MS016130 (gene) Bitter gourd (TR) v1

Overview
NameMS016130
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold9_2: 572648 .. 576158 (+)
RNA-Seq ExpressionMS016130
SyntenyMS016130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCGTCTGGTCTTGAAGGTCGCTCAATTGATGTTGGCAATATTAAGATTCATGTTAGAAACGTCATTGCTGAAGGAGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTGTACACCTTTCAAAACAGTATGCTTTAAAGCACATTATATGCAATGATGAGGAATCACTTGAATTGGTGTTGAAGGAGGTTTCTGTAATGAAATCGCTCCGTGGCCACCCCAATGTTGTTACGCTTTATGCCCATACTATCATAGATATGGGACGCACGAAGGAAGCTCTACTTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCCTGGAGAGTAGAGGAGCTGGGTACTTTGATGAAAAGCAAGTTCTCTTAATTTTCAGAGACGTGTGCAATGCAGTATTTTCCATGCATTGCCATTCCCCACCAATTGCTCACAGGTAATCATGGTAGCTAATTATTTATATTGAAGCAATATTTTGCTCTTCTAAGTTATAATAGTTTCCACATAATTCTTGATTTTGACGTTTTCCATGCCTAGCCAATGAATTGACATGATTGTCTCAGCTAGTAGTTTGGTTGTTAACTTGTAGTTCTGTAGTTTTGTAATTAAGAAACAAAAAGATCTTGTTCAGTAGCTTTGAAGTCATGTCTACACTCTTATATATTTTTTGTGGTATATGGTTTTTGTTTATCTAATCCAACCATGTTTGGAAGCACTTGTAACTGCTATATGGTTTTTGTATTGCTATTCTTTTATAAGGTTATGGTATGATCCGAGTTTTTTGTTAATGAATCAACAAAGATTCTAATTTTTTATTGCTATTCTTTTATAAGATTATTAATTATTAATTTTCCCCCTACTGCAATTTTCAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCTCTGGAAATTGTGTGATTTTGGAAGCACTTCTACAAATCACAAGCGCTTTGAGAAGCCCGAGGAGATGGGCATTGAAGAAGATAATATCAGGAAGTACACTACACCTGCCTATAGGGCCCCTGAGGTATCAAAAAGTTCCATATCTTATAAATAACTTGCAGTTGTGATATTTGGTTGTAAATATGGTATTAATTTTTGTAAATTTTCTGATATGATGCAGATGTGGGATCTATTCCGTAGAGAACTTATAAATGAGAAAGTTGACATATGGGTATGTGAAGTATTTCTTTTCCTCAATATTCAAAATATTAAGTTACCATTAATCTCAATAGTTGGTGTAACCGAAAGAGCTTGTAGCTTGTTGGCTTAGTGGATAGACTATTCGTAGGCCCTGGTTCGAGTCTTGAAAAAAATAGTTGTAATTGTGAGACAGAGCTCATGTTATATTTATATCAATTCTTAACACCCTACTCCACACATGAGCTCAACTCTACTTTGTCAAGGTATGAGGTGGCTTACCATGGTTACTGTTGATCTTGAAGTGGGGAAAATATGATAATGCATGCTACCAGAGCACAACACTTCAACTCTGGAAGCTTTAAACGAACTGCTATAATTTAAGCTACAAGTTGAGGATAATTTATTTATCCCATAAATTTTATACAGATTAATCATAGATTCTGATTTCTGAGTAATTTTAAATGATTGGTGTTCTTTTCATAATGGAGATTTAAATTCTGTTACGTCGCAGGCTCTTGGGTGTCTTCTCTTCCGTATATGTTACTTCAAAAGCGCTTTTGATGGAGAGTCAAAACTTCAAATTTTAAATGGGAATTATCGAATTCCGGAGTTACCTAAATATAGCCCATCCATTACCGACCTAATCAGAGATATGCTCCAAGGATCCCCAAATGACAGACCAGACATCACGCAGGCAAGCGCTTTGCTTGATTGGCCATTCATCTCCATGAATTTAGGTTAGTTTAGATTGCTCATGTCCATGACAATAATAGGTGTCAGGATTCTCATAGTTTTCCTTCCTAATCTTGCGGTTGGTTTGTGCTGTGGCAGGTCTGGTTTCGTGTTAATGGCCTACTACCTGTTGATTCACAGAAATCATTACCTGATCAGCCACCTGACATGCCCCCTACAGACAAACTGGAAGGTGAGTGTGAAGAGTACCTTTAGCTTTAGTGTTCACTCGTTTTAATAGATATCTTTTCCTAAAGAAAAATTTGAATGAATATTAGATAGGATGCATGTGGGTTGGAATCAAGTAAACTATTATGCTCCAGCAGCATTTACTTGTTATATGCTCTTTAGTTCATTGTGAAAGCAGATGTCGTTTATAGGGTGGGAGAAATATGTGTGACGGGTTTAAATTATGATTTGGTGTGGAAGTTTGTTGATGTAAATGATATTCTTAGCAGGCATTTCAAATACCATGAACAAGTCACCTCCCATGCCTCGTCGAAGTCCACCCCCACCTCCATCAGTTAAAAGTTCATCACAAGCAGCACATGTATCTAAGGCAGCAGGGGCAGGTGGAGGTGGAGGACCGATGGGTGCCTTCTGGTCTACTCAACATGCCAATGACACCACCATCAAAGAGGATGCAACCAGAATTAGGTTTGATGAAGAATCGACTTCTCGTAGTACATCTAAAGCCGATAAGGTTAATCCAAATAATCACTCTACGCGTAAGAATGCCAGTCCTGGGGATATAAACCAAAAGTCAAACAAGACAGATAGTGGTTCATTCAGAGACTTCGAATTATCATTTTTCCAGAACGAAACGGAATGTGGTTCTGAAGAATCCAAAGGATCAAAAACTGAGAGTTCAGCTTTCCAGGACAAAGCTTTTAACAATTTTGTTGCTGAGTTTGGTACTGCCAATTTCAGTTCTGATGTCACTAACAACAGATCAGGAAAGGAAGTAGCTTTAGAGGCTGAGGTGGAGAAGTTGAAGGAACAACTGAAGCAAGCAAATGTGGAGAAAACTGAAATCACATCCAAATTTGAAAAGCTGTCTGCTATCTGCCGTTCTCAGAGGCAAGAGATACAGGAGCTCAAGCAAGCACTTGCTGCTAGGAGTCCATCACCAAACAAAGTTGAAATGAAAAACCATAACTCCCGGGACATTCCGCCTTCCACTATGCCGCGGGTATTTCCCTGCTTTTTTCATTTCTTTTTCGTCCTGTGAATATCTTTGTAACATAAAATGATTAGTGTTAATTCCACATTTCAGCTACAAAAGGCCGATGTGAATGCTCAAAGCCCAGATGCAAAGCAATGGCAGGCATTTGCAGAAGAATCCCCGAACCAGCAGCCTGTTAATCCGGAAAGGGGTGTAAAATCTATGAGGACCAGAAGCCTCCAGAGAAAGCAGGCTGCTCCAGAAGACACCGGTTTTGAAAGCTGGGGCTTCGGAGCGGAAAGTTTCACATCCGTTTCTGCTGGAAGCTCCAACTCATCAGGATTAGCTGGTGAAAAGAGTAGCTCACAACAACGTATTGGTGAACCCAGAAACACTGACATATCTTCTCAACCTGCTGGATGGGCTGGTTTC

mRNA sequence

ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCGTCTGGTCTTGAAGGTCGCTCAATTGATGTTGGCAATATTAAGATTCATGTTAGAAACGTCATTGCTGAAGGAGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTGTACACCTTTCAAAACAGTATGCTTTAAAGCACATTATATGCAATGATGAGGAATCACTTGAATTGGTGTTGAAGGAGGTTTCTGTAATGAAATCGCTCCGTGGCCACCCCAATGTTGTTACGCTTTATGCCCATACTATCATAGATATGGGACGCACGAAGGAAGCTCTACTTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCCTGGAGAGTAGAGGAGCTGGGTACTTTGATGAAAAGCAAGTTCTCTTAATTTTCAGAGACGTGTGCAATGCAGTATTTTCCATGCATTGCCATTCCCCACCAATTGCTCACAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCTCTGGAAATTGTGTGATTTTGGAAGCACTTCTACAAATCACAAGCGCTTTGAGAAGCCCGAGGAGATGGGCATTGAAGAAGATAATATCAGGAAGTACACTACACCTGCCTATAGGGCCCCTGAGATGTGGGATCTATTCCGTAGAGAACTTATAAATGAGAAAGTTGACATATGGGCTCTTGGGTGTCTTCTCTTCCGTATATGTTACTTCAAAAGCGCTTTTGATGGAGAGTCAAAACTTCAAATTTTAAATGGGAATTATCGAATTCCGGAGTTACCTAAATATAGCCCATCCATTACCGACCTAATCAGAGATATGCTCCAAGGATCCCCAAATGACAGACCAGACATCACGCAGGTCTGGTTTCGTGTTAATGGCCTACTACCTGTTGATTCACAGAAATCATTACCTGATCAGCCACCTGACATGCCCCCTACAGACAAACTGGAAGCAGGCATTTCAAATACCATGAACAAGTCACCTCCCATGCCTCGTCGAAGTCCACCCCCACCTCCATCAGTTAAAAGTTCATCACAAGCAGCACATGTATCTAAGGCAGCAGGGGCAGGTGGAGGTGGAGGACCGATGGGTGCCTTCTGGTCTACTCAACATGCCAATGACACCACCATCAAAGAGGATGCAACCAGAATTAGGTTTGATGAAGAATCGACTTCTCGTAGTACATCTAAAGCCGATAAGGTTAATCCAAATAATCACTCTACGCGTAAGAATGCCAGTCCTGGGGATATAAACCAAAAGTCAAACAAGACAGATAGTGGTTCATTCAGAGACTTCGAATTATCATTTTTCCAGAACGAAACGGAATGTGGTTCTGAAGAATCCAAAGGATCAAAAACTGAGAGTTCAGCTTTCCAGGACAAAGCTTTTAACAATTTTGTTGCTGAGTTTGGTACTGCCAATTTCAGTTCTGATGTCACTAACAACAGATCAGGAAAGGAAGTAGCTTTAGAGGCTGAGGTGGAGAAGTTGAAGGAACAACTGAAGCAAGCAAATGTGGAGAAAACTGAAATCACATCCAAATTTGAAAAGCTGTCTGCTATCTGCCGTTCTCAGAGGCAAGAGATACAGGAGCTCAAGCAAGCACTTGCTGCTAGGAGTCCATCACCAAACAAAGTTGAAATGAAAAACCATAACTCCCGGGACATTCCGCCTTCCACTATGCCGCGGCTACAAAAGGCCGATGTGAATGCTCAAAGCCCAGATGCAAAGCAATGGCAGGCATTTGCAGAAGAATCCCCGAACCAGCAGCCTGTTAATCCGGAAAGGGGTGTAAAATCTATGAGGACCAGAAGCCTCCAGAGAAAGCAGGCTGCTCCAGAAGACACCGGTTTTGAAAGCTGGGGCTTCGGAGCGGAAAGTTTCACATCCGTTTCTGCTGGAAGCTCCAACTCATCAGGATTAGCTGGTGAAAAGAGTAGCTCACAACAACGTATTGGTGAACCCAGAAACACTGACATATCTTCTCAACCTGCTGGATGGGCTGGTTTC

Coding sequence (CDS)

ATGTGGAGGTTCAAGCCTTTCATGCACAAAGAACCGTCTGGTCTTGAAGGTCGCTCAATTGATGTTGGCAATATTAAGATTCATGTTAGAAACGTCATTGCTGAAGGAGGATTCTCTTGTGTGTACTTAGCCAAGGATGCTGTACACCTTTCAAAACAGTATGCTTTAAAGCACATTATATGCAATGATGAGGAATCACTTGAATTGGTGTTGAAGGAGGTTTCTGTAATGAAATCGCTCCGTGGCCACCCCAATGTTGTTACGCTTTATGCCCATACTATCATAGATATGGGACGCACGAAGGAAGCTCTACTTGTGATGGAATTTTGTGAGAAGTCTTTGGTTAATGTCCTGGAGAGTAGAGGAGCTGGGTACTTTGATGAAAAGCAAGTTCTCTTAATTTTCAGAGACGTGTGCAATGCAGTATTTTCCATGCATTGCCATTCCCCACCAATTGCTCACAGAGATTTGAAAGCTGAAAATCTTTTGTTAGGCTCAGATGGCCTCTGGAAATTGTGTGATTTTGGAAGCACTTCTACAAATCACAAGCGCTTTGAGAAGCCCGAGGAGATGGGCATTGAAGAAGATAATATCAGGAAGTACACTACACCTGCCTATAGGGCCCCTGAGATGTGGGATCTATTCCGTAGAGAACTTATAAATGAGAAAGTTGACATATGGGCTCTTGGGTGTCTTCTCTTCCGTATATGTTACTTCAAAAGCGCTTTTGATGGAGAGTCAAAACTTCAAATTTTAAATGGGAATTATCGAATTCCGGAGTTACCTAAATATAGCCCATCCATTACCGACCTAATCAGAGATATGCTCCAAGGATCCCCAAATGACAGACCAGACATCACGCAGGTCTGGTTTCGTGTTAATGGCCTACTACCTGTTGATTCACAGAAATCATTACCTGATCAGCCACCTGACATGCCCCCTACAGACAAACTGGAAGCAGGCATTTCAAATACCATGAACAAGTCACCTCCCATGCCTCGTCGAAGTCCACCCCCACCTCCATCAGTTAAAAGTTCATCACAAGCAGCACATGTATCTAAGGCAGCAGGGGCAGGTGGAGGTGGAGGACCGATGGGTGCCTTCTGGTCTACTCAACATGCCAATGACACCACCATCAAAGAGGATGCAACCAGAATTAGGTTTGATGAAGAATCGACTTCTCGTAGTACATCTAAAGCCGATAAGGTTAATCCAAATAATCACTCTACGCGTAAGAATGCCAGTCCTGGGGATATAAACCAAAAGTCAAACAAGACAGATAGTGGTTCATTCAGAGACTTCGAATTATCATTTTTCCAGAACGAAACGGAATGTGGTTCTGAAGAATCCAAAGGATCAAAAACTGAGAGTTCAGCTTTCCAGGACAAAGCTTTTAACAATTTTGTTGCTGAGTTTGGTACTGCCAATTTCAGTTCTGATGTCACTAACAACAGATCAGGAAAGGAAGTAGCTTTAGAGGCTGAGGTGGAGAAGTTGAAGGAACAACTGAAGCAAGCAAATGTGGAGAAAACTGAAATCACATCCAAATTTGAAAAGCTGTCTGCTATCTGCCGTTCTCAGAGGCAAGAGATACAGGAGCTCAAGCAAGCACTTGCTGCTAGGAGTCCATCACCAAACAAAGTTGAAATGAAAAACCATAACTCCCGGGACATTCCGCCTTCCACTATGCCGCGGCTACAAAAGGCCGATGTGAATGCTCAAAGCCCAGATGCAAAGCAATGGCAGGCATTTGCAGAAGAATCCCCGAACCAGCAGCCTGTTAATCCGGAAAGGGGTGTAAAATCTATGAGGACCAGAAGCCTCCAGAGAAAGCAGGCTGCTCCAGAAGACACCGGTTTTGAAAGCTGGGGCTTCGGAGCGGAAAGTTTCACATCCGTTTCTGCTGGAAGCTCCAACTCATCAGGATTAGCTGGTGAAAAGAGTAGCTCACAACAACGTATTGGTGAACCCAGAAACACTGACATATCTTCTCAACCTGCTGGATGGGCTGGTTTC

Protein sequence

MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLESRGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVDSQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGGGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDINQKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFSSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRIGEPRNTDISSQPAGWAGF
Homology
BLAST of MS016130 vs. NCBI nr
Match: XP_022152645.1 (probable serine/threonine-protein kinase DDB_G0276461 isoform X1 [Momordica charantia])

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 659/673 (97.92%), Postives = 667/673 (99.11%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDEKQVLLIFRDVCNAVF+MHCHSPPIAHRDLKAEN+LLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDEKQVLLIFRDVCNAVFAMHCHSPPIAHRDLKAENVLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300

Query: 301 SQKSLPDQPPDMPPTDKLEAG-ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360
           SQKSLPDQPPDMPPTDKLEAG ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG
Sbjct: 301 SQKSLPDQPPDMPPTDKLEAGIISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDI 420
           GGGGP+GAFWSTQHANDTTI EDAT+IRFDEESTSRS SKADKVNPNNHSTRKNASPGDI
Sbjct: 361 GGGGPVGAFWSTQHANDTTIIEDATKIRFDEESTSRSASKADKVNPNNHSTRKNASPGDI 420

Query: 421 NQKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFSS 480
           NQKSNKTDSGSFRDFELSFFQNETE GSEESKGSK ESSAFQDKAFNNFVAEFGTANFSS
Sbjct: 421 NQKSNKTDSGSFRDFELSFFQNETERGSEESKGSKIESSAFQDKAFNNFVAEFGTANFSS 480

Query: 481 DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA 540
           DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA
Sbjct: 481 DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA 540

Query: 541 ARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPERG 600
           ARSPSPNKVEMKNHNSRD+PPSTMPRLQK DVNAQSPDAKQWQAFAEESPNQQPVNPE+G
Sbjct: 541 ARSPSPNKVEMKNHNSRDVPPSTMPRLQKTDVNAQSPDAKQWQAFAEESPNQQPVNPEKG 600

Query: 601 VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRIGEPRN 660
           VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSN+SGLAGE+SSSQQRIGEPRN
Sbjct: 601 VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNASGLAGERSSSQQRIGEPRN 660

Query: 661 TDISSQPAGWAGF 673
           TDISSQPAGWAGF
Sbjct: 661 TDISSQPAGWAGF 673

BLAST of MS016130 vs. NCBI nr
Match: XP_022152646.1 (probable serine/threonine-protein kinase DDB_G0276461 isoform X2 [Momordica charantia])

HSP 1 Score: 1293.5 bits (3346), Expect = 0.0e+00
Identity = 657/672 (97.77%), Postives = 665/672 (98.96%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDEKQVLLIFRDVCNAVF+MHCHSPPIAHRDLKAEN+LLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDEKQVLLIFRDVCNAVFAMHCHSPPIAHRDLKAENVLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG 360
           SQKSLPDQPPDMPPTDKLE  ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG
Sbjct: 301 SQKSLPDQPPDMPPTDKLEGIISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG 360

Query: 361 GGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDIN 420
           GGGP+GAFWSTQHANDTTI EDAT+IRFDEESTSRS SKADKVNPNNHSTRKNASPGDIN
Sbjct: 361 GGGPVGAFWSTQHANDTTIIEDATKIRFDEESTSRSASKADKVNPNNHSTRKNASPGDIN 420

Query: 421 QKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFSSD 480
           QKSNKTDSGSFRDFELSFFQNETE GSEESKGSK ESSAFQDKAFNNFVAEFGTANFSSD
Sbjct: 421 QKSNKTDSGSFRDFELSFFQNETERGSEESKGSKIESSAFQDKAFNNFVAEFGTANFSSD 480

Query: 481 VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA 540
           VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA
Sbjct: 481 VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA 540

Query: 541 RSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPERGV 600
           RSPSPNKVEMKNHNSRD+PPSTMPRLQK DVNAQSPDAKQWQAFAEESPNQQPVNPE+GV
Sbjct: 541 RSPSPNKVEMKNHNSRDVPPSTMPRLQKTDVNAQSPDAKQWQAFAEESPNQQPVNPEKGV 600

Query: 601 KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRIGEPRNT 660
           KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSN+SGLAGE+SSSQQRIGEPRNT
Sbjct: 601 KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNASGLAGERSSSQQRIGEPRNT 660

Query: 661 DISSQPAGWAGF 673
           DISSQPAGWAGF
Sbjct: 661 DISSQPAGWAGF 672

BLAST of MS016130 vs. NCBI nr
Match: XP_038904766.1 (probable serine/threonine-protein kinase DDB_G0276461 [Benincasa hispida] >XP_038904767.1 probable serine/threonine-protein kinase DDB_G0276461 [Benincasa hispida] >XP_038904768.1 probable serine/threonine-protein kinase DDB_G0276461 [Benincasa hispida])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 592/678 (87.32%), Postives = 627/678 (92.48%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFC+KSLVNVLES
Sbjct: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCDKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRD+CNAVF+MHCHSPPIAHRDLKAENLLLGSDG+WKLCDFGSTST
Sbjct: 121 RGAGYFDENQVLLIFRDICNAVFAMHCHSPPIAHRDLKAENLLLGSDGIWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS SITDLIRDMLQ SPNDRPDITQVWFR N LLPV 
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSITDLIRDMLQASPNDRPDITQVWFRANNLLPVG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAA--G 360
           SQKSLPDQPPDMP  DK E G SN MNKS PMPRRSPPPPPSVKSSSQA +H+ KAA  G
Sbjct: 301 SQKSLPDQPPDMPSADKQE-GTSNPMNKSSPMPRRSPPPPPSVKSSSQATSHMPKAAGGG 360

Query: 361 AGGGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPG 420
            GGGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D++NPNNHS  KNASPG
Sbjct: 361 GGGGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKQDRINPNNHSAHKNASPG 420

Query: 421 DINQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTA 480
           D+NQK NK  TDSGSFRDFELSFFQNE E GS  SK SKTES+ FQDKAFNNFVAEF TA
Sbjct: 421 DVNQKPNKTVTDSGSFRDFELSFFQNEAEHGSGGSKASKTESATFQDKAFNNFVAEFDTA 480

Query: 481 NFSSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELK 540
            FSS+ TNNR GK++ALEAEVEKLKEQLKQAN+E+ EITSKFEKLSAICRSQRQEIQELK
Sbjct: 481 KFSSEATNNRPGKKIALEAEVEKLKEQLKQANLERAEITSKFEKLSAICRSQRQEIQELK 540

Query: 541 QALAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVN 600
           QALAARSPSPNK E+KNHNS+D+  S +PRLQKAD++  SPDAK WQAFAEESPNQQ V+
Sbjct: 541 QALAARSPSPNKPEIKNHNSQDVQSSAVPRLQKADLSTPSPDAKPWQAFAEESPNQQSVS 600

Query: 601 PERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRI- 660
           PE+GVKS+RTRSLQRKQA+PED GFESWGFGAESFT+VSAGSSN+SGLAGE++SSQQR  
Sbjct: 601 PEKGVKSVRTRSLQRKQASPEDAGFESWGFGAESFTAVSAGSSNTSGLAGERNSSQQRTG 660

Query: 661 GEPRNTDISSQPAGWAGF 673
           GEP+N DISSQPAGWAGF
Sbjct: 661 GEPKNNDISSQPAGWAGF 676

BLAST of MS016130 vs. NCBI nr
Match: XP_004149621.1 (probable serine/threonine-protein kinase DDB_G0276461 [Cucumis sativus] >XP_011651798.1 probable serine/threonine-protein kinase DDB_G0276461 [Cucumis sativus] >KGN58620.1 hypothetical protein Csa_001781 [Cucumis sativus])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 589/678 (86.87%), Postives = 620/678 (91.45%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELV+KEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVMKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRDVCNAVF+MHCHSPP+AHRDLKAENLLLGSDG WKLCDFGSTST
Sbjct: 121 RGAGYFDESQVLLIFRDVCNAVFAMHCHSPPVAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS S+ DLIRDMLQ SPNDRPDITQVWFR N LLPV 
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSVIDLIRDMLQASPNDRPDITQVWFRANNLLPVG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAA--G 360
           SQKSLPDQPPDMP TDK E G SN +NK  PMPRRSPPPPPSVKSSSQA +++SK A  G
Sbjct: 301 SQKSLPDQPPDMPSTDKHE-GTSNPVNKLSPMPRRSPPPPPSVKSSSQATSNMSKPAGGG 360

Query: 361 AGGGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPG 420
            GGGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D+  PNNHST KNASPG
Sbjct: 361 GGGGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKHDRNGPNNHSTHKNASPG 420

Query: 421 DINQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTA 480
           D+NQK NK  TDSGSFRDFELSFFQNE E GS  SK SKT S+ FQDKAFNNFVAEF T 
Sbjct: 421 DVNQKGNKTVTDSGSFRDFELSFFQNEMEHGSSGSKASKTGSANFQDKAFNNFVAEFDTG 480

Query: 481 NFSSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELK 540
            FSSDVTNN+ GKEVALEAEVEKLKEQLK ANVEK+EITSKFEKLSAICRSQRQEIQELK
Sbjct: 481 KFSSDVTNNKPGKEVALEAEVEKLKEQLKHANVEKSEITSKFEKLSAICRSQRQEIQELK 540

Query: 541 QALAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVN 600
           QALAARSPSPNK+EMKN NSR+  PS +PRLQKADV   SPDAK WQAFAEESPNQQPV 
Sbjct: 541 QALAARSPSPNKLEMKNQNSREAQPSAVPRLQKADVTTPSPDAKAWQAFAEESPNQQPVT 600

Query: 601 PERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQR-I 660
           PE+GVKSMRTRS+QRKQA+PE+TGFESWGFGAESFT+VSAGSSN SGL GE++SSQ+   
Sbjct: 601 PEKGVKSMRTRSVQRKQASPENTGFESWGFGAESFTAVSAGSSNKSGLTGERNSSQRTGG 660

Query: 661 GEPRNTDISSQPAGWAGF 673
           GEP++ DISSQPAGWAGF
Sbjct: 661 GEPKSNDISSQPAGWAGF 676

BLAST of MS016130 vs. NCBI nr
Match: XP_008461768.1 (PREDICTED: probable serine/threonine-protein kinase DDB_G0276461 [Cucumis melo] >KAA0048302.1 putative serine/threonine-protein kinase [Cucumis melo var. makuwa] >TYK10647.1 putative serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 583/676 (86.24%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELV+KEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALL MEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVMKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLAMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRD+CNAVF+MHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDENQVLLIFRDICNAVFAMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS S+TDLIRDMLQ SPNDRPDITQVWFR N LLP  
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSVTDLIRDMLQASPNDRPDITQVWFRANNLLPAG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAAGAG 360
           SQKSLPDQPPDMP TDK E G SN +NK  PMPRRSPPPPPSVKSSSQA +H+SK AG G
Sbjct: 301 SQKSLPDQPPDMPSTDKHE-GTSNPVNKLSPMPRRSPPPPPSVKSSSQATSHMSKPAGGG 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDI 420
           GGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D+ +PNNHST KNA+PGD+
Sbjct: 361 GGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKHDRNSPNNHSTHKNANPGDV 420

Query: 421 NQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANF 480
           NQ+ NK  TDSGSFRDFELSFFQNE E         KTES+ FQDKAFNNFVAEF T  F
Sbjct: 421 NQRGNKTVTDSGSFRDFELSFFQNEME---------KTESANFQDKAFNNFVAEFDTVKF 480

Query: 481 SSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQA 540
           SSDVTNN+ GKEVALEAEVEKLKEQLKQANVEK+EITSKFEKLSAICRSQRQEIQELKQA
Sbjct: 481 SSDVTNNKPGKEVALEAEVEKLKEQLKQANVEKSEITSKFEKLSAICRSQRQEIQELKQA 540

Query: 541 LAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPE 600
           LAARSPSPNK+EMKNHNSR+  PS MPRLQKADV   SPDAK WQAFAEESPNQQ V PE
Sbjct: 541 LAARSPSPNKLEMKNHNSREAQPSAMPRLQKADVTTPSPDAKAWQAFAEESPNQQSVTPE 600

Query: 601 RGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQR-IGE 660
           +GVKSMRTRSLQ+KQA+PE+TGFESWGFGAESF++VSAGSSN SGL GE++SSQ+   GE
Sbjct: 601 KGVKSMRTRSLQKKQASPENTGFESWGFGAESFSAVSAGSSNKSGLTGERNSSQRTGGGE 660

Query: 661 PRNTDISSQPAGWAGF 673
           P++ ++SSQPAGWAGF
Sbjct: 661 PKSNEVSSQPAGWAGF 665

BLAST of MS016130 vs. ExPASy Swiss-Prot
Match: F1SPM8 (AP2-associated protein kinase 1 OS=Sus scrofa OX=9823 GN=AAK1 PE=2 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 1.2e-57
Identity = 136/343 (39.65%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 13  SGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLELVLK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  +  + ALK +  N+E  L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGM--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EVSVMKSLRGHPNVVTLYAHTI--IDMGRTKEALLVMEFCE-KSLVNVLESRGAGYFDEK 132
           E+ +M+ L GH N+V     +I  +  G   E L++M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTES 151

Query: 133 QVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   G + LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGES 252
             G+   ED I+KYTT +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES
Sbjct: 212 TEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GES 271

Query: 253 KLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVDSQKSLPD 312
           ++ I +GN+ IP+  +YS  +  LIR ML+  P+ RPDI QV F    LL          
Sbjct: 272 QVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSFFSFKLL---------- 331

Query: 313 QPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAA 351
                    K E  + N  N   P+P + P P   VK+S  AA
Sbjct: 332 ---------KKECPVPNVQNS--PIPAKLPEP---VKASEAAA 344

BLAST of MS016130 vs. ExPASy Swiss-Prot
Match: Q2M2I8 (AP2-associated protein kinase 1 OS=Homo sapiens OX=9606 GN=AAK1 PE=1 SV=3)

HSP 1 Score: 225.3 bits (573), Expect = 2.0e-57
Identity = 136/343 (39.65%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 13  SGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLELVLK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  +  + ALK +  N+E  L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGM--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EVSVMKSLRGHPNVVTLYAHTI--IDMGRTKEALLVMEFCE-KSLVNVLESRGAGYFDEK 132
           E+ +M+ L GH N+V     +I  +  G   E L++M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   G + LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGES 252
             G+   ED I+KYTT +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES
Sbjct: 212 TEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GES 271

Query: 253 KLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVDSQKSLPD 312
           ++ I +GN+ IP+  +YS  +  LIR ML+  P+ RPDI QV +    LL          
Sbjct: 272 QVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLL---------- 331

Query: 313 QPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAA 351
                    K E  I N  N   P+P + P P   VK+S  AA
Sbjct: 332 ---------KKECPIPNVQNS--PIPAKLPEP---VKASEAAA 344

BLAST of MS016130 vs. ExPASy Swiss-Prot
Match: F1MH24 (AP2-associated protein kinase 1 OS=Bos taurus OX=9913 GN=AAK1 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 2.6e-57
Identity = 136/343 (39.65%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 13  SGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLELVLK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  +  + ALK +  N+E  L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGM--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EVSVMKSLRGHPNVVTLYAHTI--IDMGRTKEALLVMEFCE-KSLVNVLESRGAGYFDEK 132
           E+ +M+ L GH N+V     +I  +  G   E L++M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   G + LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGES 252
             G+   ED I+KYTT +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES
Sbjct: 212 TEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPF-GES 271

Query: 253 KLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVDSQKSLPD 312
           ++ I +GN+ IP+  +YS  +  LIR ML+  P+ RPDI QV +    LL          
Sbjct: 272 QVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLL---------- 331

Query: 313 QPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAA 351
                    K E  I N  N   P+P + P P   VK+S  AA
Sbjct: 332 ---------KKECPIPNVQNS--PIPTKLPEP---VKASEAAA 344

BLAST of MS016130 vs. ExPASy Swiss-Prot
Match: Q3UHJ0 (AP2-associated protein kinase 1 OS=Mus musculus OX=10090 GN=Aak1 PE=1 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 2.2e-56
Identity = 133/343 (38.78%), Postives = 197/343 (57.43%), Query Frame = 0

Query: 13  SGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLELVLK 72
           SG  GR   +G  ++ V  V+AEGGF+ V+L + +  +  + ALK +  N+E  L++  +
Sbjct: 32  SGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGV--KCALKRMFVNNEHDLQVCKR 91

Query: 73  EVSVMKSLRGHPNVVTLYAHTI--IDMGRTKEALLVMEFCE-KSLVNVLESRGAGYFDEK 132
           E+ +M+ L GH N+V     +I  +  G   E L++M+FC    +VN++  R    F E 
Sbjct: 92  EIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTEN 151

Query: 133 QVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPE 192
           +VL IF D C AV  +H    PI HRDLK EN+LL   G + LCDFGS +    +F+ P+
Sbjct: 152 EVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSAT---NKFQNPQ 211

Query: 193 EMGIE--EDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGES 252
             G+   ED I+KYTT +YRAPEM +L+  ++I  K DIWALGCLL+++CYF   F GES
Sbjct: 212 AEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPF-GES 271

Query: 253 KLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLL----PVDSQK 312
           ++ I +G++ IP+  +YS  +  LIR ML+  P+ RPDI QV +    LL    PV + +
Sbjct: 272 QVAICDGSFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQ 331

Query: 313 SLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSS 347
           + P       P    EA +  T  K+     R   P P+ ++S
Sbjct: 332 NSPIPAKLPEPVKASEAAVKKTQPKA-----RLTDPIPTTETS 363

BLAST of MS016130 vs. ExPASy Swiss-Prot
Match: O14976 (Cyclin-G-associated kinase OS=Homo sapiens OX=9606 GN=GAK PE=1 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 5.5e-55
Identity = 134/338 (39.64%), Postives = 193/338 (57.10%), Query Frame = 0

Query: 10  KEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHIICNDEESLEL 69
           ++ S   G+++++G +++ VR V+AEGGF+ VY A+D V   ++YALK ++ N+EE    
Sbjct: 23  RDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQD-VGSGREYALKRLLSNEEEKNRA 82

Query: 70  VLKEVSVMKSLRGHPNVVTLYAHTII-----DMGRTKEALLVMEFCEKSLVNVL---ESR 129
           +++EV  MK L GHPN+V   +   I     D G+  E LL+ E C+  LV  L   ESR
Sbjct: 83  IIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQA-EFLLLTELCKGQLVEFLKKMESR 142

Query: 130 GAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST- 189
           G    D   VL IF   C AV  MH   PPI HRDLK ENLLL + G  KLCDFGS +T 
Sbjct: 143 GPLSCD--TVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTI 202

Query: 190 -NHKRFE-KPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICY 249
            ++  +    +   + E+ I + TTP YR PE+ DL+    I EK DIWALGC+L+ +C+
Sbjct: 203 SHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCF 262

Query: 250 FKSAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLP 309
            +  F+  +KL+I+NG Y IP           LIR MLQ +P +R  I +V   V+ L  
Sbjct: 263 RQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEV---VHQLQE 322

Query: 310 VDSQKSLPDQPPDMPPTDKLEAG---ISNTMNKSPPMP 334
           + + +++    P  P T+ LE      S T+++ PP P
Sbjct: 323 IAAARNV---NPKSPITELLEQNGGYGSATLSRGPPPP 350

BLAST of MS016130 vs. ExPASy TrEMBL
Match: A0A6J1DEI5 (probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020313 PE=4 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 659/673 (97.92%), Postives = 667/673 (99.11%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDEKQVLLIFRDVCNAVF+MHCHSPPIAHRDLKAEN+LLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDEKQVLLIFRDVCNAVFAMHCHSPPIAHRDLKAENVLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300

Query: 301 SQKSLPDQPPDMPPTDKLEAG-ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360
           SQKSLPDQPPDMPPTDKLEAG ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG
Sbjct: 301 SQKSLPDQPPDMPPTDKLEAGIISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDI 420
           GGGGP+GAFWSTQHANDTTI EDAT+IRFDEESTSRS SKADKVNPNNHSTRKNASPGDI
Sbjct: 361 GGGGPVGAFWSTQHANDTTIIEDATKIRFDEESTSRSASKADKVNPNNHSTRKNASPGDI 420

Query: 421 NQKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFSS 480
           NQKSNKTDSGSFRDFELSFFQNETE GSEESKGSK ESSAFQDKAFNNFVAEFGTANFSS
Sbjct: 421 NQKSNKTDSGSFRDFELSFFQNETERGSEESKGSKIESSAFQDKAFNNFVAEFGTANFSS 480

Query: 481 DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA 540
           DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA
Sbjct: 481 DVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALA 540

Query: 541 ARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPERG 600
           ARSPSPNKVEMKNHNSRD+PPSTMPRLQK DVNAQSPDAKQWQAFAEESPNQQPVNPE+G
Sbjct: 541 ARSPSPNKVEMKNHNSRDVPPSTMPRLQKTDVNAQSPDAKQWQAFAEESPNQQPVNPEKG 600

Query: 601 VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRIGEPRN 660
           VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSN+SGLAGE+SSSQQRIGEPRN
Sbjct: 601 VKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNASGLAGERSSSQQRIGEPRN 660

Query: 661 TDISSQPAGWAGF 673
           TDISSQPAGWAGF
Sbjct: 661 TDISSQPAGWAGF 673

BLAST of MS016130 vs. ExPASy TrEMBL
Match: A0A6J1DID0 (probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020313 PE=4 SV=1)

HSP 1 Score: 1293.5 bits (3346), Expect = 0.0e+00
Identity = 657/672 (97.77%), Postives = 665/672 (98.96%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDEKQVLLIFRDVCNAVF+MHCHSPPIAHRDLKAEN+LLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDEKQVLLIFRDVCNAVFAMHCHSPPIAHRDLKAENVLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG 360
           SQKSLPDQPPDMPPTDKLE  ISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG
Sbjct: 301 SQKSLPDQPPDMPPTDKLEGIISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAGG 360

Query: 361 GGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDIN 420
           GGGP+GAFWSTQHANDTTI EDAT+IRFDEESTSRS SKADKVNPNNHSTRKNASPGDIN
Sbjct: 361 GGGPVGAFWSTQHANDTTIIEDATKIRFDEESTSRSASKADKVNPNNHSTRKNASPGDIN 420

Query: 421 QKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFSSD 480
           QKSNKTDSGSFRDFELSFFQNETE GSEESKGSK ESSAFQDKAFNNFVAEFGTANFSSD
Sbjct: 421 QKSNKTDSGSFRDFELSFFQNETERGSEESKGSKIESSAFQDKAFNNFVAEFGTANFSSD 480

Query: 481 VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA 540
           VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA
Sbjct: 481 VTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQALAA 540

Query: 541 RSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPERGV 600
           RSPSPNKVEMKNHNSRD+PPSTMPRLQK DVNAQSPDAKQWQAFAEESPNQQPVNPE+GV
Sbjct: 541 RSPSPNKVEMKNHNSRDVPPSTMPRLQKTDVNAQSPDAKQWQAFAEESPNQQPVNPEKGV 600

Query: 601 KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQRIGEPRNT 660
           KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSN+SGLAGE+SSSQQRIGEPRNT
Sbjct: 601 KSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNASGLAGERSSSQQRIGEPRNT 660

Query: 661 DISSQPAGWAGF 673
           DISSQPAGWAGF
Sbjct: 661 DISSQPAGWAGF 672

BLAST of MS016130 vs. ExPASy TrEMBL
Match: A0A0A0L9J9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G702580 PE=4 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 589/678 (86.87%), Postives = 620/678 (91.45%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELV+KEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVMKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRDVCNAVF+MHCHSPP+AHRDLKAENLLLGSDG WKLCDFGSTST
Sbjct: 121 RGAGYFDESQVLLIFRDVCNAVFAMHCHSPPVAHRDLKAENLLLGSDGHWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS S+ DLIRDMLQ SPNDRPDITQVWFR N LLPV 
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSVIDLIRDMLQASPNDRPDITQVWFRANNLLPVG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAA--G 360
           SQKSLPDQPPDMP TDK E G SN +NK  PMPRRSPPPPPSVKSSSQA +++SK A  G
Sbjct: 301 SQKSLPDQPPDMPSTDKHE-GTSNPVNKLSPMPRRSPPPPPSVKSSSQATSNMSKPAGGG 360

Query: 361 AGGGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPG 420
            GGGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D+  PNNHST KNASPG
Sbjct: 361 GGGGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKHDRNGPNNHSTHKNASPG 420

Query: 421 DINQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTA 480
           D+NQK NK  TDSGSFRDFELSFFQNE E GS  SK SKT S+ FQDKAFNNFVAEF T 
Sbjct: 421 DVNQKGNKTVTDSGSFRDFELSFFQNEMEHGSSGSKASKTGSANFQDKAFNNFVAEFDTG 480

Query: 481 NFSSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELK 540
            FSSDVTNN+ GKEVALEAEVEKLKEQLK ANVEK+EITSKFEKLSAICRSQRQEIQELK
Sbjct: 481 KFSSDVTNNKPGKEVALEAEVEKLKEQLKHANVEKSEITSKFEKLSAICRSQRQEIQELK 540

Query: 541 QALAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVN 600
           QALAARSPSPNK+EMKN NSR+  PS +PRLQKADV   SPDAK WQAFAEESPNQQPV 
Sbjct: 541 QALAARSPSPNKLEMKNQNSREAQPSAVPRLQKADVTTPSPDAKAWQAFAEESPNQQPVT 600

Query: 601 PERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQR-I 660
           PE+GVKSMRTRS+QRKQA+PE+TGFESWGFGAESFT+VSAGSSN SGL GE++SSQ+   
Sbjct: 601 PEKGVKSMRTRSVQRKQASPENTGFESWGFGAESFTAVSAGSSNKSGLTGERNSSQRTGG 660

Query: 661 GEPRNTDISSQPAGWAGF 673
           GEP++ DISSQPAGWAGF
Sbjct: 661 GEPKSNDISSQPAGWAGF 676

BLAST of MS016130 vs. ExPASy TrEMBL
Match: A0A5A7TXP2 (Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00090 PE=4 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 583/676 (86.24%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELV+KEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALL MEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVMKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLAMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRD+CNAVF+MHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDENQVLLIFRDICNAVFAMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS S+TDLIRDMLQ SPNDRPDITQVWFR N LLP  
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSVTDLIRDMLQASPNDRPDITQVWFRANNLLPAG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAAGAG 360
           SQKSLPDQPPDMP TDK E G SN +NK  PMPRRSPPPPPSVKSSSQA +H+SK AG G
Sbjct: 301 SQKSLPDQPPDMPSTDKHE-GTSNPVNKLSPMPRRSPPPPPSVKSSSQATSHMSKPAGGG 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDI 420
           GGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D+ +PNNHST KNA+PGD+
Sbjct: 361 GGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKHDRNSPNNHSTHKNANPGDV 420

Query: 421 NQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANF 480
           NQ+ NK  TDSGSFRDFELSFFQNE E         KTES+ FQDKAFNNFVAEF T  F
Sbjct: 421 NQRGNKTVTDSGSFRDFELSFFQNEME---------KTESANFQDKAFNNFVAEFDTVKF 480

Query: 481 SSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQA 540
           SSDVTNN+ GKEVALEAEVEKLKEQLKQANVEK+EITSKFEKLSAICRSQRQEIQELKQA
Sbjct: 481 SSDVTNNKPGKEVALEAEVEKLKEQLKQANVEKSEITSKFEKLSAICRSQRQEIQELKQA 540

Query: 541 LAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPE 600
           LAARSPSPNK+EMKNHNSR+  PS MPRLQKADV   SPDAK WQAFAEESPNQQ V PE
Sbjct: 541 LAARSPSPNKLEMKNHNSREAQPSAMPRLQKADVTTPSPDAKAWQAFAEESPNQQSVTPE 600

Query: 601 RGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQR-IGE 660
           +GVKSMRTRSLQ+KQA+PE+TGFESWGFGAESF++VSAGSSN SGL GE++SSQ+   GE
Sbjct: 601 KGVKSMRTRSLQKKQASPENTGFESWGFGAESFSAVSAGSSNKSGLTGERNSSQRTGGGE 660

Query: 661 PRNTDISSQPAGWAGF 673
           P++ ++SSQPAGWAGF
Sbjct: 661 PKSNEVSSQPAGWAGF 665

BLAST of MS016130 vs. ExPASy TrEMBL
Match: A0A1S3CFB6 (probable serine/threonine-protein kinase DDB_G0276461 OS=Cucumis melo OX=3656 GN=LOC103500292 PE=4 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 583/676 (86.24%), Postives = 618/676 (91.42%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MWRFKPFMHKEPSGLEGRSIDVGN+KIHVRNVIAEGGFSCVYLAKDAVH+SKQYALKHII
Sbjct: 1   MWRFKPFMHKEPSGLEGRSIDVGNLKIHVRNVIAEGGFSCVYLAKDAVHISKQYALKHII 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLVMEFCEKSLVNVLES 120
           CNDEESLELV+KEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALL MEFCEKSLVNVLES
Sbjct: 61  CNDEESLELVMKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEALLAMEFCEKSLVNVLES 120

Query: 121 RGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180
           RGAGYFDE QVLLIFRD+CNAVF+MHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST
Sbjct: 121 RGAGYFDENQVLLIFRDICNAVFAMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTST 180

Query: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240
           NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK
Sbjct: 181 NHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYFK 240

Query: 241 SAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPVD 300
           SAFDGESKLQILNGNYRIPELPKYS S+TDLIRDMLQ SPNDRPDITQVWFR N LLP  
Sbjct: 241 SAFDGESKLQILNGNYRIPELPKYSSSVTDLIRDMLQASPNDRPDITQVWFRANNLLPAG 300

Query: 301 SQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQA-AHVSKAAGAG 360
           SQKSLPDQPPDMP TDK E G SN +NK  PMPRRSPPPPPSVKSSSQA +H+SK AG G
Sbjct: 301 SQKSLPDQPPDMPSTDKHE-GTSNPVNKLSPMPRRSPPPPPSVKSSSQATSHMSKPAGGG 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRIRFDEESTSRSTSKADKVNPNNHSTRKNASPGDI 420
           GGGGP+GAFWSTQHA+D T+ ED  RIRFDEESTSRSTSK D+ +PNNHST KNA+PGD+
Sbjct: 361 GGGGPLGAFWSTQHASD-TVNEDTNRIRFDEESTSRSTSKHDRNSPNNHSTHKNANPGDV 420

Query: 421 NQKSNK--TDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANF 480
           NQ+ NK  TDSGSFRDFELSFFQNE E         KTES+ FQDKAFNNFVAEF T  F
Sbjct: 421 NQRGNKTVTDSGSFRDFELSFFQNEME---------KTESANFQDKAFNNFVAEFDTVKF 480

Query: 481 SSDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQA 540
           SSDVTNN+ GKEVALEAEVEKLKEQLKQANVEK+EITSKFEKLSAICRSQRQEIQELKQA
Sbjct: 481 SSDVTNNKPGKEVALEAEVEKLKEQLKQANVEKSEITSKFEKLSAICRSQRQEIQELKQA 540

Query: 541 LAARSPSPNKVEMKNHNSRDIPPSTMPRLQKADVNAQSPDAKQWQAFAEESPNQQPVNPE 600
           LAARSPSPNK+EMKNHNSR+  PS MPRLQKADV   SPDAK WQAFAEESPNQQ V PE
Sbjct: 541 LAARSPSPNKLEMKNHNSREAQPSAMPRLQKADVTTPSPDAKAWQAFAEESPNQQSVTPE 600

Query: 601 RGVKSMRTRSLQRKQAAPEDTGFESWGFGAESFTSVSAGSSNSSGLAGEKSSSQQR-IGE 660
           +GVKSMRTRSLQ+KQA+PE+TGFESWGFGAESF++VSAGSSN SGL GE++SSQ+   GE
Sbjct: 601 KGVKSMRTRSLQKKQASPENTGFESWGFGAESFSAVSAGSSNKSGLTGERNSSQRTGGGE 660

Query: 661 PRNTDISSQPAGWAGF 673
           P++ ++SSQPAGWAGF
Sbjct: 661 PKSNEVSSQPAGWAGF 665

BLAST of MS016130 vs. TAIR 10
Match: AT2G32850.2 (Protein kinase superfamily protein )

HSP 1 Score: 700.7 bits (1807), Expect = 1.2e-201
Identity = 398/705 (56.45%), Postives = 494/705 (70.07%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MW+FKPF  KEP+GLEGR +++GN+K+ VRNVIAEGGFS VYLA+D  H SKQYALKH+I
Sbjct: 1   MWKFKPFAQKEPAGLEGRFLEIGNLKVQVRNVIAEGGFSSVYLAQDVNHASKQYALKHMI 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRT-KEALLVMEFCEKSLVNVLE 120
           CNDEESLELV+KE+SV+KSL+GHPNVVTLYAH I+DMGR  KEALL M+FC KSLV+VLE
Sbjct: 61  CNDEESLELVMKEISVLKSLKGHPNVVTLYAHGILDMGRNKKEALLAMDFCGKSLVDVLE 120

Query: 121 SRGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTS 180
           +RGAGYF+EKQ L IFRDVCNAVF+MHC SP IAHRDLKAENLLL SDG WKLCDFGS S
Sbjct: 121 NRGAGYFEEKQALTIFRDVCNAVFAMHCQSPRIAHRDLKAENLLLSSDGQWKLCDFGSVS 180

Query: 181 TNHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYF 240
           TNHK FE+ EEMGIEEDNIRKYTTP YRAPEMWDLFRRE+I+EKVDIWALGCLLFRICYF
Sbjct: 181 TNHKIFERAEEMGIEEDNIRKYTTPTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYF 240

Query: 241 KSAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPV 300
           K+AFDGESKLQILNGNYRIPE PKYS  ITDLI++MLQ SP++RPDITQ+WFRVN  LP 
Sbjct: 241 KNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPA 300

Query: 301 DSQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360
           + QKSLPD+PP+M  T  +  G S +  K  P PRRSPPPPP             ++G  
Sbjct: 301 NLQKSLPDRPPEMQSTG-VHDGSSKSATKPSPAPRRSPPPPP------------PSSGES 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRI-RFDEESTSRSTSKADKVNPNNHSTRKNASPGD 420
             GGP+GAFW+TQHA  + + ED   + +FDE ++  +TSK+++V  ++H  +K   P  
Sbjct: 361 DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNS--NTSKSERVRVDSHQPKK---PSP 420

Query: 421 INQKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFS 480
           +  ++        +D E +  Q  T   +  +  + T  S  +D AFN+FVA+F T  F 
Sbjct: 421 VRGEARGIQRN--KDLETTISQKNT---TPAAANNMTRVS--KDDAFNSFVADFDTTKFD 480

Query: 481 SDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQAL 540
           +    N+ GKE ALEAE+++LK++LKQ   EK EIT+KFEKLSAICRSQRQE+Q+LKQ L
Sbjct: 481 N---GNKPGKEEALEAEIQRLKDELKQTKSEKAEITAKFEKLSAICRSQRQELQDLKQTL 540

Query: 541 AARS-------------PSPNKVEMKNHNSRDIPPSTMPRLQ--KADVNAQSPDAKQWQA 600
           A++S             PSP    M +  SRD    TM  LQ  +++ +  S D   WQ 
Sbjct: 541 ASKSASPSPSRDSSQNQPSPGMHSMSSTPSRDKMEGTMWELQQDRSNWSTGSSDTNSWQP 600

Query: 601 FAEESP---------NQQPVNPERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESF--TS 660
           F++E+          N   +N     +S+RTRS  +  +A    GFE WGF  ESF   +
Sbjct: 601 FSDEAKPVMESASKGNNNTIN-----QSVRTRS--KPASAAGTQGFEPWGFETESFRAAA 660

Query: 661 VSAGSSNSSGL---AGEKSSSQQRIG--EPRNTDISSQPAGWAGF 673
            SA ++++SG     G  +S+ QR G  + R    ++QPAGWAGF
Sbjct: 661 TSAAATSASGTQRSMGSGNSTSQRYGNSKMRENQKTAQPAGWAGF 670

BLAST of MS016130 vs. TAIR 10
Match: AT2G32850.1 (Protein kinase superfamily protein )

HSP 1 Score: 695.7 bits (1794), Expect = 3.8e-200
Identity = 393/696 (56.47%), Postives = 487/696 (69.97%), Query Frame = 0

Query: 1   MWRFKPFMHKEPSGLEGRSIDVGNIKIHVRNVIAEGGFSCVYLAKDAVHLSKQYALKHII 60
           MW+FKPF  KEP+GLEGR +++GN+K+ VRNVIAEGGFS VYLA+D  H SKQYALKH+I
Sbjct: 1   MWKFKPFAQKEPAGLEGRFLEIGNLKVQVRNVIAEGGFSSVYLAQDVNHASKQYALKHMI 60

Query: 61  CNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRT-KEALLVMEFCEKSLVNVLE 120
           CNDEESLELV+KE+SV+KSL+GHPNVVTLYAH I+DMGR  KEALL M+FC KSLV+VLE
Sbjct: 61  CNDEESLELVMKEISVLKSLKGHPNVVTLYAHGILDMGRNKKEALLAMDFCGKSLVDVLE 120

Query: 121 SRGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLLGSDGLWKLCDFGSTS 180
           +RGAGYF+EKQ L IFRDVCNAVF+MHC SP IAHRDLKAENLLL SDG WKLCDFGS S
Sbjct: 121 NRGAGYFEEKQALTIFRDVCNAVFAMHCQSPRIAHRDLKAENLLLSSDGQWKLCDFGSVS 180

Query: 181 TNHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELINEKVDIWALGCLLFRICYF 240
           TNHK FE+ EEMGIEEDNIRKYTTP YRAPEMWDLFRRE+I+EKVDIWALGCLLFRICYF
Sbjct: 181 TNHKIFERAEEMGIEEDNIRKYTTPTYRAPEMWDLFRREMISEKVDIWALGCLLFRICYF 240

Query: 241 KSAFDGESKLQILNGNYRIPELPKYSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLLPV 300
           K+AFDGESKLQILNGNYRIPE PKYS  ITDLI++MLQ SP++RPDITQ+WFRVN  LP 
Sbjct: 241 KNAFDGESKLQILNGNYRIPESPKYSVFITDLIKEMLQASPDERPDITQIWFRVNEQLPA 300

Query: 301 DSQKSLPDQPPDMPPTDKLEAGISNTMNKSPPMPRRSPPPPPSVKSSSQAAHVSKAAGAG 360
           + QKSLPD+PP+M  T  +  G S +  K  P PRRSPPPPP             ++G  
Sbjct: 301 NLQKSLPDRPPEMQSTG-VHDGSSKSATKPSPAPRRSPPPPP------------PSSGES 360

Query: 361 GGGGPMGAFWSTQHANDTTIKEDATRI-RFDEESTSRSTSKADKVNPNNHSTRKNASPGD 420
             GGP+GAFW+TQHA  + + ED   + +FDE ++  +TSK+++V  ++H  +K   P  
Sbjct: 361 DSGGPLGAFWATQHAKTSVVSEDNKNMPKFDEPNS--NTSKSERVRVDSHQPKK---PSP 420

Query: 421 INQKSNKTDSGSFRDFELSFFQNETECGSEESKGSKTESSAFQDKAFNNFVAEFGTANFS 480
           +  ++        +D E +  Q  T   +  +  + T  S  +D AFN+FVA+F T  F 
Sbjct: 421 VRGEARGIQRN--KDLETTISQKNT---TPAAANNMTRVS--KDDAFNSFVADFDTTKFD 480

Query: 481 SDVTNNRSGKEVALEAEVEKLKEQLKQANVEKTEITSKFEKLSAICRSQRQEIQELKQAL 540
           +    N+ GKE ALEAE+++LK++LKQ   EK EIT+KFEKLSAICRSQRQE+Q+LKQ L
Sbjct: 481 N---GNKPGKEEALEAEIQRLKDELKQTKSEKAEITAKFEKLSAICRSQRQELQDLKQTL 540

Query: 541 AARS-------------PSPNKVEMKNHNSRDIPPSTMPRLQ--KADVNAQSPDAKQWQA 600
           A++S             PSP    M +  SRD    TM  LQ  +++ +  S D   WQ 
Sbjct: 541 ASKSASPSPSRDSSQNQPSPGMHSMSSTPSRDKMEGTMWELQQDRSNWSTGSSDTNSWQP 600

Query: 601 FAEESPNQQPVNPERGVKSMRTRSLQRKQAAPEDTGFESWGFGAESF--TSVSAGSSNSS 660
           F++E+   +PV                + A+    GFE WGF  ESF   + SA ++++S
Sbjct: 601 FSDEA---KPV---------------MESASKGTQGFEPWGFETESFRAAATSAAATSAS 650

Query: 661 GL---AGEKSSSQQRIG--EPRNTDISSQPAGWAGF 673
           G     G  +S+ QR G  + R    ++QPAGWAGF
Sbjct: 661 GTQRSMGSGNSTSQRYGNSKMRENQKTAQPAGWAGF 650

BLAST of MS016130 vs. TAIR 10
Match: AT5G08160.1 (serine/threonine protein kinase 3 )

HSP 1 Score: 132.1 bits (331), Expect = 1.7e-30
Identity = 98/326 (30.06%), Postives = 149/326 (45.71%), Query Frame = 0

Query: 17  GRSIDVGNIKIHVRNVIAEGGFSCVYL--------------------AKDAVHLSKQ--Y 76
           G  + +   +  +   + EGGF+ V+L                     KD  HLS    Y
Sbjct: 18  GGDVWINENRFRIVRQLGEGGFAFVFLVKEIVADASSAASGGGLAKKVKDPAHLSADGTY 77

Query: 77  ALKHIICNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEA-------LLVM 136
           A+K ++  ++E LELV +E+ V  SL  HPN++ L  H II +   +E        LL  
Sbjct: 78  AMKKVLIQNKEQLELVREEIRV-SSLFNHPNLLPLLDHAIISVKDGQEGAWKHEAFLLFP 137

Query: 137 EFCEKSLVN--VLESRGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAHRDLKAENLLL- 196
              + +L++   L       F    VL IFR +C+ +  MH   PP AH D+K  N+LL 
Sbjct: 138 VHLDGTLLDNFTLMKAKKETFSTTDVLHIFRQLCDGLKHMHSLEPPYAHNDVKPGNVLLT 197

Query: 197 ---GSDGLWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKYTTPAYRAPEMWDLFRRELIN 256
              G   L  L DFGS   + K+    +E    ++   ++ +  +RAPE+WD      I+
Sbjct: 198 RRKGQPPLAILMDFGSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPELWDCPSHADID 257

Query: 257 EKVDIWALGCLLFRICYFKSAFD-------GESKLQILNGNYRIPEL-PK--YSPSITDL 298
           E+ DIW+LGC L+ I Y  S F+       G  +L I+N   + P   PK  Y  ++   
Sbjct: 258 ERTDIWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQIKWPNTGPKASYPEALHQF 317

BLAST of MS016130 vs. TAIR 10
Match: AT5G08160.2 (serine/threonine protein kinase 3 )

HSP 1 Score: 126.7 bits (317), Expect = 7.0e-29
Identity = 90/277 (32.49%), Postives = 134/277 (48.38%), Query Frame = 0

Query: 46  DAVHLSKQ--YALKHIICNDEESLELVLKEVSVMKSLRGHPNVVTLYAHTIIDMGRTKEA 105
           D  HLS    YA+K ++  ++E LELV +E+ V  SL  HPN++ L  H II +   +E 
Sbjct: 31  DPAHLSADGTYAMKKVLIQNKEQLELVREEIRV-SSLFNHPNLLPLLDHAIISVKDGQEG 90

Query: 106 -------LLVMEFCEKSLVN--VLESRGAGYFDEKQVLLIFRDVCNAVFSMHCHSPPIAH 165
                  LL     + +L++   L       F    VL IFR +C+ +  MH   PP AH
Sbjct: 91  AWKHEAFLLFPVHLDGTLLDNFTLMKAKKETFSTTDVLHIFRQLCDGLKHMHSLEPPYAH 150

Query: 166 RDLKAENLLL----GSDGLWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKYTTPAYRAPE 225
            D+K  N+LL    G   L  L DFGS   + K+    +E    ++   ++ +  +RAPE
Sbjct: 151 NDVKPGNVLLTRRKGQPPLAILMDFGSARPSRKQIRSRQEALQLQEWTSEHCSAPFRAPE 210

Query: 226 MWDLFRRELINEKVDIWALGCLLFRICYFKSAFD-------GESKLQILNGNYRIPEL-P 285
           +WD      I+E+ DIW+LGC L+ I Y  S F+       G  +L I+N   + P   P
Sbjct: 211 LWDCPSHADIDERTDIWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQIKWPNTGP 270

Query: 286 K--YSPSITDLIRDMLQGSPNDRPDITQVWFRVNGLL 298
           K  Y  ++   +  MLQ     RP I  +   V+ L+
Sbjct: 271 KASYPEALHQFVTWMLQPQAAVRPRIDDIIIHVDKLI 306

BLAST of MS016130 vs. TAIR 10
Match: AT1G03740.1 (Protein kinase superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 7.7e-20
Identity = 70/228 (30.70%), Postives = 116/228 (50.88%), Query Frame = 0

Query: 33  IAEGGFSCVYLAKDAVHLSKQYALKHI--ICNDEESLELVLKEVSVMKSLRGHPNVVTLY 92
           I +G +S VY A+D +H +K  ALK +    ND ES++ + +E+ VM+ L  HPNV+ L 
Sbjct: 219 IGQGTYSSVYRARDLLH-NKIVALKKVRFDLNDMESVKFMAREIIVMRRL-DHPNVLKL- 278

Query: 93  AHTIIDMGRTKEALLVMEFCEKSLVNVLESRGAGYFDEKQVLLIFRDVCNAVFSMHCHSP 152
              +I    +    LV E+ +  L+  L S     F E QV    R + + +   HCHS 
Sbjct: 279 -EGLITAPVSSSLYLVFEYMDHDLLG-LSSLPGVKFTEPQVKCYMRQLLSGL--EHCHSR 338

Query: 153 PIAHRDLKAENLLLGSDGLWKLCDFGSTSTNHKRFEKPEEMGIEEDNIRKYTTPAYRAPE 212
            + HRD+K  NLL+ S G+ K+ DFG  +     F+  + + +    +    T  YR PE
Sbjct: 339 GVLHRDIKGSNLLIDSKGVLKIADFGLATF----FDPAKSVSLTSHVV----TLWYRPPE 398

Query: 213 MWDLFRRELINEKVDIWALGCLLFRICYFKSAFDGESKLQILNGNYRI 259
           +  L         VD+W+ GC+L  +   K    G+++++ L+  +++
Sbjct: 399 L--LLGASHYGVGVDLWSTGCILGELYAGKPILPGKTEVEQLHKIFKL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152645.10.0e+0097.92probable serine/threonine-protein kinase DDB_G0276461 isoform X1 [Momordica char... [more]
XP_022152646.10.0e+0097.77probable serine/threonine-protein kinase DDB_G0276461 isoform X2 [Momordica char... [more]
XP_038904766.10.0e+0087.32probable serine/threonine-protein kinase DDB_G0276461 [Benincasa hispida] >XP_03... [more]
XP_004149621.10.0e+0086.87probable serine/threonine-protein kinase DDB_G0276461 [Cucumis sativus] >XP_0116... [more]
XP_008461768.10.0e+0086.24PREDICTED: probable serine/threonine-protein kinase DDB_G0276461 [Cucumis melo] ... [more]
Match NameE-valueIdentityDescription
F1SPM81.2e-5739.65AP2-associated protein kinase 1 OS=Sus scrofa OX=9823 GN=AAK1 PE=2 SV=2[more]
Q2M2I82.0e-5739.65AP2-associated protein kinase 1 OS=Homo sapiens OX=9606 GN=AAK1 PE=1 SV=3[more]
F1MH242.6e-5739.65AP2-associated protein kinase 1 OS=Bos taurus OX=9913 GN=AAK1 PE=1 SV=2[more]
Q3UHJ02.2e-5638.78AP2-associated protein kinase 1 OS=Mus musculus OX=10090 GN=Aak1 PE=1 SV=2[more]
O149765.5e-5539.64Cyclin-G-associated kinase OS=Homo sapiens OX=9606 GN=GAK PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DEI50.0e+0097.92probable serine/threonine-protein kinase DDB_G0276461 isoform X1 OS=Momordica ch... [more]
A0A6J1DID00.0e+0097.77probable serine/threonine-protein kinase DDB_G0276461 isoform X2 OS=Momordica ch... [more]
A0A0A0L9J90.0e+0086.87Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G702... [more]
A0A5A7TXP20.0e+0086.24Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CFB60.0e+0086.24probable serine/threonine-protein kinase DDB_G0276461 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT2G32850.21.2e-20156.45Protein kinase superfamily protein [more]
AT2G32850.13.8e-20056.47Protein kinase superfamily protein [more]
AT5G08160.11.7e-3030.06serine/threonine protein kinase 3 [more]
AT5G08160.27.0e-2932.49serine/threonine protein kinase 3 [more]
AT1G03740.17.7e-2030.70Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 489..537
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 11..304
e-value: 1.3E-81
score: 276.2
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 25..289
e-value: 1.5E-4
score: 18.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 625..666
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 555..598
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 537..672
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 378..397
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..430
NoneNo IPR availablePANTHERPTHR22967:SF57NUMB-ASSOCIATED KINASE, ISOFORM Acoord: 1..664
NoneNo IPR availablePANTHERPTHR22967SERINE/THREONINE PROTEIN KINASEcoord: 1..664
NoneNo IPR availableCDDcd13985STKc_GAK_likecoord: 26..298
e-value: 7.77694E-138
score: 402.868
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 27..291
e-value: 4.4E-43
score: 159.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..289
e-value: 2.3E-41
score: 141.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 27..297
score: 35.877052
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 152..164
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 28..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016130.1MS016130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity