MS016514 (gene) Bitter gourd (TR) v1

Overview


Name	MS016514
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold657: 358074 .. 362916 (-)
RNA-Seq Expression	MS016514
Synteny	MS016514

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATGGGTGGGAAGAATTTGAAATGTCTTACATCTGTTGCTTTCATTTGTGTTTTCGTTTGGTGTTTTAGTTTGCAAAATTGCCATGCAAGAGAAGGCAGGCATTTGACACACACAAAAACTGGGTCAGGTAGAGAGATTGAGAAGAAAGTTAGAGATGACACTGGTTTTTCTGGCCGCCACCGCCACGTTCATCGCCACCGTGGAGGTAGCGGCCCGTCGAGGTCCACAAGCGGTGGCCGTGTCGGAAGTGAGAATATTGTGGGAGTCTCACTAGCAAGTAAAGGTGGTTCTTCCAACTTTAATGTGTTGGACTTTGGAGCTAAGGGTGATGGAAGGGCAGATGACACCAAGGTGAATTTAGGTTGACATTTACTTTATCATTTTCTATCATTTTCCACGACGTGATTCTCAATTTGGCTCTTGATCTTTTAAAATTTTCGATTTTATCTTTAATGTATTAAAATTTCTTAATTATGTCTTTCTATAGAAAAATCAATTCAAATTTGTTAATAAAAGGTTGATGTGCTAATAAATTATTTGTTTTATTAACATATTTGAACAGGTAGCAATGCCACTAGATTTAGATTTTGTTAATAGGCGATTGTGTTTTGTGTGTCAAGTCGAACCTAAATGGTATGTTCATGTGTTCAAATATATTAAAGATCCATCAACTTTTTGTTAACAATTATTTTATGGAATGATTTAATTGAGAATTTTTCACAAGTGAAATTGAGAATTTTAAAAGATAAAGATTACACAAAGACTTGTACATATGTAGCCTAGAATTTTCATTACATATAAGTTGCACAAGCATTTATACTTTACAGAAATGAAAACTCACAGAAGTTCTTGACAATGTATGCATAGCAGGCATTTCAGGCAGCTTGGGAAGCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCGCTGGCTCCGAATTCCTCGTTGGCCCCATCTCGTTCTCGGGTCCTAATTGCCAACCGAACATTGTATTTCAGGTCAAAATCAATAACTGCTTAAGTGCAAAATAAGACCGGCCCCATTTTATAACCATTTTGTTGTTTTTTGATAAGAAACCGAACTTTTTCATTGAGGCAAATGAAAACAGAAACATATATACAAGGGCATACAAAAACAAAAGTCTAGAAGGAGGAGCCCTACCTAAAGAAAAGAGCTCCCAATCCAAAAGAATAAAATCTAACTATTTGTTTTTTATTATCTGTTTTTTAACATTAAGCTTGTTAACTCACGATTCCTTTGGTATGTTTTAACATTTTTTTAGAAACATATTTGAACTCTTAGGCAAATTAAAAATAAATAAACACTTTTGGAGGCTACTTTTTTAGTTTTCAAATTTTAGCTTAGATTTTGAAAATATTTTTAAAAAGTAGATGCAAAACAAAGAAATACATAGATGAAAATAGTGTTTAAAAGCTAAAATAGAATAATTAACAAACGGGCTATAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCTACAAAGAACTTTCCAAAAAAGCTCCTTTGTTTGCAGATAAAAAATTTCTTGATAACATATTTTTAAGCACATCTCCTATGTTGACATGCAGTTGGATGGCAAAATCATTGCTCCAACAAGCTCAAGTGCTTGGGGTTCAGGTCTCTTACAGTGGATTGAGTTCACTAAACTTAAAGGGATTACAGTGAAGGGTTCAGGAACTATTGATGGACAAGGCTCGGCTTGGTGGAACGACTCACCGACATACAATCCTACGGATGAAAATTTGGTAATTTCTTAAAGAGTCAAATACATTTTTGATCCCTGAAGTTTGGACTTAGTTTCTATTTAGTAACTAATTTTTAAATTTTGTTTCCATTCGGACCTTGAAGTTCTAAAAGTGACACGTTTGGTCCCTGAAGTTTGAATTTAGTTTTTACTTGATCCCTAAGGTTTGAATTTGGTTTCTAATTGGTTCTTGAGTTGAGTTTATCTTTAGTTGACATAACGGAATGACGATGTGGTAGCTAACCGCATAATTGGTCCTTGAGATCTCTCGTTGTCTATTCATCATAAGATAACTACCACATCATCATTCTATTAGGTCAACTAACGGTAACTGCACTCATTAACCAATTAGAAACCAAAATCAAACCTCATGGACCAATTAAATATTAAATTCAAACTACAGAGATCAAACATGTCACAATTGAACTTAAGCACAAAATAGAAACTAAATTTATTCGAAAGACCAAAAGTGATGGTGTTTTAGACTCACTGACCAAATAGAAACCAAGTAAAATGTTTATTTAAAGTGAAACAGCTATCTAATCACTGTTAAATCACCAATCAACCCAAAAGTTTAAAATTGATAGGTCACAGTAAATTTAATCTTATAGATGGCTTTAATCTCATGCAGGACGAGCAAAGCAGTAGCGGAAAACTTCCAAGCACCAAACCAACAGTACAGTCTTGGCTTAAACTTGAGAATAGTAGAAAAAATGGGTCAAAGTTATCTGTTTGTAAATACTCTGATATTTTTGTGCAGGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATTCAAAATAGCCAACAGACTCACCTGAAATTTGATGGTTGCACATCGGTTCAGGTCTTTTCCCTAACCGTTTCATCGCCCGGTGACAGCCCGAACACAGATGGAATTCACTTGCAGAACTCTCAGAACGTGATCATCTCAAACAGCAATCTAGCTTGTGGTAATCCTCAATTTTGAGCACTAACTGCAAAAATAGCTTTCTTTAGCATATGAGAGTTTAACTTTTGAAAAGTATTCCAACTTAATATAAGCAATTGTTTAGAATTCAGAAACCAAGCTTTCATAGAAAATGGGAGTATATACAAGTAGCATAAAAAAAACAAGTCCACCAAAATGGGATCCCCTATAAGAAGGGATTCTAGTCCAAAATAATGACGCACAAAAAGTCTTAGAAACCGACACCCATACAGAGGTATTAAATCTAACCAAATCTCAAACTTCCCCCGAGACCTCCCCACTCCCTAAAAATTCTACTATTCATCTCAGTCCAAATATCCCACAAAATAGCAAAAAACAAGTCTGCTACAATGCATCCTCTATACTGAAAAGAGGCCAAGCACAACCTCCTCCACCATCATCCTACGGTCCCTATTACAAGCCAATGACAACCCAAAAACTTTTGGGAACTAGCCCCAAACAGATTGAACAAACTGGCAACTCCATAGGATACGGTTGAGGCCCTCAAAAGCATTTTTGCAAAGCACACTATTGGGGACCCAACAAAAAAGAATACGCTCTATGGTATTAGCTTTCACTTTTGAAGGAAGATCAACTTCATTTTGGGAAAGCAACTCATCCGTGAAAACAAGTTTGAAAGAACCATTTAACCGAACATATATTCACTAGTAAATAAAAAATAAAAAAATAAAAACTCTTAGATTCAACTACCAAATAGAATCTTATTCCCTTAAAAACTAATCTGATGTGAACATGAAACTTACATAAATGAAAATCAAACTACAGGAGACGATTGTATATCCATACAAACTGGAAGCTCAGGCATATACATACACAACATCAACTGTGGACCAGGCCACGGAATTAGCATTGGAGGGCTAGGACGCGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTTCGCGACATCAAACTACGGAACACAATGACTGGTGTCAGAATAAAAACATGGCAGGTATATATAACTTTTCTTCTCTAATTTTAACTCCAAGTTGGTAATAAATGTATGCCAAGTAAAACCAGAGGCATTGATTGTAATCTTGCCCCACTTTTAATTTTTCATAAGAAACCAGCCTTCAAGGAGAGAAACGAAAAAATGTACCAGGACAAAAAAAAAAAAATAGCACGATAAAAGAACGCCCCAAGAAAAGAGACTCTAATCCAAAAGAATAACGCTTAACTGATAATGACAAAAATCCTTAGAAACAGACCCCACAGGGAAGTAATACTCCTTGAATCTCTCCCCTTTAAATCTCTCCCCTTCAAAGATTCTATTGTTTCCCAAATTCCTCATAGCAAAGAAACAGACATACCACAAAAACCTATTTCTTATCCTGCTCCGTTCAACAAACTTGTCTTTAATTTAACATATTTTCCCAGTTGTACTGTTTGCCAAAAACTCCCTTTACAAAACCAAGATTAAATTAACCTTACAACTGATGGTCATAAATTTTGCCCATTGCAGGGTGGTTCAGGCTCGGTACAGGGAATACTGTTTTCCAACATTCAAGTTTCGGACGTTCAAACTCCAATCATGATAGACCAATACTACTGCGACAGAGGCAGATGCAATAACGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTGAAACGCCAGTCCGTTTGGCCTGCAGCGACAGCCTTCCCTGCACCGGTGTGTCATTGGACACCATTCAGCTCGAGGGGAGTGGCCGCTCGCCTGAACCGTTCTGTTGGAAGGCATATGGCGAGCTGAAAACTTCCACTGTCCCTCCCATTGATTGCTTGCAGTCTGGCAATCCATCGATCGCTCGGGCTCGCGATTCTTGC

mRNA sequence

ATGGAGATGGGTGGGAAGAATTTGAAATGTCTTACATCTGTTGCTTTCATTTGTGTTTTCGTTTGGTGTTTTAGTTTGCAAAATTGCCATGCAAGAGAAGGCAGGCATTTGACACACACAAAAACTGGGTCAGGTAGAGAGATTGAGAAGAAAGTTAGAGATGACACTGGTTTTTCTGGCCGCCACCGCCACGTTCATCGCCACCGTGGAGGTAGCGGCCCGTCGAGGTCCACAAGCGGTGGCCGTGTCGGAAGTGAGAATATTGTGGGAGTCTCACTAGCAAGTAAAGGTGGTTCTTCCAACTTTAATGTGTTGGACTTTGGAGCTAAGGGTGATGGAAGGGCAGATGACACCAAGGCATTTCAGGCAGCTTGGGAAGCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCGCTGGCTCCGAATTCCTCGTTGGCCCCATCTCGTTCTCGGGTCCTAATTGCCAACCGAACATTGTATTTCAGTTGGATGGCAAAATCATTGCTCCAACAAGCTCAAGTGCTTGGGGTTCAGGTCTCTTACAGTGGATTGAGTTCACTAAACTTAAAGGGATTACAGTGAAGGGTTCAGGAACTATTGATGGACAAGGCTCGGCTTGGTGGAACGACTCACCGACATACAATCCTACGGATGAAAATTTGGACGAGCAAAGCAGTAGCGGAAAACTTCCAAGCACCAAACCAACAGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATTCAAAATAGCCAACAGACTCACCTGAAATTTGATGGTTGCACATCGGTTCAGGTCTTTTCCCTAACCGTTTCATCGCCCGGTGACAGCCCGAACACAGATGGAATTCACTTGCAGAACTCTCAGAACGTGATCATCTCAAACAGCAATCTAGCTTGTGGAGACGATTGTATATCCATACAAACTGGAAGCTCAGGCATATACATACACAACATCAACTGTGGACCAGGCCACGGAATTAGCATTGGAGGGCTAGGACGCGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTTCGCGACATCAAACTACGGAACACAATGACTGGTGTCAGAATAAAAACATGGCAGGGTGGTTCAGGCTCGGTACAGGGAATACTGTTTTCCAACATTCAAGTTTCGGACGTTCAAACTCCAATCATGATAGACCAATACTACTGCGACAGAGGCAGATGCAATAACGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTGAAACGCCAGTCCGTTTGGCCTGCAGCGACAGCCTTCCCTGCACCGGTGTGTCATTGGACACCATTCAGCTCGAGGGGAGTGGCCGCTCGCCTGAACCGTTCTGTTGGAAGGCATATGGCGAGCTGAAAACTTCCACTGTCCCTCCCATTGATTGCTTGCAGTCTGGCAATCCATCGATCGCTCGGGCTCGCGATTCTTGC

Coding sequence (CDS)

ATGGAGATGGGTGGGAAGAATTTGAAATGTCTTACATCTGTTGCTTTCATTTGTGTTTTCGTTTGGTGTTTTAGTTTGCAAAATTGCCATGCAAGAGAAGGCAGGCATTTGACACACACAAAAACTGGGTCAGGTAGAGAGATTGAGAAGAAAGTTAGAGATGACACTGGTTTTTCTGGCCGCCACCGCCACGTTCATCGCCACCGTGGAGGTAGCGGCCCGTCGAGGTCCACAAGCGGTGGCCGTGTCGGAAGTGAGAATATTGTGGGAGTCTCACTAGCAAGTAAAGGTGGTTCTTCCAACTTTAATGTGTTGGACTTTGGAGCTAAGGGTGATGGAAGGGCAGATGACACCAAGGCATTTCAGGCAGCTTGGGAAGCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCGCTGGCTCCGAATTCCTCGTTGGCCCCATCTCGTTCTCGGGTCCTAATTGCCAACCGAACATTGTATTTCAGTTGGATGGCAAAATCATTGCTCCAACAAGCTCAAGTGCTTGGGGTTCAGGTCTCTTACAGTGGATTGAGTTCACTAAACTTAAAGGGATTACAGTGAAGGGTTCAGGAACTATTGATGGACAAGGCTCGGCTTGGTGGAACGACTCACCGACATACAATCCTACGGATGAAAATTTGGACGAGCAAAGCAGTAGCGGAAAACTTCCAAGCACCAAACCAACAGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATTCAAAATAGCCAACAGACTCACCTGAAATTTGATGGTTGCACATCGGTTCAGGTCTTTTCCCTAACCGTTTCATCGCCCGGTGACAGCCCGAACACAGATGGAATTCACTTGCAGAACTCTCAGAACGTGATCATCTCAAACAGCAATCTAGCTTGTGGAGACGATTGTATATCCATACAAACTGGAAGCTCAGGCATATACATACACAACATCAACTGTGGACCAGGCCACGGAATTAGCATTGGAGGGCTAGGACGCGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTTCGCGACATCAAACTACGGAACACAATGACTGGTGTCAGAATAAAAACATGGCAGGGTGGTTCAGGCTCGGTACAGGGAATACTGTTTTCCAACATTCAAGTTTCGGACGTTCAAACTCCAATCATGATAGACCAATACTACTGCGACAGAGGCAGATGCAATAACGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTGAAACGCCAGTCCGTTTGGCCTGCAGCGACAGCCTTCCCTGCACCGGTGTGTCATTGGACACCATTCAGCTCGAGGGGAGTGGCCGCTCGCCTGAACCGTTCTGTTGGAAGGCATATGGCGAGCTGAAAACTTCCACTGTCCCTCCCATTGATTGCTTGCAGTCTGGCAATCCATCGATCGCTCGGGCTCGCGATTCTTGC

Protein sequence

MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSGRHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCLQSGNPSIARARDSC

Homology

[Close]

BLAST of MS016514 vs. NCBI nr
Match: XP_022152131.1 (polygalacturonase At1g48100 [Momordica charantia])

HSP 1 Score: 999.2 bits (2582), Expect = 1.3e-287
Identity = 489/494 (98.99%), Postives = 490/494 (99.19%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG
Sbjct: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAKGDGRADDTKA 120
            HRHVHRH GGSGPSRSTSGGRVGSENIVGVS ASKGGSSNFNVLDFGAKGDGRADDTKA
Sbjct: 61  XHRHVHRHHGGSGPSRSTSGGRVGSENIVGVSPASKGGSSNFNVLDFGAKGDGRADDTKA 120

Query: 121 FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG 180
           FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG
Sbjct: 121 FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG 180

Query: 181 LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTKPTALR 240
           LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSP YNPT+ENLDEQSSSGKLPSTKPTALR
Sbjct: 181 LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPIYNPTNENLDEQSSSGKLPSTKPTALR 240

Query: 241 FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS 300
           FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS
Sbjct: 241 FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS 300

Query: 301 NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT 360
           NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT
Sbjct: 301 NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT 360

Query: 361 MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV 420
           MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV
Sbjct: 361 MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV 420

Query: 421 NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL 480
           NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL
Sbjct: 421 NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL 480

Query: 481 QSGNPSIARARDSC 495
           QSGNPSIARARDSC
Sbjct: 481 QSGNPSIARARDSC 494

[Close]

BLAST of MS016514 vs. NCBI nr
Match: XP_023527919.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.4 bits (2193), Expect = 1.6e-242
Identity = 418/499 (83.77%), Postives = 442/499 (88.58%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL ++AFIC FVW FSLQNC AR G+H  H KTG             G+  
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHWAHRKTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSL-----ASKGGSSNFNVLDFGAKGDGRA 120
           RH H+HRHRGGS  S     G VGSE++VG SL     ASKGGS NFNVLDFGAKGDGRA
Sbjct: 61  RHGHIHRHRGGSSSSGLMDSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQAAWE+ACNAE STVEVP+GSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQAAWESACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
           AWGSGLLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSSGKLPSTK
Sbjct: 181 AWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGVTV+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVTVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIISNSNLACGDDCISIQTGSS +YIHN+NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISNSNLACGDDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTM GVRIKTWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCD G C+NGSSAVAIS
Sbjct: 361 KLQNTMNGVRIKTWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GVNYVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGSGRS EPFCWKAYGELKTSTVP
Sbjct: 421 GVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
            IDCLQSGNP  A+A DSC
Sbjct: 481 AIDCLQSGNPGKAQAHDSC 484

[Close]

BLAST of MS016514 vs. NCBI nr
Match: XP_022935210.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 849.0 bits (2192), Expect = 2.1e-242
Identity = 418/499 (83.77%), Postives = 443/499 (88.78%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL ++AFIC FVW FSLQNC AREG+H  H KTG             G+  
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHWAHRKTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSL-----ASKGGSSNFNVLDFGAKGDGRA 120
           RH  +HR RGGSG SR T  G VGSE++VG SL     ASKGGS NFNVLDFGAKGDGRA
Sbjct: 61  RHGRIHRDRGGSGSSRLTGSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQAAWE+AC+AE STVEVP+GSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQAAWESACSAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
           AWGSGLLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSSGKLPSTK
Sbjct: 181 AWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGVTV+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVTVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIISNSNLACGDDCISIQTGSS +YIHN+NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISNSNLACGDDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTM GVRIKTWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCD G C+NGSSAVAIS
Sbjct: 361 KLQNTMNGVRIKTWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GVNYVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGSG S EPFCWKAYGELKTSTVP
Sbjct: 421 GVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGHSNEPFCWKAYGELKTSTVP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
            IDCLQSGNP  A+A DSC
Sbjct: 481 AIDCLQSGNPGKAQAHDSC 484

[Close]

BLAST of MS016514 vs. NCBI nr
Match: XP_038905486.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 847.4 bits (2188), Expect = 6.1e-242
Identity = 412/499 (82.57%), Postives = 446/499 (89.38%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL  +AFICVFVW FSLQN HAREG+H  H +TG             G+  
Sbjct: 1   MEMGGKNLKCLAIIAFICVFVWSFSLQNSHAREGKHWAHRQTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSLASKG-----GSSNFNVLDFGAKGDGRA 120
           RHRHVHRHRG SG SR    G VGSE++VG SLAS+G     GS NFNVLDFGAKGDG+A
Sbjct: 61  RHRHVHRHRGSSGSSRLKGNGHVGSEDVVGTSLASEGSDSKSGSGNFNVLDFGAKGDGKA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQ+AW++ACNAEGSTVEVP+GSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQSAWQSACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
            WGS LLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSSGKLPSTK
Sbjct: 181 VWGSSLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGV V+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVIVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIIS+SNLACGDDCISIQTGSS +YIH++NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISHSNLACGDDCISIQTGSSSVYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTMTGVRIKTWQGGSGSVQGILFSN+QVSDV+TPI+IDQYYCD GRC+NGSSAVAIS
Sbjct: 361 KLQNTMTGVRIKTWQGGSGSVQGILFSNVQVSDVRTPIIIDQYYCDGGRCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GV+YVNI+GTYT TPV  ACSDSLPCTGV+LDTIQLEGSGRS EPFCWKAYGELKTST+P
Sbjct: 421 GVSYVNIKGTYTSTPVHFACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTIP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
           P+DCLQSGNP  A+A DSC
Sbjct: 481 PVDCLQSGNPMKAQAHDSC 484

[Close]

BLAST of MS016514 vs. NCBI nr
Match: XP_022983321.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 838.6 bits (2165), Expect = 2.8e-239
Identity = 413/499 (82.77%), Postives = 440/499 (88.18%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL ++AFIC FVW FSLQNC AR G+H  H KTG             G+  
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHWAHRKTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSL-----ASKGGSSNFNVLDFGAKGDGRA 120
           RH  +HRHRGGS     T  G VGSE++VG SL     ASKGGS NFNVLDFGAKGDGRA
Sbjct: 61  RHGRIHRHRGGSSSLGLTDSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQAAWE+ACNAE STVEVP+GSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQAAWESACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
           AWGSGLLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSS KLPSTK
Sbjct: 181 AWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSRKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGVTV+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVTVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIISNSNLACGDDCISIQTGSS +YIHN+NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISNSNLACGDDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTM GVRIKTWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCD G C+NGSSAVAIS
Sbjct: 361 KLQNTMNGVRIKTWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GVNYVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGSGRS EPFCWKAYGELKTSTVP
Sbjct: 421 GVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
            I+CLQSGNP  A++ +SC
Sbjct: 481 AIECLQSGNPGKAQSHNSC 484

[Close]

BLAST of MS016514 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-101
Identity = 190/398 (47.74%), Postives = 249/398 (62.56%), Query Frame = 0


Query: 102 FNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIV 161
           F+V  FGA GDG  DDT AFQ AW+AAC  E   V  P G  F +    FSGP C+P +V
Sbjct: 80  FDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLV 139

Query: 162 FQLDGKIIAPTSSSAW--GSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTD 221
           FQLDG ++ P     W       QW+ F +L G T  G GT++G G  WW D P      
Sbjct: 140 FQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWW-DLPC--KPH 199

Query: 222 ENLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSS 281
              D  SSSG  P   PT +RF+ S+ + V G+ IQNS Q H+KFDGC  V +  + +SS
Sbjct: 200 RGPDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISS 259

Query: 282 PGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLG 341
           P  SPNTDGIHL N+++V I NS ++ GDDCISI TG S + I  + CGP HGISIG LG
Sbjct: 260 PKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLG 319

Query: 342 RDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYY 401
              ++ACVSN+TVR+  +R++  G+R+KTWQGG+GSV  +LF NIQ+ +V   I++DQYY
Sbjct: 320 VHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYY 379

Query: 402 CDRGRCNNGSSAVAISGVNYVNIRGTY--TETPVRLACSDSLPCTGVSLDTIQL--EGSG 461
           C    C N +SAV +  V Y NI+GTY     P+  ACSD++ CT +++  ++L  E   
Sbjct: 380 CQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGE 439

Query: 462 RSPEPFCWKAYGELKTSTVPPIDCLQSGNPSIARARDS 494
              +PFCW AYG+ +T T+PPIDCL  G+P +  A DS
Sbjct: 440 LVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDS 471

[Close]

BLAST of MS016514 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-80
Identity = 164/360 (45.56%), Postives = 217/360 (60.28%), Query Frame = 0


Query: 99  SSNFNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQP 158
           S   NV DFGAKGDGR DDTKAF+ AW+AAC++  S V +     +LV PISFSGP C+ 
Sbjct: 87  SKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-CKS 146

Query: 159 NIVFQLDGKIIAPTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPT 218
            +  Q+ G I A    S +      W+ F  ++ + V+G GTI+G G  WW +S   N  
Sbjct: 147 GLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKA 206

Query: 219 DENLDEQSSSGKLP-STKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTV 278
                       LP    PTAL FY S  V V  + I+N+QQ H+ FD C +VQ  +L V
Sbjct: 207 ------------LPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMV 266

Query: 279 SSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGG 338
           ++P +SPNTDGIH+  +QN+ IS+  +  GDDCISI  GS  + +++I CGPGHGISIG 
Sbjct: 267 TAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGS 326

Query: 339 LGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQ 398
           LG   ++A VS+V V   KL  T  GVRIKTWQGGSGS   I F N+++ +V+ PI+IDQ
Sbjct: 327 LGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQ 386

Query: 399 YYCDRGR-CNNGSSAVAISGVNYVNIRGT-YTETPVRLACSDSLPCTGVSLDTIQLEGSG 456
            YCD+ + C   SSAV +  V Y NI+GT  +   +   CS   PC G+ L+ + LE  G
Sbjct: 387 NYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEG 432

[Close]

BLAST of MS016514 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-79
Identity = 160/380 (42.11%), Postives = 226/380 (59.47%), Query Frame = 0


Query: 103 NVLDFGAKGDGRADDTKAFQAAWEAACNAEGST-VEVPAGSEFLVGPISFSGPNCQPNIV 162
           +V +FGAKG+G  DDTKAF  AW+ AC+++  T + VP     L+ PI  SGP C+  + 
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGP-CKARLT 113

Query: 163 FQLDGKIIAPTSSSAW-GSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDE 222
            Q+ G IIAP     W G    +W+ F  L  +TV+G GT++G G  WW  S  +N ++ 
Sbjct: 114 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNP 173

Query: 223 NLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSP 282
                          PTAL F+    + V  + + +SQQ H+    C  V +  L V +P
Sbjct: 174 -----------CRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAP 233

Query: 283 GDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGR 342
             SPNTDGIH+  S+ ++I N+ ++ GDDCISI   S+ I I NI CGPGHGISIG LG+
Sbjct: 234 ATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGK 293

Query: 343 DQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYC 402
            ++   V ++TV    + +T  GVRIKTWQGGSG V  I+F NI++++V  PI+IDQYYC
Sbjct: 294 SKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYC 353

Query: 403 D-RGRCNNGSSAVAISGVNYVNIRGT-YTETPVRLACSDSLPCTGVSLDTIQLEGSGRS- 462
           D R  C N +SA++I  +++V++RGT  ++  ++++CSDS PC  + L  I LE S    
Sbjct: 354 DSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG 413

Query: 463 -PEPFCWKAYGELKTSTVPP 477
             E FCW+AYG       PP
Sbjct: 414 FTESFCWEAYGSSSGQVYPP 421

[Close]

BLAST of MS016514 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 294.3 bits (752), Expect = 2.6e-78
Identity = 157/365 (43.01%), Postives = 224/365 (61.37%), Query Frame = 0


Query: 94  ASKGGSSNFNVLDFGAKGDGRADDTKAFQAAWEAACNAEGS-TVEVPAGSEFLVGPISFS 153
           AS    +  +V DFGAKGDG+ DDT+AF  AW+ AC++ G+  + VP G+ +L+  I  +
Sbjct: 60  ASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLT 119

Query: 154 GPNCQPNIVFQLDGKIIAPTSSSAWGSGLLQWIEFTKLKGITVKG--SGTIDGQGSAWWN 213
           GP C   +  Q+ G + A    S +   + +WI F  +  ++V G  +G +DG G  WW 
Sbjct: 120 GP-CNSILTVQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQ 179

Query: 214 DSPTYNPTDENLDEQSSSGKLPSTK-PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTS 273
           +S   N               P TK PTAL FY S  + V  + ++N+QQ  +  + C++
Sbjct: 180 NSCKRNKAK------------PCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSN 239

Query: 274 VQVFSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGP 333
           VQV ++ V++P DSPNTDGIH+ N+QN+ +S S +  GDDCISI++GS  + I++I CGP
Sbjct: 240 VQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGP 299

Query: 334 GHGISIGGLGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDV 393
           GHGISIG LG D +KA VS VTV   KL  T  GVRIKT+QGGSG+   I+F NIQ+ +V
Sbjct: 300 GHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNV 359

Query: 394 QTPIMIDQYYCDRGRCNNGSSAVAISGVNYVNIRGT-YTETPVRLACSDSLPCTGVSLDT 453
           + PI+IDQ YCD+ +C    SAV +  V Y +I GT  +E  +   CS + PC G+ LD 
Sbjct: 360 KNPIIIDQDYCDKSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDR 410

[Close]

BLAST of MS016514 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.8e-78
Identity = 148/353 (41.93%), Postives = 217/353 (61.47%), Query Frame = 0


Query: 102 FNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIV 161
           FNV  +GA GDG+ D T+AF  AW+AAC    + + VP   +F+V  + F+GP CQP+  
Sbjct: 59  FNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPHFT 118

Query: 162 FQLDGKIIAPTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDEN 221
           F++DG I A  + ++W +  + W++F KL G T+ G G IDGQG  WW     +    E 
Sbjct: 119 FKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGREI 178

Query: 222 LDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPG 281
            +++         +PTA++F  S G+ + G+ + NS + HL F  C  V++  +++++P 
Sbjct: 179 CNDRD--------RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPR 238

Query: 282 DSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRD 341
           DSPNTDGI +  S+N  +  + +  GDDC++I TGSS I I ++ CGPGHGISIG LGR+
Sbjct: 239 DSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRE 298

Query: 342 QTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYC- 401
            ++A VS V V   K  +T  G+RIKTWQGGSG    I++ N+++ + + PI+I+Q+YC 
Sbjct: 299 NSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCT 358

Query: 402 DRGRCNNGSSAVAISGVNYVNIRGT-YTETPVRLACSDSLPCTGVSLDTIQLE 453
               C N  SAV I  V Y NIRGT  T   ++L CSDS+PC  + L  I L+
Sbjct: 359 SASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLK 401

[Close]

BLAST of MS016514 vs. ExPASy TrEMBL
Match: A0A6J1DGP8 (polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111019919 PE=3 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 6.1e-288
Identity = 489/494 (98.99%), Postives = 490/494 (99.19%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG
Sbjct: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAKGDGRADDTKA 120
            HRHVHRH GGSGPSRSTSGGRVGSENIVGVS ASKGGSSNFNVLDFGAKGDGRADDTKA
Sbjct: 61  XHRHVHRHHGGSGPSRSTSGGRVGSENIVGVSPASKGGSSNFNVLDFGAKGDGRADDTKA 120

Query: 121 FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG 180
           FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG
Sbjct: 121 FQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGSG 180

Query: 181 LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTKPTALR 240
           LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSP YNPT+ENLDEQSSSGKLPSTKPTALR
Sbjct: 181 LLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPIYNPTNENLDEQSSSGKLPSTKPTALR 240

Query: 241 FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS 300
           FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS
Sbjct: 241 FYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVIIS 300

Query: 301 NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT 360
           NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT
Sbjct: 301 NSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLRNT 360

Query: 361 MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV 420
           MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV
Sbjct: 361 MTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVNYV 420

Query: 421 NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL 480
           NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL
Sbjct: 421 NIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPIDCL 480

Query: 481 QSGNPSIARARDSC 495
           QSGNPSIARARDSC
Sbjct: 481 QSGNPSIARARDSC 494

[Close]

BLAST of MS016514 vs. ExPASy TrEMBL
Match: A0A6J1F9Z9 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.0e-242
Identity = 418/499 (83.77%), Postives = 443/499 (88.78%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL ++AFIC FVW FSLQNC AREG+H  H KTG             G+  
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHWAHRKTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSL-----ASKGGSSNFNVLDFGAKGDGRA 120
           RH  +HR RGGSG SR T  G VGSE++VG SL     ASKGGS NFNVLDFGAKGDGRA
Sbjct: 61  RHGRIHRDRGGSGSSRLTGSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQAAWE+AC+AE STVEVP+GSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQAAWESACSAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
           AWGSGLLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSSGKLPSTK
Sbjct: 181 AWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGVTV+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVTVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIISNSNLACGDDCISIQTGSS +YIHN+NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISNSNLACGDDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTM GVRIKTWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCD G C+NGSSAVAIS
Sbjct: 361 KLQNTMNGVRIKTWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GVNYVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGSG S EPFCWKAYGELKTSTVP
Sbjct: 421 GVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGHSNEPFCWKAYGELKTSTVP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
            IDCLQSGNP  A+A DSC
Sbjct: 481 AIDCLQSGNPGKAQAHDSC 484

[Close]

BLAST of MS016514 vs. ExPASy TrEMBL
Match: A0A6J1J1T6 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.4e-239
Identity = 413/499 (82.77%), Postives = 440/499 (88.18%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMGGKNLKCL ++AFIC FVW FSLQNC AR G+H  H KTG             G+  
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHWAHRKTG-------------GY-- 60

Query: 61  RHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSL-----ASKGGSSNFNVLDFGAKGDGRA 120
           RH  +HRHRGGS     T  G VGSE++VG SL     ASKGGS NFNVLDFGAKGDGRA
Sbjct: 61  RHGRIHRHRGGSSSLGLTDSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRA 120

Query: 121 DDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSS 180
           DDTKAFQAAWE+ACNAE STVEVP+GSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTS S
Sbjct: 121 DDTKAFQAAWESACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPS 180

Query: 181 AWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPSTK 240
           AWGSGLLQWIEFTKLKGITVKG+GTIDGQGS WWNDSPTY+PTDENLD++SSS KLPSTK
Sbjct: 181 AWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSRKLPSTK 240

Query: 241 PTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQ 300
           PTALRFYGSDGVTV+GITIQNSQQ HLKFD CTSVQV SLT+SSPGDSPNTDGIHLQNSQ
Sbjct: 241 PTALRFYGSDGVTVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQ 300

Query: 301 NVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDI 360
           NVIISNSNLACGDDCISIQTGSS +YIHN+NCGPGHGISIGGLGRDQTKACVSNVTVRD+
Sbjct: 301 NVIISNSNLACGDDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDV 360

Query: 361 KLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAIS 420
           KL+NTM GVRIKTWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCD G C+NGSSAVAIS
Sbjct: 361 KLQNTMNGVRIKTWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAIS 420

Query: 421 GVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVP 480
           GVNYVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGSGRS EPFCWKAYGELKTSTVP
Sbjct: 421 GVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVP 480

Query: 481 PIDCLQSGNPSIARARDSC 495
            I+CLQSGNP  A++ +SC
Sbjct: 481 AIECLQSGNPGKAQSHNSC 484

[Close]

BLAST of MS016514 vs. ExPASy TrEMBL
Match: A0A1S3B8B8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.3e-231
Identity = 401/500 (80.20%), Postives = 431/500 (86.20%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSGREIEKKVRDDTGFSG 60
           MEMG KNLKCLT++AFICVFVW FSL NCHAR+                       G+  
Sbjct: 1   MEMGRKNLKCLTTIAFICVFVWSFSLHNCHARK---------------------TVGY-- 60

Query: 61  RHRHVHRHRGGSGPS-RSTSGGRVGSENIVGVSLASK-----GGSSNFNVLDFGAKGDGR 120
           RH  VHRH  GSG S R T  G VGSE++VG SLASK     GGS NFNVLDFGAKGDG 
Sbjct: 61  RHWQVHRHHQGSGRSLRLTGSGHVGSEDVVGTSLASKGRATNGGSGNFNVLDFGAKGDGE 120

Query: 121 ADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSS 180
            DDTKAFQ+AWE+ACN EGS VEVP+GS FLVGPISFSGPNCQPNI+FQLDGKIIAPTS 
Sbjct: 121 TDDTKAFQSAWESACNVEGSVVEVPSGSNFLVGPISFSGPNCQPNIMFQLDGKIIAPTSP 180

Query: 181 SAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLPST 240
           SAWGSG+LQWIEFTKLKGITVKG+GTIDGQGS WWNDSPT NPTDENLD++SS GKLPST
Sbjct: 181 SAWGSGILQWIEFTKLKGITVKGTGTIDGQGSVWWNDSPTNNPTDENLDKESSGGKLPST 240

Query: 241 KPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNS 300
           KPTALRFYGSD VTV+GITIQNSQ+ HLKFD CT+VQV SLT+SSPGDSPNTDGIHLQNS
Sbjct: 241 KPTALRFYGSDEVTVSGITIQNSQKAHLKFDSCTAVQVSSLTISSPGDSPNTDGIHLQNS 300

Query: 301 QNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRD 360
           QNVIISNSNLACGDDCISIQTGSSG+YIHN+NCGPGHGISIGGLGRD+TKACVSN+TVRD
Sbjct: 301 QNVIISNSNLACGDDCISIQTGSSGVYIHNVNCGPGHGISIGGLGRDETKACVSNITVRD 360

Query: 361 IKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAI 420
           +KL+NTM GVRIKTWQGGSGSVQGILFSNIQV+DV+ PIMIDQYYCD GRC+NGSSAVAI
Sbjct: 361 VKLQNTMNGVRIKTWQGGSGSVQGILFSNIQVTDVRIPIMIDQYYCDGGRCHNGSSAVAI 420

Query: 421 SGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTV 480
           SGVNYVNIRGTYT TPVR ACSDSLPCTGV+LDTIQLEGS  S EPFCWKAYGELKTSTV
Sbjct: 421 SGVNYVNIRGTYTSTPVRFACSDSLPCTGVTLDTIQLEGSDGSNEPFCWKAYGELKTSTV 477

Query: 481 PPIDCLQSGNPSIARARDSC 495
           PP+DCLQSGNP  A+A DSC
Sbjct: 481 PPVDCLQSGNPFKAQAHDSC 477

[Close]

BLAST of MS016514 vs. ExPASy TrEMBL
Match: A0A6J1EJN2 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 9.2e-228
Identity = 400/496 (80.65%), Postives = 426/496 (85.89%), Query Frame = 0


Query: 1   MEMGGKNLKCLTSVAFICVFVWCFSLQNCHAREGRHL-THTKTGSGREIEKKVRDDTGFS 60
           M+M GKNLKCL ++AFICVFVW FSL+NCH REGR    H KTG             G+ 
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTG-------------GY- 60

Query: 61  GRHRHVHRHRGGSGPSRSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAKGDGRADDTK 120
            RH HVH                V SE++VG SLAS+GGS +FNVLDFGAKGDG  DDTK
Sbjct: 61  -RHHHVH----------------VDSEDVVGASLASEGGSGSFNVLDFGAKGDGEEDDTK 120

Query: 121 AFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSSSAWGS 180
           AFQAAWE+ACNAEGS VEVP+G EFLVGPISFSGPNCQPNIVFQLDGKIIAPTS SAWGS
Sbjct: 121 AFQAAWESACNAEGSMVEVPSGYEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGS 180

Query: 181 GLLQWIEFTKLKGITVKGSGTIDGQGSAWW-NDSPTYNPTDENLDEQSSSGKLPSTKPTA 240
           GLLQWIEFTKLKGITVKG+GTIDGQGS WW +DSPT +PTDENLD++SSSG+LP TK TA
Sbjct: 181 GLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDDPTDENLDKESSSGRLPRTKLTA 240

Query: 241 LRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQNSQNVI 300
           LRFYGSDGVTVTGITIQNSQQ HLKFD CTSV V SLT+SSPGDSPNTDGIHLQNSQNVI
Sbjct: 241 LRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVPVSSLTISSPGDSPNTDGIHLQNSQNVI 300

Query: 301 ISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTVRDIKLR 360
           ISNSNLACGDDCISIQTGSS IYIH++NCGPGHGISIGGLGRDQTKACVSNVTVRD+KL+
Sbjct: 301 ISNSNLACGDDCISIQTGSSSIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQ 360

Query: 361 NTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAVAISGVN 420
           NTM GVRIKTWQGGSGSVQGILFSNIQVSDV TP+MIDQYYCD GRC+NGSSAVAISGVN
Sbjct: 361 NTMNGVRIKTWQGGSGSVQGILFSNIQVSDVGTPVMIDQYYCDGGRCHNGSSAVAISGVN 420

Query: 421 YVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSGRSPEPFCWKAYGELKTSTVPPID 480
           YVNIRGTYT TPV LACSDSLPCTGV+LDTIQLEGS RS EPFCWKAYGELKTSTVPPID
Sbjct: 421 YVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSSRSNEPFCWKAYGELKTSTVPPID 465

Query: 481 CLQSGNPSIARARDSC 495
           CLQSGNPS A+A   C
Sbjct: 481 CLQSGNPSKAQAHGFC 465

[Close]

BLAST of MS016514 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-157
Identity = 267/409 (65.28%), Postives = 316/409 (77.26%), Query Frame = 0


Query: 92  SLASKGGSSNFNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISF 151
           S+A  G S   NVLD GAKGDG +DDTKAF+ AW+ AC    ST+ VP+GS FLVGP+SF
Sbjct: 41  SIAEGGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSF 100

Query: 152 SGPNCQPNIVFQLDGKIIAPTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWND 211
            G  C+  IVFQL+GKIIAPTS+SAWGSGLLQWIEF  L+GIT+KG G IDG+GS WWND
Sbjct: 101 LGKECKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWND 160

Query: 212 SPTYNPTDENLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQ 271
                             K+P TKPTALRFYGS+GVTV+GITIQNS QTHLKFD C S+Q
Sbjct: 161 --------------MMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQ 220

Query: 272 VFSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGH 331
           V   T SSPGDSPNTDGIHLQNSQ+ +I  S LACGDDCISIQTG S I IH+++CGPGH
Sbjct: 221 VSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGH 280

Query: 332 GISIGGLGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQT 391
           GISIGGLG+D TKACVSN+TVRD+ +  T  GVRIK+WQGGSGSV+ ++FSNIQVS+V  
Sbjct: 281 GISIGGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVAN 340

Query: 392 PIMIDQYYCDRGRCNNGSSAVAISGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQL 451
           PI+IDQYYCD G C+N +SAVA+S +NY+NI+GTYT+ PVR ACSDSLPCTG+SL TI+L
Sbjct: 341 PIIIDQYYCDGGGCHNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIEL 400

Query: 452 E---GSGRSPEPFCWKAYGELKTSTVPPIDCLQSGNPSIARAR---DSC 495
           +   G   S +PFCWKA+GELKT T+PPI CL++     A +R   D+C
Sbjct: 401 KPATGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

[Close]

BLAST of MS016514 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 528.5 bits (1360), Expect = 5.8e-150
Identity = 271/520 (52.12%), Postives = 342/520 (65.77%), Query Frame = 0


Query: 8   LKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSG------REIEKKVRDDTGFSGR 67
           L+ +T +  + + VW  +L+ C AR GRH  H    S          + K  ++      
Sbjct: 9   LRSITMMTLVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMS 68

Query: 68  HRHVHRHRGGSGPSRSTSGGRVGSE--------------NIVGVSLASKGGSSNFNVLDF 127
           H+   + +  + P +S  G  V S+                  + L     S  FNVLDF
Sbjct: 69  HKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVLDF 128

Query: 128 GAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGK 187
           GAKGDG +DDT+AF+AAW +AC  E ST+ +P    FLVGPISFSGP CQ NIVFQL+G 
Sbjct: 129 GAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGM 188

Query: 188 IIAPTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWW-----------NDSPTYN 247
           I+APT + +WG GL+ WIEFTKL GIT++G+G IDG+G+ WW           +D     
Sbjct: 189 IVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIV 248

Query: 248 PTDENLDEQ-------SSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTS 307
           P + ++ E+         + ++PS KPTALRFYGS  VTVTGITIQNS Q HLKFD C  
Sbjct: 249 PLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVK 308

Query: 308 VQVFSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGP 367
           V V  + VSSPGDSPNTDGIHLQN+++V+I  + LACGDDCISIQTG S +Y+HN+NCGP
Sbjct: 309 VLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGP 368

Query: 368 GHGISIGGLGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDV 427
           GHGISIG LG+D TKACVSN+TVRD+ + NTMTGVRIKTWQGG GSV+GILFSNIQ+++V
Sbjct: 369 GHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEV 428

Query: 428 QTPIMIDQYYCDRGRCNNGSSAVAISGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTI 487
           Q PI+IDQ+YCD  +C N +SAV++ GV Y  IRGTYT  PV  ACSDS PC  V L  I
Sbjct: 429 QLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVKPVHFACSDSFPCIDVQLSGI 488

[Close]

BLAST of MS016514 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 525.8 bits (1353), Expect = 3.8e-149
Identity = 272/518 (52.51%), Postives = 336/518 (64.86%), Query Frame = 0


Query: 7   NLKCLTSVAFICVFVWCFSLQNCHAREGRHLTHTKTGSG------REIEKKVRDDTGFSG 66
           +L+ +T +  + V VW  +L+ C AR GRH  H+   S          + K   ++    
Sbjct: 8   SLRSITMMILMAVLVWSVTLETCIARRGRHWRHSHRSSSDLSDSLSNKKPKSHGNSHHHS 67

Query: 67  RHRHVHRHRGGSGPS----------RSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAK 126
            H + H H+    P            + S    G   +    L  + GS  FNV+DFGAK
Sbjct: 68  SHNNNHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKGSQVFNVMDFGAK 127

Query: 127 GDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIA 186
           GDG+ DDTKAF+AAW AAC  E S + VP    +LVGPISFSGP CQ NIVFQLDG IIA
Sbjct: 128 GDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIA 187

Query: 187 PTSSSAWGSGLLQWIEFTKLKGITVKGSGTIDGQGSAWW-----------------NDSP 246
           PT S  WG GL+ WI+FTKLKGI V+G G IDG+GS WW                 N+S 
Sbjct: 188 PTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIVPLNNSA 247

Query: 247 TYN---PTDENLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSV 306
             N   P    LDE     ++PS KPTALRF GS GV VTGITIQNS Q HLKFD C  V
Sbjct: 248 NQNPPMPIRSELDE-----RMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGV 307

Query: 307 QVFSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPG 366
            V  + VSSPGDSPNTDGIHLQN+++V+I ++ LACGDDCISIQTG S +++HN+NCGPG
Sbjct: 308 VVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPG 367

Query: 367 HGISIGGLGRDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQ 426
           HGISIG LG++ TKACVSN+TVRD+ + NTMTGVRIKTWQGG GSV+GI+FSNIQ++ VQ
Sbjct: 368 HGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQVQ 427

Query: 427 TPIMIDQYYCDRGRCNNGSSAVAISGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQ 486
            PI I+Q+YCD  +C N +SAVA+ GV Y  I+GTYT  PV  ACSD+ PC  V L +I+
Sbjct: 428 IPITINQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDVQLSSIE 487

[Close]

BLAST of MS016514 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 5.7e-113
Identity = 212/442 (47.96%), Postives = 286/442 (64.71%), Query Frame = 0


Query: 60  GRHRHV------HRHRGGSGPSRSTSGGRVGSENIVGVSLASKGGSSNFNVLDFGAKGDG 119
           GR  HV      HR    + P+ S +     S N              FN+  +GAKGDG
Sbjct: 30  GRKHHVKKIKPKHRRHSKNTPTGSPAPAPYPSTN-----------EGVFNIFSYGAKGDG 89

Query: 120 RADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTS 179
            +DD+KA   AW+AAC   G  VE+PAG++FLV  ++  GP C+   V Q++G ++AP  
Sbjct: 90  VSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGP-CKEETVVQIEGILVAPEK 149

Query: 180 SSAW-GSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTDENLDEQSSSGKLP 239
             +W  S L QW+ F  +  +T++GSGT++G+G  WWN   TY         Q+ +  +P
Sbjct: 150 IGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWN-LDTY-------QTQTRNKYIP 209

Query: 240 STKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSSPGDSPNTDGIHLQ 299
             KPTALRFY S+ VTV  I+I NS   HLKFD    V+V ++T+SSP +SPNTDGIHLQ
Sbjct: 210 PMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENSPNTDGIHLQ 269

Query: 300 NSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLGRDQTKACVSNVTV 359
           N++NV I +SN+ACGDDC+SIQTGSS ++IH+INCGPGHGISIGGLG+D++ ACVS++ V
Sbjct: 270 NTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKSVACVSDIIV 329

Query: 360 RDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYYCDRGRCNNGSSAV 419
            DI ++NT+ GVRIKTWQGG G V+ + FSNIQV DV+ PI+IDQYYCD+ +C N + AV
Sbjct: 330 EDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQYYCDKSKCKNQTRAV 389

Query: 420 AISGVNYVNIRGTYTETPVRLACSDSLPCTGVSLDTIQLEGSG--------RSPEPFCWK 479
           +ISGV Y NI G++T  PVR+ACS+++PC  V L  I+L  SG        +  +  CW 
Sbjct: 390 SISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIRGLQTHQQQQALCWN 449

Query: 480 AYGELKTSTVPPI--DCLQSGN 485
           +YG+ +   VP     CL+  N
Sbjct: 450 SYGKTQGPLVPSSIGYCLRKSN 451

[Close]

BLAST of MS016514 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 9.1e-103
Identity = 190/398 (47.74%), Postives = 249/398 (62.56%), Query Frame = 0


Query: 102 FNVLDFGAKGDGRADDTKAFQAAWEAACNAEGSTVEVPAGSEFLVGPISFSGPNCQPNIV 161
           F+V  FGA GDG  DDT AFQ AW+AAC  E   V  P G  F +    FSGP C+P +V
Sbjct: 80  FDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLV 139

Query: 162 FQLDGKIIAPTSSSAW--GSGLLQWIEFTKLKGITVKGSGTIDGQGSAWWNDSPTYNPTD 221
           FQLDG ++ P     W       QW+ F +L G T  G GT++G G  WW D P      
Sbjct: 140 FQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWW-DLPC--KPH 199

Query: 222 ENLDEQSSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDGCTSVQVFSLTVSS 281
              D  SSSG  P   PT +RF+ S+ + V G+ IQNS Q H+KFDGC  V +  + +SS
Sbjct: 200 RGPDGSSSSG--PCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISS 259

Query: 282 PGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSGIYIHNINCGPGHGISIGGLG 341
           P  SPNTDGIHL N+++V I NS ++ GDDCISI TG S + I  + CGP HGISIG LG
Sbjct: 260 PKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLG 319

Query: 342 RDQTKACVSNVTVRDIKLRNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVQTPIMIDQYY 401
              ++ACVSN+TVR+  +R++  G+R+KTWQGG+GSV  +LF NIQ+ +V   I++DQYY
Sbjct: 320 VHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYY 379

Query: 402 CDRGRCNNGSSAVAISGVNYVNIRGTY--TETPVRLACSDSLPCTGVSLDTIQL--EGSG 461
           C    C N +SAV +  V Y NI+GTY     P+  ACSD++ CT +++  ++L  E   
Sbjct: 380 CQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGE 439

Query: 462 RSPEPFCWKAYGELKTSTVPPIDCLQSGNPSIARARDS 494
              +PFCW AYG+ +T T+PPIDCL  G+P +  A DS
Sbjct: 440 LVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDS 471

The following BLAST results are available for this feature:

BLAST of MS016514 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152131.1	1.3e-287	98.99	polygalacturonase At1g48100 [Momordica charantia]	[more]
XP_023527919.1	1.6e-242	83.77	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935210.1	2.1e-242	83.77	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_038905486.1	6.1e-242	82.57	polygalacturonase At1g48100 [Benincasa hispida]	[more]
XP_022983321.1	2.8e-239	82.77	polygalacturonase At1g48100-like [Cucurbita maxima]	[more]

BLAST of MS016514 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.3e-101	47.74	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
P35336	2.1e-80	45.56	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
Q94AJ5	1.4e-79	42.11	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q8RY29	2.6e-78	43.01	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
P43212	5.8e-78	41.93	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]

BLAST of MS016514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DGP8	6.1e-288	98.99	polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111019919 PE=3 ...	[more]
A0A6J1F9Z9	1.0e-242	83.77	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 P...	[more]
A0A6J1J1T6	1.4e-239	82.77	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=...	[more]
A0A1S3B8B8	2.3e-231	80.20	polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1	[more]
A0A6J1EJN2	9.2e-228	80.65	polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 S...	[more]

BLAST of MS016514 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14650.1	1.7e-157	65.28	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	5.8e-150	52.12	Pectin lyase-like superfamily protein	[more]
AT1G10640.1	3.8e-149	52.51	Pectin lyase-like superfamily protein	[more]
AT3G26610.1	5.7e-113	47.96	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	9.1e-103	47.74	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 244..293 e-value: 16.0 score: 14.4 coord: 294..315 e-value: 190.0 score: 10.8 coord: 347..368 e-value: 8.1 score: 15.4 coord: 376..397 e-value: 2800.0 score: 4.1 coord: 317..337 e-value: 7000.0 score: 1.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 134..456 e-value: 2.5E-85 score: 286.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 86..484 e-value: 8.5E-125 score: 418.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 208..234
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 10..485
None	No IPR available	PANTHER	PTHR31375:SF171	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 10..485
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 99..484

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016514.1	MS016514.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity