MS016600 (gene) Bitter gourd (TR) v1

Overview


Name	MS016600
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold442: 80213 .. 81586 (-)
RNA-Seq Expression	MS016600
Synteny	MS016600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTTGACAAATGCATGGAAGGATGCATGTGCATCCGTTCGTCCTAGTGTAATAGTTATCCCGAGTGGGATATTTAAAGTAAGCGAAGGGAACTTTAAAGGCCCTTGTAAGAGCGCCATTGAGATTCGACTTCAAGGAACATTGGAAGCTCCACTCCATCCAAAGGGAGAGAGTTGGATTACTTTTGCATATATTGATCAATTGACGTTAATAGGTGAAGGTGTTTTCGATGGCCAAGGAGAAAATGGTTGGGAAAAGAATGATTGCCACAAAAACATAAATTGTGCTAAGCTTCCCATTGTAAGCTCTAAGCCTTTGTTTTTCTTTTTTTAATCTCATGCCGTCGTGTCCATTTTGAGAGGGCTTCGCAGATGTTCTCTAATATTTTAACTTTCACAATTTTGCATATTTCCTTAATTTTTAGAAAAATATTTAAATTTTAACCATGGAATTTGTTCTTAATATATAAGCTTTCTCTAAGCTTCTAACACGACATTTATCATATAAAAATGAGCAGAATTTAAGGTTCGACTTCATTACCAATTCAACCGTCAAGAGAATAACATCACTCGATAGTAAGAACTTCCATGTCAATGTTCTAGGCTGCAACAACCTCACATTTCATAGTGTCAAAATAATTGCACCCGAAAACAGTCCTAATACTGATGGAATACATATCGGACGATCAAAAGGGATCACAATCACAAAGTCACGTATCGCAACAGGAGATGATTGCATCTCCCTTGGAGATGGAAGCAAGCGAATAAAGGTCACCAATGTGACATGTGGGCCAGGACATGGCATCAGCATAGGCAGTCTTGGAAAATACACTAAAGAAGAACCTGTGGAAGGTGTCATAGTACAAAACTGCACCATAATAAACACCACTAATGGTGTGAGGATTAAAACATGGCCAGCTTCTCCAGCCACTGGCATAGCCACCAACATGCATTTTTTGGACATTATAATGATCAATGTTAGCAATCCTATTCTCATAGACCAAGAATATTGCCCATGGAATCAATGCAATCGTCAGGTATTTGATCACACTCCCAAATTTTTGAAAGAAAATATTTTTATCAGCTCTACAACAAAATCATTTTGATCCTATGGCATGTCATTCTAATTACTTTTCAGATTCCATCAAAGATAAAGTTAAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTCTGCAACTCCAGTAGCAGTCAAACTCATTTGTAGCAGTGGCCTGGGTTGTGGTGAGGTAAAAGTAGCTGATATTGACCTTACTTACAGTGGAATAGATGGTCCTGTTACATCCCAATGTTCAAATGTAAAACCCATCGTTACCGGAAAACAGAATCCTAAAATCTGCTCTTCCCCTATGTTT

mRNA sequence

GCTTTGACAAATGCATGGAAGGATGCATGTGCATCCGTTCGTCCTAGTGTAATAGTTATCCCGAGTGGGATATTTAAAGTAAGCGAAGGGAACTTTAAAGGCCCTTGTAAGAGCGCCATTGAGATTCGACTTCAAGGAACATTGGAAGCTCCACTCCATCCAAAGGGAGAGAGTTGGATTACTTTTGCATATATTGATCAATTGACGTTAATAGGTGAAGGTGTTTTCGATGGCCAAGGAGAAAATGGTTGGGAAAAGAATGATTGCCACAAAAACATAAATTGTGCTAAGCTTCCCATTAATTTAAGGTTCGACTTCATTACCAATTCAACCGTCAAGAGAATAACATCACTCGATAGTAAGAACTTCCATGTCAATGTTCTAGGCTGCAACAACCTCACATTTCATAGTGTCAAAATAATTGCACCCGAAAACAGTCCTAATACTGATGGAATACATATCGGACGATCAAAAGGGATCACAATCACAAAGTCACGTATCGCAACAGGAGATGATTGCATCTCCCTTGGAGATGGAAGCAAGCGAATAAAGGTCACCAATGTGACATGTGGGCCAGGACATGGCATCAGCATAGGCAGTCTTGGAAAATACACTAAAGAAGAACCTGTGGAAGGTGTCATAGTACAAAACTGCACCATAATAAACACCACTAATGGTGTGAGGATTAAAACATGGCCAGCTTCTCCAGCCACTGGCATAGCCACCAACATGCATTTTTTGGACATTATAATGATCAATGTTAGCAATCCTATTCTCATAGACCAAGAATATTGCCCATGGAATCAATGCAATCGTCAGATTCCATCAAAGATAAAGTTAAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTCTGCAACTCCAGTAGCAGTCAAACTCATTTGTAGCAGTGGCCTGGGTTGTGGTGAGGTAAAAGTAGCTGATATTGACCTTACTTACAGTGGAATAGATGGTCCTGTTACATCCCAATGTTCAAATGTAAAACCCATCGTTACCGGAAAACAGAATCCTAAAATCTGCTCTTCCCCTATGTTT

Coding sequence (CDS)

GCTTTGACAAATGCATGGAAGGATGCATGTGCATCCGTTCGTCCTAGTGTAATAGTTATCCCGAGTGGGATATTTAAAGTAAGCGAAGGGAACTTTAAAGGCCCTTGTAAGAGCGCCATTGAGATTCGACTTCAAGGAACATTGGAAGCTCCACTCCATCCAAAGGGAGAGAGTTGGATTACTTTTGCATATATTGATCAATTGACGTTAATAGGTGAAGGTGTTTTCGATGGCCAAGGAGAAAATGGTTGGGAAAAGAATGATTGCCACAAAAACATAAATTGTGCTAAGCTTCCCATTAATTTAAGGTTCGACTTCATTACCAATTCAACCGTCAAGAGAATAACATCACTCGATAGTAAGAACTTCCATGTCAATGTTCTAGGCTGCAACAACCTCACATTTCATAGTGTCAAAATAATTGCACCCGAAAACAGTCCTAATACTGATGGAATACATATCGGACGATCAAAAGGGATCACAATCACAAAGTCACGTATCGCAACAGGAGATGATTGCATCTCCCTTGGAGATGGAAGCAAGCGAATAAAGGTCACCAATGTGACATGTGGGCCAGGACATGGCATCAGCATAGGCAGTCTTGGAAAATACACTAAAGAAGAACCTGTGGAAGGTGTCATAGTACAAAACTGCACCATAATAAACACCACTAATGGTGTGAGGATTAAAACATGGCCAGCTTCTCCAGCCACTGGCATAGCCACCAACATGCATTTTTTGGACATTATAATGATCAATGTTAGCAATCCTATTCTCATAGACCAAGAATATTGCCCATGGAATCAATGCAATCGTCAGATTCCATCAAAGATAAAGTTAAGTAAAGTGAGCTTCAAGAACATTAGAGGAAGTTCTGCAACTCCAGTAGCAGTCAAACTCATTTGTAGCAGTGGCCTGGGTTGTGGTGAGGTAAAAGTAGCTGATATTGACCTTACTTACAGTGGAATAGATGGTCCTGTTACATCCCAATGTTCAAATGTAAAACCCATCGTTACCGGAAAACAGAATCCTAAAATCTGCTCTTCCCCTATGTTT

Protein sequence

ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSPMF

Homology

[Close]

BLAST of MS016600 vs. NCBI nr
Match: XP_022135905.1 (exopolygalacturonase-like [Momordica charantia])

HSP 1 Score: 652.1 bits (1681), Expect = 2.7e-183
Identity = 306/350 (87.43%), Postives = 333/350 (95.14%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL NAWKDACASVRPS +VIP GIFKVS+GNF+GPCKS IE RL+GTL+AP HPKGESWI
Sbjct: 49  ALANAWKDACASVRPSAVVIPRGIFKVSKGNFRGPCKSRIEFRLRGTLQAPTHPKGESWI 108

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAYID+LTL+G GVFDGQG+ GWEKNDCHK+INCAKLPI+LRFDFITNSTVKRITSLDS
Sbjct: 109 TFAYIDRLTLLGGGVFDGQGKAGWEKNDCHKSINCAKLPISLRFDFITNSTVKRITSLDS 168

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           K+FHVN+LGCNNLTFH V IIAPE+SPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS
Sbjct: 169 KHFHVNILGCNNLTFHGVNIIAPEDSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 228

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KRIKVTNVTCGPGHGISIGSLG+YTKEEPV+GVIV+NCTI+NTTNGVRIKTWPAS  TG 
Sbjct: 229 KRIKVTNVTCGPGHGISIGSLGRYTKEEPVKGVIVKNCTIMNTTNGVRIKTWPASADTGT 288

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHF+DIIM+NVS+PILIDQEYCPWNQCNRQIPSKIK+SKV+FKNIRGSSATPVAVKL
Sbjct: 289 ATNMHFVDIIMVNVSHPILIDQEYCPWNQCNRQIPSKIKISKVTFKNIRGSSATPVAVKL 348

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSP 351
           ICSSGL C EVK+ DIDLTY+GI GPVTSQCSNVKPI++GKQNP+ICSSP
Sbjct: 349 ICSSGLPCDEVKMTDIDLTYTGIQGPVTSQCSNVKPILSGKQNPRICSSP 398

[Close]

BLAST of MS016600 vs. NCBI nr
Match: XP_038887188.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 611.7 bits (1576), Expect = 4.0e-171
Identity = 287/350 (82.00%), Postives = 315/350 (90.00%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACASVRP  +VIP GIFKVSE  FKGPCKS IEIRL+G+L+AP HP G+SWI
Sbjct: 49  ALKDAWNDACASVRPCAVVIPKGIFKVSEAKFKGPCKSPIEIRLRGSLQAPKHPYGDSWI 108

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+++LTL G GVFDGQG  GWEKNDCHKNINCA LPI+LRFDFITNS VKRITSLDS
Sbjct: 109 TFAYVNRLTLSGGGVFDGQGRAGWEKNDCHKNINCATLPISLRFDFITNSIVKRITSLDS 168

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFH+NVLGCNNLTFH V IIAPENSPNTDGIHIGRS GI+ITKSRIATGDDCISLGDGS
Sbjct: 169 KNFHINVLGCNNLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGS 228

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+KVTNVTCGPGHGISIGSLGKYT EEPVEGVI++NCTI+NTTNGVRIKTWPASP +G 
Sbjct: 229 KRVKVTNVTCGPGHGISIGSLGKYTNEEPVEGVIIKNCTIMNTTNGVRIKTWPASPVSGT 288

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHF DIIM+NVSNPILIDQEYCPWNQCNR+IPSKIK+SKVSFKNIRGSSATP+AVKL
Sbjct: 289 ATNMHFSDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSSATPMAVKL 348

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSP 351
           ICS  L C EVKVA+IDL Y+G +GP+ S C NVKPI++G QNP+ CS P
Sbjct: 349 ICSRKLPCDEVKVANIDLVYNGKEGPIKSLCMNVKPIISGIQNPQTCSLP 398

[Close]

BLAST of MS016600 vs. NCBI nr
Match: XP_008455553.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo] >TYK21621.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 281/349 (80.52%), Postives = 317/349 (90.83%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS+RPS +VIP G FKV EG FKGPCKS +E+R++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK+I+CA+LPI+LRF+FITNS V+RITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHFLDI M+NVSNPILIDQEYCPWNQCNR+IPSKIK++KVSFKNIRGSSATPVAVKL
Sbjct: 290 ATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 350 ICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

[Close]

BLAST of MS016600 vs. NCBI nr
Match: KAA0059116.1 (exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 605.9 bits (1561), Expect = 2.2e-169
Identity = 282/349 (80.80%), Postives = 315/349 (90.26%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS+RPS +VIP G FKV EG FKGPCKS IE+R++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPIEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK I CA+LPI+LRF+FITNS V+RITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKRIGCARLPISLRFNFITNSIVRRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHFLDI M+NVSNPILIDQEYCPWNQCNR+IPSKIK++KVSFKNIRGSSATPVAVKL
Sbjct: 290 ATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 350 ICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

[Close]

BLAST of MS016600 vs. NCBI nr
Match: XP_031742281.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 605.5 bits (1560), Expect = 2.9e-169
Identity = 284/349 (81.38%), Postives = 314/349 (89.97%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACASVRPS +VIP GIFKV EG FKGPCKS IEIR++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASVRPSAVVIPKGIFKVREGEFKGPCKSPIEIRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK I+CA LPI+LRFDFITNS VKRITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKRIDCANLPISLRFDFITNSIVKRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KS+IATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFKGVNIIAPEDSPNTDGIHIGRSIGISILKSQIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPNAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           AT+MHF DI M+NVSNPILIDQEYCPWNQCNR+IPS IK++ VSFKNIRGSSATPVAVKL
Sbjct: 290 ATDMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINNVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS   C EVKVA+IDL Y+GI+GP+TSQC NVKPIV+G QNP ICSS
Sbjct: 350 ICSSNQPCEEVKVANIDLVYNGINGPITSQCMNVKPIVSGIQNPSICSS 398

[Close]

BLAST of MS016600 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.1e-122
Identity = 213/352 (60.51%), Postives = 255/352 (72.44%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHP---KGE 60
           ALT AWK+ACAS  PS I++P G F V     +GPCKS+I ++LQGTL+AP  P   KG 
Sbjct: 5   ALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGL 64

Query: 61  SWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKN-INCAKLPINLRFDFITNSTVKRIT 120
            WI    ID LT+ G GVFDGQG++ W +NDCHKN   C  L +NLR   +TNS ++ +T
Sbjct: 65  GWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDVT 124

Query: 121 SLDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISL 180
           +LDSKNFHVNV+GC NLTF   KI A E S NTDGIHIGRS G+ I  + I TGDDCISL
Sbjct: 125 TLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCISL 184

Query: 181 GDGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASP 240
           GDGSK I +TN+TCGPGHGIS+GSLG+Y  EE V G+ V+NCTI  + NGVRIKTWP S 
Sbjct: 185 GDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKS- 244

Query: 241 ATGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPV 300
             G A+ MHF DI M +V  PILIDQ YCP+NQC  ++PS +KLSK+SFKNI+G+S T  
Sbjct: 245 EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKE 304

Query: 301 AVKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICS 349
           AVKL+CS    C  V++ADIDLTYSG  GP TS C N+KP + GKQ P ICS
Sbjct: 305 AVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICS 355

[Close]

BLAST of MS016600 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.0e-118
Identity = 195/351 (55.56%), Postives = 255/351 (72.65%), Query Frame = 0


Query: 4   NAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHP---KGESWI 63
           +AWK+ACASV PS +VIP G + +S+ N +GPCK+ IEI +QGT++AP  P   K  +W+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 64  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 123
            F  ++   + G G+FDGQG   +EKN C      +KLP+N+RFDF+TN+ ++ ITS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDS 170

Query: 124 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 183
           K FH+NV  C N+T   +KI AP+ SPNTDGIH+G+S+G+ I  S I TGDDCIS+GDG+
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 184 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 243
           K + +  +TCGPGHGISIGSLGK+  EEPVEG+ + NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHG-GA 290

Query: 244 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 303
            + +HF DI M NVS+PILIDQ+YCPWN+C +   SK+KLS +SFKNIRG+SA P A+K 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 304 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSPM 352
           ICS    C  V++ADID+ ++G + P TSQC NVKPI +GK NP  CS P+
Sbjct: 351 ICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVKPITSGKLNPIPCSGPV 399

[Close]

BLAST of MS016600 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 9.1e-118
Identity = 195/351 (55.56%), Postives = 254/351 (72.36%), Query Frame = 0


Query: 4   NAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHP---KGESWI 63
           +AWK+ACASV PS +VIP G + +S+ N +GPCK+ IEI +QGT++AP  P   K  +W+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 64  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 123
            F  ++   + G G+FDGQG   +EKN C      +KLP+N+RFDF+TN+ ++ ITS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDS 170

Query: 124 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 183
           K FH+NV  C N+T   +KI AP+ SPNTDGIH+G+S+G+ I  S I TGDDCIS+GDG+
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 184 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 243
           K + +  +TCGPGHGISIGSLGK+  EEPVEG+ + NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHG-GA 290

Query: 244 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 303
            + +HF DI M NVS+PILIDQ+YCPWN+C +   SK+KLS +SFKNIRG+SA P A+K 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 304 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSPM 352
           ICS    C  V++ADID+ ++G + P TSQC NVKPI  GK NP  CS P+
Sbjct: 351 ICSGSSPCQNVELADIDIQHNGAE-PATSQCLNVKPITIGKLNPIPCSGPV 399

[Close]

BLAST of MS016600 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.5e-112
Identity = 192/352 (54.55%), Postives = 254/352 (72.16%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHP---KGE 60
           AL NA+K+AC S  PS IVIP G F +++   +GPCKS +E+++Q TL+AP  P   K  
Sbjct: 43  ALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVG 102

Query: 61  SWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITS 120
            W+T   +DQ T+ G G+ DGQ    WE   C ++  C KLP NL F+ +TNST+K IT+
Sbjct: 103 EWLTVNKLDQFTMSGGGILDGQAAAAWE---CKQSKKCNKLPNNLSFNSLTNSTIKDITT 162

Query: 121 LDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLG 180
           LDSK+FHVNV  C NLTF    + AP NSPNTDGIH+ RS  + IT S  +TGDDCIS+G
Sbjct: 163 LDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVG 222

Query: 181 DGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPA 240
           D ++++ +T VTCGPGHGIS+GSLG    E+PV GV V+NCT  NT NGVRIKTWPAS  
Sbjct: 223 DETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPAS-H 282

Query: 241 TGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVA 300
            G+  ++HF DII+ NVSNP++IDQ YCP+N+CN+ +PS++K+SKVSF+NI+G+S T  A
Sbjct: 283 PGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDA 342

Query: 301 VKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           V L+ S G+ C  ++V DID+TYSG +GP  S C N+KP + GKQNP +C++
Sbjct: 343 VSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVCTA 390

[Close]

BLAST of MS016600 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 8.3e-111
Identity = 187/350 (53.43%), Postives = 246/350 (70.29%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCK-SAIEIRLQGTLEAPLHP---KG 60
           A+  AWK ACAS  PS ++IP G + + E   +GPCK S I  ++ G ++AP  P   K 
Sbjct: 26  AVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKFKS 85

Query: 61  ESWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRIT 120
           + W++F  ID LT+ G G  DGQG+  W KN+C KN NC    +NLRFDF+ ++ V+ IT
Sbjct: 86  DGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRDIT 145

Query: 121 SLDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISL 180
           SL+SK FH+NVL C ++TF  V + AP  S NTDGIH+G SKG+TIT ++IATGDDCIS+
Sbjct: 146 SLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISI 205

Query: 181 GDGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASP 240
           G GS+ + +T V CGPGHGISIGSLG+Y  E+ V G+ V+ CT   T NGVR+KTWP SP
Sbjct: 206 GPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSP 265

Query: 241 ATGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPV 300
             G AT++ F D+ M NV NP+++DQEYCP+ QC+RQ PS+IKLS ++F NIRG+S   V
Sbjct: 266 -PGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKV 325

Query: 301 AVKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKI 347
           AV + CS G+ C  +K+ +I+L+Y G  GP TS CSNVKP  +GKQ P I
Sbjct: 326 AVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVPAI 374

[Close]

BLAST of MS016600 vs. ExPASy TrEMBL
Match: A0A6J1C2C8 (exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007743 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 306/350 (87.43%), Postives = 333/350 (95.14%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL NAWKDACASVRPS +VIP GIFKVS+GNF+GPCKS IE RL+GTL+AP HPKGESWI
Sbjct: 49  ALANAWKDACASVRPSAVVIPRGIFKVSKGNFRGPCKSRIEFRLRGTLQAPTHPKGESWI 108

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAYID+LTL+G GVFDGQG+ GWEKNDCHK+INCAKLPI+LRFDFITNSTVKRITSLDS
Sbjct: 109 TFAYIDRLTLLGGGVFDGQGKAGWEKNDCHKSINCAKLPISLRFDFITNSTVKRITSLDS 168

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           K+FHVN+LGCNNLTFH V IIAPE+SPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS
Sbjct: 169 KHFHVNILGCNNLTFHGVNIIAPEDSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 228

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KRIKVTNVTCGPGHGISIGSLG+YTKEEPV+GVIV+NCTI+NTTNGVRIKTWPAS  TG 
Sbjct: 229 KRIKVTNVTCGPGHGISIGSLGRYTKEEPVKGVIVKNCTIMNTTNGVRIKTWPASADTGT 288

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHF+DIIM+NVS+PILIDQEYCPWNQCNRQIPSKIK+SKV+FKNIRGSSATPVAVKL
Sbjct: 289 ATNMHFVDIIMVNVSHPILIDQEYCPWNQCNRQIPSKIKISKVTFKNIRGSSATPVAVKL 348

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSP 351
           ICSSGL C EVK+ DIDLTY+GI GPVTSQCSNVKPI++GKQNP+ICSSP
Sbjct: 349 ICSSGLPCDEVKMTDIDLTYTGIQGPVTSQCSNVKPILSGKQNPRICSSP 398

[Close]

BLAST of MS016600 vs. ExPASy TrEMBL
Match: A0A5D3DD75 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00190 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.2e-170
Identity = 281/349 (80.52%), Postives = 317/349 (90.83%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS+RPS +VIP G FKV EG FKGPCKS +E+R++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK+I+CA+LPI+LRF+FITNS V+RITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHFLDI M+NVSNPILIDQEYCPWNQCNR+IPSKIK++KVSFKNIRGSSATPVAVKL
Sbjct: 290 ATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 350 ICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

[Close]

BLAST of MS016600 vs. ExPASy TrEMBL
Match: A0A1S3C2F4 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103495704 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.2e-170
Identity = 281/349 (80.52%), Postives = 317/349 (90.83%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS+RPS +VIP G FKV EG FKGPCKS +E+R++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK+I+CA+LPI+LRF+FITNS V+RITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHFLDI M+NVSNPILIDQEYCPWNQCNR+IPSKIK++KVSFKNIRGSSATPVAVKL
Sbjct: 290 ATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 350 ICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

[Close]

BLAST of MS016600 vs. ExPASy TrEMBL
Match: A0A5A7UY44 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00230 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 282/349 (80.80%), Postives = 315/349 (90.26%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS+RPS +VIP G FKV EG FKGPCKS IE+R++GTL+AP HP G+SWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPIEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++TL G GVFDGQG+ GWEKNDCHK I CA+LPI+LRF+FITNS V+RITSLDS
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKRIGCARLPISLRFNFITNSIVRRITSLDS 169

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPE+SPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 170 KNFHVNILGCNNLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 229

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KR+K+TNVTCGPGHGISIGSLGKYT EEPVEGVIV+NCTI+NTTNGVRIKTWPASP  GI
Sbjct: 230 KRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNGVRIKTWPASPVAGI 289

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHFLDI M+NVSNPILIDQEYCPWNQCNR+IPSKIK++KVSFKNIRGSSATPVAVKL
Sbjct: 290 ATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFKNIRGSSATPVAVKL 349

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 350 ICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPICSS 398

[Close]

BLAST of MS016600 vs. ExPASy TrEMBL
Match: A0A0A0K3B7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.9e-167
Identity = 282/349 (80.80%), Postives = 306/349 (87.68%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKGESWI 60
           AL +AW DACAS RPS +VIP G FKV EG FKGPCKS IE R+ GTL+AP HP G+SWI
Sbjct: 49  ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 108

Query: 61  TFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLDS 120
           TFAY+D++ L G GVFDGQG+ GWEKNDCHK I+CAKLPI+LRFDFITNS V+RITSLDS
Sbjct: 109 TFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDS 168

Query: 121 KNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDGS 180
           KNFHVN+LGCNNLTF  V IIAPENSPNTDGIHIGRS GI+I KSRIATGDDCISLGDGS
Sbjct: 169 KNFHVNILGCNNLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGS 228

Query: 181 KRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATGI 240
           KRIKV NV CGPGHGISIGSLGKY  EEPVEGVIV NCTIINTTNGVRIKTWPASP  GI
Sbjct: 229 KRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGI 288

Query: 241 ATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVKL 300
           ATNMHF DI M+NVSNPILIDQEYCPWNQCNR+IPS IK++KVSFKNIRGSSATPVAVKL
Sbjct: 289 ATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKL 348

Query: 301 ICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           ICSS L C EVKVA+IDL Y+GI G +TS+C NVKPI++G QNP ICSS
Sbjct: 349 ICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSS 397

[Close]

BLAST of MS016600 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 3.4e-120
Identity = 198/352 (56.25%), Postives = 254/352 (72.16%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLH--PKGES 60
           AL  A+  AC S  PS +VIP G FK+ E   +GPCK+ IE+ LQGT++A  +     E 
Sbjct: 38  ALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEK 97

Query: 61  WITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSL 120
           W+ F  ID   L G G FDG+G   W  N+CHK   C KLPI++RFDFI NS ++ I+S+
Sbjct: 98  WVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSI 157

Query: 121 DSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGD 180
           D+KNFH+NVLG  N+T +++KI+APE+SPNTDGIH+GRS G+ I  S I+TGDDCIS+GD
Sbjct: 158 DAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGD 217

Query: 181 GSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPAT 240
           G K + V  VTCGPGHGIS+GSLG+Y  E+ V G+ V NCT+  T NG+RIKTWP++  +
Sbjct: 218 GMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACS 277

Query: 241 GIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAV 300
             A+++HF DII+ +VSNPILIDQEYCPWNQCN+Q  S IKL  +SFKNIRG+S    AV
Sbjct: 278 TTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAV 337

Query: 301 KLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSP 351
           KL+CS G  C  V++ DID+ Y+G DGP T  CSNV P + G Q+PK CS+P
Sbjct: 338 KLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPKACSAP 389

[Close]

BLAST of MS016600 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-118
Identity = 197/352 (55.97%), Postives = 254/352 (72.16%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPKG-ESW 60
           AL  A+ +AC     S ++IP G +K+ E    GPCK+ I I L GT++A  +  G E W
Sbjct: 39  ALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKW 98

Query: 61  ITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITSLD 120
           + F  I+   L G GVFDG+G   W  N+CHK  NC KLPI++RFDF+T++ ++ ITSLD
Sbjct: 99  VAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKIRGITSLD 158

Query: 121 SKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLGDG 180
           +K+FH+NV+G  N+TF  VKIIAP  SPNTDGIH+GRS GI I  S I+TGDDC+S+GDG
Sbjct: 159 AKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDG 218

Query: 181 SKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPATG 240
            K + V  VTCGPGHGISIGSLG+Y+ EE V G+ + NCT+  T NG+RIKTWP++  T 
Sbjct: 219 MKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTT 278

Query: 241 IATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVAVK 300
            A+++HF +I++ NVSNPILIDQEYCPWNQCN+Q PS IKL+ +SFK IRG+S    AVK
Sbjct: 279 TASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVK 338

Query: 301 LICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSPM 352
           L+CS G  C  V+V D+++ Y+G DGP T QCSNV P + G Q PK CSSP+
Sbjct: 339 LLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACSSPV 390

[Close]

BLAST of MS016600 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 6.1e-117
Identity = 194/353 (54.96%), Postives = 252/353 (71.39%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEA---PLHPKGE 60
           AL  A+  AC S  P  +VIP G FK+ E    GPCKS +EI L GT+ A    +H K E
Sbjct: 39  ALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSIHGK-E 98

Query: 61  SWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITS 120
            W+ F  +D   L G G FDG+G   W  N+CHK   C KLPI++RFDF+TN+ ++ I+S
Sbjct: 99  KWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISS 158

Query: 121 LDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLG 180
           +D+KNFH+NV+G  N+TF +VKI+AP  SPNTDGIH+GRS G++I  SRI+TGDDC+S+G
Sbjct: 159 IDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVG 218

Query: 181 DGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPA 240
           DG   + V NV CGPGHGIS+GSLG+Y  E+ V G+ V NCT+  T NG+RIKTWP++  
Sbjct: 219 DGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAAC 278

Query: 241 TGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVA 300
           +  A+N+HF +II+ NVSNPILIDQEYCPWNQCN+Q  S IKL+ +SF+ IRG+S    A
Sbjct: 279 STTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDA 338

Query: 301 VKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSSP 351
           VKL+CS G  C  V+V DI++ Y+G DGP T  CSNV+P + G Q PK C++P
Sbjct: 339 VKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQFPKACNTP 390

[Close]

BLAST of MS016600 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.0e-116
Identity = 195/352 (55.40%), Postives = 249/352 (70.74%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEA---PLHPKGE 60
           AL  A+  AC +   S +VI  G FK+ E    GPCK+ +EI LQGTL+A    +  K E
Sbjct: 39  ALLKAFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGK-E 98

Query: 61  SWITFAYIDQLTLIGEGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRITS 120
            W+ F  I+   L G G+FDG+G   W  N+CHK   C KLPI++RFDF+ N+ ++ I+S
Sbjct: 99  RWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISS 158

Query: 121 LDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCISLG 180
           +D+KNFH+NVLG  N+TF +VK+IAP  SPNTDGIH+GRS+G+ I  S+IATGDDCIS+G
Sbjct: 159 IDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVG 218

Query: 181 DGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPASPA 240
           DG K + V NV CGPGHGIS+GSLG+Y  E+ V G+ V NCT+  T NG+RIKTWP++  
Sbjct: 219 DGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAAC 278

Query: 241 TGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATPVA 300
              A+ +HF +II+ NVSNPILIDQEYCPWNQCN+Q PS IKL  +SFKNIRG+S    A
Sbjct: 279 ATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDA 338

Query: 301 VKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICSS 350
           VKL+CS    C  V++ +I+L Y G DGP T  CSNV P + G QNPK CS+
Sbjct: 339 VKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPKACSA 389

[Close]

BLAST of MS016600 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.1e-105
Identity = 185/353 (52.41%), Postives = 239/353 (67.71%), Query Frame = 0


Query: 1   ALTNAWKDACASVRPSVIVIPSGIFKVSEGNFKGPCKSAIEIRLQGTLEAPLHPK----G 60
           A   AWK+ACA+   S I +P G + V    FKGPCK  + + L G  +AP   K     
Sbjct: 89  AFAAAWKEACAA--GSTITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 148

Query: 61  ESWITFAYIDQLTLIG-EGVFDGQGENGWEKNDCHKNINCAKLPINLRFDFITNSTVKRI 120
             WI F  +   TL G + +FDGQG   W+ NDC K   C  LPIN+RF  +TNS +  I
Sbjct: 149 AGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSI 208

Query: 121 TSLDSKNFHVNVLGCNNLTFHSVKIIAPENSPNTDGIHIGRSKGITITKSRIATGDDCIS 180
           TS +SK FH+N+L C N+T   + I AP  S NTDGIHIGRS G+ +  ++I TGDDC+S
Sbjct: 209 TSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVS 268

Query: 181 LGDGSKRIKVTNVTCGPGHGISIGSLGKYTKEEPVEGVIVQNCTIINTTNGVRIKTWPAS 240
           +GDG++ + V NV CGPGHGISIGSLG+Y  E+PV+GV V+ C I NT NGVRIKTWP S
Sbjct: 269 IGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS 328

Query: 241 PATGIATNMHFLDIIMINVSNPILIDQEYCPWNQCNRQIPSKIKLSKVSFKNIRGSSATP 300
           P  GIA+N+ F DI M NVS P+LIDQEYCP+  C   +PSK+KLS V+ KNI+G+SAT 
Sbjct: 329 P-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATK 388

Query: 301 VAVKLICSSGLGCGEVKVADIDLTYSGIDGPVTSQCSNVKPIVTGKQNPKICS 349
           VAVKL+CS G+ C  + ++DI+L ++G +GP  S CSN+KPI++GK  P  C+
Sbjct: 389 VAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAACT 438

The following BLAST results are available for this feature:

BLAST of MS016600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135905.1	2.7e-183	87.43	exopolygalacturonase-like [Momordica charantia]	[more]
XP_038887188.1	4.0e-171	82.00	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_008455553.1	1.7e-169	80.52	PREDICTED: exopolygalacturonase-like [Cucumis melo] >TYK21621.1 exopolygalacturo...	[more]
KAA0059116.1	2.2e-169	80.80	exopolygalacturonase-like [Cucumis melo var. makuwa]	[more]
XP_031742281.1	2.9e-169	81.38	exopolygalacturonase-like [Cucumis sativus]	[more]

BLAST of MS016600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	2.1e-122	60.51	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q39786	7.0e-118	55.56	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
Q39766	9.1e-118	55.56	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q05967	1.5e-112	54.55	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
Q6H9K0	8.3e-111	53.43	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]

BLAST of MS016600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2C8	1.3e-183	87.43	exopolygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111007743 PE=3 SV...	[more]
A0A5D3DD75	8.2e-170	80.52	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C2F4	8.2e-170	80.52	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103495704 PE=3 SV=1	[more]
A0A5A7UY44	1.1e-169	80.80	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A0A0K3B7	2.9e-167	80.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037910 PE=3 SV=1	[more]

BLAST of MS016600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07820.1	3.4e-120	56.25	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	1.1e-118	55.97	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	6.1e-117	54.96	Pectin lyase-like superfamily protein	[more]
AT3G07840.1	1.0e-116	55.40	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	1.1e-105	52.41	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 130..156 e-value: 320.0 score: 10.1 coord: 157..178 e-value: 140.0 score: 11.3 coord: 210..231 e-value: 0.57 score: 19.2 coord: 180..200 e-value: 3000.0 score: 3.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 10..335 e-value: 5.7E-101 score: 337.8
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 187..200
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..339 e-value: 2.0E-97 score: 328.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..349
None	No IPR available	PANTHER	PTHR31375:SF248	POLYGALACTURONASE	coord: 2..349
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 1..348

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016600.1	MS016600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity