180
            KLALSTLYSLESTISERK EI  PQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEE
Sbjct: 121 DKLALSTLYSLESTISERKAEIEAPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEE 180

Query: 181 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           NEDKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 181 NEDKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 233

[Close]
BLAST of MS018692 vs. ExPASy TrEMBL
Match: A0A6J1HJR6 (Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111464194 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.3e-112
Identity = 211/234 (90.17%), Postives = 220/234 (94.02%), Query Frame = 0 

Query: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQ 60
           MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQ
Sbjct: 1   MFQVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQ 60

Query: 61  LWTKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVN 120
           LWTKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+  
Sbjct: 61  LWTKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAK 120

Query: 121 HKLALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEE 180
            KLALSTLY+LESTISERK EI  PQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEE
Sbjct: 121 DKLALSTLYTLESTISERKAEIEAPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEE 180

Query: 181 NEDKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           NEDKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 181 NEDKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 233

[Close]
BLAST of MS018692 vs. ExPASy TrEMBL
Match: A0A6J1JU12 (Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487506 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.7e-111
Identity = 209/232 (90.09%), Postives = 218/232 (93.97%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +VETLLDSIKWDDKGLAVAIAQNVDTGAILMQGF N+EA+ATT SQRKATFYSRSRSQLW
Sbjct: 51  KVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFVNKEAIATTVSQRKATFYSRSRSQLW 110

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINIHDIF+DCDRDSIIYLGKP GPTCHTGAETCYYTSLDD I +QQ+   K
Sbjct: 111 TKGETSNNFINIHDIFLDCDRDSIIYLGKPVGPTCHTGAETCYYTSLDDSI-NQQDAKDK 170

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LALSTLYSLESTISERK EI  PQNGKPSWT+RLLLDNNLLCSKIREEADELCRTLEENE
Sbjct: 171 LALSTLYSLESTISERKAEIEAPQNGKPSWTKRLLLDNNLLCSKIREEADELCRTLEENE 230

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNRPPK 235
           DKSR ASEMADVLYHAMVLLAVK VEMEDVLQILRQRFSQSGIEEK++R PK
Sbjct: 231 DKSRAASEMADVLYHAMVLLAVKGVEMEDVLQILRQRFSQSGIEEKKSRVPK 281

[Close]
BLAST of MS018692 vs. TAIR 10
Match: AT1G31860.1 (histidine biosynthesis bifunctional protein (HISIE) )

HSP 1 Score: 355.9 bits (912), Expect = 2.5e-98
Identity = 176/229 (76.86%), Postives = 202/229 (88.21%), Query Frame = 0 

Query: 3   QVETLLDSIKWDDKGLAVAIAQNVDTGAILMQGFANREAVATTASQRKATFYSRSRSQLW 62
           +V+ LLD IKWDDKGLAVAIAQNVDTGA+LMQGF NREA++TT S RKATF+SRSRS LW
Sbjct: 51  KVDNLLDRIKWDDKGLAVAIAQNVDTGAVLMQGFVNREALSTTISSRKATFFSRSRSTLW 110

Query: 63  TKGETSNNFINIHDIFIDCDRDSIIYLGKPDGPTCHTGAETCYYTSLDDLIESQQEVNHK 122
           TKGETSNNFINI D+++DCDRDSIIYLG PDGPTCHTG ETCYYTS+ D + + +   +K
Sbjct: 111 TKGETSNNFINILDVYVDCDRDSIIYLGTPDGPTCHTGEETCYYTSVFDQLNNDEASGNK 170

Query: 123 LALSTLYSLESTISERKVEIAVPQNGKPSWTRRLLLDNNLLCSKIREEADELCRTLEENE 182
           LAL+TLYSLES IS+RK E  VPQ GKPSWTRRLL D+ LLCSKIREEADELCRTLE+NE
Sbjct: 171 LALTTLYSLESIISKRKEESTVPQEGKPSWTRRLLTDDALLCSKIREEADELCRTLEDNE 230

Query: 183 DKSRTASEMADVLYHAMVLLAVKEVEMEDVLQILRQRFSQSGIEEKRNR 232
           + SRT SEMADVLYHAMVLL+ + V+MEDVL++LR+RFSQSGIEEK+NR
Sbjct: 231 EVSRTPSEMADVLYHAMVLLSKRGVKMEDVLEVLRKRFSQSGIEEKQNR 279

The following BLAST results are available for this feature:
BLAST of MS018692 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022141529.11.0e-12298.28histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Mom... [more]
XP_022141528.11.0e-12298.28histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X1 [Mom... [more]
XP_023546566.17.4e-11391.03histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cuc... [more]
XP_022990694.12.1e-11290.60histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cuc... [more]
XP_022964073.14.8e-11290.17histidine biosynthesis bifunctional protein hisIE, chloroplastic isoform X2 [Cuc... [more]
BLAST of MS018692 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O827683.5e-9776.86Histidine biosynthesis bifunctional protein hisIE, chloroplastic OS=Arabidopsis ... [more]
Q7MAQ84.5e-2835.06Histidine biosynthesis bifunctional protein HisIE OS=Wolinella succinogenes (str... [more]
Q9RWD62.9e-2734.82Histidine biosynthesis bifunctional protein HisIE OS=Deinococcus radiodurans (st... [more]
Q9CLL81.1e-2637.04Histidine biosynthesis bifunctional protein HisIE OS=Pasteurella multocida (stra... [more]
Q8ZFY12.4e-2633.18Histidine biosynthesis bifunctional protein HisIE OS=Yersinia pestis OX=632 GN=h... [more]
BLAST of MS018692 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CKR45.0e-12398.28Phosphoribosyl-AMP cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111011879... [more]
A0A6J1CIX55.0e-12398.28Phosphoribosyl-AMP cyclohydrolase OS=Momordica charantia OX=3673 GN=LOC111011879... [more]
A0A6J1JSQ71.0e-11290.60Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487506 PE... [more]
A0A6J1HJR62.3e-11290.17Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111464194 ... [more]
A0A6J1JU125.7e-11190.09Phosphoribosyl-AMP cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111487506 PE... [more]
BLAST of MS018692 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G31860.12.5e-9876.86histidine biosynthesis bifunctional protein (HISIE) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 164..184
NoneNo IPR availableGENE3D1.10.287.1080coord: 124..221
e-value: 1.9E-23
score: 84.6
NoneNo IPR availablePANTHERPTHR42945HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEINcoord: 3..231
NoneNo IPR availablePANTHERPTHR42945:SF7BNAC05G24080D PROTEINcoord: 3..231
NoneNo IPR availableSUPERFAMILY101386all-alpha NTP pyrophosphatasescoord: 127..220
IPR038019Phosphoribosyl-AMP cyclohydrolase domain superfamilyGENE3D3.10.20.810coord: 3..97
e-value: 1.1E-33
score: 116.8
IPR038019Phosphoribosyl-AMP cyclohydrolase domain superfamilySUPERFAMILY141734HisI-likecoord: 7..111
IPR008179Phosphoribosyl-ATP pyrophosphohydrolaseTIGRFAMTIGR03188TIGR03188coord: 128..216
e-value: 1.3E-19
score: 68.2
IPR008179Phosphoribosyl-ATP pyrophosphohydrolaseCDDcd11534NTP-PPase_HisIE_likecoord: 128..216
e-value: 1.15991E-18
score: 75.5728
IPR021130Phosphoribosyl-ATP pyrophosphohydrolase-likePFAMPF01503PRA-PHcoord: 145..219
e-value: 1.9E-11
score: 44.3
IPR002496Phosphoribosyl-AMP cyclohydrolase domainPFAMPF01502PRA-CHcoord: 33..106
e-value: 8.7E-28
score: 96.0
IPR023019Histidine biosynthesis bifunctional protein HisIEHAMAPMF_01019HisIEcoord: 9..221
score: 29.935205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018692.1MS018692.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000105 histidine biosynthetic process
molecular_function GO:0004635 phosphoribosyl-AMP cyclohydrolase activity
molecular_function GO:0004636 phosphoribosyl-ATP diphosphatase activity