MS016774 (gene) Bitter gourd (TR) v1

BLAST of MS016774 vs. NCBI nr
Match: XP_022157315.1 (EP1-like glycoprotein 4, partial [Momordica charantia])

HSP 1 Score: 782.3 bits (2019), Expect = 2.3e-222
Identity = 383/388 (98.71%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 60
           PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP
Sbjct: 52  PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 111

Query: 61  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 120
           PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA
Sbjct: 112 PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 171

Query: 121 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 180
           GHVGWRTGTSGQGVEKLRILRTGNLALVDALE IKWQSFNFPTDVMLLGQSLNANTHLTS
Sbjct: 172 GHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTS 231

Query: 181 FPT--NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 240
           FPT  NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL
Sbjct: 232 FPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 291

Query: 241 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 300
           DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL
Sbjct: 292 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 351

Query: 301 ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 360
           ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILR APKR
Sbjct: 352 ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILRGAPKR 411

Query: 361 VNVSKEECGSWCLEDCKCAAALHYSGGG 387
           VNVSKEECG WCLEDCKCAAALHYSGGG
Sbjct: 412 VNVSKEECGIWCLEDCKCAAALHYSGGG 439

BLAST of MS016774 vs. NCBI nr
Match: XP_022971607.1 (PAN domain-containing protein At5g03700 [Cucurbita maxima])

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-203
Identity = 364/479 (75.99%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL    CRTDI  G+ VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVASAAQICF-VLLLLNLDRTICRTDIVPGHDVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+DQA KIA IPS + QPLRF+ALGN +GNLGLY YSPQKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDQAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSPQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEMGN----SKCGGEEINSGGFCGGIEAEMIELEGISSILREAP 362
           KPYGICTFSNSCSCI  ++ N    SKC  E   SG FCGGIE EM+ELEGISSILR+AP
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDSSKCSDE--ISGKFCGGIEGEMVELEGISSILRDAP 360

Query: 363 KRVNVSKEECGSWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMGL 422
            RVN+SK ECG+WCLEDCKCAAALHYSGGG    GGG ECY YR+VMGVK+I  E+ MG 
Sbjct: 361 NRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRLVMGVKEI--EKGMGF 420

Query: 423 SYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDT 472
           SYMVKVPKG+ L R KS LKKW+L VVGVVD  +I+AV GGLGYYFI RRR NL+  DT
Sbjct: 421 SYMVKVPKGTALERRKSGLKKWVLAVVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRDT 468

BLAST of MS016774 vs. NCBI nr
Match: KAG6596316.1 (putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027869.1 putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 718.8 bits (1854), Expect = 3.1e-203
Identity = 363/482 (75.31%), Postives = 404/482 (83.82%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVGSAAQICF-VLLLLNLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+D+A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDEAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREA 362
           KPYGICTFSNSCSCI  ++      NSKC  E   SG FCGGIE EM+ELEGISSILR+A
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDDNSKCSDE--ISGKFCGGIEGEMVELEGISSILRDA 360

Query: 363 PKRVNVSKEECGSWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMG 422
           P RVN+SK ECG+WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+I  E+ MG
Sbjct: 361 PNRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEI--EKGMG 420

Query: 423 LSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDT 474
            SYMVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT
Sbjct: 421 FSYMVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRDT 471

BLAST of MS016774 vs. NCBI nr
Match: XP_022933279.1 (EP1-like glycoprotein 4 [Cucurbita moschata])

HSP 1 Score: 718.4 bits (1853), Expect = 4.1e-203
Identity = 363/482 (75.31%), Postives = 404/482 (83.82%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVGSAAQICF-VLLLLNLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+D+A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDEAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREA 362
           KPYGICTFSNSCSCI  ++      NSKC  E   SG FCGGIE EM+ELEGISSILR+A
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDDNSKCSDE--ISGKFCGGIEGEMVELEGISSILRDA 360

Query: 363 PKRVNVSKEECGSWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMG 422
           P RVN+SK ECG+WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+I  E+ MG
Sbjct: 361 PNRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEI--EKGMG 420

Query: 423 LSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDT 474
            SYMVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT
Sbjct: 421 FSYMVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILGDT 471

BLAST of MS016774 vs. NCBI nr
Match: XP_023540139.1 (EP1-like glycoprotein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706.4 bits (1822), Expect = 1.6e-199
Identity = 356/483 (73.71%), Postives = 400/483 (82.82%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL    CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVGSAAQICF-VLLLLNLDRTICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDA +G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDASDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+D+A KIA IPS +  PLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDEAMKIAAIPSGTAHPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREA 362
           KPYGICTFSNSCSCI  ++      NSKC  +   SG FCGG E EM+ELEGISSILR+A
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDDNSKCSDQ--ISGKFCGGTEGEMVELEGISSILRDA 360

Query: 363 PKRVNVSKEECGSWCLEDCKCAAALHYS-----GGGGGGGECYAYRVVMGVKQIEREREM 422
           P RVN+SK ECG+WCLEDCKCAAALHYS     GGGGGG ECY YRVVMGVK+I  E+ M
Sbjct: 361 PNRVNLSKRECGNWCLEDCKCAAALHYSGGDDGGGGGGGEECYLYRVVMGVKEI--EKGM 420

Query: 423 GLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTD 474
           G +YMVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR +L+  D
Sbjct: 421 GFNYMVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKSLILRD 472

BLAST of MS016774 vs. ExPASy Swiss-Prot
Match: Q9ZVA5 (EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 5.3e-20
Identity = 87/318 (27.36%), Postives = 138/318 (43.40%), Query Frame = 0

Query: 89  VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 148
           VW +   S     E   L    DG+L L    G V W+T T+ +GV  ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVGIKILENGNMVIY 149

Query: 149 DALEGIKWQSFNFPTDVMLLGQS--LNANTHLTSFPTNANANATSFYSFEIEPQRLALYI 208
           D+     WQSF+ PTD +L+GQS  LN    L S   + + NA   YS  +E ++L LY 
Sbjct: 150 DSNGKFVWQSFDSPTDTLLVGQSLKLNGQNKLVS-RLSPSVNANGPYSLVMEAKKLVLYY 209

Query: 209 NSNSAKSKYSYWEFNPPNNI-NLSFIT------------LNTQGLDIFSDQAKKIATIPS 268
            +N       Y+E+     I  L  +T            L+ +G+D  S     ++T  S
Sbjct: 210 TTNKTPKPIGYYEYEFFTKIAQLQSMTFQAVEDADTTWGLHMEGVD--SGSQFNVSTFLS 269

Query: 269 QSEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRAL-------KTTCDLPLACKP 328
           + +    L FL L  + GN+ ++SYS       ++ ++ A           C +P  C  
Sbjct: 270 RPKHNATLSFLRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDGNDECRIPEHCLG 329

Query: 329 YGICTFSNSCSCIRVEMG----NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 377
           +G+C     C+    ++G    +  C   +I S   C        ++EG  S + +    
Sbjct: 330 FGLCK-KGQCNACPSDIGLLGWDETC---KIPSLASCDPKTFHYFKIEGADSFMTKYNGG 389

BLAST of MS016774 vs. ExPASy Swiss-Prot
Match: Q9ZVA2 (EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.6e-19
Identity = 86/319 (26.96%), Postives = 128/319 (40.13%), Query Frame = 0

Query: 102 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNF 161
           E   L L  +G+L L    G V W+T T+ +GV   +IL  GN+ L D      WQSF+ 
Sbjct: 105 ENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDH 164

Query: 162 PTDVMLLGQSLNAN--THLTSFPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWE 221
           PTD +L GQSL  N    L S  +++N  +   YS  ++ + L +Y+N       Y  W 
Sbjct: 165 PTDTLLTGQSLKVNGVNKLVSRTSDSN-GSDGPYSMVLDKKGLTMYVNKTGTPLVYGGWP 224

Query: 222 FNPPNNINLSFITLNTQGL------DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGL 281
            +         +T     L      ++  + A + AT P  + + L+   +G+  G L L
Sbjct: 225 DHDFRGTVTFAVTREFDNLTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTLNL 284

Query: 282 ----------------------YSYSPQKGI--FEASFRALKT----TCDLPLACKPYGI 341
                                 YSY P      +E SF    T     C LP  C  YG 
Sbjct: 285 NKINYNGTISYLRLGSDGSLKAYSYFPAATYLKWEESFSFFSTYFVRQCGLPSFCGDYGY 344

Query: 342 CT--FSNSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN-- 377
           C     N+C   +  +G + KC   +     FC G++ + +    I  +       VN  
Sbjct: 345 CDRGMCNACPTPKGLLGWSDKCAPPKTTQ--FCSGVKGKTVNYYKIVGVEHFTGPYVNDG 404

BLAST of MS016774 vs. ExPASy Swiss-Prot
Match: Q9ZVA4 (EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.4e-19
Identity = 81/316 (25.63%), Postives = 137/316 (43.35%), Query Frame = 0

Query: 89  VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 148
           VW +   S     E   L    DG+L L    G + W+T T+ +G   ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRLVWQTNTANKGAVGIKILENGNMVIY 149

Query: 149 DALEGIKWQSFNFPTDVMLLGQS--LNANTHLTSFPTNANANATSFYSFEIEPQRLALYI 208
           D+     WQSF+ PTD +L+GQS  LN  T L S   + + N    YS  +E ++L LY 
Sbjct: 150 DSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKLVS-RLSPSVNTNGPYSLVMEAKKLVLYY 209

Query: 209 NSNSAKSKYSYWEFNPPNNINLSFITLNTQGLD------------IFSDQAKKIATIPSQ 268
            +N      +Y+E+     I   F ++  Q ++            + S     ++T  S+
Sbjct: 210 TTNKTPKPIAYFEYEFFTKIT-QFQSMTFQAVEDSDTTWGLVMEGVDSGSKFNVSTFLSR 269

Query: 269 SEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRALKTT-------CDLPLACKPY 328
            +    L F+ L  + GN+ ++SYS       ++ ++ A           C +P  C  +
Sbjct: 270 PKHNATLSFIRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNADTDGNDECRIPEHCLGF 329

Query: 329 GICTFS--NSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 377
           G+C     N+C   +  +G +  C    + S   C        ++EG  S + +     +
Sbjct: 330 GLCKKGQCNACPSDKGLLGWDETCKSPSLAS---CDPKTFHYFKIEGADSFMTKYNGGSS 389

BLAST of MS016774 vs. ExPASy Swiss-Prot
Match: Q9LZR8 (PAN domain-containing protein At5g03700 OS=Arabidopsis thaliana OX=3702 GN=At5g03700 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.2e-16
Identity = 87/368 (23.64%), Postives = 156/368 (42.39%), Query Frame = 0

Query: 111 DGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQ 170
           +G L +  P+  + W T T+G   ++L +    NL +V     ++W+SF+FP + ++  Q
Sbjct: 109 NGSLVIIDPSSRLEWSTHTNG---DRLILRNDSNLQVVKTSTFVEWESFDFPGNTLVESQ 168

Query: 171 SLNANTHLTSFPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP-------P 230
           +  +   L S            YS  +    + LY    S +S+  YW+ +         
Sbjct: 169 NFTSAMALVS--------PNGLYSMRLGSDFIGLYAKV-SEESQQFYWKHSALQAKAKVK 228

Query: 231 NNINLSFITLNTQG-LDIFSDQAKKIATIPSQS-EQPLRFLALGNTTGNLGLYSYSPQKG 290
           +        +N  G L ++   +  I      S ++P+  L +     +  L  Y     
Sbjct: 229 DGAGPILARINPNGYLGMYQTGSIPIDVEAFNSFQRPVNGLLILRLESDGNLRGYLWDGS 288

Query: 291 IFEASFRALKTTCDLPLACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCG-GIEA 350
            +  ++ A++ TCDLP  C PY +CT  + CSCI       +C     +   FC    E 
Sbjct: 289 HWALNYEAIRETCDLPNPCGPYSLCTPGSGCSCIDNRTVIGECTHAASSPADFCDKTTEF 348

Query: 351 EMIELEGISSILRE-APKRVNVSKEECGSWCLEDCKCAAALHYSGGGGGGGECYAYRVVM 410
           +++  +G+    +E    +   S  EC   C+++CKC  A++ +G G      Y  R ++
Sbjct: 349 KVVRRDGVEVPFKELMDHKTTSSLGECEEMCVDNCKCFGAVYNNGSGFCYLVNYPIRTML 408

Query: 411 GVKQIEREREMGLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFI 468
           GV    +     L Y  KV +  G+G+ KS +   +   +  V A +++     +G  F 
Sbjct: 409 GVADPSK-----LGYF-KVRE--GVGKKKSRVGLTVGMSLLAVIALVLMVAMVYVG--FR 454

BLAST of MS016774 vs. ExPASy Swiss-Prot
Match: Q39688 (Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.8e-15
Identity = 73/252 (28.97%), Postives = 108/252 (42.86%), Query Frame = 0

Query: 102 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNF 161
           E   L    DG+L L    G V W+T T+ +GV  L+IL  GN+ L D+     WQSF+ 
Sbjct: 102 ENATLTFGPDGNLVLARSNGQVAWQTSTANKGVVGLKILPNGNMVLYDSKGKFLWQSFDT 161

Query: 162 PTDVMLLGQSL--NANTHLTSFPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWE 221
           PTD +L+GQSL   A T L S   +   N    YS  +EP+ L LY    ++     Y+ 
Sbjct: 162 PTDTLLVGQSLKMGAVTKLVS-RASPGENVNGPYSLVMEPKGLHLYYKPTTSPKPIRYYS 221

Query: 222 FN---------PPNNINLSFITLNTQG------LDIFSDQAKKIATIPSQSE--QPLRFL 281
           F+            N+   F   N QG      L   +  +   A+I ++ +    L FL
Sbjct: 222 FSLFTKLNKNESLQNVTFEFENENDQGFAFLLSLKYGTSNSLGGASILNRIKYNTTLSFL 281

Query: 282 ALGNTTGNLGLYSYSPQ-----------------KGIFEASFRALK---TTCDLPLACKP 315
            L    GN+ +Y+Y+ +                   +F+ S  A +   + C LP  C  
Sbjct: 282 RL-EIDGNVKIYTYNDKVDYGAWEVTYTLFLKAPPPLFQVSLAATESESSECQLPKKCGN 341

BLAST of MS016774 vs. ExPASy TrEMBL
Match: A0A6J1DSQ6 (EP1-like glycoprotein 4 OS=Momordica charantia OX=3673 GN=LOC111024046 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 383/388 (98.71%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 60
           PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP
Sbjct: 52  PPMKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 111

Query: 61  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 120
           PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA
Sbjct: 112 PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 171

Query: 121 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 180
           GHVGWRTGTSGQGVEKLRILRTGNLALVDALE IKWQSFNFPTDVMLLGQSLNANTHLTS
Sbjct: 172 GHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTS 231

Query: 181 FPT--NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 240
           FPT  NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL
Sbjct: 232 FPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 291

Query: 241 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 300
           DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL
Sbjct: 292 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 351

Query: 301 ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 360
           ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILR APKR
Sbjct: 352 ACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILRGAPKR 411

Query: 361 VNVSKEECGSWCLEDCKCAAALHYSGGG 387
           VNVSKEECG WCLEDCKCAAALHYSGGG
Sbjct: 412 VNVSKEECGIWCLEDCKCAAALHYSGGG 439

BLAST of MS016774 vs. ExPASy TrEMBL
Match: A0A6J1I2F5 (PAN domain-containing protein At5g03700 OS=Cucurbita maxima OX=3661 GN=LOC111470282 PE=4 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.2e-204
Identity = 364/479 (75.99%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL    CRTDI  G+ VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVASAAQICF-VLLLLNLDRTICRTDIVPGHDVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+DQA KIA IPS + QPLRF+ALGN +GNLGLY YSPQKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDQAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSPQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEMGN----SKCGGEEINSGGFCGGIEAEMIELEGISSILREAP 362
           KPYGICTFSNSCSCI  ++ N    SKC  E   SG FCGGIE EM+ELEGISSILR+AP
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDSSKCSDE--ISGKFCGGIEGEMVELEGISSILRDAP 360

Query: 363 KRVNVSKEECGSWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMGL 422
            RVN+SK ECG+WCLEDCKCAAALHYSGGG    GGG ECY YR+VMGVK+I  E+ MG 
Sbjct: 361 NRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRLVMGVKEI--EKGMGF 420

Query: 423 SYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDT 472
           SYMVKVPKG+ L R KS LKKW+L VVGVVD  +I+AV GGLGYYFI RRR NL+  DT
Sbjct: 421 SYMVKVPKGTALERRKSGLKKWVLAVVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRDT 468

BLAST of MS016774 vs. ExPASy TrEMBL
Match: A0A6J1EZB2 (EP1-like glycoprotein 4 OS=Cucurbita moschata OX=3662 GN=LOC111440327 PE=4 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 2.0e-203
Identity = 363/482 (75.31%), Postives = 404/482 (83.82%), Query Frame = 0

Query: 3   MKV--AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 62
           MKV  AAQ CF +++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVGSAAQICF-VLLLLNLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 63  PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 122
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 123 GHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTS 182
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+G+KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 183 FPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 242
           FP     N+TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDI
Sbjct: 181 FP----PNSTSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDI 240

Query: 243 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 302
           F+D+A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLAC
Sbjct: 241 FNDEAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLAC 300

Query: 303 KPYGICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREA 362
           KPYGICTFSNSCSCI  ++      NSKC  E   SG FCGGIE EM+ELEGISSILR+A
Sbjct: 301 KPYGICTFSNSCSCITFQVENEGDDNSKCSDE--ISGKFCGGIEGEMVELEGISSILRDA 360

Query: 363 PKRVNVSKEECGSWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMG 422
           P RVN+SK ECG+WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+I  E+ MG
Sbjct: 361 PNRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEI--EKGMG 420

Query: 423 LSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDT 474
            SYMVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT
Sbjct: 421 FSYMVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILGDT 471

BLAST of MS016774 vs. ExPASy TrEMBL
Match: A0A5D3DNN1 (PAN domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00330 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.9e-191
Identity = 343/472 (72.67%), Postives = 380/472 (80.51%), Query Frame = 0

Query: 3   MKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPS 62
           MKVA  FC L +  FNL  +  RTD+ TGY+V LAVPAEY EGFIGRAFL+E++ + PP+
Sbjct: 1   MKVAPGFCLLFIFCFNLSPSISRTDLVTGYEVHLAVPAEYVEGFIGRAFLMESENLMPPN 60

Query: 63  FRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGH 122
           FRAALA+EA +G +SCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GH
Sbjct: 61  FRAALAIEATQGQYSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTADGDLRLKGPTGH 120

Query: 123 VGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTSFP 182
           VGWRTGTS QGVE+LRILR GNLALVDA+EGIKWQSFNFPTDVM+LGQSLN  THLTSFP
Sbjct: 121 VGWRTGTSRQGVERLRILRNGNLALVDAIEGIKWQSFNFPTDVMVLGQSLNVKTHLTSFP 180

Query: 183 TNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFS 242
                ++  FYSFEI+ QR+ALY+  NS K KYSYWEF PPNNINLS+ITLN +GLD F 
Sbjct: 181 ----PHSIFFYSFEIQTQRIALYL--NSPKCKYSYWEFKPPNNINLSYITLNPEGLDFFD 240

Query: 243 DQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKP 302
           D+A KIATIPS +  PLRFLALGN TGNLGLYSYSPQ GIFEASFRAL TTCDLPLACKP
Sbjct: 241 DRANKIATIPSGTPHPLRFLALGNKTGNLGLYSYSPQNGIFEASFRALTTTCDLPLACKP 300

Query: 303 YGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVNVS 362
           YGICTFSNSCSCI      SKC   E   G FC   + EM+EL G+SSILR+ PKRVNVS
Sbjct: 301 YGICTFSNSCSCI-----GSKC--REEMGGEFCEA-KGEMMELVGVSSILRDGPKRVNVS 360

Query: 363 KEECGSWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYMVKVPKGS 422
           KEECG WCL+DCKC AALHYS       ECY YRVV+GVKQI  E+ MGLSYMVKVPKG+
Sbjct: 361 KEECGEWCLDDCKCVAALHYS----VMEECYLYRVVIGVKQI--EKGMGLSYMVKVPKGT 420

Query: 423 GLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRR-NNLVDTDTHS 474
            LG HKS LK+W+L VVGVVD  +ILAV GGL YYF+ RRR  NL DTD HS
Sbjct: 421 ALGSHKSGLKRWVLAVVGVVDGVVILAVSGGLAYYFVKRRRKKNLTDTDVHS 452

BLAST of MS016774 vs. ExPASy TrEMBL
Match: A0A5A7TQ35 (PAN domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005760 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 5.1e-191
Identity = 344/472 (72.88%), Postives = 379/472 (80.30%), Query Frame = 0

Query: 3   MKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPS 62
           MKVA  FC L +  FNL  +  RTD+ TGY+V LAVPAEY EGFIGRAFL+E++ + PP+
Sbjct: 1   MKVAPGFCLLFIFCFNLSPSISRTDLVTGYEVHLAVPAEYVEGFIGRAFLMESENLMPPN 60

Query: 63  FRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGH 122
           FRAALA+EA +G +SCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GH
Sbjct: 61  FRAALAIEATQGQYSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTADGDLRLKGPTGH 120

Query: 123 VGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTSFP 182
           VGWRTGTS QGVE+LRILR GNLALVDA+EGIKWQSFNFPTDVM+LGQSLN  THLTSFP
Sbjct: 121 VGWRTGTSRQGVERLRILRNGNLALVDAIEGIKWQSFNFPTDVMVLGQSLNVKTHLTSFP 180

Query: 183 TNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFS 242
                ++  FYSFEI+ QR+ALY   NS K KYSYWEF PPNNINLS+ITLN +GLD F 
Sbjct: 181 ----PHSIFFYSFEIQTQRIALYF--NSPKCKYSYWEFKPPNNINLSYITLNPEGLDFFD 240

Query: 243 DQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKP 302
           D+A KIATIPS +  PLRFLALGN TGNLGLYSYSPQ GIFEASFRAL TTCDLPLACKP
Sbjct: 241 DRANKIATIPSGTPHPLRFLALGNKTGNLGLYSYSPQNGIFEASFRALTTTCDLPLACKP 300

Query: 303 YGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVNVS 362
           YGICTFSNSCSCI      SKC   E   G FC   + EM+EL G+SSILR+ PKRVNVS
Sbjct: 301 YGICTFSNSCSCI-----GSKC--REEMGGEFCEA-KGEMMELVGVSSILRDGPKRVNVS 360

Query: 363 KEECGSWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYMVKVPKGS 422
           KEECG WCL+DCKC AALHYS       ECY YRVV+GVKQI  E+ MGLSYMVKVPKG+
Sbjct: 361 KEECGEWCLDDCKCVAALHYS----VMEECYLYRVVIGVKQI--EKGMGLSYMVKVPKGT 420

Query: 423 GLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFI-NRRRNNLVDTDTHS 474
            LG HKS LK+W+L VVGVVD  +ILAV GGL YYFI  RRR NL DTD HS
Sbjct: 421 ALGSHKSGLKRWVLAVVGVVDGVVILAVSGGLAYYFIKRRRRKNLTDTDVHS 452

BLAST of MS016774 vs. TAIR 10
Match: AT3G51710.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 460.7 bits (1184), Expect = 1.4e-129
Identity = 244/470 (51.91%), Postives = 319/470 (67.87%), Query Frame = 0

Query: 9   FCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQIT--PPSFRAA 68
           F FLI  LF+    HC++DI  G  +TL  P EY  GF+G+A++IET+  +   P F+AA
Sbjct: 8   FTFLI-CLFSKLQGHCKSDISLGNSLTLTSPLEYTPGFMGKAYIIETESSSTREPGFKAA 67

Query: 69  LAVEAI---EGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHV 128
           L +E+    +G + CSLQ+FLG V+VWSSGH+S+ Y + KC++ELT DGDLRLK    HV
Sbjct: 68  LTMESSDKDDGRYLCSLQIFLGDVRVWSSGHYSKMYVSSKCIIELTKDGDLRLKSSYKHV 127

Query: 129 GWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQSLNANTHLTSFPT 188
           GWR+GTSGQGVE+L I  TGNL LVDA   IKWQSFNFPTDVML GQ L+  T LTSFP 
Sbjct: 128 GWRSGTSGQGVERLEIQSTGNLVLVDAKNLIKWQSFNFPTDVMLSGQRLDVATQLTSFPN 187

Query: 189 NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP-PNNINLSFITLNTQGLDIFS 248
               ++T FYSFE+   ++AL++N N  K KYSYWE+ P   N  ++F+ L  +GLD+F 
Sbjct: 188 ----DSTLFYSFEVLRDKIALFLNLN--KLKYSYWEYKPREKNTTVNFVRLGLKGLDLFD 247

Query: 249 DQAKKIATIPSQSEQPL-RFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACK 308
           D ++ I  I    EQPL RFLALGN TGNLGLYSY P+KG FEA+F+A+  TCDLP+ACK
Sbjct: 248 DNSRIIGRI----EQPLIRFLALGNRTGNLGLYSYKPEKGKFEATFQAVSDTCDLPVACK 307

Query: 309 PYGICTFSNSCSCIRVEMGNSKC----GGEEINSGGFCGGIEAEMIELEGISSILREAPK 368
           PYGICTFS SCSCI+V + N  C    G E ++    C   + EM+EL G++++LR   +
Sbjct: 308 PYGICTFSKSCSCIKV-VSNGYCSSINGEEAVSVKRLC---DHEMVELNGVTTVLRNGTQ 367

Query: 369 RVNVSKEECGSWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYMVK 428
             N+SKE C   C +DC+C AA +          C  Y +VMGVKQI  ER  GLSYMVK
Sbjct: 368 VRNISKERCEELCKKDCECGAASY----SVSEESCVMYGIVMGVKQI--ERVSGLSYMVK 427

Query: 429 VPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLV 468
           +PKG  L   KS+++KW++ +VG +D F+IL +  G  +YFI +RR +L+
Sbjct: 428 IPKGVRLSDEKSNVRKWVVGLVGGIDGFVILLLISGFAFYFIRKRRKSLL 456

BLAST of MS016774 vs. TAIR 10
Match: AT1G78860.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-21
Identity = 87/318 (27.36%), Postives = 138/318 (43.40%), Query Frame = 0

Query: 89  VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 148
           VW +   S     E   L    DG+L L    G V W+T T+ +GV  ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVGIKILENGNMVIY 149

Query: 149 DALEGIKWQSFNFPTDVMLLGQS--LNANTHLTSFPTNANANATSFYSFEIEPQRLALYI 208
           D+     WQSF+ PTD +L+GQS  LN    L S   + + NA   YS  +E ++L LY 
Sbjct: 150 DSNGKFVWQSFDSPTDTLLVGQSLKLNGQNKLVS-RLSPSVNANGPYSLVMEAKKLVLYY 209

Query: 209 NSNSAKSKYSYWEFNPPNNI-NLSFIT------------LNTQGLDIFSDQAKKIATIPS 268
            +N       Y+E+     I  L  +T            L+ +G+D  S     ++T  S
Sbjct: 210 TTNKTPKPIGYYEYEFFTKIAQLQSMTFQAVEDADTTWGLHMEGVD--SGSQFNVSTFLS 269

Query: 269 QSEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRAL-------KTTCDLPLACKP 328
           + +    L FL L  + GN+ ++SYS       ++ ++ A           C +P  C  
Sbjct: 270 RPKHNATLSFLRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDGNDECRIPEHCLG 329

Query: 329 YGICTFSNSCSCIRVEMG----NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 377
           +G+C     C+    ++G    +  C   +I S   C        ++EG  S + +    
Sbjct: 330 FGLCK-KGQCNACPSDIGLLGWDETC---KIPSLASCDPKTFHYFKIEGADSFMTKYNGG 389

BLAST of MS016774 vs. TAIR 10
Match: AT1G78830.1 (Curculin-like (mannose-binding) lectin family protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.9e-20
Identity = 86/319 (26.96%), Postives = 128/319 (40.13%), Query Frame = 0

Query: 102 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNF 161
           E   L L  +G+L L    G V W+T T+ +GV   +IL  GN+ L D      WQSF+ 
Sbjct: 105 ENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDH 164

Query: 162 PTDVMLLGQSLNAN--THLTSFPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWE 221
           PTD +L GQSL  N    L S  +++N  +   YS  ++ + L +Y+N       Y  W 
Sbjct: 165 PTDTLLTGQSLKVNGVNKLVSRTSDSN-GSDGPYSMVLDKKGLTMYVNKTGTPLVYGGWP 224

Query: 222 FNPPNNINLSFITLNTQGL------DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGL 281
            +         +T     L      ++  + A + AT P  + + L+   +G+  G L L
Sbjct: 225 DHDFRGTVTFAVTREFDNLTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTLNL 284

Query: 282 ----------------------YSYSPQKGI--FEASFRALKT----TCDLPLACKPYGI 341
                                 YSY P      +E SF    T     C LP  C  YG 
Sbjct: 285 NKINYNGTISYLRLGSDGSLKAYSYFPAATYLKWEESFSFFSTYFVRQCGLPSFCGDYGY 344

Query: 342 CT--FSNSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN-- 377
           C     N+C   +  +G + KC   +     FC G++ + +    I  +       VN  
Sbjct: 345 CDRGMCNACPTPKGLLGWSDKCAPPKTTQ--FCSGVKGKTVNYYKIVGVEHFTGPYVNDG 404

BLAST of MS016774 vs. TAIR 10
Match: AT1G78850.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 97.8 bits (242), Expect = 2.4e-20
Identity = 81/316 (25.63%), Postives = 137/316 (43.35%), Query Frame = 0

Query: 89  VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 148
           VW +   S     E   L    DG+L L    G + W+T T+ +G   ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRLVWQTNTANKGAVGIKILENGNMVIY 149

Query: 149 DALEGIKWQSFNFPTDVMLLGQS--LNANTHLTSFPTNANANATSFYSFEIEPQRLALYI 208
           D+     WQSF+ PTD +L+GQS  LN  T L S   + + N    YS  +E ++L LY 
Sbjct: 150 DSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKLVS-RLSPSVNTNGPYSLVMEAKKLVLYY 209

Query: 209 NSNSAKSKYSYWEFNPPNNINLSFITLNTQGLD------------IFSDQAKKIATIPSQ 268
            +N      +Y+E+     I   F ++  Q ++            + S     ++T  S+
Sbjct: 210 TTNKTPKPIAYFEYEFFTKIT-QFQSMTFQAVEDSDTTWGLVMEGVDSGSKFNVSTFLSR 269

Query: 269 SEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRALKTT-------CDLPLACKPY 328
            +    L F+ L  + GN+ ++SYS       ++ ++ A           C +P  C  +
Sbjct: 270 PKHNATLSFIRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNADTDGNDECRIPEHCLGF 329

Query: 329 GICTFS--NSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 377
           G+C     N+C   +  +G +  C    + S   C        ++EG  S + +     +
Sbjct: 330 GLCKKGQCNACPSDKGLLGWDETCKSPSLAS---CDPKTFHYFKIEGADSFMTKYNGGSS 389

BLAST of MS016774 vs. TAIR 10
Match: AT5G03700.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 89.4 bits (220), Expect = 8.7e-18
Identity = 87/368 (23.64%), Postives = 156/368 (42.39%), Query Frame = 0

Query: 111 DGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALEGIKWQSFNFPTDVMLLGQ 170
           +G L +  P+  + W T T+G   ++L +    NL +V     ++W+SF+FP + ++  Q
Sbjct: 109 NGSLVIIDPSSRLEWSTHTNG---DRLILRNDSNLQVVKTSTFVEWESFDFPGNTLVESQ 168

Query: 171 SLNANTHLTSFPTNANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP-------P 230
           +  +   L S            YS  +    + LY    S +S+  YW+ +         
Sbjct: 169 NFTSAMALVS--------PNGLYSMRLGSDFIGLYAKV-SEESQQFYWKHSALQAKAKVK 228

Query: 231 NNINLSFITLNTQG-LDIFSDQAKKIATIPSQS-EQPLRFLALGNTTGNLGLYSYSPQKG 290
           +        +N  G L ++   +  I      S ++P+  L +     +  L  Y     
Sbjct: 229 DGAGPILARINPNGYLGMYQTGSIPIDVEAFNSFQRPVNGLLILRLESDGNLRGYLWDGS 288

Query: 291 IFEASFRALKTTCDLPLACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCG-GIEA 350
            +  ++ A++ TCDLP  C PY +CT  + CSCI       +C     +   FC    E 
Sbjct: 289 HWALNYEAIRETCDLPNPCGPYSLCTPGSGCSCIDNRTVIGECTHAASSPADFCDKTTEF 348

Query: 351 EMIELEGISSILRE-APKRVNVSKEECGSWCLEDCKCAAALHYSGGGGGGGECYAYRVVM 410
           +++  +G+    +E    +   S  EC   C+++CKC  A++ +G G      Y  R ++
Sbjct: 349 KVVRRDGVEVPFKELMDHKTTSSLGECEEMCVDNCKCFGAVYNNGSGFCYLVNYPIRTML 408

Query: 411 GVKQIEREREMGLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFI 468
           GV    +     L Y  KV +  G+G+ KS +   +   +  V A +++     +G  F 
Sbjct: 409 GVADPSK-----LGYF-KVRE--GVGKKKSRVGLTVGMSLLAVIALVLMVAMVYVG--FR 454