MS015673 (gene) Bitter gourd (TR) v1

Overview


Name	MS015673
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	aminoacylase-1
Location	scaffold983: 187517 .. 189862 (-)
RNA-Seq Expression	MS015673
Synteny	MS015673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACCTCCATCACCACTGTTCATTCATTTCACTCGCCGCTTTTCTTCTTCTCCTTTCGTTTCCTCTCTCGTTCTGCCATCTCCAAGATGATGAAGTAGATCCAGATTCTCCAATCTCTCGCTTCCAAAACTACCTCAGAATCAAAACCGCCCACCCGAATCCGGATTACGCCTCCGCCGTCTCCTTCCTCAAATCTCAAGCACTGGCCATCGGTCTCGATGCGCAAATTCTCGAGTTCGTTCAAGGTAAACCTCTTCTCCTCTTGACCTGGCGTGGATCAAACCCTTCTCTGCCCTCAATTTTGTTAAACTCTCACATGGACTCTGTCCCCGCCGAGTCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGTCCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCATCCAGTATCTCGAAGCCATTCGGAACCTCAGGAGTCGAAATTTCGTCCCGGTTCGTTCGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTTGTTTCGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGGCAGGCATCGCCAGGAGATGAATTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGTTGATAATTAGGGCTAACGGAACCCCTGGGCATGGGTCTACATTGTACGATAACAGTGCGATGGAGAATTTGATGAAGAGTGTTGAGATTGTGAGTAGGTTTAGGGAGAGCCAATTCGATATGGTGAAGGCTGGTGAAGCTACTAATTCGGAAGTCATCTCTGTGAACCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACTGTGAGTTCGGCGAAATGAGTTGCAGGACTCAAACACAATAATAAGTCCTCTGGATATGTCTTTTTTCTTCATTAATATCGTGCTTTGTCGCAGGGATTTGTAATGAATATGCAACCTTCAGAGGCGGAAGCCGGTTTTGATCTCAGATTACCGCCCACCGCCGACCCAGATGCTATAAGAAGAAGAATTGCGGAGGAATGGGCTCCGGCTCGGCGGAATATGACGTTTCAGGTGAGTGGCGGTTGGCTGATTTGATTTCCACCACCCATTACTGATTTGATTCTGATATTCATCTGTTATTTTGAGGACCCCTGTTTTGTTAGCTCCCATTATTAGTATTCTTCGTGTGCAAAGTTTTATGCTTCTGGCATCTAAAGAATAATATGGAGAGTTCCTCTTATGACTTGTCATTTTATCTTGCTGAAAACGTAATAGAACTCCATGATCATGATTTTAAACCATTTTTTTTCTCTAAGAAACAGTTTCATTGATGTATGAAATTAGAAAAAGGGAAGAGAGTTTGCAATCCTAGGAAGGTCTAAAACCAATTTTGATATAGAAAGAATTTGAATTCTGATGGAGGAGGATGTTGTTGCTTGTTCTGTTGTTCACAGAGAGGAGATATCGGAATTTCCATTCCCCAAACAAATTGGTTCATTCCACTCGTCTTGGTAGATGAAATAGTTATATGGTTCATGACAAAATATCATATCATGAAACTCATCTACTGCATCCACAAACAATTTGCATCGGGCCCTTTATGGACATGCGGTTCACTAATTTTTCTTCCCCATGGGAGGAATTTACCAACTTTCCTATGCTTCCTTTCCACTTATCCGTGATTTGATACATTTTTAAAGCATTTATGGCCTGGAGCAATTTTATTAATTTTCTGTTTTTCTAGGGATTAGATGTTCCTCAAATAAATGCATTAATCATTATAAGTTGGGGACATCTCTAATGAATTAAACTCTTATCTATTTGTATGTCATCTTAGCAATATATGGTAGCTAAAACAGAATGGAGTTGATTCCTGCAGATTACTCAGAGAGGGCCCATCAGAGACTACTTGGGACGTCCATTAATGACGATGACAAACGGTTCAAATCCATGGTGGTCCGTATTCGAGACAGCCATCTCTGATGCTGGTGGCAAGCTTTCAAGCCCTGAAATCCTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGATCACAACGAGGTTCAACAAGTCTCCATTATTATCTCTCTCTCCATATCCATATTTTTACTCCATTTTCACTATAATCTTACTAATGGGTTGTCTCATTTGCTTATACCTTGGTGCAGTTCTTGTCAGAAACGGTCTTCTTAAGGGGCATAAAGGTTTATGAATCCATAATCAGTGCTTTGAGTTCCTTTGAAGAGGGTGCATCCCAG

mRNA sequence

ATGGCGAACCTCCATCACCACTGTTCATTCATTTCACTCGCCGCTTTTCTTCTTCTCCTTTCGTTTCCTCTCTCGTTCTGCCATCTCCAAGATGATGAAGTAGATCCAGATTCTCCAATCTCTCGCTTCCAAAACTACCTCAGAATCAAAACCGCCCACCCGAATCCGGATTACGCCTCCGCCGTCTCCTTCCTCAAATCTCAAGCACTGGCCATCGGTCTCGATGCGCAAATTCTCGAGTTCGTTCAAGGTAAACCTCTTCTCCTCTTGACCTGGCGTGGATCAAACCCTTCTCTGCCCTCAATTTTGTTAAACTCTCACATGGACTCTGTCCCCGCCGAGTCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGTCCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCATCCAGTATCTCGAAGCCATTCGGAACCTCAGGAGTCGAAATTTCGTCCCGGTTCGTTCGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTTGTTTCGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGGCAGGCATCGCCAGGAGATGAATTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGTTGATAATTAGGGCTAACGGAACCCCTGGGCATGGGTCTACATTGTACGATAACAGTGCGATGGAGAATTTGATGAAGAGTGTTGAGATTGTGAGTAGGTTTAGGGAGAGCCAATTCGATATGGTGAAGGCTGGTGAAGCTACTAATTCGGAAGTCATCTCTGTGAACCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACTGGATTTGTAATGAATATGCAACCTTCAGAGGCGGAAGCCGGTTTTGATCTCAGATTACCGCCCACCGCCGACCCAGATGCTATAAGAAGAAGAATTGCGGAGGAATGGGCTCCGGCTCGGCGGAATATGACGTTTCAGATTACTCAGAGAGGGCCCATCAGAGACTACTTGGGACGTCCATTAATGACGATGACAAACGGTTCAAATCCATGGTGGTCCGTATTCGAGACAGCCATCTCTGATGCTGGTGGCAAGCTTTCAAGCCCTGAAATCCTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGATCACAACGAGTTCTTGTCAGAAACGGTCTTCTTAAGGGGCATAAAGGTTTATGAATCCATAATCAGTGCTTTGAGTTCCTTTGAAGAGGGTGCATCCCAG

Coding sequence (CDS)

ATGGCGAACCTCCATCACCACTGTTCATTCATTTCACTCGCCGCTTTTCTTCTTCTCCTTTCGTTTCCTCTCTCGTTCTGCCATCTCCAAGATGATGAAGTAGATCCAGATTCTCCAATCTCTCGCTTCCAAAACTACCTCAGAATCAAAACCGCCCACCCGAATCCGGATTACGCCTCCGCCGTCTCCTTCCTCAAATCTCAAGCACTGGCCATCGGTCTCGATGCGCAAATTCTCGAGTTCGTTCAAGGTAAACCTCTTCTCCTCTTGACCTGGCGTGGATCAAACCCTTCTCTGCCCTCAATTTTGTTAAACTCTCACATGGACTCTGTCCCCGCCGAGTCCTCCAAGTGGCTCCATCCTCCATTCTCCGCCGTCCGGTCCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGACAAGTGCATCGCCATCCAGTATCTCGAAGCCATTCGGAACCTCAGGAGTCGAAATTTCGTCCCGGTTCGTTCGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTCGATGGAGCTGCGAAGTTTGTTTCGTCGAAGGAGTTCAAGGAGTTGAATGTGGGGTTCATGATGGACGAAGGGCAGGCATCGCCAGGAGATGAATTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGTTGATAATTAGGGCTAACGGAACCCCTGGGCATGGGTCTACATTGTACGATAACAGTGCGATGGAGAATTTGATGAAGAGTGTTGAGATTGTGAGTAGGTTTAGGGAGAGCCAATTCGATATGGTGAAGGCTGGTGAAGCTACTAATTCGGAAGTCATCTCTGTGAACCCTGTTTTCGTCAATGCCGGAATTCCTTCCCCCACTGGATTTGTAATGAATATGCAACCTTCAGAGGCGGAAGCCGGTTTTGATCTCAGATTACCGCCCACCGCCGACCCAGATGCTATAAGAAGAAGAATTGCGGAGGAATGGGCTCCGGCTCGGCGGAATATGACGTTTCAGATTACTCAGAGAGGGCCCATCAGAGACTACTTGGGACGTCCATTAATGACGATGACAAACGGTTCAAATCCATGGTGGTCCGTATTCGAGACAGCCATCTCTGATGCTGGTGGCAAGCTTTCAAGCCCTGAAATCCTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTGGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCATGATCACAACGAGTTCTTGTCAGAAACGGTCTTCTTAAGGGGCATAAAGGTTTATGAATCCATAATCAGTGCTTTGAGTTCCTTTGAAGAGGGTGCATCCCAG

Protein sequence

MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSPISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSSFEEGASQ

Homology

[Close]

BLAST of MS015673 vs. NCBI nr
Match: XP_022141532.1 (aminoacylase-1 [Momordica charantia] >XP_022141534.1 aminoacylase-1 [Momordica charantia])

HSP 1 Score: 889.8 bits (2298), Expect = 9.7e-255
Identity = 442/446 (99.10%), Postives = 444/446 (99.55%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSPISRFQNYLRIKTAHPNPDYAS 60
           MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSP+SRFQNYLRIKTAHPNPDYA 
Sbjct: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSPVSRFQNYLRIKTAHPNPDYAF 60

Query: 61  AVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH 120
           AVSFLKSQALAIGLDAQILEFV+GKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH
Sbjct: 61  AVSFLKSQALAIGLDAQILEFVEGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH 120

Query: 121 PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD 180
           PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD
Sbjct: 121 PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD 180

Query: 181 GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN 240
           GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN
Sbjct: 181 GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN 240

Query: 241 SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE 300
           SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE
Sbjct: 241 SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE 300

Query: 301 AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNP 360
           AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSN 
Sbjct: 301 AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNR 360

Query: 361 WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE 420
           WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE
Sbjct: 361 WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE 420

Query: 421 TVFLRGIKVYESIISALSSFEEGASQ 447
           TVFLRGIKVYESIISALSSFEEGASQ
Sbjct: 421 TVFLRGIKVYESIISALSSFEEGASQ 446

[Close]

BLAST of MS015673 vs. NCBI nr
Match: XP_022964785.1 (aminoacylase-1 [Cucurbita moschata])

HSP 1 Score: 781.9 bits (2018), Expect = 2.8e-222
Identity = 388/448 (86.61%), Postives = 411/448 (91.74%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN   HC   SLA FL L SFPLSF H Q  DDE D +SPISRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           L+PPFSAVR+SDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+P R+IHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRA GTPGHGS +Y
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPL+TMTN S
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF+TAISDAGGKLS PEILASTTDARFMRQLGIPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGASQ 447
            +TVFLRGIK+YESIISALSSF   ASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of MS015673 vs. NCBI nr
Match: XP_038879728.1 (aminoacylase-1-like [Benincasa hispida])

HSP 1 Score: 780.8 bits (2015), Expect = 6.3e-222
Identity = 386/447 (86.35%), Postives = 412/447 (92.17%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN H H     +AAF LLLSFPLSF H Q  D E D DSPISRFQ YLRIKTAHP+PDY
Sbjct: 1   MANFHLHYRLFPVAAFFLLLSFPLSFSHSQYGDGETDQDSPISRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAV+FL SQAL IGL+ +ILEFV GKPLLL+TW GSNPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVAFLSSQALEIGLNTRILEFVPGKPLLLVTWYGSNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           LHPPFSAVR+SDG+IFARGSQDDKCIAIQYLEAIRNL+SRNF+PVR+IHISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGRIFARGSQDDKCIAIQYLEAIRNLKSRNFIPVRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGAAKFV S+EFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLII+A GTPGHGS +Y
Sbjct: 181 FDGAAKFVLSQEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSA+ENLMKSVEIVSRFRESQF++VKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAIENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQI ++GPIRDYLG PLMTMTNGS
Sbjct: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIIEKGPIRDYLGGPLMTMTNGS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF TAIS+AGGKLS PEILA+TTDARFMRQL IPVLGFSPM NTPILLHDHNEFL
Sbjct: 361 NPWWSVFRTAISEAGGKLSKPEILATTTDARFMRQLRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGAS 446
            ++VFLRGIKVYES+ISALSSF++  S
Sbjct: 421 RDSVFLRGIKVYESLISALSSFQKDVS 447

[Close]

BLAST of MS015673 vs. NCBI nr
Match: XP_022991055.1 (aminoacylase-1 [Cucurbita maxima])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 386/448 (86.16%), Postives = 409/448 (91.29%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN   HC   SLA FL L  FPLSF H Q  DDE D +SPISRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           L+PPFSA+R+SDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+P R+IHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGA+KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRA G PGHGS +Y
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGE  NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPLMTMTN S
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF+TAISDAGGKLS PEILASTTDARFMRQLGIPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGASQ 447
            +TVFLRGIK+YESIISALSSF   ASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of MS015673 vs. NCBI nr
Match: KAG6602086.1 (Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 388/447 (86.80%), Postives = 409/447 (91.50%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN   HC   SLA FL L SF LSF H Q  DDE D +SPISRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           L+PPFSAVR+SDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVP R+IHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRA GTPGHGS +Y
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPL+TMTN S
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF+TAISDAGGKLS PEILASTTDARFMRQL IPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGAS 446
            +TVFLRGIK+YESIISALSSF   AS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of MS015673 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 5.8e-93
Identity = 177/409 (43.28%), Postives = 245/409 (59.90%), Query Frame = 0


Query: 35  DPDS---PISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLT 94
           DP+S    ++ F+ YLRI T  PNPDY  A++FL+ +A  +GL  Q +E V G  + +LT
Sbjct: 5   DPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLT 64

Query: 95  WRGSNPSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEA 154
           W G+NPSLPSILLNSH D VP     W H PF A + S+G I+ARGSQD K ++IQYLEA
Sbjct: 65  WPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEA 124

Query: 155 IRNLRSRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEF 214
           +R L+S      R+IH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F
Sbjct: 125 VRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAF 184

Query: 215 RVFYADRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAG-EAT 274
            VFY++RSPW + + + G PGH S   +++A E L K +  +  FRE +   ++A     
Sbjct: 185 TVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLK 244

Query: 275 NSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPAR 334
              V SVN   +  G+        N+ P+   A FD R+ P  D  A  +++      A 
Sbjct: 245 EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAG 304

Query: 335 RNMTFQITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARF 394
             +TF+  Q+         P MT T+ S+PWW+ F  A   A      PEI  + TD+R+
Sbjct: 305 EGVTFEFAQK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSRY 364

Query: 395 MRQLGIPVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSS 440
           +R +GIP LGFSPM+ TP+LLHDHNE L E +FLRG+ +Y  ++SAL+S
Sbjct: 365 IRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]

BLAST of MS015673 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.2e-90
Identity = 172/401 (42.89%), Postives = 240/401 (59.85%), Query Frame = 0


Query: 40  ISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSL 99
           ++ F+ YLRI T  PNPDY SAV+FL+ +A  +GL  Q +E   G  + +LTW G+NP L
Sbjct: 13  VTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGTNPLL 72

Query: 100 PSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 159
            SILLNSH D VP     W H PF A + S+G I+ARG+QD K ++IQYLEA+R L+S  
Sbjct: 73  HSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRLKSEG 132

Query: 160 FVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 219
               R+IH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERS 192

Query: 220 PWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAG-EATNSEVISVN 279
           PW + + + G PGH S   +++A E L K V  +  FRE +   ++A        V SVN
Sbjct: 193 PWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAVTSVN 252

Query: 280 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIT 339
              +  G+        N+ P+   A FD R+ P  D  A  +++      A   +TF+  
Sbjct: 253 LTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVTFEFA 312

Query: 340 QRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPV 399
           Q+         P MT T+ ++PWW+ F  A  +    L  PEI  + TD+R++R +GIP 
Sbjct: 313 QK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYIRAVGIPA 372

Query: 400 LGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSS 440
           LGFSPM+ TP+LLHDHNE L E VFLRG+ +Y  +++AL+S
Sbjct: 373 LGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of MS015673 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 334.3 bits (856), Expect = 2.1e-90
Identity = 168/400 (42.00%), Postives = 239/400 (59.75%), Query Frame = 0


Query: 40  ISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSL 99
           ++ F+ YLRI+T  P PDY +AV+FL+ +A  +GL  Q +E V G  + +LTW G+NP+L
Sbjct: 13  VTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGTNPTL 72

Query: 100 PSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 159
            SILLNSH D VP     W H PF   + +DG I+ RG+QD KC++IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEG 132

Query: 160 FVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 219
               R+IH+++VPDEE+GG  G   FV   EF+ L  GF +DEG ASP D F VFY++RS
Sbjct: 133 HHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERS 192

Query: 220 PWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNP 279
           PW L + + G PGHGS   +++A E L K +  +  FRE +   +++ +     V SVN 
Sbjct: 193 PWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVTSVNL 252

Query: 280 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQ 339
             +  G+        N+ P+   A FD R+ P  D  A   ++      A   +TF+  Q
Sbjct: 253 TMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTFEFVQ 312

Query: 340 RGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVL 399
           +           +T T+ S+PWW+ F     D    L   EI  ++TDAR++R  G+P L
Sbjct: 313 KWM------ETQVTSTDDSDPWWAAFSGVFKDMKLALEL-EICPASTDARYIRAAGVPAL 372

Query: 400 GFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSS 440
           GFSPM++TP+LLHDH+E L E VFLRG+ +Y  ++SAL+S
Sbjct: 373 GFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALAS 399

[Close]

BLAST of MS015673 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.6e-89
Identity = 166/401 (41.40%), Postives = 237/401 (59.10%), Query Frame = 0


Query: 40  ISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSL 99
           ++ F+ YLRI+T  P PDY +AV+F +  A  +GL  Q +E   G  + +LTW G+NP+L
Sbjct: 13  VTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTL 72

Query: 100 PSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 159
            SILLNSH D VP     W H PF A + S+G I+ RG+QD KC++IQYLEA++ L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYLEAVKRLKVEG 132

Query: 160 FVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 219
               R+IH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTDAFTVFYSERS 192

Query: 220 PWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAG-EATNSEVISVN 279
           PW + + + G PGH S   +++A E L K V  +  FRE ++  +++        V SVN
Sbjct: 193 PWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGSVTSVN 252

Query: 280 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIT 339
              +  G+        N+ P+   A FD R+ P  D  A   ++      A   +TF+  
Sbjct: 253 LTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQEAGEGVTFEFA 312

Query: 340 QRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPV 399
           Q+         P MT T+ ++PWW+ F  A  +    L  PEI  + TD+R++R +GIP 
Sbjct: 313 QK------FTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATDSRYIRAVGIPA 372

Query: 400 LGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSS 440
           LGFSPM+ TP+LLHDHNE L E VFLRG+ +Y  +++AL+S
Sbjct: 373 LGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

[Close]

BLAST of MS015673 vs. ExPASy Swiss-Prot
Match: Q5RFB0 (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 1.9e-88
Identity = 168/401 (41.90%), Postives = 236/401 (58.85%), Query Frame = 0


Query: 40  ISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSL 99
           ++ F+ YLRI+T  P PDY +AV+F + +A  +GL  Q +E   G  + +LTW G+NP+L
Sbjct: 13  VTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTWPGTNPTL 72

Query: 100 PSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRN 159
            SILLNSH D VP     W H PF A + S+G I+ARG+QD KCI+IQYLEA+R L+   
Sbjct: 73  SSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRRLKVEG 132

Query: 160 FVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 219
               R+IH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VFY++RS
Sbjct: 133 HRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERS 192

Query: 220 PWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAG-EATNSEVISVN 279
           PW + + + G PGH S   +++A E L K V  +  FRE ++  +++        V SVN
Sbjct: 193 PWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKEGSVTSVN 252

Query: 280 PVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQIT 339
              +  G+        N+ P+   A FD R+ P  D  A   ++      A   +T +  
Sbjct: 253 LTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGVTLEFA 312

Query: 340 QRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPV 399
           Q+         P +T T+ SNPWW+ F     D    L  PEI+ + TD R++R +GIP 
Sbjct: 313 QKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYIRAVGIPA 372

Query: 400 LGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSS 440
           LGFSPM+ TP+LLHDH+E L E VFLRG+ +Y  ++ AL+S
Sbjct: 373 LGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

[Close]

BLAST of MS015673 vs. ExPASy TrEMBL
Match: A0A6J1CK37 (aminoacylase-1 OS=Momordica charantia OX=3673 GN=LOC111011881 PE=4 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 4.7e-255
Identity = 442/446 (99.10%), Postives = 444/446 (99.55%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSPISRFQNYLRIKTAHPNPDYAS 60
           MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSP+SRFQNYLRIKTAHPNPDYA 
Sbjct: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQDDEVDPDSPVSRFQNYLRIKTAHPNPDYAF 60

Query: 61  AVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH 120
           AVSFLKSQALAIGLDAQILEFV+GKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH
Sbjct: 61  AVSFLKSQALAIGLDAQILEFVEGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLH 120

Query: 121 PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD 180
           PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD
Sbjct: 121 PPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFD 180

Query: 181 GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN 240
           GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN
Sbjct: 181 GAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDN 240

Query: 241 SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE 300
           SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE
Sbjct: 241 SAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSE 300

Query: 301 AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNP 360
           AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSN 
Sbjct: 301 AEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNR 360

Query: 361 WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE 420
           WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE
Sbjct: 361 WWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSE 420

Query: 421 TVFLRGIKVYESIISALSSFEEGASQ 447
           TVFLRGIKVYESIISALSSFEEGASQ
Sbjct: 421 TVFLRGIKVYESIISALSSFEEGASQ 446

[Close]

BLAST of MS015673 vs. ExPASy TrEMBL
Match: A0A6J1HLT6 (aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.4e-222
Identity = 388/448 (86.61%), Postives = 411/448 (91.74%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN   HC   SLA FL L SFPLSF H Q  DDE D +SPISRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           L+PPFSAVR+SDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+P R+IHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRA GTPGHGS +Y
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPL+TMTN S
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF+TAISDAGGKLS PEILASTTDARFMRQLGIPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGASQ 447
            +TVFLRGIK+YESIISALSSF   ASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of MS015673 vs. ExPASy TrEMBL
Match: A0A6J1JRT3 (aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.2e-222
Identity = 386/448 (86.16%), Postives = 409/448 (91.29%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           MAN   HC   SLA FL L  FPLSF H Q  DDE D +SPISRFQ+YLRIKTAHP+PDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAVSFL+SQAL IGL  +ILEFV GKPLLL+TW G NPSLPSILLNSHMDSVPAE SKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           L+PPFSA+R+SDGKIFARGSQDDKCIAIQYLEAIRNLRSRNF+P R+IHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
           FDGA+KFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRA G PGHGS +Y
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQFDMVKAGE  NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLR+PPTADPDAIRRRI EEWAPARRN+T QI ++GPIRDYLGRPLMTMTN S
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWWSVF+TAISDAGGKLS PEILASTTDARFMRQLGIPVLGFSPMSNTPILLH+HNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGASQ 447
            +TVFLRGIK+YESIISALSSF   ASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of MS015673 vs. ExPASy TrEMBL
Match: A0A5D3BIQ8 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001910 PE=4 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.3e-216
Identity = 372/447 (83.22%), Postives = 406/447 (90.83%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           M+N   H  F S+  +LLLLSFPLSF H Q  DDE D DSP+SRFQ YLRIKTAHP+PDY
Sbjct: 1   MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAV+FL+SQA  IGL  ++LEF  GKPLLL+TW GSNPSLPS+LLNSHMDSVPAE SKW
Sbjct: 61  ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 120

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           LHPPFSAVR+SDGKIFARGSQDDKCIAIQYLEAIRNLR+R+FVPVR++HISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEIGG 180

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
            DG AKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A GTPGHGS +Y
Sbjct: 181 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIVSRFRESQF++VKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLRLPPTADPDA+RRRIAEEWAPA RNMT+QI ++GP+RDYLG PLMTMTN S
Sbjct: 301 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 360

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWW+VF+ AISDAGGKLS PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLHDHNEFL
Sbjct: 361 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 SETVFLRGIKVYESIISALSSFEEGAS 446
           S++VF+RGIKVYES+IS LSSF+E  S
Sbjct: 421 SDSVFIRGIKVYESLISTLSSFQEDVS 447

[Close]

BLAST of MS015673 vs. ExPASy TrEMBL
Match: A0A5A7URV3 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001820 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.3e-216
Identity = 370/447 (82.77%), Postives = 407/447 (91.05%), Query Frame = 0


Query: 1   MANLHHHCSFISLAAFLLLLSFPLSFCHLQ--DDEVDPDSPISRFQNYLRIKTAHPNPDY 60
           M+N   H  F S+  +LLLLSFPLSF H Q  DDE D DSP+SRFQ YLRIKTAHP+PDY
Sbjct: 85  MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 144

Query: 61  ASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKW 120
           ASAV+FL+SQA  IGL  ++LEF  GKPLLL+TW GSNPSLPS+LLNSHMDSVPAE SKW
Sbjct: 145 ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 204

Query: 121 LHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGG 180
           LHPPFSAVR+SDGKIFARGSQDDKCIAIQY+EAIRNLR+R+FVPVR++HISYVPDEEIGG
Sbjct: 205 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEIGG 264

Query: 181 FDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLY 240
            DG AKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW LII+A GTPGHGS +Y
Sbjct: 265 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 324

Query: 241 DNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+SRFRESQF++VKAGEA NSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 325 DNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 384

Query: 301 SEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGS 360
           SEAEAGFDLRLPPTADPDA+RRRIAEEWAPA RNMT+QI ++GP+RDYLG PLMTMTN S
Sbjct: 385 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 444

Query: 361 NPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFL 420
           NPWW+VF+ AISDAGGKLS PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLHDHNEFL
Sbjct: 445 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 504

Query: 421 SETVFLRGIKVYESIISALSSFEEGAS 446
           S++VF+RGIKVYES+IS+LSSF+E  S
Sbjct: 505 SDSVFIRGIKVYESLISSLSSFQEDVS 531

[Close]

BLAST of MS015673 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 632.5 bits (1630), Expect = 2.6e-181
Identity = 294/428 (68.69%), Postives = 360/428 (84.11%), Query Frame = 0


Query: 19  LLSFPLSFCHLQDDEVDPDSPISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQI 78
           L+ F + F  LQ    + D+PI+RFQ YLR  TAHPNP+Y + +SFL +QA +IGL  + 
Sbjct: 10  LIIFSIIF-SLQSSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKT 69

Query: 79  LEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGS 138
           +EF+ GKP+LL+TW GSNP+LPSIL NSH+DSVPAES KW +PPFSA ++ DG I+ARG+
Sbjct: 70  IEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGA 129

Query: 139 QDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGF 198
           QDDKCI +QYLE+IRNL+SR F P+R+IHISYVP+EEIGGFDG  KF +S EFK+LN+GF
Sbjct: 130 QDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGF 189

Query: 199 MMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRE 258
            MDEGQA+PGDEFRVFYADR PW  +I+A G PGHG+ LYDNSAMENLMKSVE++SRFRE
Sbjct: 190 AMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRFRE 249

Query: 259 SQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAI 318
           SQFD VKAG+A  SEVISVNPV++ AG P+ TGFVMNMQPSEAEAG+DLRLPP ADPD +
Sbjct: 250 SQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPDVM 309

Query: 319 RRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSS 378
           ++RIAEEWAP+ RNMT+ I ++G +RD+LGRP+MT  N SNPWWS+F+ A+   GGKL+ 
Sbjct: 310 KKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKLAK 369

Query: 379 PEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALS 438
           PEILASTTD+RF+R LGIP  GFSPM+NTPILLHDHNEFL +TVF++GI+VYES+ISALS
Sbjct: 370 PEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISALS 429

Query: 439 SFEEGASQ 447
           SFE  ++Q
Sbjct: 430 SFEGESAQ 436

[Close]

BLAST of MS015673 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 630.2 bits (1624), Expect = 1.3e-180
Identity = 299/425 (70.35%), Postives = 357/425 (84.00%), Query Frame = 0


Query: 17  LLLLSFPLSFCHLQDDEVDPDSPISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDA 76
           L L SF  S       E + D+PI+RFQ YLR  TAHPNP+Y + +SFL  QA +IGL +
Sbjct: 10  LFLFSFIFSL-QSHSKEEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTS 69

Query: 77  QILEFVQGKPLLLLTWRGSNPSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFAR 136
           + +EFV GKP+LLLTW GSN +L SIL NSH+DSVPAES KW+HPPFSA R+ DG I+AR
Sbjct: 70  RTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHIYAR 129

Query: 137 GSQDDKCIAIQYLEAIRNLRSRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNV 196
           G+QDDKCI +QYLEAIRNL+SR+F P+R+IHISYVP+EEIGGF G  KF +S EFK+LN+
Sbjct: 130 GAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKDLNL 189

Query: 197 GFMMDEGQASPGDEFRVFYADRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRF 256
           GF+MDEGQASPGDEFRVFYA+R+PW L IRANG PGHG+ LYDNSAMENLMKSVE++SRF
Sbjct: 190 GFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVELISRF 249

Query: 257 RESQFDMVKAGEATNSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPD 316
           RESQFD VKAG+A NSEVISVNPV++ AG PS TGFVMNMQPSEAE G+DLRLPP ADP 
Sbjct: 250 RESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPMADPV 309

Query: 317 AIRRRIAEEWAPARRNMTFQITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKL 376
           A+++RIAEEWAP+ RNMT+ + Q+G + D+LGRP+MT TN +NPWWS+F+ A+   GGKL
Sbjct: 310 ALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEATGGKL 369

Query: 377 SSPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISA 436
           + PEIL STTD+RF+R LGIPVLGFSPM NTPIL+HDHNEFL +TVF++GI VYES+ISA
Sbjct: 370 AKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYESVISA 429

Query: 437 LSSFE 442
           LSSFE
Sbjct: 430 LSSFE 433

[Close]

BLAST of MS015673 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.1e-142
Identity = 232/405 (57.28%), Postives = 302/405 (74.57%), Query Frame = 0


Query: 37  DSPISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSN 96
           D+ +SRFQ YLRI T  PNP+Y  AV F+ SQA  + L++Q +EFV+GKPLLLL W GS+
Sbjct: 22  DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSD 81

Query: 97  PSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLR 156
           P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR L+
Sbjct: 82  PTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQ 141

Query: 157 SRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYA 216
           +  F P+RS+++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY 
Sbjct: 142 ASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYG 201

Query: 217 DRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVIS 276
           +RSPW L+I+A G PGHG+ LYDNSAMENL+KS+E + RFR SQFD++KAG     +V+S
Sbjct: 202 ERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVS 261

Query: 277 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQ 336
           VN  F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RNM+F+
Sbjct: 262 VNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFE 321

Query: 337 ITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGI 396
             Q+       G+  +T  + SNPWW + E A+ +AGG+ S PEI  ++TDAR+ R+ G+
Sbjct: 322 FKQK-----LTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGV 381

Query: 397 PVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSSFE 442
           P  GFSP+SNTP LLHDHNE+L +  +L+GI+VY SII A +S+E
Sbjct: 382 PAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYE 421

[Close]

BLAST of MS015673 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 1.8e-142
Identity = 232/405 (57.28%), Postives = 303/405 (74.81%), Query Frame = 0


Query: 37  DSPISRFQNYLRIKTAHPNPDYASAVSFLKSQALAIGLDAQILEFVQGKPLLLLTWRGSN 96
           D+ +SRFQ YLRI T  PNP+Y  AV F+ SQA  + L++Q +EFV+GKPLLLL W GS+
Sbjct: 22  DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSD 81

Query: 97  PSLPSILLNSHMDSVPAESSKWLHPPFSAVRSSDGKIFARGSQDDKCIAIQYLEAIRNLR 156
           P+LP+ LLNSH D VP E SKW H P  A     G I+ARGSQD KC+ +QYLEAIR L+
Sbjct: 82  PTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQ 141

Query: 157 SRNFVPVRSIHISYVPDEEIGGFDGAAKFVSSKEFKELNVGFMMDEGQASPGDEFRVFYA 216
           +  F P+RS+++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY 
Sbjct: 142 ASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYG 201

Query: 217 DRSPWGLIIRANGTPGHGSTLYDNSAMENLMKSVEIVSRFRESQFDMVKAGEATNSEVIS 276
           +RSPW L+I+A G PGHG+ LYDNSAMENL+KS+E + RFR SQFD++KAG     +V+S
Sbjct: 202 ERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVS 261

Query: 277 VNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDAIRRRIAEEWAPARRNMTFQ 336
           VN  F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RNM+F+
Sbjct: 262 VNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFE 321

Query: 337 ITQRGPIRDYLGRPLMTMTNGSNPWWSVFETAISDAGGKLSSPEILASTTDARFMRQLGI 396
           + Q    +   G+  +T  + SNPWW + E A+ +AGG+ S PEI  ++TDAR+ R+ G+
Sbjct: 322 LGQFK--QKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGV 381

Query: 397 PVLGFSPMSNTPILLHDHNEFLSETVFLRGIKVYESIISALSSFE 442
           P  GFSP+SNTP LLHDHNE+L +  +L+GI+VY SII A +S+E
Sbjct: 382 PAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASYE 424

The following BLAST results are available for this feature:

BLAST of MS015673 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141532.1	9.7e-255	99.10	aminoacylase-1 [Momordica charantia] >XP_022141534.1 aminoacylase-1 [Momordica c...	[more]
XP_022964785.1	2.8e-222	86.61	aminoacylase-1 [Cucurbita moschata]	[more]
XP_038879728.1	6.3e-222	86.35	aminoacylase-1-like [Benincasa hispida]	[more]
XP_022991055.1	1.1e-221	86.16	aminoacylase-1 [Cucurbita maxima]	[more]
KAG6602086.1	1.1e-221	86.80	Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS015673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99JW2	5.8e-93	43.28	Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1	[more]
Q6AYS7	1.2e-90	42.89	Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1	[more]
P37111	2.1e-90	42.00	Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2	[more]
Q6PTT0	6.6e-89	41.40	Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1	[more]
Q5RFB0	1.9e-88	41.90	Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2	[more]

BLAST of MS015673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CK37	4.7e-255	99.10	aminoacylase-1 OS=Momordica charantia OX=3673 GN=LOC111011881 PE=4 SV=1	[more]
A0A6J1HLT6	1.4e-222	86.61	aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1	[more]
A0A6J1JRT3	5.2e-222	86.16	aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1	[more]
A0A5D3BIQ8	4.3e-216	83.22	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00191...	[more]
A0A5A7URV3	7.3e-216	82.77	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00182...	[more]

BLAST of MS015673 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44820.1	2.6e-181	68.69	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	1.3e-180	70.35	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.1	1.1e-142	57.28	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.2	1.8e-142	57.28	Peptidase M20/M25/M40 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010159	N-acyl-L-amino-acid amidohydrolase	TIGRFAM	TIGR01880	TIGR01880	coord: 35..440 e-value: 1.9E-181 score: 601.1
None	No IPR available	PIRSF	PIRSF036696	ACY-1	coord: 29..442 e-value: 2.8E-169 score: 560.8
None	No IPR available	GENE3D	3.30.70.360		coord: 223..332 e-value: 1.4E-8 score: 36.5
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 32..222 e-value: 1.1E-52 score: 180.4
None	No IPR available	GENE3D	1.10.150.900		coord: 359..440 e-value: 2.0E-29 score: 103.1
None	No IPR available	PANTHER	PTHR45892	AMINOACYLASE-1	coord: 25..442
None	No IPR available	PANTHER	PTHR45892:SF3	PUTATIVE-RELATED	coord: 25..442
None	No IPR available	CDD	cd05646	M20_AcylaseI_like	coord: 37..438 e-value: 0.0 score: 635.852
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 34..437
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 103..435 e-value: 1.0E-33 score: 117.3
IPR011650	Peptidase M20, dimerisation domain	PFAM	PF07687	M20_dimer	coord: 219..324 e-value: 5.5E-7 score: 29.5
IPR036264	Bacterial exopeptidase dimerisation domain	SUPERFAMILY	55031	Bacterial exopeptidase dimerisation domain	coord: 223..329

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015673.1	MS015673.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004046	aminoacylase activity
molecular_function	GO:0016787	hydrolase activity