MS016509 (gene) Bitter gourd (TR) v1

Overview


Name	MS016509
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	50S ribosomal protein L12, chloroplastic-like
Location	scaffold657: 307570 .. 308142 (-)
RNA-Seq Expression	MS016509
Synteny	MS016509

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCACTCTCTCTACAGCTTCTCTCCGCTCCCCATCTTGTTCTTCGTCCTCTTATCCATCAAATTTTTCTCAGTCCCCAAGACCCTTCTCTCTCCATTTCCCATTTTCTCCCTCCTCCAACAATCTCTCTCATCGCCTCGTTCATCTCCGTCCAGTCTCCGCCGCGGCCGCGCCGGAGAAAGTTGTAGAAATCGGCGATGTTATCTCCAAATTGACGCTTGAAGAGGCTCGGATTCTGGTTGACTACCTCCAGGAGAAGCTCGGCGTTTCGGCCGCCGCGTTCGCCCCTGCCGCCGTCGCCGTCGCGCCCGGCGCTGGTGCCGGCGGAGACGTTGCAGCCGCCGCCGTCGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGATGTGCCGAGTAATGCGAGGATCGCTGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAGGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGGATTTCGAAGGAAGAGGCGGAGGACGCGAAGAAGCAGCTCGAAGAGGCTGGGGCGAAGATTGCCATTGTT

mRNA sequence

ATGGCTTCCACTCTCTCTACAGCTTCTCTCCGCTCCCCATCTTGTTCTTCGTCCTCTTATCCATCAAATTTTTCTCAGTCCCCAAGACCCTTCTCTCTCCATTTCCCATTTTCTCCCTCCTCCAACAATCTCTCTCATCGCCTCGTTCATCTCCGTCCAGTCTCCGCCGCGGCCGCGCCGGAGAAAGTTGTAGAAATCGGCGATGTTATCTCCAAATTGACGCTTGAAGAGGCTCGGATTCTGGTTGACTACCTCCAGGAGAAGCTCGGCGTTTCGGCCGCCGCGTTCGCCCCTGCCGCCGTCGCCGTCGCGCCCGGCGCTGGTGCCGGCGGAGACGTTGCAGCCGCCGCCGTCGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGATGTGCCGAGTAATGCGAGGATCGCTGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAGGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGGATTTCGAAGGAAGAGGCGGAGGACGCGAAGAAGCAGCTCGAAGAGGCTGGGGCGAAGATTGCCATTGTT

Coding sequence (CDS)

ATGGCTTCCACTCTCTCTACAGCTTCTCTCCGCTCCCCATCTTGTTCTTCGTCCTCTTATCCATCAAATTTTTCTCAGTCCCCAAGACCCTTCTCTCTCCATTTCCCATTTTCTCCCTCCTCCAACAATCTCTCTCATCGCCTCGTTCATCTCCGTCCAGTCTCCGCCGCGGCCGCGCCGGAGAAAGTTGTAGAAATCGGCGATGTTATCTCCAAATTGACGCTTGAAGAGGCTCGGATTCTGGTTGACTACCTCCAGGAGAAGCTCGGCGTTTCGGCCGCCGCGTTCGCCCCTGCCGCCGTCGCCGTCGCGCCCGGCGCTGGTGCCGGCGGAGACGTTGCAGCCGCCGCCGTCGAGGAGAAGACGGAGTTCGATGTTGTTATTGAGGATGTGCCGAGTAATGCGAGGATCGCTGTGATTAAGGCTGTTAGGGCTTTGACGAGCTTGGCGTTGAAGGAGGCGAAGGAGCTGATTGAAGGTTTGCCGAAGAAGTTTAAAGAAGGGATTTCGAAGGAAGAGGCGGAGGACGCGAAGAAGCAGCTCGAAGAGGCTGGGGCGAAGATTGCCATTGTT

Protein sequence

MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAPEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLEEAGAKIAIV

Homology

[Close]

BLAST of MS016509 vs. NCBI nr
Match: XP_022152130.1 (50S ribosomal protein L12, chloroplastic-like [Momordica charantia])

HSP 1 Score: 334.7 bits (857), Expect = 5.1e-88
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP
Sbjct: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE
Sbjct: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of MS016509 vs. NCBI nr
Match: KAG6588957.1 (50S ribosomal protein L12-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.9 bits (725), Expect = 1.0e-72
Identity = 169/191 (88.48%), Postives = 177/191 (92.67%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASL+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTASLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEARILVDYLQ+KLGVSAAAFAPAAV VAPG+ AGG+ AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARILVDYLQDKLGVSAAAFAPAAVVVAPGS-AGGEDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. NCBI nr
Match: KAG7022725.1 (50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.5 bits (724), Expect = 1.4e-72
Identity = 168/191 (87.96%), Postives = 177/191 (92.67%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASL+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTASLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEAR+LVDYLQ+KLGVSAAAFAPAAV VAPG+ AGG+ AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARVLVDYLQDKLGVSAAAFAPAAVVVAPGS-AGGEDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. NCBI nr
Match: XP_022989170.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 283.1 bits (723), Expect = 1.8e-72
Identity = 170/191 (89.01%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASL+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTASLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEARILVDYLQ+KLGVSAAAFAPAAVAVA G  AGGD AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARILVDYLQDKLGVSAAAFAPAAVAVASGP-AGGDDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. NCBI nr
Match: XP_022928154.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 281.6 bits (719), Expect = 5.2e-72
Identity = 168/191 (87.96%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTA L+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTAFLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEARILVDYLQ+KLGVSAAAFAPAAV VAPG+ AGG+ AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARILVDYLQDKLGVSAAAFAPAAVVVAPGS-AGGEDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. ExPASy Swiss-Prot
Match: P24929 (50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.2e-50
Identity = 132/192 (68.75%), Postives = 154/192 (80.21%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRP-FSLHFPFSPSSNNLSH-RLVHLRPVSAAA 60
           MASTLST +LRSPS S++S  S  +  P P  +L FP    +  L H R   LRP++A  
Sbjct: 1   MASTLSTITLRSPSPSTAS--STHASIPFPKKALEFPI--RTPKLHHRRATFLRPLAAVE 60

Query: 61  APEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAV 120
           APEKVV++GD IS LTL +A+ LV+YLQ+KLGV+AA+FAPAAVA APGA A    A A V
Sbjct: 61  APEKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVAAAPGAAA---EAPAVV 120

Query: 121 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDVVI++VPSNARIA IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EAEDAK
Sbjct: 121 EEKTEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEAEDAK 180

Query: 181 KQLEEAGAKIAI 191
           KQLEEAGAK++I
Sbjct: 181 KQLEEAGAKVSI 185

[Close]

BLAST of MS016509 vs. ExPASy Swiss-Prot
Match: P36688 (50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.4e-50
Identity = 130/192 (67.71%), Postives = 153/192 (79.69%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRP-FSLHFPF-SPSSNNLSHRLVHLRPVSAAA 60
           MASTLST +LRSPS S+++  S  +  P P  +L FP  +P   N   R   LRP++A  
Sbjct: 1   MASTLSTITLRSPSPSTAT--STHASIPFPKKTLEFPIRTPKLQN--RRATFLRPLAAVE 60

Query: 61  APEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAV 120
           APEKVV++GD IS LTL +A+ LV+YLQ+KLGV+AA+FAPAAV  APGA A    A A V
Sbjct: 61  APEKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVVAAPGAAA---EAPAVV 120

Query: 121 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDVVI++VPSNARIA IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EAEDAK
Sbjct: 121 EEKTEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEAEDAK 180

Query: 181 KQLEEAGAKIAI 191
           KQLEEAGAK++I
Sbjct: 181 KQLEEAGAKVSI 185

[Close]

BLAST of MS016509 vs. ExPASy Swiss-Prot
Match: P02398 (50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 PE=1 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 9.8e-50
Identity = 123/194 (63.40%), Postives = 151/194 (77.84%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNL---SHRLVHLRPVSAA 60
           MA+T + A+L  PS +S    S F + P+P  L FPF  ++N +   S R   LRP++A 
Sbjct: 1   MAATTTMATLNLPSLTSHPNSSTFPKHPQP--LQFPFRTTTNPISLSSTRTTRLRPIAAV 60

Query: 61  AAPEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAA 120
            APEK+ ++G  +S LTLEEAR+LVD+LQ+KLGVSAA+FAPAA   APGA A    AA A
Sbjct: 61  EAPEKIEQLGTQLSGLTLEEARVLVDWLQDKLGVSAASFAPAAAVAAPGAPAD---AAPA 120

Query: 121 VEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDA 180
           VEEKTEFDV I++VPSNARI+VIKAVRALTSL LKEAKELIEGLPKK KEG+SK++AEDA
Sbjct: 121 VEEKTEFDVSIDEVPSNARISVIKAVRALTSLGLKEAKELIEGLPKKLKEGVSKDDAEDA 180

Query: 181 KKQLEEAGAKIAIV 192
           KKQLE+AGAK++IV
Sbjct: 181 KKQLEDAGAKVSIV 189

[Close]

BLAST of MS016509 vs. ExPASy Swiss-Prot
Match: P36212 (50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12C PE=2 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 3.5e-47
Identity = 123/190 (64.74%), Postives = 151/190 (79.47%), Query Frame = 0


Query: 2   ASTLSTA-SLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 61
           ++TLS A ++RS S  +S+   +F    +P ++ FPF  SS++ SHR ++LRP+SA  AP
Sbjct: 3   STTLSIATTIRSSSPLTSASTHHFLS--KPTAIEFPFRLSSSS-SHRAINLRPISAVEAP 62

Query: 62  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 121
           EK+ +IG  IS LTLEEARILVDYLQ+K GVS  + APAA AVA  A  G   AAA VEE
Sbjct: 63  EKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVAAPADGG---AAAVVEE 122

Query: 122 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 181
           +TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK 
Sbjct: 123 QTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKKT 182

Query: 182 LEEAGAKIAI 191
           LEEAGAK++I
Sbjct: 183 LEEAGAKVSI 186

[Close]

BLAST of MS016509 vs. ExPASy Swiss-Prot
Match: P36210 (50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12A PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.1e-46
Identity = 123/195 (63.08%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 2   ASTLSTASLRSPSCSSSSYPSNFSQS---PRPFSLHFPF---SPSSNNLSHRLVHLRPVS 61
           ++TLS A+    +  SSSYP+  S +    R  ++ FP      SS+ L+HR  HLRP++
Sbjct: 3   STTLSIAT----TIRSSSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHLRPIA 62

Query: 62  AAAAPEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAA 121
           A  APEK+ +IG  IS LTLEEARILVDYLQ+K GVS  + APAA AVA  A  G   AA
Sbjct: 63  AVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVAAPADGG---AA 122

Query: 122 AAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAE 181
           A VEE+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE
Sbjct: 123 AVVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAE 182

Query: 182 DAKKQLEEAGAKIAI 191
           +AKK LEEAGAK++I
Sbjct: 183 EAKKTLEEAGAKVSI 190

[Close]

BLAST of MS016509 vs. ExPASy TrEMBL
Match: A0A6J1DD25 (50S ribosomal protein L12, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111019918 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.5e-88
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP
Sbjct: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE
Sbjct: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of MS016509 vs. ExPASy TrEMBL
Match: A0A6J1JLN3 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111486322 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 8.6e-73
Identity = 170/191 (89.01%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTASL+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTASLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEARILVDYLQ+KLGVSAAAFAPAAVAVA G  AGGD AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARILVDYLQDKLGVSAAAFAPAAVAVASGP-AGGDDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. ExPASy TrEMBL
Match: A0A6J1EK20 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111435060 PE=3 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.5e-72
Identity = 168/191 (87.96%), Postives = 176/191 (92.15%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           MASTLSTA L+ PSCSSSS+PS FS SP+  SLHFP S SS+NLS RLVHLRP+SA A P
Sbjct: 1   MASTLSTAFLQFPSCSSSSFPSKFSHSPKSKSLHFPSSLSSSNLSLRLVHLRPLSATAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEARILVDYLQ+KLGVSAAAFAPAAV VAPG+ AGG+ AAAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARILVDYLQDKLGVSAAAFAPAAVVVAPGS-AGGEDAAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. ExPASy TrEMBL
Match: A0A6J1J1D9 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481837 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.6e-71
Identity = 167/191 (87.43%), Postives = 173/191 (90.58%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           M+S LSTASLR PSCSSSSYPSNFS S R  S+ FPFS SS NLS R VH RP+SAAA P
Sbjct: 1   MSSALSTASLRLPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEAR LVDYLQEKLGVSAAAFAPAAVAVAPGA +GGD  AAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVAVAPGAASGGD-DAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIE+VPSNARI+VIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. ExPASy TrEMBL
Match: A0A6J1F403 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441912 PE=3 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.1e-71
Identity = 167/191 (87.43%), Postives = 172/191 (90.05%), Query Frame = 0


Query: 1   MASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 60
           M+S LSTASLR PSCSSSSYPSNFS S R  S+ FPFS SS NLS R VH RP+SAAA P
Sbjct: 1   MSSALSTASLRFPSCSSSSYPSNFSHSLRSTSIQFPFSFSSTNLSLRFVHCRPLSAAAVP 60

Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 120
           EKV EIGDVISKLTLEEAR LVDYLQEKLGVSAAAFAPAAV VAPGA AGGD  AAAVEE
Sbjct: 61  EKVTEIGDVISKLTLEEARTLVDYLQEKLGVSAAAFAPAAVVVAPGAAAGGD-DAAAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIE+VPSNARI+VIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEEVPSNARISVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 190

[Close]

BLAST of MS016509 vs. TAIR 10
Match: AT3G27850.1 (ribosomal protein L12-C )

HSP 1 Score: 189.5 bits (480), Expect = 2.5e-48
Identity = 123/190 (64.74%), Postives = 151/190 (79.47%), Query Frame = 0


Query: 2   ASTLSTA-SLRSPSCSSSSYPSNFSQSPRPFSLHFPFSPSSNNLSHRLVHLRPVSAAAAP 61
           ++TLS A ++RS S  +S+   +F    +P ++ FPF  SS++ SHR ++LRP+SA  AP
Sbjct: 3   STTLSIATTIRSSSPLTSASTHHFLS--KPTAIEFPFRLSSSS-SHRAINLRPISAVEAP 62

Query: 62  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAVEE 121
           EK+ +IG  IS LTLEEARILVDYLQ+K GVS  + APAA AVA  A  G   AAA VEE
Sbjct: 63  EKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVAAPADGG---AAAVVEE 122

Query: 122 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 181
           +TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK 
Sbjct: 123 QTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKKT 182

Query: 182 LEEAGAKIAI 191
           LEEAGAK++I
Sbjct: 183 LEEAGAKVSI 186

[Close]

BLAST of MS016509 vs. TAIR 10
Match: AT3G27830.1 (ribosomal protein L12-A )

HSP 1 Score: 185.7 bits (470), Expect = 3.6e-47
Identity = 123/195 (63.08%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 2   ASTLSTASLRSPSCSSSSYPSNFSQS---PRPFSLHFPF---SPSSNNLSHRLVHLRPVS 61
           ++TLS A+    +  SSSYP+  S +    R  ++ FP      SS+ L+HR  HLRP++
Sbjct: 3   STTLSIAT----TIRSSSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHLRPIA 62

Query: 62  AAAAPEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAA 121
           A  APEK+ +IG  IS LTLEEARILVDYLQ+K GVS  + APAA AVA  A  G   AA
Sbjct: 63  AVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAAAAVAAPADGG---AA 122

Query: 122 AAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAE 181
           A VEE+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE
Sbjct: 123 AVVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAE 182

Query: 182 DAKKQLEEAGAKIAI 191
           +AKK LEEAGAK++I
Sbjct: 183 EAKKTLEEAGAKVSI 190

[Close]

BLAST of MS016509 vs. TAIR 10
Match: AT3G27840.1 (ribosomal protein L12-B )

HSP 1 Score: 171.4 bits (433), Expect = 7.0e-43
Identity = 110/193 (56.99%), Postives = 136/193 (70.47%), Query Frame = 0


Query: 2   ASTLSTASLRSPSCSSSSYPSNFSQSPRPFSLHFPFS---PSSNNLSHRLVHLRPVSAAA 61
           A+TLS A+    S  SS   S      RP S+ FPFS    SS+ LSHR ++L P+SA  
Sbjct: 3   ATTLSIATTIRSSSFSSGLASAHHFPSRPLSIEFPFSFGVSSSSTLSHRAIYLHPISAVK 62

Query: 62  APEKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAGGDVAAAAV 121
            P+K+ +IG  IS LTLEE+RILVDY+Q+K GVS    APAA A+ P    GG  A A+V
Sbjct: 63  TPKKIKKIGSEISSLTLEESRILVDYVQDKFGVSILFSAPAAAALPPPLDNGG--ATASV 122

Query: 122 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 181
           E +T FDVVI DVP   RIAVI A+RA+TSL+L E+KELIEG PKKFKEG++K+EAE+ K
Sbjct: 123 ERQTTFDVVINDVPRGNRIAVITAIRAMTSLSLSESKELIEGFPKKFKEGVTKDEAEEDK 182

Query: 182 KQLEEAGAKIAIV 192
            QLEEAGAK++IV
Sbjct: 183 TQLEEAGAKVSIV 193

[Close]

BLAST of MS016509 vs. TAIR 10
Match: AT4G36420.1 (Ribosomal protein L12 family protein )

HSP 1 Score: 75.9 bits (185), Expect = 4.0e-14
Identity = 58/142 (40.85%), Postives = 83/142 (58.45%), Query Frame = 0


Query: 55  SAAAAPEKVVEIGDVISKLTLEEARILVDYLQEKLGVS----AAAFAPAAVAVAPGA--G 114
           S A   E V +I + +S LTL E   L + L++KL +S     AA  P       GA   
Sbjct: 37  SPATQSENVSKIVNELSNLTLLETMDLTEILRQKLNISELPVMAAMMPGMSLPGSGASKS 96

Query: 115 AGGDVAAAAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEG 174
           AGG+      E KT FDV+++   + ++I VIK VR +T+L LKEAK+L+E  P   K+G
Sbjct: 97  AGGEGKEKKKEAKTAFDVMLQAYDAVSKIKVIKEVRTITALGLKEAKDLVEKAPTLLKKG 156

Query: 175 ISKEEAEDAKKQLEEAGAKIAI 191
           +SKEEAE   ++L+  GAK+A+
Sbjct: 157 VSKEEAEKIIEKLKAVGAKVAM 178

[Close]

BLAST of MS016509 vs. TAIR 10
Match: AT1G70190.1 (Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like )

HSP 1 Score: 65.5 bits (158), Expect = 5.4e-11
Identity = 50/142 (35.21%), Postives = 75/142 (52.82%), Query Frame = 0


Query: 61  EKVVEIGDVISKLTLEEARILVDYLQEKLGVSAAAFAPAAVAVAPGAGAG---------- 120
           ++V  + D IS LTL E   L   + +K G++     P    + PGAG G          
Sbjct: 69  DRVFRLVDEISSLTLLEISELGSIIMKKKGMTE---LPTVAVMKPGAGGGVGVGGGGGMV 128

Query: 121 --GDVAAAAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEG 180
               V+  A  EKT F++ +E   ++A+I +IK +R+ T L LKEAK L+E  P   K G
Sbjct: 129 SQSGVSEEAKVEKTVFEIKLESFEASAKIKIIKELRSFTDLGLKEAKALVEKTPAILKAG 188

Query: 181 ISKEEAEDAKKQLEEAGAKIAI 191
           +SKEE E   ++L+  GAK+ +
Sbjct: 189 LSKEEGEKILEKLKALGAKVVL 207

The following BLAST results are available for this feature:

BLAST of MS016509 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152130.1	5.1e-88	100.00	50S ribosomal protein L12, chloroplastic-like [Momordica charantia]	[more]
KAG6588957.1	1.0e-72	88.48	50S ribosomal protein L12-1, chloroplastic, partial [Cucurbita argyrosperma subs...	[more]
KAG7022725.1	1.4e-72	87.96	50S ribosomal protein L12, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022989170.1	1.8e-72	89.01	50S ribosomal protein L12, chloroplastic-like [Cucurbita maxima]	[more]
XP_022928154.1	5.2e-72	87.96	50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MS016509 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24929	1.2e-50	68.75	50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 P...	[more]
P36688	4.4e-50	67.71	50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV...	[more]
P02398	9.8e-50	63.40	50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 P...	[more]
P36212	3.5e-47	64.74	50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]
P36210	5.1e-46	63.08	50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]

BLAST of MS016509 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DD25	2.5e-88	100.00	50S ribosomal protein L12, chloroplastic-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1JLN3	8.6e-73	89.01	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1EK20	2.5e-72	87.96	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1J1D9	1.6e-71	87.43	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1F403	2.1e-71	87.43	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of MS016509 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27850.1	2.5e-48	64.74	ribosomal protein L12-C	[more]
AT3G27830.1	3.6e-47	63.08	ribosomal protein L12-A	[more]
AT3G27840.1	7.0e-43	56.99	ribosomal protein L12-B	[more]
AT4G36420.1	4.0e-14	40.85	Ribosomal protein L12 family protein	[more]
AT1G70190.1	5.4e-11	35.21	Ribosomal protein L7/L12, oligomerisation;Ribosomal protein L7/L12, C-terminal/a...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 174..191
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR45987:SF16	50S RIBOSOMAL PROTEIN L12-1, CHLOROPLASTIC-RELATED	coord: 1..190
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 120..190 e-value: 9.1E-26 score: 91.8
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	SUPERFAMILY	54736	ClpS-like	coord: 119..190
IPR008932	Ribosomal protein L7/L12, oligomerisation	PFAM	PF16320	Ribosomal_L12_N	coord: 63..110 e-value: 1.3E-12 score: 47.2
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	GENE3D	1.20.5.710	Single helix bin	coord: 62..119 e-value: 5.5E-13 score: 50.7
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	SUPERFAMILY	48300	Ribosomal protein L7/12, oligomerisation (N-terminal) domain	coord: 64..126
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 124..190 e-value: 4.1E-24 score: 84.7
IPR000206	Ribosomal protein L7/L12	TIGRFAM	TIGR00855	TIGR00855	coord: 65..190 e-value: 3.6E-32 score: 109.4
IPR000206	Ribosomal protein L7/L12	PANTHER	PTHR45987	39S RIBOSOMAL PROTEIN L12	coord: 1..190
IPR000206	Ribosomal protein L7/L12	HAMAP	MF_00368	Ribosomal_L7_L12	coord: 58..191 score: 22.568754
IPR000206	Ribosomal protein L7/L12	CDD	cd00387	Ribosomal_L7_L12	coord: 61..190 e-value: 5.15557E-40 score: 130.738

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016509.1	MS016509.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome