MS017465 (gene) Bitter gourd (TR) v1

Overview


Name	MS017465
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold435: 695724 .. 696737 (+)
RNA-Seq Expression	MS017465
Synteny	MS017465

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAATTAATTTCAGAAAAATGGCAAAGCTTGCACATATCATTGTCTTCTTCATGGCTTCCATGTCCGCCGTTAGAGCAAACGACGTTCTTCTCGGCGTCTACTACGGTCTCTTCGGGGACAACGTGCCACCGCCGTGGAAGGTTGTGCAACTTTGCGTAAAATACAACATCCGGCGGATTCGACTGGACGAACCAAACCTTGAGGTCTTTGATGCATTTTGCGGCGCGGGAATTGACATATCCTTTGGCGTGCCCAACGACATGTTAATTAACATGAAAGCTAATGAATCCGCGGTGGTGGAGTGGTTCAATACCTATGTGGAACCCTACATCGGCGATTTTGTCATCAATTACATCATCGTTGGCAACAAAGCTATCCCGGGACTCGACGACTGCATATTGCCAGTCATGAAATCCCTTCAAAATCTCCTCAATGCCCGCTACCTTGGACAAGTGAAACTCACAACACTGGTTGGGTATAACGCCGCGTTGGCCGTTAAAGACCCTCCGTCCAGCGGCGCGTTCGATCCAAATGCTAGCGACAACATCAGAGAGATCCTACGATTCTTGTTGCGAGAAGGGTCGCCGCTGATGGTTAGTGTGTGCCCGTATACAAAATATGCGTATAGTAACAGGGCTATAAGTTTGAATTATGCAACATTTGGTGCGAAAACCCCAGTAGTTCACGACGGTGGGTTGAGTTATGACAACTTGTTTGATGCAATGGTGGATCCGTTTTATGCAGCGATGGATAAGTTAGGGGTCGGGGATGTGGATGTGGCGGTGGGAGAAACTGGGTGGCCGACGTGTGGAAACGGTAACATTGCAACCCCGCAGTATGCGTCGGCGTACAACAAAAATTTTAAGAGTCATATTAGCTCCGGCAGAGGGACGCCGAAGAGGCCAAATGTTTTGATTAGAGGATTCATTAAAAGTATGTTCAATGAGAATAAAATGCCGGAAGGAGAATCGCGGTGTTATGGAATATTCAATGTCGACTCTACACCTCTTTAT

mRNA sequence

TCAATTAATTTCAGAAAAATGGCAAAGCTTGCACATATCATTGTCTTCTTCATGGCTTCCATGTCCGCCGTTAGAGCAAACGACGTTCTTCTCGGCGTCTACTACGGTCTCTTCGGGGACAACGTGCCACCGCCGTGGAAGGTTGTGCAACTTTGCGTAAAATACAACATCCGGCGGATTCGACTGGACGAACCAAACCTTGAGGTCTTTGATGCATTTTGCGGCGCGGGAATTGACATATCCTTTGGCGTGCCCAACGACATGTTAATTAACATGAAAGCTAATGAATCCGCGGTGGTGGAGTGGTTCAATACCTATGTGGAACCCTACATCGGCGATTTTGTCATCAATTACATCATCGTTGGCAACAAAGCTATCCCGGGACTCGACGACTGCATATTGCCAGTCATGAAATCCCTTCAAAATCTCCTCAATGCCCGCTACCTTGGACAAGTGAAACTCACAACACTGGTTGGGTATAACGCCGCGTTGGCCGTTAAAGACCCTCCGTCCAGCGGCGCGTTCGATCCAAATGCTAGCGACAACATCAGAGAGATCCTACGATTCTTGTTGCGAGAAGGGTCGCCGCTGATGGTTAGTGTGTGCCCGTATACAAAATATGCGTATAGTAACAGGGCTATAAGTTTGAATTATGCAACATTTGGTGCGAAAACCCCAGTAGTTCACGACGGTGGGTTGAGTTATGACAACTTGTTTGATGCAATGGTGGATCCGTTTTATGCAGCGATGGATAAGTTAGGGGTCGGGGATGTGGATGTGGCGGTGGGAGAAACTGGGTGGCCGACGTGTGGAAACGGTAACATTGCAACCCCGCAGTATGCGTCGGCGTACAACAAAAATTTTAAGAGTCATATTAGCTCCGGCAGAGGGACGCCGAAGAGGCCAAATGTTTTGATTAGAGGATTCATTAAAAGTATGTTCAATGAGAATAAAATGCCGGAAGGAGAATCGCGGTGTTATGGAATATTCAATGTCGACTCTACACCTCTTTAT

Coding sequence (CDS)

TCAATTAATTTCAGAAAAATGGCAAAGCTTGCACATATCATTGTCTTCTTCATGGCTTCCATGTCCGCCGTTAGAGCAAACGACGTTCTTCTCGGCGTCTACTACGGTCTCTTCGGGGACAACGTGCCACCGCCGTGGAAGGTTGTGCAACTTTGCGTAAAATACAACATCCGGCGGATTCGACTGGACGAACCAAACCTTGAGGTCTTTGATGCATTTTGCGGCGCGGGAATTGACATATCCTTTGGCGTGCCCAACGACATGTTAATTAACATGAAAGCTAATGAATCCGCGGTGGTGGAGTGGTTCAATACCTATGTGGAACCCTACATCGGCGATTTTGTCATCAATTACATCATCGTTGGCAACAAAGCTATCCCGGGACTCGACGACTGCATATTGCCAGTCATGAAATCCCTTCAAAATCTCCTCAATGCCCGCTACCTTGGACAAGTGAAACTCACAACACTGGTTGGGTATAACGCCGCGTTGGCCGTTAAAGACCCTCCGTCCAGCGGCGCGTTCGATCCAAATGCTAGCGACAACATCAGAGAGATCCTACGATTCTTGTTGCGAGAAGGGTCGCCGCTGATGGTTAGTGTGTGCCCGTATACAAAATATGCGTATAGTAACAGGGCTATAAGTTTGAATTATGCAACATTTGGTGCGAAAACCCCAGTAGTTCACGACGGTGGGTTGAGTTATGACAACTTGTTTGATGCAATGGTGGATCCGTTTTATGCAGCGATGGATAAGTTAGGGGTCGGGGATGTGGATGTGGCGGTGGGAGAAACTGGGTGGCCGACGTGTGGAAACGGTAACATTGCAACCCCGCAGTATGCGTCGGCGTACAACAAAAATTTTAAGAGTCATATTAGCTCCGGCAGAGGGACGCCGAAGAGGCCAAATGTTTTGATTAGAGGATTCATTAAAAGTATGTTCAATGAGAATAAAATGCCGGAAGGAGAATCGCGGTGTTATGGAATATTCAATGTCGACTCTACACCTCTTTAT

Protein sequence

SINFRKMAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY

Homology

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BLAST of MS017465 vs. NCBI nr
Match: XP_022136187.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia])

HSP 1 Score: 684.1 bits (1764), Expect = 6.1e-193
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN 66
           MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN
Sbjct: 1   MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN 60

Query: 67  LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI 126
           LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI
Sbjct: 61  LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI 120

Query: 127 PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI 186
           PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI
Sbjct: 121 PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI 180

Query: 187 LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF 246
           LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF
Sbjct: 181 LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF 240

Query: 247 YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI 306
           YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI
Sbjct: 241 YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI 300

Query: 307 RGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           RGFIKSMFNENKMPEGES+CYGIFNVDSTPLY
Sbjct: 301 RGFIKSMFNENKMPEGESQCYGIFNVDSTPLY 332

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BLAST of MS017465 vs. NCBI nr
Match: XP_038880473.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 451.8 bits (1161), Expect = 5.1e-123
Identity = 219/336 (65.18%), Postives = 267/336 (79.46%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM----SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 66
           MAKLA II F MAS+    + VRA +VLLGV  GL GDN+PPPWK+VQLC KY+IRRIRL
Sbjct: 1   MAKLAQII-FLMASIIMAATIVRAYNVLLGVCLGLSGDNLPPPWKMVQLCKKYSIRRIRL 60

Query: 67  DEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVG 126
           DEPN++V +AF GA ID+SFG+PNDML +M  N++ V EWFNTY++P++ +F INY+IVG
Sbjct: 61  DEPNIDVLNAFRGAEIDLSFGIPNDMLTDMATNKALVEEWFNTYIKPFVNEFKINYVIVG 120

Query: 127 NKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 186
           +KAIPGLD CILPVM S Q+LLNA Y+GQVKLTTLVGYNAALA KDPPSSGAF P  S+N
Sbjct: 121 DKAIPGLDICILPVMMSFQDLLNANYIGQVKLTTLVGYNAALAYKDPPSSGAFHPTISEN 180

Query: 187 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 246
           +R IL+FL  EGSPLMVSV PY KY +    ISLNYA F    PVV DG L+Y NLFDAM
Sbjct: 181 MRGILKFLSEEGSPLMVSVFPYRKYMFDG-GISLNYAIFNETEPVVRDGELNYYNLFDAM 240

Query: 247 VDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRP 306
           V+  YAA+DK  VGDV V VGETGWPTCG+ + ATP  A  YNK F SHI SG+GTP++P
Sbjct: 241 VEAVYAAIDKEAVGDVTVVVGETGWPTCGSDDDATPAIAEEYNKKFVSHICSGKGTPRKP 300

Query: 307 NVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           NV + GFI+S+FNE++ P+G+S+CYG+F+++  P+Y
Sbjct: 301 NVFLEGFIQSIFNEDEKPQGDSQCYGMFDINMNPIY 334

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BLAST of MS017465 vs. NCBI nr
Match: XP_008451156.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo])

HSP 1 Score: 440.3 bits (1131), Expect = 1.5e-119
Identity = 214/337 (63.50%), Postives = 260/337 (77.15%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM----SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 66
           MAKL  IIVF MAS+    S VRA+DVLLG YYGL G+N+PPPWKVV LC KYNIRRIRL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 67  DEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVG 126
           D P ++V  AF G+ ID+SF VPNDML +M  N++ V EWFNTY++P++ DF INYIIVG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 127 NKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 186
           +KA+PGLD+ ILPVM SLQ+LLNA Y GQVKLTTLVGYNAAL  +DPPSSG F+P   +N
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 187 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 246
           +R IL+FL  EGS LMVSV P+ +Y  SN  +SLNYA      PVV DG L Y NLFDAM
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHRQYK-SNGGMSLNYAALKETKPVVEDGELKYSNLFDAM 240

Query: 247 VDPFYAAMDKLGVGDVDVAVGETGWPTCG-NGNIATPQYASAYNKNFKSHISSGRGTPKR 306
           +D FYAA++K  +G+V + VGETGWPTCG N  I T   A  YN  FKSHISSG+GTP++
Sbjct: 241 MDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRK 300

Query: 307 PNVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           PNV + GFI+S+FNEN+ PEGES+C+G+F+V+  P+Y
Sbjct: 301 PNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIY 336

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BLAST of MS017465 vs. NCBI nr
Match: XP_022968751.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima])

HSP 1 Score: 439.5 bits (1129), Expect = 2.6e-119
Identity = 215/333 (64.56%), Postives = 257/333 (77.18%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM-SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEP 66
           M K++H+I  FMA M   V A DVLLG YYGL GDN+  P  VVQLC KYNI R+RL+  
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 67  NLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKA 126
           N++V +AF G  ID+S GVPNDML NM  N++ V EWFNTYVEPY+ D++INYI+VG+KA
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKA 120

Query: 127 IPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIRE 186
           IPGLDD ILPVMKSLQ+LLNA Y G+ KLTTLVG+N ALAV +PPSSGAF+P   +N+R 
Sbjct: 121 IPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFN-ALAVTNPPSSGAFNPAIVENMRG 180

Query: 187 ILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDP 246
           IL FL  EGSPLMVSV  Y +Y Y     SL YAT   +  +V DG LSY+NLFD MVD 
Sbjct: 181 ILEFLRGEGSPLMVSVYTYDQYVYGGNR-SLGYATLSEQNSLVRDGMLSYNNLFDEMVDA 240

Query: 247 FYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVL 306
           FYAA+DK  VGDV + +GETGWPTCGN N  TP  A+ YN+NFKSHISSG+GTP++ N+ 
Sbjct: 241 FYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIY 300

Query: 307 IRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           I GFI+S+FNEN+ PEGESRCYG+FNVDSTP+Y
Sbjct: 301 IEGFIRSIFNENQKPEGESRCYGMFNVDSTPIY 331

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BLAST of MS017465 vs. NCBI nr
Match: KAG6590489.1 (putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.2 bits (1123), Expect = 1.3e-118
Identity = 216/327 (66.06%), Postives = 253/327 (77.37%), Query Frame = 0


Query: 13  IIVFFMASMS-AVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFD 72
           +I  FMA MS  V A DVLLG YYGL GDN+P P  VVQLC KYNI R+RL+ PNL+V +
Sbjct: 1   MIGLFMADMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIE 60

Query: 73  AFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDD 132
            F G  ID+SFGVPNDML NM  N++ V EWFNTYVEPY  D  INYI+VG+KAIPGLDD
Sbjct: 61  GFRGTEIDLSFGVPNDMLPNMATNKTLVEEWFNTYVEPYTNDCTINYIVVGDKAIPGLDD 120

Query: 133 CILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLL 192
            ILPVMKSLQ+LLNA Y G+VKLTTLVG+N ALAV +PPSSGAF+P   +N+R IL FL 
Sbjct: 121 NILPVMKSLQDLLNAHYFGEVKLTTLVGFN-ALAVTNPPSSGAFNPAIVENMRGILEFLR 180

Query: 193 REGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMD 252
            EGSPLMVSV  Y +Y Y    ISL YAT   +  +V DG LSY+NLFD +VD FYAAMD
Sbjct: 181 GEGSPLMVSVYTYDQYVYGGN-ISLGYATLSEQNTLVRDGMLSYNNLFDEIVDAFYAAMD 240

Query: 253 KLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIK 312
           K  VGDV +  GETGWP+CGN N  TP  A+ YN+NFK+HISSG+GTP++ N+ I GFI+
Sbjct: 241 KENVGDVAIVFGETGWPSCGNYNATTPLLAAEYNRNFKNHISSGKGTPRKENIYIEGFIR 300

Query: 313 SMFNENKMPEGESRCYGIFNVDSTPLY 339
           S+FNEN+ PEGESRCYG+FNVDSTP+Y
Sbjct: 301 SIFNENQKPEGESRCYGMFNVDSTPIY 325

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BLAST of MS017465 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.8e-70
Identity = 139/311 (44.69%), Postives = 193/311 (62.06%), Query Frame = 0


Query: 31  LGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCG-AGIDISFGVPNDML 90
           +G+ YGL GDN+P P KV+ L    +I +IR+ +PN EV +A  G   I ++ GV +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 91  INMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG-LDDCILPVMKSLQNLLNARY 150
             + A+E AV  WF T +EPY+ D  I +I VGN+ IPG +   +LPVM+SL NL+ +R 
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 151 LGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSVCPYTKYA 210
           L     T +  +N  L    PPS+G F   A + +  +L+ L +  SP++V + PY  YA
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 211 YSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVAVGETGWP 270
               +I L+YATF  +  VV DG L Y N+FDA+ D F  AM+K GV D+ + V ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 271 TCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMPEGESRCYG 330
           + GNGNI TP  A  YN+NF  HI+SG+GTPKRPN  I GF+ + FNEN+ P G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 331 IFNV-DSTPLY 339
           ++N  D  P+Y
Sbjct: 343 LYNPNDMKPIY 351

[Close]

BLAST of MS017465 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.9e-68
Identity = 137/332 (41.27%), Postives = 203/332 (61.14%), Query Frame = 0


Query: 10  LAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEV 69
           L+ I+++  ++ S        +G+ YGL GDN+P P  V+ L     I RIR+ +PN EV
Sbjct: 13  LSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEV 72

Query: 70  FDAFCG-AGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG 129
            +A  G   I+++ GV +  L  + A+E AV  WF   +E Y+ D  I +I VGN+ IPG
Sbjct: 73  LNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG 132

Query: 130 -LDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREIL 189
            +   +LPVM+SL NL+ +R L  + ++T+V   + L    PPS+G F   A + +  +L
Sbjct: 133 PIGPQVLPVMQSLTNLVKSRNL-PISISTVVAM-SNLEQSYPPSAGMFTSQAREQLVPVL 192

Query: 190 RFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFY 249
           + L +  +P++V++ PY  YA     I L+YA+F  K+ VV DG L Y N+FDA+ D F 
Sbjct: 193 KLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFV 252

Query: 250 AAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIR 309
            AM+K GV ++ + V ETGWP+ GNGN  TP  AS YN+NF  HI+SG+GTPKRPN  + 
Sbjct: 253 WAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMN 312

Query: 310 GFIKSMFNENKMPEGESRCYGIFN-VDSTPLY 339
           GF+ + FNEN+ P G  + +G++N  D  P+Y
Sbjct: 313 GFLFATFNENQKPAGTEQNFGLYNPSDMKPIY 342

[Close]

BLAST of MS017465 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 224.6 bits (571), Expect = 1.7e-57
Identity = 130/341 (38.12%), Postives = 186/341 (54.55%), Query Frame = 0


Query: 1   SINFRKMAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRI 60
           S++ +  A L  ++V       AV      +GV YG+ G+N+PPP +VV L    NI R+
Sbjct: 6   SLSIKGFALLVSVLV-------AVPTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARM 65

Query: 61  RLDEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYII 120
           RL +PN     A   + I +   VP   + ++ +N SA  +W    V  Y       YI 
Sbjct: 66  RLYDPNQAALQALRNSNIQVLLDVPRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIA 125

Query: 121 VGNKAIPGLD--DCILPVMKSLQNLLNARYL-GQVKLTTLVGYNAALAVKDPPSSGAFDP 180
           VGN+ IPG D    ILP M+++ N L++  L  Q+K++T V     L    PPS+GAF  
Sbjct: 126 VGNELIPGSDLAQYILPAMRNIYNALSSAGLQNQIKVSTAVD-TGVLGTSYPPSAGAFSS 185

Query: 181 NASDNIREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDN 240
            A   +  I++FL   G+PL+V+V PY  Y  +   ISL YA F A   VV DG  SY N
Sbjct: 186 AAQAYLSPIVQFLASNGAPLLVNVYPYFSYTGNPGQISLPYALFTASGVVVQDGRFSYQN 245

Query: 241 LFDAMVDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRG 300
           LFDA+VD  +AA++++G  +V V V E+GWP+ G G  A+   A  YN+N   H+  G G
Sbjct: 246 LFDAIVDAVFAALERVGGANVAVVVSESGWPSAGGGAEASTSNARTYNQNLIRHV--GGG 305

Query: 301 TPKRPNVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           TP+RP   I  +I  MFNEN+   G  + +G+F  +  P+Y
Sbjct: 306 TPRRPGKEIEAYIFEMFNENQKAGGIEQNFGLFYPNKQPVY 336

[Close]

BLAST of MS017465 vs. ExPASy Swiss-Prot
Match: P12257 (Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 5.2e-54
Identity = 116/308 (37.66%), Postives = 170/308 (55.19%), Query Frame = 0


Query: 31  LGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCGAGIDISFGVPNDMLI 90
           +GV YG+  +N+P    VV +     I+ +RL  PN     A  G GI++  G PND+L 
Sbjct: 7   IGVCYGMSANNLPAASTVVSMFKFNGIKSMRLYAPNQAALQAVGGTGINVVVGAPNDVLS 66

Query: 91  NMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDDCILPVMKSLQNLLNARYLG 150
           N+ A+ +A   W  + ++ Y       Y+ VGN+   G    ++P MK++   L A  LG
Sbjct: 67  NLAASPAAAASWVKSNIQAY-PKVSFRYVCVGNEVAGGATRNLVPAMKNVHGALVAAGLG 126

Query: 151 QVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSVCPYTKYAYS 210
            +K+TT V   A L V  PPS+G+F   A+  +  +++FL R  +PLM ++ PY  +AY+
Sbjct: 127 HIKVTTSVS-QAILGVFSPPSAGSFTGEAAAFMGPVVQFLARTNAPLMANIYPYLAWAYN 186

Query: 211 NRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVAVGETGWPTC 270
             A+ + YA F A   VV DG   Y NLFD  VD FY AM K G   V + V E+GWP+ 
Sbjct: 187 PSAMDMGYALFNASGTVVRDGAYGYQNLFDTTVDAFYTAMGKHGGSSVKLVVSESGWPS- 246

Query: 271 GNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMPEGESRCYGIF 330
           G G  ATP  A  YN++  +H+  GRGTP+ P   I  +I +MFNEN+   G  + +G+F
Sbjct: 247 GGGTAATPANARFYNQHLINHV--GRGTPRHPGA-IETYIFAMFNENQKDSGVEQNWGLF 306

Query: 331 NVDSTPLY 339
             +   +Y
Sbjct: 307 YPNMQHVY 308

[Close]

BLAST of MS017465 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 210.7 bits (535), Expect = 2.6e-53
Identity = 127/337 (37.69%), Postives = 185/337 (54.90%), Query Frame = 0


Query: 9   KLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLE 68
           ++A II+  +   S        +GV YG+ G+N+PP  +VVQL    NIRR+RL +PN  
Sbjct: 11  QMAAIILLGLLVSSTEIVGAQSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQA 70

Query: 69  VFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIP- 128
              A  G+ I++  GVPN  L N+ AN S    W    V  +       YI VGN+  P 
Sbjct: 71  ALQALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPV 130

Query: 129 ----GLDDCILPVMKSLQNLLNARYL-GQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 188
                L   +LP M++++N +++  L   +K+++ V     +    PPS G+F  +    
Sbjct: 131 TGTSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDM-TLIGNSFPPSQGSFRNDVRSF 190

Query: 189 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 248
           I  I+ F+ R  SPL+V++ PY  YA + R ISL YA F A   VV DG L Y NLFDAM
Sbjct: 191 IDPIIGFVRRINSPLLVNIYPYFSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAM 250

Query: 249 VDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRP 308
            D  YAA+ + G G +++ V E+GWP+ G    AT   A+ Y KN   H+   RG+P+RP
Sbjct: 251 SDAVYAALSRAGGGSIEIVVSESGWPSAG-AFAATTNNAATYYKNLIQHVK--RGSPRRP 310

Query: 309 NVLIRGFIKSMFNE-NKMPEGESRCYGIFNVDSTPLY 339
           N +I  ++ +MF+E NK PE E   +G+F+ +  P Y
Sbjct: 311 NKVIETYLFAMFDENNKNPELEKH-FGLFSPNKQPKY 342

[Close]

BLAST of MS017465 vs. ExPASy TrEMBL
Match: A0A6J1C4V7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.0e-193
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN 66
           MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN
Sbjct: 1   MAKLAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPN 60

Query: 67  LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI 126
           LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI
Sbjct: 61  LEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAI 120

Query: 127 PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI 186
           PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI
Sbjct: 121 PGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREI 180

Query: 187 LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF 246
           LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF
Sbjct: 181 LRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPF 240

Query: 247 YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI 306
           YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI
Sbjct: 241 YAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLI 300

Query: 307 RGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           RGFIKSMFNENKMPEGES+CYGIFNVDSTPLY
Sbjct: 301 RGFIKSMFNENKMPEGESQCYGIFNVDSTPLY 332

[Close]

BLAST of MS017465 vs. ExPASy TrEMBL
Match: A0A1S3BQX5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.4e-120
Identity = 214/337 (63.50%), Postives = 260/337 (77.15%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM----SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 66
           MAKL  IIVF MAS+    S VRA+DVLLG YYGL G+N+PPPWKVV LC KYNIRRIRL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 67  DEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVG 126
           D P ++V  AF G+ ID+SF VPNDML +M  N++ V EWFNTY++P++ DF INYIIVG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 127 NKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 186
           +KA+PGLD+ ILPVM SLQ+LLNA Y GQVKLTTLVGYNAAL  +DPPSSG F+P   +N
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 187 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 246
           +R IL+FL  EGS LMVSV P+ +Y  SN  +SLNYA      PVV DG L Y NLFDAM
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHRQYK-SNGGMSLNYAALKETKPVVEDGELKYSNLFDAM 240

Query: 247 VDPFYAAMDKLGVGDVDVAVGETGWPTCG-NGNIATPQYASAYNKNFKSHISSGRGTPKR 306
           +D FYAA++K  +G+V + VGETGWPTCG N  I T   A  YN  FKSHISSG+GTP++
Sbjct: 241 MDAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRK 300

Query: 307 PNVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           PNV + GFI+S+FNEN+ PEGES+C+G+F+V+  P+Y
Sbjct: 301 PNVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIY 336

[Close]

BLAST of MS017465 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.3e-119
Identity = 215/333 (64.56%), Postives = 257/333 (77.18%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM-SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEP 66
           M K++H+I  FMA M   V A DVLLG YYGL GDN+  P  VVQLC KYNI R+RL+  
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 67  NLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKA 126
           N++V +AF G  ID+S GVPNDML NM  N++ V EWFNTYVEPY+ D++INYI+VG+KA
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKA 120

Query: 127 IPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIRE 186
           IPGLDD ILPVMKSLQ+LLNA Y G+ KLTTLVG+N ALAV +PPSSGAF+P   +N+R 
Sbjct: 121 IPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFN-ALAVTNPPSSGAFNPAIVENMRG 180

Query: 187 ILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDP 246
           IL FL  EGSPLMVSV  Y +Y Y     SL YAT   +  +V DG LSY+NLFD MVD 
Sbjct: 181 ILEFLRGEGSPLMVSVYTYDQYVYGGNR-SLGYATLSEQNSLVRDGMLSYNNLFDEMVDA 240

Query: 247 FYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVL 306
           FYAA+DK  VGDV + +GETGWPTCGN N  TP  A+ YN+NFKSHISSG+GTP++ N+ 
Sbjct: 241 FYAAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIY 300

Query: 307 IRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           I GFI+S+FNEN+ PEGESRCYG+FNVDSTP+Y
Sbjct: 301 IEGFIRSIFNENQKPEGESRCYGMFNVDSTPIY 331

[Close]

BLAST of MS017465 vs. ExPASy TrEMBL
Match: A0A5A7UKS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001680 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 2.2e-116
Identity = 203/318 (63.84%), Postives = 248/318 (77.99%), Query Frame = 0


Query: 22  SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCGAGIDIS 81
           S VRA+DVLLG YYGL G+N+PPPWKVV LC KYNIRRIRLD P ++V  AF G+ ID+S
Sbjct: 9   SLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDLS 68

Query: 82  FGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPGLDDCILPVMKSLQ 141
           F VPNDML +M  N++ V EWFNTY++P++ DF INYIIVG+KA+PGLD+ ILPVM SLQ
Sbjct: 69  FSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQ 128

Query: 142 NLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSV 201
           +LLNA Y GQVKLTTLVGYNAAL  +DPPSSG F+P   +N+R IL+FL  EGS LMVSV
Sbjct: 129 DLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSV 188

Query: 202 CPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVA 261
            P+ +Y  SN  +SLNYA      PVV DG L Y NLFDAM+D FYAA++K  +G+V + 
Sbjct: 189 FPHRQYK-SNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLV 248

Query: 262 VGETGWPTCG-NGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMP 321
           VGETGWPTCG N  I T   A  YN  FKSHISSG+GTP++PNV + GFI+S+FNEN+ P
Sbjct: 249 VGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKP 308

Query: 322 EGESRCYGIFNVDSTPLY 339
           EGES+C+G+F+V+  P+Y
Sbjct: 309 EGESQCFGVFDVNMNPIY 325

[Close]

BLAST of MS017465 vs. ExPASy TrEMBL
Match: A0A0A0LZ10 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 2.1e-114
Identity = 208/336 (61.90%), Postives = 256/336 (76.19%), Query Frame = 0


Query: 7   MAKLAHIIVFFMASM----SAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 66
           MAK+   IVF MAS+    + V A D+LLG YYGL GDN+PPPWKVVQLC +YNIRR+RL
Sbjct: 1   MAKVVQ-IVFLMASILMAATVVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRL 60

Query: 67  DEPNLEVFDAFCGAGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVG 126
           D P+++V  AF G  ID+SFG+PN+ML ++  N+  V EWFNTYV+ ++ DF INYIIVG
Sbjct: 61  DVPDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVG 120

Query: 127 NKAIPGLDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDN 186
           +KAIP   + ILP+M SLQ+LLNA YLGQVKLTTLVGYNAAL  KDPPSSG FDP   ++
Sbjct: 121 DKAIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRES 180

Query: 187 IREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAM 246
           +R IL+FL  EGS LMVSV P+ KY + N  ISLNYA F    PVV D GL Y NLFDAM
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHQKYEF-NGGISLNYAIFNETNPVVQDEGLEYYNLFDAM 240

Query: 247 VDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRP 306
           VD FYAA++K  VG+V++ VGETGWPTCG+ +  T   A  YN  FKSHISSG GTP++P
Sbjct: 241 VDAFYAAIEKEMVGEVNIVVGETGWPTCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKP 300

Query: 307 NVLIRGFIKSMFNENKMPEGESRCYGIFNVDSTPLY 339
           NV + GFI+S+FNE+K PEG+S+CYG+F+V+  P+Y
Sbjct: 301 NVYLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIY 333

[Close]

BLAST of MS017465 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 267.7 bits (683), Expect = 1.3e-71
Identity = 139/311 (44.69%), Postives = 193/311 (62.06%), Query Frame = 0


Query: 31  LGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCG-AGIDISFGVPNDML 90
           +G+ YGL GDN+P P KV+ L    +I +IR+ +PN EV +A  G   I ++ GV +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 91  INMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG-LDDCILPVMKSLQNLLNARY 150
             + A+E AV  WF T +EPY+ D  I +I VGN+ IPG +   +LPVM+SL NL+ +R 
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 151 LGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSVCPYTKYA 210
           L     T +  +N  L    PPS+G F   A + +  +L+ L +  SP++V + PY  YA
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 211 YSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVAVGETGWP 270
               +I L+YATF  +  VV DG L Y N+FDA+ D F  AM+K GV D+ + V ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 271 TCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMPEGESRCYG 330
           + GNGNI TP  A  YN+NF  HI+SG+GTPKRPN  I GF+ + FNEN+ P G  + +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 331 IFNV-DSTPLY 339
           ++N  D  P+Y
Sbjct: 343 LYNPNDMKPIY 351

[Close]

BLAST of MS017465 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 260.4 bits (664), Expect = 2.0e-69
Identity = 137/332 (41.27%), Postives = 203/332 (61.14%), Query Frame = 0


Query: 10  LAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEV 69
           L+ I+++  ++ S        +G+ YGL GDN+P P  V+ L     I RIR+ +PN EV
Sbjct: 13  LSCIVLYVNSNNSGFVTAANSIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEV 72

Query: 70  FDAFCG-AGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG 129
            +A  G   I+++ GV +  L  + A+E AV  WF   +E Y+ D  I +I VGN+ IPG
Sbjct: 73  LNALRGHRDIEVTVGVKDQDLAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG 132

Query: 130 -LDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREIL 189
            +   +LPVM+SL NL+ +R L  + ++T+V   + L    PPS+G F   A + +  +L
Sbjct: 133 PIGPQVLPVMQSLTNLVKSRNL-PISISTVVAM-SNLEQSYPPSAGMFTSQAREQLVPVL 192

Query: 190 RFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFY 249
           + L +  +P++V++ PY  YA     I L+YA+F  K+ VV DG L Y N+FDA+ D F 
Sbjct: 193 KLLSQTSTPILVNIYPYFAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFV 252

Query: 250 AAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIR 309
            AM+K GV ++ + V ETGWP+ GNGN  TP  AS YN+NF  HI+SG+GTPKRPN  + 
Sbjct: 253 WAMEKEGVKNLPMVVSETGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMN 312

Query: 310 GFIKSMFNENKMPEGESRCYGIFN-VDSTPLY 339
           GF+ + FNEN+ P G  + +G++N  D  P+Y
Sbjct: 313 GFLFATFNENQKPAGTEQNFGLYNPSDMKPIY 342

[Close]

BLAST of MS017465 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.0e-69
Identity = 141/339 (41.59%), Postives = 198/339 (58.41%), Query Frame = 0


Query: 5   RKMAKLAHIIVFFMASMSAVRANDVL--LGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRL 64
           RK   L  + V F+ + S V   D    +G+ YGL GDN+PPP +V+ L    ++  IR+
Sbjct: 5   RKTFLLKFLCVAFLLNYSNVGFVDAATNIGLNYGLLGDNLPPPSEVINLYKSLSVTNIRI 64

Query: 65  DEPNLEVFDAFCG-AGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIV 124
            +   +V +AF G   I +   V N  L  +  +E AV  WF T +EPY+ D  I +I V
Sbjct: 65  FDTTTDVLNAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAV 124

Query: 125 GNKAIPG-LDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNAS 184
           GN+ IPG +   +LPVMKSL N++ +R L  +  TT+   N  L    PPS+G F P A 
Sbjct: 125 GNEVIPGEIGSYVLPVMKSLTNIVKSRSLPILISTTVAMTN--LGQSYPPSAGDFMPQAR 184

Query: 185 DNIREILRFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFD 244
           + +  +L+FL +  +P++V++ PY  YA     I L+YA F     VV DG L Y N+FD
Sbjct: 185 EQLTPVLKFLSQTNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVVQDGPLGYTNMFD 244

Query: 245 AMVDPFYAAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPK 304
            + D F  AM+K GV D+ + V ETGWP+ GNGN+ TP  AS YN NF  H+ SG+GTPK
Sbjct: 245 VIFDAFVWAMEKEGVKDLPMVVTETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPK 304

Query: 305 RPNVLIRGFIKSMFNENKMPEGESRCYGIFN-VDSTPLY 339
           RP   I GF+ + FNEN+ P G  + +G++N  D  P+Y
Sbjct: 305 RPKSGISGFLFATFNENQKPAGTEQNFGLYNPTDMKPIY 341

[Close]

BLAST of MS017465 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 8.8e-65
Identity = 134/315 (42.54%), Postives = 189/315 (60.00%), Query Frame = 0


Query: 10  LAHIIVFFMASMSAVRANDVLLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEV 69
           L+ I++ F  + S   A    +G+ YGL GDN P P  V+ L     I +IR+ +P  EV
Sbjct: 13  LSCIMLTFNYNTSGFVAAANSIGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEV 72

Query: 70  FDAFCG-AGIDISFGVPNDMLINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG 129
            +A  G   I+++ GV +  L  + ANE AV  WF T +EPY+ D  I +I VGN+ IPG
Sbjct: 73  LNALRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPG 132

Query: 130 -LDDCILPVMKSLQNLLNARYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREIL 189
            +   +LPVM+SL  L+ +  L  + ++T+V   + L    PPS+G F   A + +  +L
Sbjct: 133 PIGPQVLPVMQSLTILVKSMNL-PISISTVVAM-SNLEQSYPPSAGEFTSQAREQLVPVL 192

Query: 190 RFLLREGSPLMVSVCPYTKYAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFY 249
           + L +  +P++V++ PY  YA     I L+YATF  K  VV DG L Y N+FDA+ D F 
Sbjct: 193 KLLSQTSTPILVNIYPYFPYASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFV 252

Query: 250 AAMDKLGVGDVDVAVGETGWPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIR 309
            AM+K GV D+ + V ETGWP+ GNGN+ TP  A  YN+NF  HI SG+GTPKRPN  + 
Sbjct: 253 WAMEKEGVKDLPMVVSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMD 312

Query: 310 GFIKSMFNENKMPEG 323
           GF+ + FNEN+ P G
Sbjct: 313 GFLFATFNENQKPAG 325

[Close]

BLAST of MS017465 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 124/305 (40.66%), Postives = 184/305 (60.33%), Query Frame = 0


Query: 30  LLGVYYGLFGDNVPPPWKVVQLCVKYNIRRIRLDEPNLEVFDAFCG-AGIDISFGVPNDM 89
           ++G+ YGL GDN+P P  V++     N+ +IR+ EPN +V +A  G   I ++ G+ N+ 
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 90  LINMKANESAVVEWFNTYVEPYIGDFVINYIIVGNKAIPG--LDDCILPVMKSLQNLLNA 149
           L  + AN+ AV  WF+T ++PYI D  I +I VGN+AIPG      +LPV++SL +L+ +
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 150 RYLGQVKLTTLVGYNAALAVKDPPSSGAFDPNASDNIREILRFLLREGSPLMVSVCPYTK 209
           R L Q+ ++T V    +LA   PPS+G   P A   +  +LR L +  +P+ V++ PY  
Sbjct: 153 RNL-QISISTTV-TTTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYYF 212

Query: 210 YAYSNRAISLNYATFGAKTPVVHDGGLSYDNLFDAMVDPFYAAMDKLGVGDVDVAVGETG 269
           +A   + + L YA F     VV DG L Y NLFDA+ D F  AM+K GV  + + V ETG
Sbjct: 213 HASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETG 272

Query: 270 WPTCGNGNIATPQYASAYNKNFKSHISSGRGTPKRPNVLIRGFIKSMFNENKMPEGESRC 329
           WP+ GNG + TP     Y  NF  H++SG+GTPKRPN  I  +I   +NEN+ P G  + 
Sbjct: 273 WPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQH 332

Query: 330 YGIFN 332
           +G+++
Sbjct: 333 FGLYD 335

The following BLAST results are available for this feature:

BLAST of MS017465 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136187.1	6.1e-193	99.70	probable glucan endo-1,3-beta-glucosidase BG5 [Momordica charantia]	[more]
XP_038880473.1	5.1e-123	65.18	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
XP_008451156.1	1.5e-119	63.50	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG5 [Cucumis melo]	[more]
XP_022968751.1	2.6e-119	64.56	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima]	[more]
KAG6590489.1	1.3e-118	66.06	putative glucan endo-1,3-beta-glucosidase BG5, partial [Cucurbita argyrosperma s...	[more]

BLAST of MS017465 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	1.8e-70	44.69	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	2.9e-68	41.27	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	1.7e-57	38.12	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P12257	5.2e-54	37.66	Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P07979	2.6e-53	37.69	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]

BLAST of MS017465 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4V7	3.0e-193	99.70	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007943 ...	[more]
A0A1S3BQX5	7.4e-120	63.50	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492533 PE=3 SV...	[more]
A0A6J1HVR6	1.3e-119	64.56	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A5A7UKS6	2.2e-116	63.84	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0LZ10	2.1e-114	61.90	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G595900 PE=3...	[more]

BLAST of MS017465 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20340.1	1.3e-71	44.69	beta-1,3-glucanase 5	[more]
AT5G20330.1	2.0e-69	41.27	beta-1,3-glucanase 4	[more]
AT5G20390.1	2.0e-69	41.59	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	8.8e-65	42.54	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	1.1e-62	40.66	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 31..338 e-value: 2.9E-60 score: 204.5
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 258..271
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 31..338 e-value: 1.9E-91 score: 308.3
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 22..338
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 22..338
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 31..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017465.1	MS017465.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds