MS016864 (gene) Bitter gourd (TR) v1

BLAST of MS016864 vs. NCBI nr
Match: XP_022144381.1 (V-type proton ATPase subunit a2-like [Momordica charantia])

HSP 1 Score: 1584.3 bits (4101), Expect = 0.0e+00
Identity = 805/810 (99.38%), Postives = 807/810 (99.63%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH IKRCGE
Sbjct: 1   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH-IKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS
Sbjct: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH
Sbjct: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATN QIQNAMHKATYDSKSQ
Sbjct: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATN-QIQNAMHKATYDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF
Sbjct: 361 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLML+LSALIAVPWMLLPKPFLLKKQHEERH
Sbjct: 601 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 808

BLAST of MS016864 vs. NCBI nr
Match: KAA0065191.1 (Vacuolar proton ATPase [Cucumis melo var. makuwa])

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 730/810 (90.12%), Postives = 764/810 (94.32%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQL QLIIPNESA RTI YLGDLGLFQFNDLNAS+SPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTIFYLGDLGLFQFNDLNASKSPFQRTYAAQQIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS++SLG P  DLDNLEVKLGE E ELLEI DNNEKLQRN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSHSLGTPDFDLDNLEVKLGEFEVELLEIKDNNEKLQRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVL+KVGEFF LAQSIAAA QRELEVQQN EG ID+PLLLEQEMTTDP KQVKL
Sbjct: 121 ELLEYKLVLEKVGEFFHLAQSIAAAHQRELEVQQNGEGYIDTPLLLEQEMTTDPTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELKTTID+G+LH S+L QTIGH
Sbjct: 241 AKEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKTTIDMGQLHGSQLLQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQ+ M KAT DSKSQ
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQSVMQKATLDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL TKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLDTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRDTSCRDAT+IGL+KVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDTSCRDATSIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLI+VKW SGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGENQLFPGQKFLQL+L+LSAL AVPWML PKP LLKKQ+EERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPLLLKKQNEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYS++H TDDN E E  HGSH HEEFD SEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSVLHCTDDNHEIERHHGSHGHEEFDLSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGFNSL+IR VG+ VFI ATVGVLL+METLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFNSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSLLREEDE 809

BLAST of MS016864 vs. NCBI nr
Match: XP_022951118.1 (V-type proton ATPase subunit a2-like [Cucurbita moschata] >KAG6585562.1 V-type proton ATPase subunit a3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1447.6 bits (3746), Expect = 0.0e+00
Identity = 728/810 (89.88%), Postives = 768/810 (94.81%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGER
Sbjct: 181 GYISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGH
Sbjct: 241 AKNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQN + KAT+DSKSQ
Sbjct: 301 QYEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQNVIQKATFDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCR TSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++L+L+AL AVPWMLLPKPFLL+KQ+ ERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MS016864 vs. NCBI nr
Match: XP_038884965.1 (V-type proton ATPase subunit a2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1446.0 bits (3742), Expect = 0.0e+00
Identity = 729/810 (90.00%), Postives = 762/810 (94.07%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQL QLIIPNESAHR ISYLGDLGLFQFNDLNASRSPFQR YA  QIKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESAHRMISYLGDLGLFQFNDLNASRSPFQRAYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR++M +AGL PS+N +G P  DLDNLEVKL E E ELLEINDNNEKLQ N+S
Sbjct: 61  MARKLRFFREKMTKAGLLPSSNPIGAPDSDLDNLEVKLREFEVELLEINDNNEKLQHNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF  AQSIAAAQQRE EVQQN EGSID+PLLLEQEMTTD  KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHQAQSIAAAQQREQEVQQNGEGSIDTPLLLEQEMTTDTAKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK+KI KICEAFGANRYPFTDDLGKQ QMITEVSGKLSELKTTID+G+ HRSKL Q IG 
Sbjct: 241 AKDKIRKICEAFGANRYPFTDDLGKQFQMITEVSGKLSELKTTIDMGQRHRSKLLQMIGR 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQ+ M +AT DSKSQ
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQSVMQRATLDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL TKEAPPT+FRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLHTKEAPPTFFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFI REKKFSS+KLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFIIREKKFSSQKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRD+SCRDATTIGLIKV DTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDSSCRDATTIGLIKVHDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLI+VKW SGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGENQLFPGQKFLQL+L+LSAL AVPWML PKPFLLKKQ+EERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPFLLKKQNEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYS++ STDDN E E  HGSH HEEF+FSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSVLQSTDDNQEMEQHHGSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGFNSL+IR +G+ VF+CATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFNSLIIRILGIAVFVCATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSLLREEDE 808

BLAST of MS016864 vs. NCBI nr
Match: XP_023537097.1 (V-type proton ATPase subunit a2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1445.6 bits (3741), Expect = 0.0e+00
Identity = 726/810 (89.63%), Postives = 768/810 (94.81%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQ  QLI+PNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQFVQLIVPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGER
Sbjct: 181 GYISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGH
Sbjct: 241 AKNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQN + KAT+DSKSQ
Sbjct: 301 QYEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQNVIQKATFDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCR TSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++L+L+AL AVPWMLLPKPFLL+KQ+ ERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QG+SYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGRSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MS016864 vs. ExPASy Swiss-Prot
Match: Q9SJT7 (V-type proton ATPase subunit a2 OS=Arabidopsis thaliana OX=3702 GN=VHA-a2 PE=1 SV=1)

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 594/810 (73.33%), Postives = 705/810 (87.04%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSEPMQL Q+I+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  QIKRCGE
Sbjct: 15  MDLMRSEPMQLVQVIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAA-QIKRCGE 74

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARK+RFF++QM++AG++P         +DLD++EVKL ELEAEL+EIN NN+KLQR+++
Sbjct: 75  MARKIRFFKEQMSKAGVTPKETLDRENDIDLDDVEVKLEELEAELVEINANNDKLQRSYN 134

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           EL+EYKLVL+K GEFF  A   A AQQ E+E +Q  E  +++P LL++E + DP KQVKL
Sbjct: 135 ELVEYKLVLEKAGEFFASAHRSATAQQSEIETEQVGEDLLEAP-LLQEEKSVDPTKQVKL 194

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           G+++GLVPREKSM FERILFR+TRGN+++RQ+V++ SV DP SGEK EKNVFV+F+SGER
Sbjct: 195 GFLTGLVPREKSMVFERILFRATRGNIFIRQSVIEESVVDPNSGEKAEKNVFVVFYSGER 254

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK+KILKICEAFGANRYPF++DLGKQ QM+TEVSG+LSELKTTI  G   R+ L +TIG 
Sbjct: 255 AKSKILKICEAFGANRYPFSEDLGKQAQMMTEVSGRLSELKTTIGAGLDQRNILLETIGD 314

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           ++EQWNL ++KEK+IYHTLNMLS+DVTKKCLVGEGW PV A   +IQ+A+H+A  DS SQ
Sbjct: 315 KFEQWNLKIRKEKAIYHTLNMLSLDVTKKCLVGEGWSPVFAAT-EIQDALHRAAVDSNSQ 374

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           + +IF VLRTKE PPT+FRTNKFT++FQEIVDAYGVAKYQEANP V+TI+TFPFLFAVMF
Sbjct: 375 VGSIFQVLRTKEMPPTFFRTNKFTTAFQEIVDAYGVAKYQEANPSVFTIVTFPFLFAVMF 434

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLAT+Y I REKK SS+KLGDI+EM FGGRYVI MM+LFSIYTGLIYNEFF
Sbjct: 435 GDWGHGICLLLATMYLILREKKLSSQKLGDIMEMAFGGRYVIFMMSLFSIYTGLIYNEFF 494

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           S+P+ LF  SAY CRD SC +ATTIGLIK RDTYPFGVDP WHGTRSELPFLNSLKMKMS
Sbjct: 495 SIPYPLFASSAYDCRDVSCSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMS 554

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           IL+GVAQMNLGII+S+FNAKFF   +NIW+QFVPQMIFLN LFGYLS+LI++KWC+GSQA
Sbjct: 555 ILIGVAQMNLGIIMSFFNAKFFKSAVNIWFQFVPQMIFLNCLFGYLSVLIIIKWCTGSQA 614

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSP DDLGENQLFP QK +QL  +  AL++VPWMLLPKPF+LKKQHE RH
Sbjct: 615 DLYHVMIYMFLSPMDDLGENQLFPNQKIVQLTFLFLALVSVPWMLLPKPFILKKQHEARH 674

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QG SY+ +  TD++ + E + G H HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 675 QGLSYAQLDETDESLQVETNGGGHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLW 734

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFY+K+LL+AWGFN++ I  VG++VFI ATVGVLLVMETLSAFLHALRL
Sbjct: 735 ALSLAHSELSSVFYEKVLLMAWGFNNVFIWIVGILVFIFATVGVLLVMETLSAFLHALRL 794

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVE+QNKFY GDG+KF+PF+F+ + +EDE
Sbjct: 795 HWVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of MS016864 vs. ExPASy Swiss-Prot
Match: Q8W4S4 (V-type proton ATPase subunit a3 OS=Arabidopsis thaliana OX=3702 GN=VHA-a3 PE=1 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 601/811 (74.11%), Postives = 706/811 (87.05%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSE MQL QLI+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  QIKRCGE
Sbjct: 14  MDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAA-QIKRCGE 73

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARK+RFFRDQM++AG+           +DLD++EVKLGELEAEL+EIN NN+KLQR+++
Sbjct: 74  MARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVEINANNDKLQRSYN 133

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           EL+EYKLVLQK GEFF  A   AA QQRE E QQ  E  ++SP LL++E + D  KQVKL
Sbjct: 134 ELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESP-LLQEEKSIDSTKQVKL 193

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           G+++GLVPREKSM FERILFR+TRGN+++RQ V++  V DP SGEK EKNVFV+F+SGER
Sbjct: 194 GFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKAEKNVFVVFYSGER 253

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK+KILKICEAFGANRYPF++DLG+Q QMITEVSG+LSELKTTID G   R+ L QTIG 
Sbjct: 254 AKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAGLGQRNILLQTIGD 313

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           ++E WNL V+KEK+IYHTLNMLS+DVTKKCLV EGW PV A +++IQ+A+ +A  DS SQ
Sbjct: 314 KFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFA-SREIQDALQRAAVDSNSQ 373

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           + +IF VLRTKE+PPTYFRTNKFTS+ QEIVDAYGVAKYQEANPGV+TI+TFPFLFAVMF
Sbjct: 374 VGSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFTIVTFPFLFAVMF 433

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGIC+LLAT+Y I +EKK +S+KLGDI+EM FGGRYVI+MM+LFSIYTGLIYNEFF
Sbjct: 434 GDWGHGICILLATMYLILKEKKLASQKLGDIMEMAFGGRYVILMMSLFSIYTGLIYNEFF 493

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           S+PF LF PSAY CRD SC +ATTIGLIKVRDTYPFG+DP WHG+RSELPFLNSLKMKMS
Sbjct: 494 SIPFPLFAPSAYDCRDVSCSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMS 553

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGV+QMNLGII+SYFNA+FF  ++NIW+QF+PQMIFLNSLFGYLS+LI++KWC+GSQA
Sbjct: 554 ILLGVSQMNLGIIMSYFNARFFKSSVNIWFQFIPQMIFLNSLFGYLSVLIIIKWCTGSQA 613

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSP D+LGENQLFP QK LQL+L+  AL++VP MLLPKPF+LKKQHE RH
Sbjct: 614 DLYHVMIYMFLSPMDELGENQLFPHQKTLQLVLLFLALVSVPCMLLPKPFILKKQHEARH 673

Query: 662 QGQSYSLIHSTDDNDETE-HSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRL 721
           QGQ+Y+ +  TD++   E +  GSH HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRL
Sbjct: 674 QGQAYAPLDETDESLHVETNGGGSHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRL 733

Query: 722 WALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALR 781
           WALSLAHSELSSVFY+K+LLLAWG+N+ +I  VGV+VFI ATVGVLLVMETLSAFLHALR
Sbjct: 734 WALSLAHSELSSVFYEKVLLLAWGYNNPLILIVGVLVFIFATVGVLLVMETLSAFLHALR 793

Query: 782 LHWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           LHWVEFQNKFY GDG+KF+PF+F    +EDE
Sbjct: 794 LHWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of MS016864 vs. ExPASy Swiss-Prot
Match: Q8RWZ7 (V-type proton ATPase subunit a1 OS=Arabidopsis thaliana OX=3702 GN=VHA-a1 PE=2 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 4.3e-296
Identity = 503/807 (62.33%), Postives = 637/807 (78.93%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSE M L QLIIP ESAHR+I+YLG+LGL QF DLNA +SPFQRT+A +Q+KRCGE
Sbjct: 11  MDLMRSEKMTLVQLIIPVESAHRSITYLGELGLLQFRDLNADKSPFQRTFA-NQVKRCGE 70

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           M+RKLRFF+DQ+ +AGL  S      P + L +LE +L + E E+LE+N N+EKL++ ++
Sbjct: 71  MSRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLERQLADHEHEVLEMNSNSEKLRQTYN 130

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQREL-EVQQNKEGSIDSPLLLEQEMTTDPMKQVK 181
           ELLE+K+VL+K   F   + + A  ++ EL E   +  G I++  LLEQEM      Q  
Sbjct: 131 ELLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYSNNGFIETASLLEQEMNPGHSNQSG 190

Query: 182 LGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGE 241
           L +ISG++ ++K + FER+LFR+TRGNM   Q   D  + DP + E VEK VFV+FFSGE
Sbjct: 191 LRFISGIINKDKLLKFERMLFRATRGNMLFNQTTSDEEIMDPSTSEMVEKVVFVVFFSGE 250

Query: 242 RAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIG 301
           +A+ KILKICEAFGAN YP  +D  KQ Q+  EV  +LS+L+ T+D G  HR+    ++G
Sbjct: 251 QARTKILKICEAFGANCYPVPEDTTKQRQLTREVLSRLSDLEATLDAGTRHRNNALNSVG 310

Query: 302 HQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKS 361
           +    W   V++EK++Y TLNML+ DVTKKCLVGEGWCP  A   QI   + +AT+DS S
Sbjct: 311 YSLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEGWCPTFA-KTQIHEVLQRATFDSSS 370

Query: 362 QIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVM 421
           Q+  IFHV++  E+PPTYFRTNK T++FQEI+DAYGVA+YQEANP VY+++T+PFLFAVM
Sbjct: 371 QVGVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGVARYQEANPAVYSVVTYPFLFAVM 430

Query: 422 FGDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEF 481
           FGDWGHG+CLLL  LY + RE+K S++KLG  +EM FGGRYVI++MALFSIY GLIYNEF
Sbjct: 431 FGDWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLFGGRYVILLMALFSIYCGLIYNEF 490

Query: 482 FSVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKM 541
           FSVPF +FG SAY CRDT+C DA T+GLIK RD YPFGVDP W G+R+ELP+LNSLKMKM
Sbjct: 491 FSVPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKM 550

Query: 542 SILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQ 601
           SILLG+AQMNLG+ILS+FNA+FFG +++I YQF+PQMIFLNSLFGYLSLLI++KWC+GSQ
Sbjct: 551 SILLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQMIFLNSLFGYLSLLIIIKWCTGSQ 610

Query: 602 ADLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEER 661
           ADLYH+MIYMFLSPT++LGEN+LF GQ+ LQ++L+L A IAVPWML PKPF L+K H ER
Sbjct: 611 ADLYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLLAFIAVPWMLFPKPFALRKIHMER 670

Query: 662 HQGQSYSLIHSTD---DNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASY 721
            QG++Y ++ S++   D +      G H  EEF+FSE+FVHQLIH+IEFVLG+VSNTASY
Sbjct: 671 FQGRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSEIFVHQLIHSIEFVLGSVSNTASY 730

Query: 722 LRLWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLH 781
           LRLWALSLAHSELS+VFY+K+LLLAWG+ +++IR +GV VF  AT  +LL+METLSAFLH
Sbjct: 731 LRLWALSLAHSELSTVFYEKVLLLAWGYENILIRLIGVAVFAFATAFILLMMETLSAFLH 790

Query: 782 ALRLHWVEFQNKFYAGDGFKFSPFSFS 805
           ALRLHWVEF  KF+ GDG+KF PFSF+
Sbjct: 791 ALRLHWVEFMGKFFNGDGYKFKPFSFA 815

BLAST of MS016864 vs. ExPASy Swiss-Prot
Match: Q54E04 (Vacuolar proton translocating ATPase 100 kDa subunit OS=Dictyostelium discoideum OX=44689 GN=vatM PE=1 SV=2)

HSP 1 Score: 644.0 bits (1660), Expect = 2.2e-183
Identity = 380/830 (45.78%), Postives = 532/830 (64.10%), Query Frame = 0

Query: 6   RSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGEMARK 65
           RS PMQ+ QL +  E+AH T+  LG LGL QF D N   + FQR +  +++KRC +M +K
Sbjct: 10  RSSPMQMVQLFVQIEAAHDTVDELGKLGLIQFLDDNEHVNLFQRNF-VNEVKRCDDMEKK 69

Query: 66  LRFFRDQMAR----AGLSPSTNSLGVPGLD--LDNLEVKLGELEAELLEINDNNEKLQRN 125
           L+FF DQ+ +      L P  N L V   D  +D LE +  ELE+EL ++N N E LQRN
Sbjct: 70  LKFFEDQVKKEPKLQKLLPD-NMLSVVDDDSQMDELEGRFDELESELKQVNANQETLQRN 129

Query: 126 HSELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQ- 185
           ++EL++ + VL K   FFQ        +   L   +  E S  SPLL E +  ++  KQ 
Sbjct: 130 YNELIQLRHVLTKDSVFFQ--------ENPNLIEGEGHEHSARSPLLAEDQHVSEVAKQG 189

Query: 186 VKLGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFS 245
           VKLG+I+G++  +K   F+R L+R+TRGN Y++ A ++  + DP +GE+  K VF++FF 
Sbjct: 190 VKLGFITGVMNTDKMPQFQRSLWRTTRGNNYVKDARIEEEIIDPQTGEETAKTVFIVFFQ 249

Query: 246 GERAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQT 305
           GER + KI KICE+FGAN Y   D+  ++  ++ +V+ ++++L   +   K H+ +    
Sbjct: 250 GERLQQKIKKICESFGANIYDCPDNSFERSNLLQKVTVRITDLYEVLQRSKDHKRQTLAG 309

Query: 306 IGHQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDS 365
           I  +   W   V  EKSIYHT+N+   DV +KCL+ +GW P     ++IQ A+  AT  S
Sbjct: 310 IVPRLYSWKKKVLLEKSIYHTMNLFDYDVGRKCLIAKGWTP-KDKIEEIQLALRTATTRS 369

Query: 366 KSQIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFA 425
            + + ++  +++T+ +PPT+F TNK+TSSFQEIV+AYG+A Y+E NP V TI+TFPFLF 
Sbjct: 370 GALVPSVLSIIKTEGSPPTHFETNKYTSSFQEIVNAYGIAHYREVNPAVLTIVTFPFLFG 429

Query: 426 VMFGDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYN 485
           VMFGD GHG  LLL+ L  I+ EKK + +KL ++I+M F GRYV+ +M+LFSIY G IYN
Sbjct: 430 VMFGDVGHGALLLLSALGLISLEKKLAGKKLNELIQMPFDGRYVLFLMSLFSIYVGFIYN 489

Query: 486 EFFSVPFELFGPSAYGCRDTSCRDATTIGLIKVRDT---YPFGVDPKWHGTRSELPFLNS 545
           E FS+P  +FG S Y    T+       GL   + T   YP GVDP W G  +EL + NS
Sbjct: 490 ECFSIPMNIFG-SQYNLNSTT-------GLYTYQHTDRVYPVGVDPLWKGAPNELVYYNS 549

Query: 546 LKMKMSILLGVAQMNLGI---ILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIL 605
            KMK+SI+ GV QM++GI   +L+Y N K   + +NI  QFVPQMIFL S+FGY+S+LI+
Sbjct: 550 FKMKLSIIFGVVQMSVGICFSLLNYLNQKGPIKIVNILTQFVPQMIFLWSIFGYMSVLII 609

Query: 606 VKWC---SGSQAD------LYHIMIYMFLSP--TDDLGENQLFPGQKFLQLMLVLSALIA 665
           +KW       + D      +   +I MFLSP  T D+     F GQ  +Q  L+  ALI+
Sbjct: 610 LKWVVPYRSFEVDKVDPPFILPTIIAMFLSPGGTPDV---VFFSGQGAVQTALLFLALIS 669

Query: 666 VPWMLLPKPFLLKKQHEERHQGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLI 725
           +P ML+ KP  +K+ H +  + +   L H  +++D+ E  +  H  EEF+  EVFVHQ+I
Sbjct: 670 IPVMLVIKPLFMKRFHFQ--EVERKKLGHHEEEHDD-EALYTGHHGEEFEMGEVFVHQVI 729

Query: 726 HTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICA 785
           HTIEFVLGAVSNTASYLRLWALSLAHSELSSVF+++IL+      +  +  VG   ++ A
Sbjct: 730 HTIEFVLGAVSNTASYLRLWALSLAHSELSSVFWERILIGQVERGNPFLAFVGFGAWLGA 789

Query: 786 TVGVLLVMETLSAFLHALRLHWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           +V VLL+ME+LSAFLHALRLHWVEFQNKFY GDG +F P+S + +  ED+
Sbjct: 790 SVAVLLLMESLSAFLHALRLHWVEFQNKFYIGDGVRFIPYSATRILSEDD 814

BLAST of MS016864 vs. ExPASy Swiss-Prot
Match: A1A5G6 (V-type proton ATPase 116 kDa subunit a1 OS=Xenopus tropicalis OX=8364 GN=atp6v0a1 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.8e-177
Identity = 361/853 (42.32%), Postives = 517/853 (60.61%), Query Frame = 0

Query: 3   DLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGEM 62
           +L RSE M LAQL + +E+A+  +S LG+LG  QF DLN   + FQR +  ++++RC EM
Sbjct: 3   ELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKF-VNEVRRCEEM 62

Query: 63  ARKLRFFRDQMARAGLS--PSTNSLGVP-GLDLDNLEVKLGELEAELLEINDNNEKLQRN 122
            RKLRF   ++ +A +S   +  +  VP   D+ +LE    ++E EL EIN N E L+RN
Sbjct: 63  DRKLRFVEKEVKKANISILDTGENPEVPFPRDMIDLEANFEKIEIELKEINTNQEALKRN 122

Query: 123 HSELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQ- 182
             EL E K +L+K  +FF                    +   D  LL E     +P +  
Sbjct: 123 FLELTELKFILRKTQQFF--------------------DEMADPDLLEESSSLLEPSEMG 182

Query: 183 ----VKLGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFV 242
               ++LG+++G++ RE+   FER+L+R  RGN++LRQA ++  + DPV+G+ V K+VF+
Sbjct: 183 RGAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDSVHKSVFI 242

Query: 243 IFFSGERAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSK 302
           IFF G++ KN++ KICE F A+ YP  +   ++ +M T V+ ++ +L+  ++  + HR +
Sbjct: 243 IFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMATGVNTRIEDLQMVLNQTEDHRQR 302

Query: 303 LFQTIGHQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKA 362
           + Q        W + V+K K+IYHTLN+ ++DVT+KCL+ E WCPV A    IQ A+ + 
Sbjct: 303 VLQAAAKSLRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPV-ADLDSIQFALRRG 362

Query: 363 TYDSKSQIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFP 422
           T  S S + +I + ++T + PPTY +TNKFT  FQ +VDAYG+  Y+E NP  YTIITFP
Sbjct: 363 TEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNLVDAYGIGSYREINPAPYTIITFP 422

Query: 423 FLFAVMFGDWGHGICLLLATLYFITREKKFSSEKL-GDIIEMTFGGRYVIMMMALFSIYT 482
           FLFAVMFGD+GHGI + L  ++ + RE +  S+K+  ++  M F GRY+I++M LFSIYT
Sbjct: 423 FLFAVMFGDFGHGILMTLFAVWMVVRESRILSQKIDNELFTMMFSGRYIILLMGLFSIYT 482

Query: 483 GLIYNEFFSVPFELFGPSAYGCR----DTSCRDA---TTIGLIKVRDT------YPFGVD 542
           GLIYN+ FS    LFG S++  R    DT   D    T++  +    T      YPFG+D
Sbjct: 483 GLIYNDCFSKALNLFG-SSWSVRPMFTDTWSEDLLKHTSVLQLNPNVTGVFNGPYPFGID 542

Query: 543 PKWHGTRSELPFLNSLKMKMSILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFL 602
           P W    ++L FLNS KMKMS++LG+  M  G+ LS  N  +F + +NI+  F+P+MIF+
Sbjct: 543 PIWSLATNKLTFLNSFKMKMSVVLGIIHMTFGVALSLLNHIYFKKPLNIYLGFIPEMIFM 602

Query: 603 NSLFGYLSLLILVKWCSGSQA------DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLML 662
            +LFGYL +LI+ KWC+   +       L    I MFL    D     L+ GQ  LQ  L
Sbjct: 603 TTLFGYLVILIIYKWCAYDASTSMVAPSLLIHFINMFLFSYQDTSLPMLYKGQMGLQCFL 662

Query: 663 VLSALIAVPWMLLPKPFLLKKQH-EERHQG-QSYSLIH-----STDDNDETEHSHGSHSH 722
           V+ A+I VPWML+ KP +L++Q+   +H G  ++  I      + +D +  +H   S   
Sbjct: 663 VVCAIICVPWMLVVKPLILRRQYLRRKHLGTHNFGGIRVGNGPTEEDAEIIQHDQLSMHS 722

Query: 723 EE---------FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKI 782
           EE         FDF +  VHQ IHTIE+ LG +SNTASYLRLWALSLAH++LS V +  +
Sbjct: 723 EEGEEPAMEEVFDFGDTVVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMV 782

Query: 783 LLLAWGFNSLMIRAVGVVV---FICATVGVLLVMETLSAFLHALRLHWVEFQNKFYAGDG 809
           + +     SL      V V   F   T+ +LL+ME LSAFLHALRLHWVEFQNKFY G G
Sbjct: 783 MHVGLSIRSLGGGIALVFVFSAFATLTIAILLIMEGLSAFLHALRLHWVEFQNKFYMGTG 832

BLAST of MS016864 vs. ExPASy TrEMBL
Match: A0A6J1CT88 (V-type proton ATPase subunit a OS=Momordica charantia OX=3673 GN=LOC111014074 PE=3 SV=1)

HSP 1 Score: 1584.3 bits (4101), Expect = 0.0e+00
Identity = 805/810 (99.38%), Postives = 807/810 (99.63%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH IKRCGE
Sbjct: 1   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATH-IKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS
Sbjct: 61  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH
Sbjct: 241 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATN QIQNAMHKATYDSKSQ
Sbjct: 301 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATN-QIQNAMHKATYDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF
Sbjct: 361 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTY FGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYLFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLML+LSALIAVPWMLLPKPFLLKKQHEERH
Sbjct: 601 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLLLSALIAVPWMLLPKPFLLKKQHEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 808

BLAST of MS016864 vs. ExPASy TrEMBL
Match: A0A5A7VHH1 (V-type proton ATPase subunit a OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005280 PE=3 SV=1)

HSP 1 Score: 1453.3 bits (3761), Expect = 0.0e+00
Identity = 730/810 (90.12%), Postives = 764/810 (94.32%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQL QLIIPNESA RTI YLGDLGLFQFNDLNAS+SPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTIFYLGDLGLFQFNDLNASKSPFQRTYAAQQIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS++SLG P  DLDNLEVKLGE E ELLEI DNNEKLQRN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSHSLGTPDFDLDNLEVKLGEFEVELLEIKDNNEKLQRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVL+KVGEFF LAQSIAAA QRELEVQQN EG ID+PLLLEQEMTTDP KQVKL
Sbjct: 121 ELLEYKLVLEKVGEFFHLAQSIAAAHQRELEVQQNGEGYIDTPLLLEQEMTTDPTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELKTTID+G+LH S+L QTIGH
Sbjct: 241 AKEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKTTIDMGQLHGSQLLQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQ+ M KAT DSKSQ
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQSVMQKATLDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL TKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLDTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRDTSCRDAT+IGL+KVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDTSCRDATSIGLVKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLI+VKW SGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGENQLFPGQKFLQL+L+LSAL AVPWML PKP LLKKQ+EERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPLLLKKQNEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYS++H TDDN E E  HGSH HEEFD SEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSVLHCTDDNHEIERHHGSHGHEEFDLSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGFNSL+IR VG+ VFI ATVGVLL+METLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFNSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSLLREEDE 809

BLAST of MS016864 vs. ExPASy TrEMBL
Match: A0A6J1GGT7 (V-type proton ATPase subunit a OS=Cucurbita moschata OX=3662 GN=LOC111454061 PE=3 SV=1)

HSP 1 Score: 1447.6 bits (3746), Expect = 0.0e+00
Identity = 728/810 (89.88%), Postives = 768/810 (94.81%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTVSYLGDLGLFQFNDLNASRSPFQRTYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS+NSL V   DLDNLEVKLGE EAELLEIN NNEKL+RN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSNSLAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF LAQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHLAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLV REKSMAFERILFRSTRGN+YLRQAVVDGSVTDPVSGEKVEKNVFVIF+SGER
Sbjct: 181 GYISGLVLREKSMAFERILFRSTRGNVYLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGH
Sbjct: 241 AKNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQN + KAT+DSKSQ
Sbjct: 301 QYEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQNVIQKATFDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCR TSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++L+L+AL AVPWMLLPKPFLL+KQ+ ERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYSL+H TDDN ET+  H S SH++FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSLLHGTDDNLETKQHHSSPSHDDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGF+S  IR +G+ VFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFDSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MS016864 vs. ExPASy TrEMBL
Match: A0A0A0LLK1 (V-type proton ATPase subunit a OS=Cucumis sativus OX=3659 GN=Csa_2G364570 PE=3 SV=1)

HSP 1 Score: 1444.1 bits (3737), Expect = 0.0e+00
Identity = 730/810 (90.12%), Postives = 763/810 (94.20%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQL QLIIPNESA RTISYLGDLGLFQFNDLNAS+SPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQLVQLIIPNESARRTISYLGDLGLFQFNDLNASKSPFQRTYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QM RAGLSPS+ SLG    DLDNLEVKLGELE ELLEI DNNEKLQRN+S
Sbjct: 61  MARKLRFFREQMTRAGLSPSSYSLGTHDFDLDNLEVKLGELEVELLEIKDNNEKLQRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF LAQ  AAA QRELEVQQN EGSID+PLLLEQEMTTDP KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHLAQRTAAAHQRELEVQQNGEGSIDTPLLLEQEMTTDPTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLVPREKSMAFERILFRSTRGN+YLRQAV+DGSVTDPVSG+KVEKNVFVIF+SGER
Sbjct: 181 GYISGLVPREKSMAFERILFRSTRGNVYLRQAVIDGSVTDPVSGDKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK KI KICEAFGANRYPFTDDLGKQ QMITEVS KLSELK TID+G+LHRS+L QTIGH
Sbjct: 241 AKEKIRKICEAFGANRYPFTDDLGKQFQMITEVSRKLSELKITIDMGQLHRSQLLQTIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYE WNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV AT  QIQ+ M KAT+DSKSQ
Sbjct: 301 QYELWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFAT-IQIQSVMQKATHDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL TKEAPPTYF TNKFTSSFQEIVDAYGVAKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLDTKEAPPTYFCTNKFTSSFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLYFI REKKFS +KLGDI+EMTFGGRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYFIIREKKFSGQKLGDIVEMTFGGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFGPSAYGCRDTSCRDAT+IGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGPSAYGCRDTSCRDATSIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFFGE+INIWYQFVPQMIFLNSLFGYLSLLI+VKW SGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFGESINIWYQFVPQMIFLNSLFGYLSLLIIVKWYSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGENQLFPGQKFLQL+L+LSAL AVPWML PKPFLLKKQ+EERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENQLFPGQKFLQLLLLLSALTAVPWMLFPKPFLLKKQNEERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYS++H TDDN E E  HGSH HEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSVLHCTDDNHEIERHHGSHGHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGF+SL+IR VG+ VFI ATVGVLL+METLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFDSLIIRIVGMAVFIFATVGVLLIMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSPFSFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPFSFSLLREEDE 808

BLAST of MS016864 vs. ExPASy TrEMBL
Match: A0A6J1KIY1 (V-type proton ATPase subunit a OS=Cucurbita maxima OX=3661 GN=LOC111496148 PE=3 SV=1)

HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 725/810 (89.51%), Postives = 766/810 (94.57%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDLLRSEPMQ  QLIIPNES HRT+SYLGDLGLFQFNDLNASRSPFQRTYA  QIKRCGE
Sbjct: 1   MDLLRSEPMQFVQLIIPNESVHRTLSYLGDLGLFQFNDLNASRSPFQRTYAA-QIKRCGE 60

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARKLRFFR+QMARAGLSPS+NS  V   DLDNLEVKLGE EAELLEIN NNEKL+RN+S
Sbjct: 61  MARKLRFFREQMARAGLSPSSNSQAVADFDLDNLEVKLGEFEAELLEINGNNEKLRRNYS 120

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           ELLEYKLVLQKVGEFF +AQSIAAAQQRELEVQQN EGSIDSPLLLEQEMTTD  KQVKL
Sbjct: 121 ELLEYKLVLQKVGEFFHIAQSIAAAQQRELEVQQNGEGSIDSPLLLEQEMTTDSTKQVKL 180

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           GYISGLV REKSMAFERILFRSTRGN++LRQAVVDGSVTDPVSGEKVEKNVFVIF+SGER
Sbjct: 181 GYISGLVLREKSMAFERILFRSTRGNVHLRQAVVDGSVTDPVSGEKVEKNVFVIFYSGER 240

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AKNKILKI EAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTID+G++HRSKL Q IGH
Sbjct: 241 AKNKILKISEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDLGQVHRSKLLQMIGH 300

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           QYEQWNLLVKKEKS+YHTLNMLSVDVTKKCLVGEGWCPV ATN QIQ+ + KAT+DSKSQ
Sbjct: 301 QYEQWNLLVKKEKSVYHTLNMLSVDVTKKCLVGEGWCPVFATN-QIQSVIQKATFDSKSQ 360

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           IEAIFHVL+TKEAPPTYFRTNKFTS+FQEIVDAYG+AKYQEANPGVYTI+TFPFLFAVMF
Sbjct: 361 IEAIFHVLQTKEAPPTYFRTNKFTSTFQEIVDAYGIAKYQEANPGVYTIVTFPFLFAVMF 420

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLATLY I REKKFSS+KLGDI+EMTF GRYVIMMMALFSIYTGLIYNEFF
Sbjct: 421 GDWGHGICLLLATLYLIIREKKFSSQKLGDIVEMTFAGRYVIMMMALFSIYTGLIYNEFF 480

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           SVPFELFG SAYGCR TSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS
Sbjct: 481 SVPFELFGRSAYGCRHTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 540

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGVAQMNLGIILSYFNAKFF E INIWYQFVPQMIFLNSLFGYLSLLI+VKWCSGSQA
Sbjct: 541 ILLGVAQMNLGIILSYFNAKFFEEKINIWYQFVPQMIFLNSLFGYLSLLIIVKWCSGSQA 600

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSPTDDLGEN+LFPGQ+F+Q++L+L+AL AVPWMLLPKPFLL+KQ+ ERH
Sbjct: 601 DLYHVMIYMFLSPTDDLGENELFPGQQFVQVLLLLAALTAVPWMLLPKPFLLRKQNAERH 660

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QGQSYSL+H TDDN ET+  HGS SH +FDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 661 QGQSYSLLHGTDDNLETKQHHGSPSHNDFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 720

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFYDK+LLLAWGFNS  IR +G+ VFICATVGVLLVMETLSAFLHALRL
Sbjct: 721 ALSLAHSELSSVFYDKVLLLAWGFNSWSIRIIGIAVFICATVGVLLVMETLSAFLHALRL 780

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVEFQNKFYAGDGFKFSP+SFS LR+EDE
Sbjct: 781 HWVEFQNKFYAGDGFKFSPYSFSLLREEDE 808

BLAST of MS016864 vs. TAIR 10
Match: AT2G21410.1 (vacuolar proton ATPase A2 )

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 594/810 (73.33%), Postives = 705/810 (87.04%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSEPMQL Q+I+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  QIKRCGE
Sbjct: 15  MDLMRSEPMQLVQVIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAA-QIKRCGE 74

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARK+RFF++QM++AG++P         +DLD++EVKL ELEAEL+EIN NN+KLQR+++
Sbjct: 75  MARKIRFFKEQMSKAGVTPKETLDRENDIDLDDVEVKLEELEAELVEINANNDKLQRSYN 134

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           EL+EYKLVL+K GEFF  A   A AQQ E+E +Q  E  +++P LL++E + DP KQVKL
Sbjct: 135 ELVEYKLVLEKAGEFFASAHRSATAQQSEIETEQVGEDLLEAP-LLQEEKSVDPTKQVKL 194

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           G+++GLVPREKSM FERILFR+TRGN+++RQ+V++ SV DP SGEK EKNVFV+F+SGER
Sbjct: 195 GFLTGLVPREKSMVFERILFRATRGNIFIRQSVIEESVVDPNSGEKAEKNVFVVFYSGER 254

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK+KILKICEAFGANRYPF++DLGKQ QM+TEVSG+LSELKTTI  G   R+ L +TIG 
Sbjct: 255 AKSKILKICEAFGANRYPFSEDLGKQAQMMTEVSGRLSELKTTIGAGLDQRNILLETIGD 314

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           ++EQWNL ++KEK+IYHTLNMLS+DVTKKCLVGEGW PV A   +IQ+A+H+A  DS SQ
Sbjct: 315 KFEQWNLKIRKEKAIYHTLNMLSLDVTKKCLVGEGWSPVFAAT-EIQDALHRAAVDSNSQ 374

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           + +IF VLRTKE PPT+FRTNKFT++FQEIVDAYGVAKYQEANP V+TI+TFPFLFAVMF
Sbjct: 375 VGSIFQVLRTKEMPPTFFRTNKFTTAFQEIVDAYGVAKYQEANPSVFTIVTFPFLFAVMF 434

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGICLLLAT+Y I REKK SS+KLGDI+EM FGGRYVI MM+LFSIYTGLIYNEFF
Sbjct: 435 GDWGHGICLLLATMYLILREKKLSSQKLGDIMEMAFGGRYVIFMMSLFSIYTGLIYNEFF 494

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           S+P+ LF  SAY CRD SC +ATTIGLIK RDTYPFGVDP WHGTRSELPFLNSLKMKMS
Sbjct: 495 SIPYPLFASSAYDCRDVSCSEATTIGLIKTRDTYPFGVDPVWHGTRSELPFLNSLKMKMS 554

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           IL+GVAQMNLGII+S+FNAKFF   +NIW+QFVPQMIFLN LFGYLS+LI++KWC+GSQA
Sbjct: 555 ILIGVAQMNLGIIMSFFNAKFFKSAVNIWFQFVPQMIFLNCLFGYLSVLIIIKWCTGSQA 614

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSP DDLGENQLFP QK +QL  +  AL++VPWMLLPKPF+LKKQHE RH
Sbjct: 615 DLYHVMIYMFLSPMDDLGENQLFPNQKIVQLTFLFLALVSVPWMLLPKPFILKKQHEARH 674

Query: 662 QGQSYSLIHSTDDNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRLW 721
           QG SY+ +  TD++ + E + G H HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRLW
Sbjct: 675 QGLSYAQLDETDESLQVETNGGGHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRLW 734

Query: 722 ALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALRL 781
           ALSLAHSELSSVFY+K+LL+AWGFN++ I  VG++VFI ATVGVLLVMETLSAFLHALRL
Sbjct: 735 ALSLAHSELSSVFYEKVLLMAWGFNNVFIWIVGILVFIFATVGVLLVMETLSAFLHALRL 794

Query: 782 HWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           HWVE+QNKFY GDG+KF+PF+F+ + +EDE
Sbjct: 795 HWVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of MS016864 vs. TAIR 10
Match: AT4G39080.1 (vacuolar proton ATPase A3 )

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 601/811 (74.11%), Postives = 706/811 (87.05%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSE MQL QLI+P ESAH T+SYLGDLGL QF DLN+ +SPFQRTYA  QIKRCGE
Sbjct: 14  MDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPFQRTYAA-QIKRCGE 73

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           MARK+RFFRDQM++AG+           +DLD++EVKLGELEAEL+EIN NN+KLQR+++
Sbjct: 74  MARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVEINANNDKLQRSYN 133

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQRELEVQQNKEGSIDSPLLLEQEMTTDPMKQVKL 181
           EL+EYKLVLQK GEFF  A   AA QQRE E QQ  E  ++SP LL++E + D  KQVKL
Sbjct: 134 ELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESP-LLQEEKSIDSTKQVKL 193

Query: 182 GYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGER 241
           G+++GLVPREKSM FERILFR+TRGN+++RQ V++  V DP SGEK EKNVFV+F+SGER
Sbjct: 194 GFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKAEKNVFVVFYSGER 253

Query: 242 AKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIGH 301
           AK+KILKICEAFGANRYPF++DLG+Q QMITEVSG+LSELKTTID G   R+ L QTIG 
Sbjct: 254 AKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAGLGQRNILLQTIGD 313

Query: 302 QYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKSQ 361
           ++E WNL V+KEK+IYHTLNMLS+DVTKKCLV EGW PV A +++IQ+A+ +A  DS SQ
Sbjct: 314 KFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFA-SREIQDALQRAAVDSNSQ 373

Query: 362 IEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVMF 421
           + +IF VLRTKE+PPTYFRTNKFTS+ QEIVDAYGVAKYQEANPGV+TI+TFPFLFAVMF
Sbjct: 374 VGSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFTIVTFPFLFAVMF 433

Query: 422 GDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEFF 481
           GDWGHGIC+LLAT+Y I +EKK +S+KLGDI+EM FGGRYVI+MM+LFSIYTGLIYNEFF
Sbjct: 434 GDWGHGICILLATMYLILKEKKLASQKLGDIMEMAFGGRYVILMMSLFSIYTGLIYNEFF 493

Query: 482 SVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKMS 541
           S+PF LF PSAY CRD SC +ATTIGLIKVRDTYPFG+DP WHG+RSELPFLNSLKMKMS
Sbjct: 494 SIPFPLFAPSAYDCRDVSCSEATTIGLIKVRDTYPFGLDPVWHGSRSELPFLNSLKMKMS 553

Query: 542 ILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQA 601
           ILLGV+QMNLGII+SYFNA+FF  ++NIW+QF+PQMIFLNSLFGYLS+LI++KWC+GSQA
Sbjct: 554 ILLGVSQMNLGIIMSYFNARFFKSSVNIWFQFIPQMIFLNSLFGYLSVLIIIKWCTGSQA 613

Query: 602 DLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEERH 661
           DLYH+MIYMFLSP D+LGENQLFP QK LQL+L+  AL++VP MLLPKPF+LKKQHE RH
Sbjct: 614 DLYHVMIYMFLSPMDELGENQLFPHQKTLQLVLLFLALVSVPCMLLPKPFILKKQHEARH 673

Query: 662 QGQSYSLIHSTDDNDETE-HSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASYLRL 721
           QGQ+Y+ +  TD++   E +  GSH HEEF+FSE+FVHQLIHTIEFVLGAVSNTASYLRL
Sbjct: 674 QGQAYAPLDETDESLHVETNGGGSHGHEEFEFSEIFVHQLIHTIEFVLGAVSNTASYLRL 733

Query: 722 WALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLHALR 781
           WALSLAHSELSSVFY+K+LLLAWG+N+ +I  VGV+VFI ATVGVLLVMETLSAFLHALR
Sbjct: 734 WALSLAHSELSSVFYEKVLLLAWGYNNPLILIVGVLVFIFATVGVLLVMETLSAFLHALR 793

Query: 782 LHWVEFQNKFYAGDGFKFSPFSFSSLRDEDE 812
           LHWVEFQNKFY GDG+KF+PF+F    +EDE
Sbjct: 794 LHWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of MS016864 vs. TAIR 10
Match: AT2G28520.1 (vacuolar proton ATPase A1 )

HSP 1 Score: 1018.5 bits (2632), Expect = 3.1e-297
Identity = 503/807 (62.33%), Postives = 637/807 (78.93%), Query Frame = 0

Query: 2   MDLLRSEPMQLAQLIIPNESAHRTISYLGDLGLFQFNDLNASRSPFQRTYATHQIKRCGE 61
           MDL+RSE M L QLIIP ESAHR+I+YLG+LGL QF DLNA +SPFQRT+A +Q+KRCGE
Sbjct: 11  MDLMRSEKMTLVQLIIPVESAHRSITYLGELGLLQFRDLNADKSPFQRTFA-NQVKRCGE 70

Query: 62  MARKLRFFRDQMARAGLSPSTNSLGVPGLDLDNLEVKLGELEAELLEINDNNEKLQRNHS 121
           M+RKLRFF+DQ+ +AGL  S      P + L +LE +L + E E+LE+N N+EKL++ ++
Sbjct: 71  MSRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLERQLADHEHEVLEMNSNSEKLRQTYN 130

Query: 122 ELLEYKLVLQKVGEFFQLAQSIAAAQQREL-EVQQNKEGSIDSPLLLEQEMTTDPMKQVK 181
           ELLE+K+VL+K   F   + + A  ++ EL E   +  G I++  LLEQEM      Q  
Sbjct: 131 ELLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYSNNGFIETASLLEQEMNPGHSNQSG 190

Query: 182 LGYISGLVPREKSMAFERILFRSTRGNMYLRQAVVDGSVTDPVSGEKVEKNVFVIFFSGE 241
           L +ISG++ ++K + FER+LFR+TRGNM   Q   D  + DP + E VEK VFV+FFSGE
Sbjct: 191 LRFISGIINKDKLLKFERMLFRATRGNMLFNQTTSDEEIMDPSTSEMVEKVVFVVFFSGE 250

Query: 242 RAKNKILKICEAFGANRYPFTDDLGKQLQMITEVSGKLSELKTTIDVGKLHRSKLFQTIG 301
           +A+ KILKICEAFGAN YP  +D  KQ Q+  EV  +LS+L+ T+D G  HR+    ++G
Sbjct: 251 QARTKILKICEAFGANCYPVPEDTTKQRQLTREVLSRLSDLEATLDAGTRHRNNALNSVG 310

Query: 302 HQYEQWNLLVKKEKSIYHTLNMLSVDVTKKCLVGEGWCPVLATNQQIQNAMHKATYDSKS 361
           +    W   V++EK++Y TLNML+ DVTKKCLVGEGWCP  A   QI   + +AT+DS S
Sbjct: 311 YSLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEGWCPTFA-KTQIHEVLQRATFDSSS 370

Query: 362 QIEAIFHVLRTKEAPPTYFRTNKFTSSFQEIVDAYGVAKYQEANPGVYTIITFPFLFAVM 421
           Q+  IFHV++  E+PPTYFRTNK T++FQEI+DAYGVA+YQEANP VY+++T+PFLFAVM
Sbjct: 371 QVGVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGVARYQEANPAVYSVVTYPFLFAVM 430

Query: 422 FGDWGHGICLLLATLYFITREKKFSSEKLGDIIEMTFGGRYVIMMMALFSIYTGLIYNEF 481
           FGDWGHG+CLLL  LY + RE+K S++KLG  +EM FGGRYVI++MALFSIY GLIYNEF
Sbjct: 431 FGDWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLFGGRYVILLMALFSIYCGLIYNEF 490

Query: 482 FSVPFELFGPSAYGCRDTSCRDATTIGLIKVRDTYPFGVDPKWHGTRSELPFLNSLKMKM 541
           FSVPF +FG SAY CRDT+C DA T+GLIK RD YPFGVDP W G+R+ELP+LNSLKMKM
Sbjct: 491 FSVPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPFGVDPSWRGSRTELPYLNSLKMKM 550

Query: 542 SILLGVAQMNLGIILSYFNAKFFGENINIWYQFVPQMIFLNSLFGYLSLLILVKWCSGSQ 601
           SILLG+AQMNLG+ILS+FNA+FFG +++I YQF+PQMIFLNSLFGYLSLLI++KWC+GSQ
Sbjct: 551 SILLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQMIFLNSLFGYLSLLIIIKWCTGSQ 610

Query: 602 ADLYHIMIYMFLSPTDDLGENQLFPGQKFLQLMLVLSALIAVPWMLLPKPFLLKKQHEER 661
           ADLYH+MIYMFLSPT++LGEN+LF GQ+ LQ++L+L A IAVPWML PKPF L+K H ER
Sbjct: 611 ADLYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLLAFIAVPWMLFPKPFALRKIHMER 670

Query: 662 HQGQSYSLIHSTD---DNDETEHSHGSHSHEEFDFSEVFVHQLIHTIEFVLGAVSNTASY 721
            QG++Y ++ S++   D +      G H  EEF+FSE+FVHQLIH+IEFVLG+VSNTASY
Sbjct: 671 FQGRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSEIFVHQLIHSIEFVLGSVSNTASY 730

Query: 722 LRLWALSLAHSELSSVFYDKILLLAWGFNSLMIRAVGVVVFICATVGVLLVMETLSAFLH 781
           LRLWALSLAHSELS+VFY+K+LLLAWG+ +++IR +GV VF  AT  +LL+METLSAFLH
Sbjct: 731 LRLWALSLAHSELSTVFYEKVLLLAWGYENILIRLIGVAVFAFATAFILLMMETLSAFLH 790

Query: 782 ALRLHWVEFQNKFYAGDGFKFSPFSFS 805
           ALRLHWVEF  KF+ GDG+KF PFSF+
Sbjct: 791 ALRLHWVEFMGKFFNGDGYKFKPFSFA 815