MS016276 (gene) Bitter gourd (TR) v1

Overview


Name	MS016276
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	scaffold9_2: 1651133 .. 1653343 (-)
RNA-Seq Expression	MS016276
Synteny	MS016276

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGCATCGAAGGAACCGAAGATAATTGTGAAGTCTCCAATGTCATTTGGGAGGAGAAAATGGACCAAGATAGATAAGACAAATGCTTGCATTCTCCTCTTTTTGCACGTGCTTTGTATTTTTGCACCTTTTCAGTTCAATTGGAGTGCATTTTGGATTGCTTTTGCATTATATATTATTACAGGTCTCTTCGGTATCACTATTTCGTATCACCGAAATCTTTCACATAGAAGTTTCAAGCTCCCTAAATGGCTTGAATACTTATTTGCTTACTGTGGAGTTCAATCACTTCAGGTTGGACATTCAACGTCTTGTACTTTTGCTATTATAATGTCAAATAATTAGTATTAGGATGAATTTACAAATTGATTGGTAAACCCTAAAAAACCTGTCAAGATATCTATTATCTTTTTTAAAAAATTATTGTTTTATTGTTCTATTTATTTTTTTTTTCATGCTAGGGCGATCCAATTTATTGGGTGAGCACGCATAGGTATCATCATCGATTTGTTGATACTGAAAAAGACCCACACAGTCCTATTCAAGGGTTTTGGTTTAGTTATATTTTTTGGATTTTTGATTCCAATACTTTAACAAATAAAATGAGTAATTTTCAAGAGAGACCAGATAGAAGTACATCCGTGATGATTATGAAGCACAATAGACTCGATAATGTTGGTGACTTGAAGAAGCAAACGTTCTATACATTTATCCACAATACATATATTCTACATCCAATTGCTCTTGCAGTCATACTCTATGCTGGTGGAGGAATTCCTTTTCTGATATGGGGAATGGTAAAATAAAATCCTTTTAACTTCTAGTCTTTGAATCATCCACAGAAGATTATATTTAGGTAGGTTACCATAATTTGAATAATAAGACTTTAATAAATTAGTTGTAATTTAATTAAATAAGACTACTCCATCTCATAATGGTATTGTTATTATTATTATTTCTAATTATGTTTGTTTATTTAATAATTTTATTTGTTTATAGGGAAATAGGAAATGAACAAATTCAAATAAAACAATATATATTTTCTTGAAATACGATTTTACATTTTGTTTTTGGAACTAATAATCTAGAAGAGGGTTATTAAATCTATTGAGCAAGAAAAATATGACATATGGTACCTTTTGACAAGCAGTGTGTGAGAAGTGTAATGCTTTTACATGCAACATATTTGGTGAATTCAATATGTCATTTATGGGGAAAACAACCATGGAAAACAGGCGATTTGTCTAGAAATAATTGGTAAGTATGTCTTTCTTTACGTCCAAATAGATGAAAATTGAATTAGTTTATTGTGCTTTTAATGATTTTTTTCTTCCACTGTAACAATATTTTTAGATGGCAAATTTGATGGAATGACTTCTAAGTTATGTAAAAAATGATGGATTTTTTCATTTTTTAGTACAACAAATGGGTTAGGAGATTCGAACTCATGACTTGTTGATGAGAGATAATTGCAATNAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAGAAAGAAGATAGAATTTATTTAGAACTTTAATATATTAAATATTTTCATTATTTAGCATATTTTATATATCAACGAATTTATATACGTAAATTTTAACTTTATATATAAAATATATATTAGTACAACATGTAGAAGTAGGGATTCGAACTCACGATCGTTTGGTTGGAGATAGGTGTCTTAACTAGTTGATCTATGTTTGAGTTGGCTAAATTTTTAGCTAATAAATGTTTAAATTAATGTCAGTTTGATTATTTTGTAATTAAATTAAAGACGCGATTATTTTAATTAAAAGGAACCCATGAATCTTGATTGGAATCATATATTCAAATATATAACATTTTCCTTCTCCTTGGAAGATATACAGCAAATATAATTGAATAACTATTGAATTTTATATTGATTGAGTAATCAATTGTTTATGTAACAATAGTATTATATTTTTACGTCTATTATATATAATTTTTGGATCCTTCATAGGTTGATAAATTTGATTGCATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAATATTCCGCTAGGATGGGCTTTGAATGGTGGCAGTTTGACCCGGGTTGGTATGTTGTTATATTTCTTCAAGCCATTGGATTGGCCACTCAAGTAAAATTACCCTCTCAAAGCCACAAGCAAAAAATGGCT

mRNA sequence

ATGGACGCATCGAAGGAACCGAAGATAATTGTGAAGTCTCCAATGTCATTTGGGAGGAGAAAATGGACCAAGATAGATAAGACAAATGCTTGCATTCTCCTCTTTTTGCACGTGCTTTGTATTTTTGCACCTTTTCAGTTCAATTGGAGTGCATTTTGGATTGCTTTTGCATTATATATTATTACAGGTCTCTTCGGTATCACTATTTCGTATCACCGAAATCTTTCACATAGAAGTTTCAAGCTCCCTAAATGGCTTGAATACTTATTTGCTTACTGTGGAGTTCAATCACTTCAGGGCGATCCAATTTATTGGGTGAGCACGCATAGGTATCATCATCGATTTGTTGATACTGAAAAAGACCCACACAGTCCTATTCAAGGGTTTTGGTTTAGTTATATTTTTTGGATTTTTGATTCCAATACTTTAACAAATAAAATGAGTAATTTTCAAGAGAGACCAGATAGAAGTACATCCGTGATGATTATGAAGCACAATAGACTCGATAATGTTGGTGACTTGAAGAAGCAAACGTTCTATACATTTATCCACAATACATATATTCTACATCCAATTGCTCTTGCAGTCATACTCTATGCTGGTGGAGGAATTCCTTTTCTGATATGGGGAATGTGTGTGAGAAGTGTAATGCTTTTACATGCAACATATTTGGTGAATTCAATATGTCATTTATGGGGAAAACAACCATGGAAAACAGGCGATTTGTCTAGAAATAATTGGTTGATAAATTTGATTGCATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAATATTCCGCTAGGATGGGCTTTGAATGGTGGCAGTTTGACCCGGGTTGGTATGTTGTTATATTTCTTCAAGCCATTGGATTGGCCACTCAAGTAAAATTACCCTCTCAAAGCCACAAGCAAAAAATGGCT

Coding sequence (CDS)

ATGGACGCATCGAAGGAACCGAAGATAATTGTGAAGTCTCCAATGTCATTTGGGAGGAGAAAATGGACCAAGATAGATAAGACAAATGCTTGCATTCTCCTCTTTTTGCACGTGCTTTGTATTTTTGCACCTTTTCAGTTCAATTGGAGTGCATTTTGGATTGCTTTTGCATTATATATTATTACAGGTCTCTTCGGTATCACTATTTCGTATCACCGAAATCTTTCACATAGAAGTTTCAAGCTCCCTAAATGGCTTGAATACTTATTTGCTTACTGTGGAGTTCAATCACTTCAGGGCGATCCAATTTATTGGGTGAGCACGCATAGGTATCATCATCGATTTGTTGATACTGAAAAAGACCCACACAGTCCTATTCAAGGGTTTTGGTTTAGTTATATTTTTTGGATTTTTGATTCCAATACTTTAACAAATAAAATGAGTAATTTTCAAGAGAGACCAGATAGAAGTACATCCGTGATGATTATGAAGCACAATAGACTCGATAATGTTGGTGACTTGAAGAAGCAAACGTTCTATACATTTATCCACAATACATATATTCTACATCCAATTGCTCTTGCAGTCATACTCTATGCTGGTGGAGGAATTCCTTTTCTGATATGGGGAATGTGTGTGAGAAGTGTAATGCTTTTACATGCAACATATTTGGTGAATTCAATATGTCATTTATGGGGAAAACAACCATGGAAAACAGGCGATTTGTCTAGAAATAATTGGTTGATAAATTTGATTGCATTTGGAGAAGGTTGGCACAATAACCACCATGCTTTTGAATATTCCGCTAGGATGGGCTTTGAATGGTGGCAGTTTGACCCGGGTTGGTATGTTGTTATATTTCTTCAAGCCATTGGATTGGCCACTCAAGTAAAATTACCCTCTCAAAGCCACAAGCAAAAAATGGCT

Protein sequence

MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA

Homology

[Close]

BLAST of MS016276 vs. NCBI nr
Match: XP_022153399.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 658.3 bits (1697), Expect = 3.3e-185
Identity = 305/309 (98.71%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDASKEPKIIVKSPMSFGRRKWTK+DKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI
Sbjct: 3   MDASKEPKIIVKSPMSFGRRKWTKMDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 62

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHH+FVDTEK
Sbjct: 63  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHQFVDTEK 122

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY
Sbjct: 123 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 182

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
           TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG
Sbjct: 183 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 242

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP
Sbjct: 243 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 302

Query: 301 SQSHKQKMA 310
           SQSHKQK++
Sbjct: 303 SQSHKQKLS 311

[Close]

BLAST of MS016276 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 480.3 bits (1235), Expect = 1.2e-131
Identity = 215/312 (68.91%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MDASK-EPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALY 60
           M+ASK EPK ++K P  + RRKWTK+DK  AC L  +H+LCI APFQFNWSAFW+AF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  IITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTE 120
           I+TGLFG+T+SYHRNL+H+SFKLPKWLEYLFAYCGVQ+LQGDP+ WVSTHR HH+FVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 KDPHSPIQGFWFSYIFWIFDSNTLTNKM-----SNFQERPDRSTSVMIMKHNRLDNVGDL 180
           +DPHSPI+GFWFS+I W+FDS  LT K+     SNFQ + +R+  V+  KH + +NV DL
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQ-KLERNIFVLFSKHGKPENVADL 180

Query: 181 KKQTFYTFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGK 240
           +KQ FY FIH TY+LHPI LAVILY  GGIPFL+WGM VR V++LH T +VNS+CH+WGK
Sbjct: 181 EKQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGK 240

Query: 241 QPWKTGDLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLA 300
           Q W TGD+S+NNW ++LI FGEGWHNNHHAFEYSA+ G EWWQ D GWY+++FLQAIG+A
Sbjct: 241 QSWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVA 300

Query: 301 TQVKLPSQSHKQ 307
           T VKLPSQSH Q
Sbjct: 301 THVKLPSQSHMQ 311

[Close]

BLAST of MS016276 vs. NCBI nr
Match: XP_022956258.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 478.0 bits (1229), Expect = 6.1e-131
Identity = 217/309 (70.23%), Postives = 247/309 (79.94%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDASKEPK IVK PMSFG++KWTKIDK  A    FLH LCIFAPFQ NWSA  I+F LYI
Sbjct: 1   MDASKEPKSIVKPPMSFGKKKWTKIDKKVAFTFFFLHFLCIFAPFQINWSAISISFTLYI 60

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           I GLFGITISYHRNLSH+SFK+PKWLEYLFAYCGV + QGDPI WVSTHRYHH+FVDTEK
Sbjct: 61  IIGLFGITISYHRNLSHKSFKIPKWLEYLFAYCGVHAFQGDPINWVSTHRYHHQFVDTEK 120

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPI+GFW S+I W+               + D S S M+ +H + DN  DL+ QTFY
Sbjct: 121 DPHSPIKGFWSSHIIWLL-------------YKGDGSLSEMLGRHRKWDNACDLENQTFY 180

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
            FIH TY LHPIALA+ILY  GG P+ IWGMCVRSV+ LH T++VNS+CH+WGKQ WKT 
Sbjct: 181 RFIHKTYFLHPIALALILYGIGGFPYFIWGMCVRSVIFLHGTFMVNSVCHIWGKQQWKTN 240

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNW I+L+ FGEGWHNNHHAFEYSAR+G EWWQ+DPGWYV+ FL+AIG+AT VKLP
Sbjct: 241 DLSRNNWWISLLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLP 296

Query: 301 SQSHKQKMA 310
           SQ H QK+A
Sbjct: 301 SQRHMQKLA 296

[Close]

BLAST of MS016276 vs. NCBI nr
Match: XP_022955961.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-130
Identity = 214/309 (69.26%), Postives = 251/309 (81.23%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDAS+EPK IVK PMS G+RKW K+DK  A  L+FLH LCIFAP Q N+ A  I F L+I
Sbjct: 1   MDASREPKTIVKPPMSLGKRKWNKMDKKLAFALVFLHFLCIFAPLQINFGALSICFCLFI 60

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           ITGLFGIT+SYHRNLSH+SFK+PKWLEY+FAYCGV +LQGDPIYWVSTHRYHH+FVDTE+
Sbjct: 61  ITGLFGITLSYHRNLSHKSFKVPKWLEYVFAYCGVHALQGDPIYWVSTHRYHHQFVDTEQ 120

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPI+GFWFS++ W             F  + D S S M+ +H +LDN  DL+ QTFY
Sbjct: 121 DPHSPIKGFWFSHLIW-------------FLHKWDGSASEMVARHEKLDNARDLENQTFY 180

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
            FI  TY LH I LA+ILY  GGIP+ IWG CVR+VM+LH+T++VNSICH+WGKQPWKT 
Sbjct: 181 KFIRKTYFLHSIVLAIILYGIGGIPYFIWGTCVRTVMILHSTFMVNSICHIWGKQPWKTN 240

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNW ++L+AFGEGWHNNHHAFEYSAR+G EWWQFDPGWYV++FLQAIG+AT VKLP
Sbjct: 241 DLSRNNWWMSLLAFGEGWHNNHHAFEYSARLGIEWWQFDPGWYVIVFLQAIGVATHVKLP 296

Query: 301 SQSHKQKMA 310
           SQS  QK+A
Sbjct: 301 SQSQMQKLA 296

[Close]

BLAST of MS016276 vs. NCBI nr
Match: XP_023526904.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.9 bits (1226), Expect = 1.4e-130
Identity = 216/309 (69.90%), Postives = 248/309 (80.26%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDASKEPK IVK PMSFG++KWTKIDK  A    FLH LC+FAPFQ NWSA  I+F LYI
Sbjct: 1   MDASKEPKSIVKPPMSFGKKKWTKIDKKVAFTFFFLHFLCLFAPFQINWSALSISFTLYI 60

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           ITGLFGITISYHRNLSH+SFK+PKWLEYLFAYC V + QGDPI WVSTHRYHH+FVDTEK
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKWLEYLFAYCAVHAFQGDPINWVSTHRYHHQFVDTEK 120

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPI+GFW S+I W+  +              D S S M+  + + DN  DL+ QTFY
Sbjct: 121 DPHSPIKGFWSSHIIWLLYTG-------------DGSLSEMLGTNRKWDNACDLENQTFY 180

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
            FIH TY LHPIALA+ILY  GG P+ IWGMCVRSV+ LH T++VNS+CH+WGKQPWKT 
Sbjct: 181 RFIHKTYFLHPIALALILYGIGGFPYFIWGMCVRSVIFLHGTFMVNSVCHIWGKQPWKTN 240

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNW I+L+ FGEGWHNNHHAFEYSAR+G EWWQ+DPGWYV+ FL+AIG+AT VKLP
Sbjct: 241 DLSRNNWWISLLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLP 296

Query: 301 SQSHKQKMA 310
           SQ+H QK+A
Sbjct: 301 SQTHMQKLA 296

[Close]

BLAST of MS016276 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 402.1 bits (1032), Expect = 5.6e-111
Identity = 180/298 (60.40%), Postives = 224/298 (75.17%), Query Frame = 0


Query: 12  KSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISY 71
           K  + +  R+W  +D     ++L +H+L + APFQFNW A  +AF LYI+TGL GIT+S+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 72  HRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWF 131
           HRNLSH++FKLPKWLEYLFAYCG Q+LQG+PI WVSTHRYHH+F D+++DPHSP+ GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 132 SYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHP 191
           S++ W+FD+NT+T +                      +NVGDL+KQ FY F+  TYILHP
Sbjct: 208 SHMNWMFDTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYILHP 267

Query: 192 IALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINL 251
           +ALAV LYA GG PF++WGM VR V + H T+LVNS CH+WGKQ W TGDLS+NNW +  
Sbjct: 268 LALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAA 327

Query: 252 IAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           +AFGEGWHNNHHAFE+SAR G EWWQ D  WYVV FLQAIGLAT VKLPS++ KQ+MA
Sbjct: 328 LAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

[Close]

BLAST of MS016276 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.3e-101
Identity = 158/290 (54.48%), Postives = 206/290 (71.03%), Query Frame = 0


Query: 20  RKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLSHRS 79
           RKW   D  +   +  LHVLC+FAP  F+W +FW+ FALY I G+FG T+S+HRNL+HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 80  FKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFWIFD 139
           FKLPK+LEY FAY G+ +LQGDP++WVSTHRYHH++ DT  DPHSPI+GFWF +IFW+FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 140 SNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAVILY 199
           S                    +I K  R +N GDL KQ++Y F+  T++ H    A +LY
Sbjct: 158 S------------------KYIIEKCGRYENAGDLMKQSYYRFLERTFVYHVYLQAALLY 217

Query: 200 AGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGEGWH 259
             GG PF++WGM VR+++  H ++LVNS+CH WG +PW TGDLS NNW I ++  GEGWH
Sbjct: 218 LFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSGEGWH 277

Query: 260 NNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           NNHHAFEYSAR G EWWQ D  WY++  L+ +GLAT +K+PS+ HK+KM+
Sbjct: 278 NNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMS 309

[Close]

BLAST of MS016276 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 7.5e-100
Identity = 160/290 (55.17%), Postives = 205/290 (70.69%), Query Frame = 0


Query: 20  RKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLSHRS 79
           R W   D  +  ++  LHVLC+ APF F+WSAFW++  LY + G+FG T+SYHRNL+HRS
Sbjct: 35  RVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRS 94

Query: 80  FKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFWIFD 139
           FKLPK+LEY FAY G+ +LQGDP++WVSTHRYHH+F DT  DPHSPI+GFWFS+IFW+FD
Sbjct: 95  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFD 154

Query: 140 SNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAVILY 199
           S  +  +                    R +N GDL KQ++Y F+  T++ H    A +LY
Sbjct: 155 SKYILEEC------------------GRYENAGDLLKQSYYRFLERTFVFHVYLQAALLY 214

Query: 200 AGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGEGWH 259
             GG PF++WGM VR+V   HA++LVNS+CH +G Q W TGDLS NNW I ++  GEGWH
Sbjct: 215 MFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWH 274

Query: 260 NNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           NNHHAFEYSAR G EWWQ D  WYV+  L+ +GLAT VK+PS+ HK+KM+
Sbjct: 275 NNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHKRKMS 306

[Close]

BLAST of MS016276 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.3e-95
Identity = 159/301 (52.82%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 9   IIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALY-IITGLFGI 68
           ++ +    F RRKW   D  NA  LL LH L   APF F+W AFWI+F L    +G+ GI
Sbjct: 68  VLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGI 127

Query: 69  TISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQ 128
           T+ +HR L+H  FKLPK +EY FAYCG  +LQGDP+ WVS HRYHH+FVDTE+D HSP Q
Sbjct: 128 TLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQ 187

Query: 129 GFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTY 188
           GFWF +I W+ D +    K                 +  R +NV DLKKQ FY F+  TY
Sbjct: 188 GFWFCHIGWVLDKDLFVEK-----------------RGGRRNNVNDLKKQAFYRFLQKTY 247

Query: 189 ILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNW 248
           + H +AL  +LY  GG P+++WGM  R V + H+T+ +NS+CH WG +PW TGDLS NN 
Sbjct: 248 MYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNM 307

Query: 249 LINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQK 308
            + L AFGEGWHNNHHAFE SAR G EWW+ D  WYV+  LQAIGLAT VKLP+++ KQK
Sbjct: 308 FVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQKQK 351

[Close]

BLAST of MS016276 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.4e-93
Identity = 153/290 (52.76%), Postives = 198/290 (68.28%), Query Frame = 0


Query: 20  RKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLSHRS 79
           RKW ++D   A   LF+H LC+ APF F W A  +A  +Y + GL GIT+SYHRNL+HRS
Sbjct: 32  RKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHRNLAHRS 91

Query: 80  FKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFWIFD 139
           FK+PKWLEY FAYCG+ ++QGDPI WVSTHRYHH+F D+++DPHSP +GFWFS++ W+FD
Sbjct: 92  FKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFD 151

Query: 140 SNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAVILY 199
                             T  ++ K  R  NV DLK+Q +Y F+  T + H +    +LY
Sbjct: 152 ------------------TGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLY 211

Query: 200 AGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGEGWH 259
             GG+ FL WGM +   M  H T L+NS+CH+WG + WKT D SRN W +++ +FGE WH
Sbjct: 212 YFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWH 271

Query: 260 NNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           NNHHAFE SAR G EWWQ D  WY+V FL+ IGLAT VKLPS+S +++MA
Sbjct: 272 NNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 302

[Close]

BLAST of MS016276 vs. ExPASy TrEMBL
Match: A0A6J1DIY2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020911 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 1.6e-185
Identity = 305/309 (98.71%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDASKEPKIIVKSPMSFGRRKWTK+DKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI
Sbjct: 3   MDASKEPKIIVKSPMSFGRRKWTKMDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 62

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHH+FVDTEK
Sbjct: 63  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHQFVDTEK 122

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY
Sbjct: 123 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 182

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
           TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG
Sbjct: 183 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 242

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP
Sbjct: 243 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 302

Query: 301 SQSHKQKMA 310
           SQSHKQK++
Sbjct: 303 SQSHKQKLS 311

[Close]

BLAST of MS016276 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.9e-132
Identity = 215/312 (68.91%), Postives = 259/312 (83.01%), Query Frame = 0


Query: 1   MDASK-EPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALY 60
           M+ASK EPK ++K P  + RRKWTK+DK  AC L  +H+LCI APFQFNWSAFW+AF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  IITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTE 120
           I+TGLFG+T+SYHRNL+H+SFKLPKWLEYLFAYCGVQ+LQGDP+ WVSTHR HH+FVDTE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 KDPHSPIQGFWFSYIFWIFDSNTLTNKM-----SNFQERPDRSTSVMIMKHNRLDNVGDL 180
           +DPHSPI+GFWFS+I W+FDS  LT K+     SNFQ + +R+  V+  KH + +NV DL
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQ-KLERNIFVLFSKHGKPENVADL 180

Query: 181 KKQTFYTFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGK 240
           +KQ FY FIH TY+LHPI LAVILY  GGIPFL+WGM VR V++LH T +VNS+CH+WGK
Sbjct: 181 EKQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGK 240

Query: 241 QPWKTGDLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLA 300
           Q W TGD+S+NNW ++LI FGEGWHNNHHAFEYSA+ G EWWQ D GWY+++FLQAIG+A
Sbjct: 241 QSWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVA 300

Query: 301 TQVKLPSQSHKQ 307
           T VKLPSQSH Q
Sbjct: 301 THVKLPSQSHMQ 311

[Close]

BLAST of MS016276 vs. ExPASy TrEMBL
Match: A0A6J1GWB1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458011 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.9e-131
Identity = 217/309 (70.23%), Postives = 247/309 (79.94%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDASKEPK IVK PMSFG++KWTKIDK  A    FLH LCIFAPFQ NWSA  I+F LYI
Sbjct: 1   MDASKEPKSIVKPPMSFGKKKWTKIDKKVAFTFFFLHFLCIFAPFQINWSAISISFTLYI 60

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           I GLFGITISYHRNLSH+SFK+PKWLEYLFAYCGV + QGDPI WVSTHRYHH+FVDTEK
Sbjct: 61  IIGLFGITISYHRNLSHKSFKIPKWLEYLFAYCGVHAFQGDPINWVSTHRYHHQFVDTEK 120

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPI+GFW S+I W+               + D S S M+ +H + DN  DL+ QTFY
Sbjct: 121 DPHSPIKGFWSSHIIWLL-------------YKGDGSLSEMLGRHRKWDNACDLENQTFY 180

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
            FIH TY LHPIALA+ILY  GG P+ IWGMCVRSV+ LH T++VNS+CH+WGKQ WKT 
Sbjct: 181 RFIHKTYFLHPIALALILYGIGGFPYFIWGMCVRSVIFLHGTFMVNSVCHIWGKQQWKTN 240

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNW I+L+ FGEGWHNNHHAFEYSAR+G EWWQ+DPGWYV+ FL+AIG+AT VKLP
Sbjct: 241 DLSRNNWWISLLVFGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLP 296

Query: 301 SQSHKQKMA 310
           SQ H QK+A
Sbjct: 301 SQRHMQKLA 296

[Close]

BLAST of MS016276 vs. ExPASy TrEMBL
Match: A0A6J1GV97 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457793 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.0e-131
Identity = 214/309 (69.26%), Postives = 251/309 (81.23%), Query Frame = 0


Query: 1   MDASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYI 60
           MDAS+EPK IVK PMS G+RKW K+DK  A  L+FLH LCIFAP Q N+ A  I F L+I
Sbjct: 1   MDASREPKTIVKPPMSLGKRKWNKMDKKLAFALVFLHFLCIFAPLQINFGALSICFCLFI 60

Query: 61  ITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEK 120
           ITGLFGIT+SYHRNLSH+SFK+PKWLEY+FAYCGV +LQGDPIYWVSTHRYHH+FVDTE+
Sbjct: 61  ITGLFGITLSYHRNLSHKSFKVPKWLEYVFAYCGVHALQGDPIYWVSTHRYHHQFVDTEQ 120

Query: 121 DPHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFY 180
           DPHSPI+GFWFS++ W             F  + D S S M+ +H +LDN  DL+ QTFY
Sbjct: 121 DPHSPIKGFWFSHLIW-------------FLHKWDGSASEMVARHEKLDNARDLENQTFY 180

Query: 181 TFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTG 240
            FI  TY LH I LA+ILY  GGIP+ IWG CVR+VM+LH+T++VNSICH+WGKQPWKT 
Sbjct: 181 KFIRKTYFLHSIVLAIILYGIGGIPYFIWGTCVRTVMILHSTFMVNSICHIWGKQPWKTN 240

Query: 241 DLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLP 300
           DLSRNNW ++L+AFGEGWHNNHHAFEYSAR+G EWWQFDPGWYV++FLQAIG+AT VKLP
Sbjct: 241 DLSRNNWWMSLLAFGEGWHNNHHAFEYSARLGIEWWQFDPGWYVIVFLQAIGVATHVKLP 296

Query: 301 SQSHKQKMA 310
           SQS  QK+A
Sbjct: 301 SQSQMQKLA 296

[Close]

BLAST of MS016276 vs. ExPASy TrEMBL
Match: A0A6J1DHZ7 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021091 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.2e-130
Identity = 217/316 (68.67%), Postives = 261/316 (82.59%), Query Frame = 0


Query: 1   MDASK-EPKIIVKSPMSFG-RRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFAL 60
           M+A K EPK +   P SFG R+KWTK+DK  AC L F+H+L +FAPF FNW AFWIAFAL
Sbjct: 21  MEAPKQEPKEMAIPPKSFGKRKKWTKLDKDVACSLFFVHLLSMFAPFYFNWRAFWIAFAL 80

Query: 61  YIITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDT 120
           Y+ TGLFGI++SYHRNL+H+SF LPKWLEYLFAYCG  +LQGDPI WV+THR HHRFVDT
Sbjct: 81  YVATGLFGISVSYHRNLAHKSFTLPKWLEYLFAYCGAHALQGDPIDWVNTHRCHHRFVDT 140

Query: 121 EKDPHSPIQGFWFSYIFWIFDSNTLTNKM-----SNFQERPDRSTSVMIMKHNRLDNVGD 180
           E+DPHSPIQGFWFS+I W+ DS +LT ++     ++FQ + +R+  VM  KH R DNVGD
Sbjct: 141 ERDPHSPIQGFWFSHITWLLDSYSLTKRVCPKYFTDFQ-KVERNIFVMFSKHGRPDNVGD 200

Query: 181 LKKQTFYTFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWG 240
           L+KQTFY FIH TY LHPI LA+ILYA GG PFLIWGM VR V++LH T ++NS+CH+WG
Sbjct: 201 LEKQTFYRFIHKTYFLHPIILAIILYAYGGAPFLIWGMGVRIVVVLHITCMINSVCHIWG 260

Query: 241 KQPWKTGDLSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGL 300
           KQPW+TGDLSRNNW ++L++FGEGWHNNHHAFEYSAR G EWWQ D GWYVV+FL+AIG+
Sbjct: 261 KQPWRTGDLSRNNWWVSLVSFGEGWHNNHHAFEYSARHGIEWWQIDFGWYVVMFLEAIGI 320

Query: 301 ATQVKLPSQSHKQKMA 310
           AT VK+PSQ+ KQK+A
Sbjct: 321 ATNVKVPSQNQKQKLA 335

[Close]

BLAST of MS016276 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 402.1 bits (1032), Expect = 3.9e-112
Identity = 180/298 (60.40%), Postives = 224/298 (75.17%), Query Frame = 0


Query: 12  KSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISY 71
           K  + +  R+W  +D     ++L +H+L + APFQFNW A  +AF LYI+TGL GIT+S+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 72  HRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWF 131
           HRNLSH++FKLPKWLEYLFAYCG Q+LQG+PI WVSTHRYHH+F D+++DPHSP+ GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 132 SYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHP 191
           S++ W+FD+NT+T +                      +NVGDL+KQ FY F+  TYILHP
Sbjct: 208 SHMNWMFDTNTITQRC------------------GEPNNVGDLEKQPFYRFLRTTYILHP 267

Query: 192 IALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINL 251
           +ALAV LYA GG PF++WGM VR V + H T+LVNS CH+WGKQ W TGDLS+NNW +  
Sbjct: 268 LALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAA 327

Query: 252 IAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           +AFGEGWHNNHHAFE+SAR G EWWQ D  WYVV FLQAIGLAT VKLPS++ KQ+MA
Sbjct: 328 LAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

[Close]

BLAST of MS016276 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 344.4 bits (882), Expect = 9.8e-95
Identity = 153/290 (52.76%), Postives = 198/290 (68.28%), Query Frame = 0


Query: 20  RKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLSHRS 79
           RKW ++D   A   LF+H LC+ APF F W A  +A  +Y + GL GIT+SYHRNL+HRS
Sbjct: 32  RKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHRNLAHRS 91

Query: 80  FKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFWIFD 139
           FK+PKWLEY FAYCG+ ++QGDPI WVSTHRYHH+F D+++DPHSP +GFWFS++ W+FD
Sbjct: 92  FKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFD 151

Query: 140 SNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAVILY 199
                             T  ++ K  R  NV DLK+Q +Y F+  T + H +    +LY
Sbjct: 152 ------------------TGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLY 211

Query: 200 AGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGEGWH 259
             GG+ FL WGM +   M  H T L+NS+CH+WG + WKT D SRN W +++ +FGE WH
Sbjct: 212 YFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWH 271

Query: 260 NNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
           NNHHAFE SAR G EWWQ D  WY+V FL+ IGLAT VKLPS+S +++MA
Sbjct: 272 NNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 302

[Close]

BLAST of MS016276 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 335.5 bits (859), Expect = 4.5e-92
Identity = 148/293 (50.51%), Postives = 197/293 (67.24%), Query Frame = 0


Query: 17  FGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGITISYHRNLS 76
           F  R+W ++D         +H L + APF F WSA W+ F  Y I GL GIT+SYHRNL+
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLA 90

Query: 77  HRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQGFWFSYIFW 136
           HRSFK+PKWLEYL AYC + ++QGDPI WVSTHRYHH+F D+E+DPHSP +GFWFS++ W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 137 IFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYILHPIALAV 196
           I+DS                  + ++ K  R  NV DLK+Q FY F+  T + H + L  
Sbjct: 151 IYDS------------------AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGF 210

Query: 197 ILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWLINLIAFGE 256
            L+  GG+ F+ WGM V + + +H T L+NS+CH+WG + WKT D SRN W +++ +FGE
Sbjct: 211 FLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGE 270

Query: 257 GWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKMA 310
            WHNNHHAFE SAR G EWWQ D  WY+V F + IGLAT VK+P+++ +++MA
Sbjct: 271 SWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 304

[Close]

BLAST of MS016276 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.5e-90
Identity = 150/301 (49.83%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 9   IIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYIITGLFGIT 68
           ++V++  SF  R WT  D +   I +  H+L + APF F+W AFW+   L  I G+  IT
Sbjct: 78  VVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-IT 137

Query: 69  ISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKDPHSPIQG 128
           +SYHRNLSHRSF LPKWLEYLFAY GV + QGDPI WVS HRYHH+  +T++DPHSP QG
Sbjct: 138 LSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQG 197

Query: 129 FWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYTFIHNTYI 188
           FWFS++ WIFD                  TS ++      +NV DL +Q FY F+  T +
Sbjct: 198 FWFSHMAWIFD------------------TSSILENCGGEENVDDLVRQPFYRFLQRTVL 257

Query: 189 LHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGDLSRNNWL 248
           LH +A + + Y  GG+P L+WG+ +   + LH T+LVNS+CH+WG + W T D S+NNW 
Sbjct: 258 LHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWW 317

Query: 249 INLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPSQSHKQKM 308
           + ++  GEGWHNNHHAFE+SAR G EWWQ D  W ++ FL+AIGLAT VKLP+++  +  
Sbjct: 318 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGK 359

Query: 309 A 310
           A
Sbjct: 378 A 359

[Close]

BLAST of MS016276 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.0e-88
Identity = 150/308 (48.70%), Postives = 199/308 (64.61%), Query Frame = 0


Query: 2   DASKEPKIIVKSPMSFGRRKWTKIDKTNACILLFLHVLCIFAPFQFNWSAFWIAFALYII 61
           D+S + K + K   +F  RKWT++D      +  +H+LC+ APF + W AF  A  + I 
Sbjct: 8   DSSSQSKAVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGIS 67

Query: 62  TGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHRFVDTEKD 121
           T L  IT SYHRNL+HRSFKLPKWLEY FAY  + +LQG PI WVSTHR+HH+F D+++D
Sbjct: 68  TNL-SITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRD 127

Query: 122 PHSPIQGFWFSYIFWIFDSNTLTNKMSNFQERPDRSTSVMIMKHNRLDNVGDLKKQTFYT 181
           PHSPI+GFWFS++FWIFD++ +  K                      DNV DLK+Q FY 
Sbjct: 128 PHSPIEGFWFSHVFWIFDTSYIREKCGG------------------RDNVMDLKQQWFYR 187

Query: 182 FIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHLWGKQPWKTGD 241
           F+ NT  LH +   +++Y  GG+P+L W + V   +  HAT+L+NS CH+WG + W T D
Sbjct: 188 FLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWNTKD 247

Query: 242 LSRNNWLINLIAFGEGWHNNHHAFEYSARMGFEWWQFDPGWYVVIFLQAIGLATQVKLPS 301
            SRN W +     GE WHNNHHAFE SAR G EW+Q D  WY++ F Q +GLAT VKLP+
Sbjct: 248 TSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATDVKLPT 296

Query: 302 QSHKQKMA 310
            + K+KM+
Sbjct: 308 DAQKRKMS 296

The following BLAST results are available for this feature:

BLAST of MS016276 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153399.1	3.3e-185	98.71	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022153644.1	1.2e-131	68.91	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M...	[more]
XP_022956258.1	6.1e-131	70.23	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_022955961.1	1.0e-130	69.26	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]
XP_023526904.1	1.4e-130	69.90	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]

BLAST of MS016276 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	5.6e-111	60.40	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
P0DOW2	2.3e-101	54.48	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
P0DOW3	7.5e-100	55.17	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]
Q9FV68	7.3e-95	52.82	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]
O65797	1.4e-93	52.76	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]

BLAST of MS016276 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIY2	1.6e-185	98.71	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DJP0	5.9e-132	68.91	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GWB1	2.9e-131	70.23	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1GV97	5.0e-131	69.26	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1DHZ7	4.2e-130	68.67	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of MS016276 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	3.9e-112	60.40	fatty acid desaturase 5	[more]
AT1G06080.1	9.8e-95	52.76	delta 9 desaturase 1	[more]
AT2G31360.1	4.5e-92	50.51	16:0delta9 desaturase 2	[more]
AT3G15870.1	2.5e-90	49.83	Fatty acid desaturase family protein	[more]
AT1G06120.1	1.0e-88	48.70	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 255..269 score: 60.7 coord: 109..138 score: 45.61 coord: 72..92 score: 35.59
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 19..309
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 49..298 e-value: 2.60038E-71 score: 216.653
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 52..282 e-value: 3.3E-17 score: 63.2
None	No IPR available	PANTHER	PTHR11351:SF94	BNAC05G37460D PROTEIN	coord: 19..309

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016276.1	MS016276.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water