MS018689 (gene) Bitter gourd (TR) v1

BLAST of MS018689 vs. NCBI nr
Match: XP_022141422.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1613.2 bits (4176), Expect = 0.0e+00
Identity = 821/824 (99.64%), Postives = 823/824 (99.88%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG
Sbjct: 1   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 60

Query: 62  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 121
           TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ
Sbjct: 61  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 120

Query: 122 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181
           KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE
Sbjct: 121 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 180

Query: 182 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 241
           YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKG+VAIQKMVVDLM
Sbjct: 181 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGKVAIQKMVVDLM 240

Query: 242 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 301
           ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD
Sbjct: 241 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 300

Query: 302 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 361
           VGFGKTEVALRAIF VVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 301 VGFGKTEVALRAIFCVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 360

Query: 362 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 421
           KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 361 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 420

Query: 422 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 481
           IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 421 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 480

Query: 482 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 541
           GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 481 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 540

Query: 542 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 601
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE
Sbjct: 541 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 600

Query: 602 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 661
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 601 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 660

Query: 662 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 721
           HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ
Sbjct: 661 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 720

Query: 722 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 781
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMN+KVFKLITDSMTSEVHQ
Sbjct: 721 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNRKVFKLITDSMTSEVHQ 780

Query: 782 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY
Sbjct: 781 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 824

BLAST of MS018689 vs. NCBI nr
Match: XP_038907244.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 769/825 (93.21%), Postives = 799/825 (96.85%), Query Frame = 0

Query: 2   MALL-SPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVP 61
           MALL SPAPDVS+ RPLVAF+LGSFP    LFNRN T R  PKR +S+TNV+YAEDV+VP
Sbjct: 1   MALLFSPAPDVSSSRPLVAFKLGSFPSLRGLFNRNSTFRHFPKRYVSITNVIYAEDVIVP 60

Query: 62  GTAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGL 121
           G AKSARRREQIELERDSISILNER+RR+HGKRESSRT MD+EEADRYIQMVKEQQQRGL
Sbjct: 61  GIAKSARRREQIELERDSISILNERIRRFHGKRESSRTVMDSEEADRYIQMVKEQQQRGL 120

Query: 122 QKLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFI 181
           QKLKGDRQR +SDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFI
Sbjct: 121 QKLKGDRQRKDSDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFI 180

Query: 182 EYADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDL 241
           EYADGMAKLPVKQASRMLYRYNLPNE KRPRTLSKLNDT+TWEKRKTKG+VAIQKMVVDL
Sbjct: 181 EYADGMAKLPVKQASRMLYRYNLPNENKRPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDL 240

Query: 242 MELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICG 301
           MELYLHRLKQRR PYPKCSAME+F+AQFPYEPTVDQKEAF DVE+DLT+RETPMDRLICG
Sbjct: 241 MELYLHRLKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTQRETPMDRLICG 300

Query: 302 DVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQ 361
           DVGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFS FPDVQIGLLSRFQ
Sbjct: 301 DVGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSLFPDVQIGLLSRFQ 360

Query: 362 TKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKT 421
           TKAEKEKHL+MIKEGQL+IIVGTHSLLGDRVVY+ LGLLVVDEEQRFGVKQKEKIASFKT
Sbjct: 361 TKAEKEKHLEMIKEGQLNIIVGTHSLLGDRVVYSKLGLLVVDEEQRFGVKQKEKIASFKT 420

Query: 422 SIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELE 481
           SIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAI+YELE
Sbjct: 421 SIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIRYELE 480

Query: 482 RGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC 541
           RGGQVFYVLPRIKGLEEVK+FLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC
Sbjct: 481 RGGQVFYVLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC 540

Query: 542 TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL 601
           TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLS++AL
Sbjct: 541 TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSEEAL 600

Query: 602 ERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVD 661
           ERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKVD
Sbjct: 601 ERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVD 660

Query: 662 EHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRR 721
           EHRVVSVPYQSVKID+DI PHLPSEYINYLENPMKI+NGAE AAETDIW LMQFTENLRR
Sbjct: 661 EHRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKILNGAESAAETDIWTLMQFTENLRR 720

Query: 722 QHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVH 781
           QHGKEPYSMEILLKKLYVRRMAADLGISRIYASGK VCMET MNKKVFKLI+DSMTSEVH
Sbjct: 721 QHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKAVCMETNMNKKVFKLISDSMTSEVH 780

Query: 782 QNCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           +NCLSFEEH IKAGLLLELP+EQLLNWIFECLVELHASLPALIKY
Sbjct: 781 RNCLSFEEHQIKAGLLLELPREQLLNWIFECLVELHASLPALIKY 825

BLAST of MS018689 vs. NCBI nr
Match: XP_023529599.1 (ATP-dependent DNA helicase At3g02060, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1508.0 bits (3903), Expect = 0.0e+00
Identity = 761/824 (92.35%), Postives = 796/824 (96.60%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           MALL P   VS+ RPLVAFRLGSFP+ W LFNRNC LR C KRC+S+TNVVYAEDVMVPG
Sbjct: 1   MALLLPTAVVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHCSKRCVSITNVVYAEDVMVPG 60

Query: 62  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 121
            AKSARRREQIELE DSI++LNER+RR+H KRESS+TAM+AEEADRYIQMVKEQQQRGLQ
Sbjct: 61  AAKSARRREQIELEHDSITVLNERIRRFHVKRESSKTAMEAEEADRYIQMVKEQQQRGLQ 120

Query: 122 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181
           KLKG+RQ+ ESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVFIE
Sbjct: 121 KLKGNRQKKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIE 180

Query: 182 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 241
           YADGMAKL VKQASRMLYRY+LPNE KRPRTLSKLNDT+TWEKRKTKG++AIQKMVVDLM
Sbjct: 181 YADGMAKLSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLM 240

Query: 242 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 301
           ELYLHRLKQRRPPYPKCSAME+FAAQF Y PTVDQKEAF DVEKDLTERETPMDRLICGD
Sbjct: 241 ELYLHRLKQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGD 300

Query: 302 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 361
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 301 VGFGKTEVALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 360

Query: 362 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 421
           K+EK+KHL+MIKEGQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 361 KSEKDKHLEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 420

Query: 422 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 481
           IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKVKSAIKYELER
Sbjct: 421 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSAFSKEKVKSAIKYELER 480

Query: 482 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 541
           GGQVFYV PRIKGLEEVKDFLEASFPDIEI LAHGKQYSKQLEETMENFALG IKILICT
Sbjct: 481 GGQVFYVFPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFALGAIKILICT 540

Query: 542 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 601
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALE
Sbjct: 541 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALE 600

Query: 602 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 661
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 601 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDE 660

Query: 662 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 721
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ
Sbjct: 661 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 720

Query: 722 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 781
           HGKEPYSMEILLKKLYVRRMAADLGI RIYASGKTVCMETKMNKKVFKLI++SM SEVH+
Sbjct: 721 HGKEPYSMEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHR 780

Query: 782 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCL+FEEH IKAGLLLELP+EQLLNWIFECLVELHASLPALIKY
Sbjct: 781 NCLTFEEHQIKAGLLLELPREQLLNWIFECLVELHASLPALIKY 824

BLAST of MS018689 vs. NCBI nr
Match: XP_008465367.1 (PREDICTED: ATP-dependent DNA helicase At3g02060, chloroplastic [Cucumis melo])

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 759/824 (92.11%), Postives = 796/824 (96.60%), Query Frame = 0

Query: 4   LLSPAPDVSTFRPLVAFRLGSFPR--QWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 63
           L SPAPDVS+ RPL+AF+L SFP    W LFNRNCT R  P RC+SVTNV YAEDV+VPG
Sbjct: 4   LFSPAPDVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPG 63

Query: 64  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 123
           TAKSARRR+QIELERDSIS+LNER+ R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQ
Sbjct: 64  TAKSARRRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQ 123

Query: 124 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 183
           KLKGD+QR ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVFIE
Sbjct: 124 KLKGDKQRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIE 183

Query: 184 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 243
           YADGMAKLPVKQASRMLYRYNLPNE ++PRTLSKLNDT+TWEKRKTKG+VAIQKMVVDLM
Sbjct: 184 YADGMAKLPVKQASRMLYRYNLPNENRKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLM 243

Query: 244 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 303
           ELYLHRLKQRR PYPKCSAME+F+AQFPYEPTVDQKEAF DVE+DLTERETPMDRLICGD
Sbjct: 244 ELYLHRLKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGD 303

Query: 304 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 304 VGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363

Query: 364 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 423
           KAEKEKHL+MIKEGQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 364 KAEKEKHLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 423

Query: 424 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483
           +DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 424 VDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483

Query: 484 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543
           GGQ+FYVLPRIKGLEEVK+FLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 484 GGQIFYVLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543

Query: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 603
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALE
Sbjct: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALE 603

Query: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663
           RLAALE+CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 604 RLAALEDCRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663

Query: 664 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 723
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKI+NGAE+AAETDIW LMQFTENLRR 
Sbjct: 664 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRH 723

Query: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 783
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGK VCMET MNKKVF+LI+DSMTSEVH+
Sbjct: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHR 783

Query: 784 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCLSFEEH IKAGLLLELP+EQLLNW+FECLVELHASLPALIKY
Sbjct: 784 NCLSFEEHQIKAGLLLELPREQLLNWVFECLVELHASLPALIKY 827

BLAST of MS018689 vs. NCBI nr
Match: XP_004150202.1 (ATP-dependent DNA helicase At3g02060, chloroplastic [Cucumis sativus])

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 763/824 (92.60%), Postives = 796/824 (96.60%), Query Frame = 0

Query: 4   LLSPAPDVSTFRPLVAFRLGSFP--RQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 63
           LLSPAPDVS+  P++ F+L SFP  R W LFNRN T R  P RC+S+TNVVYAEDV+VPG
Sbjct: 4   LLSPAPDVSSSPPVLPFKLCSFPTLRGWGLFNRNSTFRHFPNRCVSITNVVYAEDVIVPG 63

Query: 64  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 123
           TAKS+RRR+QIELERDSIS+LNER+ R+HGKR+SSRTAMD+EEADRYIQMVKEQQQRGLQ
Sbjct: 64  TAKSSRRRDQIELERDSISLLNERILRFHGKRDSSRTAMDSEEADRYIQMVKEQQQRGLQ 123

Query: 124 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 183
           KLKGDRQR ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTE IEYVFIE
Sbjct: 124 KLKGDRQRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEAIEYVFIE 183

Query: 184 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 243
           YADGMAKLPVKQASRMLYRY+LPNE KRPRTLSKLNDT+TWEKRKTKG++AIQKMVVDLM
Sbjct: 184 YADGMAKLPVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLM 243

Query: 244 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 303
           ELYLHRLKQRR PYPKCSAME+F+AQFPYEPTVDQKEAF DVEKDLT RETPMDRLICGD
Sbjct: 244 ELYLHRLKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVEKDLTGRETPMDRLICGD 303

Query: 304 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 304 VGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363

Query: 364 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 423
           KAEKEKHL+MIKEGQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 364 KAEKEKHLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 423

Query: 424 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483
           +DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 424 VDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483

Query: 484 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543
           GGQVFYVLPRIKGLEEVK+FLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 484 GGQVFYVLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543

Query: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 603
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD ALE
Sbjct: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDDALE 603

Query: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663

Query: 664 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 723
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKI+NGAE+AAETDIW LMQFTENLRR 
Sbjct: 664 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRH 723

Query: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 783
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMET MNKKVFKLI+DSMTSEVH+
Sbjct: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETNMNKKVFKLISDSMTSEVHR 783

Query: 784 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCLSFEEH IKAGLLLELP+EQLLNWIFECLVELHAS PALIKY
Sbjct: 784 NCLSFEEHQIKAGLLLELPREQLLNWIFECLVELHASFPALIKY 827

BLAST of MS018689 vs. ExPASy Swiss-Prot
Match: F4JFJ3 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g02060 PE=2 SV=1)

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 652/828 (78.74%), Postives = 731/828 (88.29%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           M  L P PD  T  PLV  +L SFP   RLF  +  LRR  ++  S+  +V A   +   
Sbjct: 1   MMSLLPNPDPITV-PLV-LKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSAT 60

Query: 62  TAKSARRREQIEL-ERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGL 121
            AK  R RE+ EL E DSIS+LNER+RR  GKRE++R AMD+EEA++YI MVKEQQ+RGL
Sbjct: 61  AAKPTRWREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGL 120

Query: 122 QKLKGDRQRNESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEY 181
           QKLKG RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EY
Sbjct: 121 QKLKGIRQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEY 180

Query: 182 VFIEYADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMV 241
           VFIEYADGMAKLP+KQASR+LYRYNLPNETKRPRTLS+L+DTS WE+RKTKG+VAIQKMV
Sbjct: 181 VFIEYADGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMV 240

Query: 242 VDLMELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRL 301
           VDLMELYLHRL+Q+R PYPK   M DFAAQFPY  T DQK+AF DVEKDLTERETPMDRL
Sbjct: 241 VDLMELYLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRL 300

Query: 302 ICGDVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLS 361
           ICGDVGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLS
Sbjct: 301 ICGDVGFGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLS 360

Query: 362 RFQTKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIAS 421
           RFQTKAEKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIAS
Sbjct: 361 RFQTKAEKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIAS 420

Query: 422 FKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKY 481
           FKTS+DVLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLSSF KEKV  AIK 
Sbjct: 421 FKTSVDVLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKN 480

Query: 482 ELERGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKI 541
           EL+RGGQVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKI
Sbjct: 481 ELDRGGQVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKI 540

Query: 542 LICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD 601
           LICTNIVESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD
Sbjct: 541 LICTNIVESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD 600

Query: 602 QALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLS 661
           QALERL+ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLS
Sbjct: 601 QALERLSALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLS 660

Query: 662 KVDEHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTEN 721
           KV+E R+ SVPY  VKID++I P LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTEN
Sbjct: 661 KVEELRIFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTEN 720

Query: 722 LRRQHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTS 781
           LRRQ+GKEPYSMEI+LKKLYVRRMAADLG++RIYASGK V M+T M+KKVFKLITDSMT 
Sbjct: 721 LRRQYGKEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTC 780

Query: 782 EVHQNCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           +V+++ L +E   I A LLLELP+EQLLNW+F+CL ELHASLPALIKY
Sbjct: 781 DVYRSSLIYEGDQIMAELLLELPREQLLNWMFQCLSELHASLPALIKY 823

BLAST of MS018689 vs. ExPASy Swiss-Prot
Match: Q55750 (Transcription-repair-coupling factor OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=mfd PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 3.2e-153
Identity = 306/674 (45.40%), Postives = 432/674 (64.09%), Query Frame = 0

Query: 138  KVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIEYADGMAKLPVKQASRM 197
            +VD   L  GDYVVHK  GIG+F  +K D         EY+ I+YADG+ ++P       
Sbjct: 521  QVDINKLSPGDYVVHKSHGIGKF--LKLDALANR----EYLMIQYADGILRVPADSLD-S 580

Query: 198  LYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLMELYLHRLKQRRPPYPK 257
            L R+   +   RP  L K+     WE  K K R A++K+ VDL+ LY  R KQ    YP 
Sbjct: 581  LSRFR--HTGTRPPELHKMGG-KVWEATKNKVRKAVKKLAVDLLNLYAKRAKQVGYAYPP 640

Query: 258  CSA-MEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGDVGFGKTEVALRAIFG 317
             S   ++    FPY+PT DQ +A  DV++DL E + PMDRL+CGDVGFGKTEVA+RAIF 
Sbjct: 641  DSPWQQELEDSFPYQPTPDQLKAVQDVKRDL-EGDRPMDRLVCGDVGFGKTEVAVRAIFK 700

Query: 318  VVSSG-KQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHLDMIKEG 377
             V+SG KQ  +LAPT VL +QH+  + +RF+ +P + IGLL+RF+T +EK++ L  +K G
Sbjct: 701  AVTSGNKQVALLAPTTVLTQQHYHTLKERFAPYP-ITIGLLNRFRTASEKKEILAKLKSG 760

Query: 378  QLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSATPIPRT 437
            +LDI+VGT  +LG  V + +LGLLV+DEEQRFGV QKEKI + KT +DVLTL+ATPIPRT
Sbjct: 761  ELDIVVGTQQVLGTSVKFKDLGLLVIDEEQRFGVNQKEKIKTLKTEVDVLTLTATPIPRT 820

Query: 438  LYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQVFYVLPRIKGL 497
            LY++L+G R+ SLITTPPP R PIKTHLS ++ E +++AI+ EL+RGGQVFYV+PRI+G+
Sbjct: 821  LYMSLSGIREMSLITTPPPSRRPIKTHLSPYNPEVIRTAIRNELDRGGQVFYVVPRIEGI 880

Query: 498  EEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLDIQNANT 557
            EE+   L    P   IA+ HG+    +LE TM  F  G+  IL+CT I+E+GLDI   NT
Sbjct: 881  EELGGQLRQMVPSARIAIGHGQMEESELESTMLAFNDGEADILVCTTIIEAGLDIPRVNT 940

Query: 558  IIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEECRELGQG 617
            IIV+D Q+FGLAQLYQLRGRVGR+  +AHA+L YP++  L+++A  RL AL+E  +LG G
Sbjct: 941  IIVEDAQKFGLAQLYQLRGRVGRSGIQAHAWLLYPNQKQLTEKARLRLRALQEFSQLGSG 1000

Query: 618  FQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVPYQSVKID 677
            +QLA RDM IRG G + G +Q+G +  +G + + EML D++ ++    +  V  +  +ID
Sbjct: 1001 YQLATRDMEIRGVGNLLGAEQSGQMEAIGYEFYMEMLQDAIKEIQGQEIPKV--EDTQID 1060

Query: 678  VDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYSMEILLKK 737
            + +   +PS+YI  LE  M           TD   L +   +   ++G  P  +E L K 
Sbjct: 1061 LPLTAFIPSDYIPDLEEKMAAYRRITSIESTD--ELPKIALDWGDRYGMLPSPVEELFKV 1120

Query: 738  LYVRRMAADLGISRIYASGK-TVCMETKMNKKVFKLITDSMTSEVHQNCLSFEEHHIKAG 797
            + ++ +A  LG SRI   GK  + +ET M +  +KL+ +++ + +    +   +  +  G
Sbjct: 1121 VKLKHLAKSLGFSRIKVEGKQNLVLETPMEEPAWKLLAENLPNHLQSRFVYSAKKVVVRG 1178

Query: 798  LLLELPKEQLLNWI 809
            L +  P +Q+ N I
Sbjct: 1181 LGVLAPAKQMDNLI 1178

BLAST of MS018689 vs. ExPASy Swiss-Prot
Match: Q49V12 (Transcription-repair-coupling factor OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=mfd PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 6.2e-125
Identity = 247/617 (40.03%), Postives = 392/617 (63.53%), Query Frame = 0

Query: 144  LRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIEY-ADGMAKLPVKQASRMLYRYN 203
            L+ GDYVVH   G+GR++G++  ++ G     +Y+ ++Y       +PV Q  ++  +  
Sbjct: 497  LKVGDYVVHVHHGVGRYLGVE-TLEVGGVHK-DYIKLQYKGTDQLFVPVDQMDQV--QKY 556

Query: 204  LPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLMELYLHRLKQRRPPY-PKCSAM 263
            + +E K P+ L+KL  T  W+K K K + +++ M  +L+ELY  R       + P  +  
Sbjct: 557  VASEDKSPK-LNKLGGTE-WKKTKAKVQQSVEDMADELIELYKAREMSVGYKFGPDTAEQ 616

Query: 264  EDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGDVGFGKTEVALRAIFGVVSSG 323
             DF   FPYE T DQ ++  ++++D+ E E PMDRL+CGDVG+GKTEVA+RA F  V  G
Sbjct: 617  NDFEIDFPYELTPDQSKSIEEIKQDM-EIERPMDRLLCGDVGYGKTEVAVRAAFKAVMEG 676

Query: 324  KQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHLDMIKEGQLDIIV 383
            KQ   L PT +LA+QH+E + +R   FP +++ L+SRF+T  E ++  + +K G +DI+V
Sbjct: 677  KQVAFLVPTTILAQQHYETLIERMQDFP-IEVQLISRFRTTKEVKETKEGLKSGFVDIVV 736

Query: 384  GTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALT 443
            GTH LLG  + Y +LGLL+VDEEQRFGV+ KE+I S K ++DVLTL+ATPIPRTL++++ 
Sbjct: 737  GTHKLLGKDIQYKDLGLLIVDEEQRFGVRHKERIKSLKNNVDVLTLTATPIPRTLHMSML 796

Query: 444  GFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQVFYVLPRIKGLEEVKDF 503
            G RD S+I TPP  R P++T++   +   +K A++ EL R GQVFY+  +++ + E ++ 
Sbjct: 797  GVRDLSVIETPPENRFPVQTYVLEQNTNFIKEALERELSRDGQVFYLYNKVQSIYEKREQ 856

Query: 504  LEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDV 563
            L+   PD  I +AHG+   + LEETM +F   +  I++ T I+E+G+D+ NANT+I++D 
Sbjct: 857  LQMLMPDANIGVAHGQMNERDLEETMLSFINHEYDIIVTTTIIETGVDVPNANTLIIEDA 916

Query: 564  QQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAER 623
             +FGL+QLYQLRGRVGR+ +  +AY  +P   +LS+ A +RL A++E  ELG GF++A R
Sbjct: 917  DRFGLSQLYQLRGRVGRSSRIGYAYFLHPTNKVLSETAEDRLQAIKEFTELGSGFKIAMR 976

Query: 624  DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVPYQSVKIDVDIKPH 683
            D+ IRG G + G+QQ G + +VG DL+ +ML +++++    +        ++I+++I  +
Sbjct: 977  DLNIRGAGNLLGKQQHGFIDSVGFDLYSQMLEEAVNEKRGIKAEKQDAPEIEIELNIDAY 1036

Query: 684  LPSEYINYLENPMKIINGAEK-AAETDIWALMQFTENLRRQHGKEPYSMEILLKKLYVRR 743
            LP+EYI   ++ ++I     K  +ET    LM   + L  +    P  +E LL+ + ++ 
Sbjct: 1037 LPAEYIPNEQSKIEIYKKLRKIESETQ---LMDVKDELIDRFNDYPIEVERLLEMMEIKV 1096

Query: 744  MAADLGISRIYASGKTV 758
             A   G++ I   GK V
Sbjct: 1097 HALHAGVTLIKDVGKQV 1102

BLAST of MS018689 vs. ExPASy Swiss-Prot
Match: Q4L3G0 (Transcription-repair-coupling factor OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=mfd PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.6e-123
Identity = 257/666 (38.59%), Postives = 413/666 (62.01%), Query Frame = 0

Query: 108  YIQMVK-EQQQRGLQKLKGDRQRNESDGFNYKVDPY-TLRSGDYVVHKKVGIGRFVGIKF 167
            Y+Q+V   +++    K K  R+R ++     K+  Y  L  GDYVVH   G+GR++G++ 
Sbjct: 458  YMQLVVITERELFKTKQKKQRKRTKTLSNAEKIKSYQDLNVGDYVVHVHHGVGRYLGVE- 517

Query: 168  DVQKGSTEPIEYVFIEY-ADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEK 227
             ++ G     +Y+ ++Y       +PV Q  ++  +  + +E K P+ L+KL   S W+K
Sbjct: 518  TLEVGDVHR-DYIKLQYKGTDQLFVPVDQMDQV--QKYVASEDKSPK-LNKLGG-SEWKK 577

Query: 228  RKTKGRVAIQKMVVDLMELYLHRLKQRRPPYPKCSAMED-FAAQFPYEPTVDQKEAFSDV 287
             K K + +++ +  +L+ LY  R       Y + +A +  F   FPYE T DQ ++  ++
Sbjct: 578  TKAKVQQSVEDIADELIALYKEREMSVGYQYGEDTAEQSAFEMDFPYELTPDQAKSIDEI 637

Query: 288  EKDLTERETPMDRLICGDVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQ 347
            + D+ ERE PMDRL+CGDVG+GKTEVA+RA F  V  GKQ   L PT +LA+QH+E + +
Sbjct: 638  KGDM-ERERPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLIE 697

Query: 348  RFSSFPDVQIGLLSRFQTKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDE 407
            R   FP VQI L+SRF++  E ++  + +K G +DI+VGTH LLG  + Y +LGLL+VDE
Sbjct: 698  RMQDFP-VQIELISRFRSTKEVKETKEGLKSGYVDIVVGTHKLLGKDIHYKDLGLLIVDE 757

Query: 408  EQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHL 467
            EQRFGV+ KE+I + KT++DVLTL+ATPIPRTL++++ G RD S+I TPP  R P++T++
Sbjct: 758  EQRFGVRHKERIKTMKTNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTYV 817

Query: 468  SSFSKEKVKSAIKYELERGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQL 527
               +   +K A++ EL R GQVFY+  R++ + E ++ L+   PD  IA+AHG+   + L
Sbjct: 818  LEQNSNFIKEALERELSRDGQVFYLYNRVQSIYEKREQLQMLMPDANIAVAHGQMTERDL 877

Query: 528  EETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA 587
            EETM +F  G+  IL+ T I+E+G+D+ NANT+I+++  +FGL+QLYQLRGRVGR+ +  
Sbjct: 878  EETMLSFINGEFDILVTTTIIETGVDVPNANTLIIEEADRFGLSQLYQLRGRVGRSSRIG 937

Query: 588  HAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNV 647
            +AY  +    +L++ A ERL A++E  ELG GF++A RD+ IRG G + G+QQ G + +V
Sbjct: 938  YAYFLHSANKVLTETAEERLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDSV 997

Query: 648  GIDLFFEMLFDSLSKVDEHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKA 707
            G DL+ +ML +++++    +        V++++++  +LP+EYI   +  ++I     K 
Sbjct: 998  GFDLYSQMLEEAVNEKRGIKEEEPDAPEVEMELNLDAYLPAEYIQNEQAKIEIYKKLRKV 1057

Query: 708  AETDIWALMQFTENLRRQHGKEPYSMEILLKKLYVRRMAADLGISRIYASGK--TVCMET 767
               +   L    + L  +    P  +E LL+ + ++  A   G++ I   GK   V + T
Sbjct: 1058 ETEE--QLFDIKDELIDRFNDYPVEVERLLEMVEIKIHALHAGVTLIKDKGKQIEVSLST 1113

BLAST of MS018689 vs. ExPASy Swiss-Prot
Match: Q5HRQ2 (Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=mfd PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.2e-122
Identity = 251/654 (38.38%), Postives = 405/654 (61.93%), Query Frame = 0

Query: 108  YIQMVK-EQQQRGLQKLKGDRQRNESDGFNYKVDPY-TLRSGDYVVHKKVGIGRFVGIKF 167
            Y+Q+V   +++    + K  R+R ++     K+  Y  L  GDY+VH   G+GR++G++ 
Sbjct: 458  YMQLVVITERELFKTRQKKQRKRTKTISNAEKIKSYQDLNVGDYIVHVHHGVGRYLGVE- 517

Query: 168  DVQKGSTEPIEYVFIEY-ADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEK 227
             ++ G T   +Y+ ++Y       +PV Q  ++  +  + +E K PR L+KL  T  W+K
Sbjct: 518  TLEVGDTHR-DYIKLQYKGTDQLFVPVDQMDQV--QKYVASEDKSPR-LNKLGGTE-WKK 577

Query: 228  RKTKGRVAIQKMVVDLMELYLHRLKQRRPPYPKCSAMED-FAAQFPYEPTVDQKEAFSDV 287
             K K + +++ +  +L++LY  R       Y + +A +  F   FPYE T DQ ++  ++
Sbjct: 578  TKAKVQQSVEDIADELIDLYKEREMSVGYQYGQDTAEQSAFEHDFPYELTPDQSKSIDEI 637

Query: 288  EKDLTERETPMDRLICGDVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQ 347
            + D+ ER  PMDRL+CGDVG+GKTEVA+RA F  V  GKQ   L PT +LA+QH+E + +
Sbjct: 638  KGDM-ERARPMDRLLCGDVGYGKTEVAVRAAFKAVMDGKQVAFLVPTTILAQQHYETLLE 697

Query: 348  RFSSFPDVQIGLLSRFQTKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDE 407
            R   FP V+I L+SRF+T  E  +  + +K G +DI+VGTH LLG  + Y +LGLL+VDE
Sbjct: 698  RMQDFP-VEIQLVSRFRTAKEIRETKEGLKSGYVDIVVGTHKLLGKDIQYKDLGLLIVDE 757

Query: 408  EQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHL 467
            EQRFGV+ KE+I + K ++DVLTL+ATPIPRTL++++ G RD S+I TPP  R P++T++
Sbjct: 758  EQRFGVRHKERIKTLKKNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTYV 817

Query: 468  SSFSKEKVKSAIKYELERGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQL 527
               +   +K A++ EL R GQVFY+  +++ + E ++ L+   PD  IA+AHG+   + L
Sbjct: 818  LEQNTNFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQRLMPDANIAVAHGQMTERDL 877

Query: 528  EETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA 587
            EETM +F   +  IL+ T I+E+G+D+ NANT+I+++  +FGL+QLYQLRGRVGR+ +  
Sbjct: 878  EETMLSFINHEYDILVTTTIIETGVDVPNANTLIIEEADRFGLSQLYQLRGRVGRSSRIG 937

Query: 588  HAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNV 647
            +AY  +P   +L++ A ERL A++E  ELG GF++A RD+ IRG G + G+QQ G + +V
Sbjct: 938  YAYFLHPANKVLNETAEERLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDSV 997

Query: 648  GIDLFFEMLFDSLSKVDEHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKA 707
            G DL+ +ML +++++    +  S     +++++ +  +LP+EYI   +  ++I     K 
Sbjct: 998  GFDLYSQMLEEAVNEKRGIKEESPDAPDIEVELHLDAYLPAEYIQSEQAKIEIYKKLRKV 1057

Query: 708  AETDIWALMQFTENLRRQHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTV 758
               +   L    + L  +    P  +E LL  + ++  A   G+  I   GK++
Sbjct: 1058 ETEE--QLFDVKDELIDRFNDYPIEVERLLDIVEIKVHALHAGVELIKDKGKSI 1101

BLAST of MS018689 vs. ExPASy TrEMBL
Match: A0A6J1CJT5 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011831 PE=4 SV=1)

HSP 1 Score: 1613.2 bits (4176), Expect = 0.0e+00
Identity = 821/824 (99.64%), Postives = 823/824 (99.88%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG
Sbjct: 1   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 60

Query: 62  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 121
           TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ
Sbjct: 61  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 120

Query: 122 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181
           KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE
Sbjct: 121 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 180

Query: 182 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 241
           YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKG+VAIQKMVVDLM
Sbjct: 181 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGKVAIQKMVVDLM 240

Query: 242 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 301
           ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD
Sbjct: 241 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 300

Query: 302 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 361
           VGFGKTEVALRAIF VVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 301 VGFGKTEVALRAIFCVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 360

Query: 362 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 421
           KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 361 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 420

Query: 422 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 481
           IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 421 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 480

Query: 482 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 541
           GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 481 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 540

Query: 542 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 601
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE
Sbjct: 541 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 600

Query: 602 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 661
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 601 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 660

Query: 662 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 721
           HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ
Sbjct: 661 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 720

Query: 722 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 781
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMN+KVFKLITDSMTSEVHQ
Sbjct: 721 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNRKVFKLITDSMTSEVHQ 780

Query: 782 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY
Sbjct: 781 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 824

BLAST of MS018689 vs. ExPASy TrEMBL
Match: A0A1S3CNQ6 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503000 PE=4 SV=1)

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 759/824 (92.11%), Postives = 796/824 (96.60%), Query Frame = 0

Query: 4   LLSPAPDVSTFRPLVAFRLGSFPR--QWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 63
           L SPAPDVS+ RPL+AF+L SFP    W LFNRNCT R  P RC+SVTNV YAEDV+VPG
Sbjct: 4   LFSPAPDVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPG 63

Query: 64  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 123
           TAKSARRR+QIELERDSIS+LNER+ R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQ
Sbjct: 64  TAKSARRRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQ 123

Query: 124 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 183
           KLKGD+QR ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVFIE
Sbjct: 124 KLKGDKQRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIE 183

Query: 184 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 243
           YADGMAKLPVKQASRMLYRYNLPNE ++PRTLSKLNDT+TWEKRKTKG+VAIQKMVVDLM
Sbjct: 184 YADGMAKLPVKQASRMLYRYNLPNENRKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLM 243

Query: 244 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 303
           ELYLHRLKQRR PYPKCSAME+F+AQFPYEPTVDQKEAF DVE+DLTERETPMDRLICGD
Sbjct: 244 ELYLHRLKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGD 303

Query: 304 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 304 VGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363

Query: 364 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 423
           KAEKEKHL+MIKEGQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 364 KAEKEKHLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 423

Query: 424 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483
           +DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 424 VDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483

Query: 484 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543
           GGQ+FYVLPRIKGLEEVK+FLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 484 GGQIFYVLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543

Query: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 603
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALE
Sbjct: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALE 603

Query: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663
           RLAALE+CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 604 RLAALEDCRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663

Query: 664 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 723
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKI+NGAE+AAETDIW LMQFTENLRR 
Sbjct: 664 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRH 723

Query: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 783
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGK VCMET MNKKVF+LI+DSMTSEVH+
Sbjct: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHR 783

Query: 784 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCLSFEEH IKAGLLLELP+EQLLNW+FECLVELHASLPALIKY
Sbjct: 784 NCLSFEEHQIKAGLLLELPREQLLNWVFECLVELHASLPALIKY 827

BLAST of MS018689 vs. ExPASy TrEMBL
Match: A0A6J1GZL7 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111458673 PE=4 SV=1)

HSP 1 Score: 1506.1 bits (3898), Expect = 0.0e+00
Identity = 761/824 (92.35%), Postives = 794/824 (96.36%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           MALL P  DVS+ RPLVAFRLGSFP+ W LFNRNC LR   KRC+S+TNVVYAEDVMVPG
Sbjct: 1   MALLLPTADVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHFSKRCVSITNVVYAEDVMVPG 60

Query: 62  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 121
            AKSARRREQIELE DSI++LNER+RR+H KRESS+TAMDAEEADRYIQMVKEQQQRGLQ
Sbjct: 61  AAKSARRREQIELEHDSITVLNERIRRFHVKRESSKTAMDAEEADRYIQMVKEQQQRGLQ 120

Query: 122 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181
           KLKG+RQ+ ESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVFIE
Sbjct: 121 KLKGNRQKKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIE 180

Query: 182 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 241
           YADGMAKL VKQASRMLYRY+LPNE KRPRTLSKLNDT+TWEKRKTKG++AIQKMVVDLM
Sbjct: 181 YADGMAKLSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLM 240

Query: 242 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 301
           ELYLHRLKQRRPPYPKCSAME+FAAQF Y PTVDQKEAF DVEKDLTERETPMDRLICGD
Sbjct: 241 ELYLHRLKQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGD 300

Query: 302 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 361
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVIT RFSSFPDVQIGLLSRFQT
Sbjct: 301 VGFGKTEVALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITHRFSSFPDVQIGLLSRFQT 360

Query: 362 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 421
           K+EK+KHL+MIKEGQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 361 KSEKDKHLEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 420

Query: 422 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 481
           IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKV SAIKYELER
Sbjct: 421 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVNSAIKYELER 480

Query: 482 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 541
           GGQVFYV PRIKGLEEVKDFLEASFPDIEI LAHGKQYSKQLEETMENFALG IKILICT
Sbjct: 481 GGQVFYVFPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFALGAIKILICT 540

Query: 542 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 601
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALE
Sbjct: 541 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALE 600

Query: 602 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 661
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 601 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDE 660

Query: 662 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 721
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ
Sbjct: 661 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 720

Query: 722 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 781
           HGKEPYSMEILLKKLYVRRMAADLGI RIYASGKTVCMETKMNKKVFKLI++SM SEVH+
Sbjct: 721 HGKEPYSMEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHR 780

Query: 782 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCL+FEEH IKAGLLLELP+EQLLNWIFECLVELHASLPALIKY
Sbjct: 781 NCLTFEEHQIKAGLLLELPREQLLNWIFECLVELHASLPALIKY 824

BLAST of MS018689 vs. ExPASy TrEMBL
Match: A0A6J1JT22 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487597 PE=4 SV=1)

HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 759/824 (92.11%), Postives = 795/824 (96.48%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           MALL P  DVS+ RPLVAFRLGSFP+ W LFNRNC LR C KR +S+TNVVYAEDVMV G
Sbjct: 1   MALLLPTADVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHCSKRFVSITNVVYAEDVMVSG 60

Query: 62  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 121
           TAKSARRREQIELERDSI++LNER+RR+H KRESS+TAMDA EADRYIQMVKEQQQRGLQ
Sbjct: 61  TAKSARRREQIELERDSITVLNERIRRFHVKRESSKTAMDAVEADRYIQMVKEQQQRGLQ 120

Query: 122 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181
           KLKG+R++ ESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVFIE
Sbjct: 121 KLKGNRKKKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIE 180

Query: 182 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 241
           YADGMAKL VKQASRMLYRY+LPNE KRPRTLSKLNDT+TWEKRKTKG++AIQKMVVDLM
Sbjct: 181 YADGMAKLSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLM 240

Query: 242 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 301
           ELYLHRLKQRRPPYPKCSAME+FAAQF Y PTVDQKEAF DVEKDLTERETPMDRLICGD
Sbjct: 241 ELYLHRLKQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGD 300

Query: 302 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 361
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 301 VGFGKTEVALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 360

Query: 362 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 421
           K+EK+KHL+MIKEGQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 361 KSEKDKHLEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 420

Query: 422 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 481
           IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKVKSAIKYELER
Sbjct: 421 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSAFSKEKVKSAIKYELER 480

Query: 482 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 541
           GGQVFYV PRIKGLEEVKDFLEASFPDIEI LAHGKQYSKQLEETMENF+LG IKILICT
Sbjct: 481 GGQVFYVFPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFSLGAIKILICT 540

Query: 542 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 601
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALE
Sbjct: 541 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALE 600

Query: 602 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 661
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 601 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDE 660

Query: 662 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 721
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRR 
Sbjct: 661 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRH 720

Query: 722 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 781
           HGKEPYSMEILLKKLYVRRMAADLGI RIYASGKTVCMETKMNKKVFKLI++SM SEVH+
Sbjct: 721 HGKEPYSMEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHR 780

Query: 782 NCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           NCL+FEEH IKAGLLLELP+EQLLNWIFECLVELHASLPALIKY
Sbjct: 781 NCLTFEEHQIKAGLLLELPREQLLNWIFECLVELHASLPALIKY 824

BLAST of MS018689 vs. ExPASy TrEMBL
Match: A0A5A7VIC8 (ATP-dependent DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00090 PE=4 SV=1)

HSP 1 Score: 1447.6 bits (3746), Expect = 0.0e+00
Identity = 731/793 (92.18%), Postives = 764/793 (96.34%), Query Frame = 0

Query: 4   LLSPAPDVSTFRPLVAFRLGSFPR--QWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 63
           L SPAPDVS+ RPL+AF+L SFP    W LFNRNCT R  P RC+SVTNV YAEDV+VPG
Sbjct: 4   LFSPAPDVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPG 63

Query: 64  TAKSARRREQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQ 123
           TAKSARRR+QIELERDSIS+LNER+ R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQ
Sbjct: 64  TAKSARRRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQ 123

Query: 124 KLKGDRQRNESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 183
           KLKGD+QR ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVFIE
Sbjct: 124 KLKGDKQRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIE 183

Query: 184 YADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDLM 243
           YADGMAKLPVKQASRMLYRYNLPNE K+PRTLSKLNDT+TWEKRKTKG+VAIQKMVVDLM
Sbjct: 184 YADGMAKLPVKQASRMLYRYNLPNENKKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLM 243

Query: 244 ELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGD 303
           ELYLHRLKQRR PYPKCSAME+F+AQFPYEPTVDQKEAF DVE+DLTERETPMDRLICGD
Sbjct: 244 ELYLHRLKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGD 303

Query: 304 VGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363
           VGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT
Sbjct: 304 VGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQT 363

Query: 364 KAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTS 423
           KAEKEKHL+MIKEGQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS
Sbjct: 364 KAEKEKHLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTS 423

Query: 424 IDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483
           +DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER
Sbjct: 424 VDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELER 483

Query: 484 GGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543
           GGQ+FYVLPRIKGLEEVK+FLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT
Sbjct: 484 GGQIFYVLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICT 543

Query: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALE 603
           NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALE
Sbjct: 544 NIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALE 603

Query: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663
           RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE
Sbjct: 604 RLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDE 663

Query: 664 HRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQ 723
           HRVVSVPYQSVKID+DI PHLPSEYINYLENPMKI+NGAE+AAETDIW LMQFTENLRR 
Sbjct: 664 HRVVSVPYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRH 723

Query: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVHQ 783
           HGKEPYSMEILLKKLYVRRMAADLGISRIYASGK VCMET MNKKVF+LI+DSMTSEVH+
Sbjct: 724 HGKEPYSMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHR 783

Query: 784 NCLSFEEHHIKAG 795
           NCLSFEEH IK G
Sbjct: 784 NCLSFEEHQIKLG 796

BLAST of MS018689 vs. TAIR 10
Match: AT3G02060.1 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 652/828 (78.74%), Postives = 731/828 (88.29%), Query Frame = 0

Query: 2   MALLSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPG 61
           M  L P PD  T  PLV  +L SFP   RLF  +  LRR  ++  S+  +V A   +   
Sbjct: 1   MMSLLPNPDPITV-PLV-LKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSAT 60

Query: 62  TAKSARRREQIEL-ERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGL 121
            AK  R RE+ EL E DSIS+LNER+RR  GKRE++R AMD+EEA++YI MVKEQQ+RGL
Sbjct: 61  AAKPTRWREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGL 120

Query: 122 QKLKGDRQRNESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEY 181
           QKLKG RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EY
Sbjct: 121 QKLKGIRQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEY 180

Query: 182 VFIEYADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMV 241
           VFIEYADGMAKLP+KQASR+LYRYNLPNETKRPRTLS+L+DTS WE+RKTKG+VAIQKMV
Sbjct: 181 VFIEYADGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMV 240

Query: 242 VDLMELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRL 301
           VDLMELYLHRL+Q+R PYPK   M DFAAQFPY  T DQK+AF DVEKDLTERETPMDRL
Sbjct: 241 VDLMELYLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRL 300

Query: 302 ICGDVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLS 361
           ICGDVGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLS
Sbjct: 301 ICGDVGFGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLS 360

Query: 362 RFQTKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIAS 421
           RFQTKAEKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIAS
Sbjct: 361 RFQTKAEKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIAS 420

Query: 422 FKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKY 481
           FKTS+DVLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLSSF KEKV  AIK 
Sbjct: 421 FKTSVDVLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKN 480

Query: 482 ELERGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKI 541
           EL+RGGQVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKI
Sbjct: 481 ELDRGGQVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKI 540

Query: 542 LICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD 601
           LICTNIVESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD
Sbjct: 541 LICTNIVESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSD 600

Query: 602 QALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLS 661
           QALERL+ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLS
Sbjct: 601 QALERLSALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLS 660

Query: 662 KVDEHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTEN 721
           KV+E R+ SVPY  VKID++I P LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTEN
Sbjct: 661 KVEELRIFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTEN 720

Query: 722 LRRQHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTS 781
           LRRQ+GKEPYSMEI+LKKLYVRRMAADLG++RIYASGK V M+T M+KKVFKLITDSMT 
Sbjct: 721 LRRQYGKEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTC 780

Query: 782 EVHQNCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           +V+++ L +E   I A LLLELP+EQLLNW+F+CL ELHASLPALIKY
Sbjct: 781 DVYRSSLIYEGDQIMAELLLELPREQLLNWMFQCLSELHASLPALIKY 823

BLAST of MS018689 vs. TAIR 10
Match: AT3G02060.2 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 651/825 (78.91%), Postives = 730/825 (88.48%), Query Frame = 0

Query: 5   LSPAPDVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPGTAK 64
           L P PD  T  PLV  +L SFP   RLF  +  LRR  ++  S+  +V A   +    AK
Sbjct: 3   LLPNPDPITV-PLV-LKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSATAAK 62

Query: 65  SARRREQIEL-ERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKL 124
             R RE+ EL E DSIS+LNER+RR  GKRE++R AMD+EEA++YI MVKEQQ+RGLQKL
Sbjct: 63  PTRWREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGLQKL 122

Query: 125 KGDRQRNESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFI 184
           KG RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EYVFI
Sbjct: 123 KGIRQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEYVFI 182

Query: 185 EYADGMAKLPVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGRVAIQKMVVDL 244
           EYADGMAKLP+KQASR+LYRYNLPNETKRPRTLS+L+DTS WE+RKTKG+VAIQKMVVDL
Sbjct: 183 EYADGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMVVDL 242

Query: 245 MELYLHRLKQRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICG 304
           MELYLHRL+Q+R PYPK   M DFAAQFPY  T DQK+AF DVEKDLTERETPMDRLICG
Sbjct: 243 MELYLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICG 302

Query: 305 DVGFGKTEVALRAIFGVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQ 364
           DVGFGKTEVALRAIF VVS+GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLSRFQ
Sbjct: 303 DVGFGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLSRFQ 362

Query: 365 TKAEKEKHLDMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKT 424
           TKAEKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIASFKT
Sbjct: 363 TKAEKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIASFKT 422

Query: 425 SIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELE 484
           S+DVLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLSSF KEKV  AIK EL+
Sbjct: 423 SVDVLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKNELD 482

Query: 485 RGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC 544
           RGGQVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKILIC
Sbjct: 483 RGGQVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKILIC 542

Query: 545 TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL 604
           TNIVESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL
Sbjct: 543 TNIVESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL 602

Query: 605 ERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVD 664
           ERL+ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKV+
Sbjct: 603 ERLSALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVE 662

Query: 665 EHRVVSVPYQSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRR 724
           E R+ SVPY  VKID++I P LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTENLRR
Sbjct: 663 ELRIFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTENLRR 722

Query: 725 QHGKEPYSMEILLKKLYVRRMAADLGISRIYASGKTVCMETKMNKKVFKLITDSMTSEVH 784
           Q+GKEPYSMEI+LKKLYVRRMAADLG++RIYASGK V M+T M+KKVFKLITDSMT +V+
Sbjct: 723 QYGKEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTCDVY 782

Query: 785 QNCLSFEEHHIKAGLLLELPKEQLLNWIFECLVELHASLPALIKY 826
           ++ L +E   I A LLLELP+EQLLNW+F+CL ELHASLPALIKY
Sbjct: 783 RSSLIYEGDQIMAELLLELPREQLLNWMFQCLSELHASLPALIKY 822

BLAST of MS018689 vs. TAIR 10
Match: AT2G01440.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 216.5 bits (550), Expect = 8.2e-56
Identity = 145/416 (34.86%), Postives = 218/416 (52.40%), Query Frame = 0

Query: 262 EDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGDVGFGKTEVALRAIFGVVSSG 321
           + F    PY  T  Q  A S++  DL +R  PM+RL+ GDVG GKT VA  A   V+ SG
Sbjct: 512 KSFLKALPYSLTPSQLSAVSEIIWDL-KRPVPMNRLLQGDVGCGKTVVAFLACMEVIGSG 571

Query: 322 KQAMVLAPTIVLAKQHFEVITQRFSSFPDVQ----IGLLSRFQTKAEKEKHLDMIKEGQL 381
            QA  +APT +LA QH+E       +   V     IGLL+      +       ++ G +
Sbjct: 572 YQAAFMAPTELLAIQHYEQCRDLLENMEGVSSKPTIGLLTGSTPAKQSRMIRQDLQSGAI 631

Query: 382 DIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIAS--FKTSI------------- 441
             I+GTHSL+ +++ Y+ L + VVDE+QRFGV Q+ K  S  + TS+             
Sbjct: 632 SFIIGTHSLIAEKIEYSALRIAVVDEQQRFGVIQRGKFNSKLYGTSMISKSGSSDSDDTS 691

Query: 442 --------DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVK-- 501
                    VL +SATPIPR+L LAL G    + IT  P  R+P++TH+   ++  +K  
Sbjct: 692 KADLSMAPHVLAMSATPIPRSLALALYGDISLTQITGMPLGRIPVETHIFEGNETGIKEV 751

Query: 502 -SAIKYELERGGQVFYVLPRIKGLEEVKDFLEAS---------FPDIEIALAHGKQYSKQ 561
            S +  +L+ GG+V+ V P I   E++     AS         FP     L HG+  S  
Sbjct: 752 YSMMLEDLKSGGRVYVVYPVIDQSEQLPQLRAASAELEIVTKKFPKYNCGLLHGRMKSDD 811

Query: 562 LEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKE 621
            EE +  F  G+ +IL+ T ++E G+D+ +A+ ++V + ++FG+AQL+QLRGRVGR  ++
Sbjct: 812 KEEALNKFRSGETQILLSTQVIEIGVDVPDASMMVVMNAERFGIAQLHQLRGRVGRGTRK 871

Query: 622 AHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTG 639
           +        K LL   +   L  L    +   GF LA  D+ +RG G + G++Q+G
Sbjct: 872 S--------KCLLVGSSTNSLKRLNMLGKSSDGFYLANIDLLLRGPGDLLGKKQSG 918

BLAST of MS018689 vs. TAIR 10
Match: AT5G26742.1 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 54.3 bits (129), Expect = 5.4e-07
Identity = 77/302 (25.50%), Postives = 124/302 (41.06%), Query Frame = 0

Query: 325 MVLAPTIVLAKQHFEVITQR--FSSFPDVQIGLLSRFQTKAEKEKHLDMIKEGQLDIIVG 384
           +VLAPT  LAKQ  + I +   + S   V  G+    Q  A        +  G +D++VG
Sbjct: 184 LVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSA--------LTRG-VDVVVG 243

Query: 385 THSLLGDRVVYNNLGL-----LVVDE-EQRFGVKQKEKIASFKTSID------------- 444
           T   + D +   +L L     LV+DE +Q   V  +E + S   ++              
Sbjct: 244 TPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQSMLFSATMP 303

Query: 445 --VLTLSATPIPRTLYLALTGFRDASLITTPPPERVPI-KTHLSSFSKEKVKSAIKYELE 504
             V  L+   +   L + L G +D  L      E + +     +S SK  + S +     
Sbjct: 304 TWVKKLARKYLDNPLNIDLVGDQDEKL-----AEGIKLYAIATTSTSKRTILSDLITVYA 363

Query: 505 RGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC 564
           +GG+        +  +EV   L  S   I     HG     Q E T+  F  G   +L+ 
Sbjct: 364 KGGKTIVFTQTKRDADEVSLALSNS---IATEALHGDISQHQRERTLNAFRQGKFTVLVA 423

Query: 565 TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL 603
           T++   GLDI N + +I  ++       +++  GR GRA KE  A L +      + ++L
Sbjct: 424 TDVASRGLDIPNVDLVIHYELPNDPETFVHR-SGRTGRAGKEGSAILMHTSSQKRTVRSL 467

BLAST of MS018689 vs. TAIR 10
Match: AT5G26742.2 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 54.3 bits (129), Expect = 5.4e-07
Identity = 77/302 (25.50%), Postives = 124/302 (41.06%), Query Frame = 0

Query: 325 MVLAPTIVLAKQHFEVITQR--FSSFPDVQIGLLSRFQTKAEKEKHLDMIKEGQLDIIVG 384
           +VLAPT  LAKQ  + I +   + S   V  G+    Q  A        +  G +D++VG
Sbjct: 184 LVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSA--------LTRG-VDVVVG 243

Query: 385 THSLLGDRVVYNNLGL-----LVVDE-EQRFGVKQKEKIASFKTSID------------- 444
           T   + D +   +L L     LV+DE +Q   V  +E + S   ++              
Sbjct: 244 TPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQSMLFSATMP 303

Query: 445 --VLTLSATPIPRTLYLALTGFRDASLITTPPPERVPI-KTHLSSFSKEKVKSAIKYELE 504
             V  L+   +   L + L G +D  L      E + +     +S SK  + S +     
Sbjct: 304 TWVKKLARKYLDNPLNIDLVGDQDEKL-----AEGIKLYAIATTSTSKRTILSDLITVYA 363

Query: 505 RGGQVFYVLPRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILIC 564
           +GG+        +  +EV   L  S   I     HG     Q E T+  F  G   +L+ 
Sbjct: 364 KGGKTIVFTQTKRDADEVSLALSNS---IATEALHGDISQHQRERTLNAFRQGKFTVLVA 423

Query: 565 TNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQAL 603
           T++   GLDI N + +I  ++       +++  GR GRA KE  A L +      + ++L
Sbjct: 424 TDVASRGLDIPNVDLVIHYELPNDPETFVHR-SGRTGRAGKEGSAILMHTSSQKRTVRSL 467