Homology
BLAST of MS016603 vs. NCBI nr
Match:
XP_022150068.1 (alpha-dioxygenase 1-like [Momordica charantia])
HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 635/638 (99.53%), Postives = 635/638 (99.53%), Query Frame = 0
Query: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR
Sbjct: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
Query: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT
Sbjct: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
Query: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE
Sbjct: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
Query: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD
Sbjct: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
Query: 241 GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED 300
GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED
Sbjct: 241 GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED 300
Query: 301 LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG 360
LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG
Sbjct: 301 LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG 360
Query: 361 GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN 420
GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN
Sbjct: 361 GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN 420
Query: 421 MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA 480
MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA
Sbjct: 421 MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA 480
Query: 481 LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM 540
LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM
Sbjct: 481 LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM 540
Query: 541 AEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIE 600
AEKKIKGFAISETAFVIFLLMASRRLEAD FFTSHFNEETYTKKGLEWVNTTESLKDVIE
Sbjct: 541 AEKKIKGFAISETAFVIFLLMASRRLEADTFFTSHFNEETYTKKGLEWVNTTESLKDVIE 600
Query: 601 RHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIPS 639
RHHPDI NKWMNSSSAFSVWDSPPNK NPIPIYLRIPS
Sbjct: 601 RHHPDIANKWMNSSSAFSVWDSPPNKRNPIPIYLRIPS 638
BLAST of MS016603 vs. NCBI nr
Match:
XP_038903382.1 (alpha-dioxygenase 1-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 571/636 (89.78%), Postives = 609/636 (95.75%), Query Frame = 0
Query: 2 LSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRR 61
+ +LR +LQKFIH+DFHE V+RMTVIDT LFLI+H +DKLGVWHKLPVILGL+YLA+RR
Sbjct: 9 IKAVLRGELQKFIHKDFHEVVQRMTVIDTFLFLIIHFIDKLGVWHKLPVILGLLYLAVRR 68
Query: 62 HLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQTK 121
HLHQEYNLFNVGRTPVGVRFNP+DFPYRTADG YNDPFNE AGAQG+FFGRNI PVDQ+K
Sbjct: 69 HLHQEYNLFNVGRTPVGVRFNPMDFPYRTADGKYNDPFNEGAGAQGTFFGRNIHPVDQSK 128
Query: 122 KLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEV 181
LLKPDPMVVATKLL RRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV+LVAP EV
Sbjct: 129 MLLKPDPMVVATKLLTRRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVDLVAPREV 188
Query: 182 ATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDG 241
A ++CPLKSFKFFKTKQV TGFFE KTGS+NVRTPWWDGSVLYGSN+EKLGKVRTY+DG
Sbjct: 189 A--SECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKVRTYKDG 248
Query: 242 KLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDL 301
KLKIADDGLLLHD+DGVAISGDVRNSWAGVSTLQALFIKEHNA+CD LKK D +LEDEDL
Sbjct: 249 KLKIADDGLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAICDALKKADEDLEDEDL 308
Query: 302 YRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGG 361
YRYARLVTSAVIAK+HTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGG+I GG
Sbjct: 309 YRYARLVTSAVIAKVHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGAILGG 368
Query: 362 LVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNM 421
LVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDFYLRDV++DP+ NKSPPLI KVPMGNM
Sbjct: 369 LVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFYLRDVSVDPNHNKSPPLIEKVPMGNM 428
Query: 422 IGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAAL 481
IGH GEEASK+MGFTALLVSMGHQ+SGALELWNYP+WLRDL HD+DGKDRPDHVDLAAL
Sbjct: 429 IGHKGEEASKEMGFTALLVSMGHQSSGALELWNYPVWLRDLIAHDMDGKDRPDHVDLAAL 488
Query: 482 EVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMA 541
EVYRDRER+VARYNDFRRGL LIPISKWEDLTD+ EAIEVLRDVYG+DVEELDILVGLMA
Sbjct: 489 EVYRDRERRVARYNDFRRGLFLIPISKWEDLTDNKEAIEVLRDVYGDDVEELDILVGLMA 548
Query: 542 EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER 601
EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER
Sbjct: 549 EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER 608
Query: 602 HHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
HHP+I+NKWMNSSSAFSVWDSPPNKPNPIPIYLR+P
Sbjct: 609 HHPEISNKWMNSSSAFSVWDSPPNKPNPIPIYLRLP 642
BLAST of MS016603 vs. NCBI nr
Match:
XP_038903383.1 (alpha-dioxygenase 1-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1186.8 bits (3069), Expect = 0.0e+00
Identity = 570/636 (89.62%), Postives = 608/636 (95.60%), Query Frame = 0
Query: 2 LSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRR 61
+ +LR +LQKFIH+DFHE V+RMTVIDT LFLI+H +DKLGVWHKLPVILGL+YLA+RR
Sbjct: 9 IKAVLRGELQKFIHKDFHEVVQRMTVIDTFLFLIIHFIDKLGVWHKLPVILGLLYLAVRR 68
Query: 62 HLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQTK 121
HLHQEYNLFNVGRTPVGVRFNP+DFPYRTADG YNDPFNE AGAQG+FFGRNI PVDQ+K
Sbjct: 69 HLHQEYNLFNVGRTPVGVRFNPMDFPYRTADGKYNDPFNEGAGAQGTFFGRNIHPVDQSK 128
Query: 122 KLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEV 181
LLKPDPMVVATKLL RRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV+LVAP EV
Sbjct: 129 MLLKPDPMVVATKLLTRRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVDLVAPREV 188
Query: 182 ATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDG 241
A ++CPLKSFKFFKTKQV TGFFE KTGS+NVRTPWWDGSVLYGSN+EKLGKVRTY+DG
Sbjct: 189 A--SECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKVRTYKDG 248
Query: 242 KLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDL 301
KLKIADDGLLLHD+DGVAISGDVRNSWAGVSTLQALFIKEHNA+CD L K D +LEDEDL
Sbjct: 249 KLKIADDGLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAICDAL-KADEDLEDEDL 308
Query: 302 YRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGG 361
YRYARLVTSAVIAK+HTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGG+I GG
Sbjct: 309 YRYARLVTSAVIAKVHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGAILGG 368
Query: 362 LVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNM 421
LVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDFYLRDV++DP+ NKSPPLI KVPMGNM
Sbjct: 369 LVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFYLRDVSVDPNHNKSPPLIEKVPMGNM 428
Query: 422 IGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAAL 481
IGH GEEASK+MGFTALLVSMGHQ+SGALELWNYP+WLRDL HD+DGKDRPDHVDLAAL
Sbjct: 429 IGHKGEEASKEMGFTALLVSMGHQSSGALELWNYPVWLRDLIAHDMDGKDRPDHVDLAAL 488
Query: 482 EVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMA 541
EVYRDRER+VARYNDFRRGL LIPISKWEDLTD+ EAIEVLRDVYG+DVEELDILVGLMA
Sbjct: 489 EVYRDRERRVARYNDFRRGLFLIPISKWEDLTDNKEAIEVLRDVYGDDVEELDILVGLMA 548
Query: 542 EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER 601
EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER
Sbjct: 549 EKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIER 608
Query: 602 HHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
HHP+I+NKWMNSSSAFSVWDSPPNKPNPIPIYLR+P
Sbjct: 609 HHPEISNKWMNSSSAFSVWDSPPNKPNPIPIYLRLP 641
BLAST of MS016603 vs. NCBI nr
Match:
XP_008443484.1 (PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 [Cucumis melo var. makuwa])
HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 574/644 (89.13%), Postives = 608/644 (94.41%), Query Frame = 0
Query: 1 MLSQL-------LRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILG 60
MLSQL LR +LQKF+H+DFHE V+RMTVIDT LFLI+H VDKLG+WHKLPVILG
Sbjct: 1 MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60
Query: 61 LVYLAIRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRN 120
L+YLA+RRHLHQEYNLFNVGRTPVGVRFNPVD+PYRTADG YNDPFNE AGAQGSFFGRN
Sbjct: 61 LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120
Query: 121 ILPVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
I PVDQ+K LLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
Query: 181 ELVAPHEVATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLG 240
ELVAP EVA ++CPLKSFKFFKTKQV TGFFE KTGS+NVRTPWWDGSVLYGSN+EKLG
Sbjct: 181 ELVAPSEVA--SECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLG 240
Query: 241 KVRTYRDGKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKED 300
KVRTY+DGKLKIADD LLLHD+DGVAISGDVRNSWAGVSTLQALFIKEHNAVCD LKKED
Sbjct: 241 KVRTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKED 300
Query: 301 PELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGH 360
LEDEDLYRYARLVTSAVIAK+HTIDWTVELLK DTLLAGMRGNWYGLLGKKFKDTFGH
Sbjct: 301 ENLEDEDLYRYARLVTSAVIAKVHTIDWTVELLKMDTLLAGMRGNWYGLLGKKFKDTFGH 360
Query: 361 VGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLI 420
VGG+I GGLVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDFYLRDV++DP +KSPPL+
Sbjct: 361 VGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFYLRDVSVDPDHDKSPPLV 420
Query: 421 NKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRP 480
KVPM +MIGH GE SKKMGFTALLVSMGHQASGALELWNYPLWLRDL HD+DGKDRP
Sbjct: 421 EKVPMSDMIGHKGEVTSKKMGFTALLVSMGHQASGALELWNYPLWLRDLIAHDMDGKDRP 480
Query: 481 DHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEEL 540
DHVDLAALEVYRDRER+VARYNDFRRGL LIPISKWEDLTDD+EAIEVLR+VYG+DVEEL
Sbjct: 481 DHVDLAALEVYRDRERRVARYNDFRRGLFLIPISKWEDLTDDEEAIEVLREVYGDDVEEL 540
Query: 541 DILVGLMAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
DILVGLMAEKKIKGFAISETAFVIFL+MASRRLEADRFFTSHFNEETYTKKGLEWVNTTE
Sbjct: 541 DILVGLMAEKKIKGFAISETAFVIFLIMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
Query: 601 SLKDVIERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
SLKDVIERH+P+I+NKWMNSSSAFSVWDSPPNKPNPIPIYLRIP
Sbjct: 601 SLKDVIERHYPEISNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 642
BLAST of MS016603 vs. NCBI nr
Match:
XP_004147441.2 (alpha-dioxygenase 1 [Cucumis sativus] >KGN59637.1 hypothetical protein Csa_002419 [Cucumis sativus])
HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 569/644 (88.35%), Postives = 607/644 (94.25%), Query Frame = 0
Query: 1 MLSQL-------LRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILG 60
MLSQL LR +LQKF+H+DFHE V+RMTVIDT LFLIVH VDKLG+WHKLPVILG
Sbjct: 1 MLSQLLKPIKIVLRGELQKFVHKDFHEVVQRMTVIDTFLFLIVHFVDKLGIWHKLPVILG 60
Query: 61 LVYLAIRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRN 120
L+YLA+RRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADG YNDPFNE AGAQGSFFGRN
Sbjct: 61 LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGKYNDPFNEGAGAQGSFFGRN 120
Query: 121 ILPVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
I PVDQ+K LLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
Query: 181 ELVAPHEVATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLG 240
ELVAP EVA ++CPLKSFKFFKTKQV TGFF+ KTGS+NVRTPWWDGSVLYGSN+EKLG
Sbjct: 181 ELVAPREVA--SECPLKSFKFFKTKQVSTGFFDNKTGSINVRTPWWDGSVLYGSNAEKLG 240
Query: 241 KVRTYRDGKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKED 300
KVRTY+DGKLKIADD LLLHD+DGVAISGDVRNSWAGVSTLQALFI+EHNAVCD LKKED
Sbjct: 241 KVRTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIREHNAVCDALKKED 300
Query: 301 PELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGH 360
+LEDEDLYRYARLVTSAVIAK+HTIDWTVELLK DTLLAGMRGNWYGLLGKKFKDTFGH
Sbjct: 301 EDLEDEDLYRYARLVTSAVIAKVHTIDWTVELLKMDTLLAGMRGNWYGLLGKKFKDTFGH 360
Query: 361 VGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLI 420
VGG+I GGLVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDF+LRD+++DP NKSPPL+
Sbjct: 361 VGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFHLRDISVDPDHNKSPPLV 420
Query: 421 NKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRP 480
KVPM NMIGH GEE SKKMGFTALLVSMGHQ+SGALELWNYP WLRDL PHD+DGKDR
Sbjct: 421 EKVPMANMIGHKGEETSKKMGFTALLVSMGHQSSGALELWNYPQWLRDLIPHDMDGKDRA 480
Query: 481 DHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEEL 540
DH+DLAALEVYRDRER+VARYNDFRRGL LIPISKWEDLTDD+ AIEVLR+VYG+DVEEL
Sbjct: 481 DHIDLAALEVYRDRERRVARYNDFRRGLFLIPISKWEDLTDDEGAIEVLREVYGDDVEEL 540
Query: 541 DILVGLMAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
DILVGLMAEKKIKGFAISETAFVIFL+MASRRLEADRFFTSHFNEETYTKKGLEWVNTTE
Sbjct: 541 DILVGLMAEKKIKGFAISETAFVIFLIMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
Query: 601 SLKDVIERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
SLKDVIERH+P+I+NKWMNSSSAFSVWDSPPNKPNPIPIY R+P
Sbjct: 601 SLKDVIERHYPEISNKWMNSSSAFSVWDSPPNKPNPIPIYFRLP 642
BLAST of MS016603 vs. ExPASy Swiss-Prot
Match:
Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)
HSP 1 Score: 1005.7 bits (2599), Expect = 2.3e-292
Identity = 478/639 (74.80%), Postives = 549/639 (85.92%), Query Frame = 0
Query: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
+++ L+ S L KFIH+DFHE RM+++D L LIVH VDK+ WHKLPV LGL YL +R
Sbjct: 3 VITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVR 62
Query: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
RHLHQ+YNL NVG+TP G+RF+P ++PYRTADG +NDPFNE G+Q SFFGRN PVDQ
Sbjct: 63 RHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQK 122
Query: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
KL +PDPMVVATKLL R+K IDTGKQFNMIAASWIQFMIHDWIDHLEDT Q+ELVAP E
Sbjct: 123 SKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKE 182
Query: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
VA ++CPL SF+F KTK+VPTGFFEIKTGS N+RTPWWD SV+YGSNS+ L +VRTY+D
Sbjct: 183 VA--SKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKD 242
Query: 241 GKLKIADD-GLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDE 300
GKLKI+++ GLLLHD DG+AISGD+RNSWAGVS LQALFIKEHNAVCD LK ED +LEDE
Sbjct: 243 GKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDE 302
Query: 301 DLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIF 360
DLYRYARLVTSAV+AKIHTIDWTV+LLKTDTLLAGMR NWYGLLGKKFKD+FGH G SI
Sbjct: 303 DLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSIL 362
Query: 361 GGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMG 420
GG+VG+KKP NHG+PYSLTE+F SVYRMHSLLPD ++ D+ P NKS PLI ++ M
Sbjct: 363 GGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQEISMR 422
Query: 421 NMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLA 480
++IG GEE +GFT L+VSMGHQASGALEL NYP+WLRD+ PHD +G+ RPDHVDLA
Sbjct: 423 DLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLA 482
Query: 481 ALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGL 540
ALE+YRDRER V RYN+FRR + +IPI+KWEDLT+D+EAIEVL DVY DVEELD+LVGL
Sbjct: 483 ALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGL 542
Query: 541 MAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVI 600
MAEKKIKGFAISETAF IFL+MA+RRLEADRFFTS FNE YTKKGLEWVNTTESLKDVI
Sbjct: 543 MAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWVNTTESLKDVI 602
Query: 601 ERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIPS 639
+RH+PD+T+KWMNS SAFSVWDSPP NPIP+YLRIPS
Sbjct: 603 DRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLRIPS 639
BLAST of MS016603 vs. ExPASy Swiss-Prot
Match:
Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)
HSP 1 Score: 819.7 bits (2116), Expect = 2.3e-236
Identity = 395/624 (63.30%), Postives = 485/624 (77.72%), Query Frame = 0
Query: 13 FIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRRHLHQEYNLFNV 72
F+H H V +M+ D LF IVHLVDKLG+WH+ PV+LG+ YL +RRHLHQ YNL +V
Sbjct: 10 FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69
Query: 73 GRTPV-GVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQTKKLLKPDPMVV 132
G P+ G ++ +F YRTADG N P + G+QGSF GRN+ P +L P P VV
Sbjct: 70 G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVV 129
Query: 133 ATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEVATSNQCPLKS 192
ATKLLAR++ ID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA+ CPLKS
Sbjct: 130 ATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASG--CPLKS 189
Query: 193 FKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLKIADDGLL 252
FKF +TK+VPT K+G+VN RTPWWDGSV+YG++ + +VR ++DGKLKI+ DGLL
Sbjct: 190 FKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKISGDGLL 249
Query: 253 LHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDLYRYARLVTSA 312
D GV ISGD+RNSW+G S LQALF+KEHN+VCD LK+ P+ +DE LYR ARLVT+A
Sbjct: 250 ERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTARLVTAA 309
Query: 313 VIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGLVGLKKPNNH 372
VIAK+HTIDWT+ELLKTDTL AGMR NWYG GKK KD G G +F GLVGLKKPN+H
Sbjct: 310 VIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGPLFSGLVGLKKPNDH 369
Query: 373 GIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNMIGHNGEEASK 432
G+PYSLTEEF SVYRMH LLP+ LRD+ + ++P + ++PM +IG E +
Sbjct: 370 GVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIEREIPMTELIGKKAGEKAS 429
Query: 433 KMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKV 492
K+GF LLVSMGHQ+ GAL LWNYP W+R+L D+DG+DRP +D+AALE+YRDRER V
Sbjct: 430 KLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALEIYRDRERGV 489
Query: 493 ARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGFAIS 552
RYN+FR+ LL+ PISKWE+LTDD+EAI+VLR+VY +D+E+LD+ VGL AEKKIKGFAIS
Sbjct: 490 PRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAEKKIKGFAIS 549
Query: 553 ETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIERHHPDITNKWM 612
ETAF IFLL+ASRRLEADRFFT++FNE+TYTK+GLEWVNTTE+LKDVI+RH P +T++WM
Sbjct: 550 ETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTTETLKDVIDRHFPRLTDQWM 609
Query: 613 NSSSAFSVWDSPPNKPNPIPIYLR 636
SSAFSVW S PN N +P+YLR
Sbjct: 610 RCSSAFSVWGSDPNPKNWVPLYLR 628
BLAST of MS016603 vs. ExPASy Swiss-Prot
Match:
P27607 (Prostaglandin G/H synthase 2 OS=Gallus gallus OX=9031 GN=PTGS2 PE=2 SV=1)
HSP 1 Score: 82.4 bits (202), Expect = 2.0e-14
Identity = 118/526 (22.43%), Postives = 200/526 (38.02%), Query Frame = 0
Query: 108 SFFGRNILPVD---------QTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQF 167
S++ R++ PV + KK L PD ++ K L RRK I + N++ + Q
Sbjct: 132 SYYTRSLPPVGHDCPTPMGVKGKKEL-PDSKLIVEKFLLRRKFIPDPQGTNVMFTFFAQH 191
Query: 168 MIHDWIDHLEDTKQVELVAPHEVATSNQCPLKSFKFFKT--KQVPTGFFEIKTGSVNVRT 227
H +FFKT K+ P GF + V
Sbjct: 192 FTH-------------------------------QFFKTDHKKGP-GFTKAYGHGV---- 251
Query: 228 PWWDGSVLYGSNSEKLGKVRTYRDGKLKI-ADDGLL-----------------LHDHDGV 287
D + +YG E+ K+R +DGKLK DG + + +H
Sbjct: 252 ---DLNHIYGETLERQLKLRLRKDGKLKYQMIDGEMYPPTVKDTQAEMIYPPHVPEHLQF 311
Query: 288 AISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDLYRYARLVTSAVIAKIHT 347
++ +V G+ ++++EHN VCD LK+E PE +DE L++ RL+ KI
Sbjct: 312 SVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWDDEQLFQTTRLILIGETIKIVI 371
Query: 348 IDWTVEL------LKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGLVGLKKPNNHG 407
D+ L LK D L L ++F+
Sbjct: 372 EDYVQHLSGYHFKLKFDPEL---------LFNQRFQ------------------------ 431
Query: 408 IPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNMIGHNGEEASKK 467
+ EF ++Y H LLPD F + + + + N + + + + H + SK+
Sbjct: 432 YQNRIAAEFNTLYHWHPLLPDTFQIHN---QEYTFQQFLYNNSIMLEHGLSHMVKSFSKQ 491
Query: 468 MGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKVA 527
S G A G N P ++ + +D + R+ +
Sbjct: 492 --------SAGRVAGGK----NVPAAVQKVAKASID----------------QSRQMRYQ 551
Query: 528 RYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGFAISE 587
N++R+ +L P +E+LT + E L ++YG D++ +++ GL+ EK G E
Sbjct: 552 SLNEYRKRFMLKPFKSFEELTGEKEMAAELEELYG-DIDAMELYPGLLVEKPRPGAIFGE 552
Query: 588 TAFVIFLLMASRRLEADRFFT-SHFNEETY-TKKGLEWVNTTESLK 597
T I + + L + + ++ T+ K G E +NT K
Sbjct: 612 TMVEIGAPFSLKGLMGNTICSPEYWKPSTFGGKVGFEIINTASLQK 552
BLAST of MS016603 vs. ExPASy Swiss-Prot
Match:
Q8HZR1 (Prostaglandin G/H synthase 1 OS=Canis lupus familiaris OX=9615 GN=PTGS1 PE=1 SV=2)
HSP 1 Score: 81.3 bits (199), Expect = 4.5e-14
Identity = 102/454 (22.47%), Postives = 175/454 (38.55%), Query Frame = 0
Query: 116 PVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVEL 175
P+ K PD ++ + L RRK I + N++ A + Q H
Sbjct: 165 PMGTKGKKQLPDAQLLGRRFLLRRKFIPDPQGTNLMFAFFAQHFTH-------------- 224
Query: 176 VAPHEVATSNQCPLKSFKFFKTK-QVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGK 235
+FFKT ++ GF + V D +YG N ++ +
Sbjct: 225 -----------------QFFKTSGKMGPGFTKALGHGV-------DLGHIYGDNLDRQYQ 284
Query: 236 VRTYRDGKLK------------IADDGLLLHDHDGV------AISGDVRNSWAGVSTLQA 295
+R ++DGKLK + + +L+H G+ A+ +V G+
Sbjct: 285 LRLFKDGKLKYQVLDGEMYPPSVEEAPVLMHYPRGILPQSQMAVGQEVFGLLPGLMLYAT 344
Query: 296 LFIKEHNAVCDTLKKEDPELEDEDLYRYARLVTSAVIAKIHTIDWTVEL------LKTDT 355
L+++EHN VCD LK E P DE L++ ARL+ KI ++ +L LK D
Sbjct: 345 LWLREHNRVCDLLKAEHPTWGDEQLFQTARLILIGETIKIVIEEYVQQLSGYFLQLKFDP 404
Query: 356 LLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSL 415
L L +F+ + EF +Y H L
Sbjct: 405 EL---------LFSAQFQ------------------------YRNRIAMEFNQLYHWHPL 464
Query: 416 LPDDFYLRDVTIDPHRNKSPPLINKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGAL 475
+PD F++ + L N +M+ H G E AL+ + Q++G +
Sbjct: 465 MPDSFWVG----SQEYSYEQFLFNT----SMLTHYGIE--------ALVDAFSRQSAGRI 514
Query: 476 ELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRD-RERKVARYNDFRRGLLLIPISKW 535
G++ HV A+E ++ RE ++ +N++R+ + P +
Sbjct: 525 G----------------GGRNIDHHVLHVAVETIKESRELRLQPFNEYRKRFGMRPYMSF 514
Query: 536 EDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEK 544
++LT + E L ++YG D++ L+ GL+ EK
Sbjct: 585 QELTGEKEMAAELEELYG-DIDALEFYPGLLLEK 514
BLAST of MS016603 vs. ExPASy Swiss-Prot
Match:
P70682 (Prostaglandin G/H synthase 2 OS=Cavia porcellus OX=10141 GN=PTGS2 PE=2 SV=1)
HSP 1 Score: 80.5 bits (197), Expect = 7.6e-14
Identity = 103/449 (22.94%), Postives = 165/449 (36.75%), Query Frame = 0
Query: 126 PDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEVATSN 185
PD V K+L RRK I + NM+ A + Q H
Sbjct: 158 PDSNEVLEKVLLRRKFIPDPQGTNMMFAFFAQHFTH------------------------ 217
Query: 186 QCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLK- 245
+FFK+ Q F TG + D S +YG ++ K+R ++DGK+K
Sbjct: 218 -------QFFKSDQKRGPAF--TTGLAH----GVDLSHIYGETLDRQHKLRLFKDGKMKY 277
Query: 246 -IADDGL----------------LLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCD 305
I D + + +H A+ +V G+ ++++EHN VCD
Sbjct: 278 QIIDGEMYPPTVKETQVEMMYPPYIPEHARFAVGQEVFGLVPGLMMYATIWLREHNRVCD 337
Query: 306 TLKKEDPELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKF 365
LK+E PE +DE L++ +RL+ KI
Sbjct: 338 VLKQEHPEWDDERLFQTSRLILIGETIKI-----------------------------VI 397
Query: 366 KDTFGHVGGSIFGGLVGLKKPNNHGIPYS--LTEEFASVYRMHSLLPDDFYLRDVTIDPH 425
+D H+ G F + N Y + EF ++Y H LLPD F + D
Sbjct: 398 EDYVQHLSGYHFKLKFDPELLFNQQFQYQNRIASEFNTLYHWHPLLPDTFQIDDQVY--- 457
Query: 426 RNKSPPLINKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPH 485
N L N ++ H G T + S Q +G +
Sbjct: 458 -NFQQFLYNN---SILVEH---------GLTQFVESFTKQIAGRV--------------- 507
Query: 486 DVDGKDRPDHVD-LAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRD 545
G++ P V +A + R+ K N++R+ L+ P + +E+LT + E L
Sbjct: 518 -AGGRNVPLAVQRVAKASIEHSRKMKYQSLNEYRKRFLMKPYTSFEELTGEKEMAAGLEA 507
Query: 546 VYGEDVEELDILVGLMAEKKIKGFAISET 554
+YG D++ +++ L+ EK ET
Sbjct: 578 LYG-DIDAMELYPALLVEKPRPDAIFGET 507
BLAST of MS016603 vs. ExPASy TrEMBL
Match:
A0A6J1D8G8 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=1)
HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 635/638 (99.53%), Postives = 635/638 (99.53%), Query Frame = 0
Query: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR
Sbjct: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
Query: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT
Sbjct: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
Query: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE
Sbjct: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
Query: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD
Sbjct: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
Query: 241 GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED 300
GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED
Sbjct: 241 GKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDED 300
Query: 301 LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG 360
LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG
Sbjct: 301 LYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFG 360
Query: 361 GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN 420
GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN
Sbjct: 361 GLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGN 420
Query: 421 MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA 480
MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA
Sbjct: 421 MIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAA 480
Query: 481 LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM 540
LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM
Sbjct: 481 LEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLM 540
Query: 541 AEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIE 600
AEKKIKGFAISETAFVIFLLMASRRLEAD FFTSHFNEETYTKKGLEWVNTTESLKDVIE
Sbjct: 541 AEKKIKGFAISETAFVIFLLMASRRLEADTFFTSHFNEETYTKKGLEWVNTTESLKDVIE 600
Query: 601 RHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIPS 639
RHHPDI NKWMNSSSAFSVWDSPPNK NPIPIYLRIPS
Sbjct: 601 RHHPDIANKWMNSSSAFSVWDSPPNKRNPIPIYLRIPS 638
BLAST of MS016603 vs. ExPASy TrEMBL
Match:
A0A5A7UJ02 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00690 PE=4 SV=1)
HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 574/644 (89.13%), Postives = 608/644 (94.41%), Query Frame = 0
Query: 1 MLSQL-------LRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILG 60
MLSQL LR +LQKF+H+DFHE V+RMTVIDT LFLI+H VDKLG+WHKLPVILG
Sbjct: 1 MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60
Query: 61 LVYLAIRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRN 120
L+YLA+RRHLHQEYNLFNVGRTPVGVRFNPVD+PYRTADG YNDPFNE AGAQGSFFGRN
Sbjct: 61 LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120
Query: 121 ILPVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
I PVDQ+K LLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
Query: 181 ELVAPHEVATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLG 240
ELVAP EVA ++CPLKSFKFFKTKQV TGFFE KTGS+NVRTPWWDGSVLYGSN+EKLG
Sbjct: 181 ELVAPSEVA--SECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLG 240
Query: 241 KVRTYRDGKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKED 300
KVRTY+DGKLKIADD LLLHD+DGVAISGDVRNSWAGVSTLQALFIKEHNAVCD LKKED
Sbjct: 241 KVRTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKED 300
Query: 301 PELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGH 360
LEDEDLYRYARLVTSAVIAK+HTIDWTVELLK DTLLAGMRGNWYGLLGKKFKDTFGH
Sbjct: 301 ENLEDEDLYRYARLVTSAVIAKVHTIDWTVELLKMDTLLAGMRGNWYGLLGKKFKDTFGH 360
Query: 361 VGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLI 420
VGG+I GGLVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDFYLRDV++DP +KSPPL+
Sbjct: 361 VGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFYLRDVSVDPDHDKSPPLV 420
Query: 421 NKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRP 480
KVPM +MIGH GE SKKMGFTALLVSMGHQASGALELWNYPLWLRDL HD+DGKDRP
Sbjct: 421 EKVPMSDMIGHKGEVTSKKMGFTALLVSMGHQASGALELWNYPLWLRDLIAHDMDGKDRP 480
Query: 481 DHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEEL 540
DHVDLAALEVYRDRER+VARYNDFRRGL LIPISKWEDLTDD+EAIEVLR+VYG+DVEEL
Sbjct: 481 DHVDLAALEVYRDRERRVARYNDFRRGLFLIPISKWEDLTDDEEAIEVLREVYGDDVEEL 540
Query: 541 DILVGLMAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
DILVGLMAEKKIKGFAISETAFVIFL+MASRRLEADRFFTSHFNEETYTKKGLEWVNTTE
Sbjct: 541 DILVGLMAEKKIKGFAISETAFVIFLIMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
Query: 601 SLKDVIERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
SLKDVIERH+P+I+NKWMNSSSAFSVWDSPPNKPNPIPIYLRIP
Sbjct: 601 SLKDVIERHYPEISNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 642
BLAST of MS016603 vs. ExPASy TrEMBL
Match:
A0A1S3B8X6 (alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1)
HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 574/644 (89.13%), Postives = 608/644 (94.41%), Query Frame = 0
Query: 1 MLSQL-------LRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILG 60
MLSQL LR +LQKF+H+DFHE V+RMTVIDT LFLI+H VDKLG+WHKLPVILG
Sbjct: 1 MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60
Query: 61 LVYLAIRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRN 120
L+YLA+RRHLHQEYNLFNVGRTPVGVRFNPVD+PYRTADG YNDPFNE AGAQGSFFGRN
Sbjct: 61 LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120
Query: 121 ILPVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
I PVDQ+K LLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
Query: 181 ELVAPHEVATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLG 240
ELVAP EVA ++CPLKSFKFFKTKQV TGFFE KTGS+NVRTPWWDGSVLYGSN+EKLG
Sbjct: 181 ELVAPSEVA--SECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLG 240
Query: 241 KVRTYRDGKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKED 300
KVRTY+DGKLKIADD LLLHD+DGVAISGDVRNSWAGVSTLQALFIKEHNAVCD LKKED
Sbjct: 241 KVRTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKED 300
Query: 301 PELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGH 360
LEDEDLYRYARLVTSAVIAK+HTIDWTVELLK DTLLAGMRGNWYGLLGKKFKDTFGH
Sbjct: 301 ENLEDEDLYRYARLVTSAVIAKVHTIDWTVELLKMDTLLAGMRGNWYGLLGKKFKDTFGH 360
Query: 361 VGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLI 420
VGG+I GGLVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDFYLRDV++DP +KSPPL+
Sbjct: 361 VGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFYLRDVSVDPDHDKSPPLV 420
Query: 421 NKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRP 480
KVPM +MIGH GE SKKMGFTALLVSMGHQASGALELWNYPLWLRDL HD+DGKDRP
Sbjct: 421 EKVPMSDMIGHKGEVTSKKMGFTALLVSMGHQASGALELWNYPLWLRDLIAHDMDGKDRP 480
Query: 481 DHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEEL 540
DHVDLAALEVYRDRER+VARYNDFRRGL LIPISKWEDLTDD+EAIEVLR+VYG+DVEEL
Sbjct: 481 DHVDLAALEVYRDRERRVARYNDFRRGLFLIPISKWEDLTDDEEAIEVLREVYGDDVEEL 540
Query: 541 DILVGLMAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
DILVGLMAEKKIKGFAISETAFVIFL+MASRRLEADRFFTSHFNEETYTKKGLEWVNTTE
Sbjct: 541 DILVGLMAEKKIKGFAISETAFVIFLIMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
Query: 601 SLKDVIERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
SLKDVIERH+P+I+NKWMNSSSAFSVWDSPPNKPNPIPIYLRIP
Sbjct: 601 SLKDVIERHYPEISNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 642
BLAST of MS016603 vs. ExPASy TrEMBL
Match:
A0A0A0LG46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1)
HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 569/644 (88.35%), Postives = 607/644 (94.25%), Query Frame = 0
Query: 1 MLSQL-------LRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILG 60
MLSQL LR +LQKF+H+DFHE V+RMTVIDT LFLIVH VDKLG+WHKLPVILG
Sbjct: 1 MLSQLLKPIKIVLRGELQKFVHKDFHEVVQRMTVIDTFLFLIVHFVDKLGIWHKLPVILG 60
Query: 61 LVYLAIRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRN 120
L+YLA+RRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADG YNDPFNE AGAQGSFFGRN
Sbjct: 61 LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGKYNDPFNEGAGAQGSFFGRN 120
Query: 121 ILPVDQTKKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
I PVDQ+K LLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180
Query: 181 ELVAPHEVATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLG 240
ELVAP EVA ++CPLKSFKFFKTKQV TGFF+ KTGS+NVRTPWWDGSVLYGSN+EKLG
Sbjct: 181 ELVAPREVA--SECPLKSFKFFKTKQVSTGFFDNKTGSINVRTPWWDGSVLYGSNAEKLG 240
Query: 241 KVRTYRDGKLKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKED 300
KVRTY+DGKLKIADD LLLHD+DGVAISGDVRNSWAGVSTLQALFI+EHNAVCD LKKED
Sbjct: 241 KVRTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIREHNAVCDALKKED 300
Query: 301 PELEDEDLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGH 360
+LEDEDLYRYARLVTSAVIAK+HTIDWTVELLK DTLLAGMRGNWYGLLGKKFKDTFGH
Sbjct: 301 EDLEDEDLYRYARLVTSAVIAKVHTIDWTVELLKMDTLLAGMRGNWYGLLGKKFKDTFGH 360
Query: 361 VGGSIFGGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLI 420
VGG+I GGLVGLKKP+NHG+PYSLTEEFASVYRMHSLLPDDF+LRD+++DP NKSPPL+
Sbjct: 361 VGGAILGGLVGLKKPDNHGVPYSLTEEFASVYRMHSLLPDDFHLRDISVDPDHNKSPPLV 420
Query: 421 NKVPMGNMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRP 480
KVPM NMIGH GEE SKKMGFTALLVSMGHQ+SGALELWNYP WLRDL PHD+DGKDR
Sbjct: 421 EKVPMANMIGHKGEETSKKMGFTALLVSMGHQSSGALELWNYPQWLRDLIPHDMDGKDRA 480
Query: 481 DHVDLAALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEEL 540
DH+DLAALEVYRDRER+VARYNDFRRGL LIPISKWEDLTDD+ AIEVLR+VYG+DVEEL
Sbjct: 481 DHIDLAALEVYRDRERRVARYNDFRRGLFLIPISKWEDLTDDEGAIEVLREVYGDDVEEL 540
Query: 541 DILVGLMAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
DILVGLMAEKKIKGFAISETAFVIFL+MASRRLEADRFFTSHFNEETYTKKGLEWVNTTE
Sbjct: 541 DILVGLMAEKKIKGFAISETAFVIFLIMASRRLEADRFFTSHFNEETYTKKGLEWVNTTE 600
Query: 601 SLKDVIERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIP 638
SLKDVIERH+P+I+NKWMNSSSAFSVWDSPPNKPNPIPIY R+P
Sbjct: 601 SLKDVIERHYPEISNKWMNSSSAFSVWDSPPNKPNPIPIYFRLP 642
BLAST of MS016603 vs. ExPASy TrEMBL
Match:
A0A6J1DAJ0 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018381 PE=4 SV=1)
HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 561/614 (91.37%), Postives = 585/614 (95.28%), Query Frame = 0
Query: 25 MTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRRHLHQEYNLFNVGRTPVGVRFNPV 84
MTVIDTLLFLIVHL+DKLGVWHKLPVILGL+Y+AIRRH+HQEYNLFNVGRTPVGVRFN V
Sbjct: 1 MTVIDTLLFLIVHLIDKLGVWHKLPVILGLIYIAIRRHIHQEYNLFNVGRTPVGVRFNSV 60
Query: 85 DFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQTKKLLKPDPMVVATKLLARRKLIDT 144
DFPYRTA+G YNDPFNEA GAQGSFFGRNILP+DQTK+LLKP+PMVVATKLLAR+KLIDT
Sbjct: 61 DFPYRTANGKYNDPFNEAVGAQGSFFGRNILPIDQTKRLLKPNPMVVATKLLARKKLIDT 120
Query: 145 GKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEVATSNQCPLKSFKFFKTKQVPTGF 204
GKQFNMIA WIQFMIHDW+DHLEDTKQ+ELVAP EVA S++CPLKSFKFFKTKQVPTGF
Sbjct: 121 GKQFNMIAVXWIQFMIHDWMDHLEDTKQIELVAPREVAKSDECPLKSFKFFKTKQVPTGF 180
Query: 205 FEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLKIADDGLLLHDHDGVAISGDV 264
F+IKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLKIADDGLLLHDHDGVAISGDV
Sbjct: 181 FDIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLKIADDGLLLHDHDGVAISGDV 240
Query: 265 RNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDLYRYARLVTSAVIAKIHTIDWTVE 324
RNSWAGVSTLQALFIKEHNAVCD LKKED ++EDEDLYR ARLVTSAVIAK+HTIDW+ E
Sbjct: 241 RNSWAGVSTLQALFIKEHNAVCDALKKEDLDMEDEDLYRXARLVTSAVIAKVHTIDWSAE 300
Query: 325 LLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGLVGLKKPNNHGIPYSLTEEFASV 384
LLKT+TLLAG R NWYGLLGKKFKDTFGH GGS GG VGLKKPNNHGIPYSLTEEFASV
Sbjct: 301 LLKTETLLAGSRINWYGLLGKKFKDTFGHXGGSFLGGXVGLKKPNNHGIPYSLTEEFASV 360
Query: 385 YRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNMIGHNGEEASKKMGFTALLVSMGH 444
YRMHS LPD+FYLRDVT DPH NKSPPLINKVPMGNMIGH GEEASKKMGFTALLVSMGH
Sbjct: 361 YRMHSFLPDNFYLRDVTXDPHHNKSPPLINKVPMGNMIGHKGEEASKKMGFTALLVSMGH 420
Query: 445 QASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKVARYNDFRRGLLLI 504
QASGALELWNYPLWLRDL PHDVDGKDRP+HVDLAALEVYRDRERKVARYNDFRRGL LI
Sbjct: 421 QASGALELWNYPLWLRDLIPHDVDGKDRPNHVDLAALEVYRDRERKVARYNDFRRGLFLI 480
Query: 505 PISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGFAISETAFVIFLLMASR 564
PISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGF ISETAF IFLLMASR
Sbjct: 481 PISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGFLISETAFFIFLLMASR 540
Query: 565 RLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIERHHPDITNKWMNSSSAFSVWDSPP 624
RLEADRFFTSHFNE+TYTKKGLE VNTTESLKDVI RH+P I+NKWMNSSSAFSVWDS P
Sbjct: 541 RLEADRFFTSHFNEQTYTKKGLERVNTTESLKDVIRRHYPQISNKWMNSSSAFSVWDSSP 600
Query: 625 NKPNPIPIYLRIPS 639
NKPNPIPIYLRIPS
Sbjct: 601 NKPNPIPIYLRIPS 614
BLAST of MS016603 vs. TAIR 10
Match:
AT3G01420.1 (Peroxidase superfamily protein )
HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-293
Identity = 478/639 (74.80%), Postives = 549/639 (85.92%), Query Frame = 0
Query: 1 MLSQLLRSQLQKFIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIR 60
+++ L+ S L KFIH+DFHE RM+++D L LIVH VDK+ WHKLPV LGL YL +R
Sbjct: 3 VITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLEVR 62
Query: 61 RHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQT 120
RHLHQ+YNL NVG+TP G+RF+P ++PYRTADG +NDPFNE G+Q SFFGRN PVDQ
Sbjct: 63 RHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVDQK 122
Query: 121 KKLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHE 180
KL +PDPMVVATKLL R+K IDTGKQFNMIAASWIQFMIHDWIDHLEDT Q+ELVAP E
Sbjct: 123 SKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAPKE 182
Query: 181 VATSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRD 240
VA ++CPL SF+F KTK+VPTGFFEIKTGS N+RTPWWD SV+YGSNS+ L +VRTY+D
Sbjct: 183 VA--SKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKD 242
Query: 241 GKLKIADD-GLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDE 300
GKLKI+++ GLLLHD DG+AISGD+RNSWAGVS LQALFIKEHNAVCD LK ED +LEDE
Sbjct: 243 GKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDE 302
Query: 301 DLYRYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIF 360
DLYRYARLVTSAV+AKIHTIDWTV+LLKTDTLLAGMR NWYGLLGKKFKD+FGH G SI
Sbjct: 303 DLYRYARLVTSAVVAKIHTIDWTVQLLKTDTLLAGMRANWYGLLGKKFKDSFGHAGSSIL 362
Query: 361 GGLVGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMG 420
GG+VG+KKP NHG+PYSLTE+F SVYRMHSLLPD ++ D+ P NKS PLI ++ M
Sbjct: 363 GGVVGMKKPQNHGVPYSLTEDFTSVYRMHSLLPDQLHILDIDDVPGTNKSLPLIQEISMR 422
Query: 421 NMIGHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLA 480
++IG GEE +GFT L+VSMGHQASGALEL NYP+WLRD+ PHD +G+ RPDHVDLA
Sbjct: 423 DLIGRKGEETMSHIGFTKLMVSMGHQASGALELMNYPMWLRDIVPHDPNGQARPDHVDLA 482
Query: 481 ALEVYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGL 540
ALE+YRDRER V RYN+FRR + +IPI+KWEDLT+D+EAIEVL DVY DVEELD+LVGL
Sbjct: 483 ALEIYRDRERSVPRYNEFRRSMFMIPITKWEDLTEDEEAIEVLDDVYDGDVEELDLLVGL 542
Query: 541 MAEKKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVI 600
MAEKKIKGFAISETAF IFL+MA+RRLEADRFFTS FNE YTKKGLEWVNTTESLKDVI
Sbjct: 543 MAEKKIKGFAISETAFYIFLIMATRRLEADRFFTSDFNETIYTKKGLEWVNTTESLKDVI 602
Query: 601 ERHHPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLRIPS 639
+RH+PD+T+KWMNS SAFSVWDSPP NPIP+YLRIPS
Sbjct: 603 DRHYPDMTDKWMNSESAFSVWDSPPLTKNPIPLYLRIPS 639
BLAST of MS016603 vs. TAIR 10
Match:
AT1G73680.1 (alpha dioxygenase )
HSP 1 Score: 819.7 bits (2116), Expect = 1.6e-237
Identity = 395/624 (63.30%), Postives = 485/624 (77.72%), Query Frame = 0
Query: 13 FIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRRHLHQEYNLFNV 72
F+H H V +M+ D LF IVHLVDKLG+WH+ PV+LG+ YL +RRHLHQ YNL +V
Sbjct: 10 FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69
Query: 73 GRTPV-GVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQTKKLLKPDPMVV 132
G P+ G ++ +F YRTADG N P + G+QGSF GRN+ P +L P P VV
Sbjct: 70 G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVV 129
Query: 133 ATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEVATSNQCPLKS 192
ATKLLAR++ ID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA+ CPLKS
Sbjct: 130 ATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASG--CPLKS 189
Query: 193 FKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGKLKIADDGLL 252
FKF +TK+VPT K+G+VN RTPWWDGSV+YG++ + +VR ++DGKLKI+ DGLL
Sbjct: 190 FKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKISGDGLL 249
Query: 253 LHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDLYRYARLVTSA 312
D GV ISGD+RNSW+G S LQALF+KEHN+VCD LK+ P+ +DE LYR ARLVT+A
Sbjct: 250 ERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTARLVTAA 309
Query: 313 VIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGLVGLKKPNNH 372
VIAK+HTIDWT+ELLKTDTL AGMR NWYG GKK KD G G +F GLVGLKKPN+H
Sbjct: 310 VIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGPLFSGLVGLKKPNDH 369
Query: 373 GIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNMIGHNGEEASK 432
G+PYSLTEEF SVYRMH LLP+ LRD+ + ++P + ++PM +IG E +
Sbjct: 370 GVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIEREIPMTELIGKKAGEKAS 429
Query: 433 KMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALEVYRDRERKV 492
K+GF LLVSMGHQ+ GAL LWNYP W+R+L D+DG+DRP +D+AALE+YRDRER V
Sbjct: 430 KLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALEIYRDRERGV 489
Query: 493 ARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAEKKIKGFAIS 552
RYN+FR+ LL+ PISKWE+LTDD+EAI+VLR+VY +D+E+LD+ VGL AEKKIKGFAIS
Sbjct: 490 PRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAEKKIKGFAIS 549
Query: 553 ETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIERHHPDITNKWM 612
ETAF IFLL+ASRRLEADRFFT++FNE+TYTK+GLEWVNTTE+LKDVI+RH P +T++WM
Sbjct: 550 ETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTTETLKDVIDRHFPRLTDQWM 609
Query: 613 NSSSAFSVWDSPPNKPNPIPIYLR 636
SSAFSVW S PN N +P+YLR
Sbjct: 610 RCSSAFSVWGSDPNPKNWVPLYLR 628
BLAST of MS016603 vs. TAIR 10
Match:
AT1G73680.2 (alpha dioxygenase )
HSP 1 Score: 804.7 bits (2077), Expect = 5.5e-233
Identity = 393/633 (62.09%), Postives = 485/633 (76.62%), Query Frame = 0
Query: 13 FIHEDFHEAVKRMTVIDTLLFLIVHLVDKLGVWHKLPVILGLVYLAIRRHLHQEYNLFNV 72
F+H H V +M+ D LF IVHLVDKLG+WH+ PV+LG+ YL +RRHLHQ YNL +V
Sbjct: 10 FLHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHV 69
Query: 73 GRTPV-GVRFNPVDFPYRTADGNYNDPFNEAAGAQGSFFGRNILPVDQ-----TKKLLKP 132
G P+ G ++ +F YRTADG N P + G+QGSF GRN+ P + P
Sbjct: 70 G--PINGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGFFCQYFFFP 129
Query: 133 D----PMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQVELVAPHEVA 192
+ +VVATKLLAR++ ID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA
Sbjct: 130 ENLEKKIVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVA 189
Query: 193 TSNQCPLKSFKFFKTKQVPTGFFEIKTGSVNVRTPWWDGSVLYGSNSEKLGKVRTYRDGK 252
+ CPLKSFKF +TK+VPT K+G+VN RTPWWDGSV+YG++ + +VR ++DGK
Sbjct: 190 SG--CPLKSFKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGK 249
Query: 253 LKIADDGLLLHDHDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDTLKKEDPELEDEDLY 312
LKI+ DGLL D GV ISGD+RNSW+G S LQALF+KEHN+VCD LK+ P+ +DE LY
Sbjct: 250 LKISGDGLLERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLY 309
Query: 313 RYARLVTSAVIAKIHTIDWTVELLKTDTLLAGMRGNWYGLLGKKFKDTFGHVGGSIFGGL 372
R ARLVT+AVIAK+HTIDWT+ELLKTDTL AGMR NWYG GKK KD G G +F GL
Sbjct: 310 RTARLVTAAVIAKVHTIDWTIELLKTDTLTAGMRINWYGFFGKKVKDMVGARFGPLFSGL 369
Query: 373 VGLKKPNNHGIPYSLTEEFASVYRMHSLLPDDFYLRDVTIDPHRNKSPPLINKVPMGNMI 432
VGLKKPN+HG+PYSLTEEF SVYRMH LLP+ LRD+ + ++P + ++PM +I
Sbjct: 370 VGLKKPNDHGVPYSLTEEFVSVYRMHCLLPETLILRDMNSENVDKENPAIEREIPMTELI 429
Query: 433 GHNGEEASKKMGFTALLVSMGHQASGALELWNYPLWLRDLTPHDVDGKDRPDHVDLAALE 492
G E + K+GF LLVSMGHQ+ GAL LWNYP W+R+L D+DG+DRP +D+AALE
Sbjct: 430 GKKAGEKASKLGFEQLLVSMGHQSCGALTLWNYPNWMRNLVAQDIDGEDRPHLIDMAALE 489
Query: 493 VYRDRERKVARYNDFRRGLLLIPISKWEDLTDDDEAIEVLRDVYGEDVEELDILVGLMAE 552
+YRDRER V RYN+FR+ LL+ PISKWE+LTDD+EAI+VLR+VY +D+E+LD+ VGL AE
Sbjct: 490 IYRDRERGVPRYNEFRKNLLMSPISKWEELTDDEEAIKVLREVYEDDIEKLDLNVGLHAE 549
Query: 553 KKIKGFAISETAFVIFLLMASRRLEADRFFTSHFNEETYTKKGLEWVNTTESLKDVIERH 612
KKIKGFAISETAF IFLL+ASRRLEADRFFT++FNE+TYTK+GLEWVNTTE+LKDVI+RH
Sbjct: 550 KKIKGFAISETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTTETLKDVIDRH 609
Query: 613 HPDITNKWMNSSSAFSVWDSPPNKPNPIPIYLR 636
P +T++WM SSAFSVW S PN N +P+YLR
Sbjct: 610 FPRLTDQWMRCSSAFSVWGSDPNPKNWVPLYLR 637
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022150068.1 | 0.0e+00 | 99.53 | alpha-dioxygenase 1-like [Momordica charantia] | [more] |
| XP_038903382.1 | 0.0e+00 | 89.78 | alpha-dioxygenase 1-like isoform X1 [Benincasa hispida] | [more] |
| XP_038903383.1 | 0.0e+00 | 89.62 | alpha-dioxygenase 1-like isoform X2 [Benincasa hispida] | [more] |
| XP_008443484.1 | 0.0e+00 | 89.13 | PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 ... | [more] |
| XP_004147441.2 | 0.0e+00 | 88.35 | alpha-dioxygenase 1 [Cucumis sativus] >KGN59637.1 hypothetical protein Csa_00241... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SGH6 | 2.3e-292 | 74.80 | Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1 | [more] |
| Q9C9U3 | 2.3e-236 | 63.30 | Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1 | [more] |
| P27607 | 2.0e-14 | 22.43 | Prostaglandin G/H synthase 2 OS=Gallus gallus OX=9031 GN=PTGS2 PE=2 SV=1 | [more] |
| Q8HZR1 | 4.5e-14 | 22.47 | Prostaglandin G/H synthase 1 OS=Canis lupus familiaris OX=9615 GN=PTGS1 PE=1 SV=... | [more] |
| P70682 | 7.6e-14 | 22.94 | Prostaglandin G/H synthase 2 OS=Cavia porcellus OX=10141 GN=PTGS2 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D8G8 | 0.0e+00 | 99.53 | alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018335 PE=4 SV=... | [more] |
| A0A5A7UJ02 | 0.0e+00 | 89.13 | Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G... | [more] |
| A0A1S3B8X6 | 0.0e+00 | 89.13 | alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1 | [more] |
| A0A0A0LG46 | 0.0e+00 | 88.35 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1 | [more] |
| A0A6J1DAJ0 | 0.0e+00 | 91.37 | alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018381 PE=4 SV=... | [more] |
