MS015288 (gene) Bitter gourd (TR) v1

Overview
NameMS015288
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCalmodulin, putative
Locationscaffold2: 2318075 .. 2318524 (+)
RNA-Seq ExpressionMS015288
SyntenyMS015288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCATCCAATCCGACCAGCTGAAGCAGCTGCAAGACATTTTCAGGCGCTTCGACATGAACTCCGATGGCAGCCTCACGCAGCTCGAGCTCGGGGCGCTCCTCCGCTCCCTTGGAATCAAGCCCTCCGGCGACCAGCTCCACTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGAGGAACTGGTCAATGCCATTCTCCCCGATATGAACGACGATATCCTCGTCAATCAAGAACAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGACGGCAATGGCTACATCACCGCCTCCGAGCTCGCCGGTTCCATGGCCAAGATGGGCCAACCTCTTTCCTACCGCGAGCTCTCCGAGATGATGCGACTCGCCGACATCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTCATGGCCAAATCCGCCGCC

mRNA sequence

TCCATCCAATCCGACCAGCTGAAGCAGCTGCAAGACATTTTCAGGCGCTTCGACATGAACTCCGATGGCAGCCTCACGCAGCTCGAGCTCGGGGCGCTCCTCCGCTCCCTTGGAATCAAGCCCTCCGGCGACCAGCTCCACTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGAGGAACTGGTCAATGCCATTCTCCCCGATATGAACGACGATATCCTCGTCAATCAAGAACAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGACGGCAATGGCTACATCACCGCCTCCGAGCTCGCCGGTTCCATGGCCAAGATGGGCCAACCTCTTTCCTACCGCGAGCTCTCCGAGATGATGCGACTCGCCGACATCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTCATGGCCAAATCCGCCGCC

Coding sequence (CDS)

TCCATCCAATCCGACCAGCTGAAGCAGCTGCAAGACATTTTCAGGCGCTTCGACATGAACTCCGATGGCAGCCTCACGCAGCTCGAGCTCGGGGCGCTCCTCCGCTCCCTTGGAATCAAGCCCTCCGGCGACCAGCTCCACTCTCTCCTCTCCAACATGGATTCCAACGGCAACGGCTCCATCGAATTCGAGGAACTGGTCAATGCCATTCTCCCCGATATGAACGACGATATCCTCGTCAATCAAGAACAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGACGGCAATGGCTACATCACCGCCTCCGAGCTCGCCGGTTCCATGGCCAAGATGGGCCAACCTCTTTCCTACCGCGAGCTCTCCGAGATGATGCGACTCGCCGACATCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTCATGGCCAAATCCGCCGCC

Protein sequence

SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSEMMRLADIDGDGVISFNEFTTVMAKSAA
Homology
BLAST of MS015288 vs. NCBI nr
Match: XP_022149001.1 (probable calcium-binding protein CML16 [Momordica charantia])

HSP 1 Score: 288.9 bits (738), Expect = 2.5e-74
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE
Sbjct: 63  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMRLADIDGDGVISFNEFTTVMAKSAA
Sbjct: 123 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 152

BLAST of MS015288 vs. NCBI nr
Match: XP_022956672.1 (probable calcium-binding protein CML16 [Cucurbita moschata])

HSP 1 Score: 272.3 bits (695), Expect = 2.5e-69
Identity = 139/150 (92.67%), Postives = 146/150 (97.33%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           S+QSDQLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SLQSDQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEFEELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITASELAGSMAKMG PLSYRE
Sbjct: 63  IEFEELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMR AD DGDGVISFNEFTTVMA+SAA
Sbjct: 123 LSEMMRQADTDGDGVISFNEFTTVMARSAA 152

BLAST of MS015288 vs. NCBI nr
Match: XP_008446524.1 (PREDICTED: probable calcium-binding protein CML16 [Cucumis melo])

HSP 1 Score: 271.9 bits (694), Expect = 3.2e-69
Identity = 142/151 (94.04%), Postives = 146/151 (96.69%), Query Frame = 0

Query: 1   SIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 60
           SIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG
Sbjct: 3   SIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 62

Query: 61  SIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYR 120
           SIEF+ELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITA+ELAGSMAKMG PL+YR
Sbjct: 63  SIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 122

Query: 121 ELSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           ELSEMMR AD DGDGVISFNEFTTVMAKSAA
Sbjct: 123 ELSEMMRQADTDGDGVISFNEFTTVMAKSAA 153

BLAST of MS015288 vs. NCBI nr
Match: KAG6601337.1 (putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 271.2 bits (692), Expect = 5.5e-69
Identity = 138/150 (92.00%), Postives = 146/150 (97.33%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           S+QSDQLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SLQSDQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEF+ELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITASELAGSMAKMG PLSYRE
Sbjct: 63  IEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMR AD DGDGVISFNEFTTVMA+SAA
Sbjct: 123 LSEMMRQADTDGDGVISFNEFTTVMARSAA 152

BLAST of MS015288 vs. NCBI nr
Match: XP_004135127.1 (probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetical protein Csa_008611 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 7.2e-69
Identity = 141/151 (93.38%), Postives = 146/151 (96.69%), Query Frame = 0

Query: 1   SIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 60
           SIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG
Sbjct: 3   SIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 62

Query: 61  SIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYR 120
           SIEF+ELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITA+ELAGSMAKMG PL+YR
Sbjct: 63  SIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 122

Query: 121 ELSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           ELS+MMR AD DGDGVISFNEFTTVMAKSAA
Sbjct: 123 ELSDMMRQADTDGDGVISFNEFTTVMAKSAA 153

BLAST of MS015288 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 2.2e-57
Identity = 111/147 (75.51%), Postives = 132/147 (89.80%), Query Frame = 0

Query: 4   SDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 63
           +DQ+KQL+DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NGNGS+EF
Sbjct: 7   TDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEF 66

Query: 64  EELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSE 123
           +ELV AILPD+N+++L+NQEQL+EVFRSFDRDGNG ITA+ELAGSMAKMG PL+YREL+E
Sbjct: 67  DELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 126

Query: 124 MMRLADIDGDGVISFNEFTTVMAKSAA 151
           MM  AD +GDGVISFNEF+ +MAKSAA
Sbjct: 127 MMTEADSNGDGVISFNEFSHIMAKSAA 153

BLAST of MS015288 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.2e-56
Identity = 103/145 (71.03%), Postives = 133/145 (91.72%), Query Frame = 0

Query: 5   DQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFE 64
           DQ++QL+DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF+
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 65  ELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSEM 124
           ELV  ILPD+N+++L+N EQLLE+F+SFDRDGNG+I+A+ELAG+MAKMGQPL+Y+EL+EM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 125 MRLADIDGDGVISFNEFTTVMAKSA 150
           ++ AD +GDGVISF EF ++MAKSA
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSA 147

BLAST of MS015288 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.6e-50
Identity = 100/149 (67.11%), Postives = 126/149 (84.56%), Query Frame = 0

Query: 2   IQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSI 61
           ++  QLKQL+++FRRFDMN DGSLTQLEL ALLRSLG++P+GD++H+LL+ MD+NGNGS+
Sbjct: 18  LRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSV 77

Query: 62  EFEELVNAILPDMNDDI-LVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 121
           EF+EL  AI P +     LV+Q QLLEVFR+FDRDGNG+I+A+ELA SMA++GQPL++ E
Sbjct: 78  EFDELAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEE 137

Query: 122 LSEMMRLADIDGDGVISFNEFTTVMAKSA 150
           L+ MMR AD DGDGVISF EF  VMAKSA
Sbjct: 138 LTRMMRDADTDGDGVISFKEFAAVMAKSA 166

BLAST of MS015288 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 7.0e-43
Identity = 86/153 (56.21%), Postives = 123/153 (80.39%), Query Frame = 0

Query: 2   IQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKP-SGDQLHSLLSNMDSNGNGS 61
           ++ +QL+QL +IF RFD++ DGSLT+LEL ALLRSLG++P +GD++H+L++ +D++GNG+
Sbjct: 88  LRGEQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGT 147

Query: 62  IEFEELVNA----ILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPL 121
           +EF+EL ++    IL      + V+Q +L E FR+FDRDGNG+I+A+ELA SMA+MG P+
Sbjct: 148 VEFDELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPI 207

Query: 122 SYRELSEMMRLADIDGDGVISFNEFTTVMAKSA 150
            Y EL++MMR AD DGDG+ISF EFT +MAKSA
Sbjct: 208 CYAELTDMMREADTDGDGLISFEEFTAIMAKSA 240

BLAST of MS015288 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 7.5e-37
Identity = 80/146 (54.79%), Postives = 107/146 (73.29%), Query Frame = 0

Query: 5   DQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFE 64
           DQL +L++IFR FD N DGSLTQLELG+LLRSLG+KPS D+L SL+   D+N NG IEF 
Sbjct: 60  DQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEFS 119

Query: 65  ELVNAILPD-MNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSE 124
           E V  + P+ + D    +++Q+  +F  FDRDGNG+ITA+ELA SMAK+G  L+ +EL+ 
Sbjct: 120 EFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKELTG 179

Query: 125 MMRLADIDGDGVISFNEFTTVMAKSA 150
           M++ AD DGDG ISF EF+  +  +A
Sbjct: 180 MIKEADTDGDGRISFQEFSRAITAAA 205

BLAST of MS015288 vs. ExPASy TrEMBL
Match: A0A6J1D725 (probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC111017528 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.2e-74
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE
Sbjct: 63  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMRLADIDGDGVISFNEFTTVMAKSAA
Sbjct: 123 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 152

BLAST of MS015288 vs. ExPASy TrEMBL
Match: A0A6J1GX12 (probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC111458327 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.2e-69
Identity = 139/150 (92.67%), Postives = 146/150 (97.33%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           S+QSDQLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SLQSDQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEFEELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITASELAGSMAKMG PLSYRE
Sbjct: 63  IEFEELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMR AD DGDGVISFNEFTTVMA+SAA
Sbjct: 123 LSEMMRQADTDGDGVISFNEFTTVMARSAA 152

BLAST of MS015288 vs. ExPASy TrEMBL
Match: A0A1S3BF88 (probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.6e-69
Identity = 142/151 (94.04%), Postives = 146/151 (96.69%), Query Frame = 0

Query: 1   SIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 60
           SIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG
Sbjct: 3   SIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 62

Query: 61  SIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYR 120
           SIEF+ELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITA+ELAGSMAKMG PL+YR
Sbjct: 63  SIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 122

Query: 121 ELSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           ELSEMMR AD DGDGVISFNEFTTVMAKSAA
Sbjct: 123 ELSEMMRQADTDGDGVISFNEFTTVMAKSAA 153

BLAST of MS015288 vs. ExPASy TrEMBL
Match: A0A6J1JA36 (probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC111484890 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.5e-69
Identity = 138/150 (92.00%), Postives = 146/150 (97.33%), Query Frame = 0

Query: 1   SIQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 60
           S+QSDQLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS
Sbjct: 3   SMQSDQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGS 62

Query: 61  IEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRE 120
           IEF+ELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITASELAGSMAKMG PLSYRE
Sbjct: 63  IEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLSYRE 122

Query: 121 LSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           LSEMMR AD DGDGVISFNEFTTVMA+SAA
Sbjct: 123 LSEMMRQADTDGDGVISFNEFTTVMARSAA 152

BLAST of MS015288 vs. ExPASy TrEMBL
Match: A0A0A0KUE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.5e-69
Identity = 141/151 (93.38%), Postives = 146/151 (96.69%), Query Frame = 0

Query: 1   SIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 60
           SIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG
Sbjct: 3   SIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNG 62

Query: 61  SIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYR 120
           SIEF+ELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITA+ELAGSMAKMG PL+YR
Sbjct: 63  SIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 122

Query: 121 ELSEMMRLADIDGDGVISFNEFTTVMAKSAA 151
           ELS+MMR AD DGDGVISFNEFTTVMAKSAA
Sbjct: 123 ELSDMMRQADTDGDGVISFNEFTTVMAKSAA 153

BLAST of MS015288 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 223.0 bits (567), Expect = 1.6e-58
Identity = 111/147 (75.51%), Postives = 132/147 (89.80%), Query Frame = 0

Query: 4   SDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 63
           +DQ+KQL+DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NGNGS+EF
Sbjct: 7   TDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEF 66

Query: 64  EELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSE 123
           +ELV AILPD+N+++L+NQEQL+EVFRSFDRDGNG ITA+ELAGSMAKMG PL+YREL+E
Sbjct: 67  DELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 126

Query: 124 MMRLADIDGDGVISFNEFTTVMAKSAA 151
           MM  AD +GDGVISFNEF+ +MAKSAA
Sbjct: 127 MMTEADSNGDGVISFNEFSHIMAKSAA 153

BLAST of MS015288 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 219.2 bits (557), Expect = 2.3e-57
Identity = 103/145 (71.03%), Postives = 133/145 (91.72%), Query Frame = 0

Query: 5   DQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFE 64
           DQ++QL+DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF+
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 65  ELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSEM 124
           ELV  ILPD+N+++L+N EQLLE+F+SFDRDGNG+I+A+ELAG+MAKMGQPL+Y+EL+EM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 125 MRLADIDGDGVISFNEFTTVMAKSA 150
           ++ AD +GDGVISF EF ++MAKSA
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSA 147

BLAST of MS015288 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 151.4 bits (381), Expect = 5.9e-37
Identity = 79/145 (54.48%), Postives = 101/145 (69.66%), Query Frame = 0

Query: 5   DQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFE 64
           +QL +L++IFR FD N DGSLT+LELG+LLRSLG+KPS DQL +L+   D N NG +EF 
Sbjct: 16  EQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFS 75

Query: 65  ELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYRELSEM 124
           E V  + PD+        +QL  +FR FDRDGNGYITA+ELA SMAK+G  L+  EL+ M
Sbjct: 76  EFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTGM 135

Query: 125 MRLADIDGDGVISFNEFTTVMAKSA 150
           ++ AD DGDG I F EF   +  +A
Sbjct: 136 IKEADRDGDGCIDFQEFVQAITSAA 159

BLAST of MS015288 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 145.2 bits (365), Expect = 4.2e-35
Identity = 75/152 (49.34%), Postives = 103/152 (67.76%), Query Frame = 0

Query: 2   IQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSI 61
           +  +Q+ +L++IFR FD N DGSLTQLELG+LLR+LG+KPS DQ  +L+   D+  NG +
Sbjct: 9   LDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLV 68

Query: 62  EFEELVNAILPDM----NDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLS 121
           EF E V  + P++           +EQLL +FR FD DGNG+ITA+ELA SMAK+G  L+
Sbjct: 69  EFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALT 128

Query: 122 YRELSEMMRLADIDGDGVISFNEFTTVMAKSA 150
             EL+ M++ AD DGDG I+F EF   +  +A
Sbjct: 129 VAELTGMIKEADSDGDGRINFQEFAKAINSAA 160

BLAST of MS015288 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 122.1 bits (305), Expect = 3.8e-28
Identity = 60/144 (41.67%), Postives = 95/144 (65.97%), Query Frame = 0

Query: 2   IQSDQLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSI 61
           +  DQ+ + ++ F  FD + DG +T  ELG ++RSLG  P+  +L  +++ +D++GNG+I
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 64

Query: 62  EFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLSYREL 121
           +F E +N +   M D    ++E+L E FR FD+D NG+I+A+EL   M  +G+ LS  E+
Sbjct: 65  DFPEFLNLMARKMKD--TDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEV 124

Query: 122 SEMMRLADIDGDGVISFNEFTTVM 146
            EM+R AD+DGDG I++ EF  VM
Sbjct: 125 DEMIREADVDGDGQINYEEFVKVM 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149001.12.5e-74100.00probable calcium-binding protein CML16 [Momordica charantia][more]
XP_022956672.12.5e-6992.67probable calcium-binding protein CML16 [Cucurbita moschata][more]
XP_008446524.13.2e-6994.04PREDICTED: probable calcium-binding protein CML16 [Cucumis melo][more]
KAG6601337.15.5e-6992.00putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. s... [more]
XP_004135127.17.2e-6993.38probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetica... [more]
Match NameE-valueIdentityDescription
Q9LI842.2e-5775.51Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ... [more]
Q9FZ753.2e-5671.03Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ... [more]
Q6L5F41.6e-5067.11Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5ZD817.0e-4356.21Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9AWK27.5e-3754.79Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D7251.2e-74100.00probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GX121.2e-6992.67probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A1S3BF881.6e-6994.04probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 P... [more]
A0A6J1JA363.5e-6992.00probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC1114848... [more]
A0A0A0KUE03.5e-6993.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G25600.11.6e-5875.51Calcium-binding EF-hand family protein [more]
AT1G18530.12.3e-5771.03EF hand calcium-binding protein family [more]
AT3G03000.15.9e-3754.48EF hand calcium-binding protein family [more]
AT1G32250.14.2e-3549.34Calcium-binding EF-hand family protein [more]
AT5G21274.13.8e-2841.67calmodulin 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 84..112
e-value: 4.1E-4
score: 29.7
coord: 45..73
e-value: 1.4
score: 16.5
coord: 120..148
e-value: 7.0E-7
score: 38.9
coord: 9..37
e-value: 0.015
score: 24.5
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 8..69
e-value: 5.3E-12
score: 46.0
coord: 83..145
e-value: 7.9E-14
score: 51.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 5..40
score: 13.509171
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 116..150
score: 11.277538
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 41..76
score: 10.468573
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 80..115
score: 14.569197
IPR002048EF-hand domainCDDcd00051EFhcoord: 84..146
e-value: 6.35123E-18
score: 71.0397
NoneNo IPR availableGENE3D1.10.238.10coord: 1..150
e-value: 6.6E-44
score: 151.5
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 2..150
NoneNo IPR availablePANTHERPTHR23050:SF444CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 2..150
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 18..30
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 129..141
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 54..66
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 93..105
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 3..146

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS015288.1MS015288.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding