MS016829 (gene) Bitter gourd (TR) v1

BLAST of MS016829 vs. NCBI nr
Match: XP_022144296.1 (uncharacterized protein LOC111014011 [Momordica charantia])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.7e-300
Identity = 517/523 (98.85%), Postives = 518/523 (99.04%), Query Frame = 0

Query: 102 MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 161
           MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW
Sbjct: 1   MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 60

Query: 162 ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 221
           ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV
Sbjct: 61  ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 120

Query: 222 RAARRAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 281
           RAARRAYDLH DAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT
Sbjct: 121 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 180

Query: 282 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGA 341
           AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFG+
Sbjct: 181 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 240

Query: 342 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 401
           AGLEPGCVAPSYGLAENCTFVSTAWRKGWW PAMPSHRKLLPCGWVGGEDEEIVVAVVNG
Sbjct: 241 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 300

Query: 402 ETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 461
           ETGEVVDD VEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG
Sbjct: 301 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 360

Query: 462 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVAEM 521
           ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQ YSAFLRGGCLAAVKISETVAVVAEM
Sbjct: 361 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 420

Query: 522 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 581
           QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG
Sbjct: 421 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 480

Query: 582 GGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           GGMSVLMAVKFGKPCGGLK FRDLKDSAEGRSFGTCPLLLSLL
Sbjct: 481 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 523

BLAST of MS016829 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.1 bits (2265), Expect = 9.1e-251
Identity = 458/646 (70.90%), Postives = 518/646 (80.19%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKN-Y 120
           VQ IS +L+RQ+RRR+TV++LC  GLDLV+LIYGCQRAGLVSVPI PPDP F  +NKN +
Sbjct: 74  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCF--ENKNCH 133

Query: 121 DLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE- 180
            L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL  V+WISME+L+     E 
Sbjct: 134 HLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEA 193

Query: 181 --------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAA 240
                     D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR A
Sbjct: 194 DQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVA 253

Query: 241 RRAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFR 300
           R+AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+
Sbjct: 254 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFK 313

Query: 301 ATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGL 360
           ATCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F   GL
Sbjct: 314 ATCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGL 373

Query: 361 EPGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCGWVGGEDE-EIVVAV 420
            PGCV+PSYGLAENCTFVSTAW  G      W PAMPS+RKLLPC  +  E   EI V V
Sbjct: 374 NPGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEVVV 433

Query: 421 VNGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGV 480
           VNGETGE+VDDGVEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGV
Sbjct: 434 VNGETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGV 493

Query: 481 IKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVV 540
           IKG DRFLFVIGR SD+IKLN++Q+IHPHYIESIAY  +SA+LRGGCLAA+KIS+T+AVV
Sbjct: 494 IKGTDRFLFVIGRCSDVIKLNNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKISDTIAVV 553

Query: 541 AEMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 600
           AEMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEK
Sbjct: 554 AEMQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEK 613

Query: 601 LAGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           LAGGGMSVLMAVKFG  CG  K+             G  PLLLSLL
Sbjct: 614 LAGGGMSVLMAVKFGTTCGVTKDLE-----------GNRPLLLSLL 645

BLAST of MS016829 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 869.8 bits (2246), Expect = 1.4e-248
Identity = 456/645 (70.70%), Postives = 517/645 (80.16%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 133

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISME+L+     E  
Sbjct: 134 LARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEAD 193

Query: 181 -------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 194 QVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 253

Query: 241 RAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 254 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 313

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F A GL 
Sbjct: 314 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCGWVGGE-DEEIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      W PAMPS+RKLLP   +  E   EI V VV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+DGVEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 493

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY   S++LRGGCLAA+KIS+T+AVVA
Sbjct: 494 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 553

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 554 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 613

Query: 601 AGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           AGGGMSVLMAVKFGK CG  K+    +           PLLLSLL
Sbjct: 614 AGGGMSVLMAVKFGKTCGVTKDLETNR-----------PLLLSLL 645

BLAST of MS016829 vs. NCBI nr
Match: KAG7029389.1 (fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 864.8 bits (2233), Expect = 4.7e-247
Identity = 455/645 (70.54%), Postives = 514/645 (79.69%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISMESL+     E  
Sbjct: 121 LARALSQTKPWAAIAHQAYIASVFRYLSLSPIDGKLALLLRSVQWISMESLRTCTAAEAD 180

Query: 181 -------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR AR
Sbjct: 181 QVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVAR 240

Query: 241 RAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F A GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCGWVGGE-DEEIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      W PAMPS+RKLLP   +  E   EI V VV
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 420

Query: 421 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+DGVEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY   S++LRGGCLAA+KIS+T+AVVA
Sbjct: 481 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 541 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           AGGGMSVLMAVKFGK CG  K+    +           PLLLS L
Sbjct: 601 AGGGMSVLMAVKFGKTCGITKDLETNR-----------PLLLSFL 632

BLAST of MS016829 vs. NCBI nr
Match: KAG6598448.1 (hypothetical protein SDJN03_08226, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 861.3 bits (2224), Expect = 5.2e-246
Identity = 455/645 (70.54%), Postives = 514/645 (79.69%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISMESL+     E  
Sbjct: 121 LARALSQTKPWAAIAHQAYIASVFRYLSLSLIDGKLALLLRSVQWISMESLRTCTAAEAD 180

Query: 181 -------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVR AR
Sbjct: 181 QVVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVAR 240

Query: 241 RAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F A GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCGWVGGE-DEEIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      W PAMPS+RKLLP   +  E   EI V VV
Sbjct: 361 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 420

Query: 421 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+DGVEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY   S++LRGGCLAA+KIS+T+AVVA
Sbjct: 481 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 541 EMQRQDKNDAESLRKICEGIRKAALIE-GIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           AGGGMSVLMAVKFGK CG  K+    +           PLLLS L
Sbjct: 601 AGGGMSVLMAVKFGKTCGVTKDLETNR-----------PLLLSFL 631

BLAST of MS016829 vs. ExPASy Swiss-Prot
Match: Q7TXM1 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.4e-41
Identity = 165/585 (28.21%), Postives = 257/585 (43.93%), Query Frame = 0

Query: 37  WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGL 96
           +  D  G    LT+ Q++    +I+  L       + V +L   GL+ V    G  +AG 
Sbjct: 30  YGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGF 89

Query: 97  VSVPIMPP-----DPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDEN 156
           ++VP+  P     D R +A         VL  +KP A +   + +  V +Y +S   D  
Sbjct: 90  IAVPLSTPQYGIHDDRVSA---------VLQDSKPVAILTTSSVVGDVTKYAAS--HDGQ 149

Query: 157 LATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVV 216
            A ++  V  + ++S   P+++  P +S+     + G        +QYTSG+T  P  V+
Sbjct: 150 PAPVVVEVDLLDLDS---PRQM--PAFSR----QHTGAA-----YLQYTSGSTRTPAGVI 209

Query: 217 VSAGAAAHNVRAARRAY----DLHPDAVIVSWLPQYHDCGLMF-LLLTVTCGATCVLTSP 276
           VS      NV  +   Y       P   +VSWLP YHD GL+  +   +      VL SP
Sbjct: 210 VSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAVLMSP 269

Query: 277 GRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEP 336
             F+  P  W+ L+       +  P FA  L V+R  +      G++L  +  ++  +E 
Sbjct: 270 MSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQD--MAGLDLRDVVGIVSGSER 329

Query: 337 IYRSAVEEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLL---- 396
           I+ + V  F+E F    L P  + PSYGLAE   +V+              + +L     
Sbjct: 330 IHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQA 389

Query: 397 -PCGWVGGEDEEIV---------VAVVNGETGEVVDDGVEGEIWVSSPSNASGYLGNPSV 456
            PCG  G    E++         V +VN ET      GV GEIWV       GY   P  
Sbjct: 390 RPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQ 449

Query: 457 TRETFHSKLRNNERSR---SFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYI 516
           T + F +KL +   +     ++RTGD GVI  +D  LF++GR  D++ ++  +  +P  I
Sbjct: 450 TAQVFDAKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDG-RNHYPDDI 509

Query: 517 ESIAYQTYSAFLRGGCLAAV----KISETVAVVAEMQRADQNDAE---SLKRLCEGIRRA 576
           E+   +     + GG  AA+     I+E +  + E +R      E    L+ +   +  A
Sbjct: 510 EATIQE-----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSA 569

Query: 577 ALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVL 588
                 + V  +VLV  GS+P TTSGKIRRSA  E+    G   L
Sbjct: 570 ISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRL 579

BLAST of MS016829 vs. ExPASy Swiss-Prot
Match: P9WQ42 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD26 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.2e-41
Identity = 164/585 (28.03%), Postives = 257/585 (43.93%), Query Frame = 0

Query: 37  WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGL 96
           +  D  G    LT+ Q++    +I+  L       + V +L   GL+ V    G  +AG 
Sbjct: 30  YGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGF 89

Query: 97  VSVPIMPP-----DPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDEN 156
           ++VP+  P     D R +A         VL  +KP A +   + +  V +Y +S   D  
Sbjct: 90  IAVPLSTPQYGIHDDRVSA---------VLQDSKPVAILTTSSVVGDVTKYAAS--HDGQ 149

Query: 157 LATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVV 216
            A ++  V  + ++S   P+++  P +S+     + G        +QYTSG+T  P  V+
Sbjct: 150 PAPVVVEVDLLDLDS---PRQM--PAFSR----QHTGAA-----YLQYTSGSTRTPAGVI 209

Query: 217 VSAGAAAHNVRAARRAY----DLHPDAVIVSWLPQYHDCGLMF-LLLTVTCGATCVLTSP 276
           VS      NV  +   Y       P   +VSWLP YHD GL+  +   +      +L SP
Sbjct: 210 VSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSP 269

Query: 277 GRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEP 336
             F+  P  W+ L+       +  P FA  L V+R  +      G++L  +  ++  +E 
Sbjct: 270 MSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQD--MAGLDLRDVVGIVSGSER 329

Query: 337 IYRSAVEEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLL---- 396
           I+ + V  F+E F    L P  + PSYGLAE   +V+              + +L     
Sbjct: 330 IHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQA 389

Query: 397 -PCGWVGGEDEEIV---------VAVVNGETGEVVDDGVEGEIWVSSPSNASGYLGNPSV 456
            PCG  G    E++         V +VN ET      GV GEIWV       GY   P  
Sbjct: 390 RPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQ 449

Query: 457 TRETFHSKLRNNERSR---SFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYI 516
           T + F +KL +   +     ++RTGD GVI  +D  LF++GR  D++ ++  +  +P  I
Sbjct: 450 TAQVFDAKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDG-RNHYPDDI 509

Query: 517 ESIAYQTYSAFLRGGCLAAV----KISETVAVVAEMQRADQNDAE---SLKRLCEGIRRA 576
           E+   +     + GG  AA+     I+E +  + E +R      E    L+ +   +  A
Sbjct: 510 EATIQE-----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSA 569

Query: 577 ALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVL 588
                 + V  +VLV  GS+P TTSGKIRRSA  E+    G   L
Sbjct: 570 ISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRL 579

BLAST of MS016829 vs. ExPASy Swiss-Prot
Match: P9WQ43 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.2e-41
Identity = 164/585 (28.03%), Postives = 257/585 (43.93%), Query Frame = 0

Query: 37  WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGL 96
           +  D  G    LT+ Q++    +I+  L       + V +L   GL+ V    G  +AG 
Sbjct: 30  YGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGF 89

Query: 97  VSVPIMPP-----DPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDEN 156
           ++VP+  P     D R +A         VL  +KP A +   + +  V +Y +S   D  
Sbjct: 90  IAVPLSTPQYGIHDDRVSA---------VLQDSKPVAILTTSSVVGDVTKYAAS--HDGQ 149

Query: 157 LATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVV 216
            A ++  V  + ++S   P+++  P +S+     + G        +QYTSG+T  P  V+
Sbjct: 150 PAPVVVEVDLLDLDS---PRQM--PAFSR----QHTGAA-----YLQYTSGSTRTPAGVI 209

Query: 217 VSAGAAAHNVRAARRAY----DLHPDAVIVSWLPQYHDCGLMF-LLLTVTCGATCVLTSP 276
           VS      NV  +   Y       P   +VSWLP YHD GL+  +   +      +L SP
Sbjct: 210 VSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSP 269

Query: 277 GRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEP 336
             F+  P  W+ L+       +  P FA  L V+R  +      G++L  +  ++  +E 
Sbjct: 270 MSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRTSDQD--MAGLDLRDVVGIVSGSER 329

Query: 337 IYRSAVEEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLL---- 396
           I+ + V  F+E F    L P  + PSYGLAE   +V+              + +L     
Sbjct: 330 IHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQA 389

Query: 397 -PCGWVGGEDEEIV---------VAVVNGETGEVVDDGVEGEIWVSSPSNASGYLGNPSV 456
            PCG  G    E++         V +VN ET      GV GEIWV       GY   P  
Sbjct: 390 RPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQ 449

Query: 457 TRETFHSKLRNNERSR---SFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYI 516
           T + F +KL +   +     ++RTGD GVI  +D  LF++GR  D++ ++  +  +P  I
Sbjct: 450 TAQVFDAKLVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDG-RNHYPDDI 509

Query: 517 ESIAYQTYSAFLRGGCLAAV----KISETVAVVAEMQRADQNDAE---SLKRLCEGIRRA 576
           E+   +     + GG  AA+     I+E +  + E +R      E    L+ +   +  A
Sbjct: 510 EATIQE-----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSA 569

Query: 577 ALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVL 588
                 + V  +VLV  GS+P TTSGKIRRSA  E+    G   L
Sbjct: 570 ISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRL 579

BLAST of MS016829 vs. ExPASy Swiss-Prot
Match: Q02278 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD28 PE=3 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 4.2e-41
Identity = 161/587 (27.43%), Postives = 258/587 (43.95%), Query Frame = 0

Query: 33  PAFV---WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIY 92
           PAF    + +D  G    LT+ QL+     ++  L R     + VVI    GL+ V    
Sbjct: 21  PAFTFMDYEQDWDGVAITLTWSQLYRRTLNVARELSRCGSTGDRVVISAPQGLEYVVAFL 80

Query: 93  GCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRD 152
           G  +AG ++VP+  P    T +  +     VLS + P A +   + +  V ++++    +
Sbjct: 81  GALQAGRIAVPLSVPQGGVTDERSD----SVLSDSSPVAILTTSSAVDDVVQHVARRPGE 140

Query: 153 ENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKA 212
              +        I ++ L    +L+ P+   F    Y    P   YL QYTSG+T  P  
Sbjct: 141 SPPSI-------IEVDLL----DLDAPNGYTFKEDEY----PSTAYL-QYTSGSTRTPAG 200

Query: 213 VVVSAGAAAHNVRAARRAYDLH------PDAVIVSWLPQYHDCGLMF-LLLTVTCGATCV 272
           VV+S      N       Y         P++ +VSWLP YHD GL+  +   +  G   V
Sbjct: 201 VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV 260

Query: 273 LTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLIL 332
           LTSP  F+  P  W+HL+ +     +  P FA  L  +R  ++     G +LG++  ++ 
Sbjct: 261 LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDD--MAGRDLGNILTILS 320

Query: 333 INEPIYRSAVEEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMP------ 392
            +E +  + ++ F + F    L+   + PSYGLAE   +V+T+       P  P      
Sbjct: 321 GSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATS------KPGQPPETVDF 380

Query: 393 -------SHRKLLPCGWVGGED-------EEIVVAVVNGETGEVVDDGVEGEIWVSSPSN 452
                   H K  PC   G             +V +V+ +T     DG  GEIWV   + 
Sbjct: 381 DTESLSAGHAK--PCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNV 440

Query: 453 ASGYLGNPSVTRETFHSKL---RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLN 512
           A+GY   P  +  TF  K+           ++RTGD G +   D  +F+IGR  D++ + 
Sbjct: 441 ANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLL-IV 500

Query: 513 SDQQIHPHYIESIAYQTYSAFLRGGCLAAVKI-----SETVAVVAEMQRADQNDAESLKR 572
             +   P  IE+    T     RG C AA+ +     +E +  + E+++   +D +++ R
Sbjct: 501 YGRNHSPDDIEA----TIQEITRGRC-AAISVPGDRSTEKLVAIIELKKRGDSDQDAMAR 560

Query: 573 L---CEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           L      +  A     G+ V  +VLV  GS+P TTSGK+RR A  E+
Sbjct: 561 LGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGACVEQ 569

BLAST of MS016829 vs. ExPASy Swiss-Prot
Match: P9WQ58 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD28 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.2e-41
Identity = 161/587 (27.43%), Postives = 258/587 (43.95%), Query Frame = 0

Query: 33  PAFV---WSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRRRETVVILCWAGLDLVELIY 92
           PAF    + +D  G    LT+ QL+     ++  L R     + VVI    GL+ V    
Sbjct: 21  PAFTFMDYEQDWDGVAITLTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFL 80

Query: 93  GCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRD 152
           G  +AG ++VP+  P    T +  +     VLS + P A +   + +  V ++++    +
Sbjct: 81  GALQAGRIAVPLSVPQGGVTDERSD----SVLSDSSPVAILTTSSAVDDVVQHVARRPGE 140

Query: 153 ENLATLLHNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKA 212
              +        I ++ L    +L+ P+   F    Y    P   YL QYTSG+T  P  
Sbjct: 141 SPPSI-------IEVDLL----DLDAPNGYTFKEDEY----PSTAYL-QYTSGSTRTPAG 200

Query: 213 VVVSAGAAAHNVRAARRAYDLH------PDAVIVSWLPQYHDCGLMF-LLLTVTCGATCV 272
           VV+S      N       Y         P++ +VSWLP YHD GL+  +   +  G   V
Sbjct: 201 VVMSHQNVRVNFEQLMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAV 260

Query: 273 LTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLIL 332
           LTSP  F+  P  W+HL+ +     +  P FA  L  +R  ++     G +LG++  ++ 
Sbjct: 261 LTSPVSFLQRPARWMHLMASDFHAFSAAPNFAFELAARRTTDDD--MAGRDLGNILTILS 320

Query: 333 INEPIYRSAVEEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMP------ 392
            +E +  + ++ F + F    L+   + PSYGLAE   +V+T+       P  P      
Sbjct: 321 GSERVQAATIKRFADRFARFNLQERVIRPSYGLAEATVYVATS------KPGQPPETVDF 380

Query: 393 -------SHRKLLPCGWVGGED-------EEIVVAVVNGETGEVVDDGVEGEIWVSSPSN 452
                   H K  PC   G             +V +V+ +T     DG  GEIWV   + 
Sbjct: 381 DTESLSAGHAK--PCAGGGATSLISYMLPRSPIVRIVDSDTCIECPDGTVGEIWVHGDNV 440

Query: 453 ASGYLGNPSVTRETFHSKL---RNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLN 512
           A+GY   P  +  TF  K+           ++RTGD G +   D  +F+IGR  D++ + 
Sbjct: 441 ANGYWQKPDESERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLL-IV 500

Query: 513 SDQQIHPHYIESIAYQTYSAFLRGGCLAAVKI-----SETVAVVAEMQRADQNDAESLKR 572
             +   P  IE+    T     RG C AA+ +     +E +  + E+++   +D +++ R
Sbjct: 501 YGRNHSPDDIEA----TIQEITRGRC-AAISVPGDRSTEKLVAIIELKKRGDSDQDAMAR 560

Query: 573 L---CEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEK 579
           L      +  A     G+ V  +VLV  GS+P TTSGK+RR A  E+
Sbjct: 561 LGAIKREVTSALSSSHGLSVADLVLVAPGSIPITTSGKVRRGACVEQ 569

BLAST of MS016829 vs. ExPASy TrEMBL
Match: A0A6J1CSZ4 (uncharacterized protein LOC111014011 OS=Momordica charantia OX=3673 GN=LOC111014011 PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.3e-300
Identity = 517/523 (98.85%), Postives = 518/523 (99.04%), Query Frame = 0

Query: 102 MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 161
           MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW
Sbjct: 1   MPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 60

Query: 162 ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 221
           ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV
Sbjct: 61  ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 120

Query: 222 RAARRAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 281
           RAARRAYDLH DAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT
Sbjct: 121 RAARRAYDLHTDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVT 180

Query: 282 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGA 341
           AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFG+
Sbjct: 181 AFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGS 240

Query: 342 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 401
           AGLEPGCVAPSYGLAENCTFVSTAWRKGWW PAMPSHRKLLPCGWVGGEDEEIVVAVVNG
Sbjct: 241 AGLEPGCVAPSYGLAENCTFVSTAWRKGWWFPAMPSHRKLLPCGWVGGEDEEIVVAVVNG 300

Query: 402 ETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 461
           ETGEVVDD VEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG
Sbjct: 301 ETGEVVDDDVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKG 360

Query: 462 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVAEM 521
           ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQ YSAFLRGGCLAAVKISETVAVVAEM
Sbjct: 361 ADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQNYSAFLRGGCLAAVKISETVAVVAEM 420

Query: 522 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 581
           QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG
Sbjct: 421 QRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAG 480

Query: 582 GGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           GGMSVLMAVKFGKPCGGLK FRDLKDSAEGRSFGTCPLLLSLL
Sbjct: 481 GGMSVLMAVKFGKPCGGLKAFRDLKDSAEGRSFGTCPLLLSLL 523

BLAST of MS016829 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 7.0e-249
Identity = 456/645 (70.70%), Postives = 517/645 (80.16%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS  L+RQ+RRR+TVVILC  GLDLV+LIYGCQRAGLVSVPI PPDPRF  +N ++ 
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHH- 133

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
           L R LSQTKP+AAIAHQAYIA+VFRYLS +  D  LA LL +V+WISME+L+     E  
Sbjct: 134 LARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEAD 193

Query: 181 -------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 194 QVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 253

Query: 241 RAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 254 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 313

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLE 360
           TCTPVP F LPLV+KR KE     GG++L SLRNLILINEP+YRS VEEFV++F A GL 
Sbjct: 314 TCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCGWVGGE-DEEIVVAVV 420
           PGCV+PSYGLAENCTFVSTAW  G      W PAMPS+RKLLP   +  E   EI V VV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+V+DGVEGEIW+SSPSNASGYLG+PS+TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKL-SNKSSHKFVRTGDRGVI 493

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVA 540
           KG DRFLFVIGR SD+I L ++Q+IHPHYIESIAY   S++LRGGCLAA+KIS+T+AVVA
Sbjct: 494 KGTDRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVA 553

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAESL+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R AAKEKL
Sbjct: 554 EMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKL 613

Query: 601 AGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           AGGGMSVLMAVKFGK CG  K+    +           PLLLSLL
Sbjct: 614 AGGGMSVLMAVKFGKTCGVTKDLETNR-----------PLLLSLL 645

BLAST of MS016829 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 2.0e-243
Identity = 446/645 (69.15%), Postives = 510/645 (79.07%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGA----GAGSVLTYRQLHDS 60
           M +ENFDP FPDQPVVDLYLPVWA+LPAFRSKPAF+WSEDG       GS LTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQ IS +L+RQ+RRR+TV++LC  GLDLV+LIYGCQRAGLVSVPI PPDP F  +N ++ 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHH- 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKPKELE-- 180
             R LSQTKP+A IAH+ YI +VFRYLSS+Q D  LA LL +V+WISME+L+     E  
Sbjct: 121 FARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEAD 180

Query: 181 -------EPDYSKFCH--SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAAR 240
                    D   F H  SSY GCKPEEPYLIQYTSGATG PK VVV+AGAAAHNVRAAR
Sbjct: 181 QVVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAAR 240

Query: 241 RAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRA 300
           +AYDL+P+ VIVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+T F+A
Sbjct: 241 KAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKA 300

Query: 301 TCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLE 360
           TCTPVP F LPLV+KR KE     G ++L  LRNLILINEP+YRS VEEFV++F A GL 
Sbjct: 301 TCTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVAPSYGLAENCTFVSTAWRKG-----WWLPAMPSHRKLLPCG-WVGGEDEEIVVAVV 420
           PGCV+PSYGLAEN TFVSTAW  G        PAMPS+RKLLPC     G   EI V VV
Sbjct: 361 PGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVV 420

Query: 421 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 480
           NGETGE+VDDGVEGEIW+SSPSNASGYLG+P +TR+TFH KL +N+ S  FVRTGDRGVI
Sbjct: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKL-SNKSSHKFVRTGDRGVI 480

Query: 481 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVA 540
           KGADRFLFVIGR SD+I L ++Q+IHPHYIESIAY  +SA+LRGGCLAA+K+S+T+AVVA
Sbjct: 481 KGADRFLFVIGRCSDVITLTNNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVA 540

Query: 541 EMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKL 600
           EMQR D+NDAE+L+++CEGIR+AALIE GIE+GLVVLVKRG+VPKTTSGK++R  AKEKL
Sbjct: 541 EMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKL 600

Query: 601 AGGGMSVLMAVKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
           AGGGMSVLMAVKFG  CG  K+             G  PLLLSLL
Sbjct: 601 AGGGMSVLMAVKFGTICGVTKDLD-----------GNRPLLLSLL 632

BLAST of MS016829 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 7.3e-238
Identity = 441/635 (69.45%), Postives = 513/635 (80.79%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAG----SVLTYRQLHDS 60
           ML+ENFDP FP+QPVVD YLPVWA+LPAF+SKPAF+WSEDG        S LTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYD 120
           VQLI+ +LLRQLRRR+TVV+LC AGLD V+LIYGCQRAGLVSVPI PPD  F+     + 
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDV-FSENENCHH 120

Query: 121 LTRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLKKP-KELE- 180
           L R LSQTKP+AAIAHQ+YI  VFRY+SS+  D+ LA LL  V+WISMESLK+P KE E 
Sbjct: 121 LARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESEL 180

Query: 181 --EPDYSKFCH-SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLH 240
                 S F H SSY GC P++PYLIQYTSGAT   KAVV++AGAAAHNVRAAR+AYDL+
Sbjct: 181 NQHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLN 240

Query: 241 PDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVP 300
           P+ +IVSWLPQYHDCGLMFLLLTV  GATCVLTSP  FV  P+TWLHL+TAF+ATCTPVP
Sbjct: 241 PNDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVP 300

Query: 301 CFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLEPGCVAP 360
            F LPLV+KR   + G AG ++L SLRNLILINEPIYRS VEEFVE+F   GL+PGCV+P
Sbjct: 301 SFTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSP 360

Query: 361 SYGLAENCTFVSTAWRKGWWLPAMPSHRKLLPCGWV-GGEDEEIVVAVVNGETGEVVDDG 420
           SYGLAENCTFVSTAW  G   PAMPS+R+LLP G +  G  +EI V VVN ETGEVV+DG
Sbjct: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420

Query: 421 VEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIG 480
           VEGEIWVSSPSNA GYLG+PSVT ETFHSKL+ N+ S +FVRTGDRGVIKG+DRFLFVIG
Sbjct: 421 VEGEIWVSSPSNALGYLGHPSVTEETFHSKLK-NKSSLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RSSDIIKL-NSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVAEMQRADQNDA 540
           R SD+IKL N++QQIHPHYIES +Y  +SA+LRGGC+AAVK+S T+A+VAEMQR D++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 ESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVLMA 600
           E L+++CE IR+A LIE GIE+GLVVLVKRG+V KTTSGK++R   KEKLAGGG+ VLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VKFGKPCGGLKEFRDLKDSAEGRSFGTCPLLLSLL 625
            +FGK C       DLK   E   F T P+L+S+L
Sbjct: 601 FEFGKNC------VDLKRKGE---FETRPVLISML 623

BLAST of MS016829 vs. ExPASy TrEMBL
Match: A0A6P5TVV7 (uncharacterized protein LOC110771876 OS=Prunus avium OX=42229 GN=LOC110771876 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 7.9e-200
Identity = 370/621 (59.58%), Postives = 467/621 (75.20%), Query Frame = 0

Query: 1   MLFENFDPYFPDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAG--AGSVLTYRQLHDSVQ 60
           M +EN+DP +PDQPVVDLYL VWANLPAFRSKPAFVW ED A   A S LTY QL+DSVQ
Sbjct: 19  MNYENYDPSYPDQPVVDLYLQVWANLPAFRSKPAFVWVEDSAAIDAKSTLTYSQLNDSVQ 78

Query: 61  LISLRLLRQLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYDLT 120
            I+ +LL  L+R +TVVILC  GL+LVE I+GCQRAGL+SVPI PPDP FT QN ++ L 
Sbjct: 79  CIASQLLIPLQRGDTVVILCSPGLELVETIFGCQRAGLLSVPISPPDPSFTNQNFHH-LI 138

Query: 121 RVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKWISMESLK-KPKELEEPD 180
           R LSQTKPKAAIAH  YI ++  Y+SS+  ++ L  +L ++ WIS   +K K ++L+   
Sbjct: 139 RALSQTKPKAAIAHPTYITSINHYISSSSSNKKLVHMLQSLHWISTVDIKAKDRKLQHSQ 198

Query: 181 YS-KFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHPDAVI 240
              KF  S Y+GC+ E+ YL+QYTSGATG PK V+V+AG+AAHNVR AR+AYDLHP++VI
Sbjct: 199 LGPKF--SPYKGCRAEDVYLVQYTSGATGIPKPVLVTAGSAAHNVRTARKAYDLHPNSVI 258

Query: 241 VSWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALP 300
           VSWLPQYHDCGLMFLLLT+  GAT VLTSP  FV  P  WL L+T F+ATCTPVP FALP
Sbjct: 259 VSWLPQYHDCGLMFLLLTIVSGATSVLTSPAAFVNRPRLWLELITQFKATCTPVPSFALP 318

Query: 301 LVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLEPGCVAPSYGLA 360
           LVVKR   + G +  ++L SL+NLI+INEPIYR AVE+FV +F   GL P  ++PSYGLA
Sbjct: 319 LVVKRGGIDKGTS-PIHLWSLQNLIIINEPIYRDAVEQFVNVFRTFGLNPSSISPSYGLA 378

Query: 361 ENCTFVSTAWRK--GWWLPAMPSHRKLLPCGWVGGEDEEIV---VAVVNGETGEVVDDGV 420
           ENCTFVSTAWR+     +P MPS+ KLLP   +G  D+E+V   + VVN ET E V+DG+
Sbjct: 379 ENCTFVSTAWRRPCSNHVPNMPSYNKLLPSARLGQNDDELVDMDIIVVNDETHEAVEDGI 438

Query: 421 EGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGR 480
           EGEIWVSSPSNASGYLG+P++TRE ++ +L+ N+ SRSF+RTGDRGV+KG +R+LFV+GR
Sbjct: 439 EGEIWVSSPSNASGYLGHPTMTREVYYGRLK-NKVSRSFLRTGDRGVVKGDERYLFVMGR 498

Query: 481 SSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAAVKISETVAVVAEMQRADQNDAES 540
            +D+IK  + ++IHPHYIE+ AY +   FLR GC AA +IS TV VV EMQR ++ +   
Sbjct: 499 CADVIKRQNHEEIHPHYIEAAAYNSSPWFLRAGCSAAFEISNTVVVVVEMQR-NEKEIGV 558

Query: 541 LKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRRSAAKEKLAGGGMSVLMAVK 600
           L+++CEG+R+    E  +EVG+VVLV+ GSVPKTTSGKI+R A K+KL GG MSVLM ++
Sbjct: 559 LRKICEGVRKGVAEEERVEVGMVVLVRSGSVPKTTSGKIQRWATKDKLIGGKMSVLMEMR 618

Query: 601 FGKPCGGLKEFRDLKDSAEGR 613
           FG        F  +   +EG+
Sbjct: 619 FGDYGDSFSSFGIIIGESEGK 633

BLAST of MS016829 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.8e-10
Identity = 76/307 (24.76%), Postives = 127/307 (41.37%), Query Frame = 0

Query: 192 EEPYLIQYTSGATGPPKAVVVSAG-AAAHNVRAARRAYDLHPDAVIVSWLPQYHDCGLM- 251
           ++  ++ Y+SG TG  K V  S G   AH  R     ++  P    +  +P +H  GL+ 
Sbjct: 199 DDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFE-QPQQTFICTVPLFHTFGLLN 258

Query: 252 FLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVA 311
           F+L T+  G T V+  P   +G  M     V  +RAT         P++V    +   + 
Sbjct: 259 FVLATLALGTTVVIL-PRFDLGEMMA---AVEKYRATTL---ILVPPVLVTMINKADQIM 318

Query: 312 GGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLEPG-CVAPSYGLAENCTFVSTAWRK 371
              ++  LR +     P+ +   + F++ +    +  G  +  S G   +   V  + R 
Sbjct: 319 KKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTESNGAGASIESVEESRRY 378

Query: 372 GWWLPAMPSHRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDGVEGEIWVSSPSNASGYLG 431
           G           LL CG        +   +V+  TG+V+     GE+W+  PS A GY  
Sbjct: 379 G--------AVGLLSCG--------VEARIVDPNTGQVMGLNQTGELWLKGPSIAKGY-- 438

Query: 432 NPSVTRETFHSKLRNNER---SRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIH 491
                        RN E    S  +++TGD   I   D FLF++ R  ++IK     Q+ 
Sbjct: 439 ------------FRNEEEIITSEGWLKTGDLCYIDN-DGFLFIVDRLKELIKYKG-YQVP 465

Query: 492 PHYIESI 493
           P  +E++
Sbjct: 499 PAELEAL 465

BLAST of MS016829 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 3.0e-10
Identity = 121/535 (22.62%), Postives = 216/535 (40.37%), Query Frame = 0

Query: 48  LTYRQLHDSVQLISLRLLRQ--LRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPD 107
           LT+ +LHD ++  + RL+    ++  + V +     ++ V +     RA   + P+   +
Sbjct: 31  LTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPL---N 90

Query: 108 PRFTAQNKNYDL----TRVLSQTKPKAAIAHQAYIAAVFRYLSSTQRDENLATLLHNVKW 167
             +TA+   + L    +++L  +K   A A +A       ++++T  D     +L     
Sbjct: 91  AAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKLKISHVTATLLDAGSDLVL----- 150

Query: 168 ISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNV 227
               S+     + +       H       P++  L  +TSG T  PK V ++    A +V
Sbjct: 151 ----SVADSDSVVDSATELVNH-------PDDGALFLHTSGTTSRPKGVPLTQLNLASSV 210

Query: 228 RAARRAYDLHPDAVIVSWLPQYHDCGLMF-LLLTVTCGATCVLTSPGRFVGSPMTWLHLV 287
           +  +  Y L      V  LP +H  GL+  LL ++  GA   L + GRF  S  T+   +
Sbjct: 211 KNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRF--SATTFWPDM 270

Query: 288 TAFRAT-CTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMF 347
             + AT  T VP     +++ R   +P       L  +R+      P+  S +E   E F
Sbjct: 271 KKYNATWYTAVPTIH-QIILDRHASHPETE-YPKLRFIRSCSASLAPVILSRLE---EAF 330

Query: 348 GAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLLPCGWVGGEDEEIVVAVV 407
           GA  LE      +Y + E    +S+       LP    H+       VG E     +A++
Sbjct: 331 GAPVLE------AYAMTEATHLMSSN-----PLPEEGPHKPGSVGKPVGQE-----MAIL 390

Query: 408 NGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVI 467
           N E GE+ +   +GE+ +  P+   GY  NP   +  F            +  TGD G  
Sbjct: 391 N-EKGEIQEPNNKGEVCIRGPNVTKGYKNNPEANKAGFEF---------GWFHTGDIGYF 450

Query: 468 KGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQTYSAFLRGGCLAA--VKISETVAV 527
              D +L ++GR  ++I     ++I P  ++++   T+    +G        K  E +  
Sbjct: 451 -DTDGYLHLVGRIKELIN-RGGEKISPIEVDAVLL-THPDVSQGVAFGVPDEKYGEEINC 503

Query: 528 VAEMQRADQNDAESLKRLCEGIRRAALIEGGIEVGLVVLVKRGSVPKTTSGKIRR 573
               +       E +K  C+    A  +   + +         ++PKT SGKI+R
Sbjct: 511 AVIPREGTTVTEEDIKAFCKKNLAAFKVPKRVFI-------TDNLPKTASGKIQR 503

BLAST of MS016829 vs. TAIR 10
Match: AT1G20490.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 5.1e-10
Identity = 89/406 (21.92%), Postives = 160/406 (39.41%), Query Frame = 0

Query: 42  AGAGSVLTYRQLHDSVQLISLRLLRQ--LRRRETVVILCWAGLDLVELIYGCQRAGLVSV 101
           A  G  LT+  L   V  ++  L  +  +RR + V+IL    + +  +       G V  
Sbjct: 58  ASTGHRLTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVT 117

Query: 102 PIMPPDPRFTAQNKNYDLTRVLSQTKPKAAIAHQAY---IAAVFRYLSSTQRDENLATLL 161
                       N + ++++ ++Q+ P            +AA    + + + DE    L 
Sbjct: 118 TA-------NTLNTSGEISKQIAQSNPTLVFTTSQLAPKLAAAISVVLTDEEDEKRVELT 177

Query: 162 HNVKWISMESLKKPKELEEPDYSKFCHSSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGA 221
             V+ + + S    KE          +        ++  ++ Y+SG TG  K V+    +
Sbjct: 178 SGVRVVGILSEMMKKETSGQRVRDRVNQ-------DDTAMMLYSSGTTGTSKGVI----S 237

Query: 222 AAHNVRAARRAY---DLHPDAVIVSWLPQYHDCGLM-FLLLTVTCGATCVLTSPGRFVGS 281
           +  N+ A    Y       D + V  +P +H  GL+ F + +V  G+T V+    RF   
Sbjct: 238 SHRNLTAYVAKYIDDKWKRDEIFVCTVPMFHSFGLLAFAMGSVASGSTVVILR--RFGLD 297

Query: 282 PMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAV 341
            M  +  V  ++AT   +   A P++V        +    +L SLR +     P+ +  +
Sbjct: 298 DM--MQAVEKYKAT---ILSLAPPVLVAMINGADQLKAKYDLTSLRKVRCGGAPLSKEVM 357

Query: 342 EEFVEMFGAAGLEPGCVAPSYGLAENCTFVSTAWRKGWWLPAMPSHRKLLPCGWVGGEDE 401
           + F+E +    +  G     Y L E+    ++            S  + L  G VG    
Sbjct: 358 DSFLEKYPTVNIFQG-----YALTESHGSGAST----------ESVEESLKYGAVGLLSS 417

Query: 402 EIVVAVVNGETGEVVDDGVEGEIWVSSPSNASGYLGNPSVTRETFH 439
            I   +V+ +TG V+     GE+W+  PS + GY GN   T ET +
Sbjct: 418 GIEARIVDPDTGRVMGVNQPGELWLKGPSISKGYFGNEEATNETIN 423

BLAST of MS016829 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 8.8e-10
Identity = 118/495 (23.84%), Postives = 186/495 (37.58%), Query Frame = 0

Query: 11  PDQPVVDLYLPVWANLPAFRSKPAFVWSEDGAGAGSVLTYRQLHDSVQLISLRLLRQLRR 70
           P  P   L   ++ N  ++ SK A   S+     G  LT+ QL  +V  ++    R   R
Sbjct: 22  PKDPNTSLVSFLFRNSSSYPSKLAIADSD----TGDSLTFSQLKSAVARLAHGFHRLGIR 81

Query: 71  RETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPRFTAQNKNYDLTRVLSQTK---PK 130
           +  VV++               +  L  + +      FT  N  Y +  V  Q K   PK
Sbjct: 82  KNDVVLI---------FAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPK 141

Query: 131 AAIAHQAYIAAVFRY----LSSTQRDENLATLLHNVKWISMESLKKPKELEEPDYSKFCH 190
             I+       +  +    +    +D        N K +S +++    EL EP       
Sbjct: 142 IIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNV---MELSEP----VSE 201

Query: 191 SSYRGCKPEEPYLIQYTSGATGPPKAVVVSAGAAAHNVRAARRAYDLHPD------AVIV 250
             +   K  +   + Y+SG TG  K V ++ G    N  AA     +  D       V +
Sbjct: 202 YPFVEIKQSDTAALLYSSGTTGTSKGVELTHG----NFIAASLMVTMDQDLMGEYHGVFL 261

Query: 251 SWLPQYHDCGLMFLLLTVTCGATCVLTSPGRFVGSPMTWLHLVTAFRATCTPVPCFALPL 310
            +LP +H  GL  +  +        L S  RF    +  L  +  FR T   V     P+
Sbjct: 262 CFLPMFHVFGLAVITYSQLQRGN-ALVSMARFELELV--LKNIEKFRVTHLWV---VPPV 321

Query: 311 VVKRAKENPGVAGGVNLGSLRNLILINEPIYRSAVEEFVEMFGAAGLEPGCVAPSYGLAE 370
            +  +K++  +    +L SL+ +     P+ +  +EE         L  G     YG+ E
Sbjct: 322 FLALSKQS--IVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQG-----YGMTE 381

Query: 371 NCTFVSTAWRKGWWLPAMPSHRKLLPCGWVGGEDEEIVVAVVNGETGEVVDDGVEGEIWV 430
            C  VS         P +         G  G     +   +V+ ETG+      +GEIWV
Sbjct: 382 TCGIVSVE------DPRLGKRNS----GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWV 441

Query: 431 SSPSNASGYLGNPSVTRETFHSKLRNNERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIK 490
             P+   GYL NP  T+ET   K        S+V TGD G     D  L+V+ R  ++IK
Sbjct: 442 RGPNMMKGYLNNPQATKETIDKK--------SWVHTGDLGYF-NEDGNLYVVDRIKELIK 459

Query: 491 LNSDQQIHPHYIESI 493
                Q+ P  +E +
Sbjct: 502 YKG-FQVAPAELEGL 459

BLAST of MS016829 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 51.6 bits (122), Expect = 2.6e-06
Identity = 112/472 (23.73%), Postives = 184/472 (38.98%), Query Frame = 0

Query: 49  TYRQLHDSVQLISLRLLR-QLRRRETVVILCWAGLDLVELIYGCQRAGLVSVPIMPPDPR 108
           T+ Q +D    ++  L+   + + + V ++      + E+ +    AG V  PI   + R
Sbjct: 41  TWPQTYDRCCRLAASLISLNIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPI---NTR 100

Query: 109 FTAQNKNYDLTRVLSQTKPKAAIAHQAY---IAAVFRYLSSTQRDENLATLLHNVKWISM 168
             A +    +  +L   KPK    ++++      + + LSS   D NL     N+  I +
Sbjct: 101 LDATS----IAAILRHAKPKILFIYRSFEPLAREILQLLSS--EDSNL-----NLPVIFI 160

Query: 169 ESLKKPKEL--EEPDYS---------------KFCHSSYRGCKPEEPYLIQYTSGATGPP 228
             +  PK +  EE DY                 FC          +P  + YTSG T  P
Sbjct: 161 HEIDFPKRVSSEESDYECLIQRGEPTPLLLARMFCIQD-----EHDPISLNYTSGTTADP 220

Query: 229 KAVVVSAGAAAHNVRAARRAYDLHPDAVIVSWLPQYHDCGLMFLLLTVTCGATCVLTSPG 288
           K VV+S   A  +  +A   +++    V +  LP +H  G  F   T   G T V     
Sbjct: 221 KGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCNGWTFTWGTAARGGTSVCM--- 280

Query: 289 RFVGSPMTWLHLVTAFRATCTPVPCFALPLVVKRAKENPGVAGGVNLGSLRNLILINEPI 348
           R V +P  + ++          VP     L+   + +    +G V+       +L     
Sbjct: 281 RHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRSGPVH-------VLTGGSP 340

Query: 349 YRSAVEEFVEMFGAAGLEPGCVAPSYGLAE-NCTFVSTAWRKGWWLPAMPSHRKLLPCGW 408
             +A+ + V+  G        V  +YGL E     +   W+  W    +P ++++     
Sbjct: 341 PPAALVKKVQRLGFQ------VMHAYGLTEATGPVLFCEWQDEW--NRLPENQQMELKAR 400

Query: 409 VGGEDEEIV-VAVVNGETGEVV--DDGVEGEIWVSSPSNASGYLGNPSVTRETFHSKLRN 468
            G     +  V V N ET E V  D    GEI +   S   GYL NP  T E F      
Sbjct: 401 QGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAF------ 460

Query: 469 NERSRSFVRTGDRGVIKGADRFLFVIGRSSDIIKLNSDQQIHPHYIESIAYQ 496
                 ++ +GD GVI   D  + +  RS DII ++  + I    +E+I Y+
Sbjct: 461 ---KHGWLNSGDVGVIH-PDGHVEIKDRSKDII-ISGGENISSVEVENIIYK 464