MS015444 (gene) Bitter gourd (TR) v1

Overview
NameMS015444
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Description2-isopropylmalate synthase
Locationscaffold424: 61273 .. 64723 (+)
RNA-Seq ExpressionMS015444
SyntenyMS015444
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACGTTTGTATTGCCTCCTGCTTCAATTTTCTTTAAGTGAACCGCTGGATTTTTCTACAACTGAAGCTTCGTTCATTGAGTTAAACAAAGAAATTAATCGGTTGGGATTCGAGGGAAGTTTTGGGGACTTTTGTGGTCTGGCAGATTTTTTGTTCAAAGAACTTGATTTGAGGTTCAAGCGAGTTTTCCATGATTTGAGAAGTATTTCGTTCAGCCGTTCTCCTGAACTTGCTCGTAGAGATAATGATTTGTGGGACACCATTAAAGAGTTTATGTTGCTCTTAAGAAGTTGTTTGGTTATCTTGACTCTGGTTGCTTTCGAACAAAATGTTCTACTGGAAAAGGGCGGTGTTCTTCTTTCAGTAGTCAGGAAATTGCTAGATTTGATTACAAGTGGAAAGGATGAGAAAGAGTCCATCAGTTTGGAGAAATCTTTTTTACACGAATGCAGAATCACTAAATCTGATTGTACTACCTTTGTCTCTGAGGATGTTGTTGCTTCCTTGTGTATTTTAGAACCCTCCGATCCATGCCATCCATTCATATGTGCAGTGCTTGAGGTTCTTACAGGATTTCCCTGCTCTTTTTGAACTTATTATTCTTTCCCTGCTTTTTACTGCATTTTCACCTTTTCTCCCTTGCATGAATTTGTTTTTGGATAAGTTGTCTATCGATTACCATGATCTGAAAAGATTTGATATTTTGTTATCTTTACCAATTGGATCTCAATGTTGTAGGCATTTTTTAAGTAACTCGTATGCACGTACTCACGTAAAGAATGTTGTGTTTTGCCAAATTTTATCATTTAGTTCACAATTATTATTTATCAAGAATTTCTTTGGCTGGATCATCAACTTGTTGGCGTGCTTTAGAGTTTTTATGTGAATGGAAAATTTCCTTGATCAAAAGATTGGGCTGCTATGACCAGTTAAGGGAATCATCTGAGCTCTGATGTTGTCTGTCTTCGCCCACCATTCATCTTGAAAATCTCTGTATGTTCACATCATATCATTTTGATTCTTAGTGGGTTTCAGCAGCTCATGATTGTCTGGCATACAATGTTCACCTTGTTGTGGTAAACTTCATGTGTTAATTTGTAGACTCAATTTTTTTTTTTTTCATTTAGGGGTTGGTAATAAATAATAAAACTTAATTTGGACTAATAATTCTTAGGTATTTGTGGATGAGCTTCTAATGCATAGATCACTGAGAGAATACTTTATGCTGGTAGATTCTGCATCTTCTACTACTAAAATGGTATTTATGCACAACTTAGTTCATGGTGGAATCGGGACGGTGCTGGAGGTGATTTCTGCTCATTTCATCCTGTCAGTTTCTGATGAGCAGGCATTTCATAATTTCCTCAATAGACTATGTTGGGCACACTATGAGGATTTAAGAGTTCCTGAATTAAGCCTGACAACTGCTTTATCATTGCATATGAATCCTGTCATGCTATCTGCACCAAAATTGTTCCAAGCTCATTTTATTTCACTCGTTTCTGAAGTCATTGGCATCGGTGTATTCTTGAAATCCCCAAACCCAGATCATAGGCTTATGGACTGGTACCTTGTAGCATTTGAGAGGGCAATCACGTTGTATAATAGACATATGTCTAATTCACATGCGGAAGATGCTCCATTTAATTCCAATGGCTGTTTTTCCAGTTCAGGTGTGCCTCGGAATAGTGGTCAGCAACCTTTTGAATCTTACATTCAAAAGGTTAGGAGAGAACAAATTGACAATCTTACTTCCAAGTATGAGAACACTTGTTTGCTTTTGAGGGAAAAATCAGAGCTTCTAGCCTTATCGATTTCGTATGTAGTGGAGAACCAACATATTCTTGATGAATCACATAAAGATGATCTTTTGTCAATCTTACATTGTATAATTCTTGGAGCTTCTCAGGATGATGTTCATGACATCGAAATTTACAAGACTGGGTATACAAGTCACCATGACATATATCTTCTTGCTTCTATATTGAAGTTGATGAGTTCATCAATGTTGCCTGCTATTTGGTGTCTAAGGCACTGTAGAAACTCTGGTGGCCTTAAAATCTTACGAGATGTTTCATCTTGTAAGGAGTATGGTTTTATTTTGAGCATCATCAACTGTTTTCAAGAATTTGATATTTCCCTACCAAATCAGAATCTCATATCTAAAGTGATGAAATCTCACCCAAAGAGGCATAAGAATTCCAAGTGGATGTTCCTGCATTTTACAGGACTACTGGCGTTAAGCTTTACGAGAGGGTTTGACTTCTTAGTGAAAGACTGTGTACTTGCAATTATGACAACATTGAATTTGTTTGTTTTTGAAGAGGGAGATTTAGATGCATTATCATCATTAATTGGCTCTGAGACAGCTGAAGAGGTACGTTTTATTTTATTTTTAATGACTTTGTTTGCTGTGCTATTTAATTTTTCTTCTATTTTATTATTACTTTGAATTACTGCTTTTAATTATTGACATATCCTACAGGGCTTGCTACACCTAAAATATGGTGAAACAGTTGCAATGAAGTTTCAAAAGGTTCAGACAATTCACATGAGGTAGGAGGCACTTTTTAAAATATCTTTGCAAATTTTCACATATCAACAAAATGAAACTTTCTCATCTTTTGGGTTCATTTTACTTTGTTTTTCATATAATTCTGTCAATGGGTATCTGAATTCAGTCCCATCTCTTTATGAAAATGGGTTAAACTGTTTTGACCTTTAATTGCAAAGTCTATGAAGCTCTCTTTTACATACTTGTTTTTGCTTCCTTATCTCTCTTTCATTGTTGGTTTTCTTCAATAGAGAGTTTCCATGGCCTAAAGAATATTTCAAACTTTTATCTTCTTGACATTGCACAAGCACGAAATGAGAAAAACGTTAAATTGAAAACCTGAAGTCTGCAGCTGACTGGATTAAATTGTTCTCCTCTGTACCATTAGCTTTGTTGTAAGATGTTTAAGTTGTATTTCTTATTAGACAGCACTGGTTGCTGCTTGTATTTTTTCAGTTGGGAAATCCTTCATTTTTGTGACTTTACCATGTCAGGAAGATCTGGCTGGAATGGAATGTTTTGACCATACTTTACCATGATAAATTTTTTCAACCTTTATCTTGCAGCACGGATTCTCTCACAAGCTGCCATCAAAGAATACGCGATGATTCGCAAACCCCTCCCATCCATAATAGTCTAAAGGAATGTACTGTTGGTGTAGAGGAGGCGGAAGAAACTTGCAGCGGGGAGATATTTCTAAAATGTTTGCTAGAAGACTCCAAGGACTCTTCATCTTATGACGATATAGCAGACTTTGTAGAGTGCAAGCGTGGGAAGGATTATTTGCAGTGGATGAAGAATAAACAAAGATATCGAAAATGGAAATCACAGAAATTGGCAGTATTGAGATGGAAGAAAAAGAAAAAGATTTGGAAATGTATCAAAACAAAAAAT

mRNA sequence

ATGAAACGTTTGTATTGCCTCCTGCTTCAATTTTCTTTAAGTGAACCGCTGGATTTTTCTACAACTGAAGCTTCGTTCATTGAGTTAAACAAAGAAATTAATCGGTTGGGATTCGAGGGAAGTTTTGGGGACTTTTGTGGTCTGGCAGATTTTTTGTTCAAAGAACTTGATTTGAGGTTCAAGCGAGTTTTCCATGATTTGAGAAGTATTTCGTTCAGCCGTTCTCCTGAACTTGCTCGTAGAGATAATGATTTGTGGGACACCATTAAAGAGTTTATGTTGCTCTTAAGAAGTTGTTTGGTTATCTTGACTCTGGTTGCTTTCGAACAAAATGTTCTACTGGAAAAGGGCGGTGTTCTTCTTTCAGTAGTCAGGAAATTGCTAGATTTGATTACAAGTGGAAAGGATGAGAAAGAGTCCATCAGTTTGGAGAAATCTTTTTTACACGAATGCAGAATCACTAAATCTGATTGTACTACCTTTGTCTCTGAGGATGTTGTTGCTTCCTTGTGTATTTTAGAACCCTCCGATCCATGCCATCCATTCATATGTGCAGTGCTTGAGGTATTTGTGGATGAGCTTCTAATGCATAGATCACTGAGAGAATACTTTATGCTGGTAGATTCTGCATCTTCTACTACTAAAATGGTATTTATGCACAACTTAGTTCATGGTGGAATCGGGACGGTGCTGGAGGTGATTTCTGCTCATTTCATCCTGTCAGTTTCTGATGAGCAGGCATTTCATAATTTCCTCAATAGACTATGTTGGGCACACTATGAGGATTTAAGAGTTCCTGAATTAAGCCTGACAACTGCTTTATCATTGCATATGAATCCTGTCATGCTATCTGCACCAAAATTGTTCCAAGCTCATTTTATTTCACTCGTTTCTGAAGTCATTGGCATCGGTGTATTCTTGAAATCCCCAAACCCAGATCATAGGCTTATGGACTGGTACCTTGTAGCATTTGAGAGGGCAATCACGTTGTATAATAGACATATGTCTAATTCACATGCGGAAGATGCTCCATTTAATTCCAATGGCTGTTTTTCCAGTTCAGGTGTGCCTCGGAATAGTGGTCAGCAACCTTTTGAATCTTACATTCAAAAGGTTAGGAGAGAACAAATTGACAATCTTACTTCCAAGTATGAGAACACTTGTTTGCTTTTGAGGGAAAAATCAGAGCTTCTAGCCTTATCGATTTCGTATGTAGTGGAGAACCAACATATTCTTGATGAATCACATAAAGATGATCTTTTGTCAATCTTACATTGTATAATTCTTGGAGCTTCTCAGGATGATGTTCATGACATCGAAATTTACAAGACTGGGTATACAAGTCACCATGACATATATCTTCTTGCTTCTATATTGAAGTTGATGAGTTCATCAATGTTGCCTGCTATTTGGTGTCTAAGGCACTGTAGAAACTCTGGTGGCCTTAAAATCTTACGAGATGTTTCATCTTGTAAGGAGTATGGTTTTATTTTGAGCATCATCAACTGTTTTCAAGAATTTGATATTTCCCTACCAAATCAGAATCTCATATCTAAAGTGATGAAATCTCACCCAAAGAGGCATAAGAATTCCAAGTGGATGTTCCTGCATTTTACAGGACTACTGGCGTTAAGCTTTACGAGAGGGTTTGACTTCTTAGTGAAAGACTGTGTACTTGCAATTATGACAACATTGAATTTGTTTGTTTTTGAAGAGGGAGATTTAGATGCATTATCATCATTAATTGGCTCTGAGACAGCTGAAGAGGGCTTGCTACACCTAAAATATGGTGAAACAGTTGCAATGAAGTTTCAAAAGGTTCAGACAATTCACATGAGCACGGATTCTCTCACAAGCTGCCATCAAAGAATACGCGATGATTCGCAAACCCCTCCCATCCATAATAGTCTAAAGGAATGTACTGTTGGTGTAGAGGAGGCGGAAGAAACTTGCAGCGGGGAGATATTTCTAAAATGTTTGCTAGAAGACTCCAAGGACTCTTCATCTTATGACGATATAGCAGACTTTGTAGAGTGCAAGCGTGGGAAGGATTATTTGCAGTGGATGAAGAATAAACAAAGATATCGAAAATGGAAATCACAGAAATTGGCAGTATTGAGATGGAAGAAAAAGAAAAAGATTTGGAAATGTATCAAAACAAAAAAT

Coding sequence (CDS)

ATGAAACGTTTGTATTGCCTCCTGCTTCAATTTTCTTTAAGTGAACCGCTGGATTTTTCTACAACTGAAGCTTCGTTCATTGAGTTAAACAAAGAAATTAATCGGTTGGGATTCGAGGGAAGTTTTGGGGACTTTTGTGGTCTGGCAGATTTTTTGTTCAAAGAACTTGATTTGAGGTTCAAGCGAGTTTTCCATGATTTGAGAAGTATTTCGTTCAGCCGTTCTCCTGAACTTGCTCGTAGAGATAATGATTTGTGGGACACCATTAAAGAGTTTATGTTGCTCTTAAGAAGTTGTTTGGTTATCTTGACTCTGGTTGCTTTCGAACAAAATGTTCTACTGGAAAAGGGCGGTGTTCTTCTTTCAGTAGTCAGGAAATTGCTAGATTTGATTACAAGTGGAAAGGATGAGAAAGAGTCCATCAGTTTGGAGAAATCTTTTTTACACGAATGCAGAATCACTAAATCTGATTGTACTACCTTTGTCTCTGAGGATGTTGTTGCTTCCTTGTGTATTTTAGAACCCTCCGATCCATGCCATCCATTCATATGTGCAGTGCTTGAGGTATTTGTGGATGAGCTTCTAATGCATAGATCACTGAGAGAATACTTTATGCTGGTAGATTCTGCATCTTCTACTACTAAAATGGTATTTATGCACAACTTAGTTCATGGTGGAATCGGGACGGTGCTGGAGGTGATTTCTGCTCATTTCATCCTGTCAGTTTCTGATGAGCAGGCATTTCATAATTTCCTCAATAGACTATGTTGGGCACACTATGAGGATTTAAGAGTTCCTGAATTAAGCCTGACAACTGCTTTATCATTGCATATGAATCCTGTCATGCTATCTGCACCAAAATTGTTCCAAGCTCATTTTATTTCACTCGTTTCTGAAGTCATTGGCATCGGTGTATTCTTGAAATCCCCAAACCCAGATCATAGGCTTATGGACTGGTACCTTGTAGCATTTGAGAGGGCAATCACGTTGTATAATAGACATATGTCTAATTCACATGCGGAAGATGCTCCATTTAATTCCAATGGCTGTTTTTCCAGTTCAGGTGTGCCTCGGAATAGTGGTCAGCAACCTTTTGAATCTTACATTCAAAAGGTTAGGAGAGAACAAATTGACAATCTTACTTCCAAGTATGAGAACACTTGTTTGCTTTTGAGGGAAAAATCAGAGCTTCTAGCCTTATCGATTTCGTATGTAGTGGAGAACCAACATATTCTTGATGAATCACATAAAGATGATCTTTTGTCAATCTTACATTGTATAATTCTTGGAGCTTCTCAGGATGATGTTCATGACATCGAAATTTACAAGACTGGGTATACAAGTCACCATGACATATATCTTCTTGCTTCTATATTGAAGTTGATGAGTTCATCAATGTTGCCTGCTATTTGGTGTCTAAGGCACTGTAGAAACTCTGGTGGCCTTAAAATCTTACGAGATGTTTCATCTTGTAAGGAGTATGGTTTTATTTTGAGCATCATCAACTGTTTTCAAGAATTTGATATTTCCCTACCAAATCAGAATCTCATATCTAAAGTGATGAAATCTCACCCAAAGAGGCATAAGAATTCCAAGTGGATGTTCCTGCATTTTACAGGACTACTGGCGTTAAGCTTTACGAGAGGGTTTGACTTCTTAGTGAAAGACTGTGTACTTGCAATTATGACAACATTGAATTTGTTTGTTTTTGAAGAGGGAGATTTAGATGCATTATCATCATTAATTGGCTCTGAGACAGCTGAAGAGGGCTTGCTACACCTAAAATATGGTGAAACAGTTGCAATGAAGTTTCAAAAGGTTCAGACAATTCACATGAGCACGGATTCTCTCACAAGCTGCCATCAAAGAATACGCGATGATTCGCAAACCCCTCCCATCCATAATAGTCTAAAGGAATGTACTGTTGGTGTAGAGGAGGCGGAAGAAACTTGCAGCGGGGAGATATTTCTAAAATGTTTGCTAGAAGACTCCAAGGACTCTTCATCTTATGACGATATAGCAGACTTTGTAGAGTGCAAGCGTGGGAAGGATTATTTGCAGTGGATGAAGAATAAACAAAGATATCGAAAATGGAAATCACAGAAATTGGCAGTATTGAGATGGAAGAAAAAGAAAAAGATTTGGAAATGTATCAAAACAAAAAAT

Protein sequence

MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRFKRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVLLSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCHPFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFILSVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEVIGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNSGQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDLLSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSGGLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTGLLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETVAMKFQKVQTIHMSTDSLTSCHQRIRDDSQTPPIHNSLKECTVGVEEAEETCSGEIFLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWKCIKTKN
Homology
BLAST of MS015444 vs. NCBI nr
Match: XP_022155241.1 (uncharacterized protein LOC111022382 [Momordica charantia])

HSP 1 Score: 1412.5 bits (3655), Expect = 0.0e+00
Identity = 715/726 (98.48%), Postives = 718/726 (98.90%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDL F
Sbjct: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLSF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVFHDLRSISFSRSPELARRDNDLWDTIKEF+LLLRSCLVILTLVAFEQNVLLEKGGVL
Sbjct: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFILLLRSCLVILTLVAFEQNVLLEKGGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH
Sbjct: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           IGIGVFLKSPNPDHRLMDWYLVAFE AITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS
Sbjct: 301 IGIGVFLKSPNPDHRLMDWYLVAFETAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL
Sbjct: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLP IWCLRHCRNSG
Sbjct: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPVIWCLRHCRNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG
Sbjct: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV
Sbjct: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSLKECTVGVEEAEETCSGEIF 660
           AMKFQKVQTIHMSTDSLTSCHQRIRDD     +TPPIHNSL+ECTVGVEEAEETCSGEIF
Sbjct: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDDLGQSVETPPIHNSLRECTVGVEEAEETCSGEIF 660

Query: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720
           LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK
Sbjct: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720

Query: 721 CIKTKN 723
           CIKTKN
Sbjct: 721 CIKTKN 726

BLAST of MS015444 vs. NCBI nr
Match: XP_022998345.1 (uncharacterized protein LOC111493012 [Cucurbita maxima])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 594/728 (81.59%), Postives = 650/728 (89.29%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL  S  EA+ +ELNKE ++ G   SF D C LADFLFKELD RF
Sbjct: 1   MKRLYCLLLQWSLTEPLGLSRNEAALVELNKEFSQSGSRKSFRDLCDLADFLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DLR+ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+ EK GVL
Sbjct: 61  KRVFYDLRTISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVHEKVGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTF+SED VASLCILEPSDPCH
Sbjct: 121 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFISEDFVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDS SSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHGSLREYFMLVDSTSSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA YEDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLYWAQYEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GI VF+KSPNPDHRLMD +LVAFERAI +YNR MSNS+ +DAP NSNGCFS+S VP +S
Sbjct: 301 VGIAVFVKSPNPDHRLMDRFLVAFERAILMYNRRMSNSYLKDAPLNSNGCFSNSSVPWSS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQ+VRRE++DNL SKYENTCL LREKSELL L+ SYV ENQHI DE HKDD 
Sbjct: 361 GQQPFESYIQQVRREKVDNLISKYENTCLFLREKSELLTLASSYVEENQHIFDEQHKDDF 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKTGY SHHDI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 421 LSILHCIVLGASQVDV-DTKIYKTGYASHHDIFLLASILKLMSSSLLPAVWCLRHHGNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFILSII+CF+EFD+SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 481 GLKTLRDVSSSTEYGFILSIISCFEEFDVSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGFDFLVKDCVLAIMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 541 LLALSFSRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL--KECTVGVEEAEETCSGE 660
           A++FQK+QT  M TDSL SCH+R+ DD     +T PIHN+   KEC +G+EEAEETCSGE
Sbjct: 601 AVEFQKIQTFQMRTDSLKSCHRRMHDDLGLSMETAPIHNNTCGKECDIGIEEAEETCSGE 660

Query: 661 IFLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKI 720
           IFLKCLL D K SSSY DI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+I
Sbjct: 661 IFLKCLLADPKGSSSYGDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRI 720

Query: 721 WKCIKTKN 723
           WKC+KTKN
Sbjct: 721 WKCMKTKN 727

BLAST of MS015444 vs. NCBI nr
Match: XP_023525754.1 (uncharacterized protein LOC111789268 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 595/727 (81.84%), Postives = 650/727 (89.41%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL  S  EA+ ++LNKEI + G   SF D C LADFLFKELD RF
Sbjct: 1   MKRLYCLLLQWSLTEPLGLSRNEAALVKLNKEIGQSGSCKSFRDLCDLADFLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DL +ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+LEK GVL
Sbjct: 61  KRVFYDLITISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVLEKVGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTFVSED VASLCILEPSDPCH
Sbjct: 121 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFVSEDFVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDS SSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHESLREYFMLVDSTSSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA YEDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLYWAQYEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GIGVF+KSPNPDHRLMD YLVAFERAI +YNRHMSNS+ +DAP NSNGCFS+S VP +S
Sbjct: 301 VGIGVFVKSPNPDHRLMDRYLVAFERAIMMYNRHMSNSYLKDAPLNSNGCFSNSSVPWSS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQ+VRRE++DNL SKYENTCL  REKSELLAL+ SYV ENQHI DE HKDD 
Sbjct: 361 GQQPFESYIQQVRREKVDNLISKYENTCLFFREKSELLALASSYVEENQHIFDEQHKDDF 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKT   SH+DI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 421 LSILHCIVLGASQVDV-DTKIYKTRNASHYDIFLLASILKLMSSSLLPAVWCLRHHGNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFI SII+CF+EFD+SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 481 GLKTLRDVSSRTEYGFISSIISCFEEFDVSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGFDFLVKDCVLAIMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 541 LLALSFSRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL-KECTVGVEEAEETCSGEI 660
           A++FQK+QT  M TDSL SCH+R+ DD     +T PIHN+  KEC +G+EEAEETCSGEI
Sbjct: 601 AVEFQKIQTFQMRTDSLKSCHRRMHDDLGQSMETAPIHNTCGKECDIGIEEAEETCSGEI 660

Query: 661 FLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIW 720
           FLKCLL D K SSSYDDI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+IW
Sbjct: 661 FLKCLLADPKGSSSYDDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRIW 720

Query: 721 KCIKTKN 723
           KC+KTKN
Sbjct: 721 KCMKTKN 726

BLAST of MS015444 vs. NCBI nr
Match: XP_022949475.1 (uncharacterized protein LOC111452808 [Cucurbita moschata])

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 593/727 (81.57%), Postives = 649/727 (89.27%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL FS  EA+ +ELNKE  + G   SF D C LADFLFKELD RF
Sbjct: 1   MKRLYCLLLQWSLTEPLGFSRNEAALVELNKEFCQSGSGKSFRDLCDLADFLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DLR+ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+LEK GVL
Sbjct: 61  KRVFYDLRTISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVLEKVGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTFVSE+ VASLCILEPSDPCH
Sbjct: 121 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFVSENFVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDS SSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHESLREYFMLVDSTSSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA YEDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLYWAQYEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GIGVF+KSPNPDHRLMD YLVAFERAI +YNRHMSNS+ +DAP NSNGCFS+S VP +S
Sbjct: 301 VGIGVFVKSPNPDHRLMDRYLVAFERAIMMYNRHMSNSYLKDAPLNSNGCFSNSSVPWSS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQ+VRRE++DNL SKYENTCL  REKSELLAL+ SYV E+QHI DE HKDD 
Sbjct: 361 GQQPFESYIQQVRREKVDNLISKYENTCLFFREKSELLALASSYVEESQHIFDEQHKDDF 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKT    H+DI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 421 LSILHCIVLGASQVDV-DTKIYKTRNAGHYDIFLLASILKLMSSSLLPAVWCLRHHGNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFI SII+CF+EFD SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 481 GLKTLRDVSSSTEYGFISSIISCFEEFDFSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGFDFLVKDCVLAIMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 541 LLALSFSRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL-KECTVGVEEAEETCSGEI 660
           A++F+K+QT  M TDSL SCH+R+ DD     +T PIHN+  KEC +G+EEAEETCSGEI
Sbjct: 601 AVEFKKIQTFQMRTDSLKSCHRRMHDDLGQSMETAPIHNTCGKECDIGIEEAEETCSGEI 660

Query: 661 FLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIW 720
           FLKCLL D K SSSYDDI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+IW
Sbjct: 661 FLKCLLADPKGSSSYDDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRIW 720

Query: 721 KCIKTKN 723
           KC+KTKN
Sbjct: 721 KCMKTKN 726

BLAST of MS015444 vs. NCBI nr
Match: KAG6607491.1 (hypothetical protein SDJN03_00833, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 590/721 (81.83%), Postives = 646/721 (89.60%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL  S  EA+ +ELNKE ++ G   SF D C LADFLFKELD RF
Sbjct: 29  MKRLYCLLLQWSLTEPLGLSRNEAALVELNKEFSQSGSRKSFRDLCDLADFLFKELDRRF 88

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DLR+ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+LEK GVL
Sbjct: 89  KRVFYDLRTISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVLEKVGVL 148

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTFVSED VASLCILEPSDPCH
Sbjct: 149 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFVSEDFVASLCILEPSDPCH 208

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 209 PFICAVLEVFVDELLMHGSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 268

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA +EDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 269 SVSDEQAFHNFLNRLYWAQFEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 328

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GIGVF+KSPNPDHRLMD YLVAFERAI +YNRHMSNS+ +DA  NSNGCFS+S VP +S
Sbjct: 329 VGIGVFVKSPNPDHRLMDRYLVAFERAIMMYNRHMSNSYLKDASLNSNGCFSNSSVPWSS 388

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPF SYIQ+VRRE++DNL SKYENTCL LREKSELLAL+ SYV ENQHI DE HKDD 
Sbjct: 389 GQQPFGSYIQQVRREKVDNLISKYENTCLFLREKSELLALASSYVEENQHIFDEQHKDDF 448

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKTGY S HDI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 449 LSILHCIVLGASQVDV-DTKIYKTGYASPHDIFLLASILKLMSSSLLPAVWCLRHHGNSG 508

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFILSII+CF+EFD+SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 509 GLKTLRDVSSSTEYGFILSIISCFEEFDVSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 568

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGF+FLVKDCVL IMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 569 LLALSFSRGFEFLVKDCVLGIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 628

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL-KECTVGVEEAEETCSGEI 660
           A++F+K+QT  M TDSL SCH+R+ DD     +T PIHN+  KEC +G+EEAEETCSGEI
Sbjct: 629 AVEFKKIQTFQMRTDSLKSCHRRMHDDLGQSMETAPIHNTCGKECDIGIEEAEETCSGEI 688

Query: 661 FLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIW 717
           FLKCLL D K SSSYDDI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+IW
Sbjct: 689 FLKCLLADPKGSSSYDDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRIW 748

BLAST of MS015444 vs. ExPASy TrEMBL
Match: A0A6J1DR48 (uncharacterized protein LOC111022382 OS=Momordica charantia OX=3673 GN=LOC111022382 PE=4 SV=1)

HSP 1 Score: 1412.5 bits (3655), Expect = 0.0e+00
Identity = 715/726 (98.48%), Postives = 718/726 (98.90%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDL F
Sbjct: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLSF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVFHDLRSISFSRSPELARRDNDLWDTIKEF+LLLRSCLVILTLVAFEQNVLLEKGGVL
Sbjct: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFILLLRSCLVILTLVAFEQNVLLEKGGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH
Sbjct: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           IGIGVFLKSPNPDHRLMDWYLVAFE AITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS
Sbjct: 301 IGIGVFLKSPNPDHRLMDWYLVAFETAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL
Sbjct: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLP IWCLRHCRNSG
Sbjct: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPVIWCLRHCRNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG
Sbjct: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV
Sbjct: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSLKECTVGVEEAEETCSGEIF 660
           AMKFQKVQTIHMSTDSLTSCHQRIRDD     +TPPIHNSL+ECTVGVEEAEETCSGEIF
Sbjct: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDDLGQSVETPPIHNSLRECTVGVEEAEETCSGEIF 660

Query: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720
           LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK
Sbjct: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720

Query: 721 CIKTKN 723
           CIKTKN
Sbjct: 721 CIKTKN 726

BLAST of MS015444 vs. ExPASy TrEMBL
Match: A0A6J1KC87 (uncharacterized protein LOC111493012 OS=Cucurbita maxima OX=3661 GN=LOC111493012 PE=4 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 594/728 (81.59%), Postives = 650/728 (89.29%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL  S  EA+ +ELNKE ++ G   SF D C LADFLFKELD RF
Sbjct: 1   MKRLYCLLLQWSLTEPLGLSRNEAALVELNKEFSQSGSRKSFRDLCDLADFLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DLR+ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+ EK GVL
Sbjct: 61  KRVFYDLRTISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVHEKVGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTF+SED VASLCILEPSDPCH
Sbjct: 121 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFISEDFVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDS SSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHGSLREYFMLVDSTSSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA YEDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLYWAQYEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GI VF+KSPNPDHRLMD +LVAFERAI +YNR MSNS+ +DAP NSNGCFS+S VP +S
Sbjct: 301 VGIAVFVKSPNPDHRLMDRFLVAFERAILMYNRRMSNSYLKDAPLNSNGCFSNSSVPWSS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQ+VRRE++DNL SKYENTCL LREKSELL L+ SYV ENQHI DE HKDD 
Sbjct: 361 GQQPFESYIQQVRREKVDNLISKYENTCLFLREKSELLTLASSYVEENQHIFDEQHKDDF 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKTGY SHHDI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 421 LSILHCIVLGASQVDV-DTKIYKTGYASHHDIFLLASILKLMSSSLLPAVWCLRHHGNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFILSII+CF+EFD+SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 481 GLKTLRDVSSSTEYGFILSIISCFEEFDVSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGFDFLVKDCVLAIMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 541 LLALSFSRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL--KECTVGVEEAEETCSGE 660
           A++FQK+QT  M TDSL SCH+R+ DD     +T PIHN+   KEC +G+EEAEETCSGE
Sbjct: 601 AVEFQKIQTFQMRTDSLKSCHRRMHDDLGLSMETAPIHNNTCGKECDIGIEEAEETCSGE 660

Query: 661 IFLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKI 720
           IFLKCLL D K SSSY DI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+I
Sbjct: 661 IFLKCLLADPKGSSSYGDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRI 720

Query: 721 WKCIKTKN 723
           WKC+KTKN
Sbjct: 721 WKCMKTKN 727

BLAST of MS015444 vs. ExPASy TrEMBL
Match: A0A6J1GCV5 (uncharacterized protein LOC111452808 OS=Cucurbita moschata OX=3662 GN=LOC111452808 PE=4 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 593/727 (81.57%), Postives = 649/727 (89.27%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKRLYCLLLQ+SL+EPL FS  EA+ +ELNKE  + G   SF D C LADFLFKELD RF
Sbjct: 1   MKRLYCLLLQWSLTEPLGFSRNEAALVELNKEFCQSGSGKSFRDLCDLADFLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           KRVF+DLR+ISFS SPE AR+DNDLW++IKEFMLLLRSCLVILTLVAFEQNV+LEK GVL
Sbjct: 61  KRVFYDLRTISFSISPEHARKDNDLWNSIKEFMLLLRSCLVILTLVAFEQNVVLEKVGVL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LITSGK+EKESISLEK FLHECRIT+ DCTTFVSE+ VASLCILEPSDPCH
Sbjct: 121 LSVLRKLLYLITSGKEEKESISLEKYFLHECRITEPDCTTFVSENFVASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMH SLREYFMLVDS SSTTKMVFMHNLVHGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHESLREYFMLVDSTSSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVSDEQAFHNFLNRL WA YEDLRVPELSLTTALSL +NPV+LSAPKLFQAHFISLVSEV
Sbjct: 241 SVSDEQAFHNFLNRLYWAQYEDLRVPELSLTTALSLLLNPVILSAPKLFQAHFISLVSEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
           +GIGVF+KSPNPDHRLMD YLVAFERAI +YNRHMSNS+ +DAP NSNGCFS+S VP +S
Sbjct: 301 VGIGVFVKSPNPDHRLMDRYLVAFERAIMMYNRHMSNSYLKDAPLNSNGCFSNSSVPWSS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
           GQQPFESYIQ+VRRE++DNL SKYENTCL  REKSELLAL+ SYV E+QHI DE HKDD 
Sbjct: 361 GQQPFESYIQQVRREKVDNLISKYENTCLFFREKSELLALASSYVEESQHIFDEQHKDDF 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQ DV D +IYKT    H+DI+LLASILKLMSSS+LPA+WCLRH  NSG
Sbjct: 421 LSILHCIVLGASQVDV-DTKIYKTRNAGHYDIFLLASILKLMSSSLLPAVWCLRHHGNSG 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK LRDVSS  EYGFI SII+CF+EFD SLPNQNLISKVMKSHP+RHKNSKWMFLHFTG
Sbjct: 481 GLKTLRDVSSSTEYGFISSIISCFEEFDFSLPNQNLISKVMKSHPERHKNSKWMFLHFTG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+RGFDFLVKDCVLAIMTTLNLFVFEEGDLDALS L GSE A+EGL+ LK  ETV
Sbjct: 541 LLALSFSRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSLLGGSENAKEGLIDLKSSETV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDD----SQTPPIHNSL-KECTVGVEEAEETCSGEI 660
           A++F+K+QT  M TDSL SCH+R+ DD     +T PIHN+  KEC +G+EEAEETCSGEI
Sbjct: 601 AVEFKKIQTFQMRTDSLKSCHRRMHDDLGQSMETAPIHNTCGKECDIGIEEAEETCSGEI 660

Query: 661 FLKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIW 720
           FLKCLL D K SSSYDDI DFVECKRGK+Y+QWMKN+QRYRKWKSQKLAVLRWKKKK+IW
Sbjct: 661 FLKCLLADPKGSSSYDDITDFVECKRGKNYMQWMKNRQRYRKWKSQKLAVLRWKKKKRIW 720

Query: 721 KCIKTKN 723
           KC+KTKN
Sbjct: 721 KCMKTKN 726

BLAST of MS015444 vs. ExPASy TrEMBL
Match: A0A5A7T6X6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00230 PE=4 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 573/726 (78.93%), Postives = 625/726 (86.09%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKR+Y LLLQ SL E  D S TEA+  ELN E  R GF+ S  DF GLAD LFKELD RF
Sbjct: 71  MKRVYYLLLQLSLGEQFDLSRTEAALNELNHEFGRFGFKESSRDFLGLADVLFKELDRRF 130

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           K VF DLR++SFS SPE+A +DNDLWDTI+EFMLLLRSCLVI+TLV FEQ+ LLEK G L
Sbjct: 131 KGVFRDLRNVSFSPSPEVAHKDNDLWDTIEEFMLLLRSCLVIMTLVEFEQDALLEKAGFL 190

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LIT+GK+EKESISLEKSFLHEC+IT SDCTTFVSED+ ASLCILEPSDPCH
Sbjct: 191 LSVLRKLLHLITTGKEEKESISLEKSFLHECKITGSDCTTFVSEDLFASLCILEPSDPCH 250

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMHRSLREYFMLVDSASST KMVF+HNL HGGIGTVLEVISAHFIL
Sbjct: 251 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTNKMVFVHNLDHGGIGTVLEVISAHFIL 310

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVS++QAFHNFLNRL WA Y DL+VPELSLT+ALSL +NPVMLSAPKLFQAHFISLV EV
Sbjct: 311 SVSNQQAFHNFLNRLYWARYGDLKVPELSLTSALSLLLNPVMLSAPKLFQAHFISLVCEV 370

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
             IGVFLKSPNPD RLMDWYLV FE+AI LYNRH+SNS+ +D P NSNGCFS   VP NS
Sbjct: 371 --IGVFLKSPNPDQRLMDWYLVGFEKAIMLYNRHISNSYMKDTPLNSNGCFSDPSVPWNS 430

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
            QQPFESYI +VRRE+I++L+SKYENTCL  REKSELLALSISYV EN+HILDES +DD 
Sbjct: 431 AQQPFESYIHQVRREKINHLSSKYENTCLFFREKSELLALSISYVEENRHILDESLEDDP 490

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQDDV+D EIYK GYTS +DIYLLASILKLMSSS LPAI CLRH  NS 
Sbjct: 491 LSILHCILLGASQDDVNDTEIYKAGYTSQYDIYLLASILKLMSSSFLPAIRCLRHHGNSD 550

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK L DVSS KEY FILSII+CFQEFDI LPNQNLIS+VMKSHPKRHKNSKWMFLHF G
Sbjct: 551 GLKTLLDVSSSKEYSFILSIISCFQEFDIFLPNQNLISEVMKSHPKRHKNSKWMFLHFIG 610

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+ G D LVKDCVLAIM TLNLFVFEEGDLDALSSLIGSE A+EG+ HLK  +TV
Sbjct: 611 LLALSFSIGSDILVKDCVLAIMATLNLFVFEEGDLDALSSLIGSEKAKEGVYHLKSSKTV 670

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDDS----QTPPIHNSLKECTVGVEEAEETCSGEIF 660
           AM+FQK+QTIH+ST SL SCH RIRD+S    +T  IH+S KE ++ VEEAEET SGEIF
Sbjct: 671 AMEFQKIQTIHVSTTSLESCHHRIRDESGQWLETAAIHSSEKESSIEVEEAEETWSGEIF 730

Query: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720
           LKCL      SSS+DDI DFVECKRGKDY QWMKN+Q+YRKWKS KLAVLRWKKKK IWK
Sbjct: 731 LKCL-----GSSSHDDIVDFVECKRGKDYSQWMKNRQKYRKWKSHKLAVLRWKKKKHIWK 789

Query: 721 CIKTKN 723
           CIKTKN
Sbjct: 791 CIKTKN 789

BLAST of MS015444 vs. ExPASy TrEMBL
Match: A0A1S3CA85 (uncharacterized protein LOC103498717 OS=Cucumis melo OX=3656 GN=LOC103498717 PE=4 SV=1)

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 572/726 (78.79%), Postives = 624/726 (85.95%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEGSFGDFCGLADFLFKELDLRF 60
           MKR+Y LLLQ SL E  D S TEA+  ELN E  R GF+ S  DF GLAD LFKELD RF
Sbjct: 1   MKRVYYLLLQLSLGEQFDLSRTEAALNELNHEFGRFGFKESSRDFLGLADVLFKELDRRF 60

Query: 61  KRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLLEKGGVL 120
           K VF DLR++SFS SPE+A +DNDLWDTI+EFMLLLRSCLVI+TLV FEQ+ LLEK G L
Sbjct: 61  KGVFRDLRNVSFSPSPEVAHKDNDLWDTIEEFMLLLRSCLVIMTLVEFEQDALLEKAGFL 120

Query: 121 LSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILEPSDPCH 180
           LSV+RKLL LIT+GK+EKESISLEKSFLHEC+IT SDCTTFVSED+ ASLCILEPSDPCH
Sbjct: 121 LSVLRKLLHLITTGKEEKESISLEKSFLHECKITGSDCTTFVSEDLFASLCILEPSDPCH 180

Query: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVISAHFIL 240
           PFICAVLEVFVDELLMHRSLREYFMLVDSASST KMVF+HNL HGGIGTVLEVISAHFIL
Sbjct: 181 PFICAVLEVFVDELLMHRSLREYFMLVDSASSTNKMVFVHNLDHGGIGTVLEVISAHFIL 240

Query: 241 SVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFISLVSEV 300
           SVS++QAFHNFLNRL WA Y DL+VPELSLT+ALSL +NPVMLSAPKLFQAHFISLV EV
Sbjct: 241 SVSNQQAFHNFLNRLYWARYGDLKVPELSLTSALSLLLNPVMLSAPKLFQAHFISLVCEV 300

Query: 301 IGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSSGVPRNS 360
             IGVFLKSPNPD RLMDWYLV FE+AI LYNRH+SNS+ +D P NSNGCFS   VP NS
Sbjct: 301 --IGVFLKSPNPDQRLMDWYLVGFEKAIVLYNRHISNSYMKDTPLNSNGCFSDPSVPWNS 360

Query: 361 GQQPFESYIQKVRREQIDNLTSKYENTCLLLREKSELLALSISYVVENQHILDESHKDDL 420
            QQPFESYI +VRRE+I++L+SKYENTCL  REKSELLALSISYV EN+HILDES +DD 
Sbjct: 361 AQQPFESYIHQVRREKINHLSSKYENTCLFFREKSELLALSISYVEENRHILDESLEDDP 420

Query: 421 LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSSSMLPAIWCLRHCRNSG 480
           LSILHCI+LGASQDDV+D EIYK GYTS +DIYLLASILKLMSSS LPAI CLRH  NS 
Sbjct: 421 LSILHCILLGASQDDVNDTEIYKAGYTSQYDIYLLASILKLMSSSFLPAIRCLRHHGNSD 480

Query: 481 GLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMKSHPKRHKNSKWMFLHFTG 540
           GLK L DVSS KEY FILSII+CFQEFDI LPNQNLIS+VMKSHPKRHKNSKWMFLHF G
Sbjct: 481 GLKTLLDVSSSKEYSFILSIISCFQEFDIFLPNQNLISEVMKSHPKRHKNSKWMFLHFIG 540

Query: 541 LLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLIGSETAEEGLLHLKYGETV 600
           LLALSF+ G D LVKDCVLAIM TLNLFVFEEGDLDALSSLIGS  A+EG+ HLK  +TV
Sbjct: 541 LLALSFSIGSDILVKDCVLAIMATLNLFVFEEGDLDALSSLIGS--AKEGVYHLKSSKTV 600

Query: 601 AMKFQKVQTIHMSTDSLTSCHQRIRDDS----QTPPIHNSLKECTVGVEEAEETCSGEIF 660
           AM+FQK+QTIH+ST SL SCH RIRD+S    +T  IH+S KE ++ VEEAEET SGEIF
Sbjct: 601 AMEFQKIQTIHVSTTSLESCHHRIRDESGQWLETAAIHSSEKESSIEVEEAEETWSGEIF 660

Query: 661 LKCLLEDSKDSSSYDDIADFVECKRGKDYLQWMKNKQRYRKWKSQKLAVLRWKKKKKIWK 720
           LKCL      SSS+DDI DFVECKRGKDY QWMKN+Q+YRKWKS KLAVLRWKKKK IWK
Sbjct: 661 LKCL-----GSSSHDDIVDFVECKRGKDYSQWMKNRQKYRKWKSHKLAVLRWKKKKHIWK 717

Query: 721 CIKTKN 723
           CIKTKN
Sbjct: 721 CIKTKN 717

BLAST of MS015444 vs. TAIR 10
Match: AT4G27510.1 (unknown protein; Has 25 Blast hits to 23 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 25; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 352.4 bits (903), Expect = 8.4e-97
Identity = 251/756 (33.20%), Postives = 394/756 (52.12%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEG------SFGDFCGLADFLFK 60
           +KRL CLL   SL++P ++   + +    N     + F G      +F D C L+D LF 
Sbjct: 40  LKRLLCLLGPLSLTQPTNWENCDIASHHWN-----IKFRGEKVDLINFDDVCFLSDVLFT 99

Query: 61  ELDLRFKRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLL 120
           ELD   + +F  L    F +  E         D+I+   L L+ C+ I++L+  +Q ++L
Sbjct: 100 ELDRSLESLFATL----FKQKAETCPTFESTEDSIELASLFLKCCMKIMSLLVAKQELVL 159

Query: 121 EKGGVLLSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILE 180
           EK   LLS++ +L+                       R    DC+   + D   SL    
Sbjct: 160 EKAKTLLSILGRLI-----------------------RARNGDCSFVFTHD--GSL---- 219

Query: 181 PSDPCHPFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVI 240
             DP H F+C  +EVF+DE+L+++S+ +   +VDSA S+ ++   H+        V++++
Sbjct: 220 --DPRHTFLCTGIEVFMDEILVNKSISDLLFVVDSAFSSCRLFSKHDR-----AGVVQIV 279

Query: 241 SAHFILSVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFI 300
           SAHFI++ SDE+     + RL W      R P++SL+ A+SL +NPVM SAPK+  A+ +
Sbjct: 280 SAHFIIATSDEKTNQMCVERLYWKKGNAFRTPQISLSAAVSLLLNPVMFSAPKMIHAYVV 339

Query: 301 SLVSEVIGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSS 360
            LVS+ IGI         D +L+D Y+ AFE+++ LY RHMS S         NG     
Sbjct: 340 LLVSDAIGICSHPCFKGLDLQLIDHYIDAFEKSVVLYKRHMSKS--------ENGLSGKF 399

Query: 361 GVPRNSGQQPFESYIQKVRREQIDNLTSKYENTC------LLLREKSELLALSISYVVEN 420
           G   +  +  FE  +      +++++T + +++          RE +EL+A S++Y  E+
Sbjct: 400 GFLTSKSRVAFEHRLLPSTLAKVNDVTQQLKDSWDSYQSDNAKRENNELVAYSVAYAKES 459

Query: 421 QHILDESHKDDL----LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSS 480
             + D S  + +    LSIL C+IL AS DDV D  + K   +S  D+YLLAS LK M+ 
Sbjct: 460 LCVFDSSCSESMLSQTLSILGCVILRASSDDVMDSVLEKYNTSSMEDLYLLASTLKFMTC 519

Query: 481 SMLPAIWCLR--HCRNSGGLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMK 540
           SML AI  LR  +   +GG     DV +CKEY  ++ ++  F++F+I +P Q+ +   M+
Sbjct: 520 SMLQAIRVLRNWNWHEAGG-----DVRACKEYKAMMDVVQRFEQFNIHMPCQSFLRDRME 579

Query: 541 SHPKRHKNSKWMFLHFTGLLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLI 600
           S+P R   SKWM +HF+GLL++SF    DFLVKD +  ++ ++ LF+ E GDL+AL   +
Sbjct: 580 SYPHR---SKWMLMHFSGLLSVSFALKLDFLVKDSIFGMVISMYLFILEGGDLEALGDSV 639

Query: 601 G-------------------SETAEEGLLHLKYGETVAMKFQKVQTIHMSTDSLTSCHQR 660
           G                   S  A++  +  K    VA+KF K +T+++   S     + 
Sbjct: 640 GHSENTSSSILSSGSKNLAASGKADKTAVDRKQSGAVALKFHKNRTLYLGKVSEAKGPEN 699

Query: 661 IRDDSQTPPIHNSLKECTVGVEEAEETCSGEIFLKCLL-EDSKDSSSYDDIADFVECKRG 717
             D               VGVE  EETC+GE FL C+  + +   +  D++ADF+ C  G
Sbjct: 700 GSDSG-------------VGVE--EETCNGERFLWCMAGKGNVTKTDVDELADFIACDPG 719

BLAST of MS015444 vs. TAIR 10
Match: AT4G27510.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 12 growth stages; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 300.8 bits (769), Expect = 2.9e-81
Identity = 212/649 (32.67%), Postives = 338/649 (52.08%), Query Frame = 0

Query: 1   MKRLYCLLLQFSLSEPLDFSTTEASFIELNKEINRLGFEG------SFGDFCGLADFLFK 60
           +KRL CLL   SL++P ++   + +    N     + F G      +F D C L+D LF 
Sbjct: 40  LKRLLCLLGPLSLTQPTNWENCDIASHHWN-----IKFRGEKVDLINFDDVCFLSDVLFT 99

Query: 61  ELDLRFKRVFHDLRSISFSRSPELARRDNDLWDTIKEFMLLLRSCLVILTLVAFEQNVLL 120
           ELD   + +F  L    F +  E         D+I+   L L+ C+ I++L+  +Q ++L
Sbjct: 100 ELDRSLESLFATL----FKQKAETCPTFESTEDSIELASLFLKCCMKIMSLLVAKQELVL 159

Query: 121 EKGGVLLSVVRKLLDLITSGKDEKESISLEKSFLHECRITKSDCTTFVSEDVVASLCILE 180
           EK   LLS++ +L+                       R    DC+   + D   SL    
Sbjct: 160 EKAKTLLSILGRLI-----------------------RARNGDCSFVFTHD--GSL---- 219

Query: 181 PSDPCHPFICAVLEVFVDELLMHRSLREYFMLVDSASSTTKMVFMHNLVHGGIGTVLEVI 240
             DP H F+C  +EVF+DE+L+++S+ +   +VDSA S+ ++   H+        V++++
Sbjct: 220 --DPRHTFLCTGIEVFMDEILVNKSISDLLFVVDSAFSSCRLFSKHDR-----AGVVQIV 279

Query: 241 SAHFILSVSDEQAFHNFLNRLCWAHYEDLRVPELSLTTALSLHMNPVMLSAPKLFQAHFI 300
           SAHFI++ SDE+     + RL W      R P++SL+ A+SL +NPVM SAPK+  A+ +
Sbjct: 280 SAHFIIATSDEKTNQMCVERLYWKKGNAFRTPQISLSAAVSLLLNPVMFSAPKMIHAYVV 339

Query: 301 SLVSEVIGIGVFLKSPNPDHRLMDWYLVAFERAITLYNRHMSNSHAEDAPFNSNGCFSSS 360
            LVS+ IGI         D +L+D Y+ AFE+++ LY RHMS S         NG     
Sbjct: 340 LLVSDAIGICSHPCFKGLDLQLIDHYIDAFEKSVVLYKRHMSKS--------ENGLSGKF 399

Query: 361 GVPRNSGQQPFESYIQKVRREQIDNLTSKYENTC------LLLREKSELLALSISYVVEN 420
           G   +  +  FE  +      +++++T + +++          RE +EL+A S++Y  E+
Sbjct: 400 GFLTSKSRVAFEHRLLPSTLAKVNDVTQQLKDSWDSYQSDNAKRENNELVAYSVAYAKES 459

Query: 421 QHILDESHKDDL----LSILHCIILGASQDDVHDIEIYKTGYTSHHDIYLLASILKLMSS 480
             + D S  + +    LSIL C+IL AS DDV D  + K   +S  D+YLLAS LK M+ 
Sbjct: 460 LCVFDSSCSESMLSQTLSILGCVILRASSDDVMDSVLEKYNTSSMEDLYLLASTLKFMTC 519

Query: 481 SMLPAIWCLR--HCRNSGGLKILRDVSSCKEYGFILSIINCFQEFDISLPNQNLISKVMK 540
           SML AI  LR  +   +GG     DV +CKEY  ++ ++  F++F+I +P Q+ +   M+
Sbjct: 520 SMLQAIRVLRNWNWHEAGG-----DVRACKEYKAMMDVVQRFEQFNIHMPCQSFLRDRME 579

Query: 541 SHPKRHKNSKWMFLHFTGLLALSFTRGFDFLVKDCVLAIMTTLNLFVFEEGDLDALSSLI 600
           S+P R   SKWM +HF+GLL++SF    DFLVKD +  ++ ++ LF+ E GDL+AL   +
Sbjct: 580 SYPHR---SKWMLMHFSGLLSVSFALKLDFLVKDSIFGMVISMYLFILEGGDLEALGDSV 627

Query: 601 G-------------------SETAEEGLLHLKYGETVAMKFQKVQTIHM 613
           G                   S  A++  +  K    VA+KF K +T+++
Sbjct: 640 GHSENTSSSILSSGSKNLAASGKADKTAVDRKQSGAVALKFHKNRTLYL 627

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155241.10.0e+0098.48uncharacterized protein LOC111022382 [Momordica charantia][more]
XP_022998345.10.0e+0081.59uncharacterized protein LOC111493012 [Cucurbita maxima][more]
XP_023525754.10.0e+0081.84uncharacterized protein LOC111789268 [Cucurbita pepo subsp. pepo][more]
XP_022949475.10.0e+0081.57uncharacterized protein LOC111452808 [Cucurbita moschata][more]
KAG6607491.10.0e+0081.83hypothetical protein SDJN03_00833, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DR480.0e+0098.48uncharacterized protein LOC111022382 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1KC870.0e+0081.59uncharacterized protein LOC111493012 OS=Cucurbita maxima OX=3661 GN=LOC111493012... [more]
A0A6J1GCV50.0e+0081.57uncharacterized protein LOC111452808 OS=Cucurbita moschata OX=3662 GN=LOC1114528... [more]
A0A5A7T6X60.0e+0078.93Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3CA850.0e+0078.79uncharacterized protein LOC103498717 OS=Cucumis melo OX=3656 GN=LOC103498717 PE=... [more]
Match NameE-valueIdentityDescription
AT4G27510.18.4e-9733.20unknown protein; Has 25 Blast hits to 23 proteins in 8 species: Archae - 0; Bact... [more]
AT4G27510.22.9e-8132.67unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR367862-ISOPROPYLMALATE SYNTHASEcoord: 1..721

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015444.1MS015444.1mRNA