MS015690 (gene) Bitter gourd (TR) v1

Overview
NameMS015690
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 6
Locationscaffold983: 296165 .. 300170 (+)
RNA-Seq ExpressionMS015690
SyntenyMS015690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCAGGATGATCTCGTCTGGCAAATTTTGGATTATTTTCTTTATATCGATTCCGAGCTTTTTACTCTCTTTTATCGAGCCGATTTCTTCTTCCGGCGGCGGTGATCACGGCGGCTCTGCTCTAATTGGCGCCGGATGCGGCGCCAAACCGATTCTGAATTCTGCCTCTCTGAAGAATCGGAGTATTGGAACGTTTATGTCTGGATTGGAATCTCAGAGCTGCGAGGTGTGGACGGACGCCTGCTCTGAGGCGATCCTCAGTTTGGCGAAGCGGACGGAGGTGGTTGATTGGTTGAAGAAGGTGAGGAGGAGGATCCACGAGAATCCGGAGCTTGCGTTTGAAGAATTCGAGACGAGTCAGTTGGTTCGCGATGAGTTGGACCGGATGGAAATTAGCTATCACCATCCTTTGGCTAAGACCGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTCGCTCTCAGAGCCGATATGGATGCTCTTCCAATCCAGGTTCTCCTTGGAAATCTGATATCTCCTTCATGATCATATGCTTCTGAATCTGCGATCGCTTTTTTTCTTTCCTCTTAATTCCGATCGATTGATTGCCTTGATGTCTCTGTTTAGCCTTGAAATTGGAAACAACGTGAGCGAGCGAATTAGAAAAGGAAGAAAACGCGGATTAGAATTTTAAAAAACCGCATCCTTAAAATTATTTTTGTAAATAAGTTAGCGAAATGATTCTTATCTGTTAAAATCCAGTTGTAATTTGCTCCTAAATATTTCATAGCTGAATTTCTACGAGCTCAGTTAGTTTTTCTTACTCCTTTCCTTACGAGTTGAAATTTCTTCTTTGTTCTTACTTTTAAGCTAATTTTTAGATTTTTATAAAAAAAATATCTCATCGAATATTTTTTTAGTCAATTTTCATTTACAATGGAGATATAGATTGTAAAAACTATTCTTCATTCTTGAAGAAGTCGTAGAGTTTTTCTTTGTTATTATTATAATAGCGTGAGTGACTCAGGGCATAATAGTGGTACTGTATCTTTTGGATCTCTCTCTCTCTCTCAATTTTTTTGCACGTGTTTAAATCTCTTCTTTAAGATAAATAAAATAATATAACTTATTGAGAAAGAATAGTTGGATTAAATGACATGGTTTAGAAATATGGTATTTTATTATTATTATTTTTTTTTTGAAAGTCAGTACAATCCATAGCCCCATAAATTTTCCAGATAAAAGGATAAGTGGAAATGGTAGACATGGTTTCTGTTGTACCTTCGAACAGGTTGAGGTTGAGGTTGAGGTTGAGGTGGGTCATAACATTTACGAAATTTTCTAGGTATATTGGATATTTTAGTCCGTATTTAAGTTCTACACCATAAATTACGGATGGTTGAACTGAATCTGATCAATCTCCTTTTTTGTCCATAAATTTTAATATTTATTTTTAATTTTTGAACTTTTTTTAAGGAAAACTATTCTTTAAACTTTGTTTTGTTTTAGCCTTTAAATCTTACCTAACTAATTATTTCAGTCCTTAACAAAACTTCCCTTAAGATCTTCACATCTAAACATGTGCATTATTAATTAGGTAAGACGAACGAAATACATGTTACATAATGATATATGAGAAATGACAAAGAAGTAAAAGGATTTGACTTCACAACTTAAAAAATACTAAAATAAAATGCCTGTAATTTTAAGACAAATGCTATGGAATGGGGATTCTATCTGTTTTAGGACTTGAGTTGTTACAATGATTAATTTCTTGACACTATTTTTAATGAGATTAGAAAATGTGGAAAGGCATTGAAGAATGAGCTTTAAAATTAATGGTGCAGGAGGCTGTGGAATGGGAACACAAAAGCAGAGTCCAAGGCAAGATGCATGCTTGTGGGCACGATGCTCATGTTACAATGCTTCTTGGTGCTGCCAAGATCTTAAAGGATCGTGAACATCTATTGAAGGTTTGTCAAACGCCTCTCATTTTTCGATTTGCTTGAGAAAAGAAACAACGAAAATTTAGTTTTTAGAAAATTAATATGATAATATTATTTCATCTTTAAGAATTTGCAGTAGTTAAAAATTCACTGATGTTTAGTGGGTACTTTCTGTTCTTTGATTTCAAAGCCTCGAAGTTGATTCAATTTTTTCAGGGAAATGAGGCATCAAAATTATCGAATTTAAGGAAACATTATTCTGCCAAAATTGTGGGCAAATTCTTTTGCAAAAAGGAAGGGGGGATGGGAAAGTTGACTTTTTCAGTTGCAGAAATTGAAAATATAGACTACGAATAGTCACTAACCATAATAAATACATAGGAAGTCCTTTTAACTGTAAATGAGAAGAAATTAATGGCTCTTGAAGTCTTCTCGTTGCAGGTACAATGCATAAACTACTTTATTCTAGCTTTAGTTCTATATGGACAATTGGCCTTTTTTTTCATTACCAAAACAGTTCTCAACTTCCCCAATTAAGTGGCTGTAAAAGTTATAATTATTTTATCTTTAAATCACTATTTACAGGACCATAATTTAAATGGAAGAAGTCATAGGTAGAAACAAACAAAAAGTCCAAAAGAAAAAACAGAACATTGCTTTCTGAAAACGGCTCCCAAACAACTACTACTGAGTAGCTTATTACTCTTCTGTTGTCTTTTCTCACGTGTTCTTCTTTTCTGCTATCTTTTCCCTCTTAATTCCGATCATTAATCTATACCACTTTGCAAACNAAACAATCAACAGGGAAGTTTTGTTCTTTTTTACTCGGAATTTAGCATCTGCACATTTTTTTTCCCAAAAGGTTATCCCATTCCATGAACACAGTCGATTTTTTTTTCCAATAAGTAGACCAGCAAGTAACATGATTTCTCAGATTCTCTGTCGGCATAATGTTGTCTCGAGTTGTATCTTATCTTTATTCTTTTGGCTGTGGGGTACTAAAAAATCTGGAATCTGTGATCGCAGTTCACTTACCATTGTACTGTTTTACAGGGGACGGTGATTCTGCTGTTTCAGCCAGCCGAGGAAGCCGGCAATGGCGCAAAGCGGATGATCGGCGATGGGGCGCTGAAAGACGTGCAGGCCATTTTTGCAGTTCATGTTTCTCACGAGCATCCCACCGGCATCATTGGGTCGCGCCCCGGCCCTCTTCTCGCCGGCTGCGGCTTCTTTCGGGCGGTGATTACCGGCAAGAAGGGCCGCGCCGGATCGCCCCACCACTCCGTCGACCCGGTCCTGGCCGCCGCCGCCGCCGTTATCAGCCTGCAGGGGATCGTCTCTCGAGAGGCAAACCCTTTGGATTCGCAGGTTTTGAAACCACCTCCTCTCATCAGTTTCTTCGGAAATATAATATTGTTGGTAATTATTTTGCTGCCAATGAGTTTTCTTTAGGGAATAATTGATTAATTAATTGCGTCTTAATTAGGTTGTCTCTGTAACTTCGTTCAATGGAGGCAATAATCTGGATATGATACCGGATACCGTGGTTATTGGCGGTACCTTCAGGGCTTTCTCCAATTCAAGCTTCTACCAAGTTCTTCGCAGAATACAGGAGGTAATTAAGCAATTAATTAGGATTTATTTTAAGAAGTCATAAAAAAAAACTCGACTTTAATTCTAAAGTAAATTTTGATTGTACCGTATAGGTTATCGTGGAGCAGGCGAGCGTTTACAGATGCTCGGGCACAGTGGACTTCTTTGAAAAGGAATACACCATATACCCTCCAACTGTAAACGACGAAAAGATGTACGAGCATGTGAAAAAGGTGGCCATTGATTTGTTGGGTTCGCACAATTTCAAGGTGGTGCCGCCAATGATGGGGGCCGAGGACTTCTCCTTCTACACAGAGTTTGTTCCCGCAGCCTTTTTCTACATAGGAGTCAGAAACGAGACTCTAGGATCCATCCACACCGGCCACTCCCCCTACTTCATGATCGACGAAGATGTTCTACCGATCGGTGCTGCTACTCACGCAACCATTGCTGAGAGGTACCTATACGAGCATGGCGAA

mRNA sequence

TTCTTCAGGATGATCTCGTCTGGCAAATTTTGGATTATTTTCTTTATATCGATTCCGAGCTTTTTACTCTCTTTTATCGAGCCGATTTCTTCTTCCGGCGGCGGTGATCACGGCGGCTCTGCTCTAATTGGCGCCGGATGCGGCGCCAAACCGATTCTGAATTCTGCCTCTCTGAAGAATCGGAGTATTGGAACGTTTATGTCTGGATTGGAATCTCAGAGCTGCGAGGTGTGGACGGACGCCTGCTCTGAGGCGATCCTCAGTTTGGCGAAGCGGACGGAGGTGGTTGATTGGTTGAAGAAGGTGAGGAGGAGGATCCACGAGAATCCGGAGCTTGCGTTTGAAGAATTCGAGACGAGTCAGTTGGTTCGCGATGAGTTGGACCGGATGGAAATTAGCTATCACCATCCTTTGGCTAAGACCGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTCGCTCTCAGAGCCGATATGGATGCTCTTCCAATCCAGGAGGCTGTGGAATGGGAACACAAAAGCAGAGTCCAAGGCAAGATGCATGCTTGTGGGCACGATGCTCATGTTACAATGCTTCTTGGTGCTGCCAAGATCTTAAAGGATCGTGAACATCTATTGAAGGGGACGGTGATTCTGCTGTTTCAGCCAGCCGAGGAAGCCGGCAATGGCGCAAAGCGGATGATCGGCGATGGGGCGCTGAAAGACGTGCAGGCCATTTTTGCAGTTCATGTTTCTCACGAGCATCCCACCGGCATCATTGGGTCGCGCCCCGGCCCTCTTCTCGCCGGCTGCGGCTTCTTTCGGGCGGTGATTACCGGCAAGAAGGGCCGCGCCGGATCGCCCCACCACTCCGTCGACCCGGTCCTGGCCGCCGCCGCCGCCGTTATCAGCCTGCAGGGGATCGTCTCTCGAGAGGCAAACCCTTTGGATTCGCAGGTTGTCTCTGTAACTTCGTTCAATGGAGGCAATAATCTGGATATGATACCGGATACCGTGGTTATTGGCGGTACCTTCAGGGCTTTCTCCAATTCAAGCTTCTACCAAGTTCTTCGCAGAATACAGGAGGTTATCGTGGAGCAGGCGAGCGTTTACAGATGCTCGGGCACAGTGGACTTCTTTGAAAAGGAATACACCATATACCCTCCAACTGTAAACGACGAAAAGATGTACGAGCATGTGAAAAAGGTGGCCATTGATTTGTTGGGTTCGCACAATTTCAAGGTGGTGCCGCCAATGATGGGGGCCGAGGACTTCTCCTTCTACACAGAGTTTGTTCCCGCAGCCTTTTTCTACATAGGAGTCAGAAACGAGACTCTAGGATCCATCCACACCGGCCACTCCCCCTACTTCATGATCGACGAAGATGTTCTACCGATCGGTGCTGCTACTCACGCAACCATTGCTGAGAGGTACCTATACGAGCATGGCGAA

Coding sequence (CDS)

TTCTTCAGGATGATCTCGTCTGGCAAATTTTGGATTATTTTCTTTATATCGATTCCGAGCTTTTTACTCTCTTTTATCGAGCCGATTTCTTCTTCCGGCGGCGGTGATCACGGCGGCTCTGCTCTAATTGGCGCCGGATGCGGCGCCAAACCGATTCTGAATTCTGCCTCTCTGAAGAATCGGAGTATTGGAACGTTTATGTCTGGATTGGAATCTCAGAGCTGCGAGGTGTGGACGGACGCCTGCTCTGAGGCGATCCTCAGTTTGGCGAAGCGGACGGAGGTGGTTGATTGGTTGAAGAAGGTGAGGAGGAGGATCCACGAGAATCCGGAGCTTGCGTTTGAAGAATTCGAGACGAGTCAGTTGGTTCGCGATGAGTTGGACCGGATGGAAATTAGCTATCACCATCCTTTGGCTAAGACCGGCGTTCGTGCTTGGATCGGGACTGGCGGACCGCCTTTTGTCGCTCTCAGAGCCGATATGGATGCTCTTCCAATCCAGGAGGCTGTGGAATGGGAACACAAAAGCAGAGTCCAAGGCAAGATGCATGCTTGTGGGCACGATGCTCATGTTACAATGCTTCTTGGTGCTGCCAAGATCTTAAAGGATCGTGAACATCTATTGAAGGGGACGGTGATTCTGCTGTTTCAGCCAGCCGAGGAAGCCGGCAATGGCGCAAAGCGGATGATCGGCGATGGGGCGCTGAAAGACGTGCAGGCCATTTTTGCAGTTCATGTTTCTCACGAGCATCCCACCGGCATCATTGGGTCGCGCCCCGGCCCTCTTCTCGCCGGCTGCGGCTTCTTTCGGGCGGTGATTACCGGCAAGAAGGGCCGCGCCGGATCGCCCCACCACTCCGTCGACCCGGTCCTGGCCGCCGCCGCCGCCGTTATCAGCCTGCAGGGGATCGTCTCTCGAGAGGCAAACCCTTTGGATTCGCAGGTTGTCTCTGTAACTTCGTTCAATGGAGGCAATAATCTGGATATGATACCGGATACCGTGGTTATTGGCGGTACCTTCAGGGCTTTCTCCAATTCAAGCTTCTACCAAGTTCTTCGCAGAATACAGGAGGTTATCGTGGAGCAGGCGAGCGTTTACAGATGCTCGGGCACAGTGGACTTCTTTGAAAAGGAATACACCATATACCCTCCAACTGTAAACGACGAAAAGATGTACGAGCATGTGAAAAAGGTGGCCATTGATTTGTTGGGTTCGCACAATTTCAAGGTGGTGCCGCCAATGATGGGGGCCGAGGACTTCTCCTTCTACACAGAGTTTGTTCCCGCAGCCTTTTTCTACATAGGAGTCAGAAACGAGACTCTAGGATCCATCCACACCGGCCACTCCCCCTACTTCATGATCGACGAAGATGTTCTACCGATCGGTGCTGCTACTCACGCAACCATTGCTGAGAGGTACCTATACGAGCATGGCGAA

Protein sequence

FFRMISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSIGTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Homology
BLAST of MS015690 vs. NCBI nr
Match: XP_038885213.1 (IAA-amino acid hydrolase ILR1-like 6 [Benincasa hispida])

HSP 1 Score: 848.2 bits (2190), Expect = 3.5e-242
Identity = 423/476 (88.87%), Postives = 446/476 (93.70%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           MISS  F+IIFFI I +F LS   P+    GGDHGGS LIGAGCG +P+  S SLKN+SI
Sbjct: 1   MISSANFFIIFFIVIHTFSLSSSSPV----GGDHGGSPLIGAGCGLQPLRVSNSLKNQSI 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           G F   LESQSCEVWT+ACSEAIL+LAKRTEVVDWLKKVRRRIHENPELAFEEFETSQL+
Sbjct: 61  GAFR--LESQSCEVWTEACSEAILTLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLI 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ISY HPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDISYEHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILK+REHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKEREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHEHPT +IGSRPGPLLAGCGFFRAVITGKKGRAGSPH SVDPVLAA+AAV+SLQGI
Sbjct: 241 VHVSHEHPTAVIGSRPGPLLAGCGFFRAVITGKKGRAGSPHRSVDPVLAASAAVVSLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLQRIEEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFEKEYTIYPPTVNDE+MYEHVKKVAIDL GS NF++V PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEKEYTIYPPTVNDERMYEHVKKVAIDLHGSQNFRLVQPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           +EFVPAAF+YIGVRNETLGSIHTGHSPYFMIDE+VLPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPAAFYYIGVRNETLGSIHTGHSPYFMIDENVLPIGAATHATIAERYLYEHGE 470

BLAST of MS015690 vs. NCBI nr
Match: KAG6578962.1 (IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 838.2 bits (2164), Expect = 3.6e-239
Identity = 414/476 (86.97%), Postives = 444/476 (93.28%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M +S +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFASARPCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGLQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ISY +PLAKTG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDISYEYPLAKTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVTSFNGGNNLD+IPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNNLDIIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NFKVV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLHGSPNFKVVQPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. NCBI nr
Match: XP_023551590.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 836.6 bits (2160), Expect = 1.0e-238
Identity = 412/476 (86.55%), Postives = 443/476 (93.07%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M SS +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFSSARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGLQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETS+LV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSRLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ISY +PLAKTG+R WIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDISYEYPLAKTGIRGWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVTSFNGGNNLD+IPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNNLDIIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NF+VV PMMGAEDFSFY
Sbjct: 361 SVYRCSAIVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLHGSPNFRVVQPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. NCBI nr
Match: XP_022939072.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita moschata])

HSP 1 Score: 834.7 bits (2155), Expect = 4.0e-238
Identity = 412/476 (86.55%), Postives = 443/476 (93.07%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M SS +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFSSARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGFQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           R ELDRM+ISY +PLAKTG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RGELDRMDISYEYPLAKTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVTSFNGGN+LD+IPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNSLDIIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NF+VV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLHGSPNFRVVQPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. NCBI nr
Match: XP_022993486.1 (IAA-amino acid hydrolase ILR1-like 6 [Cucurbita maxima])

HSP 1 Score: 831.2 bits (2146), Expect = 4.4e-237
Identity = 410/476 (86.13%), Postives = 441/476 (92.65%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M S  +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFSPARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGLQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ISY +PLA+TG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDISYEYPLAQTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVT FNGG +LDMIPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGKDLDMIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NF+VV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLYGSPNFRVVRPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. ExPASy Swiss-Prot
Match: Q8VYX0 (IAA-amino acid hydrolase ILR1-like 6 OS=Arabidopsis thaliana OX=3702 GN=ILL6 PE=1 SV=2)

HSP 1 Score: 607.1 bits (1564), Expect = 1.8e-172
Identity = 294/423 (69.50%), Postives = 350/423 (82.74%), Query Frame = 0

Query: 52  ILNSASLKNRSIGTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPE 111
           +L    +KN+S+G   + + S  C VWT ACS+ IL L  + + V WLK+VRR IHENPE
Sbjct: 43  LLRPTPIKNQSLG-LPAHVGSDECRVWTKACSDEILRLTYQPDNVAWLKRVRRTIHENPE 102

Query: 112 LAFEEFETSQLVRDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVE 171
           LAFEE+ETS+L+R ELDRM I Y +PLAKTG+RAWIG+GGPPFVA+RADMDALPIQEAVE
Sbjct: 103 LAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAWIGSGGPPFVAVRADMDALPIQEAVE 162

Query: 172 WEHKSRVQGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIG 231
           WEH S+V GKMHACGHDAHVTMLLGAA ILK REHLLKGTV+LLFQPAEEAGNGAK MI 
Sbjct: 163 WEHISKVAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTVVLLFQPAEEAGNGAKNMIE 222

Query: 232 DGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVL 291
           DGAL DV+AIFAVHVSH HPTG+IGSR GPLLAGCG FRAVIT +  R      + + +L
Sbjct: 223 DGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAVITSEDSRG-----AANLLL 282

Query: 292 AAAAAVISLQGIVSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQV 351
           AA++AVISLQGIVSREA+PLDSQVVSVTSF+GG++LD+ PDTVV+GGTFRAFSNSSFY +
Sbjct: 283 AASSAVISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYL 342

Query: 352 LRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVV 411
            +RIQEV+++Q  V+ C  TV+FFEK+  IYPPT N++  Y H+KKV IDLLG  +F + 
Sbjct: 343 KKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLA 402

Query: 412 PPMMGAEDFSFYTEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAE 471
           P MMGAEDF+FY+E +PAAF++IG+RNE LGS+H  HSP+FMIDED LP+GAA HA +AE
Sbjct: 403 PQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAE 459

Query: 472 RYL 475
           RYL
Sbjct: 463 RYL 459

BLAST of MS015690 vs. ExPASy Swiss-Prot
Match: Q5Z678 (IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL6 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 4.5e-152
Identity = 278/402 (69.15%), Postives = 316/402 (78.61%), Query Frame = 0

Query: 84  EAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGV 143
           E I  +A R E   WL+ VRRRIHE PELA+EE ETS+LVRDELD M + + HP+A+TGV
Sbjct: 97  EEIAGMAGRPETAAWLRAVRRRIHERPELAYEEVETSRLVRDELDAMGVGFRHPVARTGV 156

Query: 144 RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKD 203
            A IGTG PP VALRADMDALPIQEAVEWEHKS+  GKMHACGHDAHV MLLGAAKILK 
Sbjct: 157 VANIGTGRPPVVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDAHVAMLLGAAKILKA 216

Query: 204 REHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLL 263
           REH L+GTV LLFQPAEE+G GAKRMI  GAL+DV+AIFAVHVSH+HPT +IGSR GPLL
Sbjct: 217 REHHLRGTVRLLFQPAEESGAGAKRMIEGGALEDVEAIFAVHVSHQHPTSVIGSRTGPLL 276

Query: 264 AGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNG 323
           AGCGFF+AVI G  GR      S D VLAAA+ +ISLQ IVSREA+PLDSQVVSV   NG
Sbjct: 277 AGCGFFKAVIHG--GR-----RSGDAVLAAASTIISLQSIVSREADPLDSQVVSVAMVNG 336

Query: 324 GNNLDMI--------PDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFF 383
            ++             +  V+GGTFRAFSN+SFYQV RRI+EVI  QA V+ C   VDFF
Sbjct: 337 SDHPAATARAAAAEEEEEFVLGGTFRAFSNASFYQVRRRIEEVITAQARVHGCEAAVDFF 396

Query: 384 EKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFYIG 443
           E + + YPPTVND +MY HVK VA +LLG+ +++ VPPMMGAEDFSFY++ VPA F+YIG
Sbjct: 397 ENQ-SFYPPTVNDARMYAHVKAVAGELLGAGSYRDVPPMMGAEDFSFYSQVVPAGFYYIG 456

Query: 444 VRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEH 478
           VRNETLGS+HTGHSPYFMIDEDVLP GAA HA IAERYL  H
Sbjct: 457 VRNETLGSVHTGHSPYFMIDEDVLPTGAAFHAAIAERYLANH 490

BLAST of MS015690 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.5e-136
Identity = 231/393 (58.78%), Postives = 297/393 (75.57%), Query Frame = 0

Query: 86  ILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRA 145
           +L  AK  E   W+  +RRRIHENPEL +EEF TS+LVR ELD + I Y HP A TGV A
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 146 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDRE 205
            +GTGGPPFVALRADMDALP+QE+VEWEHKS+V GKMH CGHDAHV MLLG+A+IL++  
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 206 HLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAG 265
             LKGTV+L+FQPAEE G GAK+MI DGA+++++AIF VHV+   P G++ SRPGP++AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 266 CGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGN 325
            GFF AVI+GK G A  PHH++DP+LAA+  ++SLQ +VSREA+PLDSQVV+V  F GG 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 326 NLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPT 385
             ++IPD+V IGGTFRAF   SF Q+ +RI+EVIV QASV RC+  VDF +K+   +PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 386 VNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFY-IGVRNETLGSI 445
           +N   +++   KVA +++G  N +   P+MGAEDF+FY + +PA ++Y +G+ NET G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 446 HTGHSPYFMIDEDVLPIGAATHATIAERYLYEH 478
              HSPYF I+ED LP GAA  A++A RYL EH
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLAARYLLEH 428

BLAST of MS015690 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.0e-135
Identity = 230/393 (58.52%), Postives = 296/393 (75.32%), Query Frame = 0

Query: 86  ILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRA 145
           +L  AK  E   W+  +RRRIHENPEL +EEF TS+LVR ELD + I Y HP A TGV A
Sbjct: 36  LLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVA 95

Query: 146 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDRE 205
            +GTGGPPFVALRADMDALP+QE+VEWEHKS+V GKMH CGHDAHV MLLG+A+IL++  
Sbjct: 96  TVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHR 155

Query: 206 HLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAG 265
             LKGTV+L+FQPAEE G GAK+MI DG +++++AIF VHV+   P G++ SRPGP++AG
Sbjct: 156 DELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAG 215

Query: 266 CGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGN 325
            GFF AVI+GK G A  PHH++DP+LAA+  ++SLQ +VSREA+PLDSQVV+V  F GG 
Sbjct: 216 SGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGG 275

Query: 326 NLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPT 385
             ++IPD+V IGGTFRAF   SF Q+ +RI+EVIV QASV RC+  VDF +K+   +PPT
Sbjct: 276 AFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPT 335

Query: 386 VNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFY-IGVRNETLGSI 445
           +N   +++   KVA +++G  N +   P+MGAEDF+FY + +PA ++Y +G+ NET G  
Sbjct: 336 INSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQ 395

Query: 446 HTGHSPYFMIDEDVLPIGAATHATIAERYLYEH 478
              HSPYF I+ED LP GAA  A++A RYL EH
Sbjct: 396 APHHSPYFTINEDALPYGAALQASLATRYLLEH 428

BLAST of MS015690 vs. ExPASy Swiss-Prot
Match: Q5N8F2 (IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL2 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 7.2e-134
Identity = 232/394 (58.88%), Postives = 298/394 (75.63%), Query Frame = 0

Query: 86  ILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRA 145
           +L  A+R E   W+  VR  IHE PELAFEE ETS+LVR ELD M ++Y HP+A TGV A
Sbjct: 50  VLDRARRPEFAAWMAGVRWAIHERPELAFEEIETSRLVRAELDAMGVAYRHPVAGTGVVA 109

Query: 146 WIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDRE 205
            +GTG PPFVALRADMDALP+QE V+WEHKS+V  KMHACGHDAH TMLLGAA+IL++R 
Sbjct: 110 TVGTGRPPFVALRADMDALPMQEEVQWEHKSKVAMKMHACGHDAHTTMLLGAARILQERR 169

Query: 206 HLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAG 265
           H L+GTV+LLFQP EE G GA+RM+  GA+ +V+AIF  HVS E PTG++GSRPGPLLAG
Sbjct: 170 HELQGTVVLLFQPGEEVGTGARRMVEAGAVDNVEAIFGFHVSVELPTGVVGSRPGPLLAG 229

Query: 266 CGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGN 325
           CGFF AVITGK G A  PH SVDP+LAA+  V++LQG+VSREA+PL++QVV+VT F  G+
Sbjct: 230 CGFFEAVITGKGGHAAHPHASVDPILAASTVVLALQGLVSREADPLEAQVVTVTRFLAGD 289

Query: 326 NLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPT 385
            L++IP+++ IGGTFR FSN  F ++ RRI+EVIV Q++VYRC+  VDF      + PPT
Sbjct: 290 ALNVIPESITIGGTFRVFSNEGFLRLKRRIEEVIVAQSAVYRCAAAVDFHAGGRPLLPPT 349

Query: 386 VNDEKMYEHVKKVAIDLLGSHN--FKVVPPMMGAEDFSFYTEFVPAA-FFYIGVRNETLG 445
           +N   ++ H + VA + LG+       + P MG+EDF+ ++E VPA+ F+++GVRNE  G
Sbjct: 350 INSAALHAHFQAVAAETLGASAAVLGAMEPCMGSEDFAVFSEAVPASHFYFVGVRNEAEG 409

Query: 446 SIHTGHSPYFMIDEDVLPIGAATHATIAERYLYE 477
            +H  HSP+F +D+  LP GAA HA++A RYL E
Sbjct: 410 LVHLAHSPHFRVDDAALPYGAALHASLAMRYLDE 443

BLAST of MS015690 vs. ExPASy TrEMBL
Match: A0A6J1FFS9 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC111445084 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.9e-238
Identity = 412/476 (86.55%), Postives = 443/476 (93.07%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M SS +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFSSARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGFQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           R ELDRM+ISY +PLAKTG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RGELDRMDISYEYPLAKTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVTSFNGGN+LD+IPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTSFNGGNSLDIIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NF+VV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLHGSPNFRVVQPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. ExPASy TrEMBL
Match: A0A6J1JWG3 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111489479 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.1e-237
Identity = 410/476 (86.13%), Postives = 441/476 (92.65%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M S  +  +IFFI+I SF +  I+ I S  GGDHGGS+LIGAGCG + + NS SLKN+S+
Sbjct: 1   MFSPARLCLIFFIAIQSFSVCLIDAIFSPVGGDHGGSSLIGAGCGLQTVRNSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
           GT M+ LESQ+CEVWT+ACSEAILSLAKR EVVDWLKKVRR IHENPELAFEEFETSQLV
Sbjct: 61  GTLMTRLESQNCEVWTEACSEAILSLAKRPEVVDWLKKVRRIIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ISY +PLA+TG+RAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDISYEYPLAQTGIRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE+PT  IGSRPGPLLAGCGFF+AVITGK GRAGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHEYPTATIGSRPGPLLAGCGFFKAVITGKMGRAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVT FNGG +LDMIPDTVVIGGTFRA+SNSSFYQVL+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGKDLDMIPDTVVIGGTFRAYSNSSFYQVLQRIKEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
           SVYRCS  VDFFE+EYTIYPPTVNDEKMYEHVKKVAIDL GS NF+VV PMMGAEDFSFY
Sbjct: 361 SVYRCSAMVDFFEREYTIYPPTVNDEKMYEHVKKVAIDLYGSPNFRVVRPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           T+ VPAAF+Y+G+RNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE
Sbjct: 421 TQLVPAAFYYVGIRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. ExPASy TrEMBL
Match: A0A6J1GUP6 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC111457704 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.8e-236
Identity = 412/476 (86.55%), Postives = 438/476 (92.02%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M SS  F IIF I I  F +SFI+ ISS  GGDH GSALIGAG G   +  S SLKN+S+
Sbjct: 1   MDSSLNFCIIFTIVIQCFSISFIDAISSPVGGDHDGSALIGAGSGINSVPESNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
            T M+  +SQSCEVWT+ACSEAILSLAKR +VVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ SY HP AKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAAKILKDREHLLKGTV+LLFQPAEEAGNGAKRMIGDGAL++VQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAKILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE PT IIGSRPGPLLAGCGFFRAVITGKKG+AGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVT FNGG+NLD+IPDTVVIGGTFRAFSNSSFYQ+L+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSNLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
            VYRCS TVDFFEKEYTIYPPTVNDEKMYEHVKKVA DL GSHN++VVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTVNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           +EFVP A++YIGVRNETLGSIHTGHSPYFMIDED+LPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 476

BLAST of MS015690 vs. ExPASy TrEMBL
Match: A0A0A0KDD2 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G384060 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.3e-234
Identity = 413/479 (86.22%), Postives = 435/479 (90.81%), Query Frame = 0

Query: 1   FFRMISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKN 60
           F  MISS  F+ IF I I  F LS     SS  GGDHGGS L+GA C  +P+  S SLKN
Sbjct: 84  FLTMISSLNFFSIFIIFIHFFSLS----SSSPAGGDHGGSPLVGAACALQPLRISNSLKN 143

Query: 61  RSIGTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETS 120
           +SIG  +S L SQSCEVWT+ACSEAILSLAKR EVVDWLKKVRRRIHENPELAFEEFETS
Sbjct: 144 QSIGALVSQLASQSCEVWTEACSEAILSLAKRPEVVDWLKKVRRRIHENPELAFEEFETS 203

Query: 121 QLVRDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQG 180
           QL+RDELDRMEISY H LAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV G
Sbjct: 204 QLIRDELDRMEISYEHMLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVAG 263

Query: 181 KMHACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQA 240
           KMHACGHDAHVTMLLGAAKILK REHLLKGTVILLFQPAEEAGNGAKRMIGDGAL+DVQA
Sbjct: 264 KMHACGHDAHVTMLLGAAKILKAREHLLKGTVILLFQPAEEAGNGAKRMIGDGALRDVQA 323

Query: 241 IFAVHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISL 300
           IFA HVSHEHPT +IGSRPGPLLAGCGFFRAVITGKKG AGSPH SVDPVLAA+AAV+SL
Sbjct: 324 IFAAHVSHEHPTAVIGSRPGPLLAGCGFFRAVITGKKGHAGSPHRSVDPVLAASAAVVSL 383

Query: 301 QGIVSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIV 360
           QGIVSREANPLDSQVVSVTSFNGG+NLDMIPD VVIGGTFRAFSNSSFYQVL+RI++VIV
Sbjct: 384 QGIVSREANPLDSQVVSVTSFNGGSNLDMIPDVVVIGGTFRAFSNSSFYQVLQRIEQVIV 443

Query: 361 EQASVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDF 420
           EQASVYRCS  VDFFEKEYTIYPPTVND+ MYEHVKKVAIDL GS NF++V PMMGAEDF
Sbjct: 444 EQASVYRCSAMVDFFEKEYTIYPPTVNDKAMYEHVKKVAIDLHGSQNFRIVQPMMGAEDF 503

Query: 421 SFYTEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           SFY+E+VPAAFFYIGVRNETLGSIHTGHSPYFMIDE+VLPIGAATHATIAERYLYEHGE
Sbjct: 504 SFYSEYVPAAFFYIGVRNETLGSIHTGHSPYFMIDENVLPIGAATHATIAERYLYEHGE 558

BLAST of MS015690 vs. ExPASy TrEMBL
Match: A0A6J1JS37 (IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111487050 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.5e-232
Identity = 407/476 (85.50%), Postives = 436/476 (91.60%), Query Frame = 0

Query: 4   MISSGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSI 63
           M SS  F IIF I +  F +SFI+ ISS  GGDH GSALIGA  G   + +S SLKN+S+
Sbjct: 1   MDSSLNFCIIFTI-VQCFSISFIDTISSPVGGDHDGSALIGADSGINSVPDSNSLKNQSV 60

Query: 64  GTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLV 123
            T M+  +SQSCEVWT+ACSEAILSLAKR +VVDWLKKVRRRIHENPELAFEEFETSQLV
Sbjct: 61  RTLMARSDSQSCEVWTEACSEAILSLAKRPDVVDWLKKVRRRIHENPELAFEEFETSQLV 120

Query: 124 RDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMH 183
           RDELDRM+ SY HP AKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRV GKMH
Sbjct: 121 RDELDRMDASYEHPFAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVPGKMH 180

Query: 184 ACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFA 243
           ACGHDAHVTMLLGAA ILKDREHLLKGTV+LLFQPAEEAGNGAKRMIGDGAL++VQAIFA
Sbjct: 181 ACGHDAHVTMLLGAAMILKDREHLLKGTVVLLFQPAEEAGNGAKRMIGDGALRNVQAIFA 240

Query: 244 VHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGI 303
           VHVSHE PT IIGSRPGPLLAGCGFFRAVITGKKG+AGSPH SVDPVLAA+AAVISLQGI
Sbjct: 241 VHVSHELPTAIIGSRPGPLLAGCGFFRAVITGKKGQAGSPHRSVDPVLAASAAVISLQGI 300

Query: 304 VSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQA 363
           VSREANPLDSQVVSVT FNGG+ LD+IPDTVVIGGTFRAFSNSSFYQ+L+RI+EVIVEQA
Sbjct: 301 VSREANPLDSQVVSVTWFNGGSKLDVIPDTVVIGGTFRAFSNSSFYQLLQRIEEVIVEQA 360

Query: 364 SVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFY 423
            VYRCS TVDFFEKEYTIYPPT+NDEKMYEHVKKVA DL GSHN++VVPPMMGAEDFSFY
Sbjct: 361 RVYRCSTTVDFFEKEYTIYPPTMNDEKMYEHVKKVATDLHGSHNYRVVPPMMGAEDFSFY 420

Query: 424 TEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHGE 480
           +EFVP A++YIGVRNETLGSIHTGHSPYFMIDED+LPIGAATHATIAERYLYEHGE
Sbjct: 421 SEFVPTAYYYIGVRNETLGSIHTGHSPYFMIDEDMLPIGAATHATIAERYLYEHGE 475

BLAST of MS015690 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 607.1 bits (1564), Expect = 1.3e-173
Identity = 294/423 (69.50%), Postives = 350/423 (82.74%), Query Frame = 0

Query: 52  ILNSASLKNRSIGTFMSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPE 111
           +L    +KN+S+G   + + S  C VWT ACS+ IL L  + + V WLK+VRR IHENPE
Sbjct: 43  LLRPTPIKNQSLG-LPAHVGSDECRVWTKACSDEILRLTYQPDNVAWLKRVRRTIHENPE 102

Query: 112 LAFEEFETSQLVRDELDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVE 171
           LAFEE+ETS+L+R ELDRM I Y +PLAKTG+RAWIG+GGPPFVA+RADMDALPIQEAVE
Sbjct: 103 LAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAWIGSGGPPFVAVRADMDALPIQEAVE 162

Query: 172 WEHKSRVQGKMHACGHDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIG 231
           WEH S+V GKMHACGHDAHVTMLLGAA ILK REHLLKGTV+LLFQPAEEAGNGAK MI 
Sbjct: 163 WEHISKVAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTVVLLFQPAEEAGNGAKNMIE 222

Query: 232 DGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVL 291
           DGAL DV+AIFAVHVSH HPTG+IGSR GPLLAGCG FRAVIT +  R      + + +L
Sbjct: 223 DGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAVITSEDSRG-----AANLLL 282

Query: 292 AAAAAVISLQGIVSREANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQV 351
           AA++AVISLQGIVSREA+PLDSQVVSVTSF+GG++LD+ PDTVV+GGTFRAFSNSSFY +
Sbjct: 283 AASSAVISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYL 342

Query: 352 LRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVV 411
            +RIQEV+++Q  V+ C  TV+FFEK+  IYPPT N++  Y H+KKV IDLLG  +F + 
Sbjct: 343 KKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLA 402

Query: 412 PPMMGAEDFSFYTEFVPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAE 471
           P MMGAEDF+FY+E +PAAF++IG+RNE LGS+H  HSP+FMIDED LP+GAA HA +AE
Sbjct: 403 PQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAE 459

Query: 472 RYL 475
           RYL
Sbjct: 463 RYL 459

BLAST of MS015690 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 473.0 bits (1216), Expect = 2.8e-133
Identity = 221/390 (56.67%), Postives = 299/390 (76.67%), Query Frame = 0

Query: 87  LSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRAW 146
           L+LAKR +  DW+  +RRRIHENPEL +EE ETS+LVR EL++M +SY +P+A TGV  +
Sbjct: 36  LTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGY 95

Query: 147 IGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDREH 206
           +GTG  PFVALRADMDAL +QE VEWEHKS+V GKMHACGHDAH TMLLGAAK+LK+ E 
Sbjct: 96  VGTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEE 155

Query: 207 LLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAGC 266
            L+GTV+L+FQPAEE G GAK+++  G L++V AIF +HV+++   G + SR GP+LAG 
Sbjct: 156 ELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGS 215

Query: 267 GFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGNN 326
           GFF+A I+GK G A  P H++DP+LAA+  ++SLQ +VSREA+PLDSQVV+V  F GG  
Sbjct: 216 GFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGA 275

Query: 327 LDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPTV 386
            ++IPD+V IGGTFRAFS  SF Q+ +RI++VI  QASV  C+ TVDF E+E   +PPTV
Sbjct: 276 FNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTV 335

Query: 387 NDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFYIGVRNETLGSIHT 446
           ND+ +++  K V+ D+LG  N+  + P+MG+EDFSFY + +P  F ++G++N+    + +
Sbjct: 336 NDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMAS 395

Query: 447 GHSPYFMIDEDVLPIGAATHATIAERYLYE 477
            HSPYF ++E++LP GA+ HA++A RYL E
Sbjct: 396 PHSPYFEVNEELLPYGASLHASMATRYLLE 425

BLAST of MS015690 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 448.0 bits (1151), Expect = 9.7e-126
Identity = 221/398 (55.53%), Postives = 291/398 (73.12%), Query Frame = 0

Query: 79  TDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPL 138
           T      +L  AK  EV DW+ K+RR+IHENPEL +EE ETS+L+R EL+ + I Y +P+
Sbjct: 31  TSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPV 90

Query: 139 AKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAA 198
           A TGV  +IGTG PPFVALRADMDALPIQE VEWEHKS++ GKMHACGHD HVTMLLGAA
Sbjct: 91  AITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAA 150

Query: 199 KILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSR 258
           KIL +  H L+GTV+L+FQPAEE  +GAK+M  +GALK+V+AIF +H+S   P G   SR
Sbjct: 151 KILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASR 210

Query: 259 PGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSV 318
            G  LAG G F AVITGK G A  P H++DPV+AA++ V+SLQ +VSRE +PLDS+VV+V
Sbjct: 211 AGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTV 270

Query: 319 TSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKE 378
           +  NGGN  ++IPD++ IGGT RAF  + F Q+ +R++EVI +QA+V+RC+ +V+     
Sbjct: 271 SKVNGGNAFNVIPDSITIGGTLRAF--TGFTQLQQRVKEVITKQAAVHRCNASVNLTPNG 330

Query: 379 YTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFYIGVRN 438
               PPTVN++ +Y+  KKV  DLLG   F    P+MG+EDFS++ E +P  F  +G+++
Sbjct: 331 REPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQD 390

Query: 439 ETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYE 477
           ET G   + HSP + I+EDVLP GAA HA++A +YL E
Sbjct: 391 ETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKE 425

BLAST of MS015690 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 438.3 bits (1126), Expect = 7.7e-123
Identity = 213/390 (54.62%), Postives = 290/390 (74.36%), Query Frame = 0

Query: 87  LSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDELDRMEISYHHPLAKTGVRAW 146
           LSLAKR +  DW+  +RRRIHENPEL +EE ETS+LV+ ELD+M +SY +P+A TGV  +
Sbjct: 36  LSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGVSYKNPVAVTGVIGY 95

Query: 147 IGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACGHDAHVTMLLGAAKILKDREH 206
           +GTG  PFVALRADMDALPIQE VEWEHKS++ GKMHACGHDAH TMLLGAAK+LK+ + 
Sbjct: 96  VGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQE 155

Query: 207 LLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHVSHEHPTGIIGSRPGPLLAGC 266
            L+GTVIL+FQPAEE G GAK+++  G L++V AIF +HVS+    G + SR G L+AG 
Sbjct: 156 ELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGLGQLSSREGLLMAGS 215

Query: 267 GFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSREANPLDSQVVSVTSFNGGNN 326
           G F+A I+GK G A  P  ++DPVLAA+  ++SLQ +VSREA+PLDSQVV+V +F G + 
Sbjct: 216 GRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLDSQVVTVATFEGSDA 275

Query: 327 LDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVYRCSGTVDFFEKEYTIYPPTV 386
            ++IPD+V IGGTFRA    SF Q+ +RI +VI  QASV  C+ TVDF E E   +PPTV
Sbjct: 276 FNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATVDFLEDETPPFPPTV 335

Query: 387 NDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEFVPAAFFYIGVRNETLGSIHT 446
           N++ ++   K V++D+LG  N+    P+M +EDF+FY + +P  F ++G++N++   +  
Sbjct: 336 NNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMAN 395

Query: 447 GHSPYFMIDEDVLPIGAATHATIAERYLYE 477
            HSP+F ++E++LP GA+  A++A RYL +
Sbjct: 396 PHSPFFEVNEELLPYGASLLASLATRYLLD 425

BLAST of MS015690 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 436.0 bits (1120), Expect = 3.8e-122
Identity = 231/472 (48.94%), Postives = 299/472 (63.35%), Query Frame = 0

Query: 7   SGKFWIIFFISIPSFLLSFIEPISSSGGGDHGGSALIGAGCGAKPILNSASLKNRSIGTF 66
           SG F++IF          F+ P+SS+G  D G                            
Sbjct: 4   SGSFFVIF------VTFFFLPPLSSAGSYDSG---------------------------- 63

Query: 67  MSGLESQSCEVWTDACSEAILSLAKRTEVVDWLKKVRRRIHENPELAFEEFETSQLVRDE 126
            SGLES          +  +L  AK  E  +W++ +RR+IHENPE  F+EF+TSQLVRDE
Sbjct: 64  -SGLES---------LARGMLHSAKDPEFFEWMRGIRRKIHENPETGFQEFKTSQLVRDE 123

Query: 127 LDRMEISYHHPLAKTGVRAWIGTGGPPFVALRADMDALPIQEAVEWEHKSRVQGKMHACG 186
           LD + + Y +P+AKTGV AWIG+   P   LRADMDALP+QE VEWE KS+V GKMHACG
Sbjct: 124 LDSLGVKYKYPVAKTGVVAWIGSCSKPVFGLRADMDALPLQELVEWESKSKVDGKMHACG 183

Query: 187 HDAHVTMLLGAAKILKDREHLLKGTVILLFQPAEEAGNGAKRMIGDGALKDVQAIFAVHV 246
           HD HV MLLGAAK+L+  +HL+KGTV L+FQP EE   GA  M+ D  L D+  I +VHV
Sbjct: 184 HDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAGAYEMLKDEILDDLDGILSVHV 243

Query: 247 SHEHPTGIIGSRPGPLLAGCGFFRAVITGKKGRAGSPHHSVDPVLAAAAAVISLQGIVSR 306
               P+G IGSRPG +LAG G F   + G+   A +PH S DPVLAA++AV++LQ IVSR
Sbjct: 244 FPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHFSKDPVLAASSAVVALQQIVSR 303

Query: 307 EANPLDSQVVSVTSFNGGNNLDMIPDTVVIGGTFRAFSNSSFYQVLRRIQEVIVEQASVY 366
           E +PL++ VV+V    GG+  ++IP +   GGTFR+ SN     + RRI+E+   QASVY
Sbjct: 304 ELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSNDGLLFIQRRIKEISEAQASVY 363

Query: 367 RCSGTVDFFEKEYTIYPPTVNDEKMYEHVKKVAIDLLGSHNFKVVPPMMGAEDFSFYTEF 426
           RC   V+F EK+ +++P   NDE +YEH KKVA  ++G +NF   P  MG EDFSF+T+ 
Sbjct: 364 RCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMIGKNNFHDFPVTMGGEDFSFFTQK 423

Query: 427 VPAAFFYIGVRNETLGSIHTGHSPYFMIDEDVLPIGAATHATIAERYLYEHG 479
             AA F +GV+NETLG+    HSPYF +DE+ LP+GAA HA +A  YL EHG
Sbjct: 424 TKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAALHAAMAVSYLDEHG 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885213.13.5e-24288.87IAA-amino acid hydrolase ILR1-like 6 [Benincasa hispida][more]
KAG6578962.13.6e-23986.97IAA-amino acid hydrolase ILR1-like 6, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023551590.11.0e-23886.55IAA-amino acid hydrolase ILR1-like 6 [Cucurbita pepo subsp. pepo][more]
XP_022939072.14.0e-23886.55IAA-amino acid hydrolase ILR1-like 6 [Cucurbita moschata][more]
XP_022993486.14.4e-23786.13IAA-amino acid hydrolase ILR1-like 6 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VYX01.8e-17269.50IAA-amino acid hydrolase ILR1-like 6 OS=Arabidopsis thaliana OX=3702 GN=ILL6 PE=... [more]
Q5Z6784.5e-15269.15IAA-amino acid hydrolase ILR1-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8S9S43.5e-13658.78IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q84XG91.0e-13558.52IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q5N8F27.2e-13458.88IAA-amino acid hydrolase ILR1-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1FFS91.9e-23886.55IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC1114450... [more]
A0A6J1JWG32.1e-23786.13IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111489479... [more]
A0A6J1GUP66.8e-23686.55IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita moschata OX=3662 GN=LOC1114577... [more]
A0A0A0KDD21.3e-23486.22M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G384060 P... [more]
A0A6J1JS373.5e-23285.50IAA-amino acid hydrolase ILR1-like 6 OS=Cucurbita maxima OX=3661 GN=LOC111487050... [more]
Match NameE-valueIdentityDescription
AT1G44350.11.3e-17369.50IAA-leucine resistant (ILR)-like gene 6 [more]
AT1G51760.12.8e-13356.67peptidase M20/M25/M40 family protein [more]
AT5G56660.19.7e-12655.53IAA-leucine resistant (ILR)-like 2 [more]
AT1G51780.17.7e-12354.62IAA-leucine resistant (ILR)-like gene 5 [more]
AT3G02875.13.8e-12248.94Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 269..363
e-value: 7.8E-7
score: 29.0
NoneNo IPR availableGENE3D3.30.70.360coord: 264..382
e-value: 6.2E-134
score: 448.3
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 101..459
e-value: 6.2E-134
score: 448.3
NoneNo IPR availablePANTHERPTHR11014:SF62IAA-AMINO ACID HYDROLASE ILR1-LIKE 6coord: 60..477
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 93..476
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 156..471
e-value: 3.1E-35
score: 122.2
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 101..462
e-value: 1.6E-109
score: 364.3
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 60..477
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 99..474
e-value: 0.0
score: 695.219
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 265..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015690.1MS015690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0016787 hydrolase activity