MS015461 (gene) Bitter gourd (TR) v1

Overview


Name	MS015461
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Location	scaffold424: 300984 .. 302092 (-)
RNA-Seq Expression	MS015461
Synteny	MS015461

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGAAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGGTAATCTCTTTAAGCTCTGTTTCGCGATTCGAAATCAGGAGTAGAAATTATCTGATTTGATTTGATTTGATTTGATTTGAGAGTAGAGCAGAGTTAAGTTGAGTAGTCAAGTATGTGGGATAGTGAATAATAGTTTGATTTTGTAAGTGAAATTTTGGGCAGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGATAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTAGTTTCTCATCTTTCGCCTGAATCTTCTTTCAACTTCATCCAAGTAAACCCATCATGTGGATTTTAACATAGCACCAGCAGTTGCAGATTGGTTCATTGGGGACGTTTCATCCTTTGAACTTTGATGCTTGTGTCTAAAAGTTTGATTTAAAATGTATTTTGCCCATTTTCTCTGTTCTTGTTTTATCAAGCTAGTTTGCTTCCTTGTGTAACATGTTGCCACGCGTCCTTATTCAATCCGTATGTCATTTTAACACACACAACAAAAAGAGATGTCCATTAATTATTAATTTCGTTGTTTCCGTGGGTAGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGGTAACTATA

mRNA sequence

ATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGAAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGATAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGGTAACTATA

Coding sequence (CDS)

ATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGAAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGATAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGGTAACTATA

Protein sequence

MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVTI

Homology

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BLAST of MS015461 vs. NCBI nr
Match: XP_022155250.1 (transcription factor bHLH80-like [Momordica charantia])

HSP 1 Score: 406.8 bits (1044), Expect = 1.2e-109
Identity = 208/210 (99.05%), Postives = 209/210 (99.52%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN
Sbjct: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120
           SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI
Sbjct: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120

Query: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHP 180
           SPTSKRGREVEAQNFHSKFSSQLKREESGVSSL+DMEMEKLLEDSVPCRVRAKRGCATHP
Sbjct: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180

Query: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           RSIAERVRRTKISDRIRKLQEVVPNMDK T
Sbjct: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQT 210

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BLAST of MS015461 vs. NCBI nr
Match: XP_038891266.1 (transcription factor bHLH80-like [Benincasa hispida])

HSP 1 Score: 290.0 bits (741), Expect = 1.6e-74
Identity = 167/218 (76.61%), Postives = 174/218 (79.82%), Query Frame = 0


Query: 1   MQSTTGGGTT---SSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLL 60
           MQSTTG G T   SSGGG      +GLARFRSAPA WLEALLEDDEEDPLKP+ CLTQLL
Sbjct: 1   MQSTTGAGATGSRSSGGGG----GAGLARFRSAPAAWLEALLEDDEEDPLKPSHCLTQLL 60

Query: 61  AGNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYE 120
           A NS  L SP DQ LFD    PNPSPAF RQNSSPAEFL     GEGFY+AY + S    
Sbjct: 61  AANSSDLDSPADQPLFD----PNPSPAFHRQNSSPAEFLAASGIGEGFYTAYALNS---- 120

Query: 121 LPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRA 180
             SP LD+SPTSKR REV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRA
Sbjct: 121 --SPTLDVSPTSKRAREVDAQNFSPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRA 180

Query: 181 KRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           KRGCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 181 KRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 204

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BLAST of MS015461 vs. NCBI nr
Match: XP_022925421.1 (transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription factor bHLH81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.8 bits (730), Expect = 3.0e-73
Identity = 163/215 (75.81%), Postives = 171/215 (79.53%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 1   MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFL     GEGFYS+Y + S      S
Sbjct: 61  SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 120

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKRG
Sbjct: 121 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 180

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 181 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 203

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BLAST of MS015461 vs. NCBI nr
Match: KAG6592393.1 (Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 285.8 bits (730), Expect = 3.0e-73
Identity = 163/215 (75.81%), Postives = 171/215 (79.53%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 42  MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 101

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFL     GEGFYS+Y + S      S
Sbjct: 102 SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 161

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKRG
Sbjct: 162 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 221

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 222 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 244

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BLAST of MS015461 vs. NCBI nr
Match: KAG7025210.1 (Transcription factor bHLH80, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 284.6 bits (727), Expect = 6.7e-73
Identity = 162/213 (76.06%), Postives = 170/213 (79.81%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 42  MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 101

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFL     GEGFYS+Y + S      S
Sbjct: 102 SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 161

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKRG
Sbjct: 162 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 221

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDK 209
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK
Sbjct: 222 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 242

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BLAST of MS015461 vs. ExPASy Swiss-Prot
Match: Q9C8P8 (Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 5.2e-52
Identity = 128/232 (55.17%), Postives = 158/232 (68.10%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRA--SGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQST   G +S GGG  G     SGL+R RSAPATW+E LLE+DEE+ LKPN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 61  GNSPALG------------SPPDQGLFDANPNPNPSPAFARQNSSPAEFLG------EGF 120
           GN+ + G            S  +QGL+    N +    F RQNSSPA+FL       +G+
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLY----NHHQGGGFHRQNSSPADFLSGSGSGTDGY 120

Query: 121 YSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREE--SGVSSLIDMEM 180
           +S +G+  A Y+  S  +DISPT KR R++E Q     FSSQLK E+   G+S ++DM M
Sbjct: 121 FSNFGI-PANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNM 180

Query: 181 EKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           +K+ EDSVPCRVRAKRGCATHPRSIAERVRRT+ISDRIR+LQE+VPNMDK T
Sbjct: 181 DKIFEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDKQT 221

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BLAST of MS015461 vs. ExPASy Swiss-Prot
Match: Q9M0R0 (Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 7.5e-51
Identity = 128/225 (56.89%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 4   TTGGGTTSSG-GGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSP 63
           ++GGG    G GG  G   SGL+R RSAPATWLEALLE+DEE+ LKPN  LT LL GNS 
Sbjct: 8   SSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTDLLTGNSN 67

Query: 64  AL----GS-----PPDQGLFDANPNPNPSPAFARQNSSPAEFL--GEGFYSAYGVTSAKY 123
            L    GS     P +QGL+           F RQNS+PA+FL   +GF  ++G+  A Y
Sbjct: 68  DLPTSRGSFEFPIPVEQGLYQQG-------GFHRQNSTPADFLSGSDGFIQSFGI-QANY 127

Query: 124 ELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREES------GVSSLIDMEMEKLLEDS 183
           +  S  +D+SP SKR RE+EA     +F+SQ+K E+S      GVSS+ DM ME L+EDS
Sbjct: 128 DYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMENLMEDS 187

Query: 184 VPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           V  RVRAKRGCATHPRSIAERVRRT+ISDRIRKLQE+VPNMDK T
Sbjct: 188 VAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQT 224

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BLAST of MS015461 vs. ExPASy Swiss-Prot
Match: Q66GR3 (Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-18
Identity = 64/174 (36.78%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 40  EDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQN-SSPAEFLGE 99
           E+DEE P   N         + P    P   G+    P   P   F   + + P+ F+  
Sbjct: 161 EEDEESPSNSNGLRRHCSLSSRP----PSSLGMLSQIPEIAPETNFPYSHWNDPSSFID- 220

Query: 100 GFYSAYGVTSAKYELPSPGLDI--SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDM 159
                  ++S K E    G     +   + G  ++  + H      L +  S  S ++ +
Sbjct: 221 ------NLSSLKREAEDDGKLFLGAQNGESGNRMQLLSHH----LSLPKSSSTASDMVSV 280

Query: 160 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           +    L+DSVPC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQE+VPNMDK T
Sbjct: 281 DKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQT 319

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BLAST of MS015461 vs. ExPASy Swiss-Prot
Match: Q9C690 (Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.2e-17
Identity = 41/54 (75.93%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 157 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           ++E+LL DS+PC++RAKRGCATHPRSIAERVRRTKIS+R+RKLQ++VPNMD  T
Sbjct: 291 DIEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQT 344

[Close]

BLAST of MS015461 vs. ExPASy Swiss-Prot
Match: Q8H102 (Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.9e-15
Identity = 77/214 (35.98%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 7   GGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGS 66
           GG  SSG       +  LAR RS+PA +   L  D      K N  L Q  +  SP  GS
Sbjct: 129 GGGNSSG-------SYSLARQRSSPADFFTYLASD------KNNFSLNQPTSDYSPQGGS 188

Query: 67  PPDQGLFDAN-----PNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYE-----LPSP 126
              +G           N +        N +P        +SA    +A  +       S 
Sbjct: 189 NGGRGHSRLKSQLSFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSI 248

Query: 127 GLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRGC 186
           G  ++  SKR +++++  F     SQ         + +D  M+ L EDSVPC++RAKRGC
Sbjct: 249 GFTVTRPSKRSKDMDSGLF-----SQYSLPSDTSMNYMDNFMQ-LPEDSVPCKIRAKRGC 308

Query: 187 ATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           ATHPRSIAER RRT+IS +++KLQ++VPNMDK T
Sbjct: 309 ATHPRSIAERERRTRISGKLKKLQDLVPNMDKQT 323

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BLAST of MS015461 vs. ExPASy TrEMBL
Match: A0A6J1DLX6 (transcription factor bHLH80-like OS=Momordica charantia OX=3673 GN=LOC111022390 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 5.7e-110
Identity = 208/210 (99.05%), Postives = 209/210 (99.52%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN
Sbjct: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120
           SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI
Sbjct: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120

Query: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRGCATHP 180
           SPTSKRGREVEAQNFHSKFSSQLKREESGVSSL+DMEMEKLLEDSVPCRVRAKRGCATHP
Sbjct: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180

Query: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           RSIAERVRRTKISDRIRKLQEVVPNMDK T
Sbjct: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQT 210

[Close]

BLAST of MS015461 vs. ExPASy TrEMBL
Match: A0A6J1EC61 (transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.5e-73
Identity = 163/215 (75.81%), Postives = 171/215 (79.53%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 1   MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFL     GEGFYS+Y + S      S
Sbjct: 61  SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 120

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKRG
Sbjct: 121 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 180

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 181 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 203

[Close]

BLAST of MS015461 vs. ExPASy TrEMBL
Match: A0A6J1ICS6 (transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.6e-72
Identity = 161/215 (74.88%), Postives = 169/215 (78.60%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S   G       GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 1   MQSTTGGATGSRSSG------GGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFL     GEGFYS+Y + S      S
Sbjct: 61  SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 120

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKRG
Sbjct: 121 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 180

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 181 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 199

[Close]

BLAST of MS015461 vs. ExPASy TrEMBL
Match: A0A1S3CBY9 (transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.9e-68
Identity = 156/216 (72.22%), Postives = 164/216 (75.93%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTG   T++          GLARFRSAPA WLEALLEDDEEDPLKPN CLTQLLA N
Sbjct: 1   MQSTTGAAATATASSRTSGGGPGLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLAAN 60

Query: 61  SPALGSPP-DQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELP 120
           S  L S P D  LFD    PNPSPAF RQNSSP EFL      EGFY++Y + S      
Sbjct: 61  SSDLVSAPGDHPLFD----PNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNS------ 120

Query: 121 SPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKR 180
           SP LDISPTSK   +V+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKR
Sbjct: 121 SPTLDISPTSKPATDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKR 180

Query: 181 GCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           GCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK T
Sbjct: 181 GCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQT 206

[Close]

BLAST of MS015461 vs. ExPASy TrEMBL
Match: A0A5D3BMS8 (Transcription factor bHLH80 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00070 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.4e-68
Identity = 155/214 (72.43%), Postives = 163/214 (76.17%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTG   T++          GLARFRSAPA WLEALLEDDEEDPLKPN CLTQLLA N
Sbjct: 1   MQSTTGAAATATASSRTSGGGPGLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLAAN 60

Query: 61  SPALGSPP-DQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYELP 120
           S  L S P D  LFD    PNPSPAF RQNSSP EFL      EGFY++Y + S      
Sbjct: 61  SSDLDSAPGDHPLFD----PNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNS------ 120

Query: 121 SPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKR 180
           SP LDISPTSK   +V+AQNF  KFS QLKRE SGVSSLIDMEMEKLLEDSVPCRVRAKR
Sbjct: 121 SPTLDISPTSKPATDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKR 180

Query: 181 GCATHPRSIAERVRRTKISDRIRKLQEVVPNMDK 209
           GCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDK
Sbjct: 181 GCATHPRSIAERVRRTRISDRIRKLQEVVPNMDK 204

[Close]

BLAST of MS015461 vs. TAIR 10
Match: AT1G35460.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 3.7e-53
Identity = 128/232 (55.17%), Postives = 158/232 (68.10%), Query Frame = 0


Query: 1   MQSTTGGGTTSSGGGDLGPRA--SGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQST   G +S GGG  G     SGL+R RSAPATW+E LLE+DEE+ LKPN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 61  GNSPALG------------SPPDQGLFDANPNPNPSPAFARQNSSPAEFLG------EGF 120
           GN+ + G            S  +QGL+    N +    F RQNSSPA+FL       +G+
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLY----NHHQGGGFHRQNSSPADFLSGSGSGTDGY 120

Query: 121 YSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREE--SGVSSLIDMEM 180
           +S +G+  A Y+  S  +DISPT KR R++E Q     FSSQLK E+   G+S ++DM M
Sbjct: 121 FSNFGI-PANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNM 180

Query: 181 EKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           +K+ EDSVPCRVRAKRGCATHPRSIAERVRRT+ISDRIR+LQE+VPNMDK T
Sbjct: 181 DKIFEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDKQT 221

[Close]

BLAST of MS015461 vs. TAIR 10
Match: AT4G09180.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 5.3e-52
Identity = 128/225 (56.89%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 4   TTGGGTTSSG-GGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSP 63
           ++GGG    G GG  G   SGL+R RSAPATWLEALLE+DEE+ LKPN  LT LL GNS 
Sbjct: 8   SSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTDLLTGNSN 67

Query: 64  AL----GS-----PPDQGLFDANPNPNPSPAFARQNSSPAEFL--GEGFYSAYGVTSAKY 123
            L    GS     P +QGL+           F RQNS+PA+FL   +GF  ++G+  A Y
Sbjct: 68  DLPTSRGSFEFPIPVEQGLYQQG-------GFHRQNSTPADFLSGSDGFIQSFGI-QANY 127

Query: 124 ELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREES------GVSSLIDMEMEKLLEDS 183
           +  S  +D+SP SKR RE+EA     +F+SQ+K E+S      GVSS+ DM ME L+EDS
Sbjct: 128 DYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMENLMEDS 187

Query: 184 VPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           V  RVRAKRGCATHPRSIAERVRRT+ISDRIRKLQE+VPNMDK T
Sbjct: 188 VAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQT 224

[Close]

BLAST of MS015461 vs. TAIR 10
Match: AT2G42280.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.5e-19
Identity = 64/174 (36.78%), Postives = 90/174 (51.72%), Query Frame = 0


Query: 40  EDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQN-SSPAEFLGE 99
           E+DEE P   N         + P    P   G+    P   P   F   + + P+ F+  
Sbjct: 161 EEDEESPSNSNGLRRHCSLSSRP----PSSLGMLSQIPEIAPETNFPYSHWNDPSSFID- 220

Query: 100 GFYSAYGVTSAKYELPSPGLDI--SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDM 159
                  ++S K E    G     +   + G  ++  + H      L +  S  S ++ +
Sbjct: 221 ------NLSSLKREAEDDGKLFLGAQNGESGNRMQLLSHH----LSLPKSSSTASDMVSV 280

Query: 160 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           +    L+DSVPC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQE+VPNMDK T
Sbjct: 281 DKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQT 319

[Close]

BLAST of MS015461 vs. TAIR 10
Match: AT1G51140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 3.0e-18
Identity = 41/54 (75.93%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 157 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           ++E+LL DS+PC++RAKRGCATHPRSIAERVRRTKIS+R+RKLQ++VPNMD  T
Sbjct: 291 DIEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQT 344

[Close]

BLAST of MS015461 vs. TAIR 10
Match: AT1G05805.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-16
Identity = 77/214 (35.98%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 7   GGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGS 66
           GG  SSG       +  LAR RS+PA +   L  D      K N  L Q  +  SP  GS
Sbjct: 129 GGGNSSG-------SYSLARQRSSPADFFTYLASD------KNNFSLNQPTSDYSPQGGS 188

Query: 67  PPDQGLFDAN-----PNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYE-----LPSP 126
              +G           N +        N +P        +SA    +A  +       S 
Sbjct: 189 NGGRGHSRLKSQLSFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSI 248

Query: 127 GLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLIDMEMEKLLEDSVPCRVRAKRGC 186
           G  ++  SKR +++++  F     SQ         + +D  M+ L EDSVPC++RAKRGC
Sbjct: 249 GFTVTRPSKRSKDMDSGLF-----SQYSLPSDTSMNYMDNFMQ-LPEDSVPCKIRAKRGC 308

Query: 187 ATHPRSIAERVRRTKISDRIRKLQEVVPNMDKVT 211
           ATHPRSIAER RRT+IS +++KLQ++VPNMDK T
Sbjct: 309 ATHPRSIAERERRTRISGKLKKLQDLVPNMDKQT 323

The following BLAST results are available for this feature:

BLAST of MS015461 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155250.1	1.2e-109	99.05	transcription factor bHLH80-like [Momordica charantia]	[more]
XP_038891266.1	1.6e-74	76.61	transcription factor bHLH80-like [Benincasa hispida]	[more]
XP_022925421.1	3.0e-73	75.81	transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription f...	[more]
KAG6592393.1	3.0e-73	75.81	Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma ...	[more]
KAG7025210.1	6.7e-73	76.06	Transcription factor bHLH80, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of MS015461 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8P8	5.2e-52	55.17	Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1	[more]
Q9M0R0	7.5e-51	56.89	Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1	[more]
Q66GR3	4.9e-18	36.78	Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=...	[more]
Q9C690	4.2e-17	75.93	Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=...	[more]
Q8H102	3.9e-15	35.98	Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=...	[more]

BLAST of MS015461 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLX6	5.7e-110	99.05	transcription factor bHLH80-like OS=Momordica charantia OX=3673 GN=LOC111022390 ...	[more]
A0A6J1EC61	1.5e-73	75.81	transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 S...	[more]
A0A6J1ICS6	3.6e-72	74.88	transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=...	[more]
A0A1S3CBY9	1.9e-68	72.22	transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 P...	[more]
A0A5D3BMS8	2.4e-68	72.43	Transcription factor bHLH80 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MS015461 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35460.1	3.7e-53	55.17	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G09180.1	5.3e-52	56.89	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G42280.1	3.5e-19	36.78	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G51140.1	3.0e-18	75.93	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G05805.1	2.8e-16	35.98	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..90
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	PANTHER	PTHR16223:SF225	TRANSCRIPTION FACTOR BHLH80	coord: 12..210
None	No IPR available	PANTHER	PTHR16223	TRANSCRIPTION FACTOR BHLH83-RELATED	coord: 12..210
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 176..211 score: 9.406878
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 179..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS015461.1	MS015461.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046983	protein dimerization activity