MS016976 (gene) Bitter gourd (TR) v1

Overview


Name	MS016976
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ATP synthase subunit alpha, chloroplastic
Location	scaffold197: 144870 .. 145069 (-)
RNA-Seq Expression	MS016976
Synteny	MS016976

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTAAAACTACAGTAGCCACAGATACGATTCTCAATCAACAAGGGGAAAATGTAATATGTGTTCATGTAGTTATTGGTCAAAAAACATCTTCTGTGGTTCAAGTAGTGACTACTTTACAGGAAATGAGGCAATGGAATACACTATTATAGTAGCTGAAACGGCGGATTCTCTGGCTACAGTACAATACCTTACTCCTTAT

mRNA sequence

GGTAAAACTACAGTAGCCACAGATACGATTCTCAATCAACAAGGGGAAAATGTAATATGTGTTCATGTAGTTATTGGTCAAAAAACATCTTCTGTGGTTCAAGTAGTGACTACTTTACAGGAAATGGCAATGGAATACACTATTATAGTAGCTGAAACGGCGGATTCTCTGGCTACAGTACAATACCTTACTCCTTAT

Coding sequence (CDS)

GGTAAAACTACAGTAGCCACAGATACGATTCTCAATCAACAAGGGGAAAATGTAATATGTGTTCATGTAGTTATTGGTCAAAAAACATCTTCTGTGGTTCAAGTAGTGACTACTTTACAGGAAATGGCAATGGAATACACTATTATAGTAGCTGAAACGGCGGATTCTCTGGCTACAGTACAATACCTTACTCCTTAT

Protein sequence

GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEMAMEYTIIVAETADSLATVQYLTPY

Homology

[Close]

BLAST of MS016976 vs. NCBI nr
Match: YP_009236266.1 (ATP synthase CF1 alpha subunit [Gynostemma pentaphyllum] >YP_009430612.1 ATP synthase CF1 alpha subunit [Gynostemma cardiospermum] >YP_009439789.1 ATP synthase CF1 alpha subunit [Gynostemma laxiflorum] >YP_009440142.1 ATP synthase CF1 alpha subunit [Gynostemma longipes] >YP_009440316.1 ATP synthase CF1 alpha subunit [Gynostemma pubescens] >QIT05723.1 CF1 subunit alpha [Gynostemma burmanicum (nom. inval.)] >AMF83996.1 ATP synthase CF1 alpha subunit [Gynostemma pentaphyllum] >ANI25170.1 ATP synthase CF1 alpha subunit [Gynostemma pentaphyllum] >ART64976.1 ATP synthase CF1 alpha subunit [Gynostemma pentaphyllum] >ART65063.1 ATP synthase CF1 alpha subunit [Gynostemma cardiospermum])

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. NCBI nr
Match: YP_009439876.1 (ATP synthase CF1 alpha subunit [Gynostemma caulopterum] >YP_009468846.1 ATP synthase CF1 alpha subunit [Gynostemma compressum] >ATG86729.1 ATP synthase CF1 alpha subunit [Gynostemma caulopterum] >AVA29820.1 ATP synthase CF1 alpha subunit [Gynostemma compressum])

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. NCBI nr
Match: YP_009439963.1 (ATP synthase CF1 alpha subunit [Gynostemma pentagynum] >ATG86816.1 ATP synthase CF1 alpha subunit [Gynostemma pentagynum])

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. NCBI nr
Match: YP_010015124.1 (ATP synthase CF1 alpha subunit [Gynostemma yixingense] >QOI14249.1 ATP synthase CF1 alpha subunit [Gynostemma yixingense])

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. NCBI nr
Match: YP_009454432.1 (ATP synthase CF1 alpha subunit [Daniellia pilosa] >AUF70219.1 ATP synthase CF1 alpha subunit [Daniellia pilosa])

HSP 1 Score: 101.3 bits (251), Expect = 3.3e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGKNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYLTPY
Sbjct: 235 LQYLTPY 241

[Close]

BLAST of MS016976 vs. ExPASy Swiss-Prot
Match: Q8S8Y3 (ATP synthase subunit alpha, chloroplastic OS=Atropa belladonna OX=33113 GN=atpA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy Swiss-Prot
Match: B1NWD5 (ATP synthase subunit alpha, chloroplastic OS=Manihot esculenta OX=3983 GN=atpA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy Swiss-Prot
Match: Q09X32 (ATP synthase subunit alpha, chloroplastic OS=Morus indica OX=248361 GN=atpA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy Swiss-Prot
Match: Q3C1H4 (ATP synthase subunit alpha, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=atpA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy Swiss-Prot
Match: Q33C53 (ATP synthase subunit alpha, chloroplastic OS=Nicotiana tomentosiformis OX=4098 GN=atpA PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy TrEMBL
Match: A0A109WWJ5 (ATP synthase subunit alpha, chloroplastic OS=Gynostemma pentaphyllum OX=182084 GN=atpA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy TrEMBL
Match: A0A2L0WR06 (ATP synthase subunit alpha, chloroplastic OS=Gynostemma compressum OX=1348125 GN=atpA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy TrEMBL
Match: A0A291IB12 (ATP synthase subunit alpha, chloroplastic OS=Gynostemma longipes OX=555431 GN=atpA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy TrEMBL
Match: A0A291IAW7 (ATP synthase subunit alpha, chloroplastic OS=Gynostemma pentagynum OX=555432 GN=atpA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. ExPASy TrEMBL
Match: A0A291IAL8 (ATP synthase subunit alpha, chloroplastic OS=Gynostemma caulopterum OX=1592219 GN=atpA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-18
Identity = 56/67 (83.58%), Postives = 60/67 (89.55%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQKTSSV QVVTTLQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKTSSVAQVVTTLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. TAIR 10
Match: ATCG00120.1 (ATP synthase subunit alpha )

HSP 1 Score: 98.2 bits (243), Expect = 2.6e-21
Identity = 54/67 (80.60%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1   GKTTVATDTILNQQGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIVAETADSLAT 60
           GKT VATDTILNQQG+NVICV+V IGQK SSV QVVT+LQE  AMEYTI+VAETADS AT
Sbjct: 175 GKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTSLQERGAMEYTIVVAETADSPAT 234

Query: 61  VQYLTPY 67
           +QYL PY
Sbjct: 235 LQYLAPY 241

[Close]

BLAST of MS016976 vs. TAIR 10
Match: AT2G07698.1 (ATPase, F1 complex, alpha subunit protein )

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-10
Identity = 38/76 (50.00%), Postives = 51/76 (67.11%), Query Frame = 0


Query: 1   GKTTVATDTILNQ---------QGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIV 60
           GKTT+A DTILNQ         + E + CV+V IGQK S+V Q++ TL+E  A+EY+I+V
Sbjct: 446 GKTTIAIDTILNQKQINSRATSESETMYCVYVAIGQKRSTVGQLIQTLEEANALEYSILV 505

Query: 61  AETADSLATVQYLTPY 67
           A TA   A +Q+L PY
Sbjct: 506 AATASDPAPLQFLAPY 521

[Close]

BLAST of MS016976 vs. TAIR 10
Match: ATMG01190.1 (ATP synthase subunit 1 )

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-10
Identity = 38/76 (50.00%), Postives = 51/76 (67.11%), Query Frame = 0


Query: 1   GKTTVATDTILNQ---------QGENVICVHVVIGQKTSSVVQVVTTLQEM-AMEYTIIV 60
           GKTT+A DTILNQ         + E + CV+V IGQK S+V Q++ TL+E  A+EY+I+V
Sbjct: 176 GKTTIAIDTILNQKQINSRATSESETMYCVYVAIGQKRSTVGQLIQTLEEANALEYSILV 235

Query: 61  AETADSLATVQYLTPY 67
           A TA   A +Q+L PY
Sbjct: 236 AATASDPAPLQFLAPY 251

The following BLAST results are available for this feature:

BLAST of MS016976 vs. NCBI nr


Match Name	E-value	Identity	Description
YP_009236266.1	2.5e-18	83.58	ATP synthase CF1 alpha subunit [Gynostemma pentaphyllum] >YP_009430612.1 ATP syn...	[more]
YP_009439876.1	2.5e-18	83.58	ATP synthase CF1 alpha subunit [Gynostemma caulopterum] >YP_009468846.1 ATP synt...	[more]
YP_009439963.1	2.5e-18	83.58	ATP synthase CF1 alpha subunit [Gynostemma pentagynum] >ATG86816.1 ATP synthase ...	[more]
YP_010015124.1	2.5e-18	83.58	ATP synthase CF1 alpha subunit [Gynostemma yixingense] >QOI14249.1 ATP synthase ...	[more]
YP_009454432.1	3.3e-18	83.58	ATP synthase CF1 alpha subunit [Daniellia pilosa] >AUF70219.1 ATP synthase CF1 a...	[more]

BLAST of MS016976 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8Y3	1.3e-20	82.09	ATP synthase subunit alpha, chloroplastic OS=Atropa belladonna OX=33113 GN=atpA ...	[more]
B1NWD5	1.3e-20	82.09	ATP synthase subunit alpha, chloroplastic OS=Manihot esculenta OX=3983 GN=atpA P...	[more]
Q09X32	1.3e-20	82.09	ATP synthase subunit alpha, chloroplastic OS=Morus indica OX=248361 GN=atpA PE=3...	[more]
Q3C1H4	1.3e-20	82.09	ATP synthase subunit alpha, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=atp...	[more]
Q33C53	1.3e-20	82.09	ATP synthase subunit alpha, chloroplastic OS=Nicotiana tomentosiformis OX=4098 G...	[more]

BLAST of MS016976 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A109WWJ5	1.2e-18	83.58	ATP synthase subunit alpha, chloroplastic OS=Gynostemma pentaphyllum OX=182084 G...	[more]
A0A2L0WR06	1.2e-18	83.58	ATP synthase subunit alpha, chloroplastic OS=Gynostemma compressum OX=1348125 GN...	[more]
A0A291IB12	1.2e-18	83.58	ATP synthase subunit alpha, chloroplastic OS=Gynostemma longipes OX=555431 GN=at...	[more]
A0A291IAW7	1.2e-18	83.58	ATP synthase subunit alpha, chloroplastic OS=Gynostemma pentagynum OX=555432 GN=...	[more]
A0A291IAL8	1.2e-18	83.58	ATP synthase subunit alpha, chloroplastic OS=Gynostemma caulopterum OX=1592219 G...	[more]

BLAST of MS016976 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00120.1	2.6e-21	80.60	ATP synthase subunit alpha	[more]
AT2G07698.1	1.2e-10	50.00	ATPase, F1 complex, alpha subunit protein	[more]
ATMG01190.1	1.2e-10	50.00	ATP synthase subunit 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000194	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	PFAM	PF00006	ATP-synt_ab	coord: 1..66 e-value: 6.6E-8 score: 32.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..66 e-value: 1.8E-14 score: 55.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 1..66
None	No IPR available	PANTHER	PTHR48082	ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL	coord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016976.1	MS016976.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005524	ATP binding